Cysteine-rich PDZ-binding protein

Overview
NameCysteine-rich PDZ-binding protein
Smed IDSMED30008097
Length (bp)381
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cysteine-rich PDZ-binding protein (SMED30008097) t-SNE clustered cells

Violin plots show distribution of expression levels for Cysteine-rich PDZ-binding protein (SMED30008097) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cysteine-rich PDZ-binding protein (SMED30008097) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cysteine-rich PDZ-binding protein (SMED30008097) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008097

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30008097 SmedASXL_026281SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30008097 SmedASXL_026281SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30008097 Contig918newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008097 Contig918uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
muscle cellSMED30008097 dd_Smed_v4_3121_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Human
Match: CRIPT (CXXC repeat containing interactor of PDZ3 domain [Source:HGNC Symbol;Acc:HGNC:14312])

HSP 1 Score: 96.2857 bits (238), Expect = 1.452e-26
Identity = 54/103 (52.43%), Postives = 71/103 (68.93%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL  V+TP+      R  TE  S   KLNENK LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGTVITPDTWKDGARNTTE--SGGRKLNENKALTSK-KARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Celegans
Match: C36B1.14 (pep chromosome:WBcel235:I:8730156:8730922:1 gene:WBGene00044614.1 transcript:C36B1.14.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C36B1.14)

HSP 1 Score: 70.8626 bits (172), Expect = 5.004e-17
Identity = 42/104 (40.38%), Postives = 64/104 (61.54%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPNAKRTE--------TERKSVVSKLNENKLLTLKSRSRNTPYGRTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT 325
            MVC  C+ KL K+V  +  R +        +  K+V +K   N+L+ ++ ++  T  G  CKLCK  +H  G++YC+ CA++KGIC+MCGKK+ +TK L  STT
Sbjct:    1 MVCGDCEKKLTKIVGVDPYRNKKVNRNADGSGPKTVTTK---NRLIGVQKKA--TIVGAKCKLCKMLIHQPGSHYCSTCAYQKGICAMCGKKIQNTKGLRQSTT 99
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Fly
Match: CG4537 (gene:FBgn0032153 transcript:FBtr0113031)

HSP 1 Score: 87.0409 bits (214), Expect = 2.869e-23
Identity = 46/99 (46.46%), Postives = 64/99 (64.65%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPNAKRTETERKSVVSKLNENKLLTLKSRSRNTPYG---RTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT 325
            MVC+KC+ KL KV  PN  RT T   +   K+NENK L+  +R R  P G     C++C+  VH  G++YC  CA+KK IC+MCGKK+++TK+   S+T
Sbjct:    1 MVCEKCEAKLSKVSAPNPWRTSTA-PAGGRKINENKALS-SARERYNPIGTALPPCRICRQKVHQMGSHYCQACAYKKAICAMCGKKIMNTKNYKQSST 97
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Zebrafish
Match: cript (cysteine-rich PDZ-binding protein [Source:NCBI gene;Acc:550592])

HSP 1 Score: 99.7525 bits (247), Expect = 4.123e-28
Identity = 54/103 (52.43%), Postives = 73/103 (70.87%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK+LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYGKSGFATCRICKSSVHQSGSHYCQGCAYKKGICAMCGKKVIDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Xenopus
Match: cript (CXXC repeat containing interactor of PDZ3 domain [Source:NCBI gene;Acc:549548])

HSP 1 Score: 94.3597 bits (233), Expect = 8.971e-26
Identity = 53/103 (51.46%), Postives = 70/103 (67.96%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRT----CKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVCDKC+ KL  V+TP+      R  TE  S   KLNENK LT K+ +R  PYG+     C++CK SVH  G++YC GCA+KKGIC+MCGKKV++TK+   ++
Sbjct:    1 MVCDKCEKKLGTVITPDTWKSGARNTTE--SGGRKLNENKALTSKT-ARFDPYGKNKFAICRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLETKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Xenopus
Match: cript (CXXC repeat containing interactor of PDZ3 domain [Source:NCBI gene;Acc:549548])

HSP 1 Score: 85.5001 bits (210), Expect = 2.323e-22
Identity = 49/101 (48.51%), Postives = 67/101 (66.34%), Query Frame = 2 
Query:   44 CDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRT----CKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            C+ C+ KL  V+TP+      R  TE  S   KLNENK LT K+ +R  PYG+     C++CK SVH  G++YC GCA+KKGIC+MCGKKV++TK+   ++
Sbjct:    4 CNYCEKKLGTVITPDTWKSGARNTTE--SGGRKLNENKALTSKT-ARFDPYGKNKFAICRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLETKNYKQTS 101
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Xenopus
Match: cript (CXXC repeat containing interactor of PDZ3 domain [Source:NCBI gene;Acc:549548])

HSP 1 Score: 82.0333 bits (201), Expect = 6.550e-21
Identity = 49/103 (47.57%), Postives = 68/103 (66.02%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRT----CKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            +VC + + KL  V+TP+      R  TE  S   KLNENK LT K+ +R  PYG+     C++CK SVH  G++YC GCA+KKGIC+MCGKKV++TK+   ++
Sbjct:    1 LVCRR-EKKLGTVITPDTWKSGARNTTE--SGGRKLNENKALTSKT-ARFDPYGKNKFAICRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLETKNYKQTS 99
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Xenopus
Match: cript (CXXC repeat containing interactor of PDZ3 domain [Source:NCBI gene;Acc:549548])

HSP 1 Score: 81.6481 bits (200), Expect = 7.468e-21
Identity = 47/95 (49.47%), Postives = 63/95 (66.32%), Query Frame = 2 
Query:   62 KLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRT----CKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            KL  V+TP+      R  TE  S   KLNENK LT K+ +R  PYG+     C++CK SVH  G++YC GCA+KKGIC+MCGKKV++TK+   ++
Sbjct:    6 KLGTVITPDTWKSGARNTTE--SGGRKLNENKALTSKT-ARFDPYGKNKFAICRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLETKNYKQTS 97
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Mouse
Match: Cript (cysteine-rich PDZ-binding protein [Source:MGI Symbol;Acc:MGI:1929655])

HSP 1 Score: 97.8265 bits (242), Expect = 3.069e-27
Identity = 54/103 (52.43%), Postives = 72/103 (69.90%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKALTSK-KARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. UniProt/SwissProt
Match: sp|Q567Z6|CRIPT_DANRE (Cysteine-rich PDZ-binding protein OS=Danio rerio OX=7955 GN=cript PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 3.112e-27
Identity = 54/103 (52.43%), Postives = 73/103 (70.87%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK+LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYGKSGFATCRICKSSVHQSGSHYCQGCAYKKGICAMCGKKVIDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. UniProt/SwissProt
Match: sp|Q792Q4|CRIPT_RAT (Cysteine-rich PDZ-binding protein OS=Rattus norvegicus OX=10116 GN=Cript PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.147e-26
Identity = 54/103 (52.43%), Postives = 72/103 (69.90%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKALTSK-KARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. UniProt/SwissProt
Match: sp|O70333|CRIPT_MOUSE (Cysteine-rich PDZ-binding protein OS=Mus musculus OX=10090 GN=Cript PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.147e-26
Identity = 54/103 (52.43%), Postives = 72/103 (69.90%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKALTSK-KARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. UniProt/SwissProt
Match: sp|Q9P021|CRIPT_HUMAN (Cysteine-rich PDZ-binding protein OS=Homo sapiens OX=9606 GN=CRIPT PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 6.973e-26
Identity = 54/103 (52.43%), Postives = 71/103 (68.93%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL  V+TP+      R  TE  S   KLNENK LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGTVITPDTWKDGARNTTE--SGGRKLNENKALTSK-KARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. UniProt/SwissProt
Match: sp|Q3ZC66|CRIPT_BOVIN (Cysteine-rich PDZ-binding protein OS=Bos taurus OX=9913 GN=CRIPT PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 6.973e-26
Identity = 54/103 (52.43%), Postives = 71/103 (68.93%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL  V+TP+      R  TE  S   KLNENK LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGTVITPDTWKDGARNTTE--SGGRKLNENKALTSK-KARFDPYGKNKFSTCRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. TrEMBL
Match: A0A553QMM2 (Uncharacterized protein OS=Danionella translucida OX=623744 GN=DNTS_000553 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.530e-25
Identity = 55/103 (53.40%), Postives = 73/103 (70.87%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL KV+TP+      R  TE  S   KLNENK+LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGKVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYGKSAFATCRICKSSVHQSGSHYCQGCAYKKGICAMCGKKVIDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. TrEMBL
Match: A0A4W4GDN2 (Uncharacterized protein OS=Electrophorus electricus OX=8005 GN=cript PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 4.250e-25
Identity = 55/103 (53.40%), Postives = 72/103 (69.90%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVCDKC+ KL +V+TP+      R  TE  S   KLNENK+LT K + R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCDKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KGRFDPYGKSGFATCRICKSSVHQSGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. TrEMBL
Match: A0A498LS98 (Cysteine-rich PDZ-binding OS=Labeo rohita OX=84645 GN=ROHU_031271 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 9.224e-25
Identity = 54/103 (52.43%), Postives = 73/103 (70.87%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK+LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYGKSGFATCRICKSSVHQSGSHYCQGCAYKKGICAMCGKKVIDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. TrEMBL
Match: B2GPQ6 (Cript protein OS=Danio rerio OX=7955 GN=cript PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 9.224e-25
Identity = 54/103 (52.43%), Postives = 73/103 (70.87%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL +V+TP+      R  TE  S   KLNENK+LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGRVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYGKSGFATCRICKSSVHQSGSHYCQGCAYKKGICAMCGKKVIDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. TrEMBL
Match: C3XZA7 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_113645 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.696e-24
Identity = 55/102 (53.92%), Postives = 73/102 (71.57%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYG--RTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT 325
            MVC+KCQ KL KV+TP+      R  TE      ++NENKLLT K ++R +PY   R C++CK +VH +G++YC+GCA+KKGIC+MCGKKV+DT S   STT
Sbjct:    1 MVCEKCQKKLGKVITPDPWKDGARNTTEGGG--RRVNENKLLTQK-KNRFSPYQEFRKCRICKTTVHQKGSHYCHGCAYKKGICAMCGKKVLDTASYRQSTT 99
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Cavefish
Match: cript (cysteine-rich PDZ-binding protein [Source:NCBI gene;Acc:550592])

HSP 1 Score: 89.7373 bits (221), Expect = 3.158e-24
Identity = 50/95 (52.63%), Postives = 66/95 (69.47%), Query Frame = 2 
Query:   62 KLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGR----TCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            KL KV+TP+      R  TE  S   KLNENK+LT K ++R  PYG+    TC++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    8 KLGKVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYGKSGFATCRICKSSVHQAGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 99
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Nematostella
Match: EDO33267 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSQ8])

HSP 1 Score: 88.9669 bits (219), Expect = 2.042e-24
Identity = 51/102 (50.00%), Postives = 66/102 (64.71%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYG--RTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT 325
            MVCDKCQ KL KV+TP+      R  TE      K+ ENKLLT K ++R  PY   + C++CK +VH   + YC GCA+KKGIC+MCGKK++DT S   S+ 
Sbjct:    1 MVCDKCQKKLGKVITPDPWKSGARNNTEGGG--RKVGENKLLTQK-KNRFNPYAEFKKCRICKDTVHQAHSNYCQGCAYKKGICAMCGKKILDTSSYRQSSA 99
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Medaka
Match: cript (CXXC repeat containing interactor of PDZ3 domain [Source:NCBI gene;Acc:101161117])

HSP 1 Score: 96.6709 bits (239), Expect = 5.311e-27
Identity = 54/103 (52.43%), Postives = 72/103 (69.90%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPN----AKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRT----CKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMST 322
            MVC+KC+ KL KV+TP+      R  TE  S   KLNENK+LT K ++R  PY +T    C++CK SVH  G++YC GCA+KKGIC+MCGKKV+DTK+   ++
Sbjct:    1 MVCEKCEKKLGKVITPDTWKDGARNTTE--SGGRKLNENKMLTSK-KARFDPYSKTGFAICRICKSSVHQPGSHYCQGCAYKKGICAMCGKKVLDTKNYKQTS 100
[Close]
BLAST of Cysteine-rich PDZ-binding protein vs. Planmine SMEST
Match: SMESG000051979.1 (SMESG000051979.1)

HSP 1 Score: 192.2 bits (487), Expect = 8.241e-65
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 2 
Query:   38 MVCDKCQIKLKKVVTPNAKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT 325
            MVCDKCQIKLKKVVTPNAKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT
Sbjct:    1 MVCDKCQIKLKKVVTPNAKRTETERKSVVSKLNENKLLTLKSRSRNTPYGRTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDTKSLNMSTT 96
The following BLAST results are available for this feature:
BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CRIPT1.452e-2652.43CXXC repeat containing interactor of PDZ3 domain [... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C36B1.145.004e-1740.38pep chromosome:WBcel235:I:8730156:8730922:1 gene:W... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG45372.869e-2346.46gene:FBgn0032153 transcript:FBtr0113031[more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cript4.123e-2852.43cysteine-rich PDZ-binding protein [Source:NCBI gen... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
cript8.971e-2651.46CXXC repeat containing interactor of PDZ3 domain [... [more]
cript2.323e-2248.51CXXC repeat containing interactor of PDZ3 domain [... [more]
cript6.550e-2147.57CXXC repeat containing interactor of PDZ3 domain [... [more]
cript7.468e-2149.47CXXC repeat containing interactor of PDZ3 domain [... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Cript3.069e-2752.43cysteine-rich PDZ-binding protein [Source:MGI Symb... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q567Z6|CRIPT_DANRE3.112e-2752.43Cysteine-rich PDZ-binding protein OS=Danio rerio O... [more]
sp|Q792Q4|CRIPT_RAT2.147e-2652.43Cysteine-rich PDZ-binding protein OS=Rattus norveg... [more]
sp|O70333|CRIPT_MOUSE2.147e-2652.43Cysteine-rich PDZ-binding protein OS=Mus musculus ... [more]
sp|Q9P021|CRIPT_HUMAN6.973e-2652.43Cysteine-rich PDZ-binding protein OS=Homo sapiens ... [more]
sp|Q3ZC66|CRIPT_BOVIN6.973e-2652.43Cysteine-rich PDZ-binding protein OS=Bos taurus OX... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A553QMM23.530e-2553.40Uncharacterized protein OS=Danionella translucida ... [more]
A0A4W4GDN24.250e-2553.40Uncharacterized protein OS=Electrophorus electricu... [more]
A0A498LS989.224e-2552.43Cysteine-rich PDZ-binding OS=Labeo rohita OX=84645... [more]
B2GPQ69.224e-2552.43Cript protein OS=Danio rerio OX=7955 GN=cript PE=2... [more]
C3XZA71.696e-2453.92Uncharacterized protein OS=Branchiostoma floridae ... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cript3.158e-2452.63cysteine-rich PDZ-binding protein [Source:NCBI gen... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO332672.042e-2450.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cript5.311e-2752.43CXXC repeat containing interactor of PDZ3 domain [... [more]
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BLAST of Cysteine-rich PDZ-binding protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000051979.18.241e-65100.00SMESG000051979.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008097 ID=SMED30008097|Name=Cysteine-rich PDZ-binding protein|organism=Schmidtea mediterranea sexual|type=transcript|length=381bp
TTTTAACATATTTAAAATATATAATTGTCAATATAATATGGTATGCGATA
AATGCCAAATTAAATTAAAAAAAGTTGTTACTCCAAATGCTAAACGGACT
GAAACGGAACGGAAAAGTGTTGTATCAAAATTAAATGAAAACAAATTGCT
AACTTTAAAATCCAGAAGCAGAAATACTCCATATGGAAGAACATGTAAAT
TATGTAAATGTAGTGTTCATTTAGAAGGTGCATACTATTGCAATGGATGC
GCTTTCAAGAAGGGAATATGTTCTATGTGTGGTAAAAAAGTCGTAGACAC
AAAATCTTTGAATATGAGTACTACATAAGAATTGCATTTTATCTCACCTC
GACATCACTTGAATGATTCTATCAATATTGC
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protein sequence of SMED30008097-orf-1

>SMED30008097-orf-1 ID=SMED30008097-orf-1|Name=SMED30008097-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
FNIFKIYNCQYNMVCDKCQIKLKKVVTPNAKRTETERKSVVSKLNENKLL
TLKSRSRNTPYGRTCKLCKCSVHLEGAYYCNGCAFKKGICSMCGKKVVDT
KSLNMSTT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR019367PDZ-binding_CRIPT
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019367PDZ-binding protein, CRIPTPFAMPF10235Criptcoord: 24..106
e-value: 6.2E-21
score: 74.8
IPR019367PDZ-binding protein, CRIPTPANTHERPTHR11805FAMILY NOT NAMEDcoord: 13..105