Oxalate:formate antiporter
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Homology
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21) HSP 1 Score: 72.4034 bits (176), Expect = 2.797e-13 Identity = 74/318 (23.27%), Postives = 138/318 (43.40%), Query Frame = 2 Query: 146 IFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTST------WIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIF-------------------YFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESI------------ERVSYDNLEFKP-HXXXXXXXXXXXXXXXXXNAIC----INLITCLYNEYYHELFGAQQLVTISTSGAAF-NCLGRIFWGFIIDKKPFKV 970 +FG +H+++G +++ G+++P++ +M+N S WI + AM +GG ++ IG +++++++ L + + + YGL G G+G+ Y A+ + ++IG+I G+VA+GFG+S+ IF YFLQ +P F L IY ++Q + I++ + + + ESI ER +DN E E+ + LF + C N+ LY Y + ++ S A+ N + RI WG++ D+ F++ Sbjct: 28 LFGAVSIHLTIGTYHTFGNMLPYMASYMRNFTDPSVRIEHMMWI-PTFQGCFPFAMVIGGLTSSTFSPRVSAFIGCFLVTSSVALSAYAIQHSFALFFIVYGLLFGLGSGIAYVTAVSTAINWAPDKIGVISGIVAAGFGLSSSIFAPIQTMIVNPLNLPATKDGYFLQP----ELLHRVPALFTKLAVIYGIMQAMALIVVCDPPFRKSRSTESILEEDDEDMDEDYERPVFDNDEDTTIQMTSSEMLKSPTFYCLFASLFCCSFYANMFYNLYKTYGESFIEDDMFMAMAFSIASVANAIARIGWGYLTDRTSFQI 340
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A0H3YF10 (Slc16a-10 OS=Schmidtea mediterranea OX=79327 GN=slc16a-10 PE=2 SV=1) HSP 1 Score: 131.339 bits (329), Expect = 2.991e-37 Identity = 109/303 (35.97%), Postives = 170/303 (56.11%), Query Frame = 2 Query: 137 YLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTST---------WIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIF-----YFLQNLFTGSCC---KYLPQSFLILGCIYAVIQLIGGILIK------EKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEY--YHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 Y+ +FGGFL+HIS+G ++ G + P+I ++M N N+T+ WI G G GA+MSLGG++E ++G+S T++ GG+IMS L T++ + V ++YGL G GTG+ YGVA+ +P + GL+ G++ SGFG+ ++ F YF+ + T S +P SF+ +GCIY +IQLI LIK E N+N L I +E KPH+++ ++FWI+WI LN+ L L + Y E+ + L+ T G F+ GRI WG++ D+ +K+ Sbjct: 13 YVAVFGGFLVHISLGGIFTYGSMFPYILQYM-NLNQTNVRVVHPTDVGWIIGMATFGRGASMSLGGFIEHRLGASFTIVTGGFIMSFGYILTYFTISLHIIPVAVSYGLIVGIGTGIAYGVAMTIPTQWFPKHPGLLSGIILSGFGIGSVGFDLIHSYFIDDDITNSTLVNPSRVPVSFIAMGCIYFMIQLIAAPLIKRPETVEESVNQNNLNIFG----ETTKIELKPHQLIFYVNFWIIWITYALNSAFSLLFILLQKMWGKYLEISDLRLLIATCT-GGIFSIFGRIIWGYLTDRFGYKI 309 HSP 2 Score: 55.0694 bits (131), Expect = 2.991e-37 Identity = 32/99 (32.32%), Postives = 56/99 (56.57%), Query Frame = 3 Query: 1020 VIMTVGTSILMALFATISLMNLTGKM------NEIVFGIIFCAINFFQ-SGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKD 1295 +++ T + +F+T + + GK+ N +I+ I FF SGNFSIFP ICA+++G+++F N+G+L+T S I II + + ++D Sbjct: 309 IVIWTSTLLFAIMFSTFYICSEVGKLSTPNGNNRFAMYLIWVIIMFFLISGNFSIFPAICAKVFGIQHFTGNFGVLYTGYSFTAVITPIIVLRVFADQD 407
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8H205 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 112.079 bits (279), Expect = 6.343e-30 Identity = 90/335 (26.87%), Postives = 156/335 (46.57%), Query Frame = 2 Query: 125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIESIERVSYDNLEF------KPHXXXXXXXXXXXXXXXXXNA--------ICINLITCLYNEYYHELFGAQQ------LVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 + WK + + GGFL+H ++G Y+ G++ P++ +M+ + + +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS + L + L ++++TYGL G GTG+ Y VA+ + +R GL+ G+V +GFG +L+F Q L+ G +Y +P F+ LG IYA++Q +G L++ E + EL E+ + + KP + +N + +Y ++ FG + L + + AAFN R+ WG + D+ ++ Sbjct: 3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPRAWKPRRRMDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 337 HSP 2 Score: 49.6766 bits (117), Expect = 6.343e-30 Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3 Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367 M +++ G ++LM+ F L+G + + CA+ +GNF++FP CA+LYG NYG+LFTA + + I +++ + KD + + +F+ GAA S L + L++ Sbjct: 339 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 446
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 108.997 bits (271), Expect = 7.463e-29 Identity = 95/341 (27.86%), Postives = 165/341 (48.39%), Query Frame = 2 Query: 125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIE---------------------SIERVSYDN----LEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEY-YHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 + WK + + GGFL+H ++G Y+ G++ P++ +M+ + + +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS + L + L ++++TYGL G GTG+ Y VA+ + +R GL+ G+V +GFG +L+F Q L+ G +Y +P F+ LG IYA++Q +G L++ E + EL E ++E + N L+ P +++ + FW+LW +N+ + I Y + + L + + AAFN R+ WG + D+ ++ Sbjct: 3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 343 HSP 2 Score: 49.2914 bits (116), Expect = 7.463e-29 Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3 Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367 M +++ G ++LM+ F L+G + + CA+ +GNF++FP CA+LYG NYG+LFTA + + I +++ + KD + + +F+ GAA S L + L++ Sbjct: 345 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 452
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A267H5U2 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g2 PE=4 SV=1) HSP 1 Score: 108.612 bits (270), Expect = 7.713e-29 Identity = 95/341 (27.86%), Postives = 165/341 (48.39%), Query Frame = 2 Query: 125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIE---------------------SIERVSYDN----LEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEY-YHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 + WK + + GGFL+H ++G Y+ G++ P++ +M+ + + +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS + L + L ++++TYGL G GTG+ Y VA+ + +R GL+ G+V +GFG +L+F Q L+ G +Y +P F+ LG IYA++Q +G L++ E + EL E ++E + N L+ P +++ + FW+LW +N+ + I Y + + L + + AAFN R+ WG + D+ ++ Sbjct: 5 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 345 HSP 2 Score: 49.2914 bits (116), Expect = 7.713e-29 Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3 Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367 M +++ G ++LM+ F L+G + + CA+ +GNF++FP CA+LYG NYG+LFTA + + I +++ + KD + + +F+ GAA S L + L++ Sbjct: 347 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 454
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8HYG0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 108.612 bits (270), Expect = 8.308e-29 Identity = 90/341 (26.39%), Postives = 159/341 (46.63%), Query Frame = 2 Query: 125 SHWKYL-PIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKT-------STWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLF-----------TGSCCKY---------LPQSFLILGCIYAVIQLIGGILIK-----EKANKNELAIESIERVSYDNL---EFKPHXXXXXXXXXXXXXXXXXNA-----------------ICINLITCLYNEYYHELFGAQQ------LVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 + WK + + GGFL+H ++G Y+ G++ P++ +M+ + + +TWIFG ++ G GA+M+ GG +E +IG+ LT L+G WIMS + L + L ++++TYGL G GTG+ Y VA+ + +R GL+ G+V +GFG +L+F Q L+ G +Y +P F+ LG IYA++Q +G L++ E + EL E+ + + E P + + ++ +N + +Y ++ FG + L + + AAFN R+ WG + D+ ++ Sbjct: 3 TPWKGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQETALDEGSPSKPVAMETSEHGNRGGESDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRA 343 HSP 2 Score: 49.2914 bits (116), Expect = 8.308e-29 Identity = 36/119 (30.25%), Postives = 64/119 (53.78%), Query Frame = 3 Query: 1017 MVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKF--VFVAGAALSILSTLMILVYR 1367 M +++ G ++LM+ F L+G + + CA+ +GNF++FP CA+LYG NYG+LFTA + + I +++ + KD + + +F+ GAA S L + L++ Sbjct: 345 MFVLSFGMAVLMSTF------TLSGA--PWYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL---KDAIGWLGLFLLGAAFSTLGAVFTLLFE 452
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1) HSP 1 Score: 461.455 bits (1186), Expect = 0.000e+0 Identity = 262/288 (90.97%), Postives = 273/288 (94.79%), Query Frame = 2 Query: 107 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 M F F+S+ KYLPI G FLLH+SVG F+S G I P I+++M N NKTSTWI GSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+ Sbjct: 1 MRFKFTSYGKYLPILGSFLLHLSVGCFFSIGQIKPQIDEYMGNNNKTSTWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 288 HSP 2 Score: 195.282 bits (495), Expect = 0.000e+0 Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3 Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406 F ITMVIMTVGTSILMALFATISLMNLTGKMN VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII S +K+ +K +F G ALS LS ++ +VYRGKSLI+RTN LSI Sbjct: 286 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 419
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069673.1 (SMESG000069673.1) HSP 1 Score: 418.313 bits (1074), Expect = 0.000e+0 Identity = 242/297 (81.48%), Postives = 260/297 (87.54%), Query Frame = 2 Query: 107 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERV---------SYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINK+MKNENKTSTWIFGSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPL+IVK R+GL +GVV SGFGVSA IF +LQN+ + LP SFLI+GCIYAVIQLIG IL+K+KAN+N+L I+S E S +EFKPHEILLELDFWILWI FLNAI +N +T LYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+ Sbjct: 1 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKYMKNENKTSTWIFGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLKIVKKRVGLFLGVVVSGFGVSATIFDYLQNVLSSDYTN-LPLSFLIMGCIYAVIQLIGAILLKKKANENDLTIQSNETSFNYFEIFLNSKSKMEFKPHEILLELDFWILWITFFLNAIGVNFLTTLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 296 HSP 2 Score: 250.366 bits (638), Expect = 0.000e+0 Identity = 128/134 (95.52%), Postives = 130/134 (97.01%), Query Frame = 3 Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406 F ITMVIMTVGTSILMALF+TISLMNLTGKMNEIVFGIIFCAINFFQSGNF IFPF+CAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLM LVYRGKSLIIRTNAL I Sbjct: 294 FKITMVIMTVGTSILMALFSTISLMNLTGKMNEIVFGIIFCAINFFQSGNFCIFPFMCAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMTLVYRGKSLIIRTNALLI 427
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1) HSP 1 Score: 453.366 bits (1165), Expect = 3.791e-179 Identity = 262/297 (88.22%), Postives = 273/297 (91.92%), Query Frame = 2 Query: 107 MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIER---------VSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 M F F+S+ KYLPI G FLLH+SVG F+S G I P I+++M N NKTSTWI GSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIER VSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+ Sbjct: 1 MRFKFTSYGKYLPILGSFLLHLSVGCFFSIGQIKPQIDEYMGNNNKTSTWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERSFNYFGNNLVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 297 HSP 2 Score: 195.282 bits (495), Expect = 3.791e-179 Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3 Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406 F ITMVIMTVGTSILMALFATISLMNLTGKMN VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII S +K+ +K +F G ALS LS ++ +VYRGKSLI+RTN LSI Sbjct: 295 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 428
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1) HSP 1 Score: 414.461 bits (1064), Expect = 1.601e-167 Identity = 239/248 (96.37%), Postives = 243/248 (97.98%), Query Frame = 2 Query: 227 MKNENKTSTWIFGSTXXXXXXXXXXXXYMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 M N NKTSTWI GSTLLGMGAAMSLGGY+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+ Sbjct: 1 MGNNNKTSTWIVGSTLLGMGAAMSLGGYVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 248 HSP 2 Score: 195.667 bits (496), Expect = 1.601e-167 Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3 Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406 F ITMVIMTVGTSILMALFATISLMNLTGKMN VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII S +K+ +K +F G ALS LS ++ +VYRGKSLI+RTN LSI Sbjct: 246 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 379
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000069670.1 (SMESG000069670.1) HSP 1 Score: 395.201 bits (1014), Expect = 1.102e-161 Identity = 215/221 (97.29%), Postives = 219/221 (99.10%), Query Frame = 2 Query: 308 YMEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPLRIVKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHXXXXXXXXXXXXXXXXXNAICINLITCLYNEYYHELFGAQQLVTISTSGAAFNCLGRIFWGFIIDKKPFKV 970 Y+EMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVP+RI+KNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQ LVTIS SGAAFNCLGRIFWGFIIDKKPFK+ Sbjct: 10 YVEMKIGSSLTVLIGGWIMSANICLMCVTMTCGLTVVILTYGLFAGFGTGLVYGVALGVPIRIMKNRIGLIIGVVASGFGVSALIFYFLQNLFTGSCCKYLPQSFLILGCIYAVIQLIGGILIKEKANKNELAIESIERVSYDNLEFKPHEILLELDFWILWIILFLNAICINLITCLYNEYYHELFGAQHLVTISISGAAFNCLGRIFWGFIIDKKPFKI 230 HSP 2 Score: 195.282 bits (495), Expect = 1.102e-161 Identity = 103/134 (76.87%), Postives = 113/134 (84.33%), Query Frame = 3 Query: 1005 F*ITMVIMTVGTSILMALFATISLMNLTGKMNEIVFGIIFCAINFFQSGNFSIFPFICAQLYGVKYFVCNYGMLFTACSVACTIVNIISMNISREKDNLKFVFVAGAALSILSTLMILVYRGKSLIIRTNALSI 1406 F ITMVIMTVGTSILMALFATISLMNLTGKMN VFGIIFCAINFFQSGNF IFPF+CA+LYGVKYFVCNYGMLFTACS A T VNII S +K+ +K +F G ALS LS ++ +VYRGKSLI+RTN LSI Sbjct: 228 FKITMVIMTVGTSILMALFATISLMNLTGKMNGYVFGIIFCAINFFQSGNFCIFPFMCARLYGVKYFVCNYGMLFTACSAAGTTVNIILTTTSVDKEIIKTLFFTGTALSSLSAMVTIVYRGKSLIVRTNFLSI 361 The following BLAST results are available for this feature:
BLAST of Oxalate:formate antiporter vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of Oxalate:formate antiporter vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Oxalate:formate antiporter vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Oxalate:formate antiporter vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30008010 ID=SMED30008010|Name=Oxalate:formate antiporter|organism=Schmidtea mediterranea sexual|type=transcript|length=1599bpback to top protein sequence of SMED30008010-orf-1 >SMED30008010-orf-1 ID=SMED30008010-orf-1|Name=SMED30008010-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=290bp MSFVFSSHWKYLPIFGGFLLHISVGYFYSAGHIMPHINKFMKNENKTSTWback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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