Usp domain-containing protein

Overview
NameUsp domain-containing protein
Smed IDSMED30008017
Length (bp)678
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Usp domain-containing protein (SMED30008017) t-SNE clustered cells

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30008017) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Usp domain-containing protein (SMED30008017) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30008017) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30008017

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30008017h1SMcG0015162 Contig30716newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008017h1SMcG0015162 Contig30716uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008017h1SMcG0015163 Contig38434newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008017h1SMcG0015163 Contig38434uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008017h1SMcG0015163 Contig30716newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30008017h1SMcG0015163 Contig30716uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30008017h1SMcG0015163 dd_Smed_v4_1068_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30008017h1SMcG0015163 dd_Smed_v6_1068_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30008017h1SMcG0015163 dd_Smed_v4_1068_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|A0QZA1|Y3950_MYCS2 (Universal stress protein MSMEG_3950/MSMEI_3859 OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_3950 PE=1 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 5.167e-6
Identity = 38/151 (25.17%), Postives = 73/151 (48.34%), Query Frame = 3 
Query:  129 TERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIV 581
            TE  I++ +D+S  ++  V W           + +++++ P+  S+ AG  M   +    E+A    +  I++ +++V   + E    G   +  IR +S V   ++       A +V++ NRG G + R  LGS S  +LH+   PV++V
Sbjct:    6 TEYGILVGVDSSAESDAAVRWAAREASLHDAPITLMHVIAPVVVSWPAGPYMATVLECQEENA----RHAIEQAQKVVADCLGE--THGLTVQTEIRKES-VARTLIDASK--SAQMVVVGNRGMGALGRVLLGSTSTSLLHYASGPVVVV 147
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A183WUS6 (Usp domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS15878 PE=4 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 1.409e-44
Identity = 73/155 (47.10%), Postives = 105/155 (67.74%), Query Frame = 3 
Query:  123 DETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            +ET RV+++PID SE+ +R   WY+EN+ +P+D+V  +NIVEP+Y + + GL ME   LT   D    ++  ID GK L K+YI E +  G   + ++ VD+  G A++K   +H A+++++ NRG G IRRTFLGSVS+YVLHH   PVIIVPP
Sbjct:    5 NETPRVVLMPIDGSEHAQRAFQWYIENMKRPNDIVKFINIVEPVYATPAVGLTMETPPLT---DITKMMQDSIDNGKVLGKKYITEAKQCGVNCQAFLHVDTRPGAALLKSIEDHNANVIIMGNRGLGAIRRTFLGSVSEYVLHHAHIPVIIVPP 156
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A183VJ70 (Usp domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS54 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 2.562e-42
Identity = 70/157 (44.59%), Postives = 105/157 (66.88%), Query Frame = 3 
Query:  117 GDDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            GD  + RVI+IPID SE+ ER   WY++N+ +  D +  V+++EP+YN+ + GL ME+  +    D    +++ ID GK+L ++Y+HE +      + ++ VD+  G ++VK   +HKA ++L+ NRG G IRRTFLGSVSDY+LHH   PVIIVPP
Sbjct:    3 GDSGSGRVILIPIDGSEHCERAFRWYLDNMKRETDCIKFVHVIEPVYNTPAIGLTMESPPVP---DMTRVMEESIDAGKKLGQKYMHEAKAHKLTAQAFLHVDTKPGSSLVKAISDHKASVILMGNRGVGAIRRTFLGSVSDYILHHAHIPVIIVPP 156
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A4S2MD90 (Usp domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000850 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.030e-41
Identity = 71/157 (45.22%), Postives = 101/157 (64.33%), Query Frame = 3 
Query:  117 GDDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            G DE  R IV+P+D SE++ER   WYV N+ +P+D V  VNI+EP+Y S + G AME   L    D    + + ++ GK++ +  +H+ +      + ++ VDS  G AIVK   ++ AD+V++ NRG G +RRTFLGSVSDYVLHH   PV+IVPP
Sbjct:    5 GVDEKPRTIVLPLDGSEHSERAFRWYVNNVRRPNDNVKFVNIIEPVYTSPTFGAAMELPPL---PDISRVMTETVESGKKVCQEKMHQAKAYNINSQAFLYVDSRPGPAIVKAVQDYDADLVIMGNRGIGTVRRTFLGSVSDYVLHHSHAPVLIVPP 158
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A095AGD1 (Universal stress protein OS=Schistosoma haematobium OX=6185 GN=MS3_01105 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.046e-41
Identity = 71/152 (46.71%), Postives = 102/152 (67.11%), Query Frame = 3 
Query:  132 ERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            +RV+++PID SE+ ER   WY+ N+   +D+V  VNIVEP+YN+ + GL ME   LT   D    ++  IDKGK L K+YI E +      + ++ VD+  G AI+K   +H A+++++ +RG G++RRTFLGSVSDYVLHH   PV+IVPP
Sbjct:    8 QRVVLLPIDGSEHAERAFHWYLNNMKSSNDIVKFVNIVEPVYNTPAVGLTMETPPLT---DITKIMQDSIDKGKLLGKKYISEAKKYDINCQAFLHVDNRPGAAILKSIEDHNANLIIMGSRGLGVLRRTFLGSVSDYVLHHAHIPVVIVPP 156
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A430QIP7 (Usp domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0011032 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.046e-41
Identity = 71/152 (46.71%), Postives = 102/152 (67.11%), Query Frame = 3 
Query:  132 ERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            +RV+++PID SE+ ER   WY+ N+   +D+V  VNIVEP+YN+ + GL ME   LT   D    ++  IDKGK L K+YI E +      + ++ VD+  G AI+K   +H A+++++ +RG G++RRTFLGSVSDYVLHH   PV+IVPP
Sbjct:    8 QRVVLLPIDGSEHAERAFHWYLNNMKSSNDIVKFVNIVEPVYNTPAVGLTMETPPLT---DITKIMQDSIDKGKLLGKKYISEAKKYDINCQAFLHVDNRPGAAILKSIEDHNANLIIMGSRGLGVLRRTFLGSVSDYVLHHAHIPVVIVPP 156
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO33143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST34])

HSP 1 Score: 91.2781 bits (225), Expect = 3.069e-23
Identity = 50/154 (32.47%), Postives = 86/154 (55.84%), Query Frame = 3 
Query:  135 RVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVE----PIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVP 584
            R I+IP+D SE++ER  DWY E +  P D V +V+ +E    P+ + F         +    E+    +K+  ++ + +++ Y    ++K    +  + V    G+ I +V  +  A++++L  RG+G+IRRT LGSVSDYV+HH   PV ++P
Sbjct:    4 RKILIPVDGSEHSERAFDWYAELLHSPGDEVLVVHCIELPPVPLEHQFPF-------VFAYYEEWSAMVKETREQHEAMLRSYEDICKEKKLHYEIMMVVGKPAGDVICQVARDVSANLIVLGTRGQGMIRRTILGSVSDYVVHHSHLPVAVIP 150
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44805 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT2])

HSP 1 Score: 87.8113 bits (216), Expect = 6.304e-22
Identity = 49/151 (32.45%), Postives = 83/151 (54.97%), Query Frame = 3 
Query:  135 RVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            R IVIP+D S+++ER  DWY   + + +D V +V+     ++ ++A            ED E  +KK +D  K +++ Y  + +D   K    +    + GE I ++  +  AD +++ +RG G +RRT +GSVS++ LHH   P+ IVPP
Sbjct:   10 RKIVIPVDGSKHSERAFDWYKGALHRGNDEVFVVHA----FDPYAAPPTPYPYGFAFPEDWEQHMKKTVDDAKSVMEYYEKKCKDSKMKCT-MLTKPGDPGETICEIAKDKNADQIIMGSRGLGTVRRTIVGSVSEFCLHHTHIPMSIVPP 155
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO34658 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNQ5])

HSP 1 Score: 87.0409 bits (214), Expect = 1.465e-21
Identity = 48/161 (29.81%), Postives = 86/161 (53.42%), Query Frame = 3 
Query:  114 LGDDETERVIVIPIDNSEYTERTVDWYVENIMKPHD---MVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            +  +  +  +VI +D SE+++R  ++Y +N+ K  D   ++H  +I E        GLA         E  +  +++  ++ K+++ R+  + ++     K + +V  N GE I     E  AD V+L  RG+G +RRTF+GSVS+Y +HH   P+ +VPP
Sbjct:    1 MAQESQKSRVVIAVDGSEHSDRAFEFYSQNMHKKGDEVLLIHANDIAERHIQLHPYGLAT-------VEGWDKWLERCTEESKKMLSRFEKKCKENKFNCKLFTKV-GNPGEVICDFTEEKNADQVVLGCRGQGTVRRTFMGSVSEYCIHHATTPITVVPP 153
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUS8])

HSP 1 Score: 84.7297 bits (208), Expect = 8.860e-21
Identity = 48/153 (31.37%), Postives = 86/153 (56.21%), Query Frame = 3 
Query:  132 ERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVE-PIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            +R +VIP+D S+++ER  +WY +++ +P D V +++  E P   S  +  A    +L   ++    + + I   K+L++ Y  + +++G   +   +     GE I ++  +  A  V++ +RG G IRRT LGSVSDY +HH   PV ++PP
Sbjct:    6 KRRVVIPVDGSQHSERAFNWYRQHVHEPGDEVLIIHTQEQPTIPS--SPYAYGGTVL--PDEWNKAVDECIVNAKKLIEEYNKKCKEQGMTCR-LFKGSGQPGETICQLAKDLSAKHVVMGSRGCGTIRRTLLGSVSDYCVHHSSVPVTVIPP 153
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO49708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFF6])

HSP 1 Score: 84.3445 bits (207), Expect = 1.117e-20
Identity = 45/154 (29.22%), Postives = 91/154 (59.09%), Query Frame = 3 
Query:  132 ERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVE--PIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPP 587
            +R +++ ID+SE++ R  +WY ENI +  +++ +V+  E  PI+      +  +A   T+  +   + KK   + K+L++ +    +++  + + ++    N G AI+K+  + K + V++ +RG+ ++RRT +GSVSD+++HH   PV I PP
Sbjct:   13 KRRVLLAIDHSEHSMRAFEWYFENIHRDDNLLMLVHSQELPPIF------IPPDAFGTTLYNEWLAEAKKASLQSKKLLEGFERMCKERHCECEKHLLEGDNPGPAIIKLIKKSKPNYVVIGSRGQSMVRRTVMGSVSDFIIHHAHVPVCISPP 160
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020955.1 (SMESG000020955.1)

HSP 1 Score: 337.035 bits (863), Expect = 1.754e-119
Identity = 164/165 (99.39%), Postives = 165/165 (100.00%), Query Frame = 3 
Query:  102 MSTGLGDDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLHH 596
            MSTGLGDDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPI+NSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLHH
Sbjct:    1 MSTGLGDDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIFNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLHH 165
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020952.1 (SMESG000020952.1)

HSP 1 Score: 234.958 bits (598), Expect = 3.254e-79
Identity = 104/158 (65.82%), Postives = 136/158 (86.08%), Query Frame = 3 
Query:  120 DDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLH 593
            +DE ERVI++P+D SE+TER +DWYVEN+MKP+D+V M++IVEP+Y S + G+ ME A L + ED E+KIKKQ+DKGK LVK++ HEL++KGG+ KGY+ VDS VGEAI+KVCH HK D+++++NRGEG+IRRTFLGSVSDYVLHH DRPV+I+PP H
Sbjct:    6 NDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPV-EDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 162
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020882.1 (SMESG000020882.1)

HSP 1 Score: 223.787 bits (569), Expect = 1.083e-74
Identity = 102/156 (65.38%), Postives = 130/156 (83.33%), Query Frame = 3 
Query:  126 ETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLH 593
            E+ER+I+IPID SE + R +DWY  N+MK +D+VH+++IVEPIYN+ + G+ MEAA L M E+ + KI+KQIDKGK LV+ YI  L DKGGK KG+IRVDS VGE I+ VCH+H AD++L++NRGEG++RRTFLGSVSDYVLHH D+PVIIVPPLH
Sbjct:   16 ESERIIIIPIDKSEVSRRALDWYSNNMMKENDVVHLIHIVEPIYNTIAVGIGMEAAWLPM-EELDAKIQKQIDKGKALVREYISNLTDKGGKCKGFIRVDSRVGECIISVCHDHLADVILMANRGEGLLRRTFLGSVSDYVLHHADKPVIIVPPLH 170
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020884.1 (SMESG000020884.1)

HSP 1 Score: 186.037 bits (471), Expect = 2.068e-60
Identity = 86/130 (66.15%), Postives = 110/130 (84.62%), Query Frame = 3 
Query:  204 IMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLH 593
            +M+ +D+VH+++IVEPIYN+ + G+ MEAA L M E+ + KI+KQIDKGK LV+ YI  L DKGGK KG+IRVDS VGE I+ VCH+H AD++L++NRGEG++RRTFLGSVSDYVLHH D+PVIIVPPLH
Sbjct:    1 MMRENDVVHLIHIVEPIYNTIAVGIGMEAAWLPM-EELDAKIQKQIDKGKALVREYISNLTDKGGKCKGFIRVDSRVGECIISVCHDHLADVILMANRGEGLLRRTFLGSVSDYVLHHADKPVIIVPPLH 129
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020954.1 (SMESG000020954.1)

HSP 1 Score: 154.451 bits (389), Expect = 1.715e-47
Identity = 65/153 (42.48%), Postives = 109/153 (71.24%), Query Frame = 3 
Query:  132 ERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPL 590
            ER+I+IPIDN + ++R++ WY++N+MKP+D VH++ +++P YN  + GL  + ++ T   ++++K+ K+I+ GK+LV+ YI+EL  +G KG GYIR+   VG+ +V VC+EH  D ++++ R +   +    GS+S YV  H +RPVI+VPPL
Sbjct:    8 ERIIIIPIDNRDASKRSLAWYLDNMMKPNDTVHIIYVIKPKYNLSTVGLVYKISLATPIGNSDSKLHKRIEDGKELVRSYINELTTRGAKGTGYIRIHPKVGQTVVDVCNEHLGDYIIITKRKKSSFKENLFGSISHYVFSHSNRPVIMVPPL 160
The following BLAST results are available for this feature:
BLAST of Usp domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|A0QZA1|Y3950_MYCS25.167e-625.17Universal stress protein MSMEG_3950/MSMEI_3859 OS=... [more]
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BLAST of Usp domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183WUS61.409e-4447.10Usp domain-containing protein OS=Trichobilharzia r... [more]
A0A183VJ702.562e-4244.59Usp domain-containing protein OS=Trichobilharzia r... [more]
A0A4S2MD901.030e-4145.22Usp domain-containing protein OS=Opisthorchis feli... [more]
A0A095AGD11.046e-4146.71Universal stress protein OS=Schistosoma haematobiu... [more]
A0A430QIP71.046e-4146.71Usp domain-containing protein OS=Schistosoma bovis... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO331433.069e-2332.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448056.304e-2232.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO346581.465e-2129.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447468.860e-2131.37Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO497081.117e-2029.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020955.11.754e-11999.39SMESG000020955.1[more]
SMESG000020952.13.254e-7965.82SMESG000020952.1[more]
SMESG000020882.11.083e-7465.38SMESG000020882.1[more]
SMESG000020884.12.068e-6066.15SMESG000020884.1[more]
SMESG000020954.11.715e-4742.48SMESG000020954.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008017 ID=SMED30008017|Name=Usp domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=678bp
CTCAAACACTAATCAACGATTCTCAAGTGCTATGTGTCAGTAATAAAGGT
CAAAATATCTGAAATAATATAATTAAAATTGCAGATCTTAAAATCATTAA
AATGTCTACTGGTTTGGGTGATGACGAAACTGAGAGGGTAATAGTCATTC
CAATTGATAATAGTGAGTATACAGAAAGAACTGTAGATTGGTATGTAGAA
AATATAATGAAACCTCACGATATGGTTCACATGGTGAATATTGTTGAGCC
AATTTACAATTCATTCTCGGCTGGGTTGGCAATGGAAGCCGCAATTTTAA
CAATGAGTGAAGACGCTGAAACAAAAATCAAAAAACAAATCGACAAAGGA
AAGCAATTGGTAAAACGATATATCCATGAATTAAGAGACAAAGGAGGGAA
GGGTAAAGGTTATATTCGAGTTGATTCCAATGTTGGAGAAGCAATTGTTA
AAGTATGCCATGAGCATAAAGCAGATATTGTTTTGCTTTCAAATCGTGGC
GAAGGTATAATTCGACGAACCTTTCTGGGATCTGTTAGTGATTATGTTCT
TCATCATGTTGATAGACCAGTGATAATTGTCCCTCCTCTGCATCACTAAA
TGATAGCTGTTCATAATAAGATTAACTTTTCATTTTTGAATTTCATTAAA
ACTCATTTCGTTTTATAAAAAAAAAGAT
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protein sequence of SMED30008017-orf-1

>SMED30008017-orf-1 ID=SMED30008017-orf-1|Name=SMED30008017-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=166bp
MSTGLGDDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEP
IYNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGK
GKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVL
HHVDRPVIIVPPLHH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000240body wall musculature
PLANA:0000421tail
Vocabulary: INTERPRO
TermDefinition
IPR006015Universal_stress_UspA
IPR006016UspA
IPR014729Rossmann-like_a/b/a_fold
Vocabulary: biological process
TermDefinition
GO:0006950response to stress
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 11..29
score: 23.79
coord: 121..133
score: 27.69
coord: 139..161
score: 39.3
IPR006016UspAPFAMPF00582Uspcoord: 12..161
e-value: 9.9E-21
score: 74.7
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 8..165
e-value: 7.3E-29
score: 103.2
NoneNo IPR availablePANTHERPTHR46989FAMILY NOT NAMEDcoord: 12..162
NoneNo IPR availableCDDcd00293USP_Likecoord: 14..160
e-value: 3.31867E-19
score: 76.6398
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 14..162