Venom allergen 5

Overview
NameVenom allergen 5
Smed IDSMED30008009
Length (bp)667
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Venom allergen 5 (SMED30008009) t-SNE clustered cells

Violin plots show distribution of expression levels for Venom allergen 5 (SMED30008009) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30008009

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30008009h1SMcG0021319 SmedASXL_018784SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30008009h1SMcG0021319 SmedASXL_018784SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30008009h1SMcG0021319 OX_Smed_1.0.16988ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 112.849 bits (281), Expect = 1.510e-28
Identity = 63/185 (34.05%), Postives = 100/185 (54.05%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD---DVTEGIKMWYDEVKGYNFQSEKCSV-STCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T ++K+ +++ HN YR +V+       P A++ML ++W  E  A A+ +  +CV+ H+    R+       G+N+ +  +D   DV   ++ W+ E + YN  +  CS    C HYTQVVWAKT+  GCG   C K    ++     L CNY P GN   ++ Y+ G  CS+CP+G  C + LC
Sbjct:   28 LTDEEKRLMVELHNLYRAQVS-------PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENL-FAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLC 197          
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 112.849 bits (281), Expect = 1.510e-28
Identity = 63/185 (34.05%), Postives = 100/185 (54.05%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD---DVTEGIKMWYDEVKGYNFQSEKCSV-STCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T ++K+ +++ HN YR +V+       P A++ML ++W  E  A A+ +  +CV+ H+    R+       G+N+ +  +D   DV   ++ W+ E + YN  +  CS    C HYTQVVWAKT+  GCG   C K    ++     L CNY P GN   ++ Y+ G  CS+CP+G  C + LC
Sbjct:   28 LTDEEKRLMVELHNLYRAQVS-------PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENL-FAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLC 197          
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 112.849 bits (281), Expect = 1.510e-28
Identity = 63/185 (34.05%), Postives = 100/185 (54.05%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD---DVTEGIKMWYDEVKGYNFQSEKCSV-STCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T ++K+ +++ HN YR +V+       P A++ML ++W  E  A A+ +  +CV+ H+    R+       G+N+ +  +D   DV   ++ W+ E + YN  +  CS    C HYTQVVWAKT+  GCG   C K    ++     L CNY P GN   ++ Y+ G  CS+CP+G  C + LC
Sbjct:   28 LTDEEKRLMVELHNLYRAQVS-------PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENL-FAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLC 197          
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI15 (peptidase inhibitor 15 [Source:HGNC Symbol;Acc:HGNC:8946])

HSP 1 Score: 108.227 bits (269), Expect = 4.410e-28
Identity = 65/196 (33.16%), Postives = 91/196 (46.43%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYW--HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++ +   A++D HN+ R     GKV   P AANM  + W       A+ +   C++ H  S   +F     +GQN++        + + +K WYDEVK Y F            +C    C HYTQ+VWA +   GC +  C    +        ++L CNYAP GNW  E  Y+VG  CS CP   G  C D LC
Sbjct:   62 ISQNDMIAILDYHNQVR-----GKVF--PPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYAFPYPQDCNPRCPMRCFGPMCTHYTQMVWATSNRIGCAIHTCQNMNVWGSVWRRAVYLVCNYAPKGNWIGEAPYKVGVPCSSCPPSYGGSCTDNLC 245          
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI15 (peptidase inhibitor 15 [Source:HGNC Symbol;Acc:HGNC:8946])

HSP 1 Score: 108.227 bits (269), Expect = 4.410e-28
Identity = 65/196 (33.16%), Postives = 91/196 (46.43%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYW--HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++ +   A++D HN+ R     GKV   P AANM  + W       A+ +   C++ H  S   +F     +GQN++        + + +K WYDEVK Y F            +C    C HYTQ+VWA +   GC +  C    +        ++L CNYAP GNW  E  Y+VG  CS CP   G  C D LC
Sbjct:   62 ISQNDMIAILDYHNQVR-----GKVF--PPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYAFPYPQDCNPRCPMRCFGPMCTHYTQMVWATSNRIGCAIHTCQNMNVWGSVWRRAVYLVCNYAPKGNWIGEAPYKVGVPCSSCPPSYGGSCTDNLC 245          
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: scl-5 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q18543])

HSP 1 Score: 112.464 bits (280), Expect = 1.289e-30
Identity = 68/216 (31.48%), Postives = 105/216 (48.61%), Query Frame = 2
Query:   26 MNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQG----KVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YWHSDDVTE-------GIKMWYDEVKGYNFQSEKCSVST----CLHYTQVVWAKTKEFGCGVADCSKKKLG--KIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCN--DGLCS 613
            M+  ++ +       +   + + + A+++ HN  R  +A+G    K + +P A++ML++KW +   A AQ + NKC   H  +          +G+N+  YW S  +T        G   W  E + Y + S   S+S       H TQ+ WAKT   GCGV +C K   G  K+ + C Y P GN+ N+  Y  G  CSKCP+GT C    GLC+
Sbjct:    1 MHTSILLLTALCAGAYAQFSANGQAAILNVHNTLRSRIAKGTYVAKGTAKPAASDMLKMKWDATVAASAQAYANKCPTGHSGAAG--------LGENLYWYWTSATITNIDQFGATGSAAWEKEFQDYGWSSNTLSMSLFNTGIGHATQMAWAKTNLIGCGVKNCGKDTNGFNKVTVVCQYKPQGNYLNQNIYTSGTTCSKCPSGTSCEAATGLCA 208          
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: scl-2 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q20603])

HSP 1 Score: 106.301 bits (264), Expect = 2.896e-28
Identity = 68/215 (31.63%), Postives = 105/215 (48.84%), Query Frame = 2
Query:   26 MNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQG----KVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFG-SYQWVGQNIAYWHSDDV-------TEGIKMWYDEVKGYNFQSEKCSV----STCLHYTQVVWAKTKEFGCGVADCSKKK--LGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKC--NDGLC 610
            MN+ L+ VA+ +  C  +     +  +I+AHN  R ++A+G    K +++    N+L++KW S   A AQ + N C   H  SG+   G +  W      YW S  +       +     W  E + Y ++S   ++    +   H TQ+ WAK+   GCGV DC +    L K+ + C Y P GN+ N+  Y  G  CS CP+GT C  + GLC
Sbjct:    1 MNYLLLVVALAVG-CSADFGSSGQNGIINAHNTLRSKIAKGTYVAKGTQKSPGTNLLKMKWDSAVAASAQNYANGCPTGH--SGDAGLGENLYW------YWTSGSLGDLNQYGSAASASWEKEFQDYGWKSNLMTIDLFNTGIGHATQMAWAKSNLIGCGVKDCGRDSNGLNKVTVVCQYKPQGNFINQYIYVSGATCSGCPSGTSCETSTGLC 206          
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: scl-2 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q20603])

HSP 1 Score: 106.301 bits (264), Expect = 2.896e-28
Identity = 68/215 (31.63%), Postives = 105/215 (48.84%), Query Frame = 2
Query:   26 MNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQG----KVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFG-SYQWVGQNIAYWHSDDV-------TEGIKMWYDEVKGYNFQSEKCSV----STCLHYTQVVWAKTKEFGCGVADCSKKK--LGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKC--NDGLC 610
            MN+ L+ VA+ +  C  +     +  +I+AHN  R ++A+G    K +++    N+L++KW S   A AQ + N C   H  SG+   G +  W      YW S  +       +     W  E + Y ++S   ++    +   H TQ+ WAK+   GCGV DC +    L K+ + C Y P GN+ N+  Y  G  CS CP+GT C  + GLC
Sbjct:    1 MNYLLLVVALAVG-CSADFGSSGQNGIINAHNTLRSKIAKGTYVAKGTQKSPGTNLLKMKWDSAVAASAQNYANGCPTGH--SGDAGLGENLYW------YWTSGSLGDLNQYGSAASASWEKEFQDYGWKSNLMTIDLFNTGIGHATQMAWAKSNLIGCGVKDCGRDSNGLNKVTVVCQYKPQGNFINQYIYVSGATCSGCPSGTSCETSTGLC 206          
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: scl-10 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q20609])

HSP 1 Score: 104.375 bits (259), Expect = 1.511e-27
Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query:   20 MKMNFKLVFVAIYITICH--CNVTPDQKKALIDAHNKYRREVAQGKV----SKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTE---------GIKMWYDEVKGY----NFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLA-CNYAPGGNWNNEKAYEVGKACSKCPTGTKC--NDGLC 610
            M   F+L+ + I+ + C   C  +   K  ++  HN  R ++A GK     S +P A+NM++L W +  E  AQ +   C   H +S          +G+N+ +W S  VT+            +W  E + +    N  +E+   S   H TQ+ WA T + GCG++ CS    G  ++  C Y+P GN+     Y+ G+ CS CP GT C  + GLC
Sbjct:    1 MNCYFRLICLLIF-SFCETLCEFSETGKNYILSRHNYLRSQIALGKYVAGNSTKPSASNMMKLIWDTTLETTAQDYSTGCPTGHSASRAN-------IGENMYWWTSPVVTQTDAELLGNRSANLWESEFQRFGWNGNLLTEELFNSGIGHATQMAWATTNKIGCGISKCSSDSFGTQYVVVCLYSPAGNYIGMDIYKSGETCSNCPDGTNCESSTGLC 211          
BLAST of Venom allergen 5 vs. Ensembl Celegans
Match: scl-14 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q93444])

HSP 1 Score: 98.5969 bits (244), Expect = 2.462e-25
Identity = 64/213 (30.05%), Postives = 100/213 (46.95%), Query Frame = 2
Query:   41 VFVAIYITIC---HCNVTPDQKKALIDAHNKYRREVAQG----KVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YWHSDDVTE-------GIKMWYDEVKGYNFQSEKCSVST----CLHYTQVVWAKTKEFGCGVADCSKKKLG--KIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCN--DGLC 610
             F+ +   +C   +   T + + A+++ HN  R  +A+G    + + +  A++ML++KW       +Q + N+C   H +      G    VG+N+  YW S  +T            W  E + Y + S   ++S       H TQ+ WAKT   GCGV +C     G  K+ + C+Y P GN+ N+  Y  G  CSKCP GT C    GLC
Sbjct:    3 TFILLLTVLCAGAYTQFTANGQAAILNVHNTLRSRIAKGTYVARGTVKHAASDMLKMKWLRSLATSSQIYANRCPTGHSN----MIG----VGENLYWYWTSGTITNIDQFGAMASAAWEKEFQDYGWSSNTLTMSLFNSGVGHATQMAWAKTNLIGCGVKNCGMDTNGMNKVAVVCHYQPQGNYLNQNIYTSGTTCSKCPAGTSCEAATGLC 207          
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG8483 (gene:FBgn0038126 transcript:FBtr0082782)

HSP 1 Score: 103.99 bits (258), Expect = 6.127e-26
Identity = 65/179 (36.31%), Postives = 97/179 (54.19%), Query Frame = 2
Query:   65 ICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YWHS-------DDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADC-SKKKLGKIFLACNYAPGGNWNNEKAYEVGK-ACS 571
            I    +T +++  ++  HN+ R+ VA G+   QP A NM E+ W  E  A+AQK+ + C F+HD    R    +  +GQN+A  W +        D    I+ W++EV+ Y+F  +  S  T  HY+Q+VW +T   GCG A+     K  K+++ CNY PGGN      YEVGK +CS
Sbjct:   27 IIASGITAEERSIILQEHNRLRQIVATGRYPGQPGAENMREIVWDDELAARAQKWADNCQFRHDP--HRTINRFT-MGQNLAIIWSTAPLDADDGDFPSRIQSWFNEVQKYSF-GDAWSPKTG-HYSQLVWGETSLVGCGYAEYKDTSKYNKLYV-CNYGPGGNVVGYNPYEVGKPSCS 199          
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: Ag5r (gene:FBgn0015010 transcript:FBtr0333658)

HSP 1 Score: 75.485 bits (184), Expect = 4.120e-16
Identity = 57/187 (30.48%), Postives = 81/187 (43.32%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA---YWHSDDVTE----GIKMWYDE--------VKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVA--DCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGT 589
            ++  +K AL+   N+YR  +A G  +    A  M  +KW+ E    A   V  C  KHD  G     ++ W GQN+A   Y++  +VT     G+ MWYDE        +  Y       ++    H+T +V  +  E GC  A    S +      LACNYA       +      KA SKC TGT
Sbjct:   54 LSSAEKDALVARTNEYRNHIAGGLNANLSAACRMATIKWNDELAYLASLNVKSCQMKHD--GCHNTDAFDWSGQNLAWMGYYNPLNVTHYLEWGVDMWYDEAVYTKQAYIDAYPSNYNGPAIG---HFTVLVADRNTEVGCAAATYSVSGQSYKAFLLACNYAATNVLGIKMYSSCSKAASKCTTGT 235          
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: Ag5r (gene:FBgn0015010 transcript:FBtr0073937)

HSP 1 Score: 75.485 bits (184), Expect = 4.120e-16
Identity = 57/187 (30.48%), Postives = 81/187 (43.32%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA---YWHSDDVTE----GIKMWYDE--------VKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVA--DCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGT 589
            ++  +K AL+   N+YR  +A G  +    A  M  +KW+ E    A   V  C  KHD  G     ++ W GQN+A   Y++  +VT     G+ MWYDE        +  Y       ++    H+T +V  +  E GC  A    S +      LACNYA       +      KA SKC TGT
Sbjct:   54 LSSAEKDALVARTNEYRNHIAGGLNANLSAACRMATIKWNDELAYLASLNVKSCQMKHD--GCHNTDAFDWSGQNLAWMGYYNPLNVTHYLEWGVDMWYDEAVYTKQAYIDAYPSNYNGPAIG---HFTVLVADRNTEVGCAAATYSVSGQSYKAFLLACNYAATNVLGIKMYSSCSKAASKCTTGT 235          
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: Ag5r (gene:FBgn0015010 transcript:FBtr0305582)

HSP 1 Score: 75.485 bits (184), Expect = 4.120e-16
Identity = 57/187 (30.48%), Postives = 81/187 (43.32%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA---YWHSDDVTE----GIKMWYDE--------VKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVA--DCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGT 589
            ++  +K AL+   N+YR  +A G  +    A  M  +KW+ E    A   V  C  KHD  G     ++ W GQN+A   Y++  +VT     G+ MWYDE        +  Y       ++    H+T +V  +  E GC  A    S +      LACNYA       +      KA SKC TGT
Sbjct:   54 LSSAEKDALVARTNEYRNHIAGGLNANLSAACRMATIKWNDELAYLASLNVKSCQMKHD--GCHNTDAFDWSGQNLAWMGYYNPLNVTHYLEWGVDMWYDEAVYTKQAYIDAYPSNYNGPAIG---HFTVLVADRNTEVGCAAATYSVSGQSYKAFLLACNYAATNVLGIKMYSSCSKAASKCTTGT 235          
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG32679 (gene:FBgn0052679 transcript:FBtr0071469)

HSP 1 Score: 69.707 bits (169), Expect = 4.221e-14
Identity = 55/187 (29.41%), Postives = 79/187 (42.25%), Query Frame = 2
Query:  104 LIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQN--IAYWHSDDVT----EGIKMWYDE----VKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSK-KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCN-DGLCS*SKIY 628
            ++  HN+ R  +A G VS  P A  M  + W +     A     +C   HD    R   +Y++ GQN  I +  S DV     + I  W+DE      G     +        H+T +V  +    GC +A  +    +    LACNYA   N  N   Y  G A S+C TG   N   LCS +++Y
Sbjct:   64 ILQLHNERRNLIAGGGVSGFPSAVQMATMSWDTTLAQLAAYNALQCRMAHDEC--RNTNTYRYAGQNLSILFTRSVDVAVFLRQRIAAWFDENRDATSGDMEDYQMRGGPAIGHFTTMVNERNNRVGCAIARFTDANNVQATLLACNYAV-TNVVNNPVYRAGTAASECTTGRNSNYPNLCSPNEVY 247          
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275])

HSP 1 Score: 105.916 bits (263), Expect = 1.914e-27
Identity = 63/187 (33.69%), Postives = 95/187 (50.80%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD--DVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKK-KLGKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++     AL+D HN+ R +V        P AANM  + W       A+ + ++C+++H   G   F   Q +GQN++        + + +K WYDE   +++ S +CS S C HYTQ+VWA + + GC +  CS     G +      L CNYA  GNW  E  Y++G+ CS CP+  G  CN   C
Sbjct:   61 ISGKDMTALLDYHNRVRSQVF-------PPAANMEYMVWDERLAKSAEFWASQCIWEH---GPHHF--LQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPS-RCSGSVCTHYTQMVWAASNKIGCAIKKCSDIFVFGSMWKQATLLVCNYAIKGNWVGEAPYKIGRPCSACPSSYGGSCNKNQC 234          
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275])

HSP 1 Score: 105.916 bits (263), Expect = 1.914e-27
Identity = 63/187 (33.69%), Postives = 95/187 (50.80%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD--DVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKK-KLGKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++     AL+D HN+ R +V        P AANM  + W       A+ + ++C+++H   G   F   Q +GQN++        + + +K WYDE   +++ S +CS S C HYTQ+VWA + + GC +  CS     G +      L CNYA  GNW  E  Y++G+ CS CP+  G  CN   C
Sbjct:   61 ISGKDMTALLDYHNRVRSQVF-------PPAANMEYMVWDERLAKSAEFWASQCIWEH---GPHHF--LQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPS-RCSGSVCTHYTQMVWAASNKIGCAIKKCSDIFVFGSMWKQATLLVCNYAIKGNWVGEAPYKIGRPCSACPSSYGGSCNKNQC 234          
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-130131-1])

HSP 1 Score: 107.071 bits (266), Expect = 1.547e-26
Identity = 65/191 (34.03%), Postives = 92/191 (48.17%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YW-HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++ ++  HNK R EV        P A+NM  + W  E E  A  +  +C ++H   G +       +GQN+A +W         ++ WYDEVK Y +           E+CS   C HYTQ+VWA T   GC V  C +  +        ++L CNY+P GNW  E  Y+ G+ CS+CP   G  C D LC
Sbjct:   65 REEILKLHNKLRGEV-------YPTASNMEYMIWDDELERSATSWAEQCQWEH---GPQDL--LMSIGQNLAVHWGRYRSPAYHVQAWYDEVKDYTYPYPHECNPWCPERCSGPMCTHYTQLVWATTNRVGCAVHVCPRMNVWGEIWENAVYLVCNYSPKGNWIGEAPYQHGRPCSQCPPSYGGVCRDNLC 243          
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 106.686 bits (265), Expect = 1.861e-26
Identity = 65/196 (33.16%), Postives = 97/196 (49.49%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYW-HSDDVTEGIKMWYDEVKGYNFQSE---------KCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL-GKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            +T    +A++D HNK R +V        P A+NM  + W +E E  A+++   C+++H  +G         +GQN+  +W      T  ++ WYDEVK Y+F            +CS   C HYTQ+VWA +   GC +  C    + G+I     +L CNY+P GNW     Y+ G +CS CP   G  C + LC
Sbjct:   62 ITASDMQAILDLHNKLRGQV-------YPPASNMEYMVWDNELERSAEEWAETCLWEHGPAGL-----LPQIGQNLGVHWGRYRPPTSHVQAWYDEVKDYSFPYPQECNPHCPFRCSGPVCTHYTQLVWATSSRIGCAINVCYNMNVWGQIWAKAVYLVCNYSPKGNWWGYAPYKHGTSCSACPPSYGGVCRENLC 245          
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 106.686 bits (265), Expect = 2.100e-26
Identity = 65/196 (33.16%), Postives = 97/196 (49.49%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYW-HSDDVTEGIKMWYDEVKGYNFQSE---------KCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL-GKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            +T    +A++D HNK R +V        P A+NM  + W +E E  A+++   C+++H  +G         +GQN+  +W      T  ++ WYDEVK Y+F            +CS   C HYTQ+VWA +   GC +  C    + G+I     +L CNY+P GNW     Y+ G +CS CP   G  C + LC
Sbjct:   62 ITASDMQAILDLHNKLRGQV-------YPPASNMEYMVWDNELERSAEEWAETCLWEHGPAG-----LLPQIGQNLGVHWGRYRPPTSHVQAWYDEVKDYSFPYPQECNPHCPFRCSGPVCTHYTQLVWATSSRIGCAINVCYNMNVWGQIWAKAVYLVCNYSPKGNWWGYAPYKHGTSCSACPPSYGGVCRENLC 245          
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:613091])

HSP 1 Score: 110.153 bits (274), Expect = 1.877e-27
Identity = 64/191 (33.51%), Postives = 94/191 (49.21%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YW-HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            K+ +I  HNK R +V        P A+NM  + W  E E  A+ +  +C+++H  +          +GQN+A +W         ++ WYDEVK Y +           E+CS   C HYTQ+VWA T + GC V  C +  +        ++L CNY+P GNW  E  Y+ G+ CS+CP   G  C + LC
Sbjct:   57 KEEIIQLHNKLRGQV-------HPSASNMEYMTWDDELEKSAEAWAEECIWEHGPTA-----LLMSIGQNLAVHWGRYRQPAYHVQSWYDEVKDYTYPYPHECNPYCPERCSGPMCTHYTQIVWATTTKVGCAVNVCKRMNVWGDIWENAVYLVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGNCQNNLC 235          
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:613091])

HSP 1 Score: 110.153 bits (274), Expect = 2.070e-27
Identity = 64/191 (33.51%), Postives = 94/191 (49.21%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YW-HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            K+ +I  HNK R +V        P A+NM  + W  E E  A+ +  +C+++H  +          +GQN+A +W         ++ WYDEVK Y +           E+CS   C HYTQ+VWA T + GC V  C +  +        ++L CNY+P GNW  E  Y+ G+ CS+CP   G  C + LC
Sbjct:   57 KEEIIQLHNKLRGQV-------HPSASNMEYMTWDDELEKSAEAWAEECIWEHGPTA-----LLMSIGQNLAVHWGRYRQPAYHVQSWYDEVKDYTYPYPHECNPYCPERCSGPMCTHYTQIVWATTTKVGCAVNVCKRMNVWGDIWENAVYLVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGNCQNNLC 235          
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:613091])

HSP 1 Score: 109.768 bits (273), Expect = 2.317e-27
Identity = 64/191 (33.51%), Postives = 94/191 (49.21%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YW-HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            K+ +I  HNK R +V        P A+NM  + W  E E  A+ +  +C+++H  +          +GQN+A +W         ++ WYDEVK Y +           E+CS   C HYTQ+VWA T + GC V  C +  +        ++L CNY+P GNW  E  Y+ G+ CS+CP   G  C + LC
Sbjct:   57 KEEIIQLHNKLRGQV-------HPSASNMEYMTWDDELEKSAEAWAEECIWEHGPTAL-----LMSIGQNLAVHWGRYRQPAYHVQSWYDEVKDYTYPYPHECNPYCPERCSGPMCTHYTQIVWATTTKVGCAVNVCKRMNVWGDIWENAVYLVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGNCQNNLC 235          
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 107.071 bits (266), Expect = 1.310e-26
Identity = 65/196 (33.16%), Postives = 90/196 (45.92%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYW-HSDDVTEGIKMWYDEVKGYNFQSE---------KCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            +T    K ++D HNK R EV        P A+NM  + W  E E  A+ +   C+++H  +          +GQN+ A+W      T  ++ WYDEV+ Y F            +CS   C HYTQ+VWA +   GC +  C    +        I+L CNY+P GNW     Y+ G  CS CP   G  C D LC
Sbjct:   57 ITESDMKLILDLHNKLRGEV-------YPPASNMEFMIWDVELERSAEAWAETCLWEHGPADLLPV-----IGQNLGAHWGRYRPPTYHVQAWYDEVRDYTFPYPQECDPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMNVWGQIWPKAIYLVCNYSPKGNWWGHAPYKHGHPCSACPPSYGGGCKDNLC 240          
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: ENSXETT00000036788.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464547.1:593:5826:1 gene:ENSXETG00000015856.1 transcript:ENSXETT00000036788.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 106.301 bits (264), Expect = 1.592e-26
Identity = 62/181 (34.25%), Postives = 94/181 (51.93%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWH--SDDVTEGIKMWYDEVKGYNFQSEKCSV-STCLHYTQVVWAKTKEFGCGVADCSKKK----LGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            ++K+ ++D HN YR +        +P A++M++L W S  EA A+ +  KC+++H+   +R F     +G+N+      S DV  G+  W+ E   YNF +  C     C HYTQ+VWA T+  GCG   C K +         L CNY P GN+  E  Y+ G  C++CP+   C   LC
Sbjct:   33 EEKRIILDKHNFYRSQ-------TEPSASDMIKLTWDSALEAMAKSYAEKCIWEHNK--DRGF-----IGENLFVMSGSSLDVALGLDDWHKERGYYNFTTGMCQEGQMCGHYTQMVWAGTERVGCGQNFCPKLEGVDDENMYLLVCNYEPPGNFEGESPYKEGSRCTECPSDHVCIGSLC 199          
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 116.701 bits (291), Expect = 1.080e-31
Identity = 64/184 (34.78%), Postives = 98/184 (53.26%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD--DVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T D+K+ ++D HN+YR +V+       P A++ML+++W  E  A A+ +  KCV+ H+    R+       G+N+     +  DV   +  W++E + YNF +  C  +  C HYTQVVW+KT+  GCG   C      ++     L CNY P GN    K Y+ G  CS+CP G  C + LC
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR-------GENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYSCENSLC 199          
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 117.087 bits (292), Expect = 1.644e-31
Identity = 64/184 (34.78%), Postives = 98/184 (53.26%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD--DVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T D+K+ ++D HN+YR +V+       P A++ML+++W  E  A A+ +  KCV+ H+    R+       G+N+     +  DV   +  W++E + YNF +  C  +  C HYTQVVW+KT+  GCG   C      ++     L CNY P GN    K Y+ G  CS+CP G  C + LC
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR-------GENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYSCENSLC 199          
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 117.087 bits (292), Expect = 1.644e-31
Identity = 64/184 (34.78%), Postives = 98/184 (53.26%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD--DVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T D+K+ ++D HN+YR +V+       P A++ML+++W  E  A A+ +  KCV+ H+    R+       G+N+     +  DV   +  W++E + YNF +  C  +  C HYTQVVW+KT+  GCG   C      ++     L CNY P GN    K Y+ G  CS+CP G  C + LC
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR-------GENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYSCENSLC 199          
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 117.087 bits (292), Expect = 3.871e-30
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD---DVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T D+K+ ++D HN+YR +V+       P A++ML+++W  E  A A+ +  KCV+ H+    R+       G+N+ +  +D   DV   +  W++E + YNF +  C  +  C HYTQVVW+KT+  GCG   C      ++     L CNY P GN    K Y+ G  CS+CP G  C + LC
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR-------GENL-FAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYSCENSLC 199          
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi15 (peptidase inhibitor 15 [Source:MGI Symbol;Acc:MGI:1934659])

HSP 1 Score: 107.842 bits (268), Expect = 5.170e-28
Identity = 65/196 (33.16%), Postives = 91/196 (46.43%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYW--HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++ +   A++D HN+ R     GKV   P AANM  + W       A+ +   C++ H  S   +F     +GQN++        + + +K WYDEVK Y F            +C    C HYTQ+VWA +   GC +  C    +        ++L CNYAP GNW  E  Y+VG  CS CP   G  C D LC
Sbjct:   73 ISQNDMIAILDYHNQVR-----GKVF--PPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYAFPYPQDCNPRCPMRCFGPMCTHYTQMVWATSNRIGCAIHTCQNMNVWGSVWRRAVYLVCNYAPKGNWIGEAPYKVGVPCSSCPPSYGGACTDNLC 256          
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPU1|VA576_SCODE (Scoloptoxin SSD976 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 1.630e-34
Identity = 69/180 (38.33%), Postives = 95/180 (52.78%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYW-HSDDVTEGIKMWYDEVKGYN---FQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCN-DGLCS 613
            + KK ++D HNK R+ VA G+ S QP AANM EL W+ E  A AQ+    CVF+H +   RK   Y ++G+NI    + D +   +  WYDEVK       +S         HYTQ+VWA T+  GCG+   S +     +L C Y PGGN+ ++  Y+ G   S C  G      GLC+
Sbjct:   34 NMKKMILDLHNKKRQIVANGQQSGQPSAANMKELHWNDEIAANAQRSAETCVFEHTAKSLRKTTKYSYLGENIYKGSYPDPIPRSVNAWYDEVKDVTPAVVKSFSSGGPMIGHYTQMVWANTEALGCGLVTASDR---NSYLFCQYGPGGNYRSQPIYKQGPPASDCKNGKSSKYPGLCN 210          
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPU0|VA552_SCODE (Scoloptoxin SSD552 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 1.894e-34
Identity = 71/183 (38.80%), Postives = 97/183 (53.01%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYWHSDDVTEGIKMWYDEVKGY------NFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCN-DGLCS 613
            + KK ++D HNK R+ VA G+ S QP AANM EL W  E EA AQ+    CVFKH +   RK   Y ++G+N+    + D +   +  WYDEVK        +F  +   +    HYTQ+VWA T+  GCG+   S    G  +L C Y PGGN+  ++ Y+ G   S C  G      GLC+
Sbjct:   34 NMKKMILDLHNKKRQIVANGQQSGQPLAANMKELHWDDEIEAIAQRSAETCVFKHTAKSLRKSSKYSYLGENVYKGGYPDPIPRSVNKWYDEVKDVTPAVVKSFSDDGPMIG---HYTQMVWANTEALGCGLVTASD---GTSYLFCQYGPGGNYPGQQIYKQGPPGSGCKNGKSSKYPGLCN 210          
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPV2|VA558_SCODE (Scoloptoxin SSD558 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.711e-33
Identity = 68/180 (37.78%), Postives = 94/180 (52.22%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYW-HSDDVTEGIKMWYDEVKGYN---FQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCN-DGLCS 613
            + KK ++D HNK R+ VA G+ S QP AANM EL W+ E  A AQ+    CVF+H +   RK   Y ++G+NI    + D +   +  WYDEVK       +S         HYTQ+VWA T+   CG+   S +     +L C Y PGGN+ ++  Y+ G   S C  G      GLC+
Sbjct:   34 NMKKMILDLHNKKRQIVANGQQSGQPSAANMKELHWNDEIAANAQRSAETCVFEHTAKSLRKTTKYSYLGENIYKGSYPDPIPRSVNAWYDEVKDVTPAVVKSFSSGGPMIGHYTQMVWANTEALDCGLVTASDR---NSYLFCQYGPGGNYRSQPIYKQGPPASDCKNGKSSKYPGLCN 210          
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|Q7Z156|VA5_POLSR (Venom allergen 5 OS=Polybia scutellaris rioplatensis OX=260979 PE=1 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 2.276e-29
Identity = 70/176 (39.77%), Postives = 98/176 (55.68%), Query Frame = 2
Query:   59 ITICHCNVTPDQKKALIDAHNKYRREVAQGKVSK-----QPEAANMLELKWSSEAEAKAQKFVNKC-VFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIK----MWYDEVKGYNFQS--EKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAY 550
            ++I    VT ++KK ++D HN++R++VAQG  ++     QP A++M  L W+ E    AQ + N+C  F HD    R    YQ VGQNIAY  S     GI     +W +EVK +N+     K + +   HYTQVVWAKTKE GCG     +K +   +L CNY P GN+  +  Y
Sbjct:   32 VSITSVGVTEEEKKLIVDEHNRFRQKVAQGLETRGNPGPQPAASDMNNLVWNDELAYIAQVWANQCQFFVHDKC--RNTAQYQ-VGQNIAYSASTAAYPGIVSLIVLWENEVKDFNYSQGITKENFAKVGHYTQVVWAKTKEVGCGSIKYIEKGMKSHYLVCNYGPAGNYMGQPIY 204          
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|Q9ET66|PI16_MOUSE (Peptidase inhibitor 16 OS=Mus musculus OX=10090 GN=Pi16 PE=1 SV=2)

HSP 1 Score: 117.087 bits (292), Expect = 2.708e-29
Identity = 65/185 (35.14%), Postives = 100/185 (54.05%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSD---DVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADCSK----KKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLC 610
            +T D+K+ ++D HN+YR +V+       P A++ML+++W  E  A A+ +  KCV+ H+    R+       G+N+ +  +D   DV   +  W++E + YNF +  C  +  C HYTQVVW+KT+  GCG   C      ++     L CNY P GN    K Y+ G  CS+CP G  C + LC
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR-------GENL-FAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYSCENSLC 199          
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A564YFQ7 (SCP domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS5585 PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 3.284e-47
Identity = 74/152 (48.68%), Postives = 104/152 (68.42%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAY 550
            K+ L+D  N+YRREVA G+V  QP ++ + +LKW++E EA AQ+  ++C F+HDSS +RK   + WVGQNIAY  S  + + +KMW++E K YN+    CS   C HYTQ+VWA T + GCGV+ C+      +F+ CNY PGGN+NN+K Y
Sbjct:   53 KQELLDLQNQYRREVAAGRVPNQPASSKIRDLKWNTELEASAQRHADRCRFQHDSSDDRKTAEFWWVGQNIAY--SSSIAQNVKMWFEEHKDYNYSHNYCS-GVCGHYTQLVWANTTDVGCGVSKCNFSGFNAVFVVCNYGPGGNFNNQKPY 201          
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A1C9ZZV5 (SCP domain-containing protein (Fragment) OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 3.700e-46
Identity = 81/201 (40.30%), Postives = 116/201 (57.71%), Query Frame = 2
Query:   26 MNFKLVFVAIYITICHCNVTPDQ-KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKI----FLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 613
            M F L  +  +I+I     TP + K  L+ AHN  RR++A GK   QP AANM+E+ W      KA+++  KC+  HD+  +R+   + +VGQN+  +   +  E ++ WYDE K Y ++ + CS + C HYTQV W+K+   GC   +C +K  G         CNY P GN+N+E  YEVG ACSKCP GT C + LC+
Sbjct:    7 MQFLLCGIISFISIALIFCTPPEYKDFLLKAHNDLRRDIALGKTPNQPAAANMIEMVWDDALAGKAEEWAGKCIAGHDTYDDRQLDKFMFVGQNM--FAGSNYKEVVQGWYDEYKDYTYEGKGCS-AVCGHYTQVAWSKSYAVGCAAVNCKEKTGGSFAYGWLFICNYGPAGNFNDEAPYEVGAACSKCPKGTSCKNNLCA 204          
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A5K3ENG8 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.525e-45
Identity = 71/158 (44.94%), Postives = 104/158 (65.82%), Query Frame = 2
Query:   77 NVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAY 550
            +V   +K  L++ HNKYRR V+ G++ +QP ++ + +LKWS+E EA AQ+  + C F HDS+  R+   + WVGQNIAY  S  + + ++MW+DE K YN+QS  CS   C HYTQ+VWA T   GCGV+ C+ +    +++ CNY PGGN+N  + Y
Sbjct:   51 SVLSAEKSELLNLHNKYRRLVSDGQIREQPSSSKIHDLKWSTELEASAQRHADTCRFAHDSADARRTPQWDWVGQNIAY--SSSIAKNVEMWFDEYKEYNYQSNYCS-GVCGHYTQLVWANTTHVGCGVSKCNFRGFNAVYVVCNYGPGGNYNGRRPY 205          
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A564Z3X2 (SCP domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS12343 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 2.479e-42
Identity = 73/152 (48.03%), Postives = 102/152 (67.11%), Query Frame = 2
Query:   98 KALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAY 550
            + ++D HN+YRR+VA GKVS QP  +N+ +LKW+SE   KAQ++ NKC+  HDS   R+   +++VGQNIAY  + DV +GIK W+DE   +N+ + KC+    C HYTQ+VWAKT + GCG+  C  K    + L CNY   GN+NNE+ Y
Sbjct:  105 QIVLDLHNEYRRQVATGKVSGQPAGSNIHDLKWNSELAKKAQEWANKCIPDHDSKTARRTSQFRYVGQNIAY--NSDVQKGIKEWFDEHVNFNYYANKCAYGKVCGHYTQLVWAKTTDLGCGINMCDYKGFKLVNLVCNYGTAGNFNNERPY 254          
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A0R3SFR1 (SCP domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS3694 PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 3.573e-42
Identity = 68/143 (47.55%), Postives = 96/143 (67.13%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPG 523
            K+ L+D  N+YRREVA G+V  QP ++ + +LKW++E EA AQ+  ++C F+HDSS +RK   + WVGQNIAY  S  + + +KMW++E K YN+    CS   C HYTQ+VWA T + GCGV+ C+      +F+ CNY PG
Sbjct:   53 KQELLDLQNQYRREVAAGRVPNQPASSKIRDLKWNTELEASAQRHADRCRFQHDSSDDRKTAEFWWVGQNIAY--SSSIAQNVKMWFEEHKDYNYSHNYCS-GVCGHYTQLVWANTTDVGCGVSKCNFSGFNAVFVVCNYGPG 192          
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: ENSAMXT00000036030.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 110.153 bits (274), Expect = 3.015e-29
Identity = 66/195 (33.85%), Postives = 104/195 (53.33%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADC-SKKKLG---KIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTK-CNDGLCS*SKIYILIKRIETE 655
            DQ++ +++ HN +R  V       +P AA+M+ +KWS E    A+++  KC+++H+   +   G   ++          D+ + +  W+ E K Y++ +  C+ +  C HYTQ+VWAKTKE GCG   C + + LG      L CNY P GN+  +K Y+VG+ CSKCP   K C D  C  +    L   + TE
Sbjct:   29 DQQR-IVNLHNHHRSTV-------EPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNIMGENLFITTGPL-----DIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLCETVEALGFKNATLLVCNYLPLGNYMGQKPYQVGEQCSKCPDDLKNCVDQACDSTDRDELPSMMPTE 210          
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: ENSAMXT00000031557.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 110.153 bits (274), Expect = 3.976e-29
Identity = 63/180 (35.00%), Postives = 99/180 (55.00%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADC-SKKKLG---KIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTK-CNDGLC 610
            DQ++ +++ HN +R  V       +P AA+M+ +KWS E    A+++  KC+++H+   +   G   ++          D+ + +  W+ E K Y++ +  C+ +  C HYTQ+VWAKTKE GCG   C + + LG      L CNY P GN+  +K Y+VG+ CSKCP   K C D  C
Sbjct:   29 DQQR-IVNLHNHHRSTV-------EPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNIMGENLFITTGPL-----DIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLCETVEALGFKNATLLVCNYLPLGNYMGQKPYQVGEQCSKCPDDLKNCVDQAC 195          
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: ENSAMXT00000029824.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 110.153 bits (274), Expect = 5.515e-29
Identity = 63/180 (35.00%), Postives = 99/180 (55.00%), Query Frame = 2
Query:   89 DQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVS-TCLHYTQVVWAKTKEFGCGVADC-SKKKLG---KIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTK-CNDGLC 610
            DQ++ +++ HN +R  V       +P AA+M+ +KWS E    A+++  KC+++H+   +   G   ++          D+ + +  W+ E K Y++ +  C+ +  C HYTQ+VWAKTKE GCG   C + + LG      L CNY P GN+  +K Y+VG+ CSKCP   K C D  C
Sbjct:   29 DQQR-IVNLHNHHRSTV-------EPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNIMGENLFITTGPL-----DIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLCETVEALGFKNATLLVCNYLPLGNYMGQKPYQVGEQCSKCPDDLKNCVDQAC 195          
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: r3hdml (R3H domain containing like [Source:NCBI gene;Acc:103046222])

HSP 1 Score: 105.531 bits (262), Expect = 1.896e-27
Identity = 64/191 (33.51%), Postives = 93/191 (48.69%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQN--IAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLCS*SK 622
            V+     AL++ HN+ R +V        P AANM  + W       A+ + ++C++ H  S   K+     +GQN  I+      V E ++ W+DE   +++   +C+ S C HYTQ+VWA T   GC +  CS   +          L CNY+  GNW  E  Y+ GK CS CP+  G  CN   CS SK
Sbjct:   62 VSSRDMTALLEYHNRVRSQVF-------PPAANMEYMVWDERLAKSAESWASQCIWDHGPSHVMKY-----MGQNLSISSGRYRSVIELVRSWHDEKNSFSYP-NRCTGSVCSHYTQMVWASTNRIGCAINRCSNMYVFGSTWKQATLLVCNYSIKGNWVGEAPYKTGKPCSACPSVYGGSCNKNQCSTSK 239          
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-130131-1])

HSP 1 Score: 107.071 bits (266), Expect = 1.168e-26
Identity = 66/191 (34.55%), Postives = 91/191 (47.64%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YW-HSDDVTEGIKMWYDEVKGYNFQ---------SEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++ ++  HNK R  V        P A+NM  + W  E E  A  +  +C ++H   G +       +GQN+A +W         ++ WYDEVK Y +           E+CS   C HYTQ+VWA T   GC V  CS+  +        I+L CNY+P GNW  E  Y  G+ CS+CP   G  C D LC
Sbjct:   61 REEILALHNKLREGV-------YPTASNMEYMVWDDELERSATHWAEQCQWEH---GPQDL--LMSIGQNLAVHWGRYRSPAYHVQAWYDEVKDYTYPYPHECNPWCPERCSGPMCTHYTQLVWATTSRVGCAVHTCSRMNVWGEIWENAIYLVCNYSPKGNWIGEAPYAHGRPCSQCPPSYGGGCRDNLC 239          
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000374.1 (pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 gene:ENSPMAG00000000334.1 transcript:ENSPMAT00000000374.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.375 bits (259), Expect = 9.745e-27
Identity = 60/196 (30.61%), Postives = 93/196 (47.45%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDD--VTEGIKMWYDEVKGYNFQSEK---------CSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL------GKIFLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            +  D+++AL + HN+ R  V        P+AA+M  + W  E    A+ +  +C + H  S +        +GQN+A +   +  +   ++ W+DE + Y +  EK         C    C HYTQ+VWA+++  GC V  C    +        ++L CNY+P GNW  E  Y  G  CS+CP   G  C  GLC
Sbjct:    1 IADDERRALTEYHNRLRASV-------HPQAADMEHMSWDWELAGMAEAWAKQCKWGHGPSDQ-----LDAIGQNLAVYVGSERPLAYHVQAWFDEEEWYQYPEEKECSPECPHTCRGPICTHYTQLVWAESRRVGCAVHTCLDISVWDAVWPYALYLVCNYSPKGNWIGEAPYRQGTPCSRCPPRYGGSCRGGLC 184          
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: CRISP3 (cysteine rich secretory protein 3 [Source:HGNC Symbol;Acc:HGNC:16904])

HSP 1 Score: 97.4413 bits (241), Expect = 4.663e-25
Identity = 63/177 (35.59%), Postives = 86/177 (48.59%), Query Frame = 2
Query:  104 LIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWV-GQNIAYWHSDDVT--EGIKMWYDEVKGYNFQSEKCSVST--CLHYTQVVWAKTKEFGCGVADCSKKK-LGKIFLACNYAPGGNWNNE--KAYEVGKACSKCPTGTKCNDGLC 610
            ++D HN  RR V        P A NML++ W  EA   A  + N C F H     RK  + QW  G+NI +  S   T    I+ WY+EV    FQ +  S +T    HYTQ+VW  + + GC V  C K   + K    C+Y P GN N    + Y++G +C+ CP    C + LC
Sbjct:   47 IVDLHNDLRRNVT-------PSAGNMLQMMWKKEAYLNALSWANNCTFGHSPDARRK--TTQWTCGENI-FMSSAPFTWNYAIRDWYNEVISPGFQYDVGSTTTGAVGHYTQLVWYNSFQLGCAVNYCPKNPGVAKYLYVCHYCPMGNLNTRINRPYDLGTSCASCP--KNCVNNLC 211          
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002250.1 (pep scaffold:Pmarinus_7.0:GL477299:1626:22672:-1 gene:ENSPMAG00000002049.1 transcript:ENSPMAT00000002250.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.8189 bits (229), Expect = 5.424e-24
Identity = 57/185 (30.81%), Postives = 85/185 (45.95%), Query Frame = 2
Query:  110 DAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKF-GSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSE---------KCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL-GKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            + HNK R EV        P ++NM  + W  +   +A ++  +C+          F G   W+      +H       ++ WYDE K Y +  E         +C+ + C HYTQ+VWA T   GC +  C    + G+I     +L CNY+P GNW     Y+ G+ACS+CP   G  C + LC
Sbjct:    1 ELHNKLRGEV-------HPPSSNMEYMAWVKKLIKEAARYPGRCLRLMQPMAMIHFLGQPHWLSHRPPSFH-------VQAWYDEAKDYQYPYEQECNPWCPFRCNGAVCTHYTQLVWATTNRVGCAINTCYNMNVWGQIWEKAVYLVCNYSPKGNWIGLSPYKSGRACSECPPSYGGSCRNNLC 171          
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: CRISP2 (cysteine rich secretory protein 2 [Source:HGNC Symbol;Acc:HGNC:12024])

HSP 1 Score: 63.5438 bits (153), Expect = 7.924e-13
Identity = 35/97 (36.08%), Postives = 52/97 (53.61%), Query Frame = 2
Query:  335 EGIKMWYDEV--KGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNE--KAYEVGKACSKCPTGTKCNDGLCS 613
            E IK+W DE    G+++     +     HYTQ++W  T + GC +  C ++    ++L C Y P GNWNN   K Y+ G +C  CP    C+D LC+
Sbjct:   62 EAIKVWVDEANSPGFDYGKGPRNPGIVGHYTQIIWYSTYQVGCTINYCPEEAYKYMYL-CAYCPVGNWNNRLTKPYDEGPSCQDCP--NDCDDKLCT 155          
BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Match: CRISP2 (cysteine rich secretory protein 2 [Source:HGNC Symbol;Acc:HGNC:12024])

HSP 1 Score: 54.299 bits (129), Expect = 9.684e-10
Identity = 35/102 (34.31%), Postives = 47/102 (46.08%), Query Frame = 2
Query:  335 EGIKMWYDE--VKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNE--KAYEVGKACSKCPTGTKCNDGLCS*SKIY 628
            + +  WY+E  V G+ F     +     HYTQVVW  + + GC V  C  + + K    C+Y P GN N    + Y  G  C  CP    C + LCS S  Y
Sbjct:   16 DAVLDWYNEAEVPGFIFGKGPAAPGDIGHYTQVVWYSSFQLGCAVNYC-PEAIYKYVYVCHYCPAGNLNTRLSRPYNEGSPCRSCP--NDCENDLCSESCPY 114          
BLAST of Venom allergen 5 vs. Ensembl Yeast
Match: PRY3 (Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p [Source:SGD;Acc:S000003614])

HSP 1 Score: 67.781 bits (164), Expect = 8.992e-14
Identity = 51/159 (32.08%), Postives = 74/159 (46.54%), Query Frame = 2
Query:   86 PDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSG--ERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEV 556
            P+ +  +++ HNK+R       V   P       L WS      AQ + ++    +D SG      G Y   G+N+A  ++D  T  +  WY E+  YN+ +   S ST  H+TQVVW  T E GCG   C        ++ C+Y P GN+  E A EV
Sbjct:   23 PNFESDVLNEHNKFRAL----HVDTAP-------LTWSDTLATYAQNYADQ----YDCSGVLTHSDGPY---GENLALGYTD--TGAVDAWYGEISKYNYSNPGFSESTG-HFTQVVWKSTAEIGCGYKYCGTT--WNNYIVCSYNPPGNYLGEFAEEV 158          
BLAST of Venom allergen 5 vs. Ensembl Yeast
Match: PRY1 (Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication [Source:SGD;Acc:S000003615])

HSP 1 Score: 58.151 bits (139), Expect = 9.866e-11
Identity = 38/123 (30.89%), Postives = 59/123 (47.97%), Query Frame = 2
Query:  188 LKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEV 556
            L WS    + AQ + +     +D SG        + G+N+A  +  D    +  WY+E+  Y+F +   S +T  H+TQVVW  T + GCG+  C        ++ C+Y P GN+  E A  V
Sbjct:  183 LSWSDTLASYAQDYADN----YDCSGTLTHSGGPY-GENLALGY--DGPAAVDAWYNEISNYDFSNPGFSSNTG-HFTQVVWKSTTQVGCGIKTCGGAW--GDYVICSYDPAGNYEGEYADNV 295          
BLAST of Venom allergen 5 vs. Ensembl Yeast
Match: PRY2 (Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication [Source:SGD;Acc:S000001721])

HSP 1 Score: 49.2914 bits (116), Expect = 1.319e-7
Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = 2
Query:  188 LKWSSEAEAKAQKFVNKCVFKHDSSGE--RKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEV 556
            L WS      AQ + +     +D SG      G Y   G+N+A  +    T  +  WY+E+  Y++ +   S S   H+TQVVW  T E GCG+  C   + G  ++ C+Y   GN   E A  V
Sbjct:  213 LTWSDTLATYAQNYADS----YDCSGNLVHSGGPY---GENLALGYG--TTGSVDAWYNEITSYDYSNPGFSESAG-HFTQVVWKGTSEVGCGLKSCG-GEWGD-YIICSYKAAGNVIGEFADNV 325          
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO31682 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX85])

HSP 1 Score: 67.781 bits (164), Expect = 1.184e-14
Identity = 48/149 (32.21%), Postives = 70/149 (46.98%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEG----IKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGN 529
            +K  +DAHN++R     GK            L WS E  ++AQK+ N       S  + +       G+NI Y +  +  E     ++ WY EVK Y+F++     ST  H++Q+VW  TK+ G G A   + K G  FL   Y P GN
Sbjct:    4 QKQCLDAHNEFRTRHGAGK------------LAWSGELASEAQKWANHLA----SIDQMERDPLTNGGENIFYMYGGNPREACERAVRNWYQEVKNYDFKNPHSDPSTS-HFSQLVWKGTKKLGVGEA---QSKSGNFFLVARYHPKGN 132          
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO40545 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6Z3])

HSP 1 Score: 66.2402 bits (160), Expect = 9.233e-14
Identity = 48/155 (30.97%), Postives = 76/155 (49.03%), Query Frame = 2
Query:   92 QKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-----YWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNE 541
            +++AL+  HN +R      +V   P     L  + S +A+  A+K  N    +H SSGER     + VG+N+A     +       E +  WY+EV  Y+F +   ++ T  H+TQVVW  + E G G A      +  +F+   Y P GN+NN+
Sbjct:    7 EEEALL-THNNFR------QVHNAPPMT--LNRELSLQAKGYAEKIANLGALQHSSSGERG----EDVGENLAVGCKSFGVPLTGKEAVTNWYNEVCKYDFNNGAFAMETG-HFTQVVWTDSIELGMGKATGKLNGIPCVFVVGRYRPPGNFNNQ 147          
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO49310 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH92])

HSP 1 Score: 67.3958 bits (163), Expect = 2.305e-13
Identity = 54/150 (36.00%), Postives = 68/150 (45.33%), Query Frame = 2
Query:  107 IDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCV----FKHDSS----GERKFGSYQWVGQNIAYWHSDDVTEGI-KMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGN 529
            +DAHN+YR               N+  L WS+    +AQK+ NK        HD S    GE  F S    G N      DD  E   + WY EV+ YNFQ       T  H+TQVVW  ++E G   A   K K G +F+   Y+PGGN
Sbjct:    9 LDAHNRYR------------AMHNVPPLTWSNSIAREAQKWANKLAKEGKLIHDKSRSGQGENVFMS---SGANF-----DDAGEAACESWYQEVERYNFQRGGHQSGTG-HFTQVVWKSSEELGVARA---KSKKGAVFVVARYSPGGN 134          

HSP 2 Score: 65.4698 bits (158), Expect = 1.036e-12
Identity = 44/161 (27.33%), Postives = 74/161 (45.96%), Query Frame = 2
Query:   62 TICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSS----EAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGN 529
            T+    V  D +  ++D HN YR               N   L+WS+    +A+A A K   +   +H S  +R +      G+NI    H  D+ + +++WY+E + Y + +   +++T  H+TQ+VW  T+E G G A     +L   +    Y P GN
Sbjct:  203 TLGALPVDEDTRSRILDIHNNYR------------AMHNAAPLRWSAALAKDAQAWAGKLAREGRLEHASREDRYYK-----GENICRMSHHFDIGDALQIWYNESESYQYDNPGFALTTG-HFTQIVWRGTREVGVGFAKSPDGRL--TYAVARYNPPGN 343          
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO48881 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHM8])

HSP 1 Score: 62.7734 bits (151), Expect = 8.637e-13
Identity = 48/151 (31.79%), Postives = 67/151 (44.37%), Query Frame = 2
Query:   95 KKALIDAHNKYRREVAQGKVSKQPEAANMLELKW----SSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVT--EGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGN 529
            K  ++  HN YR +                 LKW    +SEA++ A+    +   +H SS E         G++IAY     +T  +   MWY EV  Y F++   S S+  H+TQVVWA + E G G A  S    G  F+   Y P GN
Sbjct:    3 KNDILKTHNDYRAQ------------HGTKALKWNARLASEAQSWAENLAQRNAIQHSSSRE--------YGESIAYMSGAVLTGRKATDMWYGEVDKYRFENPGFSTSSG-HFTQVVWAGSTEMGAGKATSSS---GAHFVVARYTPPGN 129          
BLAST of Venom allergen 5 vs. Ensembl Nematostella
Match: EDO35287 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLZ5])

HSP 1 Score: 64.3142 bits (155), Expect = 4.892e-12
Identity = 47/158 (29.75%), Postives = 74/158 (46.84%), Query Frame = 2
Query:   68 CHCNVTPDQ-KKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIA-YWHSDDVT--EGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGN 529
            C+    P++  KA +  HN++RR        K P       LKWS +   KAQK         + + +  +   +  G+NIA + H  D+      + WY+EV+GY F S     +T  H++Q+VW  TK  G GVA  ++    + ++   Y P GN
Sbjct:  692 CYQGPCPEEIAKAGLKLHNQFRRW------HKTPS------LKWSDKLAKKAQKLAQSMAADSNHTDDEAYSQNELPGENIAVFKHDYDIAAERATRKWYEEVEGYRFGSPVLGEAT-KHFSQLVWKGTKSVGIGVARGARD---RTYVVALYDPPGN 833          
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000020676.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728])

HSP 1 Score: 103.219 bits (256), Expect = 9.045e-27
Identity = 63/178 (35.39%), Postives = 89/178 (50.00%), Query Frame = 2
Query:  104 LIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGI-KMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFL-ACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS*SKIYI 631
            ++D HN +RR V       +P AA+ML + +S E    AQ +V+KCV  H     R    Y+ +G+N+ Y  S  + + I   WY EV  + F +   +     HYTQVVW  + + GCGV  CS      I+L  C+Y   GN+     Y+VG  C+ CP    C D LC+    YI
Sbjct:   19 IVDVHNAFRRAV-------EPPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYE-LGENLFYSTSPMLWKSIINAWYKEVSHFAFPNASTNGKPVGHYTQVVWNSSYKVGCGVTLCSN----NIYLYGCHYYRAGNFKRWPPYKVGTPCASCP--NDCVDKLCTNPCPYI 182          
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000020674.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728])

HSP 1 Score: 102.834 bits (255), Expect = 1.364e-26
Identity = 63/178 (35.39%), Postives = 89/178 (50.00%), Query Frame = 2
Query:  104 LIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGI-KMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFL-ACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS*SKIYI 631
            ++D HN +RR V       +P AA+ML + +S E    AQ +V+KCV  H     R    Y+ +G+N+ Y  S  + + I   WY EV  + F +   +     HYTQVVW  + + GCGV  CS      I+L  C+Y   GN+     Y+VG  C+ CP    C D LC+    YI
Sbjct:   34 IVDVHNAFRRAV-------EPPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYE-LGENLFYSTSPMLWKSIINAWYKEVSHFAFPNASTNGKPVGHYTQVVWNSSYKVGCGVTLCSN----NIYLYGCHYYRAGNFKRWPPYKVGTPCASCP--NDCVDKLCTNPCPYI 197          
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: pi15a (peptidase inhibitor 15 [Source:NCBI gene;Acc:101173911])

HSP 1 Score: 103.219 bits (256), Expect = 1.779e-26
Identity = 63/196 (32.14%), Postives = 97/196 (49.49%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYW--HSDDVTEGIKMWYDEVKGYNFQSEK---------CSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL-GKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            ++ +   A++D HNK R     GKV   P A+NM  + W       A+ + + C+++H  S   +F     +GQN++        + + +K WYDEVK Y+F   +         C    C HYTQ+VWA + + GC +  C    + G +     +L CNY+P GNW  E  Y+VG  CS CP   G  C++ +C
Sbjct:   61 ISQNDMLAILDYHNKVR-----GKVF--PPASNMEYMVWDETLAKTAEDWAHACLWEHGPSHLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYSFPYPRDCNPRCPLRCYGPMCTHYTQMVWATSNKVGCAIHTCYNMNVWGSVWKRATYLVCNYSPKGNWIGEAPYKVGVPCSACPPSYGGSCSNNMC 244          
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 106.686 bits (265), Expect = 2.248e-26
Identity = 65/196 (33.16%), Postives = 95/196 (48.47%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYW-HSDDVTEGIKMWYDEVKGYNFQSE---------KCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL-GKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            +T    + ++D HNK R +V        P A+NM  + W +E E  A+++   C+++H  +          +GQN+ A+W      T  ++ WYDEVK Y+F            +CS   C HYTQ+VWA +   GC +  C    + G+I     +L CNY+P GNW     Y+ G  CS CP   G  C D LC
Sbjct:   84 ITDSDAQLILDLHNKLRGQV-------YPPASNMEYMMWDTELERSAEEWAETCLWEHGPAS-----LLPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSFPYPQECNPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMDVWGQIWAKAVYLVCNYSPKGNWWGHAPYKHGTPCSACPPSYGGGCRDNLC 267          
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 106.686 bits (265), Expect = 2.309e-26
Identity = 65/196 (33.16%), Postives = 95/196 (48.47%), Query Frame = 2
Query:   80 VTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNI-AYW-HSDDVTEGIKMWYDEVKGYNFQSE---------KCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKL-GKI-----FLACNYAPGGNWNNEKAYEVGKACSKCPT--GTKCNDGLC 610
            +T    + ++D HNK R +V        P A+NM  + W +E E  A+++   C+++H  +          +GQN+ A+W      T  ++ WYDEVK Y+F            +CS   C HYTQ+VWA +   GC +  C    + G+I     +L CNY+P GNW     Y+ G  CS CP   G  C D LC
Sbjct:   56 ITDSDAQLILDLHNKLRGQV-------YPPASNMEYMMWDTELERSAEEWAETCLWEHGPAS-----LLPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSFPYPQECNPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMDVWGQIWAKAVYLVCNYSPKGNWWGHAPYKHGTPCSACPPSYGGGCRDNLC 239          
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000062601.1 (SMESG000062601.1)

HSP 1 Score: 411.764 bits (1057), Expect = 1.402e-148
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 2
Query:   20 MKMNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 613
            MKMNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS
Sbjct:    1 MKMNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 198          
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000062601.1 (SMESG000062601.1)

HSP 1 Score: 407.527 bits (1046), Expect = 6.999e-147
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 2
Query:   26 MNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 613
            MNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS
Sbjct:    1 MNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 196          
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000062609.1 (SMESG000062609.1)

HSP 1 Score: 299.671 bits (766), Expect = 2.745e-104
Identity = 133/191 (69.63%), Postives = 158/191 (82.72%), Query Frame = 2
Query:   41 VFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 613
            + + + +++  C VTP Q+K LIDAHNKYRR+ AQGK+  QP AANMLELKW++EA  +AQ   NKCVF HD   +RKFG+Y WVGQNIA+W SDDVTEG+KMWYDEV GYNF S++CS  TCLHYT +VWAKTKEFGCG+ADCS++ LGKIFL CNY PGGNWN EK YEVGKACSKCP+GT+CN+GLCS
Sbjct:    9 LIICVVLSVIECGVTPAQQKQLIDAHNKYRRDAAQGKIGGQPAAANMLELKWNNEAAQRAQNSANKCVFGHDQPRDRKFGNYDWVGQNIAFWSSDDVTEGVKMWYDEVNGYNFNSDQCSGFTCLHYTALVWAKTKEFGCGIADCSRQNLGKIFLFCNYGPGGNWNREKPYEVGKACSKCPSGTRCNNGLCS 199          
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000047165.1 (SMESG000047165.1)

HSP 1 Score: 248.44 bits (633), Expect = 5.327e-84
Identity = 107/160 (66.88%), Postives = 133/160 (83.12%), Query Frame = 2
Query:  134 EVAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 613
            +VAQG++S QP AANMLELKW+ EA  +AQ   ++CVF+HD S +R+FG Y WVGQNIAYW S+DVT+GIKMW+DEVK Y F  +KCS  TCLHYT ++WAKTKEFGCG+ADCS+K LG I++ CNY PGGNWNNE  Y+VG+ACS+CP GT+C++GLCS
Sbjct:   46 DVAQGQISGQPAAANMLELKWNDEAAQRAQMSADRCVFQHDQSRDRRFGGYSWVGQNIAYWSSNDVTDGIKMWFDEVKDYTFNGDKCSGFTCLHYTALIWAKTKEFGCGIADCSRKGLGNIYIVCNYGPGGNWNNEAPYKVGQACSRCPNGTRCSNGLCS 205          
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000047165.1 (SMESG000047165.1)

HSP 1 Score: 246.128 bits (627), Expect = 9.703e-84
Identity = 107/159 (67.30%), Postives = 132/159 (83.02%), Query Frame = 2
Query:  137 VAQGKVSKQPEAANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWHSDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVADCSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS 613
            VAQG++S QP AANMLELKW+ EA  +AQ   ++CVF+HD S +R+FG Y WVGQNIAYW S+DVT+GIKMW+DEVK Y F  +KCS  TCLHYT ++WAKTKEFGCG+ADCS+K LG I++ CNY PGGNWNNE  Y+VG+ACS+CP GT+C++GLCS
Sbjct:    1 VAQGQISGQPAAANMLELKWNDEAAQRAQMSADRCVFQHDQSRDRRFGGYSWVGQNIAYWSSNDVTDGIKMWFDEVKDYTFNGDKCSGFTCLHYTALIWAKTKEFGCGIADCSRKGLGNIYIVCNYGPGGNWNNEAPYKVGQACSRCPNGTRCSNGLCS 159          
The following BLAST results are available for this feature:
BLAST of Venom allergen 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PI161.510e-2834.05peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI161.510e-2834.05peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI161.510e-2834.05peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI154.410e-2833.16peptidase inhibitor 15 [Source:HGNC Symbol;Acc:HGN... [more]
PI154.410e-2833.16peptidase inhibitor 15 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Venom allergen 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scl-51.289e-3031.48SCP-Like extracellular protein [Source:UniProtKB/... [more]
scl-22.896e-2831.63SCP-Like extracellular protein [Source:UniProtKB/... [more]
scl-22.896e-2831.63SCP-Like extracellular protein [Source:UniProtKB/... [more]
scl-101.511e-2730.14SCP-Like extracellular protein [Source:UniProtKB/... [more]
scl-142.462e-2530.05SCP-Like extracellular protein [Source:UniProtKB/... [more]
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BLAST of Venom allergen 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG84836.127e-2636.31gene:FBgn0038126 transcript:FBtr0082782[more]
Ag5r4.120e-1630.48gene:FBgn0015010 transcript:FBtr0333658[more]
Ag5r4.120e-1630.48gene:FBgn0015010 transcript:FBtr0073937[more]
Ag5r4.120e-1630.48gene:FBgn0015010 transcript:FBtr0305582[more]
CG326794.221e-1429.41gene:FBgn0052679 transcript:FBtr0071469[more]
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BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
r3hdml1.914e-2733.69R3H domain containing-like [Source:ZFIN;Acc:ZDB-GE... [more]
r3hdml1.914e-2733.69R3H domain containing-like [Source:ZFIN;Acc:ZDB-GE... [more]
crispld21.547e-2634.03cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a1.861e-2633.16cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a2.100e-2633.16cysteine-rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crispld21.877e-2733.51cysteine-rich secretory protein LCCL domain contai... [more]
crispld22.070e-2733.51cysteine-rich secretory protein LCCL domain contai... [more]
crispld22.317e-2733.51cysteine-rich secretory protein LCCL domain contai... [more]
CRISPLD11.310e-2633.16cysteine-rich secretory protein LCCL domain contai... [more]
ENSXETT00000036788.11.592e-2634.25pep primary_assembly:Xenopus_tropicalis_v9.1:KV464... [more]
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BLAST of Venom allergen 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pi161.080e-3134.78peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi161.644e-3134.78peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi161.644e-3134.78peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi163.871e-3035.14peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi155.170e-2833.16peptidase inhibitor 15 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of Venom allergen 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0DPU1|VA576_SCODE1.630e-3438.33Scoloptoxin SSD976 OS=Scolopendra dehaani OX=26097... [more]
sp|P0DPU0|VA552_SCODE1.894e-3438.80Scoloptoxin SSD552 OS=Scolopendra dehaani OX=26097... [more]
sp|P0DPV2|VA558_SCODE1.711e-3337.78Scoloptoxin SSD558 OS=Scolopendra dehaani OX=26097... [more]
sp|Q7Z156|VA5_POLSR2.276e-2939.77Venom allergen 5 OS=Polybia scutellaris rioplatens... [more]
sp|Q9ET66|PI16_MOUSE2.708e-2935.14Peptidase inhibitor 16 OS=Mus musculus OX=10090 GN... [more]
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BLAST of Venom allergen 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A564YFQ73.284e-4748.68SCP domain-containing protein OS=Hymenolepis dimin... [more]
A0A1C9ZZV53.700e-4640.30SCP domain-containing protein (Fragment) OS=Dugesi... [more]
A0A5K3ENG84.525e-4544.94Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A564Z3X22.479e-4248.03SCP domain-containing protein OS=Hymenolepis dimin... [more]
A0A0R3SFR13.573e-4247.55SCP domain-containing protein OS=Hymenolepis dimin... [more]
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BLAST of Venom allergen 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000036030.13.015e-2933.85peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000031557.13.976e-2935.00peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000029824.15.515e-2935.00peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
r3hdml1.896e-2733.51R3H domain containing like [Source:NCBI gene;Acc:1... [more]
crispld21.168e-2634.55cysteine-rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000374.19.745e-2730.61pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 ... [more]
CRISP34.663e-2535.59cysteine rich secretory protein 3 [Source:HGNC Sym... [more]
ENSPMAT00000002250.15.424e-2430.81pep scaffold:Pmarinus_7.0:GL477299:1626:22672:-1 g... [more]
CRISP27.924e-1336.08cysteine rich secretory protein 2 [Source:HGNC Sym... [more]
CRISP29.684e-1034.31cysteine rich secretory protein 2 [Source:HGNC Sym... [more]
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BLAST of Venom allergen 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
PRY38.992e-1432.08Cell wall-associated protein involved in export of... [more]
PRY19.866e-1130.89Sterol binding protein involved in the export of a... [more]
PRY21.319e-732.00Sterol binding protein involved in the export of a... [more]
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BLAST of Venom allergen 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO316821.184e-1432.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO405459.233e-1430.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO493102.305e-1336.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO488818.637e-1331.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO352874.892e-1229.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Venom allergen 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000020676.29.045e-2735.39cysteine-rich venom protein pseudecin-like [Source... [more]
ENSORLT00000020674.21.364e-2635.39cysteine-rich venom protein pseudecin-like [Source... [more]
pi15a1.779e-2632.14peptidase inhibitor 15 [Source:NCBI gene;Acc:10117... [more]
crispld1a2.248e-2633.16cysteine rich secretory protein LCCL domain contai... [more]
crispld1a2.309e-2633.16cysteine rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062601.11.402e-148100.00SMESG000062601.1[more]
SMESG000062601.16.999e-147100.00SMESG000062601.1[more]
SMESG000062609.12.745e-10469.63SMESG000062609.1[more]
SMESG000047165.15.327e-8466.88SMESG000047165.1[more]
SMESG000047165.19.703e-8467.30SMESG000047165.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30008009 ID=SMED30008009|Name=Venom allergen 5|organism=Schmidtea mediterranea sexual|type=transcript|length=667bp
ATCTTAAAGAATAAATAAAATGAAAATGAATTTTAAATTGGTATTTGTAG
CGATCTATATAACAATTTGCCACTGTAACGTGACCCCCGATCAGAAAAAG
GCTTTGATTGATGCTCACAACAAATACCGCCGAGAAGTAGCTCAAGGAAA
AGTTAGTAAACAACCCGAAGCTGCAAACATGCTAGAATTGAAATGGAGTA
GCGAAGCAGAGGCAAAGGCGCAGAAGTTCGTAAATAAATGTGTATTCAAA
CACGATTCTTCGGGTGAAAGAAAATTTGGAAGTTACCAATGGGTTGGGCA
AAATATCGCATATTGGCACAGTGATGATGTTACAGAAGGTATTAAAATGT
GGTATGATGAAGTGAAGGGTTACAATTTCCAATCCGAAAAATGCTCCGTC
TCAACATGCTTGCATTATACTCAAGTAGTATGGGCAAAAACGAAAGAGTT
TGGATGCGGTGTTGCTGACTGTTCAAAGAAAAAACTAGGAAAAATATTTT
TAGCTTGTAATTATGCACCTGGTGGAAATTGGAATAATGAAAAGGCCTAT
GAAGTTGGAAAAGCATGCAGTAAATGCCCAACCGGGACCAAATGCAATGA
TGGACTATGCAGTTAATCTAAAATTTATATTTTAATCAAAAGAATTGAAA
CTGAAATACTTTTAAAA
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protein sequence of SMED30008009-orf-1

>SMED30008009-orf-1 ID=SMED30008009-orf-1|Name=SMED30008009-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=199bp
MKMNFKLVFVAIYITICHCNVTPDQKKALIDAHNKYRREVAQGKVSKQPE
AANMLELKWSSEAEAKAQKFVNKCVFKHDSSGERKFGSYQWVGQNIAYWH
SDDVTEGIKMWYDEVKGYNFQSEKCSVSTCLHYTQVVWAKTKEFGCGVAD
CSKKKLGKIFLACNYAPGGNWNNEKAYEVGKACSKCPTGTKCNDGLCS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR035940CAP_sf
IPR001283CRISP-related
IPR002413V5_allergen-like
IPR014044CAP_domain
IPR018244Allrgn_V5/Tpx1_CS
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001283Cysteine-rich secretory protein-relatedPRINTSPR00837V5TPXLIKEcoord: 57..75
score: 30.97
coord: 130..146
score: 57.65
coord: 160..173
score: 46.17
coord: 107..120
score: 34.36
IPR001283Cysteine-rich secretory protein-relatedPANTHERPTHR10334CYSTEINE-RICH SECRETORY PROTEIN-RELATEDcoord: 20..194
IPR002413Venom allergen 5-likePRINTSPR00838V5ALLERGENcoord: 105..120
score: 48.83
coord: 129..148
score: 65.31
coord: 20..36
score: 26.1
IPR014044CAP domainSMARTSM00198SCP_3coord: 24..173
e-value: 1.6E-52
score: 190.4
IPR014044CAP domainPFAMPF00188CAPcoord: 31..165
e-value: 1.2E-23
score: 84.3
IPR035940CAP superfamilyGENE3DG3DSA:3.40.33.10coord: 12..198
e-value: 1.4E-57
score: 196.5
IPR035940CAP superfamilySUPERFAMILYSSF55797PR-1-likecoord: 8..180
NoneNo IPR availablePANTHERPTHR10334:SF344AT27079P-RELATEDcoord: 20..194
NoneNo IPR availableCDDcd05380CAP_eukcoord: 26..166
e-value: 1.5716E-44
score: 142.979
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.695
IPR018244Allergen V5/Tpx-1-related, conserved sitePROSITEPS01010CRISP_2coord: 160..171