CRAL-TRIO domain-containing protein

Overview
NameCRAL-TRIO domain-containing protein
Smed IDSMED30008074
Length (bp)1310
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of CRAL-TRIO domain-containing protein (SMED30008074) t-SNE clustered cells

Violin plots show distribution of expression levels for CRAL-TRIO domain-containing protein (SMED30008074) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CRAL-TRIO domain-containing protein (SMED30008074) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CRAL-TRIO domain-containing protein (SMED30008074) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008074

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30008074SMESG000009863.1 SmedASXL_013689SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30008074SMESG000009863.1 SmedASXL_013689SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30008074SMESG000009863.1 dd_Smed_v4_9151_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30008074SMESG000009863.1 dd_Smed_v4_9151_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30008074SMESG000009863.1 SMED_13183_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30008074SMESG000009863.1 SMED_13183_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30008074SMESG000009863.1 SmWIOct06_001714GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: TTPAL (alpha tocopherol transfer protein like [Source:HGNC Symbol;Acc:HGNC:16114])

HSP 1 Score: 77.411 bits (189), Expect = 4.844e-15
Identity = 57/211 (27.01%), Postives = 103/211 (48.82%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY + RRS PE      ++ L    + +V+    L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   80 FLLRFLRARKFDYDRALQLLVNYHSCRRSWPEV-----FNNLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGELD 282
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: TTPAL (alpha tocopherol transfer protein like [Source:HGNC Symbol;Acc:HGNC:16114])

HSP 1 Score: 77.411 bits (189), Expect = 4.844e-15
Identity = 57/211 (27.01%), Postives = 103/211 (48.82%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY + RRS PE      ++ L    + +V+    L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   80 FLLRFLRARKFDYDRALQLLVNYHSCRRSWPEV-----FNNLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGELD 282
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: TTPA (alpha tocopherol transfer protein [Source:HGNC Symbol;Acc:HGNC:12404])

HSP 1 Score: 68.9366 bits (167), Expect = 1.808e-12
Identity = 57/213 (26.76%), Postives = 96/213 (45.07%), Query Frame = 3 
Query:  411 VPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRAN-TKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQ 1043
            +P    FLLRFLR   +D  +A + L NY   R   PE S     + L    I  ++       L   D  G  V++ +   WDP      D+ RV  +  +LI  +  TQ +GI  I DL+    +   QI     + K    +  +++P++++ +    EP+IF    S+ +  +  K+K+R     N+ ++  S +     +LP EY G+
Sbjct:   45 LPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAECPEIS-----ADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDLEGWQFSHAFQI--TPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQ--SLLQHFPDILPLEYGGE 248
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: CLVS2 (clavesin 2 [Source:HGNC Symbol;Acc:HGNC:23046])

HSP 1 Score: 55.8398 bits (133), Expect = 6.471e-8
Identity = 56/262 (21.37%), Postives = 108/262 (41.22%), Query Frame = 3 
Query:  279 TN*KMSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLK-VPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGL-WDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            T+ +  +  ET    ++ELNE+ + +   ++       +   +  +  +  F+LRFLR  K+ H  A + L  Y   R+ + +  K F     ++  I + +       L   D     ILV F   WD +   L+D++R + + ++ +      Q +G  +I+D      K         L    + + ++++P R   + F  +P       ++    +K K +KR     N++  L   I     +LP E+ G   P D
Sbjct:    2 THLQAGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFHHFEAFRLLAQYFEYRQQNLDMFKSF---KATDPGIKQALKDGFPGGLANLDHYGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLTPSMLRLAIEGL-QDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHP--EILPSEFGGMLPPYD 257
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: RLBP1 (retinaldehyde binding protein 1 [Source:HGNC Symbol;Acc:HGNC:10024])

HSP 1 Score: 53.5286 bits (127), Expect = 3.254e-7
Identity = 48/247 (19.43%), Postives = 100/247 (40.49%), Query Frame = 3 
Query:  303 SETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNG 1040
            +E E   +  + E  E + A   S  +   +       ++  F LRF+R  K++   A + L  Y   R  +PE      + +LS E +   I+      L   D  G++V+L     W    +   ++++     ++ +     TQ +G  +I +      +        +L +    + ++++P R K + F  +P  F    ++ +  +KSK+ +R     +D+      I     +LP ++ G
Sbjct:   53 NEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRLQYPEL-----FDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDL-RKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEID--ENILPSDFGG 291
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG33965 (gene:FBgn0053965 transcript:FBtr0100006)

HSP 1 Score: 85.8853 bits (211), Expect = 3.056e-18
Identity = 69/247 (27.94%), Postives = 113/247 (45.75%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLL--VPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFM-GIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELL--MKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPL 1055
            ELNE    +++ + +  +W     HL      +FLL FLR +K+    A++++D + +L+   PE   F D   + N  + E+I L ++  +PL   D G  V +++ G +D N  K  D++RV  M G  ++         G   IMD+    T  N    Q +L   +    +E  P R K + F   P  F+       LL     K+K+R + + +D E +  R+P     LP EY G  G +
Sbjct:   23 ELNEVPSRVESDIAALKEWLQKQPHLCACLEDQFLLSFLRGSKFSLEKAKQKIDRFYSLQAVIPEV--FNDQRLVDNAQVLEIIRLGVILRIPLDEEDTGPAVTIIRAGSYDINKFKFQDIIRVGSMFGEIMMLEDDNASVSGYLEIMDMSGV-TGANLFALQPQLLSKFSAYADEAMPTRQKGIHFINVPKAFE--TGFKSLLGWFPGKIKER-VSVSSDPEAIFERVP--KHYLPEEYGGSKGTM 261
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG12926 (gene:FBgn0033437 transcript:FBtr0088502)

HSP 1 Score: 81.2629 bits (199), Expect = 8.605e-17
Identity = 66/259 (25.48%), Postives = 111/259 (42.86%), Query Frame = 3 
Query:  294 SIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDE----VIDLNLL--VPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRV-LFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSG 1049
            S+  E       EL E  + ID  +E+   W S   HLK  ++ +FL+ FLR  KY     + +LDN+  +R + PE  K        N I+ E    ++D   L  +P     DG  + + ++G +D     + ++++V   +G   I         G   I+D+       +       L K    + ++ YP R K   F   P   +   SI++ LM  K++KR   + + ++ L   +P     LP EY G +G
Sbjct:   11 SLSPELAKKAHDELGEIPDRIDEDIETLRTWISKQPHLKARQDAQFLVAFLRGCKYSLEKTKLKLDNFYAMRGAVPELYK--------NRIVGEKQLSILDTGCLLRLPQPLQADGPRIHISRYGQYDSKKYSIAEVVQVNTMLGEIQIREDDNAMISGFVEIIDMKGVGAG-HLFQFDAVLVKKLAVLGDKAYPYRPKGFHFVNAPSSAEKFMSIAKSLMSEKIRKR-FHIHSKLDSLYKYVP--KECLPAEYGGSNG 257
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG31636 (gene:FBgn0051636 transcript:FBtr0079324)

HSP 1 Score: 79.337 bits (194), Expect = 4.400e-16
Identity = 62/246 (25.20%), Postives = 114/246 (46.34%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLL--VPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQT-HGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPL 1055
            ELNE    +   +E+   W     HL+   + +FLL FLR  K+    A+++ D + TL+RS PE   F +    ++  + +++ + +L  +P+   D G  V +++ G +D +  K  D++RV  M  +++  +    T  G   IMD+  A    + ++  Q +L   +    +E  P R K + F   P  F+   +       +K+K R + + +D   +   +    + LP EY G  G L
Sbjct:   18 ELNELPARMAQDIEALRDWVLKQPHLRACTDDQFLLAFLRGTKFSLERAKEKFDRFYTLQRSIPEV--FNERRLATDPQVLDIVRMGVLLQIPMDADDPGPRVTIIRAGSYDTSKHKFQDIIRVGSMFGEIMMFEDDNATVSGYVEIMDM--AGVTGSHLFALQPQLLSKFSTYADEAMPTRQKGIHFINVPAAFETGFNSLRSFFPAKIKSR-ISVSSDPAAIYELV--RRKYLPQEYGGTGGNL 256
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG31636 (gene:FBgn0051636 transcript:FBtr0343865)

HSP 1 Score: 79.337 bits (194), Expect = 4.400e-16
Identity = 62/246 (25.20%), Postives = 114/246 (46.34%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLL--VPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQT-HGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPL 1055
            ELNE    +   +E+   W     HL+   + +FLL FLR  K+    A+++ D + TL+RS PE   F +    ++  + +++ + +L  +P+   D G  V +++ G +D +  K  D++RV  M  +++  +    T  G   IMD+  A    + ++  Q +L   +    +E  P R K + F   P  F+   +       +K+K R + + +D   +   +    + LP EY G  G L
Sbjct:   18 ELNELPARMAQDIEALRDWVLKQPHLRACTDDQFLLAFLRGTKFSLERAKEKFDRFYTLQRSIPEV--FNERRLATDPQVLDIVRMGVLLQIPMDADDPGPRVTIIRAGSYDTSKHKFQDIIRVGSMFGEIMMFEDDNATVSGYVEIMDM--AGVTGSHLFALQPQLLSKFSTYADEAMPTRQKGIHFINVPAAFETGFNSLRSFFPAKIKSR-ISVSSDPAAIYELV--RRKYLPQEYGGTGGNL 256
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG33514 (gene:FBgn0053514 transcript:FBtr0091456)

HSP 1 Score: 78.9518 bits (193), Expect = 5.971e-16
Identity = 61/243 (25.10%), Postives = 111/243 (45.68%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLV--PLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVL-FMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSG 1049
            +L E  E ++A +++F  W     HL    + +FL+ FLR  KY    A+ +LD Y TL+  +P+   +F  +  ++    E+     ++  P   +++G  + + + GL       +++ M+V   M    I        +G+  IMD+  A T  +       + K +    EE  P+RLK   F      F+   ++ + +M  KM+ R     N +  L  +IP  ++ LP EY G++G
Sbjct:   19 QLKEDPERLEADLQAFKTWIEQQPHLNPRMDDQFLVAFLRGCKYSLERAKSKLDKYYTLKTKYPD---YFRVTNTTDSKFREIHQTGAIIYLPTPLNENGPRIGIWRMGLVPVEKYTMLECMQVAQAMQEIAILEDDYANVNGVVFIMDMKGA-TAAHLFQMTPSMAKKFTVFSEEALPLRLKAQHFINTITGFEQLFNMFKPMMSKKMQSRLFVHGNKMGLLTEQIP--LKYLPEEYGGENG 255
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:ZFIN;Acc:ZDB-GENE-030131-296])

HSP 1 Score: 97.0561 bits (240), Expect = 2.468e-22
Identity = 66/215 (30.70%), Postives = 108/215 (50.23%), Query Frame = 3 
Query:  420 NKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELF-KNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            +  FLLRFLR  K+D+  A + L NY   RR+ PE      +  L    +  V+DL  L  L   D  G+ ++ ++ G W PN    +D +R +++ ++ +     TQ +G+ +++D   A   ++Q  N   L  K    I ++ +P+R+K +    EP IF    +I +  +K KM +R +   +D+  L   IP    +LP EY G SG LD
Sbjct:   14 DDAFLLRFLRARKFDYDRALQLLLNYHASRRAWPEV-----FQDLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYPFVDNIRAIYLTLEKLIQPEETQVNGVVILVDY--AGVGLSQASNPGPLLAKKVVGILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLASLHRVIP--QSVLPQEYGGVSGRLD 219
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:ZFIN;Acc:ZDB-GENE-030131-296])

HSP 1 Score: 97.0561 bits (240), Expect = 6.828e-22
Identity = 66/212 (31.13%), Postives = 107/212 (50.47%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELF-KNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY   RR+ PE      +  L    +  V+DL  L  L   D  G+ ++ ++ G W PN    +D +R +++ ++ +     TQ +G+ +++D   A   ++Q  N   L  K    I ++ +P+R+K +    EP IF    +I +  +K KM +R +   +D+  L   IP    +LP EY G SG LD
Sbjct:   79 FLLRFLRARKFDYDRALQLLLNYHASRRAWPEV-----FQDLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYPFVDNIRAIYLTLEKLIQPEETQVNGVVILVDY--AGVGLSQASNPGPLLAKKVVGILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLASLHRVIP--QSVLPQEYGGVSGRLD 281
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: rlbp1b (retinaldehyde binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1870])

HSP 1 Score: 65.4698 bits (158), Expect = 2.130e-11
Identity = 49/211 (23.22%), Postives = 93/211 (44.08%), Query Frame = 3 
Query:  432 LLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDA 1061
            L+RF+R  KYD + A + +  Y   RR +PE      +  L+ E +   I+      L   D  G++V+L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ + LMKSK+ +R     +D+E           +LP +++G+    D 
Sbjct:   96 LVRFIRARKYDVNRAYELMKGYVRFRRDYPEL-----FENLTPEAVRSTIEAGYPGILSSRDKYGRVVLLFNIENWDYEEITFDEILRAYCVILEKLLENEETQINGFCIIENFKGFTMQQASGIKPTEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPLMKSKLLERVFVHGDDLENYFKEFDA--EILPSDFDGKGSKYDG 298
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: rlbp1b (retinaldehyde binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1870])

HSP 1 Score: 65.4698 bits (158), Expect = 2.130e-11
Identity = 49/211 (23.22%), Postives = 93/211 (44.08%), Query Frame = 3 
Query:  432 LLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDA 1061
            L+RF+R  KYD + A + +  Y   RR +PE      +  L+ E +   I+      L   D  G++V+L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ + LMKSK+ +R     +D+E           +LP +++G+    D 
Sbjct:   96 LVRFIRARKYDVNRAYELMKGYVRFRRDYPEL-----FENLTPEAVRSTIEAGYPGILSSRDKYGRVVLLFNIENWDYEEITFDEILRAYCVILEKLLENEETQINGFCIIENFKGFTMQQASGIKPTEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPLMKSKLLERVFVHGDDLENYFKEFDA--EILPSDFDGKGSKYDG 298
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: rlbp1b (retinaldehyde binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1870])

HSP 1 Score: 65.4698 bits (158), Expect = 2.130e-11
Identity = 49/211 (23.22%), Postives = 93/211 (44.08%), Query Frame = 3 
Query:  432 LLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDA 1061
            L+RF+R  KYD + A + +  Y   RR +PE      +  L+ E +   I+      L   D  G++V+L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ + LMKSK+ +R     +D+E           +LP +++G+    D 
Sbjct:   96 LVRFIRARKYDVNRAYELMKGYVRFRRDYPEL-----FENLTPEAVRSTIEAGYPGILSSRDKYGRVVLLFNIENWDYEEITFDEILRAYCVILEKLLENEETQINGFCIIENFKGFTMQQASGIKPTEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPLMKSKLLERVFVHGDDLENYFKEFDA--EILPSDFDGKGSKYDG 298
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:NCBI gene;Acc:548930])

HSP 1 Score: 90.8929 bits (224), Expect = 1.196e-19
Identity = 61/220 (27.73%), Postives = 109/220 (49.55%), Query Frame = 3 
Query:  402 HLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            HLK   +  FLLRFLR  K+D+  A + L NY + R++ PE      ++ L    +  V+D   L  L  +D +G+ ++ ++ G W P    + + +R +++ ++ +     TQ +GI ++ D +         +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+ KR     +D+  L + IP    +LP EY G +G LD
Sbjct:   68 HLKTRVDDSFLLRFLRARKFDYDRALQLLVNYYSCRKAWPEV-----FTDLRPSAVKPVLDSGFLTVLPHTDTEGRRIVCIRPGCWIPRDYPITENIRAIYLSLEKLVESEETQVNGIVILADYNGVGLTHASHFGPF-IAKKVIGILQDGFPIRIKAVNVINEPRIFKGIFAILKPFLKEKIVKRFFLHGSDLNSLHANIP--KSILPEEYGGTAGKLD 279
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: rlbp1 (retinaldehyde binding protein 1 [Source:NCBI gene;Acc:448050])

HSP 1 Score: 65.4698 bits (158), Expect = 3.754e-11
Identity = 52/247 (21.05%), Postives = 105/247 (42.51%), Query Frame = 3 
Query:  303 SETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNG 1040
            +ETE   +  + E    +     +  + C +       +  +F LRF+R  K+D S A + L  Y   R+ +PE      +  L+ E +   I+      L   D +G++++L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ +  +KSK+ +R     +D+E     I     +LP ++ G
Sbjct:   59 NETEEKRESAVKELRALVQEKANAGDELCKAVADKVKDKGDDFFLRFIRARKFDVSRAYELLKGYVNFRQQYPEL-----FEDLTPEAVRSTIEAGYPGILTSRDKNGRVILLFNIESWDYEEITFDEILRAYCIILESLLENEETQINGFCIIENFKGFTMQQASGIKPSEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLEGFYKEIDA--DILPADFGG 297
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: rlbp1 (retinaldehyde binding protein 1 [Source:NCBI gene;Acc:448050])

HSP 1 Score: 65.0846 bits (157), Expect = 4.727e-11
Identity = 52/247 (21.05%), Postives = 105/247 (42.51%), Query Frame = 3 
Query:  303 SETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNG 1040
            +ETE   +  + E    +     +  + C +       +  +F LRF+R  K+D S A + L  Y   R+ +PE      +  L+ E +   I+      L   D +G++++L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ +  +KSK+ +R     +D+E     I     +LP ++ G
Sbjct:   62 NETEEKRESAVKELRALVQEKANAGDELCKAVADKVKDKGDDFFLRFIRARKFDVSRAYELLKGYVNFRQQYPEL-----FEDLTPEAVRSTIEAGYPGILTSRDKNGRVILLFNIESWDYEEITFDEILRAYCIILESLLENEETQINGFCIIENFKGFTMQQASGIKPSEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLEGFYKEIDA--DILPADFGG 300
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: ggh (gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) [Source:NCBI gene;Acc:594986])

HSP 1 Score: 63.5438 bits (153), Expect = 1.143e-10
Identity = 58/220 (26.36%), Postives = 94/220 (42.73%), Query Frame = 3 
Query:  420 NKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELF-------KNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRT-LRLKNDIEKLDSRIPGLMRLLPFEYNGQSGP 1052
            + +FLLRFLR   +   +A K L NY   R   PE +     + L    I ++        L   DD G  V++ +   WDP      ++ RV  +  +LI  +  TQ +GI  I DL        Q W     F       K    +  +++P++++ +    EP+ F    +I +  +  K+K+R  +   N I  L+        +LP EY G +GP
Sbjct:   48 SDDFLLRFLRARDFCIELAFKLLKNYHKWRAECPEIT-----ADLRPSPILDLFRAGYHAVLRSRDDSGSKVLIYRIEYWDPKLFTAYEVFRVSLITSELIVQEAETQRNGIKAIFDL--------QGWRLAHAFQITPTMAKRIAAVMADSFPLKVRGVHLINEPLFFHPVFAIIKPFLPDKIKQRVHMHGSNYIPTLNQHFS--TSILPPEYGG-TGP 251
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: thg1l (tRNA-histidine guanylyltransferase 1-like [Source:Xenbase;Acc:XB-GENE-966760])

HSP 1 Score: 54.299 bits (129), Expect = 1.618e-7
Identity = 56/262 (21.37%), Postives = 109/262 (41.60%), Query Frame = 3 
Query:  279 TN*KMSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLK-VPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGL-WDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            T+ +  +  ET    ++ELNE+ + +   ++       +   +  +  +  F+LRFLR  K++H  A + L  Y   R+ + +  K F     ++  I + +       L   D     ILV F   WD +   L+D++R + + ++ +      Q +G  +I+D      K         L    + + ++++P R   + F  +P       ++    +K K +KR     N++  L   I     +LP E+ G   P D
Sbjct:    2 THLQAGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYRQQNLDMFKNF---KATDPGIKQGLKDGFPGVLSNLDHFGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLTPSMLRLAIEGL-QDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHP--EILPSEFGGMLPPYD 257
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpal (tocopherol (alpha) transfer protein-like [Source:MGI Symbol;Acc:MGI:1923330])

HSP 1 Score: 78.5666 bits (192), Expect = 1.432e-15
Identity = 59/220 (26.82%), Postives = 106/220 (48.18%), Query Frame = 3 
Query:  402 HLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            +L    +  FLLRFLR  K+D+  A + L NY   RRS PE      +S L    + +V++   L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   72 YLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHGCRRSWPEV-----FSNLRPSALKDVLNSGFLTVLPHTDPRGCHVLCIRPDRWIPSNYPITENIRAVYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHIVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RNILPKEYGGTAGELD 283
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpal (tocopherol (alpha) transfer protein-like [Source:MGI Symbol;Acc:MGI:1923330])

HSP 1 Score: 78.5666 bits (192), Expect = 1.432e-15
Identity = 59/220 (26.82%), Postives = 106/220 (48.18%), Query Frame = 3 
Query:  402 HLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            +L    +  FLLRFLR  K+D+  A + L NY   RRS PE      +S L    + +V++   L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   72 YLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHGCRRSWPEV-----FSNLRPSALKDVLNSGFLTVLPHTDPRGCHVLCIRPDRWIPSNYPITENIRAVYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHIVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RNILPKEYGGTAGELD 283
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpa (tocopherol (alpha) transfer protein [Source:MGI Symbol;Acc:MGI:1354168])

HSP 1 Score: 65.855 bits (159), Expect = 1.608e-11
Identity = 57/242 (23.55%), Postives = 104/242 (42.98%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPE--NKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQ 1043
            EL +HS  +   +    +        + P+     FLLRFLR   +D  +A + + NY   R   PE S     + L    I  ++       L   D  G  V++ +   WDP      D+ RV  +  +LI  +  TQ +G+  I DL+    +++  +     + K    +  +++P++++ +    EP+IF    S+ +  +  K+K R     N+ +   S +     +LP EY G+
Sbjct:   16 ELPDHSPLLQPGLAELRRRVQEAGVPQTPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAECPELS-----ADLRPRSILGLLKAGYHGVLRSRDSTGSRVLIYRIAYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDLE--GWQVSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKDRIHLHGNNYK--SSMLQHFPDILPREYGGK 248
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Ttpa (tocopherol (alpha) transfer protein [Source:MGI Symbol;Acc:MGI:1354168])

HSP 1 Score: 62.3882 bits (150), Expect = 1.907e-10
Identity = 50/213 (23.47%), Postives = 92/213 (43.19%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPE--NKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKR 956
            EL +HS  +   +    +        + P+     FLLRFLR   +D  +A + + NY   R   PE S     + L    I  ++       L   D  G  V++ +   WDP      D+ RV  +  +LI  +  TQ +G+  I DL+    +++  +     + K    +  +++P++++ +    EP+IF    S+ +  +  K+K R
Sbjct:   16 ELPDHSPLLQPGLAELRRRVQEAGVPQTPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAECPELS-----ADLRPRSILGLLKAGYHGVLRSRDSTGSRVLIYRIAYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDLE--GWQVSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKDR 221
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Clvs2 (clavesin 2 [Source:MGI Symbol;Acc:MGI:2443223])

HSP 1 Score: 56.6102 bits (135), Expect = 2.367e-8
Identity = 57/266 (21.43%), Postives = 110/266 (41.35%), Query Frame = 3 
Query:  279 TN*KMSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLK-VPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGL-WDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKI----WEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            T+ +  +  ET    ++ELNE+ + +   ++       +   +  +  +  F+LRFLR  K+ H  A + L  Y   R+ + +  K F     ++  I + +       L   D     ILV F   WD +   L+D++R + + ++ +      Q +G  +I+D      K        +L  N  ++     ++++P R   + F  +P       ++    +K K +KR     N++  L   I     +LP E+ G   P D
Sbjct:    2 THLQAGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFHHFEAFRLLAQYFEYRQQNLDMFKSF---KATDPGIKQALKDGFPGGLANLDHYGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFK-----QASKLTPNMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHP--EILPSEFGGMLPPYD 257
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9D3D0|TTPAL_MOUSE (Alpha-tocopherol transfer protein-like OS=Mus musculus OX=10090 GN=Ttpal PE=1 SV=3)

HSP 1 Score: 78.5666 bits (192), Expect = 1.002e-14
Identity = 59/220 (26.82%), Postives = 106/220 (48.18%), Query Frame = 3 
Query:  402 HLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            +L    +  FLLRFLR  K+D+  A + L NY   RRS PE      +S L    + +V++   L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   72 YLSTSLDDAFLLRFLRARKFDYDRALQLLVNYHGCRRSWPEV-----FSNLRPSALKDVLNSGFLTVLPHTDPRGCHVLCIRPDRWIPSNYPITENIRAVYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHIVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RNILPKEYGGTAGELD 283
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9BTX7|TTPAL_HUMAN (Alpha-tocopherol transfer protein-like OS=Homo sapiens OX=9606 GN=TTPAL PE=1 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 2.326e-14
Identity = 57/211 (27.01%), Postives = 103/211 (48.82%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY + RRS PE      ++ L    + +V+    L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   80 FLLRFLRARKFDYDRALQLLVNYHSCRRSWPEV-----FNNLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGELD 282
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5RFR0|TTPAL_PONAB (Alpha-tocopherol transfer protein-like OS=Pongo abelii OX=9601 GN=TTPAL PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.459e-14
Identity = 57/211 (27.01%), Postives = 103/211 (48.82%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY + RRS PE      ++ L    + +V+    L  L  +D  G  V+ ++   W P++  + + +R +++ ++ +     TQ +GI ++ D    +      +    + K    I ++ +P+R+K +    EP IF    +I +  +K K+  R     +D+  L + +P    +LP EY G +G LD
Sbjct:   80 FLLRFLRARKFDYDRALQLLVNYHSCRRSWPEV-----FNNLKPSALKDVLASGFLTVLPHTDPRGCHVVCIRPDRWIPSNYPITENIRAIYLTLEKLIQSEETQVNGIVILADYKGVSLSKASHFGPF-IAKKVIGILQDGFPIRIKAVHVVNEPRIFKGIFAIIKPFLKEKIANRFFLHGSDLNSLHTNLP--RSILPKEYGGTAGELD 282
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|P49638|TTPA_HUMAN (Alpha-tocopherol transfer protein OS=Homo sapiens OX=9606 GN=TTPA PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.683e-12
Identity = 57/213 (26.76%), Postives = 96/213 (45.07%), Query Frame = 3 
Query:  411 VPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRAN-TKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQ 1043
            +P    FLLRFLR   +D  +A + L NY   R   PE S     + L    I  ++       L   D  G  V++ +   WDP      D+ RV  +  +LI  +  TQ +GI  I DL+    +   QI     + K    +  +++P++++ +    EP+IF    S+ +  +  K+K+R     N+ ++  S +     +LP EY G+
Sbjct:   45 LPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAECPEIS-----ADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDLEGWQFSHAFQI--TPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQ--SLLQHFPDILPLEYGGE 248
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|P41034|TTPA_RAT (Alpha-tocopherol transfer protein OS=Rattus norvegicus OX=10116 GN=Ttpa PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 5.512e-11
Identity = 64/270 (23.70%), Postives = 116/270 (42.96%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKE-----FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDALRSILNDF--KLENFINLI 1112
            +LNE  ++        A+         VPE  +     FLLRFLR   +D  +A + + NY   R   PE S     + L    I  ++       L   D  G  V++ +   WDP      D+ RV  +  +LI  +  TQ +G+  I DL+    +I+  +     + K    +  +++P++++ +    EP+IF    S+ +  +  K+K R     N+ +   S +     +LP EY G    ++ +     +F  K E++++ I
Sbjct:   13 QLNEQPDHSPLVQPGLAELRRRAQEEGVPETPQPLTDAFLLRFLRARDFDLDLAWRLMKNYYKWRAECPELS-----ADLHPRSILGLLKAGYHGVLRSRDPTGSRVLIYRISYWDPKVFTAYDVFRVSLITSELIVQEVETQRNGVKAIFDLE--GWQISHAFQITPSVAKKIAAVVTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKGRIHLHGNNYK--SSLLQHFPDILPLEYGGNESSMEDICQEWTNFIMKSEDYLSSI 273
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A5J4P3R6 (CRAL-TRIO domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0006189 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 7.957e-50
Identity = 82/242 (33.88%), Postives = 150/242 (61.98%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPL 1055
            E+ E  +++ AH+ SF +W +S+ HLK+P+N  FLL FLR A Y+H++AQ+R+DN+CTLR S    S+++D+ T+ + ++D  ++  + +PLG  + G  V+L++   W P  +  +++  +  M  + +      Q  G  +++D+  ++ +  + W   +  K+  K+ +E  P R+K+L+F++E  IFD    +SEL M  KM+ R +R+K++I+K   ++PGL  +LP EY G++G L
Sbjct:   20 EVGEKYDHVGAHLISFKRWLASSPHLKLPQNDVFLLSFLRYAHYEHALAQRRIDNFCTLRCSDKLISQWYDYPTVEDPLLDTYLNAGIFIPLGFLESGLYVVLIRMASWSPTEISQVEMRTLNNMNFERLLFDQRCQIGGFVLMIDMAHSSLETARQWGDIKYAKSAFKLMQEASPARVKHLVFYKESKIFDFAFKLSELWMSDKMRGRVIRVKDNIQKAFKKVPGLKEILPEEYEGKNGTL 261
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A4S2LBZ0 (CRAL-TRIO domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009855 PE=4 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 5.231e-49
Identity = 86/250 (34.40%), Postives = 150/250 (60.00%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDALRSILN 1079
            E+ E++E++ AH+ SF +W +++ HLK+P+   FLL FLR A YDH++AQ+R+DN+CTLR S    S ++++  L +  +++ ++  + VPLG  D+G  VIL++   W P  +  + +  +  M  + +      Q  G T+++D+     +    W   +  K+  K+ +E+ P R+KNLIF++E  +FD    ++E+ +  KM+ R  R+K+DI K   +IPGL  +LP EY+G++G L+   S  N
Sbjct:   35 EIGENAEHVMAHLTSFKRWLAASPHLKIPQVDTFLLSFLRYAHYDHALAQRRIDNFCTLRYSDKHISPWYEYPKLDDPRLEKYLNAGIFVPLGFMDNGMYVILIRLASWSPAELDQVSMRAMNNMNFERLLFDQRCQIGGFTLLIDMAHTTMEQATQWGDFKHAKSAFKLMQESSPGRVKNLIFYKETKLFDFAFKLAEVWLSEKMRNRVTRVKDDINKAFKKIPGLKDVLPTEYHGKNGTLEEALSRCN 284
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A1S8X0L3 (CRAL-TRIO domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=X801_04070 PE=4 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 3.177e-48
Identity = 85/249 (34.14%), Postives = 150/249 (60.24%), Query Frame = 3 
Query:  312 ENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            +N  + E+ E++E++ AH+ SF +W +++ HLK+P+   FLL FLR A YDH++AQ+R+DN+CTLR S    S ++++  L +  +++ ++  + VPLG  D+G  VIL++   W P  +  + +  +  M  + +      Q  G T+ +D+     +    W   +  K+  K+ +E+ P R+KNLIF++E  +FD    ++E+ +  KM+ R  R+K+DI K   +IPGL  +LP EY+G++G L+
Sbjct:   70 QNLAKKEVGENAEHVMAHLTSFKRWLAASPHLKIPQVDTFLLSFLRYAHYDHALAQRRIDNFCTLRYSDKHISPWYEYPKLDDPRLEKYLNAGIFVPLGFMDNGMYVILIRLASWSPAELDQVAMRAMNNMNFERLLFDQRCQIGGFTLFIDMAHTTMEQATQWGDFKHAKSAFKLMQESSPGRVKNLIFYKETKLFDFAFKLAEVWLSEKMRNRVTRVKDDINKAFKKIPGLKDVLPMEYHGKNGTLE 318
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A4S2LBM1 (CRAL-TRIO domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009855 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 3.765e-48
Identity = 86/253 (33.99%), Postives = 153/253 (60.47%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKE---FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDALRSILN 1079
            E+ E++E++ AH+ SF +W +++ HLK+P++++   FLL FLR A YDH++AQ+R+DN+CTLR S    S ++++  L +  +++ ++  + VPLG  D+G  VIL++   W P  +  + +  +  M  + +      Q  G T+++D+     +    W   +  K+  K+ +E+ P R+KNLIF++E  +FD    ++E+ +  KM+ R  R+K+DI K   +IPGL  +LP EY+G++G L+   S  N
Sbjct:   35 EIGENAEHVMAHLTSFKRWLAASPHLKIPQDQDLDTFLLSFLRYAHYDHALAQRRIDNFCTLRYSDKHISPWYEYPKLDDPRLEKYLNAGIFVPLGFMDNGMYVILIRLASWSPAELDQVSMRAMNNMNFERLLFDQRCQIGGFTLLIDMAHTTMEQATQWGDFKHAKSAFKLMQESSPGRVKNLIFYKETKLFDFAFKLAEVWLSEKMRNRVTRVKDDINKAFKKIPGLKDVLPTEYHGKNGTLEEALSRCN 287
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A267E9H4 (CRAL-TRIO domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023111g1 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 9.668e-48
Identity = 92/248 (37.10%), Postives = 138/248 (55.65%), Query Frame = 3 
Query:  324 QVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRI-PGLMRLLPFEYNGQSGPLDAL 1064
            Q EL+E+ + + AH+ S  +W +S  HL  P +  FLLRFLRTAK+D S AQ+ +DN+CT+R+S     ++F+   L + II+E + L + + LG +DDG+ +I      +DP+ +   DL R  +M  D +      Q +G   IM+L  AN K  Q W          + W++ +PMR K L+++ EP  F++   I    MK K++KR  R   D  KL   I PG  RLLP E  GQ+ P++ L
Sbjct:   24 QKELHENPDQVRAHIASLRRWVTSAPHLTCPTDDAFLLRFLRTAKFDQSRAQQLVDNFCTVRKSPRGSPEWFEPLPLDSPIIEEALKLPMNIFLGYNDDGEGIIYSSMDNYDPDRINFNDLARFAYMNADTMMKPEKIQINGCRFIMNLKNANKKQTQAWENPRTAFKMMRNWQDAFPMRTKALVYWHEPAFFELMFRIIMPFMKEKLRKRLHRCGTDFNKLKKAIGPGAERLLPPEMGGQNRPMEEL 271
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ttpal (alpha tocopherol transfer protein like [Source:NCBI gene;Acc:103043466])

HSP 1 Score: 94.7449 bits (234), Expect = 3.970e-21
Identity = 65/212 (30.66%), Postives = 106/212 (50.00%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELF-KNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY   RR+ PE      +  L    +  V+DL  L  L   D  G+ ++ ++ G W PN    +D +R +++ ++ +     TQ +GI +++D       ++Q  N   L  K    I ++ +P+R+K +    EP IF    +I +  +K KM +R +   +D+  L   IP    +LP EY G +G LD
Sbjct:   82 FLLRFLRARKFDYDRAMQLLLNYHGSRRAWPEV-----FQDLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYHFVDNIRAIYLTLEKLIQPEETQVNGIVILVDY--TGVGLSQASNPGPLLAKKVVSILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLSSLHRVIP--RSVLPEEYGGVAGRLD 284
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ttpal (alpha tocopherol transfer protein like [Source:NCBI gene;Acc:103043466])

HSP 1 Score: 93.9745 bits (232), Expect = 7.634e-21
Identity = 65/212 (30.66%), Postives = 106/212 (50.00%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELF-KNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            FLLRFLR  K+D+  A + L NY   RR+ PE      +  L    +  V+DL  L  L   D  G+ ++ ++ G W PN    +D +R +++ ++ +     TQ +GI +++D       ++Q  N   L  K    I ++ +P+R+K +    EP IF    +I +  +K KM +R +   +D+  L   IP    +LP EY G +G LD
Sbjct:   83 FLLRFLRARKFDYDRAMQLLLNYHGSRRAWPEV-----FQDLKPSTVKHVLDLGFLTVLPRPDPQGRYILCLRPGKWKPNDYHFVDNIRAIYLTLEKLIQPEETQVNGIVILVDY--TGVGLSQASNPGPLLAKKVVSILQDGFPIRIKAVNIINEPRIFKGIFAIIKPFLKEKMAERYVLHGSDLSSLHRVIP--RSVLPEEYGGVAGRLD 285
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: rlbp1b (retinaldehyde-binding protein 1 [Source:NCBI gene;Acc:103021449])

HSP 1 Score: 65.4698 bits (158), Expect = 1.725e-11
Identity = 56/235 (23.83%), Postives = 103/235 (43.83%), Query Frame = 3 
Query:  360 AHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDA 1061
            A  +  AK   ST   K P++   L+RF+R  KY+ + A + +  Y   RR +PE      +  L+ E +   I+      L   D  G++V+L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ + LMKSK+ +R     +++E           +LP E+NG+    D 
Sbjct:   75 ASGDELAKAVQSTFGEK-PDD--VLVRFIRARKYEVNRAFELMKGYVRFRRDYPEL-----FENLTPEAVRSTIEAGYPGILSSRDKYGRVVLLFNIENWDYEEITFDEILRAYCVILEKLLENEETQINGFCIIENFKGFTMQQASGIKPTEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPLMKSKLLERVFVHGDELESYYKEFDP--EILPSEFNGKGSKYDG 298
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ttpa (tocopherol (alpha) transfer protein [Source:ZFIN;Acc:ZDB-GENE-030131-4631])

HSP 1 Score: 58.5362 bits (140), Expect = 6.723e-9
Identity = 54/217 (24.88%), Postives = 91/217 (41.94%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELF-------KNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRT-LRLKNDIEKLDSRIPGLMRLLPFEYNGQSGP 1052
            FLL+FLR   +D  +A K L NY   R+  PE S     + L    +  ++  N    L   DD G  +++ +   W+P      ++ RV  +  +LI  +  TQ +G+  I DL        Q W     F       K    +  +++P++++ +    EPI F     +    +  K+K+R  +   +    L    P    + P EY G +GP
Sbjct:   48 FLLKFLRARDFDLDLALKLLINYHKWRQECPEIS-----ANLQPSSVIGLLKNNYHGVLKSRDDAGSRILIYRISQWNPKEFSAYEVFRVSLITSELIVQERDTQINGLKAIFDL--------QGWCFAHAFQINPFLAKKISSVLTDSFPLKVRGIHLINEPIFFRPVFKMIRPFLPDKIKQRVHIHGSSYTASLYEHFP--KAIFPPEYGG-TGP 248
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: rlbp1a (retinaldehyde-binding protein 1-like [Source:NCBI gene;Acc:103033538])

HSP 1 Score: 56.9954 bits (136), Expect = 1.565e-8
Identity = 49/224 (21.88%), Postives = 94/224 (41.96%), Query Frame = 3 
Query:  429 FLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDALRSILNDFKLEN 1097
             LLRF+R  K+D + A + +  Y   RR +PE      +  ++ E +   I+      L   D  G++VIL     WD   V   + +R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K +    +P  F    ++ +  MKSK+ +R     +++E           +LP +++G     D   + +  F  E+
Sbjct:  175 LLLRFIRARKFDVTRAHELMKGYVRFRRDYPEL-----FENMTPEAVRSTIEAGYPRILSTRDKYGRVVILFNIENWDLEEVTFDETLRAYCVILEKLLENEETQINGFVLIENFKGFTMQHASGIKHTEL-KKMVDMLQDSFPARFKAVHVIHQPWYFTTTYNVVKPFMKSKLLERVHVHGDELETYLRDFGA--EILPPDFDGTGPNCDGKETAIKLFGSED 390
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ttpal (tocopherol (alpha) transfer protein-like [Source:ZFIN;Acc:ZDB-GENE-030131-296])

HSP 1 Score: 82.0333 bits (201), Expect = 5.061e-18
Identity = 56/223 (25.11%), Postives = 105/223 (47.09%), Query Frame = 3 
Query:  294 SIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKR 956
            S+  E     +VEL E+ E     V+   +  +    +    +  FLLRFLR  K+DH    K +++Y     + P+  + F  S      +  ++    +  L   D  G+ VI+   G W+P++  ++D +R ++M ++L+     TQ +GIT++   D    ++  + N    L K    + ++ +P+R+K      EP IF    ++ +  +K KM+KR
Sbjct:   33 SLSPELVERARVELGENPETRAQEVQKLREALARRPDIPARTDDAFLLRFLRARKFDHEKTFKLVESYYKCHETWPDIFQNFRPSA-----VKALLGSGFIRVLPERDSKGRRVIIQSPGKWNPSTTPMMDNIRAMYMTMELLIQSEETQVNGITILA--DHKGVRLAHVTNFTPSLMKKITTVMQDAFPIRIKGHNTVNEPSIFKAMFALMKPFLKEKMRKR 248
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003570.1 (pep scaffold:Pmarinus_7.0:GL486699:2092:8510:1 gene:ENSPMAG00000003262.1 transcript:ENSPMAT00000003570.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.855 bits (159), Expect = 1.537e-12
Identity = 42/147 (28.57%), Postives = 74/147 (50.34%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMD 767
            EL E  E     +++          +  P +  FLLRFLR  K+D+  A   +DNY   RR  P+    F  S++ +     V++  ++  L   D  G+ +I+++ GLW+P      D++RVL+M ++++  +  TQ  G+ V+ D
Sbjct:   43 ELQEKPEWRLRDIQALRDMLKEHPEIPAPTDDGFLLRFLRARKFDYDKALLLIDNYYKCRRVWPDVFVNFYPSSVRH-----VLEAGVVTALPQPDAQGRRIIVLRPGLWNPAVAPATDMLRVLYMTLEMLVRQEETQVCGLVVLAD 184
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007468.1 (pep scaffold:Pmarinus_7.0:GL477833:5549:9144:1 gene:ENSPMAG00000006747.1 transcript:ENSPMAT00000007468.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.6102 bits (135), Expect = 4.934e-10
Identity = 35/132 (26.52%), Postives = 67/132 (50.76%), Query Frame = 3 
Query:  567 IDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWN-QCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKR 956
            + E++D   +  L   D  G+ VI+   G W+P++  +ID  + ++M ++L+     TQ +GIT++  +D     +  + N    L K    + ++ +P+R+K      EP IF    ++ +   K KM+KR
Sbjct:   19 VKELLDSGFIRVLPERDSKGRRVIIQSPGKWNPSTTPIIDNFKAMYMTLELLIQSEETQVNGITIL--VDHKGMGLAHVTNFTPSLMKKITTVIQDAFPIRIKGHHTINEPSIFKAMFALIKPFFKEKMQKR 148
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Match: EDO33762 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SR95])

HSP 1 Score: 49.6766 bits (117), Expect = 6.784e-7
Identity = 47/215 (21.86%), Postives = 90/215 (41.86%), Query Frame = 3 
Query:  420 NKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEV-IDLNLLVPLGCSDDGQLVILVQF-GLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            + E LLR+LR   ++   A   L ++    +++PE  K  +   L   + D     L  + P+G +      I+V F G+WD ++     L++ L + +  ++     Q+ G+ +++D     T     +   +  K    I+E  YP+  K++    +P       S     +  K+K R     N++  L   IP     LP ++ G    +D
Sbjct:   23 DDEMLLRYLRARGHNVVAAFALLCSHIEFHKNNPELLKGLNAFELRYILEDGFPCVLPYMDPVGST------IMVLFPGMWDTDTFSPDTLLQALMLTLMHLSESLTVQSRGVVLLVDFSEW-TSTQAAYLNIKYIKKIVTIFENCYPVVFKSVHLINQPWYISALISFLRPFLTGKVKDRVFLHGNNLTTLHDHIPP--ESLPSQFGGDRCEVD 228
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: ttpal (alpha tocopherol transfer protein like [Source:NCBI gene;Acc:101167039])

HSP 1 Score: 96.2857 bits (238), Expect = 1.238e-21
Identity = 66/222 (29.73%), Postives = 112/222 (50.45%), Query Frame = 3 
Query:  399 LHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSD-DGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCE-LFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            LH ++  +  FLLRFLR  K+D+  A + L NY   RR+ PE      +  L    +  V+DL  L  L   D  G+ ++ ++ G W PN    +D +R +++ ++ +     TQ +GI +++D       ++Q  N    L K    I ++ +P+R+K +    EP+IF    +I +  +K KM +R +   +D+  L   +P    +LP EY G +G LD
Sbjct:   73 LHTRL--DDAFLLRFLRARKFDYDRALQLLLNYHAGRRAWPEV-----FQDLKPSTVKHVLDLGFLTVLPRPDPSGRYILCLRPGKWKPNDYPFVDNVRAIYLTLEKLIQPEETQVNGIIILVDY--TGVGMSQASNPGPFLAKKVVSILQDGFPIRIKAVNIINEPLIFKGIFAIIKPFLKEKMAERYILHGSDLRSLHRNLPA--SVLPEEYGGTAGRLD 283
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: rlbp1b (retinaldehyde binding protein 1 [Source:NCBI gene;Acc:101165475])

HSP 1 Score: 64.6994 bits (156), Expect = 3.733e-11
Identity = 51/215 (23.72%), Postives = 94/215 (43.72%), Query Frame = 3 
Query:  432 LLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTL----RLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDA 1061
            ++RF+R  KYD + A + +  Y   RR +PE      +  L+ E +   I+      L   D  G++V+L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K + F  +P  F    ++ + LMK K+ +R       L+N  ++ D  I      LP E+NG+    D 
Sbjct:   96 MVRFIRARKYDVARAFELMKGYVRFRRDYPEL-----FENLTPEAVRSTIEAGYPGILHSRDKYGRVVLLFNIENWDYEEITFDEILRAYCVILEKLLENEETQINGFCIIENFKGFTMQQASGIKPTEL-KKMVDMLQDSFPARFKAVHFIHQPWYFTTTYNVVKPLMKGKLLERVFVHGDELENYYKEFDPEI------LPSEFNGKGSKYDG 298
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: CLVS1 (clavesin-1 [Source:NCBI gene;Acc:101158238])

HSP 1 Score: 56.6102 bits (135), Expect = 1.904e-8
Identity = 56/260 (21.54%), Postives = 111/260 (42.69%), Query Frame = 3 
Query:  294 SIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLK-VPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKI----WEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            S+ SE     ++ELNE+ + +   ++       +   +  +  + +F+LRFLR  K+D     + L  Y   R+ + +  + F    L   I   ++D    V       G+ ++++    WD +    ID++R + + ++++      Q +G  +I+D    + K        +L  N  K+     ++++P R   + F  +P       +I +  +K K +KR     N++  L   I  L   LP E+ G   P D
Sbjct:    7 SLSSEMMEKARLELNENPDTLHQDIQQVRDMIVTRPDIGFLRTDDDFILRFLRARKFDQLETFRLLAQYFQFRQQNLDMFQSFKVDDLG--IKRALMDGFPGVLESPDQHGRKILILFASNWDQSRNSFIDILRAILLSLEVLIENPELQINGFILIIDWSNFSFK-----QASKLTPNILKLAIEGLQDSFPARFGGIHFVNQPWYIHAMYTIIKPFLKDKTRKRIFLHGNNLNSLHQLI--LPECLPSEFGGTLPPYD 257
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: TTPA (alpha tocopherol transfer protein [Source:NCBI gene;Acc:101155881])

HSP 1 Score: 56.225 bits (134), Expect = 2.096e-8
Identity = 63/257 (24.51%), Postives = 109/257 (42.41%), Query Frame = 3 
Query:  297 IKSETENNWQVELNEHSENIDAHVESFAKWC--SSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            ++   EN +  EL + SE + A V    +    +       P +  FLL+FLR   +D  ++ K L NY   RR  PE S     S LS   +  ++ ++    L   D  G  V++ + G W+        + RV  M  ++I+ +T TQ  G+  I DL   +   + +     L K    +  +++P++++ +    EP  F    ++    +  K+K+R      D    D R      +LP EY G    +D
Sbjct:    6 VRKLKENFYLSELPDESEQLRAFVSRLRQAADEAGGGPAGPPLSDGFLLKFLRARDFDVELSLKLLLNYLRWRRDSPEIS-----SCLSPSSVLGLLHMSYHAVLPQRDHAGSRVLIYRIGQWNTKDWSPYQVFRVSLMTSEIISMETETQRRGLKAIFDLQGWSLG-HALQINPSLAKKISCVLSDSFPLKVRGIHLVNEPRFFRPVFAMIRPFLPEKIKQRIHMHGADFHS-DLRHFFHPHVLPPEYGGHGPGID 255
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000002803.2 (retinaldehyde-binding protein 1-like [Source:NCBI gene;Acc:101170080])

HSP 1 Score: 55.4546 bits (132), Expect = 3.332e-8
Identity = 48/214 (22.43%), Postives = 95/214 (44.39%), Query Frame = 3 
Query:  432 LLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDD-GQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTL----RLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            LLRFLR  K++   A + L  Y   R+ +PE      +  L+ E +   I+    V L   D  G++V+L     WD   +   +++R   + ++ +     TQ +G  +I +      +        EL K    + ++++P R K +    +P  F    ++ +  MK+K+ +R       L++ +++ D+ I      LP ++ G++   D
Sbjct:   87 LLRFLRARKFNVVRAHELLKGYVRFRKDYPEL-----FENLTPEAVRSTIEAGYPVVLPSRDKYGRVVLLFNIENWDLEEITFDEVLRAYCVILEKLLENEETQINGFVLIENFKGFTMQHASGIKTTEL-KKMVDMLQDSFPARFKAVHVIHQPWYFTTTYNVVKPFMKAKLLERVYVHGEELESYLKEFDANI------LPSDFEGKAPVAD 288
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009863.1 (SMESG000009863.1)

HSP 1 Score: 530.406 bits (1365), Expect = 0.000e+0
Identity = 253/260 (97.31%), Postives = 259/260 (99.62%), Query Frame = 3 
Query:  291 MSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLDALRS 1070
            MSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLK+PENKEFL+RFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEV+DLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREP+IFDIHCSISELLMKSKMKKRTLRLKNDIEKL +RIPGLMRLLPFEYNGQSGPLDALR+
Sbjct:    1 MSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKIPENKEFLMRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVLDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPVIFDIHCSISELLMKSKMKKRTLRLKNDIEKLATRIPGLMRLLPFEYNGQSGPLDALRN 260

HSP 2 Score: 113.235 bits (282), Expect = 5.695e-28
Identity = 54/55 (98.18%), Postives = 55/55 (100.00%), Query Frame = 1 
Query: 1114 DKWKREFKGTYASEHFILSQIEVIENKRFISKAKVNAANLEPVHAEGTFTKFDKF 1278
            +KWKREFKGTYASEHFILSQIEVIENKRFISKAKVNAANLEPVHAEGTFTKFDKF
Sbjct:  260 NKWKREFKGTYASEHFILSQIEVIENKRFISKAKVNAANLEPVHAEGTFTKFDKF 314
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009872.1 (SMESG000009872.1)

HSP 1 Score: 224.557 bits (571), Expect = 6.599e-70
Identity = 101/253 (39.92%), Postives = 164/253 (64.82%), Query Frame = 3 
Query:  303 SETENNWQVE-LNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            SE      VE LNE+ E ++AH+ES  +W  S  H+  P +  FLL+FLR AK+DH  A++RLDN+CT R S     +FF +  + ++++   +   + +PLG +D G+++ L++  +WDP  + ++ L+ ++FMG+D+I     +Q HGI +++DL+  + K  + W + +L K W K+ +E+YP+R+K  IF+ EP++FD+   +  L M  KMK+RT R+KNDI+K   +IPGL ++LP EYNGQ G L+
Sbjct:   11 SENSKKAAVEYLNENPEQVNAHIESLRRWVKSMPHINCPTDGNFLLKFLRVAKFDHDKAERRLDNFCTARSSKKGSEEFFKFFKIEDQVVQTFLSSKMHIPLGQNDAGEIIFLIRSNVWDPEKIPMVKLIVLIFMGLDIIINMEESQIHGIRMLIDLNDISRKSLESWAEPKLLKQWHKVVQESYPLRVKGQIFYNEPVLFDLIYELISLFMNDKMKERTFRVKNDIKKAMEKIPGLEKILPTEYNGQGGSLN 263
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009876.1 (SMESG000009876.1)

HSP 1 Score: 211.846 bits (538), Expect = 6.444e-65
Identity = 93/240 (38.75%), Postives = 153/240 (63.75%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSG 1049
            ++ E+   ++AH+ESF +W  S  HL+ P + +FL++FLR +K+DH  A+KR+DNYCT+R S      FF+  ++++ II+  ++  + +PLG +++G+ + LV+  +WDP  +    L+ ++FM +D+I     TQ HG+ +I+DL  A+ K  + W   +  K   ++ +E+YP+R+K  IF+ EP+IFDI   +    M  KMK RT+R+KNDI K   + PGL ++LP EY GQ G
Sbjct:   21 DIGENPSQVNAHIESFRRWLKSMPHLQCPTDAKFLMKFLRVSKFDHKRAEKRIDNYCTVRASPRGSPSFFNTFSINDPIINTYLETGMHLPLGRTENGETIFLVRTKVWDPEQIPFPKLIILMFMAMDIICNDEETQIHGMKMIIDLKDASRKAMESWGDPKYMKQINRVMQESYPLRIKGTIFYNEPVIFDIVFQVLSFFMNEKMKSRTIRIKNDINKAIEKNPGLDKILPMEYGGQGG 260
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009874.1 (SMESG000009874.1)

HSP 1 Score: 200.675 bits (509), Expect = 1.136e-60
Identity = 91/243 (37.45%), Postives = 151/243 (62.14%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPLD 1058
            +L E+   ++AH+ESF +W  S  HL  P + EFLL+FLR +K+DH   QKRLDN+CT+R S      FF +  + + I+D  +D  + +PLG +D G++++L++   WD   + +   + + FMG+D++ ++  TQ HG+T+++DL   + K  +   + ++ K +    +++YP+R+K  IF  EP+IFD+   I    M  KM+ RT R+KNDI K   ++PG+  +LP EYNG+ G L+
Sbjct:   21 DLGENPSQVNAHIESFRRWVKSMPHLICPTDSEFLLKFLRVSKFDHKKTQKRLDNFCTVRSSPRGSPDFFHYFNVDDPIVDMYLDSKVQLPLGRNDAGEMILLMKPEGWDSEKIPVNQFLSLFFMGLDILLSEEETQIHGLTLLIDLKDVSRKSIEAMAEPKILKQYNMAVQDSYPIRMKKQIFCNEPVIFDVVFQIYSFFMNDKMRGRTHRIKNDINKAFEKVPGIKTVLPTEYNGEGGTLE 263
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000009877.1 (SMESG000009877.1)

HSP 1 Score: 199.519 bits (506), Expect = 3.661e-60
Identity = 96/242 (39.67%), Postives = 151/242 (62.40%), Query Frame = 3 
Query:  330 ELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLRFLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLNLLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQTHGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFREPIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNGQSGPL 1055
            E+ E    ++AH+ESF +W  S  HLK P + +FLL FLRTAK+DHSVAQ+RLDN+CT+R S     +FF + +L N II++V+  +  +P+G    G++V+L + G W P  + + ++++V++M  DLI  +  TQ +GI  I DL+    K  + W+   L KN  +IW++ +P+R+K +I ++ P +F I   I E  M  K KKR L++ +  +KL S++ GL  ++P EY G  G L
Sbjct:   21 EIKEDKNQVNAHIESFRRWVVSMPHLKCPTDSKFLLMFLRTAKFDHSVAQRRLDNFCTIRSSPRGSPRFFKFPSLKNSIIEKVLRSHQNIPMGKDSAGEMVLLFKTGDWKPEEISVENILQVVYMTFDLILTRESTQINGIVSIFDLEGCCRKTLEAWSDPHLTKNIMRIWQDAFPIRMKGIILYKPPTLFHIILKIMEFFMIDKHKKRILQIDSQ-DKLFSKVSGLKEIMPTEYGGNGGKL 261
The following BLAST results are available for this feature:
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TTPAL4.844e-1527.01alpha tocopherol transfer protein like [Source:HGN... [more]
TTPAL4.844e-1527.01alpha tocopherol transfer protein like [Source:HGN... [more]
TTPA1.808e-1226.76alpha tocopherol transfer protein [Source:HGNC Sym... [more]
CLVS26.471e-821.37clavesin 2 [Source:HGNC Symbol;Acc:HGNC:23046][more]
RLBP13.254e-719.43retinaldehyde binding protein 1 [Source:HGNC Symbo... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG339653.056e-1827.94gene:FBgn0053965 transcript:FBtr0100006[more]
CG129268.605e-1725.48gene:FBgn0033437 transcript:FBtr0088502[more]
CG316364.400e-1625.20gene:FBgn0051636 transcript:FBtr0079324[more]
CG316364.400e-1625.20gene:FBgn0051636 transcript:FBtr0343865[more]
CG335145.971e-1625.10gene:FBgn0053514 transcript:FBtr0091456[more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal2.468e-2230.70tocopherol (alpha) transfer protein-like [Source:Z... [more]
ttpal6.828e-2231.13tocopherol (alpha) transfer protein-like [Source:Z... [more]
rlbp1b2.130e-1123.22retinaldehyde binding protein 1b [Source:ZFIN;Acc:... [more]
rlbp1b2.130e-1123.22retinaldehyde binding protein 1b [Source:ZFIN;Acc:... [more]
rlbp1b2.130e-1123.22retinaldehyde binding protein 1b [Source:ZFIN;Acc:... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal1.196e-1927.73tocopherol (alpha) transfer protein-like [Source:N... [more]
rlbp13.754e-1121.05retinaldehyde binding protein 1 [Source:NCBI gene;... [more]
rlbp14.727e-1121.05retinaldehyde binding protein 1 [Source:NCBI gene;... [more]
ggh1.143e-1026.36gamma-glutamyl hydrolase (conjugase, folylpolygamm... [more]
thg1l1.618e-721.37tRNA-histidine guanylyltransferase 1-like [Source:... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ttpal1.432e-1526.82tocopherol (alpha) transfer protein-like [Source:M... [more]
Ttpal1.432e-1526.82tocopherol (alpha) transfer protein-like [Source:M... [more]
Ttpa1.608e-1123.55tocopherol (alpha) transfer protein [Source:MGI Sy... [more]
Ttpa1.907e-1023.47tocopherol (alpha) transfer protein [Source:MGI Sy... [more]
Clvs22.367e-821.43clavesin 2 [Source:MGI Symbol;Acc:MGI:2443223][more]
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BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9D3D0|TTPAL_MOUSE1.002e-1426.82Alpha-tocopherol transfer protein-like OS=Mus musc... [more]
sp|Q9BTX7|TTPAL_HUMAN2.326e-1427.01Alpha-tocopherol transfer protein-like OS=Homo sap... [more]
sp|Q5RFR0|TTPAL_PONAB2.459e-1427.01Alpha-tocopherol transfer protein-like OS=Pongo ab... [more]
sp|P49638|TTPA_HUMAN8.683e-1226.76Alpha-tocopherol transfer protein OS=Homo sapiens ... [more]
sp|P41034|TTPA_RAT5.512e-1123.70Alpha-tocopherol transfer protein OS=Rattus norveg... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4P3R67.957e-5033.88CRAL-TRIO domain-containing protein OS=Paragonimus... [more]
A0A4S2LBZ05.231e-4934.40CRAL-TRIO domain-containing protein OS=Opisthorchi... [more]
A0A1S8X0L33.177e-4834.14CRAL-TRIO domain-containing protein OS=Opisthorchi... [more]
A0A4S2LBM13.765e-4833.99CRAL-TRIO domain-containing protein OS=Opisthorchi... [more]
A0A267E9H49.668e-4837.10CRAL-TRIO domain-containing protein OS=Macrostomum... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal3.970e-2130.66alpha tocopherol transfer protein like [Source:NCB... [more]
ttpal7.634e-2130.66alpha tocopherol transfer protein like [Source:NCB... [more]
rlbp1b1.725e-1123.83retinaldehyde-binding protein 1 [Source:NCBI gene;... [more]
ttpa6.723e-924.88tocopherol (alpha) transfer protein [Source:ZFIN;A... [more]
rlbp1a1.565e-821.88retinaldehyde-binding protein 1-like [Source:NCBI ... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ttpal5.061e-1825.11tocopherol (alpha) transfer protein-like [Source:Z... [more]
ENSPMAT00000003570.11.537e-1228.57pep scaffold:Pmarinus_7.0:GL486699:2092:8510:1 gen... [more]
ENSPMAT00000007468.14.934e-1026.52pep scaffold:Pmarinus_7.0:GL477833:5549:9144:1 gen... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO337626.784e-721.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ttpal1.238e-2129.73alpha tocopherol transfer protein like [Source:NCB... [more]
rlbp1b3.733e-1123.72retinaldehyde binding protein 1 [Source:NCBI gene;... [more]
CLVS11.904e-821.54clavesin-1 [Source:NCBI gene;Acc:101158238][more]
TTPA2.096e-824.51alpha tocopherol transfer protein [Source:NCBI gen... [more]
ENSORLT00000002803.23.332e-822.43retinaldehyde-binding protein 1-like [Source:NCBI ... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009863.15.695e-2897.31SMESG000009863.1[more]
SMESG000009872.16.599e-7039.92SMESG000009872.1[more]
SMESG000009876.16.444e-6538.75SMESG000009876.1[more]
SMESG000009874.11.136e-6037.45SMESG000009874.1[more]
SMESG000009877.13.661e-6039.67SMESG000009877.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008074 ID=SMED30008074|Name=CRAL-TRIO domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1310bp
GTGAATAAAAGTTTTATTAATTTAATCACAGTCAGCATTGGTGGTTTGAA
AGTAGAGTGATCTTACAATCGAATCAATTGTGTTTGGAAAGCAACATAAT
GGGAGCCTTTGGCGGCTGTGATATGATAATTATATACATATCACAGTCAT
GCTAAAGAGCTTGTAAATTGTTACTAATCGTCTCGTGCTTCACTGATCAG
TTTGTCAATTTTAATAATTGATGCGAATTTTGTGTGCAAAGTTTTATATA
TATAATAAGATCGAGTTGAAAAAAGTAAACAAATTGAAAAATGTCAATAA
AATCTGAGACTGAAAATAACTGGCAAGTTGAACTCAATGAGCATTCTGAA
AATATTGATGCTCACGTTGAAAGCTTTGCTAAGTGGTGTAGTAGTACCTT
ACATCTAAAAGTTCCTGAAAATAAAGAATTTTTGTTGAGGTTTTTGCGAA
CTGCTAAATACGATCATTCAGTTGCTCAAAAAAGACTAGATAATTACTGC
ACGTTACGTCGTTCCCATCCAGAATGTTCTAAATTTTTCGATTGGTCAAC
TTTAAGCAATGAGATTATTGATGAAGTTATAGATTTGAATTTGTTAGTTC
CTTTGGGTTGCAGTGATGATGGGCAATTAGTGATATTGGTACAGTTTGGA
TTGTGGGACCCAAATAGTGTCAAATTAATTGATCTAATGAGGGTTCTATT
TATGGGCATAGACTTAATTGCTGCTAAAACGATAACTCAAACTCATGGAA
TAACAGTAATTATGGATTTGGATCGAGCAAATACAAAGATAAATCAAATT
TGGAATCAGTGCGAGTTATTTAAAAATTGGAAAAAAATTTGGGAAGAAAC
TTATCCTATGAGACTGAAAAACTTGATTTTCTTCAGAGAACCGATTATTT
TTGATATACATTGTTCGATTTCTGAACTACTTATGAAATCCAAAATGAAG
AAAAGGACTTTGCGGTTGAAAAACGATATTGAAAAATTAGATTCTAGAAT
TCCAGGATTGATGCGATTACTGCCTTTTGAATATAATGGACAAAGTGGAC
CGCTTGATGCTCTCAGAAGTATACTTAATGATTTTAAGCTTGAAAATTTT
ATCAATTTAATTAGATAAATGGAAAAGAGAGTTCAAAGGAACTTATGCTT
CTGAACATTTTATTCTGAGCCAAATCGAAGTGATTGAAAATAAACGTTTC
ATTTCCAAGGCAAAAGTTAATGCGGCTAATTTAGAACCAGTTCATGCTGA
AGGCACTTTTACTAAATTTGATAAATTTTAAATATATAATTTTTTTTCTT
TGATATTTAA
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protein sequence of SMED30008074-orf-1

>SMED30008074-orf-1 ID=SMED30008074-orf-1|Name=SMED30008074-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=276bp
MSIKSETENNWQVELNEHSENIDAHVESFAKWCSSTLHLKVPENKEFLLR
FLRTAKYDHSVAQKRLDNYCTLRRSHPECSKFFDWSTLSNEIIDEVIDLN
LLVPLGCSDDGQLVILVQFGLWDPNSVKLIDLMRVLFMGIDLIAAKTITQ
THGITVIMDLDRANTKINQIWNQCELFKNWKKIWEETYPMRLKNLIFFRE
PIIFDIHCSISELLMKSKMKKRTLRLKNDIEKLDSRIPGLMRLLPFEYNG
QSGPLDALRSILNDFKLENFINLIR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000016pharynx
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036865CRAL-TRIO_dom_sf
IPR036273CRAL/TRIO_N_dom_sf
IPR001071CRAL-bd_toc_tran
IPR011074CRAL/TRIO_N_dom
IPR001251CRAL-TRIO_dom
Vocabulary: biological process
TermDefinition
GO:0006810transport
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0005215transporter activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00180CRETINALDHBPcoord: 175..196
score: 11.36
coord: 47..69
score: 40.22
coord: 242..251
score: 40.0
NoneNo IPR availableGENE3DG3DSA:1.10.8.20coord: 1..75
e-value: 4.3E-7
score: 31.9
NoneNo IPR availablePANTHERPTHR10174RETINALDEHYDE BINDING PROTEIN-RELATEDcoord: 5..263
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3DG3DSA:3.40.525.10coord: 83..259
e-value: 5.9E-27
score: 96.0
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILYSSF52087CRAL/TRIO domaincoord: 94..254
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 105..239
e-value: 2.8E-15
score: 56.4
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 89..256
score: 12.408
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 93..251
e-value: 3.13118E-14
score: 66.6398
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILYSSF46938CRAL/TRIO N-terminal domaincoord: 13..82