EF-hand domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008169 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13
Homology
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P32070|SM21_SCHMA (Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=SM21.7 PE=2 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 4.418e-23 Identity = 53/178 (29.78%), Postives = 89/178 (50.00%), Query Frame = 2 Query: 494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFI---KEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTA 1018 M+ F+ + + D E + ++L + R L V +WI +FD + D IT +E+C LGL NE RI R S ++LP+ V II S++ ++E+ + + +++ + P ++K + K ELE+ YG W V +W+ FS P S Q++ I L W+T Sbjct: 5 MEKFIQTYLTLLRDGDETVETSKLSESCRKEKLDMKQVNEWIALFDVDKDQKITFEEFCRGLGLKQNEMRIERNHIKTVQSGREQSLPEGVSIIASTMPKPKQVEVTQLFKDIYNEVKKDPDMNKVVKTFKSELERRYGRVWQVNAVTHSYWASFSHEPFQSIQFQYDNKIILAWRTP 182
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1) HSP 1 Score: 94.3597 bits (233), Expect = 1.691e-21 Identity = 58/181 (32.04%), Postives = 90/181 (49.72%), Query Frame = 2 Query: 494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSV--TSEMEI-----EMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 M+ F+ AF IDAD +E I EL KY + L + WI FD++ D I+++E+C GL +E R +++ E LP +++II +++ T + EI E VD N + +V+ +K L+ YG W V + G +W FS P S Q+K Y+ L W+T Sbjct: 10 MEEFIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGLKVSEIRREKDELKKERDGKFPKLPPNIEIIAATMSKTKQYEICCQFKEYVD---NTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSHEPFLSIQFKYNNYVCLAWRT 187
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267F2V4 (Dynein light chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g2 PE=3 SV=1) HSP 1 Score: 139.813 bits (351), Expect = 2.326e-36 Identity = 67/175 (38.29%), Postives = 109/175 (62.29%), Query Frame = 2 Query: 491 IMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 + D F+ A+F ID + EIT A+L +YMR NN +AFV+KW+ +FDS++ G ITLDEYC LGL EA+ ++ + + + +LP D+ +I S +T E+++ +++I +G ++K+IK++ + WH V + GQFW ++S P SF ++ GKYI+ IW+T Sbjct: 5 MTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGL---EAKKVKADYIAKMRS--GSLPSDITVISSDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRT 174
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H6V5 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g1 PE=3 SV=1) HSP 1 Score: 139.813 bits (351), Expect = 2.398e-36 Identity = 67/175 (38.29%), Postives = 109/175 (62.29%), Query Frame = 2 Query: 491 IMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 + D F+ A+F ID + EIT A+L +YMR NN +AFV+KW+ +FDS++ G ITLDEYC LGL EA+ ++ + + + +LP D+ +I S +T E+++ +++I +G ++K+IK++ + WH V + GQFW ++S P SF ++ GKYI+ IW+T Sbjct: 1 MTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGL---EAKKVKADYIAKMRS--GSLPSDITVISSDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRT 170
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8HXX8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028751g1 PE=4 SV=1) HSP 1 Score: 140.969 bits (354), Expect = 3.161e-36 Identity = 72/185 (38.92%), Postives = 109/185 (58.92%), Query Frame = 2 Query: 464 NRLLVFNCKIMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDA-FVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 R+ + FL AFF +D D+SE IT EL YM ++ +A F +KW+ +FD NDDG ITLDEYC+VLGL +ARI R LP+DV +I SS+ EM++++ +I + + ++ V+ +K++L+ +G WH V V GQ+W+ +S P SF +K G++I + WKT Sbjct: 31 GRVAAEEAAAQNPFLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRSNRLPEDVTVIASSMGLEMQVDLTEIFRDAQRRGKDEKDVAGRLKQKLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKT 215
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H730 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008204g1 PE=4 SV=1) HSP 1 Score: 140.969 bits (354), Expect = 3.333e-36 Identity = 72/185 (38.92%), Postives = 109/185 (58.92%), Query Frame = 2 Query: 464 NRLLVFNCKIMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDA-FVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 R+ + FL AFF +D D+SE IT EL YM ++ +A F +KW+ +FD NDDG ITLDEYC+VLGL +ARI R LP+DV +I SS+ EM++++ +I + + ++ V+ +K++L+ +G WH V V GQ+W+ +S P SF +K G++I + WKT Sbjct: 31 GRVAAEEAAAQNPFLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRNNRLPEDVTVIASSMGLEMQVDLTEIFRDAQRRGKDEKDVAGRLKQQLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKT 215
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8JCG4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008204g2 PE=4 SV=1) HSP 1 Score: 140.584 bits (353), Expect = 3.823e-36 Identity = 72/185 (38.92%), Postives = 109/185 (58.92%), Query Frame = 2 Query: 464 NRLLVFNCKIMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDA-FVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 R+ + FL AFF +D D+SE IT EL YM ++ +A F +KW+ +FD NDDG ITLDEYC+VLGL +ARI R LP+DV +I SS+ EM++++ +I + + ++ V+ +K++L+ +G WH V V GQ+W+ +S P SF +K G++I + WKT Sbjct: 31 GRVAAEEAAAQNPFLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRNNRLPEDVTVIASSMGLEMQVDLTEIFRDAQRRGKDEKDVAGRLKQQLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKT 215
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026622.1 (SMESG000026622.1) HSP 1 Score: 361.303 bits (926), Expect = 1.576e-126 Identity = 173/176 (98.30%), Postives = 175/176 (99.43%), Query Frame = 2 Query: 494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTAI 1021 MDNFLTAFFNID+DQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEEL+KAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT I Sbjct: 1 MDNFLTAFFNIDSDQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELDKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTEI 176
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000045029.1 (SMESG000045029.1) HSP 1 Score: 122.865 bits (307), Expect = 1.833e-33 Identity = 64/176 (36.36%), Postives = 95/176 (53.98%), Query Frame = 2 Query: 494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTAI 1021 MD+F+ AFF ID D S EI+ EL YM N+ + F+KKW+ IFD ++ G ITL+EYC VLG+ A + R ++P++VKI+QS++ + I +N S + + K + E L YG W C + GQ W+YFS S +I++I++TAI Sbjct: 1 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRM-------SMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSLIIHIDGFIFIIFRTAI 169
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000006950.1 (SMESG000006950.1) HSP 1 Score: 100.523 bits (249), Expect = 4.720e-25 Identity = 57/174 (32.76%), Postives = 95/174 (54.60%), Query Frame = 2 Query: 497 DNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNA-PQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 D F+ A+ ID D S IT EL YM NN ++ FV KW+ +FD ++ G ITL+E+C LGL ++ + ++ ++ SA +L + V I+ S++ E ++ + N+ + S +IK+ L+ +WHC+ GQ+W+Y+S P S K +YL+W+T Sbjct: 3 DAFIKAYCQIDKDGSGTITADELRAYMTENNYQEDFVTKWLRLFDRDNTGRITLEEFCETLGLEVSD--FIDKRTALKESA-DFSLYNGVNILSSNMVQPNEGIVIQVIRKARELYPNSFKEQSTYIKQNLDLVLETTWHCIITKGQYWAYYSCEPECSLAVKIENIVYLLWRT 173
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000042102.1 (SMESG000042102.1) HSP 1 Score: 98.9821 bits (245), Expect = 1.914e-24 Identity = 62/183 (33.88%), Postives = 97/183 (53.01%), Query Frame = 2 Query: 503 FLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTN---EARIMREQFNIEHSAIPKTLPD--DVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQ-------VSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 F+ AF ID+D S EIT EL +YMRL N K AFV+KW+NIFD+++ G ITL+E+C+ LGL+ N + RI + + E L D+ +I ++ + + ++I CQ A ++K++K L+ +G SW+ V + + S+ S P K K+ L+WK Sbjct: 3 FIKAFEEIDSDNSGEITRGELIRYMRLKNYKPAFVEKWLNIFDADNSGTITLEEFCNTLGLIPNKNYQKRIEKRKSEKELLKRKSLLIPLYDLYVIDEDPLNDPKFKDFCMKI-----CQEATNNENDFISIAKYLKSVLDVKFGRSWNVVVDIDAYGSFLSHEPGAFIHIKVRKFAILLWKV 180
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1) HSP 1 Score: 90.1225 bits (222), Expect = 3.043e-21 Identity = 60/177 (33.90%), Postives = 97/177 (54.80%), Query Frame = 2 Query: 497 DNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMRE--QFNIEHSAIPKT--LPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015 D F+ AFF+ID +++IT +L ++ NN + FV KW+N+FD GVITL+E+C VLGL E RE Q E + PK+ + DD II+ T E+ E D QN S+ +K+ +E+ Y +W + + GQ+W+++S P +F +K ++I+++ Sbjct: 6 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDD--IIEK--TKELRKEHPD---------QNKIVASE-LKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 168 The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008169 ID=SMED30008169|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1122bpback to top protein sequence of SMED30008169-orf-1 >SMED30008169-orf-1 ID=SMED30008169-orf-1|Name=SMED30008169-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=177bp MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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