EF-hand domain-containing protein

Overview


Name	EF-hand domain-containing protein
Smed ID	SMED30008169
Length (bp)	1122

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of EF-hand domain-containing protein (SMED30008169) t-SNE clustered cells

Violin plots show distribution of expression levels for EF-hand domain-containing protein (SMED30008169) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of EF-hand domain-containing protein (SMED30008169) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for EF-hand domain-containing protein (SMED30008169) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30008169

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
protonephridia	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30008169	h1SMcG0019151	Contig49405	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30008169	h1SMcG0019151	Contig49405	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
epidermis	SMED30008169	h1SMcG0019151	dd_Smed_v6_1881_0_1	dd_Smed_v6	PMID:30399335 Ross et al., 2018
Category 3 cell	SMED30008169	h1SMcG0019151	dd_Smed_v6_1881_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
epidermis	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
epidermis	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
whole organism	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
Category 4 cell	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
dorsal region of the epidermis	SMED30008169	h1SMcG0019151	dd_Smed_v4_1881_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P32070|SM21_SCHMA (Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=SM21.7 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 4.418e-23
Identity = 53/178 (29.78%), Postives = 89/178 (50.00%), Query Frame = 2

Query:  494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFI---KEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTA 1018
            M+ F+  +  +  D  E +  ++L +  R   L    V +WI +FD + D  IT +E+C  LGL  NE RI R       S   ++LP+ V II S++    ++E+  +  + +++ +  P ++K +   K ELE+ YG  W    V   +W+ FS  P  S Q++    I L W+T 
Sbjct:    5 MEKFIQTYLTLLRDGDETVETSKLSESCRKEKLDMKQVNEWIALFDVDKDQKITFEEFCRGLGLKQNEMRIERNHIKTVQSGREQSLPEGVSIIASTMPKPKQVEVTQLFKDIYNEVKKDPDMNKVVKTFKSELERRYGRVWQVNAVTHSYWASFSHEPFQSIQFQYDNKIILAWRTP 182

[Close]

BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.691e-21
Identity = 58/181 (32.04%), Postives = 90/181 (49.72%), Query Frame = 2

Query:  494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSV--TSEMEI-----EMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
            M+ F+ AF  IDAD +E I   EL KY +   L    +  WI  FD++ D  I+++E+C   GL  +E R  +++   E       LP +++II +++  T + EI     E VD   N      +  +V+  +K  L+  YG  W  V + G +W  FS  P  S Q+K   Y+ L W+T
Sbjct:   10 MEEFIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGLKVSEIRREKDELKKERDGKFPKLPPNIEIIAATMSKTKQYEICCQFKEYVD---NTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSHEPFLSIQFKYNNYVCLAWRT 187

[Close]

BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267F2V4 (Dynein light chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g2 PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.326e-36
Identity = 67/175 (38.29%), Postives = 109/175 (62.29%), Query Frame = 2

Query:  491 IMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
            + D F+ A+F ID +   EIT A+L +YMR NN  +AFV+KW+ +FDS++ G ITLDEYC  LGL   EA+ ++  +  +  +   +LP D+ +I S +T E+++ +++I  +G         ++K+IK++ +      WH V + GQFW ++S  P  SF ++ GKYI+ IW+T
Sbjct:    5 MTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGL---EAKKVKADYIAKMRS--GSLPSDITVISSDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRT 174

[Close]

BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H6V5 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g1 PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.398e-36
Identity = 67/175 (38.29%), Postives = 109/175 (62.29%), Query Frame = 2

Query:  491 IMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
            + D F+ A+F ID +   EIT A+L +YMR NN  +AFV+KW+ +FDS++ G ITLDEYC  LGL   EA+ ++  +  +  +   +LP D+ +I S +T E+++ +++I  +G         ++K+IK++ +      WH V + GQFW ++S  P  SF ++ GKYI+ IW+T
Sbjct:    1 MTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGL---EAKKVKADYIAKMRS--GSLPSDITVISSDMTLELQLRIIEIVRDGQRLNSMEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRT 170

[Close]

BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8HXX8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028751g1 PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 3.161e-36
Identity = 72/185 (38.92%), Postives = 109/185 (58.92%), Query Frame = 2

Query:  464 NRLLVFNCKIMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDA-FVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
             R+        + FL AFF +D D+SE IT  EL  YM  ++  +A F +KW+ +FD NDDG ITLDEYC+VLGL   +ARI R             LP+DV +I SS+  EM++++ +I  +   + ++   V+  +K++L+  +G  WH V V GQ+W+ +S  P  SF +K G++I + WKT
Sbjct:   31 GRVAAEEAAAQNPFLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRSNRLPEDVTVIASSMGLEMQVDLTEIFRDAQRRGKDEKDVAGRLKQKLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKT 215

[Close]

BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H730 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008204g1 PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 3.333e-36
Identity = 72/185 (38.92%), Postives = 109/185 (58.92%), Query Frame = 2

Query:  464 NRLLVFNCKIMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDA-FVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
             R+        + FL AFF +D D+SE IT  EL  YM  ++  +A F +KW+ +FD NDDG ITLDEYC+VLGL   +ARI R             LP+DV +I SS+  EM++++ +I  +   + ++   V+  +K++L+  +G  WH V V GQ+W+ +S  P  SF +K G++I + WKT
Sbjct:   31 GRVAAEEAAAQNPFLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRNNRLPEDVTVIASSMGLEMQVDLTEIFRDAQRRGKDEKDVAGRLKQQLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKT 215

[Close]

BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8JCG4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008204g2 PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 3.823e-36
Identity = 72/185 (38.92%), Postives = 109/185 (58.92%), Query Frame = 2

Query:  464 NRLLVFNCKIMDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDA-FVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
             R+        + FL AFF +D D+SE IT  EL  YM  ++  +A F +KW+ +FD NDDG ITLDEYC+VLGL   +ARI R             LP+DV +I SS+  EM++++ +I  +   + ++   V+  +K++L+  +G  WH V V GQ+W+ +S  P  SF +K G++I + WKT
Sbjct:   31 GRVAAEEAAAQNPFLDAFFAVDKDRSESITPDELAAYMTSSSQFEANFGEKWMKLFDENDDGAITLDEYCNVLGLNPKQARIYRLNQLKAECLRNNRLPEDVTVIASSMGLEMQVDLTEIFRDAQRRGKDEKDVAGRLKQQLDGQFGKLWHVVMVTGQYWASYSHEPGLSFFFKIGRHIVMCWKT 215

[Close]

BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026622.1 (SMESG000026622.1)

HSP 1 Score: 361.303 bits (926), Expect = 1.576e-126
Identity = 173/176 (98.30%), Postives = 175/176 (99.43%), Query Frame = 2

Query:  494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTAI 1021
            MDNFLTAFFNID+DQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEEL+KAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT I
Sbjct:    1 MDNFLTAFFNIDSDQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELDKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTEI 176

[Close]

BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000045029.1 (SMESG000045029.1)

HSP 1 Score: 122.865 bits (307), Expect = 1.833e-33
Identity = 64/176 (36.36%), Postives = 95/176 (53.98%), Query Frame = 2

Query:  494 MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKTAI 1021
            MD+F+ AFF ID D S EI+  EL  YM  N+ +  F+KKW+ IFD ++ G ITL+EYC VLG+    A + R            ++P++VKI+QS++        + I +N  S   +   + K + E L   YG  W C  + GQ W+YFS     S       +I++I++TAI
Sbjct:    1 MDSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGIEYKRASLQRRM-------SMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSLIIHIDGFIFIIFRTAI 169

[Close]

BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000006950.1 (SMESG000006950.1)

HSP 1 Score: 100.523 bits (249), Expect = 4.720e-25
Identity = 57/174 (32.76%), Postives = 95/174 (54.60%), Query Frame = 2

Query:  497 DNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNA-PQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
            D F+ A+  ID D S  IT  EL  YM  NN ++ FV KW+ +FD ++ G ITL+E+C  LGL  ++   + ++  ++ SA   +L + V I+ S++    E  ++ +         N+  + S +IK+ L+     +WHC+   GQ+W+Y+S  P  S   K    +YL+W+T
Sbjct:    3 DAFIKAYCQIDKDGSGTITADELRAYMTENNYQEDFVTKWLRLFDRDNTGRITLEEFCETLGLEVSD--FIDKRTALKESA-DFSLYNGVNILSSNMVQPNEGIVIQVIRKARELYPNSFKEQSTYIKQNLDLVLETTWHCIITKGQYWAYYSCEPECSLAVKIENIVYLLWRT 173

[Close]

BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000042102.1 (SMESG000042102.1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.914e-24
Identity = 62/183 (33.88%), Postives = 97/183 (53.01%), Query Frame = 2

Query:  503 FLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTN---EARIMREQFNIEHSAIPKTLPD--DVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQ-------VSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
            F+ AF  ID+D S EIT  EL +YMRL N K AFV+KW+NIFD+++ G ITL+E+C+ LGL+ N   + RI + +   E       L    D+ +I     ++ + +   ++I     CQ A         ++K++K  L+  +G SW+ V  +  + S+ S  P      K  K+  L+WK 
Sbjct:    3 FIKAFEEIDSDNSGEITRGELIRYMRLKNYKPAFVEKWLNIFDADNSGTITLEEFCNTLGLIPNKNYQKRIEKRKSEKELLKRKSLLIPLYDLYVIDEDPLNDPKFKDFCMKI-----CQEATNNENDFISIAKYLKSVLDVKFGRSWNVVVDIDAYGSFLSHEPGAFIHIKVRKFAILLWKV 180

[Close]

BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1)

HSP 1 Score: 90.1225 bits (222), Expect = 3.043e-21
Identity = 60/177 (33.90%), Postives = 97/177 (54.80%), Query Frame = 2

Query:  497 DNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSVLGLVTNEARIMRE--QFNIEHSAIPKT--LPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 1015
            D F+ AFF+ID   +++IT  +L ++   NN  + FV KW+N+FD    GVITL+E+C VLGL   E    RE  Q   E +  PK+  + DD  II+   T E+  E  D         QN    S+ +K+ +E+ Y  +W  + + GQ+W+++S  P  +F +K     ++I+++
Sbjct:    6 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDD--IIEK--TKELRKEHPD---------QNKIVASE-LKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 168

The following BLAST results are available for this feature:

BLAST of EF-hand domain-containing protein vs. Ensembl Human
BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
BLAST of EF-hand domain-containing protein vs. TrEMBL
BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
BLAST of EF-hand domain-containing protein vs. Planmine SMEST

BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|P32070\|SM21_SCHMA	4.418e-23	29.78	Antigen Sm21.7 OS=Schistosoma mansoni OX=6183 GN=S...	[more]
sp\|P14202\|TEGU_SCHMA	1.691e-21	32.04	Tegument antigen OS=Schistosoma mansoni OX=6183 GN...	[more]

BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A267F2V4	2.326e-36	38.29	Dynein light chain (Fragment) OS=Macrostomum ligna...	[more]
A0A1I8H6V5	2.398e-36	38.29	Dynein light chain OS=Macrostomum lignano OX=28230...	[more]
A0A1I8HXX8	3.161e-36	38.92	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8H730	3.333e-36	38.92	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8JCG4	3.823e-36	38.92	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]

BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000026622.1	1.576e-126	98.30	SMESG000026622.1	[more]
SMESG000045029.1	1.833e-33	36.36	SMESG000045029.1	[more]
SMESG000006950.1	4.720e-25	32.76	SMESG000006950.1	[more]
SMESG000042102.1	1.914e-24	33.88	SMESG000042102.1	[more]
SMESG000026288.1	3.043e-21	33.90	SMESG000026288.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30008169 ID=SMED30008169|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1122bp
ATGTCTTTTCATTTTAAGCCAAATATTTGAATCGCTGCTTAAAATCTAAT
ATCAGACTTTGTGAAGGTCTGGATCTGTTTCACAGAAGCTGCTGTCAAAA
TCGTTTGAGAAAAGCATTGAATTAAATACAAGTTTCACCTCGTTCCTGGA
TATTGTTTGAAATGTTGATAGATTTGTCGACTTATTCTAAAAATCTCGCA
CCTATTCTATTGGAATTCTGAGATTGTGAGTCTGTGTGCGCGGAGGTGGG
ACTGTGTGTAGGCGGGTATTTGTGCGGTCATTGAAATTGTGTGCGTCGCA
TTGGCGATGAATTAATGTAGCATTTTCTTGTTCATCATTCCGTAAAGAAC
ATTGATCTGCACATCAAAAGCTATTTTCGGCATATAGTTGAGAATGGCTA
ATAAAACAATGCACAGACAGATTTCTATATAGTATATACATGCATTTTTT
ATAATAAAAACAGAATCGCTTGCTAGTGTTTAATTGTAAAATCATGGATA
ATTTTCTAACAGCTTTTTTCAACATTGACGCAGATCAGAGTGAAGAGATT
ACTATTGCGGAGTTGGAGAAGTATATGCGTTTGAACAACTTGAAAGACGC
TTTTGTGAAGAAATGGATCAACATTTTTGATTCAAACGATGATGGAGTGA
TTACTTTGGATGAATACTGTTCAGTTTTGGGCCTTGTAACAAATGAAGCG
CGAATTATGAGAGAACAATTTAACATTGAACATTCTGCAATTCCAAAGAC
TTTACCTGATGATGTGAAAATTATTCAAAGCTCAGTGACGAGCGAAATGG
AAATAGAAATGGTTGACATAGAAATTAATGGTTGGAGCAAATGTCAGAAT
GCTCCACAAGTTTCTAAATTCATCAAAGAAGAGTTAGAAAAAGCTTATGG
GCCCTCTTGGCATTGTGTAACAGTTCTCGGTCAATTTTGGAGCTATTTTT
CTTCAGCTCCAGCTACTTCGTTCCAATATAAACGAGGAAAATATATTTAT
CTTATTTGGAAAACTGCAATTTAAATAGTTATTTTTTTAATGAAATATGT
TGACCTTTAATTTAAATTAGATTGTTTTATTTCAATTTTCTAGTGTCTTA
AGAATTTATTAATATTGATTTT

protein sequence of SMED30008169-orf-1

>SMED30008169-orf-1 ID=SMED30008169-orf-1|Name=SMED30008169-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=177bp
MDNFLTAFFNIDADQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDD
GVITLDEYCSVLGLVTNEARIMREQFNIEHSAIPKTLPDDVKIIQSSVTS
EMEIEMVDIEINGWSKCQNAPQVSKFIKEELEKAYGPSWHCVTVLGQFWS
YFSSAPATSFQYKRGKYIYLIWKTAI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000018	Category 4 cell
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000035	Category 3 cell
PLANA:0000054	dorsal region of the epidermis
PLANA:0000136	whole organism
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR037177	DLC_sf
IPR002048	EF_hand_dom
IPR001372	Dynein_light_chain_typ-1/2
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **biological process**
Term	Definition
GO:0007017	microtubule-based process

Vocabulary: **cellular component**
Term	Definition
GO:0030286	dynein complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001372	Dynein light chain, type 1/2	SMART	SM01375	Dynein_light_2	coord: 86..174 e-value: 2.7E-9 score: 46.9
IPR001372	Dynein light chain, type 1/2	PFAM	PF01221	Dynein_light	coord: 91..174 e-value: 4.5E-16 score: 58.9
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 1..69 e-value: 3.6E-11 score: 44.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 6..60 e-value: 1.1E-9 score: 38.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 33..68 score: 11.78
IPR037177	Dynein light chain superfamily	GENE3D	G3DSA:3.30.740.10		coord: 80..175 e-value: 4.6E-13 score: 50.9
IPR037177	Dynein light chain superfamily	SUPERFAMILY	SSF54648	DLC	coord: 86..174
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 46..58
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 4..66

Main menu

EF-hand domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

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