Malate dehydrogenase 1B

Overview
NameMalate dehydrogenase 1B
Smed IDSMED30008051
Length (bp)1950
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Malate dehydrogenase 1B (SMED30008051) t-SNE clustered cells

Violin plots show distribution of expression levels for Malate dehydrogenase 1B (SMED30008051) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Malate dehydrogenase 1B (SMED30008051) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Malate dehydrogenase 1B (SMED30008051) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008051

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30008051SMESG000070444.1 SmedASXL_015381SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
neuronSMED30008051SMESG000070444.1 dd_Smed_v4_10200_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30008051SMESG000070444.1 dd_Smed_v6_10200_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30008051SMESG000070444.1 dd_Smed_v4_10200_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ventral epidermisSMED30008051SMESG000070444.1 dd_Smed_v4_10200_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30008051SMESG000070444.1 dd_Smed_v4_10200_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Malate dehydrogenase 1B vs. Ensembl Human
Match: MDH1B (malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HGNC:17836])

HSP 1 Score: 267.314 bits (682), Expect = 3.655e-81
Identity = 167/465 (35.91%), Postives = 260/465 (55.91%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            MAK V+AG +DCPY+AK EL+AD L+ N+P+F +HKI +  + W  W  ++C+ N WS K+SPIIWREL+DRGGKG L+GG NEF E+ + YY+V S  T+  M  IA +N+     +++E    K    P +V I  A   A  + +  L+ G+V   +TE++I   D  +    LKSL + T DLA  ++RS+SI    E AF  + ++V+ DD         E    +++        G  IEK     V+V+   +   +++N+  + + R   RI   N++ ++  VE + ++++ARKL   PS + + I+WGNI G  Y+D   +RV+RY+ A+ GP  +S  V   ++D +W+K E   ++ N    ++      G  LA A +I T L  W +G   G I SL I SEG +G+P G+ FSMPV     G W V+ D++  EI  + +     D+I++
Sbjct:    1 MAKFVIAGRADCPYYAKTELVADYLQKNLPDFRIHKITQRPEVWEDWLKDVCEKNKWSHKNSPIIWRELLDRGGKGLLLGGYNEFLEHAQLYYDVTSSMTTELMMVIAQENLGAHIEKEQEEEALKTCINPLQVWITSASAPACYNLIPILTSGEVFGMHTEISITLFDNKQAEEHLKSLVVETQDLASPVLRSVSICTKVEEAFRQAHVIVVLDDSTNKEVFTLE--DCLRSRVPLCRLYGYLIEKNAHESVRVI---VGGRTFVNLKTVLLMRYAPRIAH-NIIAVALGVEGEAKAILARKLKTAPSYIKDVIIWGNISGNNYVDLRKTRVYRYESAIWGPLHYSRPVLNLIFDSEWVKREFVAILKN----LTTTGRQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGILSEGQFGIPKGIVFSMPVKFE-NGTWVVLTDLKDVEISEQIMTRMTSDLIQE 453          
BLAST of Malate dehydrogenase 1B vs. Ensembl Human
Match: MDH1B (malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HGNC:17836])

HSP 1 Score: 266.929 bits (681), Expect = 4.879e-81
Identity = 167/465 (35.91%), Postives = 260/465 (55.91%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            MAK V+AG +DCPY+AK EL+AD L+ N+P+F +HKI +  + W  W  ++C+ N WS K+SPIIWREL+DRGGKG L+GG NEF E+ + YY+V S  T+  M  IA +N+     +++E    K    P +V I  A   A  + +  L+ G+V   +TE++I   D  +    LKSL + T DLA  ++RS+SI    E AF  + ++V+ DD         E    +++        G  IEK     V+V+   +   +++N+  + + R   RI   N++ ++  VE + ++++ARKL   PS + + I+WGNI G  Y+D   +RV+RY+ A+ GP  +S  V   ++D +W+K E   ++ N    ++      G  LA A +I T L  W +G   G I SL I SEG +G+P G+ FSMPV     G W V+ D++  EI  + +     D+I++
Sbjct:    1 MAKFVIAGRADCPYYAKTELVADYLQKNLPDFRIHKITQRPEVWEDWLKDVCEKNKWSHKNSPIIWRELLDRGGKGLLLGGYNEFLEHAQLYYDVTSSMTTELMMVIAQENLGAHIEKEQEEEALKTCINPLQVWITSASAPACYNLIPILTSGEVFGMHTEISITLFDNKQAEEHLKSLVVETQDLASPVLRSVSICTKVEEAFRQAHVIVVLDDSTNKEVFTLE--DCLRSRVPLCRLYGYLIEKNAHESVRVI---VGGRTFVNLKTVLLMRYAPRIAH-NIIAVALGVEGEAKAILARKLKTAPSYIKDVIIWGNISGNNYVDLRKTRVYRYESAIWGPLHYSRPVLNLIFDSEWVKREFVAILKN----LTTTGRQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGILSEGQFGIPKGIVFSMPVKFE-NGTWVVLTDLKDVEISEQIMTRMTSDLIQE 453          
BLAST of Malate dehydrogenase 1B vs. Ensembl Human
Match: MDH1B (malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HGNC:17836])

HSP 1 Score: 171.4 bits (433), Expect = 1.915e-46
Identity = 112/367 (30.52%), Postives = 186/367 (50.68%), Query Frame = 2
Query:  332 FTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
             T+  M  IA +N+     +++E    K    P +V I  A   A  + +  L+ G+V   +TE++I   D  +    LKSL + T DLA  ++RS+SI    E AF  + ++V+ DD         E    +++        G  IEK     V+V+   +   +++N+  + + R   RI   N++ ++  VE + ++++ARKL   PS + + I+WGNI G  Y+D   +RV+RY+ A+ GP  +S  V   ++D +W+K E   ++ N    ++      G  LA A +I T L  W +G   G I SL I SEG +G+P G+ FSMPV     G W V+ D++  EI  + +     D+I++
Sbjct:    1 MTTELMMVIAQENLGAHIEKEQEEEALKTCINPLQVWITSASAPACYNLIPILTSGEVFGMHTEISITLFDNKQAEEHLKSLVVETQDLASPVLRSVSICTKVEEAFRQAHVIVVLDDSTNKEVFTLE--DCLRSRVPLCRLYGYLIEKNAHESVRVI---VGGRTFVNLKTVLLMRYAPRIAH-NIIAVALGVEGEAKAILARKLKTAPSYIKDVIIWGNISGNNYVDLRKTRVYRYESAIWGPLHYSRPVLNLIFDSEWVKREFVAILKN----LTTTGRQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGILSEGQFGIPKGIVFSMPVKFE-NGTWVVLTDLKDVEISEQIMTRMTSDLIQE 355          
BLAST of Malate dehydrogenase 1B vs. Ensembl Human
Match: MDH1B (malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HGNC:17836])

HSP 1 Score: 108.612 bits (270), Expect = 2.832e-27
Identity = 63/133 (47.37%), Postives = 89/133 (66.92%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTK 436
            MAK V+AG +DCPY+AK EL+AD L+ N+P+F +HKI +  + W  W  ++C+ N WS K+SPIIWREL+DRGGKG L+GG NEF E+ + YY+V S  T+  M  IA +N+     +++E    K    P +
Sbjct:    1 MAKFVIAGRADCPYYAKTELVADYLQKNLPDFRIHKITQRPEVWEDWLKDVCEKNKWSHKNSPIIWRELLDRGGKGLLLGGYNEFLEHAQLYYDVTSSMTTELMMVIAQENLGAHIEKEQEEEALKTCINPLQ 133          
BLAST of Malate dehydrogenase 1B vs. Ensembl Human
Match: MDH1B (malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HGNC:17836])

HSP 1 Score: 111.694 bits (278), Expect = 1.287e-26
Identity = 65/136 (47.79%), Postives = 91/136 (66.91%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVI 445
            MAK V+AG +DCPY+AK EL+AD L+ N+P+F +HKI +  + W  W  ++C+ N WS K+SPIIWREL+DRGGKG L+GG NEF E+ + YY+V S  T+  M  IA +N+     +++E    K    P +V I
Sbjct:    1 MAKFVIAGRADCPYYAKTELVADYLQKNLPDFRIHKITQRPEVWEDWLKDVCEKNKWSHKNSPIIWRELLDRGGKGLLLGGYNEFLEHAQLYYDVTSSMTTELMMVIAQENLGAHIEKEQEEEALKTCINPLQVWI 136          

HSP 2 Score: 57.7658 bits (138), Expect = 1.776e-8
Identity = 39/113 (34.51%), Postives = 58/113 (51.33%), Query Frame = 2
Query: 1097 VW-DKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            VW   +W+K E   ++ N    ++      G  LA A +I T L  W +G   G I SL I SEG +G+P G+ FSMPV     G W V+ D++  EI  + +     D+I++
Sbjct:  134 VWITSEWVKREFVAILKN----LTTTGRQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGILSEGQFGIPKGIVFSMPVKFE-NGTWVVLTDLKDVEISEQIMTRMTSDLIQE 240          
BLAST of Malate dehydrogenase 1B vs. Ensembl Celegans
Match: mdh-1 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q8IA49])

HSP 1 Score: 76.2554 bits (186), Expect = 8.481e-15
Identity = 79/341 (23.17%), Postives = 145/341 (42.52%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFA--DSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNIN-LISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            +S P +V++ GA  +     V+ ++ G V      + +V +D  +   IL+ +     D A   + S+    + ++AF   D + +V    R       D     + AN + ++  G  + +Y     KV++V   A    N N  I+      +IP  N   ++R    +  + +A K      NV N I+WGN  G  + D  ++ V++      G E  + +    V D  +L+      +  R   I +    L + ++ A A    +  W  G + G   S+ + S+G YG+P G+ FS PVT+   G W +VQ +   +     I    +++ E+
Sbjct:    1 MSAPLRVLVTGAAGQIGYSIVIRIADGTVFGKEQPVELVLLDVPQCSNILEGVVFELQDCALPTLFSVVAVTDEKSAFTGIDYAFLVGAMPRREGMERKD----LLAANVKIFKSQGKALAEYAKPTTKVIVVGNPA----NTNAFIAAKYAAGKIPAKNFSAMTRLDHNRALAQLALKTGTTIGNVKNVIIWGNHSGTQFPDVTHATVNKN-----GTETDAYA---AVGDNAFLQGPFIATVQKRGGVIIEKRK-LSSAMSAAKAACDHIHDWHFGTKAGQFVSMAVPSDGSYGIPQGLIFSFPVTIE-GGEWKIVQGLSFDDFAKGKIAATTKELEEE 323          
BLAST of Malate dehydrogenase 1B vs. Ensembl Celegans
Match: mdh-1 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q8IA49])

HSP 1 Score: 51.9878 bits (123), Expect = 1.934e-7
Identity = 44/178 (24.72%), Postives = 79/178 (44.38%), Query Frame = 2
Query:  899 LSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++R    +  + +A K      NV N I+WGN  G  + D  ++ V++      G E  + +    V D  +L+      +  R   I +    L + ++ A A    +  W  G + G   S+ + S+G YG+P G+ FS PVT+   G W +VQ +   +     I    +++ E+
Sbjct:    1 MTRLDHNRALAQLALKTGTTIGNVKNVIIWGNHSGTQFPDVTHATVNKN-----GTETDAYA---AVGDNAFLQGPFIATVQKRGGVIIE-KRKLSSAMSAAKAACDHIHDWHFGTKAGQFVSMAVPSDGSYGIPQGLIFSFPVTIE-GGEWKIVQGLSFDDFAKGKIAATTKELEEE 168          
BLAST of Malate dehydrogenase 1B vs. Ensembl Celegans
Match: mdh-1 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q8IA49])

HSP 1 Score: 50.8322 bits (120), Expect = 1.264e-6
Identity = 48/211 (22.75%), Postives = 90/211 (42.65%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFA--DSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNIN-LISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHR 1042
            +S P +V++ GA  +     V+ ++ G V      + +V +D  +   IL+ +     D A   + S+    + ++AF   D + +V    R       D     + AN + ++  G  + +Y     KV++V   A    N N  I+      +IP  N   ++R    +  + +A K      NV N I+WGN  G  + D  ++ V++
Sbjct:    1 MSAPLRVLVTGAAGQIGYSIVIRIADGTVFGKEQPVELVLLDVPQCSNILEGVVFELQDCALPTLFSVVAVTDEKSAFTGIDYAFLVGAMPRREGMERKD----LLAANVKIFKSQGKALAEYAKPTTKVIVVGNPA----NTNAFIAAKYAAGKIPAKNFSAMTRLDHNRALAQLALKTGTTIGNVKNVIIWGNHSGTQFPDVTHATVNK 203          
BLAST of Malate dehydrogenase 1B vs. Ensembl Fly
Match: Mdh1 (gene:FBgn0262782 transcript:FBtr0345435)

HSP 1 Score: 78.9518 bits (193), Expect = 1.424e-15
Identity = 77/322 (23.91%), Postives = 136/322 (42.24%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSE 1384
            ++ P +VV+ GA  +     +  ++RG+V   +  + +  +D    + +L+ + M   D A  L+  +  T +    F D S   +         +  E    + AN + +   G  ++K+   DVKVL+V   A    N N +        IP+ N   ++R  + +  S +A KL V  S V N I+WGN     Y D   ++V   +G V        SV   + D  +L+      +  R   +      + + ++ A A    +  W NG   G   S+ + S+G Y  P  + FS PV +  +  W +V  + LS+
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLYMIARGEVFGKDQPIVLHLLDIPPMVGVLEGVVMELADCALPLLVEVVPTTDPAVGFKDVSAAFLVGAM--PRKEGMERKDLLSANVKIFRTQGQALDKFAKKDVKVLVVGNPA----NTNALVCSSYAPSIPRENFSAMTRLDQNRATSQIAAKLGVPISAVKNIIIWGNHSSTQYPDAGQAKV-TANGTVK-------SVVDAINDNGYLQGSFVETVQKRGAAVIAARK-MSSAMSAAKAACDHMHDWWNGTAPGQFVSMGVFSDGSYDSPKDVIFSFPVEIKNK-QWKIVSGLTLSD 306          
BLAST of Malate dehydrogenase 1B vs. Ensembl Fly
Match: Mdh1 (gene:FBgn0262782 transcript:FBtr0080050)

HSP 1 Score: 78.9518 bits (193), Expect = 1.424e-15
Identity = 77/322 (23.91%), Postives = 136/322 (42.24%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSE 1384
            ++ P +VV+ GA  +     +  ++RG+V   +  + +  +D    + +L+ + M   D A  L+  +  T +    F D S   +         +  E    + AN + +   G  ++K+   DVKVL+V   A    N N +        IP+ N   ++R  + +  S +A KL V  S V N I+WGN     Y D   ++V   +G V        SV   + D  +L+      +  R   +      + + ++ A A    +  W NG   G   S+ + S+G Y  P  + FS PV +  +  W +V  + LS+
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLYMIARGEVFGKDQPIVLHLLDIPPMVGVLEGVVMELADCALPLLVEVVPTTDPAVGFKDVSAAFLVGAM--PRKEGMERKDLLSANVKIFRTQGQALDKFAKKDVKVLVVGNPA----NTNALVCSSYAPSIPRENFSAMTRLDQNRATSQIAAKLGVPISAVKNIIIWGNHSSTQYPDAGQAKV-TANGTVK-------SVVDAINDNGYLQGSFVETVQKRGAAVIAARK-MSSAMSAAKAACDHMHDWWNGTAPGQFVSMGVFSDGSYDSPKDVIFSFPVEIKNK-QWKIVSGLTLSD 306          
BLAST of Malate dehydrogenase 1B vs. Ensembl Zebrafish
Match: mdh1b (malate dehydrogenase 1B, NAD (soluble) [Source:NCBI gene;Acc:767794])

HSP 1 Score: 251.136 bits (640), Expect = 5.512e-76
Identity = 152/463 (32.83%), Postives = 242/463 (52.27%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKE-NEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDI 1423
            MAK V+AG +DCPY+AK ELLAD L+ N+P+FH+HKI     +W  W  + C +  W    SP++WREL+DRGGKG L+GG ++F E+ + YYN+ SD  S  M  IA +N   KE   ++E+   K+L  P  + I  A+N      +  L    + +     ++  +D +    +L++L+M T DLA   +   +   N              D++N    D D+         E++   G  IE     DV+VL   +    ++N+    +      I   N V ++  +E + R+ +A+KL+V  S++ N IVWGNI G  +ID   ++V RYDGA+ GP+ FS  V + ++D KWLK +   L+      IS   N   T ++  +AI   L  W N +    + SL + S G +G+P G+ FSMPV+    G+W+V  DV +++    +++ C  ++
Sbjct:    8 MAKFVLAGKADCPYYAKAELLADVLQRNLPDFHIHKIPVPPDDWKKWLEDTCASIGWKHSSSPLVWRELIDRGGKGMLLGGFSDFLEHAQGYYNITSDMNSDLMLKIAEENQATKELCMEEEVHRLKSLR-PLHIWISSALNPVCYSLIPHLFTPGLFSGLPIFSLHLMDTSGSEEMLQALKMETVDLAIPRLHEPAGEYN--------------DEQN----DKDQV-------AEHFHRYGQLIETNAQKDVRVL---VAGDFFINMKCSLLIENAPSIDPRNFVAMTTQLEYEARTQLAQKLSVKTSDITNVIVWGNISGSFHIDLQRAKVFRYDGAIRGPDGFSQHVMEMIYDWKWLKTDFRSLLHKHRATISSKTNK-ATAISTTNAILAILKAWNNAESPEEVFSLGVLSTGQFGIPAGLVFSMPVSFT-DGHWSVRSDVIVTDELRVNLDACADEL 439          
BLAST of Malate dehydrogenase 1B vs. Ensembl Zebrafish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 111.694 bits (278), Expect = 2.271e-26
Identity = 92/345 (26.67%), Postives = 155/345 (44.93%), Query Frame = 2
Query:  401 IREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            +R   A + P +V++ GA  +     + S+++GDV   +  + +V +D T  LP+L  + M   D A  L+R +  TD  E  F D    ++    +    +  E    +KAN   ++  G  +EKY    VKVL+V   A    N N +   +    IPK N   L+R    + RS VA ++ V   +V N  +WGN     Y D +++ V R      G E   ++    V D+ WLK +    +  R   +      L + ++ A AI   +     G   G   S+ I S G  YGVPD + +S PV +  +  W VV  + +++     ++    +++E+
Sbjct:   12 VRTASAQAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDITPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVG--SMPRKEGMERKDLLKANVAIFKTQGEALEKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVPSDSVKNVTIWGNHSSTQYPDVHHAIVTRN-----GKE---IAAFDAVNDESWLKGDFISTVQQRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTPDGEWVSMGIYSSGNSYGVPDDLMYSFPVKIKNKS-WKVVDGLSINDFSRGKMDATAAELVEE 340          
BLAST of Malate dehydrogenase 1B vs. Ensembl Zebrafish
Match: mdh1ab (malate dehydrogenase 1Ab, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-030131-7655])

HSP 1 Score: 110.923 bits (276), Expect = 3.270e-26
Identity = 85/338 (25.15%), Postives = 153/338 (45.27%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            +S P +V++ GA  +     +  +++GDV   +  L ++ +D T  LP+L+ + M   D A  L+R +  TD  + AF D    ++         +  E    +KAN   ++  G  ++KY    VKVL+V   A    N N +   +    IPK N   L+R    +  S VA +  + P+NV N I+WGN     Y D ++ +V+     V G +  +    K   D  WLK E    +  R   +      L + ++ A AI   +  W    E  +I   V ++   YGVP+ + +S P+++  +  W +V  + +++     +     +++E+
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLVLLLLDITPMLPVLEGVVMELQDCALPLLREVIPTDKEDVAFKDLDAAILVGSMPRR--EGMERKDLLKANVAIFKSQGEALDKYAKKTVKVLVVGNPA----NTNCLIAAKSAPSIPKENFSCLTRLDHNRASSQVALRCGIAPNNVKNVIIWGNHSSTQYPDVHHCKVN-----VQGKDETAFDAVK---DDAWLKGEFISTVQQRGAAVIKARK-LSSAMSAAKAI*QDI--WTGTPEGEFISMGVYSTGNSYGVPEDLIYSFPISIKDKN-WKIVDGLPINDFSKAKMEATAAELVEE 320          
BLAST of Malate dehydrogenase 1B vs. Ensembl Zebrafish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 110.153 bits (274), Expect = 6.256e-26
Identity = 90/339 (26.55%), Postives = 153/339 (45.13%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++ P +V++ GA  +     + S+++GDV   +  + +V +D T  LP+L  + M   D A  L+R +  TD  E  F D    ++    +    +  E    +KAN   ++  G  +EKY    VKVL+V   A    N N +   +    IPK N   L+R    + RS VA ++ V   +V N  +WGN     Y D +++ V R      G E   ++    V D+ WLK +    +  R   +      L + ++ A AI   +     G   G   S+ I S G  YGVPD + +S PV +  +  W VV  + +++     ++    +++E+
Sbjct:    1 MAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDITPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVG--SMPRKEGMERKDLLKANVAIFKTQGEALEKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVPSDSVKNVTIWGNHSSTQYPDVHHAIVTRN-----GKE---IAAFDAVNDESWLKGDFISTVQQRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTPDGEWVSMGIYSSGNSYGVPDDLMYSFPVKIKNKS-WKVVDGLSINDFSRGKMDATAAELVEE 323          
BLAST of Malate dehydrogenase 1B vs. Ensembl Zebrafish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 52.7582 bits (125), Expect = 3.820e-8
Identity = 26/86 (30.23%), Postives = 44/86 (51.16%), Query Frame = 2
Query:  401 IREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFAD 658
            +R   A + P +V++ GA  +     + S+++GDV   +  + +V +D T  LP+L  + M   D A  L+R +  TD  E  F D
Sbjct:   12 VRTASAQAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDITPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKD 97          
BLAST of Malate dehydrogenase 1B vs. Ensembl Xenopus
Match: MDH1B (malate dehydrogenase 1B [Source:NCBI gene;Acc:100490547])

HSP 1 Score: 249.595 bits (636), Expect = 6.831e-74
Identity = 165/465 (35.48%), Postives = 253/465 (54.41%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDV----ELSEIQFKSINECIQD 1420
            MAK V+AG +DCPY+AK E+LA+ L+ N+P+F VHKI +  ++W  W  ++C  N WS   SPIIWREL+DRGGKG L+GG N+F E+ + YYN+ +D  SS M+DIAL+N+R      KE  E   L  P  V I  A      + +  L+ G+V     E+ +  ++ +     L++L M   DLA+ L+R I++    + AF  +  V++ DD   +     E    +K   E     G  I++    +VKV+   +   +Y+N+    I      +   NVV L+  +E + R+ +A+KLN+N + V + I+WGNI G  ++D  ++++++YD AV GP+ F   + K ++D+KWLKNE      +R Q      N LG  ++ A +I   L  W      G I SL I SEG + +P G+ +SMPV     G W V   V    E  E  F++  E I++
Sbjct:   13 MAKFVLAGKADCPYYAKAEILAEYLQKNLPSFRVHKITQHPEDWEEWLQKICMQNGWSHNHSPIIWRELLDRGGKGLLLGGFNDFFEHAQEYYNITTDMMSSLMKDIALENLRTHMEIKKEQEEICCLFNPLHVWITSATTPTCYNLISLLASGEVFGMRKEIWLHLLETSHCNGTLRALVMEAEDLAFPLLRKITVHKMADDAFLQADFVIVLDDVYVTNDQSPEIC--IKQIAERCLQYGALIDQNSDKEVKVV---VAGSTYVNLKAQMILSNAPSVKPHNVVALATQLEYEARAQIAKKLNINSAVVKDVILWGNISGTNFLDLQHAKIYQYDSAVWGPQGFWRPLKKMIFDRKWLKNEFLKEWRSRQQ------NRLG--MSAAHSIAMVLSWWQQESNTGDIVSLGIISEGQFELPKGIVYSMPVQF-INGEWQVYTKVTVLDETKETLFQAARELIKE 463          
BLAST of Malate dehydrogenase 1B vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 103.605 bits (257), Expect = 1.911e-23
Identity = 83/350 (23.71%), Postives = 160/350 (45.71%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWIN 1459
            L  P KV++ GA  +     +  +++GDV   +  L +V +D T  + +L+ + M   D A  L++ +  TD  + AF D  + ++     R       D     +KAN + ++  G  + KY    VKV++V   A    N N ++  +    IPK N   L+R    + +  +A KLNV   +V N I+WGN     Y D +++ V+     + G +  +    K   +  WLK E    +  R   +      L + ++ A AI   +     G   G   S+ + S+G  YG+P+ + +S P+T+  +  W +V+ + +++   + ++   +++ E+      +++
Sbjct:    7 LPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKD----LLKANVKIFKSQGAALNKYSKKSVKVIVVGNPA----NTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVN-----LQGKDVGAFEAVK---NDDWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNK-TWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLS 338          
BLAST of Malate dehydrogenase 1B vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 102.834 bits (255), Expect = 3.050e-23
Identity = 81/345 (23.48%), Postives = 159/345 (46.09%), Query Frame = 2
Query:  428 PTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWIN 1459
            P KV++ GA  +     +  +++GDV   +  L +V +D T  + +L+ + M   D A  L++ +  TD  + AF D  + ++         +  E    +KAN + ++  G  + KY    VKV++V   A    N N ++  +    IPK N   L+R    + +  +A KLNV   +V N I+WGN     Y D +++ V+     + G +  +    K   +  WLK E    +  R   +      L + ++ A AI   +     G   G   S+ + S+G  YG+P+ + +S P+T+  +  W +V+ + +++   + ++   +++ E+      +++
Sbjct:    4 PVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSM--PRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPA----NTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVN-----LQGKDVGAFEAVK---NDDWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNK-TWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLS 332          
BLAST of Malate dehydrogenase 1B vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 102.834 bits (255), Expect = 3.278e-23
Identity = 81/345 (23.48%), Postives = 159/345 (46.09%), Query Frame = 2
Query:  428 PTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWIN 1459
            P KV++ GA  +     +  +++GDV   +  L +V +D T  + +L+ + M   D A  L++ +  TD  + AF D  + ++         +  E    +KAN + ++  G  + KY    VKV++V   A    N N ++  +    IPK N   L+R    + +  +A KLNV   +V N I+WGN     Y D +++ V+     + G +  +    K   +  WLK E    +  R   +      L + ++ A AI   +     G   G   S+ + S+G  YG+P+ + +S P+T+  +  W +V+ + +++   + ++   +++ E+      +++
Sbjct:    5 PVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSM--PRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPA----NTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVN-----LQGKDVGAFEAVK---NDDWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNK-TWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLS 333          
BLAST of Malate dehydrogenase 1B vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 102.449 bits (254), Expect = 5.673e-23
Identity = 82/338 (24.26%), Postives = 156/338 (46.15%), Query Frame = 2
Query:  428 PTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            P KV++ GA  +     +  +++GDV   +  L +V +D T  + +L+ + M   D A  L++ +  TD  + AF D  + ++     R       D     +KAN + ++  G  + KY    VKV++V   A    N N ++  +    IPK N   L+R    + +  +A KLNV   +V N I+WGN     Y D +++ V+     + G +  +    K   +  WLK E    +  R   +      L + ++ A AI   +     G   G   S+ + S+G  YG+P+ + +S P+T+  +  W +V+ + +++   + ++   +++ E+
Sbjct:   20 PVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKD----LLKANVKIFKSQGAALNKYSKKSVKVIVVGNPA----NTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVN-----LQGKDVGAFEAVK---NDDWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNK-TWKIVEGLPINDFSREKMDLTAKELHEE 339          
BLAST of Malate dehydrogenase 1B vs. Ensembl Mouse
Match: Mdh1b (malate dehydrogenase 1B, NAD (soluble) [Source:MGI Symbol;Acc:MGI:1923918])

HSP 1 Score: 246.128 bits (627), Expect = 1.729e-73
Identity = 153/465 (32.90%), Postives = 252/465 (54.19%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            MAK V+AG ++CPY+AK ELLAD L+ N+P+F + KI +   +W  W  ++C+ N W  + SPIIWREL+DRGG+G L+GG NEF E+ + YY V S+ T+  M  IA +N++    +  +    K L +P +V I  A      H +  L  G+V   +TE+++   D+ +    L+S+ M T DLA  ++R++S     + AF  + +++I DD         E    +++        G  IEK     VKV+   +   +++N+    + +    I   N++ ++  VE Q ++V+ARK+    +N+ + I+WGNI G  Y+D   ++V+ Y+ AV GP     SV   ++D++W+  E     +   + +S      G  LA A +I T L  W +G   G I SL + SEG + +P+G+ FSMPV     G W V+ D+E   +  K+++    D+I++
Sbjct:    1 MAKFVIAGKANCPYYAKAELLADYLQKNLPDFRIFKITQHPDKWEDWVEDVCERNMWDHRTSPIIWRELLDRGGRGLLLGGYNEFLEHAQLYYGVTSNMTTELMMVIAKENMQTHTEQQLDKETMKDLISPLQVWIASAGTYVCCHLIPLLLSGEVFGMHTEISLTLFDQEQREDCLRSIVMETQDLASPVLRTVSFCTTVKEAFLQAQVIIILDDSTEEEVYSLESC--LRSRVPLCRLYGYLIEKNAHKSVKVI---VGGKNFVNLKTTLLMQYAPNIAS-NIIAVALGVEGQAKAVLARKMKTTSANIKDVIIWGNITGNNYVDLRKAKVYNYESAVKGPPGHYHSVLSLIFDREWITKEF----VQTLKILSSTGKQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGVMSEGQFDIPEGIVFSMPVKFE-NGTWVVLTDLEDISLSEKTLSRLTGDLIQE 453          
BLAST of Malate dehydrogenase 1B vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 115.161 bits (287), Expect = 1.739e-27
Identity = 88/352 (25.00%), Postives = 161/352 (45.74%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWINNS 1465
            +S P +V++ GA  +     + S+  G V   +  + +V +D T  + +L  + M   D A  L++ +  TD  E AF D  + V+     R       D     +KAN + ++  G  +EKY    VKV++V   A    N N ++  +    IPK N   L+R    + +S +A KL V   +V N I+WGN     Y D N+++V      + G E   + V + + D  WLK E    +  R   +      L + ++ A AI   +   W    E  ++   VI+    YGVPD + +S PV +  +  W  V+ + +++   + ++   +++ E+    + +++++
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKD----LLKANVKIFKSQGTALEKYAKKSVKVIVVGNPA----NTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVK-----LQGKE---VGVYEALKDDSWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNK-TWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA 334          
BLAST of Malate dehydrogenase 1B vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 114.775 bits (286), Expect = 1.951e-27
Identity = 88/352 (25.00%), Postives = 161/352 (45.74%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWINNS 1465
            +S P +V++ GA  +     + S+  G V   +  + +V +D T  + +L  + M   D A  L++ +  TD  E AF D  + V+     R       D     +KAN + ++  G  +EKY    VKV++V   A    N N ++  +    IPK N   L+R    + +S +A KL V   +V N I+WGN     Y D N+++V      + G E   + V + + D  WLK E    +  R   +      L + ++ A AI   +   W    E  ++   VI+    YGVPD + +S PV +  +  W  V+ + +++   + ++   +++ E+    + +++++
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKD----LLKANVKIFKSQGTALEKYAKKSVKVIVVGNPA----NTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVK-----LQGKE---VGVYEALKDDSWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNK-TWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA 334          
BLAST of Malate dehydrogenase 1B vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 114.775 bits (286), Expect = 1.951e-27
Identity = 88/352 (25.00%), Postives = 161/352 (45.74%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWINNS 1465
            +S P +V++ GA  +     + S+  G V   +  + +V +D T  + +L  + M   D A  L++ +  TD  E AF D  + V+     R       D     +KAN + ++  G  +EKY    VKV++V   A    N N ++  +    IPK N   L+R    + +S +A KL V   +V N I+WGN     Y D N+++V      + G E   + V + + D  WLK E    +  R   +      L + ++ A AI   +   W    E  ++   VI+    YGVPD + +S PV +  +  W  V+ + +++   + ++   +++ E+    + +++++
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKD----LLKANVKIFKSQGTALEKYAKKSVKVIVVGNPA----NTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVK-----LQGKE---VGVYEALKDDSWLKGEFITTVQQRGAAVIKARK-LSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNK-TWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA 334          
BLAST of Malate dehydrogenase 1B vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 56.6102 bits (135), Expect = 5.867e-9
Identity = 43/159 (27.04%), Postives = 72/159 (45.28%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFN 889
            +S P +V++ GA  +     + S+  G V   +  + +V +D T  + +L  + M   D A  L++ +  TD  E AF D  + V+     R       D     +KAN + ++  G  +EKY    VKV++V   A    N N ++  +    IPK N
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKD----LLKANVKIFKSQGTALEKYAKKSVKVIVVGNPA----NTNCLTASKSAPSIPKEN 151          
BLAST of Malate dehydrogenase 1B vs. UniProt/SwissProt
Match: sp|Q8T773|MDH1B_BRAFL (Putative malate dehydrogenase 1B OS=Branchiostoma floridae OX=7739 GN=MDH1B PE=3 SV=1)

HSP 1 Score: 341.658 bits (875), Expect = 7.725e-109
Identity = 182/485 (37.53%), Postives = 288/485 (59.38%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSE---IQFKSINECIQDIIEKTRIIYPWINNSNQSEMV 1483
            MAK V+AG +DCPY+AKVELLAD+L+  + +FHVHKIVKT +EW  W  +LC  N W+   SPIIWRELVDRGGKG LIGG N+F EY + YY + S   S  M++IA +N+   +   +E    +  S P  V ++ A      H + SL  G +L    E+A+   D  E+L  LK L+M  FDL++  ++ IS+T +   AF ++ + ++ DD +      ++ I  M+    +Y+ +   I +  S D++VL+     ++ L ++++  H     IP+ N+  +++  E+Q +S++A++L VN + V + IVWGN+GG  Y D + +RVH YDGA+ GP  +S SV++ V D KWL+ E    + +R   I D +      L++A+AI++ L  W NG   G + SL + SEG+Y +P+G+ FS PV  + +G W VVQD++++E   +   SI    Q + EK  +++P   N+N  +++
Sbjct:    1 MAKFVIAGQADCPYYAKVELLADSLQSKLQDFHVHKIVKTPEEWKPWLQQLCADNGWTHGRSPIIWRELVDRGGKGLLIGGHNDFMEYARSYYGMTSGMLSEMMKNIAAENLAVTQELREEEETIRRQSKPLHVCVINAARSPAYHVLPSLVNGKILREE-EIALHLHDSEENLEKLKGLEMEVFDLSFPFLKEISVTTDLPTAFQNAHIAIVLDDFD--QGGKEDAIGDMETKVSFYKRVAEAINQTASKDIRVLVAGTGPLNSL-VSILIDHT--PSIPRQNIAAVAQVKERQAKSLLAKRLTVNSAGVCDVIVWGNVGGTTYTDVSRARVHGYDGAIWGPPSYSCSVSEMVHDNKWLEGEFLEQLQSRSHTIQD-SLQHSADLSMAAAISSTLSYWWNGSPEGQLFSLAVCSEGFYNIPEGVVFSFPVMFH-KGSWEVVQDIDMNEDMRVMLSSIT--AQLVEEKDLLLHP--TNANHEKLL 473          
BLAST of Malate dehydrogenase 1B vs. UniProt/SwissProt
Match: sp|Q5I0G3|MDH1B_HUMAN (Putative malate dehydrogenase 1B OS=Homo sapiens OX=9606 GN=MDH1B PE=2 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 2.343e-80
Identity = 167/465 (35.91%), Postives = 260/465 (55.91%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            MAK V+AG +DCPY+AK EL+AD L+ N+P+F +HKI +  + W  W  ++C+ N WS K+SPIIWREL+DRGGKG L+GG NEF E+ + YY+V S  T+  M  IA +N+     +++E    K    P +V I  A   A  + +  L+ G+V   +TE++I   D  +    LKSL + T DLA  ++RS+SI    E AF  + ++V+ DD         E    +++        G  IEK     V+V+   +   +++N+  + + R   RI   N++ ++  VE + ++++ARKL   PS + + I+WGNI G  Y+D   +RV+RY+ A+ GP  +S  V   ++D +W+K E   ++ N    ++      G  LA A +I T L  W +G   G I SL I SEG +G+P G+ FSMPV     G W V+ D++  EI  + +     D+I++
Sbjct:    1 MAKFVIAGRADCPYYAKTELVADYLQKNLPDFRIHKITQRPEVWEDWLKDVCEKNKWSHKNSPIIWRELLDRGGKGLLLGGYNEFLEHAQLYYDVTSSMTTELMMVIAQENLGAHIEKEQEEEALKTCINPLQVWITSASAPACYNLIPILTSGEVFGMHTEISITLFDNKQAEEHLKSLVVETQDLASPVLRSVSICTKVEEAFRQAHVIVVLDDSTNKEVFTLE--DCLRSRVPLCRLYGYLIEKNAHESVRVI---VGGRTFVNLKTVLLMRYAPRIAH-NIIAVALGVEGEAKAILARKLKTAPSYIKDVIIWGNISGNNYVDLRKTRVYRYESAIWGPLHYSRPVLNLIFDSEWVKREFVAILKN----LTTTGRQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGILSEGQFGIPKGIVFSMPVKFE-NGTWVVLTDLKDVEISEQIMTRMTSDLIQE 453          
BLAST of Malate dehydrogenase 1B vs. UniProt/SwissProt
Match: sp|Q08BZ4|MDH1B_DANRE (Putative malate dehydrogenase 1B OS=Danio rerio OX=7955 GN=mdh1b PE=2 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 7.736e-77
Identity = 153/463 (33.05%), Postives = 243/463 (52.48%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKE-NEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDI 1423
            MAK V+AG +DCPY+AK ELLAD L+ N+P+FH+HKI     +W  W  + C +N W    SP++WREL+DRGGKG L+GG ++F E+ + YY + SD  S  M  IA +N   KE   ++E+   K+L  P  + I  A+N      +  L    + +    L++  +D +    +L++L+M T DLA   +   +   N              D++N    D D+         E++   G  IE     DV+VL   +    ++N+    +      I   N V ++  +E + R+ +A+KL+V  S++ N IVWGNI G  +ID   ++V RYDGA+ GP+ FS  V + ++D KWLK +   L+      IS   N   T ++  +AI   L  W N +    + SL + S G +G+P G+ FSMPV+    G+W+V  DV +++    +++ C  ++
Sbjct:    1 MAKFVLAGKADCPYYAKAELLADVLQRNLPDFHIHKIPVPPDDWKEWLEDTCASNGWKHSSSPLVWRELIDRGGKGMLLGGFSDFLEHAQGYYGITSDMKSDLMLKIAEENQATKELCMEEEVHRLKSLR-PLHIWISSALNPVCYSLIPHLFTPGLFSGLPILSLHLMDTSGSEEMLQALKMETVDLAIPRLHEPAGEYN--------------DEQN----DKDQV-------AEHFHRYGQLIETNAQKDVRVL---VAGDFFINMKCSLLIENAPSIDSRNFVAMTTQLEYEARTQLAQKLSVKTSDITNVIVWGNISGSFHIDLQRAKVFRYDGAIRGPDGFSQHVMEMIYDWKWLKTDFRSLLHKHRATISSKTNK-ATAISTTNAILAILKAWNNAESPEEVFSLGVLSTGQFGIPAGLVFSMPVSFT-DGHWSVRSDVTVTDELRVNLDACADEL 432          
BLAST of Malate dehydrogenase 1B vs. UniProt/SwissProt
Match: sp|A3KMX7|MDH1B_BOVIN (Putative malate dehydrogenase 1B OS=Bos taurus OX=9913 GN=MDH1B PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 5.663e-75
Identity = 165/487 (33.88%), Postives = 261/487 (53.59%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKF--NVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWINNSN--QSEMVI 1486
            MAK V+AG +DCPY+AK ELLAD L+ N+P+F +HKI +    W  W  +LCK N WS   SPIIWREL+DRGGKG L+GG NEF E+ + YY V S  T+  M+ +A +N+     ++ E    K L  P +V I  A + A  H +  L+ G+V     E++I   D       L S +    DLA   ++S+SI    E AF  + +++I DD      +  E    +++     +  G+ IEK      K++   +   +++N+       +++  P F  N++ ++  VE Q ++ +ARKL + PS + + I+WGNI G  Y+D   ++V+RY+ A+ GP  +S  V   ++D++W+  E     +   + ++      G  LA A +I T L  W +G   G I SL + SEG +G+P G+ FSMPV     G W V+ D++ +EI  + +     D+I++  +    + N    QSE +I
Sbjct:    1 MAKFVLAGRADCPYYAKAELLADYLQKNLPDFRIHKITQHPDIWEEWLKDLCKKNRWSHNKSPIIWRELLDRGGKGLLLGGYNEFLEHAQLYYGVTSSMTTELMKRVAQENLGTHIEKELEKESLKGLVNPLQVWITSASSLACYHLIPILTSGEVFGPQMEISINLFDNKHTEEKLISHKQEAEDLASPCLQSVSICTQAEEAFHGAHVIIILDDHVDKEINSLE--DCIRSRAPLCQLYGSLIEKNAHNFAKII---VGGKTFVNLK---TALLMKYAPTFAHNIIAVALGVEGQAKAALARKLKITPSCIKDVIIWGNISGNNYVDLRKAKVYRYESAIWGPPHYSRPVLSLLFDREWVNREF----VVSLKTLTATGRQFGGMLA-AHSIATTLKYWCHGSPPGEIVSLGVLSEGQFGIPKGIVFSMPVKFE-NGTWVVLTDLKNTEISQQVMTRMANDLIQEQLVALGELANFQPYQSENLI 473          
BLAST of Malate dehydrogenase 1B vs. UniProt/SwissProt
Match: sp|Q5F204|MDH1B_MOUSE (Putative malate dehydrogenase 1B OS=Mus musculus OX=10090 GN=Mdh1b PE=2 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 1.209e-72
Identity = 153/465 (32.90%), Postives = 252/465 (54.19%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            MAK V+AG ++CPY+AK ELLAD L+ N+P+F + KI +   +W  W  ++C+ N W  + SPIIWREL+DRGG+G L+GG NEF E+ + YY V S+ T+  M  IA +N++    +  +    K L +P +V I  A      H +  L  G+V   +TE+++   D+ +    L+S+ M T DLA  ++R++S     + AF  + +++I DD         E    +++        G  IEK     VKV+   +   +++N+    + +    I   N++ ++  VE Q ++V+ARK+    +N+ + I+WGNI G  Y+D   ++V+ Y+ AV GP     SV   ++D++W+  E     +   + +S      G  LA A +I T L  W +G   G I SL + SEG + +P+G+ FSMPV     G W V+ D+E   +  K+++    D+I++
Sbjct:    1 MAKFVIAGKANCPYYAKAELLADYLQKNLPDFRIFKITQHPDKWEDWVEDVCERNMWDHRTSPIIWRELLDRGGRGLLLGGYNEFLEHAQLYYGVTSNMTTELMMVIAKENMQTHTEQQLDKETMKDLISPLQVWIASAGTYVCCHLIPLLLSGEVFGMHTEISLTLFDQEQREDCLRSIVMETQDLASPVLRTVSFCTTVKEAFLQAQVIIILDDSTEEEVYSLESC--LRSRVPLCRLYGYLIEKNAHKSVKVI---VGGKNFVNLKTTLLMQYAPNIAS-NIIAVALGVEGQAKAVLARKMKTTSANIKDVIIWGNITGNNYVDLRKAKVYNYESAVKGPPGHYHSVLSLIFDREWITKEF----VQTLKILSSTGKQFGGILA-AHSIATTLKYWYHGSPPGEIVSLGVMSEGQFDIPEGIVFSMPVKFE-NGTWVVLTDLEDISLSEKTLSRLTGDLIQE 453          
BLAST of Malate dehydrogenase 1B vs. TrEMBL
Match: K1QKI3 (Putative malate dehydrogenase 1B OS=Crassostrea gigas OX=29159 GN=CGI_10018565 PE=4 SV=1)

HSP 1 Score: 372.474 bits (955), Expect = 4.580e-118
Identity = 196/465 (42.15%), Postives = 276/465 (59.35%), Query Frame = 2
Query:   59 GCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDR-NPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDV-ELSEIQFKSINECIQDIIEKTRIIY 1447
            G SDCPY+A+ ELL D L  N+P+F +HKIV   +EW  W  E C +  WS    P++WREL+DRGGKG LIGGANEFQEY   YY V S   S  M+ +A +N  +K  EDKE    KALS P  V I  A N+   + + +++RGD+    TE+++  +D   ++  L+ L+M   DLA+ L+R I +T +   AF     +++ DD         D+++   K + E++      I +    +VKVLL         N+N+  + +    IPK N+VGLSRT+E   +SVV  KLNVNPS V + IVWGNI G  YID +  RVH YDGA+ GP  FSL   + +WDKKW++NE   L+  R Q+  +L     + ++ A+AI T +  W NG   G I SLV+ASEGWY VP+ +  S PVT++P+G W VVQD+ + SE++ K I E ++DII    +I+
Sbjct:   10 GKSDCPYYARSELLGDKLVKNLPDFQLHKIVIKPEEWDKWLKETCSSREWSHNKCPLVWRELIDRGGKGVLIGGANEFQEYAFGYYGVQSTQVSDDMRKMAEENKAYKIEEDKEEEAIKALSHPLNVCITNASNQVCYNLINAMARGDIFGETTEISLHLLDDKANMESLEGLRMEAMDLAHGLLREIIVTSDVSKAFQGCDAIIVMDDLVQKEKESRDDFV---KRSVEHHVKYAKVINEKAKKEVKVLLT---GTGPTNLNIYMMIQNAPNIPKQNIVGLSRTLENNAKSVVGEKLNVNPSGVVDLIVWGNINGGTYIDLSKCRVHGYDGAIWGPPSFSLPAVEMIWDKKWMENEFTELVKGRHQKSEELMKHSAS-MSTAAAIETTMAHWWNGTPEGQIFSLVVASEGWYEVPEELVCSFPVTISPKGNWCVVQDINQTSEVKEK-IKEAVKDIIADKNVIF 466          
BLAST of Malate dehydrogenase 1B vs. TrEMBL
Match: A0A1S3J549 (putative malate dehydrogenase 1B OS=Lingula unguis OX=7574 GN=LOC106170262 PE=4 SV=1)

HSP 1 Score: 368.622 bits (945), Expect = 2.134e-116
Identity = 184/469 (39.23%), Postives = 279/469 (59.49%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRII 1444
            MAKIV+AG ++CPYFA+ ELLAD L  N+ +F +HKIV    +W  W    CK   +    SP++WRELVDRGGKG LIGGAN+FQEY   YY + SDF S  M+ I  +N++ K   DKE  EF++ S P KV I  A  +   H + S+ +GDV    TE+++  +D  E++  L+ +QM   DLA+ L+R +S+  N   AF D S ++  DD   S  D +    W K N + +   G+ +++    + +VL   +     +N N   +H+ + +IP+ N+V LSR VE   +++++ +L VN ++V + IVWGN+GG  Y D + +R H YDGA+ GP  FS S  + V D KWL+ E    +  R + +        + L+ A+A+ + L  W NG   G + S+ +ASEGWYGVP+G+ FS P T++P+GYW+V+QD+ELS+     I   ++++I +  +I
Sbjct:    1 MAKIVLAGAANCPYFARCELLADTLADNLEDFKLHKIVVQPDKWQNWLESTCKERGFMHSSSPLVWRELVDRGGKGVLIGGANDFQEYAYGYYGIKSDFVSDDMKKIGEENLKVKIEVDKEEEEFRSRSRPLKVCITNASCDVCYHMISSIGQGDVFGKGTEISLHLLDTAENMEYLEGVQMEAIDLAHGLLRKVSVMTNPREAFMDCSAIIFLDDIVKS--DEESKDDWYKRNADCFTNYGHVLDEVAKKNARVL---VAGEGPVNFNTFILHQHITQIPRQNIVALSRMVENHAKAIISERLKVNSADVVDIIVWGNVGGTSYADMSLARTHNYDGAIWGPPFFSRSAVELVHDNKWLEKEFPEELAARGEMVGKALRKKPS-LSKAAAVVSQLAHWWNGSPSGQMWSMGVASEGWYGVPEGLVFSFPTTMSPKGYWSVIQDIELSDETKDKIQTIVKELISEKEVI 463          
BLAST of Malate dehydrogenase 1B vs. TrEMBL
Match: A0A1S3H2A8 (putative malate dehydrogenase 1B OS=Lingula unguis OX=7574 GN=LOC106151071 PE=4 SV=1)

HSP 1 Score: 368.237 bits (944), Expect = 3.766e-116
Identity = 184/469 (39.23%), Postives = 280/469 (59.70%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRII 1444
            MAKIV+AG ++CPYFA+ ELLAD L  N+ +F +HKIV    +W  W    CK   +    SP++WRELVDRGGKG LIGGAN+FQEY   YY + SDF S  M+ I  +N++ K   DKE  EF++ S P KV I  A  +   H + S+ +GDV    TE+++  +D  E++  L+ +QM   DLA+ L+R +S+  N + AF D S ++  DD   S  D +    W K N + +   G+ +++    + +VL   +     +N N   +H+ + +IP+ N+V LSR VE   +++++ +L VN ++V + IVWGN+GG  Y D + +R H YDGA+ GP  FS S  + V D KWL+ E    +  R + +        + L+ A+A+ + L  W NG   G + S+ +ASEGWYGVP+G+ FS P T++P+GYW+V+QD+ELS+     I   ++++I +  +I
Sbjct:    1 MAKIVLAGAANCPYFARCELLADTLADNLEDFKLHKIVVQPDKWQNWLESTCKERGFIHGSSPLVWRELVDRGGKGVLIGGANDFQEYAYGYYGIKSDFVSDDMKKIGEENLKVKIEVDKEEEEFRSRSRPLKVCITNASCDVCYHMISSIGQGDVFGKGTEISLHLLDTAENMEYLEGVQMEAIDLAHGLLRKVSVMTNPKEAFMDCSAIIFLDDIVKS--DEESKDDWYKRNADCFTNYGHVLDEVAKKNARVL---VAGEGPVNFNTFILHQHITQIPRQNIVALSRMVENHAKAIISERLKVNSADVVDIIVWGNVGGTSYADMSLARTHNYDGAIWGPPFFSRSAVELVHDNKWLEKEFPEELAARGEMVGKALRKKPS-LSKAAAVVSQLAHWWNGSPSGQMWSMGVASEGWYGVPEGLVFSFPTTMSPKGYWSVIQDIELSDETKDKIQTIVKELISEKEVI 463          
BLAST of Malate dehydrogenase 1B vs. TrEMBL
Match: A0A2C9JX51 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106071519 PE=4 SV=1)

HSP 1 Score: 361.303 bits (926), Expect = 1.676e-113
Identity = 195/472 (41.31%), Postives = 273/472 (57.84%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAI-VFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRII 1444
            MAKIV+AG SDCPYFA+VELL D L  N+  F +HK++    +W  W    C    W    SPIIWREL+DRGGKG LIGGANEFQEY+K YY+V S+ TS  M+ +A +N + K+  D+E + FK+LS P  V I  A +    H + S+S G VL    EL I + V+  E L  +    M   DLA  L+R + +    E AF D  +++  D+  R         YI +M      + + G  I+K    + KVL+     I   N N   + R  Q+I + NVV ++  VE Q +SV+  KLNVNP+ V N +VWGNI  K ++D +  +VH YDGA+ GP  +S++  + + D KWL +E   L+  R  ++S +  G  + L+ A++I+T L  W  G  HG I SL + SEGWYGVP+G+ FS PVT++P+GYW VVQDV L+     +I   I+D+  +  II
Sbjct:    1 MAKIVIAGRSDCPYFARVELLGDRLAKNLKKFKLHKLMIQPDKWDEWLHTTCAERGWEFHSSPIIWRELIDRGGKGVLIGGANEFQEYVKAYYDVESEMTSYEMRMVAAENKKTKDELDEEEKYFKSLSNPVNVCITNAASPVCYHLLHSISTGQVLGPKVELVIHLLVNSEEDLEQVMGTAMEAEDLALSLLRDVKVFTEAEEAFKDCQIIIFLDEIQRQEDMTMRKWYIQYMA----LFTFYGEIIDKKALRNCKVLVCGNGPI---NFNATILARSAQKIARQNVVAVASVVENQAKSVIGEKLNVNPAGVVNLLVWGNINAKRHLDVSKCQVHGYDGAITGPSWYSVNAIEMLHDNKWLSSEFPALVSARHLKVSRV-MGRSSSLSTANSISTLLKLWFRGSPHGQIFSLAVYSEGWYGVPEGIFFSFPVTMDPKGYWHVVQDVFLTLETKLNIKSSIEDLQYELGII 464          
BLAST of Malate dehydrogenase 1B vs. TrEMBL
Match: A0A210Q1L4 (Malate dehydrogenase 1B OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT04875 PE=4 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 1.056e-112
Identity = 195/472 (41.31%), Postives = 282/472 (59.75%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD--RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIY 1447
            MAK+V+AG +DCPY+A+ ELL D L  N+P+F +HKIVK   EW  W    C    W+   SPI+WREL+DRGGKG LIGGAN+FQEY   YY + S+  SS M  I+ +N+++K+  D+E +EFKA S P  V +  A N A    V ++S+GDV  S  E+A+   D  + + +L+ L+M   DLA+ L+R +++  N   AF++ S +++ D+  RN    D D    W K N   +      I      +VKVLL     +   N N+  + +    IP+ N  GLSR +E   ++V+A KL VN S V N ++WGN+ G+ YID   SRVH Y+GA+ GP  FSL   + +WDK+WL  E   L+  R   + +  N  G  ++  +AI T LG W  G   G I SL + SEGWYGVPDG+ +S PVT++P+GYW VV+D++L+E   +++   +QD+I +  I+Y
Sbjct:    1 MAKVVIAGKADCPYYARCELLGDKLAKNLPDFCLHKIVKQNDEWEKWLKATCNERGWTHMKSPIVWRELIDRGGKGVLIGGANDFQEYAHGYYGMESELLSSDMLKISKENLQYKKEVDEEEKEFKAQSKPLHVCLTVASNPACYAMVNAISKGDVFGSKQEVALHLYDANDKMEMLEGLRMEAIDLAHGLLRDVTVESNLCKAFSNCSAIILMDEVVRNEGE-DKD---SWYKRNSNLFCNYAKIINDVARKNVKVLLTGDGPV---NFNVFMMLQNAPNIPRQNFAGLSRMLENHTKAVIADKLKVNSSGVVNVVIWGNVSGEYYIDLETSRVHGYEGAIWGPPSFSLPTKEMIWDKQWLAKEFPELVKARKAMVEEALNH-GAYMSKGAAIATMLGHWWTGSPSGEIFSLAVCSEGWYGVPDGLVYSFPVTMHPKGYWYVVEDIDLTEEVKETLKVTVQDLISEKEIMY 464          
BLAST of Malate dehydrogenase 1B vs. Ensembl Cavefish
Match: mdh1b (malate dehydrogenase 1B [Source:NCBI gene;Acc:103037214])

HSP 1 Score: 248.054 bits (632), Expect = 1.384e-74
Identity = 147/467 (31.48%), Postives = 247/467 (52.89%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKE-IREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDD---RNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELS-EIQFK---SINECI 1414
            MAK V+AG +DCP++AK ELLAD L+  +P+FH+HKI     +W  W    C +N W  + SPI+WREL+DRGGKG L+GG ++F E+++ YY + SD  S  M  IA +N+R  E   +E  R+ ++L     + I GA++      +  L   +++   + +++  +D       L  L+M T DLA   +  +++  +   AF  + L++  DD    + +P +  +    +    E +   G  +E     DV+V+   +   SY+N+    +      +     V ++  +E + R+ +A KL+V  +++ + IVWGNI G  ++D   ++V RY G++ GP  FS  V + ++DKKWL+ +   L+  R + IS   N      A  + I   L  W N      + SL + S G +GVP G+ FS+PV  +  G W+V  D+ ++ E++ K   +INE +
Sbjct:    1 MAKFVLAGRADCPHYAKTELLADLLQKILPDFHIHKICVHPSDWQDWLEGTCSSNGWKHESSPIVWRELIDRGGKGMLLGGFSDFLEHVQGYYGITSDMGSEQMMKIAAENLRVTEVLMEEAARQNQSLQV-FHIWITGALSPTCYSLIPKLFTTELIRDISTISLHLLDVNSSEESLLGLKMETEDLALSQLHEVTVHSDMTWAFQSADLILFLDDWPSNSETPVEQGDKDHMVSQVAERFRSYGRLVEANAQKDVRVM---VAGDSYVNLKCSLLIENAPSVDPCRFVAMTTQLEYEARAQLAEKLSVKTTDITDIIVWGNISGSSHVDLQRAKVFRYKGSIWGPTGFSQQVLEMIYDKKWLETDFLSLVALRRRAISLKTNRTAANSA-TNGILAVLNAWSNCYSSREVFSLGVVSSGNFGVPAGLVFSVPVKFH-NGDWSVCSDITINDELRSKLGVAINELL 461          
BLAST of Malate dehydrogenase 1B vs. Ensembl Cavefish
Match: mdh1b (malate dehydrogenase 1B [Source:NCBI gene;Acc:103037214])

HSP 1 Score: 228.024 bits (580), Expect = 2.313e-67
Identity = 143/464 (30.82%), Postives = 234/464 (50.43%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKE-IREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELS-EIQFK---SINECI 1414
            MAK V+AG +DCP++AK ELLAD L+  +P+FH+HKI     +W  W    C +N W  + SPI+WREL+DRGGKG L+GG ++F E+++ YY + SD  S  M  IA +N+R  E   +E  R+ ++L     + I GA++      +  L   +++   + +++  +D       L  L+M T DLA   +    +                         D D  +  +    E +   G  +E     DV+V+   +   SY+N+    +      +     V ++  +E + R+ +A KL+V  +++ + IVWGNI G  ++D   ++V RY G++ GP  FS  V + ++DKKWL+ +   L+  R + IS   N      A  + I   L  W N      + SL + S G +GVP G+ FS+PV  +  G W+V  D+ ++ E++ K   +INE +
Sbjct:    1 MAKFVLAGRADCPHYAKTELLADLLQKILPDFHIHKICVHPSDWQDWLEGTCSSNGWKHESSPIVWRELIDRGGKGMLLGGFSDFLEHVQGYYGITSDMGSEQMMKIAAENLRVTEVLMEEAARQNQSLQV-FHIWITGALSPTCYSLIPKLFTTELIRDISTISLHLLDVNSSEESLLGLKMETEDLALSQLHETPV----------------------EQGDKDHMVSQVA---ERFRSYGRLVEANAQKDVRVM---VAGDSYVNLKCSLLIENAPSVDPCRFVAMTTQLEYEARAQLAEKLSVKTTDITDIIVWGNISGSSHVDLQRAKVFRYKGSIWGPTGFSQQVLEMIYDKKWLETDFLSLVALRRRAISLKTNRTAANSA-TNGILAVLNAWSNCYSSREVFSLGVVSSGNFGVPAGLVFSVPVKFH-NGDWSVCSDITINDELRSKLGVAINELL 433          
BLAST of Malate dehydrogenase 1B vs. Ensembl Cavefish
Match: mdh1aa (malate dehydrogenase, cytoplasmic [Source:NCBI gene;Acc:103036835])

HSP 1 Score: 109.383 bits (272), Expect = 1.404e-25
Identity = 88/345 (25.51%), Postives = 153/345 (44.35%), Query Frame = 2
Query:  401 IREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            +R   A + P +V++ GA  +     + S+++GDV   +  + +V +D    LP+L  + M   D A  L+R +  TD  E  F D    ++         +  E    +KAN   ++  G  +EKY    VKVL+V   A    N N +   +    IPK N   L+R    + RS VA ++ V+   V N I+WGN     Y D +++ V+++     G E   +     V D  WLK +    +  R   +      L + ++ A AI   +   W    +  ++   V +S   YGV D + +S PV +  +  W VV  + +++     ++    ++IE+
Sbjct:   12 VRAASAQAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRK--EGMERKDLLKANVAIFKTQGAALEKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVSSDKVKNVIIWGNHSSTQYPDVHHATVNQH-----GKE---VPAYDAVKDDSWLKADFISTVQQRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTPDGEFVSMGVYSSGNSYGVADDLMYSFPVMIKNKS-WKVVDGLPINDFSRGKMDATAAELIEE 340          
BLAST of Malate dehydrogenase 1B vs. Ensembl Cavefish
Match: mdh1ab (malate dehydrogenase, cytoplasmic [Source:NCBI gene;Acc:103033607])

HSP 1 Score: 108.227 bits (269), Expect = 2.614e-25
Identity = 86/339 (25.37%), Postives = 153/339 (45.13%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++   +V++ GA  +     + S+++GDV   +  L +V +D    LP+L+ + M   D A  +++ +  TD  E AF D    ++         +  E    +KAN   ++  G  ++KY    VKVL+V   A    N N +   +    IPK N   L+R    + RS VA +  +   NV N I+WGN     Y D ++  V     +V G +  +  + K   D  WLK +    +  R   +      L + ++ A AI   +    NG   G   S+ + S G  YGVP+ + +S PVT+  +  W +V  + +++     ++    +++E+
Sbjct:    1 MAETIRVLVTGAAGQIAYSLLYSIAKGDVFGRDQPLVLVLLDIPPMLPVLEGVVMELQDCALPILKEVIPTDKEEVAFKDLDAAILVGSMPRK--EGMERKDLLKANVAIFKSQGAALDKYAKKTVKVLVVGNPA----NTNCLVAAKSAPSIPKENFSCLTRLDHNRARSQVAMRCGIPADNVKNVIIWGNHSSTQYPDVHHCMV-----SVGGKDVAAFDMVK---DDDWLKGDFISTVQQRGAAVIKARK-LSSAMSAAKAICDHMRDIWNGTPEGEFISMGVYSTGNPYGVPEDLIYSFPVTIKDKS-WKIVDGLAINDFSRAKMDATAAELVEE 323          
BLAST of Malate dehydrogenase 1B vs. Ensembl Cavefish
Match: mdh1aa (malate dehydrogenase, cytoplasmic [Source:NCBI gene;Acc:103036835])

HSP 1 Score: 107.842 bits (268), Expect = 3.247e-25
Identity = 86/339 (25.37%), Postives = 151/339 (44.54%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++ P +V++ GA  +     + S+++GDV   +  + +V +D    LP+L  + M   D A  L+R +  TD  E  F D    ++         +  E    +KAN   ++  G  +EKY    VKVL+V   A    N N +   +    IPK N   L+R    + RS VA ++ V+   V N I+WGN     Y D +++ V+++     G E   +     V D  WLK +    +  R   +      L + ++ A AI   +   W    +  ++   V +S   YGV D + +S PV +  +  W VV  + +++     ++    ++IE+
Sbjct:    1 MAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRK--EGMERKDLLKANVAIFKTQGAALEKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVSSDKVKNVIIWGNHSSTQYPDVHHATVNQH-----GKE---VPAYDAVKDDSWLKADFISTVQQRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTPDGEFVSMGVYSSGNSYGVADDLMYSFPVMIKNKS-WKVVDGLPINDFSRGKMDATAAELIEE 323          
BLAST of Malate dehydrogenase 1B vs. Ensembl Sea Lamprey
Match: mdh1b (malate dehydrogenase 1B, NAD (soluble) [Source:NCBI gene;Acc:767794])

HSP 1 Score: 254.988 bits (650), Expect = 5.208e-78
Identity = 153/458 (33.41%), Postives = 246/458 (53.71%), Query Frame = 2
Query:   56 AGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIE 1429
            +G +DCPY+AK ELL D L++N+P+F VHKI    +EW AW S  C+ + W    SP++WRELV+RGG+G L+GG N+F EY++ YY ++S   S  M  +A +N+  +     E  + + LS P  V I GA +  VP  +  L  G++   +T L++  + K      L+ ++  T D+A   +R ++I  + E AF  + LV++ D  N +P    E   W  A        G+ + +    DV+VL+   K  + L   L++       +P+ N V LS  +E++ + ++A KL VN + V + +VWG+ GG  Y   + +R+  YDGAV GP   +  + + + D++WL+NE  P  L     +     G     AL +AI + L  W +G +   IHSL + S G YG+  G+ FS+PV     G W+VV D+ L++ +   I+    ++ E
Sbjct:    8 SGRADCPYYAKAELLTDYLQLNLPSFRVHKIPVLPEEWEAWLSATCRQHGWRHTTSPLVWRELVERGGRGALLGGYNDFMEYVQGYYGMVSPMLSELMLTLAQENLHTQVKLTDEQAQHRELSRPFHVCITGANSACVPGLLWLLGTGELCGGSTGLSVCLLGKVGEQEALRCVRQDTLDMACAALRDVTIHSDPELAFDGAGLVIVLD--NVAPGLGQE---WSDAGAPLCAAYGSALGRRAGPDVRVLVAAAKGAN-LRAQLLATAAA-PHVPRRNFVALSTHLERRAKGLLAAKLKVNSAGVEDVVVWGDAGGSTYTQLSAARLRGYDGAVWGPG-LTYPLLEVLNDREWLENEF-PAALQARGSLQAKAQGYPATTALNAAIASTLRLWFHGSDDQQIHSLAVPSNGEYGLLQGLVFSLPVRFE-GGDWSVVSDLSLTDAERSRIHAIAAELQE 455          
BLAST of Malate dehydrogenase 1B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007050.1 (pep scaffold:Pmarinus_7.0:GL479360:144570:153551:-1 gene:ENSPMAG00000006369.1 transcript:ENSPMAT00000007050.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 2.781e-8
Identity = 44/165 (26.67%), Postives = 77/165 (46.67%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFAD--SSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSR 907
            ++   KV++ GA  +     + S++ G+V   +  +++V +D T  L +L+ + M   D A  L++ +  TD  E AF D  ++++V    R       D     +KAN   ++  G  + KY    VKVL+V   A    N N +   +    IP+ N   L+R
Sbjct:    1 MTGTVKVLVTGAAGQIAYSLLYSIASGNVFGKDQPISLVLLDITPMLGVLEGVVMELQDCALPLLKEVIATDKEEVAFKDLDAAILVGSMPRREGMERKD----LLKANVRIFKSQGAALNKYSKKTVKVLVVGNPA----NTNCLIALKSALTIPRENFTCLTR 157          
BLAST of Malate dehydrogenase 1B vs. Ensembl Nematostella
Match: EDO45840 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRY2])

HSP 1 Score: 272.322 bits (695), Expect = 2.453e-84
Identity = 165/470 (35.11%), Postives = 253/470 (53.83%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPA-DPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPE--PFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTR 1438
            MAK VV+G SDCP++AK ELLAD L +N+P+F VHKIVK   EW  W +++C  N W    SP+IWRELVDRGGKG L+GG ++F E+ + YY + S   S+ +  I+ +N   K   DKE  E      P +V +  A        + SL +G+V     E++I  +D  E+   L+ +     D A+ L R + IT +   AF D+S+VV+ D +  +   D  EY   + ++ + +   G  +E +   D KVL     A    N +     +    I K N V LSR  E + + ++A++LNVN + + + IVWGN G   Y D +++RV  +DGA+ GP    F+  V + V D KWL  E     +   ++ S+  N L   +A+ S +  +  Q  + D    + SL + SEGWY +P+G+AFS PV     G W VV+ + LS+    S+ E +++I ++ +
Sbjct:    1 MAKFVVSGRSDCPFYAKAELLADELSLNLPDFKVHKIVKRPDEWDDWMNKMCDDNGWKHSGSPLIWRELVDRGGKGILLGGCDDFLEFAEGYYGLASRLKSAKLVSISEENKSTKIEVDKEEEELAKKINPLRVCVSKASGPLAYGMLASLVQGEVFGFQEEVSIYLLDTPENQEALQGVAYEIEDCAWPLFRGVHITSDPAVAFKDASVVVLLDGKAINEGTDKKEY---LLSHAKLFRDYGKALEAHAKPDCKVLTAGGPA----NFSTFIASKFAPSIQKKNFVSLSRIEENRAKGLIAKRLNVNTAGIKDLIVWGNPGFNHYPDASHARVDGFDGAIWGPHVPGFTRPVPEMVHDNKWLSGEF----VEVLKKPSEGENALMGSVAVKSLLQDWC-QCASSD----MWSLGVISEGWYDIPEGIAFSFPVKFT-GGSWQVVEGLALSD----SLKEVLKNIAQEAK 449          
BLAST of Malate dehydrogenase 1B vs. Ensembl Nematostella
Match: EDO41218 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4V3])

HSP 1 Score: 106.686 bits (265), Expect = 3.071e-25
Identity = 84/330 (25.45%), Postives = 148/330 (44.85%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSP-ADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSIN 1405
            ++ P +V I GA  +     + S+++GDV  +N  + +  +D T  +  L  + +   D A  L+  +  TD+ E AF D  + ++       P  +  E    + AN + +E  G  ++K      K+L+V   A    N N +   R    IPK N   L+R  + +  S +A +LN+    V N I+WGN     Y D N+ +V      V G     +++   V D  +L  E    +  R   I      L + ++ A AI   +  W  G   G   S+ + S+G YG+ +G+ +S PV +  +  W +VQ + +++   + +N
Sbjct:    1 MAEPLRVCITGAAGQIAYSLLYSVAKGDVFGANQPIILHLLDITPMMGALGGVVLELQDCAIPLLHEVVATDDAEKAFTDIDVAILV---GAMPRREGMERKDLLSANAKIFEAQGKALDKVAKKTCKILVVGNPA----NTNCLIAQRWAPSIPKENFSCLTRLDQNRGGSQIAGRLNLRVDQVKNVIIWGNHSATQYPDVNHGKV-----CVNGNW---VAIRDAVKDDDYLNGEFISTVQKRGAAIIKARK-LSSAMSAAKAICDHMRDWWFGTREGEWVSMGVVSDGSYGIEEGLVYSFPVQICDR-TWKIVQGLSINDFSREKMN 313          
BLAST of Malate dehydrogenase 1B vs. Ensembl Medaka
Match: mdh1b (malate dehydrogenase 1B [Source:NCBI gene;Acc:101155092])

HSP 1 Score: 201.06 bits (510), Expect = 4.712e-57
Identity = 137/466 (29.40%), Postives = 230/466 (49.36%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQE----YYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDI 1423
            MAK VVAG +DCP++A+ E LAD L+ ++PNF  HKI     EW  W    C  N W  ++SP++WRELV++GGKG L+GG ++F  + + YY V SD     M  +A +N+  +    +E ++   L  P  V I GA +      + +L   ++      +++  +D  E+   L+ L+  T  LA  ++R +++  + + AF ++ ++V+ DD N       E     K   +     Y      I+   +  VKV+   +   S++N+    +      I     V ++  +E + R+VVA KL V P+ V   IVWGNI G  YID   ++V  + G + GP  F   +++ + D+KWL+  ++ L+  R   ++    G     + AS I   L  W  G     I S  +   G + + D + FS+PVT    G W++V DV + +   + +  C  ++
Sbjct:    1 MAKFVVAGKADCPFYAEAERLADTLQRSLPNFRAHKICVLPDEWEGWLDGTCSKNGWKHEESPLVWRELVEQGGKGLLLGGLSDFLNHCQDYYGVSSDVNPDMMLSVAAENLETQIKLLQEEQDHLNLVKPIHVWITGAASHTSRILIPNLLSAEIFPKAPAVSLHLLDLDENEEELQRLREETQSLALSMLRRVTVHTDPQQAFREADVIVLLDDTNGGRRGEGEEEEEEKQEVKSVCNRYREFSRLIDAKANKQVKVI---VSGDSHVNLRCSLLVEGAHSIDSHQFVAVATQLENEARAVVAEKLGVRPTEVTEVIVWGNISGLSYIDLQRAKVFNFQGPIKGPAFFPQPISRILPDRKWLETNLQDLVRRRRDAVTS-RTGQAASASAASGILRVLKAWHAGGGPDEIMSAGVHCSGLFNLRDDIIFSVPVTFEG-GKWSMVLDVTVGDGLMRRLELCADEL 461          
BLAST of Malate dehydrogenase 1B vs. Ensembl Medaka
Match: mdhb (cytosolic malate dehydrogenase B [Source:NCBI gene;Acc:100049475])

HSP 1 Score: 108.227 bits (269), Expect = 2.698e-25
Identity = 84/339 (24.78%), Postives = 149/339 (43.95%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEG-WYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++ P  V++ GA  +     + S+++GDV   +  + ++ +D T  LP+L+ + M   D A  L+R +  TD  E AF D    ++         +  E    +KAN   +   G  ++KY    V VL+V   A    N N +   +    IPK N   L+R    +  S VA +  V  ++V N I+WGN     Y D ++ +V    G         L+    V D  WLK +    +  R   +      L + ++ A AI   +    +G   G   S+ + S G  YGVP+ + +S PV +  +  W +V  + +++     ++    +++E+
Sbjct:    1 MAEPISVLVTGAAGQIAYSLLFSIAKGDVFGKDQPIILLLLDITPMLPVLEGVVMELQDCALPLLREVIATDKEEVAFKDLDAAILVGSMPRK--EGMERKDLLKANVAIFRSQGAALDKYAKKTVHVLVVGNPA----NTNCLIAAKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVPATHVKNVIIWGNHSSTQYPDVHHCKVSMSSG--------ELACFDAVKDDSWLKGDFIATVQQRGAAVIKARK-LSSAMSAAKAICDHMRDIWSGTPEGEFISMGVYSTGNPYGVPEDLIYSFPVQIKDKA-WKIVDSLAINDFSKSKMDATAAELMEE 323          
BLAST of Malate dehydrogenase 1B vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 105.916 bits (263), Expect = 1.556e-24
Identity = 83/339 (24.48%), Postives = 152/339 (44.84%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++ P +VV+ GA  +     + S+++GDV   +  + ++ +D    LP+L  + M   D A  L+R +  TD  E  F D    ++    +    +  E    +KAN   ++  G  ++KY    VKVL+V   A    N N +   +    IPK N   L+R    +  S VA +  V+   V N I+WGN     Y D ++++V+     V G E   +S    V +  WL+ +    +  R   +      L + ++ A AI   +   W    E  +I   V A+   YG+P+ + +S P+ +  +  W VV  + +++     ++    +++E+
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVG--SMPRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVN-----VSGSE---VSAYDAVKNDAWLRGDFISTVQLRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNK-TWKVVDGLPINDFSRAKMDATAAELVEE 323          
BLAST of Malate dehydrogenase 1B vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 105.916 bits (263), Expect = 2.084e-24
Identity = 84/345 (24.35%), Postives = 152/345 (44.06%), Query Frame = 2
Query:  401 IREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            +R     + P +VV+ GA  +     + S+++GDV   +  + ++ +D    LP+L  + M   D A  L+R +  TD  E  F D    ++         +  E    +KAN   ++  G  ++KY    VKVL+V   A    N N +   +    IPK N   L+R    +  S VA +  V+   V N I+WGN     Y D ++++V+     V G E   +S    V +  WL+ +    +  R   +      L + ++ A AI   +   W    E  +I   V A+   YG+P+ + +S P+ +  +  W VV  + +++     ++    +++E+
Sbjct:   12 VRAISPQAEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSMPRK--EGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVN-----VSGSE---VSAYDAVKNDAWLRGDFISTVQLRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNK-TWKVVDGLPINDFSRAKMDATAAELVEE 340          
BLAST of Malate dehydrogenase 1B vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 105.145 bits (261), Expect = 2.673e-24
Identity = 83/338 (24.56%), Postives = 150/338 (44.38%), Query Frame = 2
Query:  422 STPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQ-WINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            + P +VV+ GA  +     + S+++GDV   +  + ++ +D    LP+L  + M   D A  L+R +  TD  E  F D    ++         +  E    +KAN   ++  G  ++KY    VKVL+V   A    N N +   +    IPK N   L+R    +  S VA +  V+   V N I+WGN     Y D ++++V+     V G E   +S    V +  WL+ +    +  R   +      L + ++ A AI   +   W    E  +I   V A+   YG+P+ + +S P+ +  +  W VV  + +++     ++    +++E+
Sbjct:    4 AEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSM--PRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPA----NTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVN-----VSGSE---VSAYDAVKNDAWLRGDFISTVQLRGAAVIKARK-LSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNK-TWKVVDGLPINDFSRAKMDATAAELVEE 325          
BLAST of Malate dehydrogenase 1B vs. Planmine SMEST
Match: SMESG000070444.1 (SMESG000070444.1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.000e+0
Identity = 600/607 (98.85%), Postives = 600/607 (98.85%), Query Frame = 2
Query:   38 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEKTRIIYPWINNSNQSEMVIDESFIDSQINALLHEIYPKNEIPSILALITNXXXXXXXXXXXXXXXXXXXTTVSDLRKPPPVKERTDITNPESLPDAQNLSPTNNINAFXXXXXXXXXXXXXXXDPPEHLTTDPSTNDHEDQLE 1858
            MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDI EKTRIIYPWINNSNQSEMVIDESFIDSQINALLHEIYPKNEIPSILALITNSSLLLEDKLDISKSKDLLLTTVSDLRKPPPVKERTDITNPESLPDAQNLSPTNNINAF      ITETSITESDPPEHLTTDPSTNDHEDQLE
Sbjct:    1 MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSELCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFTSSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDINEKTRIIYPWINNSNQSEMVIDESFIDSQINALLHEIYPKNEIPSILALITNSSLLLEDKLDISKSKDLLLTTVSDLRKPPPVKERTDITNPESLPDAQNLSPTNNINAF-----EITETSITESDPPEHLTTDPSTNDHEDQLE 602          
BLAST of Malate dehydrogenase 1B vs. Planmine SMEST
Match: SMESG000056995.1 (SMESG000056995.1)

HSP 1 Score: 119.398 bits (298), Expect = 2.567e-29
Identity = 89/339 (26.25%), Postives = 155/339 (45.72%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADSSLVVIFDDRNPSP-ADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEIQFKSINECIQDIIEK 1432
            ++ P  VV+ GA  +     V S++ G+V   +  + +  +D    +P LK L M   D A+  + S+  TD+ + AF D    ++       P  +  E    +KAN   ++  G  ++KY     KV +V   A    N N + + +    IPK N   L+R    + ++ +A K  V   +V   ++WGN     Y D N+  VH +DG         +S+   V D  WLKN+    +  R   +      L + L+ A A+      W +G +     S+ + S+G YGVP  + FS PVT++  G W +VQ + + +   + ++   +++IE+
Sbjct:    1 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGPHQPINLHLLDIPVMVPSLKGLIMELEDCAFPTLHSVIATDDLKEAFTDVDFALMV---GAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTAKVCVVGNPA----NTNCLVMSKYAPSIPKENFSALTRLDHNRAQAQIAAKAGVPCDHVKRCVIWGNHSNTQYPDVNHGLVHGHDGK-------EVSIKDAVKDDTWLKNDFIKTVQTRGAAVIAARK-LSSALSAAKAVADHCRDWFHGTKEWV--SMAVYSDGSYGVPKDVIFSFPVTID-HGKWHIVQGLTIDDFSRQKLDLTGKELIEE 321          
BLAST of Malate dehydrogenase 1B vs. Planmine SMEST
Match: SMESG000027913.1 (SMESG000027913.1)

HSP 1 Score: 108.227 bits (269), Expect = 1.675e-25
Identity = 87/324 (26.85%), Postives = 145/324 (44.75%), Query Frame = 2
Query:  419 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADS--SLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSE 1384
            +S P  V+I GA  +     V +++ G V     ++ +   D  + +P LK L M   D ++  ++SI  T + +  F D   +L+V    R     D  E    +KAN   ++  G +++KY    VKV++V   A    N N   + +    IPK N   L+R    + +S +A K+ V  ++VH  ++WGN  G  Y D  N  V   +G         +SV + V +  WL+ E    +  R   +      L + ++ A AI      W  G       S+ + S+G Y  P  + FS PVT+   G WT+V  +++ +
Sbjct:    1 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRK----DGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPA----NTNCFILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGK-------EVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARK-LSSAMSAAKAICDHCRDWFKGTSDWV--SMAVYSDGSYNSPKDIIFSFPVTIK-NGKWTIVTGLKIDD 305          
The following BLAST results are available for this feature:
BLAST of Malate dehydrogenase 1B vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MDH1B3.655e-8135.91malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HG... [more]
MDH1B4.879e-8135.91malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HG... [more]
MDH1B1.915e-4630.52malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HG... [more]
MDH1B2.832e-2747.37malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HG... [more]
MDH1B1.287e-2647.79malate dehydrogenase 1B [Source:HGNC Symbol;Acc:HG... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mdh-18.481e-1523.17Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
mdh-11.934e-724.72Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
mdh-11.264e-622.75Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mdh11.424e-1523.91gene:FBgn0262782 transcript:FBtr0345435[more]
Mdh11.424e-1523.91gene:FBgn0262782 transcript:FBtr0080050[more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh1b5.512e-7632.83malate dehydrogenase 1B, NAD (soluble) [Source:NCB... [more]
mdh1aa2.271e-2626.67malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
mdh1ab3.270e-2625.15malate dehydrogenase 1Ab, NAD (soluble) [Source:ZF... [more]
mdh1aa6.256e-2626.55malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
mdh1aa3.820e-830.23malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MDH1B6.831e-7435.48malate dehydrogenase 1B [Source:NCBI gene;Acc:1004... [more]
mdh11.911e-2323.71malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh13.050e-2323.48malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh13.278e-2323.48malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh15.673e-2324.26malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mdh1b1.729e-7332.90malate dehydrogenase 1B, NAD (soluble) [Source:MGI... [more]
Mdh11.739e-2725.00malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh11.951e-2725.00malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh11.951e-2725.00malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh15.867e-927.04malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
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BLAST of Malate dehydrogenase 1B vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8T773|MDH1B_BRAFL7.725e-10937.53Putative malate dehydrogenase 1B OS=Branchiostoma ... [more]
sp|Q5I0G3|MDH1B_HUMAN2.343e-8035.91Putative malate dehydrogenase 1B OS=Homo sapiens O... [more]
sp|Q08BZ4|MDH1B_DANRE7.736e-7733.05Putative malate dehydrogenase 1B OS=Danio rerio OX... [more]
sp|A3KMX7|MDH1B_BOVIN5.663e-7533.88Putative malate dehydrogenase 1B OS=Bos taurus OX=... [more]
sp|Q5F204|MDH1B_MOUSE1.209e-7232.90Putative malate dehydrogenase 1B OS=Mus musculus O... [more]
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BLAST of Malate dehydrogenase 1B vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1QKI34.580e-11842.15Putative malate dehydrogenase 1B OS=Crassostrea gi... [more]
A0A1S3J5492.134e-11639.23putative malate dehydrogenase 1B OS=Lingula unguis... [more]
A0A1S3H2A83.766e-11639.23putative malate dehydrogenase 1B OS=Lingula unguis... [more]
A0A2C9JX511.676e-11341.31Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A210Q1L41.056e-11241.31Malate dehydrogenase 1B OS=Mizuhopecten yessoensis... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh1b1.384e-7431.48malate dehydrogenase 1B [Source:NCBI gene;Acc:1030... [more]
mdh1b2.313e-6730.82malate dehydrogenase 1B [Source:NCBI gene;Acc:1030... [more]
mdh1aa1.404e-2525.51malate dehydrogenase, cytoplasmic [Source:NCBI gen... [more]
mdh1ab2.614e-2525.37malate dehydrogenase, cytoplasmic [Source:NCBI gen... [more]
mdh1aa3.247e-2525.37malate dehydrogenase, cytoplasmic [Source:NCBI gen... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mdh1b5.208e-7833.41malate dehydrogenase 1B, NAD (soluble) [Source:NCB... [more]
ENSPMAT00000007050.12.781e-826.67pep scaffold:Pmarinus_7.0:GL479360:144570:153551:-... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Malate dehydrogenase 1B vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO458402.453e-8435.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412183.071e-2525.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Malate dehydrogenase 1B vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh1b4.712e-5729.40malate dehydrogenase 1B [Source:NCBI gene;Acc:1011... [more]
mdhb2.698e-2524.78cytosolic malate dehydrogenase B [Source:NCBI gene... [more]
mdh11.556e-2424.48malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
mdh12.084e-2424.35malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
mdh12.673e-2424.56malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
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BLAST of Malate dehydrogenase 1B vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000070444.10.000e+098.85SMESG000070444.1[more]
SMESG000056995.12.567e-2926.25SMESG000056995.1[more]
SMESG000027913.11.675e-2526.85SMESG000027913.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008051 ID=SMED30008051|Name=Malate dehydrogenase 1B|organism=Schmidtea mediterranea sexual|type=transcript|length=1950bp
ATTATCTAACTTAGTAACTATAATATCATTTATTTAAATGGCCAAAATTG
TAGTAGCAGGTTGCTCTGATTGTCCATATTTTGCCAAAGTAGAATTATTA
GCCGACAATTTAAAAATAAATGTTCCGAATTTTCACGTGCATAAAATAGT
GAAAACAAGAAAAGAATGGAGTGCTTGGTCAAGTGAATTGTGCAAAACAA
ACAATTGGAGTATAAAAGATTCACCAATAATATGGCGGGAACTTGTTGAT
CGAGGCGGCAAAGGACACTTGATTGGTGGTGCCAATGAATTTCAAGAGTA
TATCAAATGCTACTATAATGTCATATCAGATTTTACGTCATCTCATATGC
AAGATATTGCTTTAGATAATGTTCGTTTTAAAGAAAATGAAGATAAAGAA
ATTCGAGAATTCAAAGCACTTTCAACGCCGACTAAAGTCGTAATTGTAGG
AGCTGTGAATGAAGCCGTTCCTCATTTCGTTCTCTCTCTGTCTCGCGGTG
ACGTCCTCAACTCGAATACGGAACTGGCGATTGTATTTGTCGATAAAACC
GAACACCTGCCGATTCTGAAGAGCCTGCAAATGGGAACATTCGATCTGGC
TTATCGATTGGTGAGATCAATCTCGATCACCGATAATTTCGAGGCAGCCT
TTGCCGACAGCTCATTGGTCGTGATATTCGACGATCGAAACCCGTCGCCA
GCAGATCCCGACGAGTACATTCCGTGGATGAAAGCCAACCAGGAGTATTA
TGAATATATCGGGAATTTCATCGAGAAATATTGCTCGCTTGACGTGAAAG
TGTTACTCGTGCCGATAAAAGCCATTAGCTATCTTAATATCAATTTGATT
TCCATTCATCGAATGCTACAAAGGATTCCGAAATTCAATGTTGTCGGGTT
GTCGAGAACTGTCGAACAGCAAGTGCGCTCAGTTGTCGCTAGGAAACTCA
ACGTCAATCCATCGAATGTCCACAACAATATCGTGTGGGGCAATATTGGC
GGCAAGATGTACATCGATACCAACAACAGTCGGGTACATCGGTACGACGG
CGCGGTCATGGGACCGGAACCATTCTCACTGTCCGTAACGAAAACAGTTT
GGGATAAAAAGTGGCTGAAAAACGAAATGGAACCGTTGATTTTAAATCGG
TTCCAAGAGATTTCTGATTTAAACAACGGCCTAGGAACTCCATTGGCTCT
TGCGTCAGCTATTACTACTTTCCTCGGCCAATGGATTAATGGAGATGAGC
ATGGCTACATTCATTCACTGGTCATTGCATCGGAAGGTTGGTACGGAGTA
CCGGATGGAATGGCTTTCTCAATGCCTGTCACGCTTAATCCTCAAGGTTA
TTGGACGGTGGTTCAAGATGTTGAATTGTCAGAAATACAATTCAAAAGTA
TAAATGAATGCATCCAGGACATTATTGAAAAAACCCGAATCATTTATCCG
TGGATAAATAACAGCAATCAATCAGAAATGGTCATTGATGAATCCTTTAT
CGACAGTCAAATTAATGCCTTACTTCACGAGATATACCCCAAAAATGAAA
TTCCTTCAATACTAGCTTTAATTACTAATAGTTCCCTATTACTTGAAGAC
AAATTGGATATCAGTAAGAGTAAAGATTTGTTATTAACAACAGTATCCGA
CCTTCGGAAACCGCCACCAGTTAAAGAGAGAACCGATATTACAAACCCAG
AATCGCTTCCAGATGCGCAAAACCTTAGTCCTACTAATAATATTAATGCC
TTTGAAATTACCGAAACCTCAATTACCGAAACCTCAATTACCGAAAGTGA
TCCACCAGAACATTTAACAACTGATCCATCAACAAATGATCATGAAGATC
AATTAGAAAACAAAAATTTATAAAATTTCAATTTTTTTTATCATTTTTTT
TAAAGATTCACAAATTTATTATTTGACAAAAGTGTATTCTTTTGAACGAG
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protein sequence of SMED30008051-orf-1

>SMED30008051-orf-1 ID=SMED30008051-orf-1|Name=SMED30008051-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=612bp
MAKIVVAGCSDCPYFAKVELLADNLKINVPNFHVHKIVKTRKEWSAWSSE
LCKTNNWSIKDSPIIWRELVDRGGKGHLIGGANEFQEYIKCYYNVISDFT
SSHMQDIALDNVRFKENEDKEIREFKALSTPTKVVIVGAVNEAVPHFVLS
LSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDN
FEAAFADSSLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCS
LDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVV
ARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSV
TKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWI
NGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSEI
QFKSINECIQDIIEKTRIIYPWINNSNQSEMVIDESFIDSQINALLHEIY
PKNEIPSILALITNSSLLLEDKLDISKSKDLLLTTVSDLRKPPPVKERTD
ITNPESLPDAQNLSPTNNINAFEITETSITETSITESDPPEHLTTDPSTN
DHEDQLENKNL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000096ventral epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR010945Malate_DH_type2
IPR036291NAD(P)-bd_dom_sf
IPR022383Lactate/malate_DH_C
IPR015955Lactate_DH/Glyco_Ohase_4_C
IPR016040NAD(P)-bd_dom
Vocabulary: molecular function
TermDefinition
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0003824catalytic activity
GO:0016491oxidoreductase activity
GO:0016615malate dehydrogenase activity
GO:0030060L-malate dehydrogenase activity
GO:0051287NAD binding
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
GO:0005975carbohydrate metabolic process
GO:0006099tricarboxylic acid cycle
GO:0006107oxaloacetate metabolic process
GO:0006108malate metabolic process
GO:0006734NADH metabolic process
GO:0019752carboxylic acid metabolic process
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005739mitochondrion
GO:0005829cytosol
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022383Lactate/malate dehydrogenase, C-terminalPFAMPF02866Ldh_1_Ccoord: 297..465
e-value: 1.5E-11
score: 44.5
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalGENE3DG3DSA:3.90.110.10coord: 288..475
e-value: 3.8E-39
score: 136.1
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalSUPERFAMILYSSF56327LDH C-terminal domain-likecoord: 297..473
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 130..286
e-value: 2.3E-20
score: 74.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..606
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 590..606
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 554..589
NoneNo IPR availablePANTHERPTHR23382:SF1MALATE DEHYDROGENASE 1B-RELATEDcoord: 22..477
IPR010945Malate dehydrogenase, type 2PANTHERPTHR23382MALATE DEHYDROGENASEcoord: 22..477
IPR036291NAD(P)-binding domain superfamilySUPERFAMILYSSF51735NAD(P)-binding Rossmann-fold domainscoord: 130..258