SMED30008062
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30008062 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30008062 aligns in the following genomic locations:
Homology
BLAST of SMED30008062 vs. TrEMBL
Match: A0A1S3IZ14 (uncharacterized protein LOC106168312 OS=Lingula unguis OX=7574 GN=LOC106168312 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 1.434e-12 Identity = 49/166 (29.52%), Postives = 97/166 (58.43%), Query Frame = 1 Query: 292 GGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKV--GEDTQISAGVAKEINR-------------KCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLL 744 G V++E +L + E T +YK+ + ++ L ++Y ++ RYR LKSMIKR V HL++ G D +S+G+ K++ R + EE+Y+ L+ + L+E+ ++ QI + +T I++++ +++ QY SK+Y+A+ RY+++ +IK+++ Sbjct: 15 GQSEVRRETEQLCNDLEATQQDYKKFRLLVEQLHREY-VESKELDFFRRYRQLKSMIKRTVTHLRLSNGGDA-LSSGL-KQMCRDFVRQKDTSAAAQRIEERYKDLSDDALLEQNVEIQEQITDTKRKTDIIKDLIHEMEVQYEESKRYIAIQRYRLMKGMIKEVI 177
BLAST of SMED30008062 vs. TrEMBL
Match: R7V1A0 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_200340 PE=4 SV=1) HSP 1 Score: 73.9442 bits (180), Expect = 3.200e-12 Identity = 41/135 (30.37%), Postives = 81/135 (60.00%), Query Frame = 1 Query: 355 EYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQ-----ISAGVAKEINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLL 744 EYK L+ ++ L+++Y + + + RY MLK MIKR V+HLK+ + + + G KE NRK EE L+ + + +E ++ QI+ ++ + ++++++ +D Y SK+Y+A+ RY+++ +IK ++ Sbjct: 35 EYKHLRGVVEQLSREYEDSKEVD-FFRRYGMLKGMIKRSVLHLKLTSNEEHNPHVPTCGKQKEENRKREEHIVGLSKDEITQENDEIQEQIKEYRRKISMMKDLIEQMDHSYEVSKRYIAVQRYRLMKVMIKTVV 168
BLAST of SMED30008062 vs. TrEMBL
Match: V4A728 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_229008 PE=4 SV=1) HSP 1 Score: 69.707 bits (169), Expect = 3.376e-10 Identity = 46/165 (27.88%), Postives = 88/165 (53.33%), Query Frame = 1 Query: 298 ENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAKEI----------------NRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLL 744 + +++E +L + E YK+L+Q +D L ++Y + + RY LK+M+KR ++H K+ D Q SA ++ I ++ EE+ +L+ E +I + + +QI+ + +I+R + +L+ Y +SK+YV L RY+ML +IK ++ Sbjct: 61 DEIRREHEQLSNDLESVQRNYKKLRQVVDQLEREYE-ESKEFDPVRRYEKLKNMVKRTILHFKLDPDQQGSASQSQGIVGLMQGCKQYAQHLESTKRREERCSRLSREDIIIANSKMGDQIKECNRKCSIIRGLVENLERGYESSKRYVVLQRYRMLKTMIKTVI 224
BLAST of SMED30008062 vs. TrEMBL
Match: A0A210QLH3 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT06340 PE=4 SV=1) HSP 1 Score: 62.003 bits (149), Expect = 1.066e-7 Identity = 37/144 (25.69%), Postives = 82/144 (56.94%), Query Frame = 1 Query: 355 EYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISA-GVAKEIN-------------RKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLL 744 E+K+LKQ ++ LA+DY + + L RY +K MIKR ++HL++ +D + S+ G++ I R+ + + ++ + + +E L+ Q++ + +++R++ + LD Y +SK + + RY+++ +IK+++ Sbjct: 55 EHKKLKQVVEQLARDYEESKYFDP-LRRYEKMKGMIKRTILHLRLNQDDKGSSCGISGLIQSCRDYAHQVEAGKRREQHQSANMSKQEIAQENESLRTQVKDMRRECSVIRDIINQLDQNYESSKHFSLIQRYRVMKLMIKNVI 197
BLAST of SMED30008062 vs. TrEMBL
Match: K1RK99 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10028224 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 4.031e-6 Identity = 39/148 (26.35%), Postives = 76/148 (51.35%), Query Frame = 1 Query: 355 EYKRLKQHIDTLAKDYSCLQTSNNW--LSRYRMLKSMIKRCVMHLKVGEDTQISAGVAKEINRKCEEKYEKLN----------------SETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLL 744 EYK ++Q +DTL +DY + S ++ L RY +LK MIKR ++H K+ ED ++ V+ R Y++L+ E + E L++QI + R +++ + + L+++Y SK + RY ++ +++ ++ Sbjct: 52 EYKHVRQVVDTLQQDY---ERSKDFDPLRRYGLLKGMIKRTILHFKLNED-KLPGSVSMTGIRSLANSYKELSYTMELTRRKADLDDMCVEEIESEVEQLRHQINGLRGRCSLVTDTVTQLESKYELSKNFAPPKRYDLMKKMVQKVV 195
BLAST of SMED30008062 vs. Planmine SMEST
Match: SMESG000023281.1 (SMESG000023281.1) HSP 1 Score: 449.899 bits (1156), Expect = 7.967e-162 Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 1 Query: 67 MLDIPNSSRNDLNSLDPKRYSENLGRSQSVRRTSNISNADGGXXXXXXXXXXXXXXXNENGGLRRMSKFLHESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAKEINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLLARPNVKELIS 774 MLDIPNSSRNDLNSLDPKRYSENLGRSQSVRRTSNISNADGGLLRSSSLRLPSSSKSNENGGLRRMSKFLHESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAKEINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLLARPNVKELIS Sbjct: 1 MLDIPNSSRNDLNSLDPKRYSENLGRSQSVRRTSNISNADGGLLRSSSLRLPSSSKSNENGGLRRMSKFLHESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAKEINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLLARPNVKELIS 236
BLAST of SMED30008062 vs. Planmine SMEST
Match: SMESG000069423.1 (SMESG000069423.1) HSP 1 Score: 118.627 bits (296), Expect = 9.242e-32 Identity = 71/180 (39.44%), Postives = 103/180 (57.22%), Query Frame = 1 Query: 253 LRRMSKFLH-----ESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAK--EINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLLARPNVKELI 771 LR+MS+F H E+LL K+ FI++Y+ + EYKRLK +++ L DY + ++RY LK MIKR +MHLKV + K E+ RK EE+YE L S+ + +EK +K IE Q R IL +DL+ Y +K Y TRYQM+ A++KDL+ +P + E+I Sbjct: 67 LRKMSQFFHVDKISETLLKDPE-KEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYE-MSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 244
BLAST of SMED30008062 vs. Planmine SMEST
Match: SMESG000044339.1 (SMESG000044339.1) HSP 1 Score: 74.7146 bits (182), Expect = 7.878e-16 Identity = 55/171 (32.16%), Postives = 89/171 (52.05%), Query Frame = 1 Query: 289 LGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQ-----------ISA------GVAKEINR-----KCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIK 735 L + V++++ +L + + + EYKRL+ +D L DYS S + + RY LK MIKR V+HLK+ T ISA G + R K EE+ + L SE +I E ++ I+ ++ +TA++ + ++L+ +YTASK Y + RY L +IK Sbjct: 24 LMKDPVREDYQRLAEEYDKSCEEYKRLRSFVDILEADYSS-SKSLDVIRRYLSLKGMIKRTVLHLKLPHITSGKSDLNPNAVGISAMGNLLQGTRTFVKRSEQAKKWEERLQTLKSEEIIAENRKMEESIKDYKRKTAMITDAINNLEVEYTASKIYFPIRRYPALKQIIK 193
BLAST of SMED30008062 vs. Planmine SMEST
Match: SMESG000032554.1 (SMESG000032554.1) HSP 1 Score: 54.299 bits (129), Expect = 1.176e-8 Identity = 40/148 (27.03%), Postives = 75/148 (50.68%), Query Frame = 1 Query: 355 EYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLK-----------------VGEDTQISAGVAK--EINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDL 741 + KR++ +I+TL ++Y+ + + + RY+ LK MIKR MHLK +G Q + + K E RK EE+ +K+ S+ LI E +++ ++ +++ A + + ++ Y S+ RYQ L +IK++ Sbjct: 40 DLKRIQGYIETLEEEYNQCKMMDVF-RRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNV 186
BLAST of SMED30008062 vs. Planmine SMEST
Match: SMESG000080507.1 (SMESG000080507.1) HSP 1 Score: 46.595 bits (109), Expect = 6.394e-6 Identity = 42/173 (24.28%), Postives = 84/173 (48.55%), Query Frame = 1 Query: 247 GGLRRMSKFLH-----ESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQ--ISAGVAKEINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLL 744 +R+MS+F + +S+ +N K+ F++L++ Q+ +E +R ID L Y + L+ Y K+MI+ V HLK+ T S KE+ K ++ Y+KL+++ +E K + I + + + + L+ Y K++ +Y+++ +IK + Sbjct: 36 NKIRKMSQFFNIEKFSDSIWNDDNEKEIFLQLFEQQDNLTLENRRFHSFIDDLKIKYEN-EKQRYSLNCYFNYKNMIQCAVNHLKISHVTGEICSNARLKEVVSKADKIYKKLSNQERLEYKNEMVIDINLLKQQISKFVYAIEQLEKDYENIKQFSFFRKYKLMKMIIKKCI 207 The following BLAST results are available for this feature:
BLAST of SMED30008062 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30008062 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30008062 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30008062 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30008062 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30008062 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008062 ID=SMED30008062|Name=SMED30008062|organism=Schmidtea mediterranea sexual|type=transcript|length=888bpback to top protein sequence of SMED30008062-orf-1 >SMED30008062-orf-1 ID=SMED30008062-orf-1|Name=SMED30008062-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=237bp MLDIPNSSRNDLNSLDPKRYSENLGRSQSVRRTSNISNADGGLLRSSSLRback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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