Reverse transcriptase domain-containing protein

Overview
NameReverse transcriptase domain-containing protein
Smed IDSMED30008076
Length (bp)2550
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Reverse transcriptase domain-containing protein (SMED30008076) t-SNE clustered cells

Violin plots show distribution of expression levels for Reverse transcriptase domain-containing protein (SMED30008076) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Reverse transcriptase domain-containing protein (SMED30008076) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Reverse transcriptase domain-containing protein (SMED30008076) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008076

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30008076SMESG000024306.1 Contig631GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30008076SMESG000024306.1 Contig631GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30008076SMESG000060194.1 Contig3762GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30008076SMESG000008737.1 Contig631GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30008076SMESG000008737.1 Contig631GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30008076SMESG000008737.1 Contig3762GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
gutSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
posterior region of the whole animalSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
lateral region of the whole animalSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30008076SMESG000008737.1 dd_Smed_v4_540_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30008076SMESG000008737.1 dd_Smed_v6_540_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30008076SMESG000008737.1 dd_Smed_v6_540_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A267E6B6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010566g1 PE=4 SV=1)

HSP 1 Score: 471.855 bits (1213), Expect = 1.602e-151
Identity = 288/742 (38.81%), Postives = 409/742 (55.12%), Query Frame = 2
Query:  218 KVNIIFMNHLDVGYSDHLAGL--TGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSN--VVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLE----DFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIE--ISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQ-----PEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSNLT---GVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFT 2389
            +V++IFMNHLDVGY+    G+  TGY  NVLN YF+VH+P+A+ L  Q++  G+ E FRYTTHPWLL  +LNCP +L L  I++QCPN+  L  M  A+  G ++WH GPMNM  ENMN   LE SLNIS  L+++F   K +RV+S RDVPG++   I  L KNN+V ++ GVN  S PP VT     R       E +   I+ + +P GYP+D G +P NPGG+  +DC    G  E+LCF FR DN GPPQS+ EVLN+YE++R ++  A++ A T E+F       ++L+ VV  E+GDTWIQG ASDP+K A +RA+ R F      G    ++  V DA R++IKP EHT+GL S  D  N+RN  F   R     + +E +W+EQR +  L +      HPL+  + +   +L     D  G          + S+      +   Y F            F DG+I  L+++  +   +    L K +Y +Y+E D     K  ++Y N GYGKPN T NA+P S EW+++    YR+ D+  SF+ ++ PED  A S YG P  V + ++    ++ I  +V   NK  TR+ E + ++F+      +      +W L+K+   + P  V   GS  QH +++  VF      +K V  V   D  + CP  +        A +FP    LT   GV+FN YNNVW TNYI+WYP+       D  FKARF+
Sbjct:   32 RVHVIFMNHLDVGYN----GIPDTGYINNVLNRYFHVHFPQALSLVKQIREGGFRERFRYTTHPWLLDMYLNCP-DLTLNGIQLQCPNSTDLEAMKDALTLGDVVWHAGPMNMQIENMNGALLEASLNISRRLDEKFQPEKKIRVLSQRDVPGMTSAAIPVLKKNNIVAVSVGVNPASYPPEVT-----REPFVWLDELSQESIVGIWHPGGYPNDPGDSPANPGGISVKDCSMVSGVPEILCFSFRTDNQGPPQSVAEVLNSYEILRDQFNGAEVIASTLEDFVQAILPYQSLMQVVRTEIGDTWIQGIASDPRKMAEYRALSRAFTRCRSDGACSGNDPQVQDAVRYMIKPPEHTWGLPSQPDQNNYRNVDFNKARSGSAYVQNENAWLEQRMFNTLAVQA-AKGHPLSSYMAQEMSQLTPTLPDMTGY--------VKQSLQPDTIFEYGYYRFSF----------FSDGSIDHLQINGGQSFANQRGRLAKFVYRSYDELDDN---KLLRSYGNPGYGKPNITANANPLSLEWRMLATDFYRKPDN-SSFVIVVAPEDSLAVSDYGAPQKVFLTYNF---SSGIDVDVLVWNKTSTRIEEGLFVEFRASADMADAPNASCRWLLNKVDGYVDPMRVTYGGSILQHAINEHAVFSCEMGASKKVLVVQSKDVPLACPFSR-----FQEASVFPYLDRLTRVDGVSFNIYNNVWITNYIFWYPYHTG----DQNFKARFS 728          
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A267G5B8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012712g1 PE=4 SV=1)

HSP 1 Score: 469.159 bits (1206), Expect = 1.899e-150
Identity = 287/742 (38.68%), Postives = 410/742 (55.26%), Query Frame = 2
Query:  218 KVNIIFMNHLDVGYSDHLAGL--TGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSN--VVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLE----DFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIE--ISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQ-----PEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSNLT---GVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFT 2389
            +V++IFMNHLDVGY+    G+  TGY  NVLN YF+VH+P+A+ L  Q++  G+ E FRYTTHPWLL  +LNCP +L+L  I++QCPN+  L  M  A+  G I+WH GPMNM  ENM    LE SLNIS  L+++F   K +RV+S RDVPG++   I  L KNN+V ++ GVN  S PP VT     R       E +   I+ + +P GYP++ G +P NPGG+  +DC    G  E+LCF FR DN GPPQS+ EVLN+YE++R ++  A++ A T E+F       ++L+ VV  E+GDTWIQG ASDP+K A +RA+ R F      G    ++  V DA R++IKP EHT+GL S  D  N+RN  F   R     + +E +W+EQR +  L +      HPL+  + +   +L     D  G          + S+      +   Y F            F DG+I  L+++  +   +  + L K +Y +Y+E D     K  ++Y N GYGKPN T NA+P S EW+++    YR+ D+  SF+ ++ PED  A S YG P  V + ++    ++ I  +V   NK  TR+ E + ++F+      +      +W L+K+   + P  V   GS  QH +++  VF      +K V  V   D  + CP  +        A +FP    LT   GV+FN YNNVW TNYI+WYP+       D  FKARF+
Sbjct:   32 RVHVIFMNHLDVGYN----GIPDTGYINNVLNRYFHVHFPQALSLVKQIREGGFRERFRYTTHPWLLDMYLNCP-DLILNGIQLQCPNSTDLEAMKDALTLGDIVWHAGPMNMQIENMKGALLEASLNISRRLDEKFQPEKKIRVLSQRDVPGMTSAAIPVLKKNNIVAVSVGVNPASYPPEVT-----REPFVWLDELSQESIVGIWHPGGYPNEPGNSPANPGGISVKDCSMVSGVPEILCFSFRTDNQGPPQSVAEVLNSYEILRDQFNGAEVIASTLEDFVQAILPYQSLMQVVRTEIGDTWIQGIASDPRKMAEYRALSRAFTRCRSDGACSGNDPQVQDAVRYMIKPPEHTWGLPSQPDQNNYRNVDFNKARSGSAYVQNENAWLEQRMFNTLAVQA-AKGHPLSSYMAQEMSQLTPTLPDMTGY--------VKQSLQPDAIFEYGYYRFSF----------FSDGSIDHLQINGGQSFANQRDRLAKFVYRSYDELDDN---KLLRSYGNPGYGKPNITANANPLSLEWRMLATDFYRKPDN-SSFVIVVVPEDSLAVSDYGAPQKVFLTYNF---SSGIDVDVLVWNKTSTRIEEGLFVEFRASADMADAPNASCRWLLNKVDGYVDPMRVTYGGSILQHAINEHAVFSCEMGASKKVLVVQSKDVPLACPFSR-----FQEASVFPYLDRLTRVDGVSFNIYNNVWITNYIFWYPYHTG----DQNFKARFS 728          
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: K1QIM2 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10017740 PE=4 SV=1)

HSP 1 Score: 463.381 bits (1191), Expect = 2.982e-148
Identity = 286/740 (38.65%), Postives = 415/740 (56.08%), Query Frame = 2
Query:  209 DVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSN--VVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRL--EDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEF-KDGAITSL--KMDNIEISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDS---PCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSN-LTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQ 2395
            +V  V++IFMNHLDVGY D ++   G+  N+LN YFN ++P+A++L+ QL++  YVE F YTTHPWL+S +L+CP++  L++I + CPN         AI+ G I WH GPMNM  EN+N    ++SLN+S  L+K+F F +    +S RDVPGL++ VI +L++  +  ++ GVN G++PP V  L RW    +        E++   +P GYP   GPNP  PGG+  +DC   PG    LC+ F  DN GPP  + E+L+ YE++R  + NAK+   TFEE+F    K R+ LPV+  EVGDTWIQG ASDP K A +RA+          G    S+  +V ASR L+K  EHT+GL S  D++NW N QF   R     +  E +W EQR++  + +D L    PL   I +A  +L   + +  EY      ++              +F+C +    + + F KDG++ SL    + +E +   PLG+ +Y TYNE++     K Y  Y   GY K NST NAHPES+ W  V+  +Y+   S    C  L  L   +P++ S+YG P SV + +       +++ +  W NK PTRL E+I   F P       +W LSK+G  + P +VI NGSQ+ H VD+   +I++        +++  D  +     K   P+ +P PL PI+ N +TGVAFN YNN+W+TNYI WYP+       D  FKARF  +
Sbjct:   16 NVKYVHVIFMNHLDVGY-DGISPEIGFINNILNRYFNEYFPRAVNLSEQLRAGEYVENFVYTTHPWLVSLYLDCPEHFRLSDIPLLCPNQTEKEYFEAAIRRGDITWHAGPMNMQPENINEILFQMSLNMSFELDKKFNFQRSFPTLSQRDVPGLTQAVIPSLVQRGIAAVSVGVNPGTSPPAVPPLFRWNFEGK--------EVMATWHPGGYPLGPGPNPARPGGLSTKDCVVIPGFEHALCYAFIQDNGGPPIDVLEILSNYEILRKEFPNAKIKGSTFEEYFAEVNKIRDSLPVIRQEVGDTWIQGIASDPYKMANYRAVSEAITECVMAGLCKASDPRIVSASRLLVKLPEHTWGLPSVSDNVNWSNPQFNKARLGDHYVNCEKAWQEQREFIWMAIDQLRDMEPLYQMIQQALSKLLPREPNLREYEIVSDMSK--------------TFKCED---GMAIRFGKDGSLQSLYDPYNKMEWATGAPLGQFLYVTYNETNFVDMAKQYNYYGGAGYNKKNSTKNAHPESRPWFAVVSKMYQAKPSGPGTCDILLKLVMAEPKSHSWYGAPQSVWINYKSRAKGFDVTLQ--WMNKTPTRLPESIMYYFSPA-QKEGLEWRLSKVGHLVDPGNVILNGSQYVHGVDNGVYYINNFGQG---LQLLTRDVPLVSIATKQRPPSPFPVPLKPISQNDITGVAFNLYNNIWDTNYILWYPYHDGLNSSD--FKARFQIK 721          
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A267GEQ6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018835g2 PE=4 SV=1)

HSP 1 Score: 461.07 bits (1185), Expect = 6.290e-147
Identity = 284/762 (37.27%), Postives = 423/762 (55.51%), Query Frame = 2
Query:  200 PEPDVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFEN-MNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVT------KLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMG--NPITSNVVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYK--TYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPI-TSNLTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQVNLVT----EKVDDTLRY 2437
            P P++T+V+++FMNHLDVG+S  L    GY+ANV+NTY NV++ +A + +  L+  GY+ETF Y  HPW+L  F +CP+N+ L  IK+QCP+ A ++ + QA+K G I WH G MN+ +E  +    L++SL+ +  L+ +FG  + +  VSLRDVPGL+R  I  + KN +V +T+G+N   A P+V       K  RWR+           EI Y + P GYP + GP+PN+PGG+   +C +APG+TE+ C+ FR DN+GPP   +EVL+ YEV+RG+Y NA + A T E+F+ +   NR+ +PV   E GDTW+QG  +D  + AR+RA+ R  +     G     + +++  SR++ K AEHTYGL+   D +++ N  F ++ Q         SW+EQRQ+ QL +D   P   L   I      L D    E+ G G +         +L +  +    T       V  +   +    +D+     +   G  +Y TYNESDT FFL+YY      NTG+GK NST NAHP S+ W I   +IY+ S S  +F      +DP+++  YG P S+  ++  N +    +   +  +K PTRLMEA  I F P+  G      L+K+G  I+   V  NGS WQH V  S   ++     K   RV   D+ + CP    YGPTVWP P+ P+  S + G++FN  NNVW+ N+++WYP+M        RF+      ++ +T     +VD  L +
Sbjct:   30 PHPEITRVHLMFMNHLDVGFS--LPSEIGYSANVINTYLNVYFERAANFSRDLRKFGYLETFSYLAHPWMLEMFFDCPQNVSLQGIKIQCPSQATVDYVAQAVKEGAIQWHAGSMNVQYETCLTPNLLQISLDSAKRLDAKFGIKRKLSAVSLRDVPGLTRAAIDLMAKNGIVAVTNGINW--ACPSVDLGSPDLKFVRWRSPDTG------NEIFYNKIPGGYPGNPGPDPNHPGGLDFSNCLQAPGTTEIQCWAFRSDNSGPPVDYKEVLSNYEVLRGQYPNAAISASTMEKFWSLVLANRDSVPVFTKENGDTWLQGDQADFHRMARYRAIARALDTCVSAGACQLESPDMIMFSRWIAKAAEHTYGLNGVWDYVHYSNLDFYSVMQNDNFRHGVSSWLEQRQFLQLAVDAAPPK--LASYIAAELAPLAD----EFPGVGANFS-------RLNDTAWGRPLTAGGPYRVVFDRRAGLARFSIDSGATWHQGG-GAFVYRTYNESDTAFFLRYYTNPNGDNTGFGKYNSTQNAHPVSQFWSISAAAIYQHS-SQSTFAVQFLMDDPQSSVKYGAPASMWAMY--NFTGTGYTLHSWSLSKTPTRLMEAFYIDF-PKPPGALA--VLNKLGHDIAVGQVAANGSVWQHMVGHSPYSVAFRQQGKAELRVQSLDSSLACPRTASYGPTVWPVPMSPVPDSKVAGISFNMLNNVWDVNFVFWYPYMPTEANFRARFRVDLADHLDDLTVDGEHQVDAKLNW 761          
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A1I8H8Q3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 460.685 bits (1184), Expect = 1.558e-146
Identity = 281/753 (37.32%), Postives = 419/753 (55.64%), Query Frame = 2
Query:  200 PEPDVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFEN-MNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVT------KLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMG--NPITSNVVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYK--TYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPI-TSNLTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQVNLVTEKVD 2422
            P P++T+V+++FMNHLDVG+S  L    GY+ANV+N Y NV++ +A +++  L+  GY+ETF Y  HPW+L  F +CP+N+ L  IK+QCP+ A ++ + QA+K G I WH G MN+ +E  +    L++SL+ +  L+ +FG  + +  VSLRDVPGL+R  I  + KN +V +T+G+N   A P+V       K  RWR+           EI Y + P GYP + GP+PN+PGG+   +C +APG+TE+ C+ FR DN+GPP   +EVL+ YEV+RG+Y NA + A T E+F+ +   NR+ +PV   E GDTW+QG  +D  + AR+RA+ R  +     G     + +++  SR++ K AEHTYGL+   D +++ N  F ++ Q         SW+EQRQ+ QL +D   P   L   I      L D    E+ G G +         +L +  +    T       V  +   +    +D+     +   G  +Y TYNESDT FFL+YY      NTG+GK NST NAHP S+ W I   +IY+ S S  +F      +DP+++  YG P S+  ++  N +    +   +  +K PTRLMEA  I F P+  G      L+K+G  I+   V  NGS WQH V  S   ++     K   RV   D+ + CP    YGPTVWP P+ P+  S + G++FN  NNVW+ N+++WYP+M        RF+      ++ +T   D
Sbjct:   46 PHPEITRVHLMFMNHLDVGFS--LPSEIGYSANVINAYLNVYFERAANVSRDLRKFGYLETFSYLAHPWMLEMFFDCPQNVSLQGIKIQCPSQATVDYVAQAVKEGAIQWHAGSMNVQYETCLTPNLLQISLDSAKRLDAKFGIKRKLSAVSLRDVPGLTRAAIDLMAKNGIVAVTNGINW--ACPSVDLGSPDLKFVRWRSPDTG------NEIFYNKIPGGYPGNPGPDPNHPGGLDFSNCLQAPGTTEIQCWAFRSDNSGPPVDYKEVLSNYEVLRGQYPNAAISASTMEKFWSLVLANRDSVPVFTKENGDTWLQGDQADFHRMARYRAIARALDTCVSAGACQLESPDMIMFSRWIAKAAEHTYGLNGVWDYVHYSNLDFYSVMQNDNFRHGVSSWLEQRQFLQLAVDAAPPK--LASYIAAELAPLAD----EFPGVGANFS-------RLNDTAWGRPLTAGGPYRVVFDRRAGLARFSIDSGATWHQGG-GAFVYRTYNESDTAFFLRYYTNPNGDNTGFGKYNSTQNAHPVSQFWSISAAAIYQHS-SQSTFAVQFLMDDPQSSVKYGAPASMWAMY--NFTGTGYTLHSWSLSKTPTRLMEAFYIDF-PKPPGALA--VLNKLGHDIAVGQVAANGSVWQHMVGHSPYSVAFRQQGKAELRVQSLDSSLACPRTASYGPTVWPVPMSPVPHSKVAGISFNMLNNVWDVNFVFWYPYMPTEANFRARFRVDLADHLDDLTVDGD 768          
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000008737.1 (SMESG000008737.1)

HSP 1 Score: 1595.1 bits (4129), Expect = 0.000e+0
Identity = 780/782 (99.74%), Postives = 781/782 (99.87%), Query Frame = 2
Query:   98 VNSLFLLFVFYFSIIELSEGFGKSPPKNVKEDVIPEPDVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSNVVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSNLTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQVNLVTEKVDDTLRYYI 2443
            VNSLFLLFVFYFSIIELSEGFGKSPPKNVKE VIPEPDVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVGPNPNNPGGMK+QDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSNVVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSNLTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQVNLVTEKVDDTLRYYI
Sbjct:    4 VNSLFLLFVFYFSIIELSEGFGKSPPKNVKEAVIPEPDVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVGPNPNNPGGMKQQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSNVVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSNLTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQVNLVTEKVDDTLRYYI 785          
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000008741.1 (SMESG000008741.1)

HSP 1 Score: 1170.61 bits (3027), Expect = 0.000e+0
Identity = 556/780 (71.28%), Postives = 661/780 (84.74%), Query Frame = 2
Query:   98 VNSLFLLFVFYFSIIELSEGFGKSPPKNVKEDVIPEPDVTKVNIIFMNHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFRYTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDGPMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVIRTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQNPWGYPDDVXXXXXXXXXMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQEVLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQGTASDPKKFARFRAMQREFEFYYQMGNPITSNVVDASRFLIKPAEHTYGLDSCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLTISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHSITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATSYYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGNQFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIPWDTGITCPIIKGYGPTVWPAPLFPITSNLTGVAFNFYNNVWNTNYIYWYPFMGPGTQLDNRFKARFTTQVNLVTEKVDDTLRY 2437
             N L L F+F  ++  L++GFG  P    +E VIPEPDVTKVNIIFMNHLDVGYSD++A L G++ANVLN YFN+H+P A++LA +++  GYVETFRYTTHPWLLSFFL+CP NL LANIK+QCPN+ +L +M+QAI+N  I+WHDGPMNMNFEN+NAFGLE++LNISANLN++FG  KP+RV+SLRDVPGL+RG IRTL+KNNVVGITSGVNSGSAPPNV KLCRWRAA+RSF E+NIPE++YMQNP GYP++ GPNPNNP GM   +CFRAP STEVLCF FR DN+GPPQS++EVLNAYE+VR RYINA LYAGT EEFF+I W+NRNLLP+ DNE+GDTWIQGTASDPKKFARFR MQREFE YY+ G  I++NV++A+ +LIKPAEHTYG +  GD+INWRNDQ+ NI ++  V++S+LSW+EQRQYQQLVLD+LG  H LT+SILKAFK LE +  ++               F  Q+ PY + C+NNTHSI V F++GAI+ L+ +N++IS   PLGK+IY TYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIV++++YR+ + PCSFLCIL PED +A++YYGFPDSV + WDINI+   ISTEV  +NK PTRLME+IS+ FQP++V N  QWY SKMGEPISPYSVI+NGSQWQHNVDDSGVFISSAPD+ PVFRVIPWDTG++CPII+GYGPTVWPAPL PITSNLTGVAFNFYNNVWNTNYI+WYPF G  T LDN+FKARFTT VNLVT+KVDDTLRY
Sbjct:    2 TNVLLLSFIFS-TLTYLTQGFGVKPKSYTEEKVIPEPDVTKVNIIFMNHLDVGYSDYMAHLPGFSANVLNIYFNMHFPNAVNLARKMRIGGYVETFRYTTHPWLLSFFLHCPPNLTLANIKIQCPNSTSLASMLQAIENRDIIWHDGPMNMNFENLNAFGLEIALNISANLNRKFGINKPIRVLSLRDVPGLTRGAIRTLIKNNVVGITSGVNSGSAPPNVPKLCRWRAAKRSFNEKNIPEVIYMQNPHGYPNNPGPNPNNPDGMSADNCFRAPKSTEVLCFMFRTDNSGPPQSVEEVLNAYEIVRARYINANLYAGTLEEFFNIVWQNRNLLPLFDNEIGDTWIQGTASDPKKFARFRVMQREFERYYKSGAQISANVINAASYLIKPAEHTYGTEGVGDNINWRNDQWANIIKRNDVISSQLSWVEQRQYQQLVLDLLGYNHSLTLSILKAFKELETYTNLK--------------EFMAQSKPYIYTCSNNTHSIDVGFENGAISYLQAENLKISENFPLGKIIYQTYNESDTEFFLKYYKTYWNTGYGKPNSTVNAHPESKEWKIVLRTMYRKPNHPCSFLCILSPEDSKASTYYGFPDSVFIYWDINIANGHISTEVILNNKHPTRLMESISVVFQPKVVENNHQWYFSKMGEPISPYSVIQNGSQWQHNVDDSGVFISSAPDSPPVFRVIPWDTGLSCPIIEGYGPTVWPAPLSPITSNLTGVAFNFYNNVWNTNYIFWYPFKGSNTTLDNQFKARFTTHVNLVTKKVDDTLRY 766          
The following BLAST results are available for this feature:
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E6B61.602e-15138.81Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267G5B81.899e-15038.68Uncharacterized protein OS=Macrostomum lignano OX=... [more]
K1QIM22.982e-14838.65Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A267GEQ66.290e-14737.27Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8H8Q31.558e-14637.32Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008737.10.000e+099.74SMESG000008737.1[more]
SMESG000008741.10.000e+071.28SMESG000008741.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008076 ID=SMED30008076|Name=Reverse transcriptase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2550bp
ATTGTATGCAACTCTTGAGGTTGTAGTTTTAAATTATAAATAAATTTAAA
AACTCAATTTATTCATTTTAACATTGAATAATTAATTCATGATTATTGTG
AATAGCCTATTCTTATTGTTTGTTTTTTATTTCAGTATAATAGAATTATC
TGAAGGATTTGGAAAATCACCGCCAAAGAATGTGAAGGAAGATGTTATAC
CAGAGCCTGATGTTACTAAAGTCAACATCATATTCATGAATCATTTAGAT
GTCGGCTACAGTGATCATTTAGCGGGACTAACTGGATACACTGCAAACGT
TTTGAATACGTATTTTAATGTCCATTATCCAAAAGCGATTGATTTAGCTA
ATCAATTAAAGTCTCAGGGTTATGTTGAAACATTCCGATATACGACTCAT
CCTTGGTTGTTATCTTTTTTTCTGAATTGTCCCAAAAATCTGGTATTGGC
AAACATTAAAGTTCAGTGTCCAAATGCAGCCGCATTAAATACAATGATTC
AAGCTATTAAGAATGGGGTCATTTTATGGCATGATGGACCAATGAATATG
AATTTCGAAAATATGAATGCTTTTGGATTAGAAGTATCTCTGAATATTTC
AGCTAATCTCAATAAACAATTCGGTTTTATAAAACCAGTTCGAGTTGTTT
CTTTGCGTGATGTCCCGGGTCTTAGTCGGGGGGTAATCAGAACTTTATTG
AAAAACAATGTAGTTGGAATCACGAGTGGAGTGAATTCAGGCTCAGCTCC
TCCTAATGTCACAAAGTTATGCAGATGGCGCGCTGCTCGACGTTCATTCA
AGGAACAGAATATTCCAGAGATTCTTTATATGCAAAATCCATGGGGTTAT
CCTGATGACGTTGGACCTAATCCTAATAATCCCGGAGGTATGAAAGAGCA
AGACTGTTTCCGAGCTCCAGGCTCAACTGAAGTGTTGTGCTTTAAATTTC
GTGTTGATAATACCGGTCCACCACAATCTATTCAGGAAGTATTAAACGCT
TATGAAGTTGTTAGAGGTCGTTACATTAATGCTAAATTATATGCTGGAAC
ATTTGAAGAATTTTTTGACATTGCTTGGAAAAATCGAAATTTATTACCAG
TAGTGGATAATGAAGTTGGTGACACTTGGATTCAAGGAACAGCTTCGGAT
CCCAAAAAGTTTGCTCGATTTCGAGCAATGCAAAGAGAATTCGAATTTTA
TTATCAAATGGGTAATCCTATAACTTCAAATGTTGTTGATGCATCTAGGT
TTTTGATTAAACCTGCCGAACATACTTATGGATTAGATAGTTGCGGTGAC
AGCATTAACTGGCGTAATGATCAATTTGAAAATATTAGACAACAACCCGG
AGTTCTCACTTCAGAATTATCGTGGATTGAGCAACGTCAATATCAACAAT
TGGTTTTAGATATTTTAGGCCCCACTCATCCATTAACAATATCTATTTTA
AAAGCCTTCAAACGATTGGAAGATTTTGATGGCATAGAATATCGAGGACA
TGGACAATCAGCCCGAAATTCAATTCACTCAAGTTTCAAGCTTCAAAATA
ACCCTTACTCTTTCGAATGCACAAACAACACACACTCGATTACGGTTGAA
TTTAAAGATGGAGCTATTACAAGCTTAAAAATGGATAATATTGAAATTAG
TCCGGAAAATCCTCTTGGAAAATTGATCTATAACACCTACAACGAATCTG
ATACAGAATTTTTCCTTAAATATTATAAAACATATTGGAACACTGGTTAT
GGAAAACCAAATTCAACAGTTAATGCTCACCCGGAAAGTAAAGAATGGAA
AATCGTAATGAAGAGTATTTATCGACAAAGTGATAGCCCATGTTCATTTT
TGTGCATACTCGAACCGGAAGATCCTAGAGCAACGTCATATTATGGTTTT
CCGGATTCCGTTTTGGTTTTATGGGATATTAATATCTCAACAAATGAAAT
TTCAACAGAGGTATTTTGGGATAATAAACCTCCAACTCGGCTTATGGAGG
CTATTTCAATCAAATTTCAACCGGAAATCGTTGGTAATCAATTTCAGTGG
TATTTGAGTAAAATGGGAGAACCGATATCTCCTTATTCTGTTATTAAAAA
TGGTAGTCAATGGCAGCATAATGTAGATGACTCTGGAGTGTTCATTTCTT
CAGCGCCAGATGCCAAACCGGTATTTCGAGTAATCCCATGGGATACTGGA
ATTACATGTCCGATAATCAAGGGCTATGGACCAACTGTATGGCCAGCTCC
TCTTTTCCCAATCACGAGCAATCTAACTGGAGTTGCATTTAATTTCTATA
ACAATGTGTGGAATACCAATTACATATATTGGTACCCGTTTATGGGGCCT
GGAACCCAATTGGACAATAGATTCAAGGCACGTTTTACAACTCAAGTGAA
TTTAGTGACCGAGAAAGTGGACGATACTCTTCGATATTACATTTAATTTT
GGAATTTGTGATGAATTATTTTTGTTATTCTTGACTTTTATAAATCCTCT
TATTGTGAACTCTAATTATTAATAAAAATATCAATTTGCTCAAAAAAAAA
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protein sequence of SMED30008076-orf-1

>SMED30008076-orf-1 ID=SMED30008076-orf-1|Name=SMED30008076-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=786bp
MIIVNSLFLLFVFYFSIIELSEGFGKSPPKNVKEDVIPEPDVTKVNIIFM
NHLDVGYSDHLAGLTGYTANVLNTYFNVHYPKAIDLANQLKSQGYVETFR
YTTHPWLLSFFLNCPKNLVLANIKVQCPNAAALNTMIQAIKNGVILWHDG
PMNMNFENMNAFGLEVSLNISANLNKQFGFIKPVRVVSLRDVPGLSRGVI
RTLLKNNVVGITSGVNSGSAPPNVTKLCRWRAARRSFKEQNIPEILYMQN
PWGYPDDVGPNPNNPGGMKEQDCFRAPGSTEVLCFKFRVDNTGPPQSIQE
VLNAYEVVRGRYINAKLYAGTFEEFFDIAWKNRNLLPVVDNEVGDTWIQG
TASDPKKFARFRAMQREFEFYYQMGNPITSNVVDASRFLIKPAEHTYGLD
SCGDSINWRNDQFENIRQQPGVLTSELSWIEQRQYQQLVLDILGPTHPLT
ISILKAFKRLEDFDGIEYRGHGQSARNSIHSSFKLQNNPYSFECTNNTHS
ITVEFKDGAITSLKMDNIEISPENPLGKLIYNTYNESDTEFFLKYYKTYW
NTGYGKPNSTVNAHPESKEWKIVMKSIYRQSDSPCSFLCILEPEDPRATS
YYGFPDSVLVLWDINISTNEISTEVFWDNKPPTRLMEAISIKFQPEIVGN
QFQWYLSKMGEPISPYSVIKNGSQWQHNVDDSGVFISSAPDAKPVFRVIP
WDTGITCPIIKGYGPTVWPAPLFPITSNLTGVAFNFYNNVWNTNYIYWYP
FMGPGTQLDNRFKARFTTQVNLVTEKVDDTLRYYI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0003824catalytic activity
GO:0016787hydrolase activity
Vocabulary: biological process
TermDefinition
GO:0006310DNA recombination
GO:0015074DNA integration
GO:0005975carbohydrate metabolic process
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002032epidermal cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR032482DUF5054
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032482Protein of unknown function DUF5054PFAMPF16477DUF5054coord: 352..614
e-value: 1.3E-16
score: 61.3
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..23
score: 0.811