Oxalate:formate antiporter

Overview
NameOxalate:formate antiporter
Smed IDSMED30008010
Length (bp)1599
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Oxalate:formate antiporter (SMED30008010) t-SNE clustered cells

Violin plots show distribution of expression levels for Oxalate:formate antiporter (SMED30008010) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Oxalate:formate antiporter (SMED30008010) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Oxalate:formate antiporter (SMED30008010) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Homology
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21)

HSP 1 Score: 72.4034 bits (176), Expect = 2.797e-13
Identity = 74/318 (23.27%), Postives = 138/318 (43.40%), Query Frame = 2
Query:  146 IFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTST------WIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIF-------------------YFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESI------------ERVSYDNLEFKP-HXXXXXXXXXXXXXXXXXNAIC----INLITCLYNEYYHELFGAQQLVTISTSGAAF-NCLGRIFWGFIIDKKPFKV 970
            +FG   +H+++G +++ G+++P++  +M+N    S       WI  +       AM +GG         ++  IG +++++++ L    +     +  + YGL  G G+G+ Y  A+   +    ++IG+I G+VA+GFG+S+ IF                   YFLQ          +P  F  L  IY ++Q +  I++ +   +   + ESI            ER  +DN E         E+     +  LF +  C     N+   LY  Y          + ++ S A+  N + RI WG++ D+  F++
Sbjct:   28 LFGAVSIHLTIGTYHTFGNMLPYMASYMRNFTDPSVRIEHMMWI-PTFQGCFPFAMVIGGLTSSTFSPRVSAFIGCFLVTSSVALSAYAIQHSFALFFIVYGLLFGLGSGIAYVTAVSTAINWAPDKIGVISGIVAAGFGLSSSIFAPIQTMIVNPLNLPATKDGYFLQP----ELLHRVPALFTKLAVIYGIMQAMALIVVCDPPFRKSRSTESILEEDDEDMDEDYERPVFDNDEDTTIQMTSSEMLKSPTFYCLFASLFCCSFYANMFYNLYKTYGESFIEDDMFMAMAFSIASVANAIARIGWGYLTDRTSFQI 340          
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A0H3YF10 (Slc16a-10 OS=Schmidtea mediterranea OX=79327 GN=slc16a-10 PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 2.991e-37
Identity = 109/303 (35.97%), Postives = 170/303 (56.11%), Query Frame = 2
Query:  137 YLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTST---------WIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIF-----YFLQNLFTGSCC---KYLPQSFLILGCIYAVIQLIGGILIK------EKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEY--YHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            Y+ +FGGFL+HIS+G  ++ G + P+I ++M N N+T+          WI G    G GA+MSLGG++E ++G+S T++ GG+IMS    L   T++  +  V ++YGL  G GTG+ YGVA+ +P +      GL+ G++ SGFG+ ++ F     YF+ +  T S       +P SF+ +GCIY +IQLI   LIK      E  N+N L I          +E KPH+++  ++FWI+WI   LN+    L   L   +  Y E+   + L+   T G  F+  GRI WG++ D+  +K+
Sbjct:   13 YVAVFGGFLVHISLGGIFTYGSMFPYILQYM-NLNQTNVRVVHPTDVGWIIGMATFGRGASMSLGGFIEHRLGASFTIVTGGFIMSFGYILTYFTISLHIIPVAVSYGLIVGIGTGIAYGVAMTIPTQWFPKHPGLLSGIILSGFGIGSVGFDLIHSYFIDDDITNSTLVNPSRVPVSFIAMGCIYFMIQLIAAPLIKRPETVEESVNQNNLNIFG----ETTKIELKPHQLIFYVNFWIIWITYALNSAFSLLFILLQKMWGKYLEISDLRLLIATCT-GGIFSIFGRIIWGYLTDRFGYKI 309          

HSP 2 Score: 55.0694 bits (131), Expect = 2.991e-37
Identity = 32/99 (32.32%), Postives = 56/99 (56.57%), Query Frame = 3
Query: 1020 VIMTVGTSILMALFATISLMNLTGKM------NEIVFGIIFCAINFFQ-SGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKD 1295
            +++   T +   +F+T  + +  GK+      N     +I+  I FF  SGNFSIFP ICA+++G+++F  N+G+L+T  S    I  II + +  ++D
Sbjct:  309 IVIWTSTLLFAIMFSTFYICSEVGKLSTPNGNNRFAMYLIWVIIMFFLISGNFSIFPAICAKVFGIQHFTGNFGVLYTGYSFTAVITPIIVLRVFADQD 407          
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8H205 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 6.343e-30
Identity = 90/335 (26.87%), Postives = 156/335 (46.57%), Query Frame = 2
Query:  125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIESIERVSYDNLEF------KPHXXXXXXXXXXXXXXXXXNA--------ICINLITCLYNEYYHELFGAQQ------LVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            + WK +  + GGFL+H ++G  Y+ G++ P++  +M+  + +       +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS  + L    +   L ++++TYGL  G GTG+ Y VA+   +    +R GL+ G+V +GFG  +L+F   Q L+            G   +Y         +P  F+ LG IYA++Q +G  L++     E   + EL  E+ +  +            KP               +              +N  + +Y    ++ FG  +      L  + +  AAFN   R+ WG + D+  ++ 
Sbjct:    3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPRAWKPRRRMDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 337          

HSP 2 Score: 49.6766 bits (117), Expect = 6.343e-30
Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3
Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367
            M +++ G ++LM+ F       L+G      + +  CA+    +GNF++FP  CA+LYG      NYG+LFTA + +  I  +++  +   KD + +  +F+ GAA S L  +  L++ 
Sbjct:  339 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 446          
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 7.463e-29
Identity = 95/341 (27.86%), Postives = 165/341 (48.39%), Query Frame = 2
Query:  125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIE---------------------SIERVSYDN----LEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEY-YHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            + WK +  + GGFL+H ++G  Y+ G++ P++  +M+  + +       +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS  + L    +   L ++++TYGL  G GTG+ Y VA+   +    +R GL+ G+V +GFG  +L+F   Q L+            G   +Y         +P  F+ LG IYA++Q +G  L++     E   + EL  E                     ++E   + N    L+  P +++ +  FW+LW    +N+  +  I   Y  +   +      L  + +  AAFN   R+ WG + D+  ++ 
Sbjct:    3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 343          

HSP 2 Score: 49.2914 bits (116), Expect = 7.463e-29
Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3
Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367
            M +++ G ++LM+ F       L+G      + +  CA+    +GNF++FP  CA+LYG      NYG+LFTA + +  I  +++  +   KD + +  +F+ GAA S L  +  L++ 
Sbjct:  345 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 452          
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A267H5U2 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g2 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 7.713e-29
Identity = 95/341 (27.86%), Postives = 165/341 (48.39%), Query Frame = 2
Query:  125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIE---------------------SIERVSYDN----LEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEY-YHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            + WK +  + GGFL+H ++G  Y+ G++ P++  +M+  + +       +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS  + L    +   L ++++TYGL  G GTG+ Y VA+   +    +R GL+ G+V +GFG  +L+F   Q L+            G   +Y         +P  F+ LG IYA++Q +G  L++     E   + EL  E                     ++E   + N    L+  P +++ +  FW+LW    +N+  +  I   Y  +   +      L  + +  AAFN   R+ WG + D+  ++ 
Sbjct:    5 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 345          

HSP 2 Score: 49.2914 bits (116), Expect = 7.713e-29
Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3
Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367
            M +++ G ++LM+ F       L+G      + +  CA+    +GNF++FP  CA+LYG      NYG+LFTA + +  I  +++  +   KD + +  +F+ GAA S L  +  L++ 
Sbjct:  347 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 454          
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8HYG0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 8.308e-29
Identity = 90/341 (26.39%), Postives = 159/341 (46.63%), Query Frame = 2
Query:  125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIESIERVSYDNL---EFKPHXXXXXXXXXXXXXXXXXNA-----------------ICINLITCLYNEYYHELFGAQQ------LVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            + WK +  + GGFL+H ++G  Y+ G++ P++  +M+  + +       +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS  + L    +   L ++++TYGL  G GTG+ Y VA+   +    +R GL+ G+V +GFG  +L+F   Q L+            G   +Y         +P  F+ LG IYA++Q +G  L++     E   + EL  E+ +  +       E  P + +              ++                   +N  + +Y    ++ FG  +      L  + +  AAFN   R+ WG + D+  ++ 
Sbjct:    3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGESDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 343          

HSP 2 Score: 49.2914 bits (116), Expect = 8.308e-29
Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3
Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367
            M +++ G ++LM+ F       L+G      + +  CA+    +GNF++FP  CA+LYG      NYG+LFTA + +  I  +++  +   KD + +  +F+ GAA S L  +  L++ 
Sbjct:  345 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 452          
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1)

HSP 1 Score: 461.455 bits (1186), Expect = 0.000e+0
Identity = 262/288 (90.97%), Postives = 273/288 (94.79%), Query Frame = 2
Query:  107 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            M F F+S+ KYLPI G FLLH+SVG F+S G I P I+++M N NKTSTWI GSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+
Sbjct:    1 MRFKFTSYGKYLPILGSFLLHLSVGCFFSIGQIKPQIDEYMGNNNKTSTWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 288          

HSP 2 Score: 195.282 bits (495), Expect = 0.000e+0
Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3
Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406
            F ITMVIMTVGTSILMALFATISLMNLTGKMN  VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII    S +K+ +K +F  G ALS LS ++ +VYRGKSLI+RTN LSI
Sbjct:  286 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 419          
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069673.1 (SMESG000069673.1)

HSP 1 Score: 418.313 bits (1074), Expect = 0.000e+0
Identity = 242/297 (81.48%), Postives = 260/297 (87.54%), Query Frame = 2
Query:  107 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERV---------SYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINK+MKNENKTSTWIFGSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPL+IVK R+GL +GVV SGFGVSA IF +LQN+ +      LP SFLI+GCIYAVIQLIG IL+K+KAN+N+L I+S E           S   +EFKPHEILLELDFWILWI  FLNAI +N +T LYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+
Sbjct:    1 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKYMKNENKTSTWIFGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLKIVKKRVGLFLGVVVSGFGVSATIFDYLQNVLSSDYTN-LPLSFLIMGCIYAVIQLIGAILLKKKANENDLTIQSNETSFNYFEIFLNSKSKMEFKPHEILLELDFWILWITFFLNAIGVNFLTTLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 296          

HSP 2 Score: 250.366 bits (638), Expect = 0.000e+0
Identity = 128/134 (95.52%), Postives = 130/134 (97.01%), Query Frame = 3
Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406
            F ITMVIMTVGTSILMALF+TISLMNLTGKMNEIVFGIIFCAINFFQSGNF IFPF+CAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLM LVYRGKSLIIRTNAL I
Sbjct:  294 FKITMVIMTVGTSILMALFSTISLMNLTGKMNEIVFGIIFCAINFFQSGNFCIFPFMCAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMTLVYRGKSLIIRTNALLI 427          
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1)

HSP 1 Score: 453.366 bits (1165), Expect = 3.791e-179
Identity = 262/297 (88.22%), Postives = 273/297 (91.92%), Query Frame = 2
Query:  107 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIER---------VSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            M F F+S+ KYLPI G FLLH+SVG F+S G I P I+++M N NKTSTWI GSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIER         VSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+
Sbjct:    1 MRFKFTSYGKYLPILGSFLLHLSVGCFFSIGQIKPQIDEYMGNNNKTSTWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERSFNYFGNNLVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 297          

HSP 2 Score: 195.282 bits (495), Expect = 3.791e-179
Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3
Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406
            F ITMVIMTVGTSILMALFATISLMNLTGKMN  VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII    S +K+ +K +F  G ALS LS ++ +VYRGKSLI+RTN LSI
Sbjct:  295 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 428          
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1)

HSP 1 Score: 414.461 bits (1064), Expect = 1.601e-167
Identity = 239/248 (96.37%), Postives = 243/248 (97.98%), Query Frame = 2
Query:  227 MKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            M N NKTSTWI GSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+
Sbjct:    1 MGNNNKTSTWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 248          

HSP 2 Score: 195.667 bits (496), Expect = 1.601e-167
Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3
Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406
            F ITMVIMTVGTSILMALFATISLMNLTGKMN  VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII    S +K+ +K +F  G ALS LS ++ +VYRGKSLI+RTN LSI
Sbjct:  246 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 379          
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1)

HSP 1 Score: 395.201 bits (1014), Expect = 1.102e-161
Identity = 215/221 (97.29%), Postives = 219/221 (99.10%), Query Frame = 2
Query:  308 YMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970
            Y+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+
Sbjct:   10 YVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 230          

HSP 2 Score: 195.282 bits (495), Expect = 1.102e-161
Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3
Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406
            F ITMVIMTVGTSILMALFATISLMNLTGKMN  VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII    S +K+ +K +F  G ALS LS ++ +VYRGKSLI+RTN LSI
Sbjct:  228 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 361          
The following BLAST results are available for this feature:
BLAST of Oxalate:formate antiporter vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Y59A8B.212.797e-1323.27pep chromosome:WBcel235:V:17986260:17994546:1 gene... [more]
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YF102.991e-3735.97Slc16a-10 OS=Schmidtea mediterranea OX=79327 GN=sl... [more]
A0A1I8H2056.343e-3026.87MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8HL507.463e-2927.86MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267H5U27.713e-2927.86MFS domain-containing protein (Fragment) OS=Macros... [more]
A0A1I8HYG08.308e-2926.39MFS domain-containing protein OS=Macrostomum ligna... [more]
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069670.10.000e+090.97SMESG000069670.1[more]
SMESG000069673.10.000e+081.48SMESG000069673.1[more]
SMESG000069670.13.791e-17988.22SMESG000069670.1[more]
SMESG000069670.11.601e-16796.37SMESG000069670.1[more]
SMESG000069670.11.102e-16197.29SMESG000069670.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008010 ID=SMED30008010|Name=Oxalate:formate antiporter|organism=Schmidtea mediterranea sexual|type=transcript|length=1599bp
TTATCATTCAAATTATTAAAATAATTCTGAATGTTAATTAAATTTTATTT
AATAAATTTCTTTTAATAATAAAATTTATATAAATCATAAAAAGCAATCA
AATGAAATGAGTTTCGTGTTTTCTTCACATTGGAAATATTTGCCAATTTT
TGGAGGATTTCTATTGCATATATCGGTCGGATATTTTTATTCAGCTGGCC
ACATAATGCCTCATATTAACAAATTTATGAAAAATGAGAATAAAACGTCA
ACATGGATTTTTGGTTCCACATTACTAGGAATGGGAGCAGCAATGTCATT
GGGTGGATACATGGAAATGAAAATCGGTTCATCGCTGACAGTTTTAATTG
GCGGGTGGATAATGAGTGCCAATATCTGCCTGATGTGTGTGACCATGACA
TGTGGACTCACAGTTGTTATCCTTACCTATGGACTTTTTGCTGGATTTGG
AACTGGACTTGTGTATGGAGTTGCATTAGGTGTGCCATTAAGGATTGTGA
AAAATCGTATCGGTCTCATTATCGGAGTGGTCGCTTCAGGTTTTGGAGTT
TCGGCTCTTATTTTTTATTTCCTGCAAAATCTTTTCACTGGATCCTGTTG
CAAATATCTACCGCAATCATTTCTTATTTTGGGATGTATTTATGCAGTTA
TTCAATTAATTGGAGGAATCCTAATAAAAGAAAAGGCAAATAAAAATGAA
TTGGCAATTGAATCTATAGAAAGGGTTTCATATGATAATTTGGAATTCAA
ACCACATGAAATTCTGTTGGAGTTAGATTTCTGGATTCTTTGGATTATAC
TTTTCCTAAATGCGATTTGTATAAATCTTATAACATGTCTATACAATGAA
TATTATCATGAACTATTTGGTGCCCAACAGTTGGTAACGATATCCACCTC
TGGAGCAGCCTTTAATTGTTTAGGAAGAATATTCTGGGGTTTTATAATCG
ACAAAAAGCCTTTTAAAGTATTTTAAGTAAACAATTTCGATTTGCCCTTG
ACATTTCTAGATAACAATGGTCATAATGACAGTGGGTACCTCCATTCTCA
TGGCATTATTTGCAACTATTTCTTTGATGAATTTGACTGGAAAAATGAAT
GAAATTGTTTTTGGAATCATCTTTTGTGCAATCAACTTTTTCCAATCTGG
GAACTTCTCTATTTTCCCCTTTATCTGCGCTCAGTTATACGGAGTAAAAT
ATTTTGTTTGCAATTATGGAATGCTGTTTACAGCTTGTTCTGTTGCTTGC
ACCATCGTCAATATCATTTCAATGAATATTTCCAGAGAAAAAGATAATTT
GAAGTTTGTATTCGTGGCTGGTGCTGCCTTGTCAATTTTAAGTACCCTGA
TGATATTAGTTTACCGAGGAAAATCTTTAATCATTCGGACAAATGCACTT
TCAATATTATAAATGTCATTGTTACATTATAAAGATTCCTGAAATTATAT
ATTAAATATTGTTTGCATCTAATTTTTTCATAAGTTCGGAACATTCAAGA
AAGCATTCTGTTTCAGAATTTACATAATTTGTAGAAACCAAGAAATAAAT
TGTTTTGATCGTTTCTGATCTCCTTTGTTGATTTGTATTTTATTCCCTT
back to top

protein sequence of SMED30008010-orf-1

>SMED30008010-orf-1 ID=SMED30008010-orf-1|Name=SMED30008010-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=290bp
MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTW
IFGSTLLGMGAAMSLGGYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCG
LTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSA
LIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELA
IESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYY
HELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKVF*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 6..286
e-value: 3.5E-15
score: 57.4
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 10..284
NoneNo IPR availablePANTHERPTHR11360:SF3OXALATE/FORMATE ANTIPORTERcoord: 10..284
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 10..283
e-value: 1.56388E-34
score: 126.956
NoneNo IPR availableTMHMMTMhelixcoord: 73..95
NoneNo IPR availableTMHMMTMhelixcoord: 134..156
NoneNo IPR availableTMHMMTMhelixcoord: 102..124
NoneNo IPR availableTMHMMTMhelixcoord: 259..281
NoneNo IPR availableTMHMMTMhelixcoord: 50..69
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 168..190
NoneNo IPR availableTMHMMTMhelixcoord: 225..247
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 17..283
e-value: 1.2E-14
score: 54.1
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 9..284