Usp domain-containing protein

Overview
NameUsp domain-containing protein
Smed IDSMED30008163
Length (bp)858
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Usp domain-containing protein (SMED30008163) t-SNE clustered cells

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30008163) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Usp domain-containing protein (SMED30008163) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30008163) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008163

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30008163SMESG000039918.1 dd_Smed_v4_2433_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30008163SMESG000039918.1 dd_Smed_v4_2433_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30008163SMESG000039918.1 dd_Smed_v4_2433_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008163SMESG000039918.1 SMESG000039917.1 Contig40830newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008163SMESG000039918.1 SMESG000039917.1 Contig40830uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008163SMESG000039918.1 SMESG000039917.1 Contig50973newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008163SMESG000039918.1 SMESG000039917.1 Contig50973uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A068Y8K9 (Universal stress protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000873800 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 3.198e-39
Identity = 59/144 (40.97%), Postives = 106/144 (73.61%), Query Frame = 2 
Query:  182 SSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISIS-DTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHA 610
            ++ RN+L P+D+S++  RAF+WY+AN+++ GDV+F VH++EP Y T AI ++ ++    ++DMT  M+ +    K+L QKY+++A++  +  K  ++VD KAG AI+K A +++  +++IG+RG+G ++RT LGS SD+I+++A
Sbjct:    2 AAGRNVLFPIDASENCERAFKWYEANLRKPGDVVFFVHVIEPVYTTPAIGLAMESPPLMVDDMTRVMEESIAAGKKLGQKYMQMAKESNLDCKAFLHVDTKAGAAIVKSAIDHKADIIVIGSRGLGAIKRTFLGSVSDYIVHNA 145
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: U6IX26 (Universal stress protein OS=Echinococcus granulosus OX=6210 GN=EGR_06697 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 3.198e-39
Identity = 59/144 (40.97%), Postives = 106/144 (73.61%), Query Frame = 2 
Query:  182 SSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISIS-DTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHA 610
            ++ RN+L P+D+S++  RAF+WY+AN+++ GDV+F VH++EP Y T AI ++ ++    ++DMT  M+ +    K+L QKY+++A++  +  K  ++VD KAG AI+K A +++  +++IG+RG+G ++RT LGS SD+I+++A
Sbjct:    2 AAGRNVLFPIDASENCERAFKWYEANLRKPGDVVFFVHVIEPVYTTPAIGLAMESPPLMVDDMTRVMEESIAAGKKLGQKYMQMAKESNLDCKAFLHVDTKAGAAIVKSAIDHKADIIVIGSRGLGAIKRTFLGSVSDYIVHNA 145
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A0X3Q2U4 (Universal stress protein MJ0531 OS=Schistocephalus solidus OX=70667 GN=Y531 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 2.480e-38
Identity = 61/149 (40.94%), Postives = 104/149 (69.80%), Query Frame = 2 
Query:  170 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISIS-DTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            MA   S RN+LIP+D+S +  RAFEWY   +K++ D++F VH++EP Y T A+ ++ ++    ++DMT  M+ +    K+L  KY+++A+D  +  K  ++VD K G+AI+K AS+++   ++IG+RG+G ++RT LGS SD+I+++AN
Sbjct:    1 MASGDSQRNVLIPIDASPNCLRAFEWYNTQLKKKNDLVFFVHVIEPVYTTPAVGLAMESPPLLVDDMTRVMEESISSGKKLGHKYMQMAKDVGLDCKAFLHVDTKPGNAIVKSASDHKADFIVIGSRGLGAIKRTFLGSVSDYIVHNAN 149
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A0R3U2V2 (Usp domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS832 PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 7.581e-38
Identity = 56/145 (38.62%), Postives = 107/145 (73.79%), Query Frame = 2 
Query:  182 SSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISIS-DTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            +++R +L P+D+SD+ +RAF+WY+ N+++  D+++ VH++EP Y T AI ++ ++    ++DMT  M+ +    K+L QKY+++A+D+ I  K  ++VD K G AI+K A++++  +++IG+RG+G ++RT LGS SD+I+++A+
Sbjct:    2 ATSRRVLFPIDASDNCQRAFKWYEVNLRKPNDLVYFVHVIEPVYTTPAIGLAMESPPLMVDDMTRVMEESIAAGKKLGQKYMQMAKDENIECKAFLHVDTKPGAAIVKSATDHKADIIVIGSRGLGAIKRTFLGSVSDYIVHNAS 146
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A183SK28 (Usp domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS4576 PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 7.064e-37
Identity = 61/149 (40.94%), Postives = 104/149 (69.80%), Query Frame = 2 
Query:  170 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISIS-DTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            MA   S RN+LIP+D+S +  RAFEWY   +K++ D++F VH++EP Y T A+ ++ ++    ++DMT  M+ +    K+L  KY+++A+D  +  K  ++VD K G+AI+K AS+++   ++IG+RG+G ++RT LGS SD+I+++AN
Sbjct:    1 MASGDSQRNVLIPIDASPNCLRAFEWYNTQLKKKNDLVFFVHVIEPVYTTPAVGLAMESPPLLVDDMTRVMEESISSGKKLGHKYMQMAKDVGLDCKAFLHVDTKPGNAIVKSASDHKADFIVIGSRGLGAIKRTFLGSVSDYIVHNAN 149
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO33143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST34])

HSP 1 Score: 102.449 bits (254), Expect = 5.465e-27
Identity = 46/145 (31.72%), Postives = 85/145 (58.62%), Query Frame = 2 
Query:  188 NRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKM---EDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
             R ILIPVD S+HS RAF+WY   +   GD + +VH +E       + +     F     E+ ++ +    ++ + +L+ Y  + ++ ++H + ++ V   AGD I ++A +   +L+++G RG G +RRTILGS SD++++H++
Sbjct:    3 GRKILIPVDGSEHSERAFDWYAELLHSPGDEVLVVHCIE----LPPVPLEHQFPFVFAYYEEWSAMVKETREQHEAMLRSYEDICKEKKLHYEIMMVVGKPAGDVICQVARDVSANLIVLGTRGQGMIRRTILGSVSDYVVHHSH 143
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO45800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRQ2])

HSP 1 Score: 99.7525 bits (247), Expect = 6.297e-26
Identity = 53/142 (37.32%), Postives = 85/142 (59.86%), Query Frame = 2 
Query:  191 RNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINV-DNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            R IL+P+DSS HS  AFEWY  N+  E D L LVH+++     + +S    +    ++  ++M+   K+ K L +KY   AE   +  K  I V   K G+ I + + +    L+++G+RG+G++RRTILGS SD++L+HA+
Sbjct:   10 RRILLPIDSSKHSEDAFEWYVNNMHHEEDELILVHVLDSAAIQTRVSSHGLVD---DEFKNEMNKGLKEVKALEEKYKTKAETASLKAK--IEVRGGKPGETICQCSKDEHCDLILMGSRGLGSIRRTILGSVSDYVLHHAH 146
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO38238 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDJ5])

HSP 1 Score: 96.6709 bits (239), Expect = 8.239e-25
Identity = 48/144 (33.33%), Postives = 82/144 (56.94%), Query Frame = 2 
Query:  182 SSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            S   +IL PVD SDHS RAF++Y   +K+  D + L HIVEP       +++  +     +  + M    + A+E+   Y K+ E + I  + I    N AG+ I ++A       ++IGNRG+G+++RT+LGS +D+++ H++
Sbjct:    2 SKTESILFPVDGSDHSSRAFDYYLDKVKRADDQVLLAHIVEPT-GIPTPTLAHGVTRSRAEWDTIMRRMEETAREITADYEKICEAENIPFQSIWGAGN-AGEGICELAKNEGADFILIGNRGLGSIKRTLLGSVTDYVVQHSH 143
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUS8])

HSP 1 Score: 93.5893 bits (231), Expect = 1.325e-23
Identity = 46/143 (32.17%), Postives = 85/143 (59.44%), Query Frame = 2 
Query:  191 RNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVE-PQYATSAISISDTIKFKMEDMTSK-MDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            R ++IPVD S HS RAF WY+ ++ + GD + ++H  E P   +S  +   T+   + D  +K +D     AK+L+++Y K  ++  +  + +     + G+ I ++A +     V++G+RG GT+RRT+LGS SD+ ++H++
Sbjct:    7 RRVVIPVDGSQHSERAFNWYRQHVHEPGDEVLIIHTQEQPTIPSSPYAYGGTV---LPDEWNKAVDECIVNAKKLIEEYNKKCKEQGMTCR-LFKGSGQPGETICQLAKDLSAKHVVMGSRGCGTIRRTLLGSVSDYCVHHSS 145
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO38142 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDN9])

HSP 1 Score: 92.0485 bits (227), Expect = 7.945e-23
Identity = 51/154 (33.12%), Postives = 83/154 (53.90%), Query Frame = 2 
Query:  182 SSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDD----------RIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            S  R +++ +D S +S RA++WY  NI QEGD+L ++H  E     +A        +  E+ +S +  A  +AK LL+   +  ++            IH K +     K G+ + K A +    L+I+G+RG+GTLRRT LGS SD+ ++HA+
Sbjct:    7 SCKRKVVLALDGSVNSMRAYQWYWDNIYQEGDLLLVIHAFELPTMPAAPYPYGFAYY--EEWSSLVQKADDEAKHLLEDCGRKCQEKICSIDPEKKKNIHFK-LFKETGKPGEVVCKFAQDENAHLIIMGSRGLGTLRRTFLGSNSDYCVHHAH 157
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000039918.1 (SMESG000039918.1)

HSP 1 Score: 301.982 bits (772), Expect = 1.151e-104
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 2 
Query:  170 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN
Sbjct:    1 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 148
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000039918.1 (SMESG000039918.1)

HSP 1 Score: 239.965 bits (611), Expect = 8.332e-81
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 2 
Query:  170 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENR 520
            MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENR
Sbjct:    1 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENR 117
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000016283.1 (SMESG000016283.1)

HSP 1 Score: 149.058 bits (375), Expect = 1.119e-44
Identity = 65/143 (45.45%), Postives = 101/143 (70.63%), Query Frame = 2 
Query:  185 SNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            + R+I+IP+DSS+HS+RAF WY  N+KQ  D++  +H+VEP YAT A+ + +     ++DM+  M+ A  + K+L Q+++  A+D  +  K  I+VD K G A++K A +++  ++IIGNRG+GTLRRT LGS SD IL+HA+
Sbjct:    2 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAH 144
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000022085.1 (SMESG000022085.1)

HSP 1 Score: 128.257 bits (321), Expect = 1.441e-36
Identity = 56/148 (37.84%), Postives = 94/148 (63.51%), Query Frame = 2 
Query:  170 MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            M  +   R +L+P+D SD+S RAFEWY   + + GD+  L++IVEP YAT  ++ ++      + ++ +M    +  K+L Q+++ +A++  +  K  I+VD K G A++K A E+R  ++++GNRG+  L RT LGS SD+IL HA+
Sbjct:    1 MYSKKDTRVVLLPLDGSDNSERAFEWYIGQMHRRGDLAVLINIVEPVYATPPLNSAENSVLSNDSVSQQMQDIIEDGKQLTQRFLHMAKNANVEAKAFIHVDPKPGPALVKCAKEHRADVIVVGNRGVSKLHRTFLGSVSDYILQHAH 148
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020952.1 (SMESG000020952.1)

HSP 1 Score: 127.487 bits (319), Expect = 3.607e-36
Identity = 59/142 (41.55%), Postives = 94/142 (66.20%), Query Frame = 2 
Query:  191 RNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKF-KMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHAN 613
            R IL+PVD S+H+ RA +WY  N+ +  D++ ++HIVEP Y ++A+ I   + F  +ED+ SK+     K K L++K+    ++     KG + VD+K G+AIIK+   ++V L+I+ NRG G +RRT LGS SD++L+HA+
Sbjct:   11 RVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHAD 152
The following BLAST results are available for this feature:
BLAST of Usp domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A068Y8K93.198e-3940.97Universal stress protein OS=Echinococcus multilocu... [more]
U6IX263.198e-3940.97Universal stress protein OS=Echinococcus granulosu... [more]
A0A0X3Q2U42.480e-3840.94Universal stress protein MJ0531 OS=Schistocephalus... [more]
A0A0R3U2V27.581e-3838.62Usp domain-containing protein OS=Mesocestoides cor... [more]
A0A183SK287.064e-3740.94Usp domain-containing protein OS=Schistocephalus s... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO331435.465e-2731.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO458006.297e-2637.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO382388.239e-2533.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447461.325e-2332.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO381427.945e-2333.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000039918.11.151e-104100.00SMESG000039918.1[more]
SMESG000039918.18.332e-81100.00SMESG000039918.1[more]
SMESG000016283.11.119e-4445.45SMESG000016283.1[more]
SMESG000022085.11.441e-3637.84SMESG000022085.1[more]
SMESG000020952.13.607e-3641.55SMESG000020952.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008163 ID=SMED30008163|Name=Usp domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=858bp
ACATTAACCTTCAAAATCTTTACTATAATTTATTTATTTTTTCAAATAAC
AAAATTGCCATTATCATTTTTTTAAACTATAAGCTATAATATAATAAAAG
GTTAACACTGTGAAAGTATTGATTTATCAGAAACCGTGATTGAAGTTTAT
TTTCTGAATTTCCATTAAAATGGCCCATGAAAGTAGTAATAGGAATATTT
TAATTCCAGTCGATTCTAGTGACCATTCTAGGCGTGCATTTGAATGGTAT
CAGGCAAATATCAAACAAGAAGGAGATGTTTTGTTTTTGGTTCACATAGT
TGAACCTCAATATGCTACTTCAGCCATTTCTATTTCTGATACCATAAAAT
TTAAAATGGAAGATATGACTTCAAAAATGGATTCTGCATCGAAAAAAGCG
AAAGAGTTACTGCAAAAGTATATTAAACTTGCGGAAGATGACAGAATTCA
TACGAAGGGCATCATTAATGTCGATAATAAAGCTGGAGATGCTATCATTA
AAATTGCATCAGAAAATAGAGTTAGTCTCGTTATTATTGGTAATCGAGGT
ATTGGAACTCTTCGTAGAACTATACTTGGAAGTACTAGTGATTTTATCTT
AAATCATGCCAATGTTCCTGTTATCATTGTGCCACCACCTAAATGATCGT
GATTATTCAAACGTAATTGTAAGGTGCATGTATGAAATTGATTTTTCGAT
TATTATTTATAATATATCATCTATAAACATATTGATTGAATTTATAGATT
CTTGTTAGAAAAATCATTGCCAGCTGATATTTAATTGATTGCACCTTTAT
TAATTAAAATATATCTTGAGTTTGTTAATTTTTAAAGTTCATTATAAAAT
ATTTCAAC
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protein sequence of SMED30008163-orf-1

>SMED30008163-orf-1 ID=SMED30008163-orf-1|Name=SMED30008163-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
MAHESSNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYAT
SAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIIN
VDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHANVP
VIIVPPPK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR006015Universal_stress_UspA
IPR014729Rossmann-like_a/b/a_fold
IPR006016UspA
Vocabulary: biological process
TermDefinition
GO:0006950response to stress
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 63..90
NoneNo IPR availablePANTHERPTHR46989FAMILY NOT NAMEDcoord: 8..157
NoneNo IPR availableCDDcd00293USP_Likecoord: 9..154
e-value: 3.91788E-29
score: 101.678
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 8..157
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 115..127
score: 35.08
coord: 7..25
score: 31.16
coord: 133..155
score: 35.48
IPR006016UspAPFAMPF00582Uspcoord: 7..155
e-value: 1.2E-25
score: 90.7
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 2..158
e-value: 1.1E-33
score: 118.9