Protein Tob1

Overview
NameProtein Tob1
Smed IDSMED30007982
Length (bp)1562
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein Tob1 (SMED30007982) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein Tob1 (SMED30007982) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein Tob1 (SMED30007982) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein Tob1 (SMED30007982) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007982

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30007982h1SMcG0003753 dd_Smed_v4_13216_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30007982h1SMcG0003753 dd_Smed_v4_13216_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Protein Tob1 vs. Ensembl Human
Match: TOB2 (transducer of ERBB2, 2 [Source:HGNC Symbol;Acc:HGNC:11980])

HSP 1 Score: 83.1889 bits (204), Expect = 9.091e-17
Identity = 64/220 (29.09%), Postives = 102/220 (46.36%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLK 868
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W  +     +  + S     +IG     V+ ++   A    + + ++ +  PE L ++I+P ++ Y I            ++   ++  L       +PE +++ +            I  + +      S    Q+PS             FI +S+    FT ASFA TKFGSTK+K
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLKKKYEGHWYPE-----KPLKGSGFRCVHIG---EMVDPVVELAAKRSGLAVEDVRANVPEELSVWIDPFEVSYQI-----------GEKGAVKVLYLDDSEGCGAPELDKEIKSSFNPDAQVFVPIGSQDSSLSNSPSPSFGQSPSP-----------TFIPRSAQPITFTTASFAATKFGSTKMK 192
[Close]
BLAST of Protein Tob1 vs. Ensembl Human
Match: TOB1 (transducer of ERBB2, 1 [Source:HGNC Symbol;Acc:HGNC:11979])

HSP 1 Score: 79.7221 bits (195), Expect = 1.231e-15
Identity = 42/109 (38.53%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL+   E  W  +     + Y+ S     +IG    +V+ +I Q +    +DI ++    P++L ++I+P ++ Y I
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPE-----KPYKGSGFRCIHIG---EKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Human
Match: TOB1 (transducer of ERBB2, 1 [Source:HGNC Symbol;Acc:HGNC:11979])

HSP 1 Score: 79.7221 bits (195), Expect = 1.231e-15
Identity = 42/109 (38.53%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL+   E  W  +     + Y+ S     +IG    +V+ +I Q +    +DI ++    P++L ++I+P ++ Y I
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPE-----KPYKGSGFRCIHIG---EKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Human
Match: TOB2 (transducer of ERBB2, 2 [Source:HGNC Symbol;Acc:HGNC:11980])

HSP 1 Score: 68.1662 bits (165), Expect = 1.609e-13
Identity = 38/107 (35.51%), Postives = 61/107 (57.01%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQY 529
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W  +     +  + S     +IG     V+ ++   A    + + ++ +  PE L ++I+P ++ Y
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLKKKYEGHWYPE-----KPLKGSGFRCVHIG---EMVDPVVELAAKRSGLAVEDVRANVPEELSVWIDPFEVSY 101
[Close]
BLAST of Protein Tob1 vs. Ensembl Celegans
Match: fog-3 (FOG-3 protein; Feminization Of Germline [Source:UniProtKB/TrEMBL;Acc:G5EDX7])

HSP 1 Score: 56.6102 bits (135), Expect = 9.296e-9
Identity = 53/238 (22.27%), Postives = 91/238 (38.24%), Query Frame = 2 
Query:  227 VNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYE--------ILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYER----------KSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEG--CFIQKSSSTPNFTAASFAQTKFGSTKLKSQLK 880
            VNF+  YL+  +PRR V +FG  L   L S+    W     K+                   ++  MIN   + C     E        +L   P N+I+F  PG +   +        IW  D    E ++ + E ++++               KS    ++ + + +K  L    T+ YS     +   +   +  +       F+ K SS   F+   F+QT+FGS+K +  L+
Sbjct:    9 VNFVCRYLFGHIPRRPVGIFGAELGNYLVSHFSSTWDVHHPKN------------------GEMKRMINTTTSLCFASSAEEAGVPPSDVLRLLPTNMIIFANPGHVFVRLSENGIETPIWIGDVNCDENYQSVPEYVVRTAAIRAEPCSNLGAAGKSVLVGKKPLLTNDKAALEMVNTM-YSPLAREKCDDINANLSHLREMYPFRFVYKPSSAQTFSGVEFSQTRFGSSKSRPDLQ 227
[Close]
BLAST of Protein Tob1 vs. Ensembl Fly
Match: Tob (gene:FBgn0028397 transcript:FBtr0074255)

HSP 1 Score: 74.3294 bits (181), Expect = 8.666e-14
Identity = 37/109 (33.94%), Postives = 64/109 (58.72%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NF++SYLYNKLPRRRV++FGE LE+ L+   +  W  +     + ++ S       G   + ++S++ + A    + I +IL   P  L ++I+PG++ + I
Sbjct:    3 IEIQVALNFVISYLYNKLPRRRVNIFGEELEKALRDKFQDHWYPE-----KPFKGSAYRCLKTG---DPIDSVLERAARESGVPIGDILENLPNELSVWIDPGEVSFRI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Fly
Match: Tob (gene:FBgn0028397 transcript:FBtr0310446)

HSP 1 Score: 74.3294 bits (181), Expect = 8.666e-14
Identity = 37/109 (33.94%), Postives = 64/109 (58.72%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NF++SYLYNKLPRRRV++FGE LE+ L+   +  W  +     + ++ S       G   + ++S++ + A    + I +IL   P  L ++I+PG++ + I
Sbjct:    3 IEIQVALNFVISYLYNKLPRRRVNIFGEELEKALRDKFQDHWYPE-----KPFKGSAYRCLKTG---DPIDSVLERAARESGVPIGDILENLPNELSVWIDPGEVSFRI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Fly
Match: Tob (gene:FBgn0028397 transcript:FBtr0301652)

HSP 1 Score: 73.9442 bits (180), Expect = 1.356e-13
Identity = 37/109 (33.94%), Postives = 64/109 (58.72%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NF++SYLYNKLPRRRV++FGE LE+ L+   +  W  +     + ++ S       G   + ++S++ + A    + I +IL   P  L ++I+PG++ + I
Sbjct:    3 IEIQVALNFVISYLYNKLPRRRVNIFGEELEKALRDKFQDHWYPE-----KPFKGSAYRCLKTG---DPIDSVLERAARESGVPIGDILENLPNELSVWIDPGEVSFRI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Fly
Match: Tob (gene:FBgn0028397 transcript:FBtr0310317)

HSP 1 Score: 73.559 bits (179), Expect = 1.420e-13
Identity = 37/109 (33.94%), Postives = 64/109 (58.72%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NF++SYLYNKLPRRRV++FGE LE+ L+   +  W  +     + ++ S       G   + ++S++ + A    + I +IL   P  L ++I+PG++ + I
Sbjct:    3 IEIQVALNFVISYLYNKLPRRRVNIFGEELEKALRDKFQDHWYPE-----KPFKGSAYRCLKTG---DPIDSVLERAARESGVPIGDILENLPNELSVWIDPGEVSFRI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Zebrafish
Match: tob1b (transducer of ERBB2, 1b [Source:ZFIN;Acc:ZDB-GENE-040426-2151])

HSP 1 Score: 95.5153 bits (236), Expect = 3.380e-21
Identity = 72/231 (31.17%), Postives = 118/231 (51.08%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQE-QELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+ L+   E  W       D+ Y+ S     ++G    +V+ ++ + A    +DI ++ +  P++L ++I+P ++ Y I   +    +   D       EL KE         R S + E Q  +  +E + + +  + P    F  Q+ SV P  +         Q+S+    FT ASFA TKFGSTK+K+  +   +++
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERQLKKKYEGHWYP-----DKPYKGSGFRCIHVG---EKVDPVVEEAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQIG-EKGAVKVLYIDDNSENGSELDKE--------IRNSFNPEAQVFMPISEPVGVSSESSSPSPPPFG-QSASVSPSFMP--------QRSAQPLTFTTASFAATKFGSTKMKNSGRNGGKVA 207
[Close]
BLAST of Protein Tob1 vs. Ensembl Zebrafish
Match: tob1a (transducer of ERBB2, 1a [Source:ZFIN;Acc:ZDB-GENE-031030-4])

HSP 1 Score: 80.8777 bits (198), Expect = 3.082e-16
Identity = 41/109 (37.61%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+ L+   E  W       D+ Y+ S     ++G    +V+ ++ Q A    +DI ++ +  P++L ++I+P ++ Y I
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERQLKQKYEGHWYP-----DKPYKGSGFRCIHVG---EKVDPVVEQAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Xenopus
Match: tob1 (transducer of ERBB2, 1 [Source:NCBI gene;Acc:549365])

HSP 1 Score: 90.5077 bits (223), Expect = 1.728e-19
Identity = 69/230 (30.00%), Postives = 113/230 (49.13%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL++  E  W       D+ Y+ S     ++G    +V  +I Q A    ++I +I    P++L ++I+P ++ Y I   +    +   D       EL K       E +     + Q  +   ++ +  ++   P  +  A  +P+  P++ Q  +             FT A FA TKFGSTK+K+   + +R S
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRVNIFGEELEKLLKNKYEGHWYP-----DRPYKGSGFRCIHVG---EKVEPIIQQAANESGLEIEDIRRNLPQDLSVWIDPSEVSYQIG-EKGQIKVLYVDDNSENGTELDK-------EIKNSFNPEAQAFMPINDQSSSVSSSPSPLFNETAAVSPTFMPRLNQPLT-------------FTTAMFAATKFGSTKMKNNRNKIARSS 203
[Close]
BLAST of Protein Tob1 vs. Ensembl Xenopus
Match: tob2 (transducer of ERBB2, 2 [Source:NCBI gene;Acc:548967])

HSP 1 Score: 80.4925 bits (197), Expect = 4.619e-16
Identity = 40/109 (36.70%), Postives = 61/109 (55.96%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W  +       Y   ++           V+ ++ Q A    +DI ++ +  PE L ++I+P ++ Y I
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLKGKYEGHWYPEKPLKGSGYRCVHI--------GETVDPVVEQAARRSGLDIDDVRANVPEELSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Mouse
Match: Tob2 (transducer of ERBB2, 2 [Source:MGI Symbol;Acc:MGI:1888525])

HSP 1 Score: 72.0182 bits (175), Expect = 3.239e-13
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W  +     +  + S     +IG     V+ ++   A    + + ++ +  PE L ++I+P ++ Y I
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLRKKYEGHWYPE-----KPLKGSGFRCVHIG---EVVDPVVELAAKRSGLAVEDVRANVPEELSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Mouse
Match: Tob2 (transducer of ERBB2, 2 [Source:MGI Symbol;Acc:MGI:1888525])

HSP 1 Score: 61.2326 bits (147), Expect = 7.576e-12
Identity = 27/42 (64.29%), Postives = 33/42 (78.57%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFW 334
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLRKKYEGHW 44
[Close]
BLAST of Protein Tob1 vs. Ensembl Mouse
Match: Tob1 (transducer of ErbB-2.1 [Source:MGI Symbol;Acc:MGI:1349721])

HSP 1 Score: 60.8474 bits (146), Expect = 1.356e-9
Identity = 67/230 (29.13%), Postives = 112/230 (48.70%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL+   E  W  +     + Y+ S     ++G    +V+ +I Q +    +DI ++    P++L ++         I P E ++ I      K    +   E   +  +  + S + E ++      I+   +      S     + +V P          F+ +S+    FT A+FA TKFGSTK+K+   RSS+++
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPE-----KPYKGSGFRCIHVG---EKVDPVIEQASKESGLDIDDVRGNLPQDLSVW---------IDPFEVSYQIGEKGPVKVLYVDDSSETGCELDKEIKNSFNPEAQVFMP---ISDPASSVSSSPSPPFGHSAAVSP---------TFMPRSTQPLTFTTATFAATKFGSTKMKNS-GRSSKVA 202
[Close]
BLAST of Protein Tob1 vs. UniProt/SwissProt
Match: sp|Q14106|TOB2_HUMAN (Protein Tob2 OS=Homo sapiens OX=9606 GN=TOB2 PE=1 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 4.366e-16
Identity = 64/220 (29.09%), Postives = 102/220 (46.36%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLK 868
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W  +     +  + S     +IG     V+ ++   A    + + ++ +  PE L ++I+P ++ Y I            ++   ++  L       +PE +++ +            I  + +      S    Q+PS             FI +S+    FT ASFA TKFGSTK+K
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLKKKYEGHWYPE-----KPLKGSGFRCVHIG---EMVDPVVELAAKRSGLAVEDVRANVPEELSVWIDPFEVSYQI-----------GEKGAVKVLYLDDSEGCGAPELDKEIKSSFNPDAQVFVPIGSQDSSLSNSPSPSFGQSPSP-----------TFIPRSAQPITFTTASFAATKFGSTKMK 192
[Close]
BLAST of Protein Tob1 vs. UniProt/SwissProt
Match: sp|P50616|TOB1_HUMAN (Protein Tob1 OS=Homo sapiens OX=9606 GN=TOB1 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.912e-15
Identity = 42/109 (38.53%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL+   E  W  +     + Y+ S     +IG    +V+ +I Q +    +DI ++    P++L ++I+P ++ Y I
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPE-----KPYKGSGFRCIHIG---EKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. UniProt/SwissProt
Match: sp|Q8R5K6|TOB1_RAT (Protein Tob1 OS=Rattus norvegicus OX=10116 GN=Tob1 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.179e-14
Identity = 41/109 (37.61%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL+   E  W  +     + Y+ S     ++G    +V+ +I Q +    +DI ++    P++L ++I+P ++ Y I
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKQKYEGHWYPE-----KPYKGSGFRCIHVG---EKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. UniProt/SwissProt
Match: sp|Q9JM55|TOB2_MOUSE (Protein Tob2 OS=Mus musculus OX=10090 GN=Tob2 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.266e-12
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE+LL+   E  W  +     +  + S     +IG     V+ ++   A    + + ++ +  PE L ++I+P ++ Y I
Sbjct:    3 LEIKVALNFIISYLYNKLPRRRADLFGEELERLLRKKYEGHWYPE-----KPLKGSGFRCVHIG---EVVDPVVELAAKRSGLAVEDVRANVPEELSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. UniProt/SwissProt
Match: sp|Q61471|TOB1_MOUSE (Protein Tob1 OS=Mus musculus OX=10090 GN=Tob1 PE=1 SV=2)

HSP 1 Score: 60.8474 bits (146), Expect = 9.484e-9
Identity = 67/230 (29.13%), Postives = 112/230 (48.70%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+LL+   E  W  +     + Y+ S     ++G    +V+ +I Q +    +DI ++    P++L ++         I P E ++ I      K    +   E   +  +  + S + E ++      I+   +      S     + +V P          F+ +S+    FT A+FA TKFGSTK+K+   RSS+++
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPE-----KPYKGSGFRCIHVG---EKVDPVIEQASKESGLDIDDVRGNLPQDLSVW---------IDPFEVSYQIGEKGPVKVLYVDDSSETGCELDKEIKNSFNPEAQVFMP---ISDPASSVSSSPSPPFGHSAAVSP---------TFMPRSTQPLTFTTATFAATKFGSTKMKNS-GRSSKVA 202
[Close]
BLAST of Protein Tob1 vs. TrEMBL
Match: R7TUR9 (Anti_prolifrtn domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_24957 PE=4 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 5.403e-25
Identity = 81/261 (31.03%), Postives = 123/261 (47.13%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTF--------------------AW------QTPSVGPQIVQ-----VSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E +VA+NF++SYLYNKLPRRRVDMFGE +E+ L+   E  W       DQ ++ S      I I  ++++ ++   A A  +DI E+  Y P  L ++I+P ++ Y                       L ++G+ K    +RK  D  +  + + +++   + G  P + +F                    AW       +PS  P  V       S    F+ +      FTAA+FAQTKFGSTKLK+Q KR SR+S
Sbjct:    3 VEVSVALNFVISYLYNKLPRRRVDMFGEEVEKGLKKKFEGHWYP-----DQPFKGSGFRCIRISI--DKMDYVLTNAAHASGLDIDEVKEYLPNELTVWIDPHEVSY----------------------RLGEKGVAKILYSDRKDDDGLESSLDADKEVQSVSRGFNPEAQSFKPIDSLASPQSNVSLSPSPNAWLQAASSTSPSHQPSPVNGAYASTSPVNSFVSRVPKQTTFTAATFAQTKFGSTKLKTQAKRPSRLS 234
[Close]
BLAST of Protein Tob1 vs. TrEMBL
Match: A0A433U0S2 (Anti_prolifrtn domain-containing protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_004851 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.804e-22
Identity = 76/249 (30.52%), Postives = 117/249 (46.99%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTF--------AWQTP---SVGPQIVQVSSEGCFIQKS-----SSTPN---FTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E +VA+NF++SYLYNKLPRRRVD+FGE LE  L+   +  W  +       Y         +    + ++ ++   A    +DI+E+ +Y PE+L ++I+P ++ Y I                       + G++K    E K            ++I   + G  P + TF        A   P   S  P  + VS     + ++     +  PN   FTAA FAQTKFGSTKLK+Q+KR +R+S
Sbjct:    3 VEISVALNFVISYLYNKLPRRRVDLFGEELEVGLKEKFQGHWYPERPSKGSGYRC-------VKGNGDGIDPVLVDAATKAGLDIFEVKTYLPEDLTIWIDPSEVSYKIG----------------------ERGVVKILYSESKEDSSLAGTCPGDKEIQAVSRGFNPDAETFTPESLQQNALSVPSDLSFSPPSLDVSPTSSLLPQANVGLGAQAPNRQHFTAAMFAQTKFGSTKLKTQVKRPNRLS 222
[Close]
BLAST of Protein Tob1 vs. TrEMBL
Match: A0A1S3JX80 (protein Tob1 OS=Lingula unguis OX=7574 GN=LOC106176888 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 9.024e-21
Identity = 85/268 (31.72%), Postives = 125/268 (46.64%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFEL----LKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAW---QTPSVGPQIVQVSSE--------GCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSI 967
            +E +VA+NFILSYLYNKLPRRRV+ FGE L +      E  W       DQ ++ S      I +   +V+  I Q      +   E+ +Y PE+L ++I+P ++ Y +   +    I  S++R  ++ E     L+  L  S     K+ D    L +S   I++  + T P +S   W    +P+  P                  FI +    P FT A+FAQTKFGSTKLKS  KR +R+S    QQ+     +P      P  
Sbjct:  102 VEVSVALNFILSYLYNKLPRRRVNCFGEELAKGFHKKFEGHWYP-----DQPFKGSAFRC--IPVNGEKVDPTIIQAVYNSGLTTEEVFTYLPEDLTIWIDPAEVSYRVG-EKGVVKILYSNKRDTDLLEGPDKELQTSLFSSDTQTFKTID---SLSASVGNISI--SPTTPTASG-GWPNTASPAASPTWPVSPVSPPSSTTIFNPFIPRVQG-PTFTTAAFAQTKFGSTKLKSITKRPTRLSPTEFQQKQHTSAVPPPACTAPGF 354
[Close]
BLAST of Protein Tob1 vs. TrEMBL
Match: A0A2C9JVZ3 (Anti_prolifrtn domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106070319 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.253e-20
Identity = 92/291 (31.62%), Postives = 133/291 (45.70%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI-------------KPAESNFDIWRSDQRKYEIFE-------------LLKEGLLKSPEYERKSQDQEQEL----------------ISSTEKITLRTTGTLPYSSTF--AWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISS------YLAQQRSLVG 931
            +E +VA+NF++SYLYNKLPRRRVD+FGE LE  L+   +  W  +     Q  + S  F    G G ++V+ ++   A    +DI E+ SY PE+L L+I+P ++ Y I             K  ESN ++ R  Q     F                 + L  +P+      D  Q                   SS       +T T P S+ F  A  +P++ P  +     G   ++     + TAA FAQTKFGSTKLK+Q+KR +R+S       +  QQRS  G
Sbjct:    3 VEISVALNFVISYLYNKLPRRRVDLFGEELEVGLKEKFQGHWYPE-----QPSKGS-GFRCLKGNG-DEVDPVLVYAAVKAGLDITEVRSYLPEDLTLWIDPSEVSYKIGEKGPVKVLYSDRKDEESNENVDREVQAASRTFNPEAQTFTPESHQAFTPDLLAFTPDVAAFCSDSTQSFQPIDSLSSSLSNLSLSPSSPVPPGTWSTATSPSSNLFSSAMASPNIVPPSLVGPRAGNLHRQ-----HMTAAMFAQTKFGSTKLKTQVKRPNRLSPTEFGNYFKQQQRSYAG 281
[Close]
BLAST of Protein Tob1 vs. TrEMBL
Match: Q6IQP8 (Transducer of ERBB2, 1b OS=Danio rerio OX=7955 GN=tob1b PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 5.802e-18
Identity = 72/231 (31.17%), Postives = 118/231 (51.08%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQE-QELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRIS 898
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+ L+   E  W       D+ Y+ S     ++G    +V+ ++ + A    +DI ++ +  P++L ++I+P ++ Y I   +    +   D       EL KE         R S + E Q  +  +E + + +  + P    F  Q+ SV P  +         Q+S+    FT ASFA TKFGSTK+K+  +   +++
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERQLKKKYEGHWYP-----DKPYKGSGFRCIHVG---EKVDPVVEEAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQIG-EKGAVKVLYVDDNSENGSELDKE--------IRNSFNPEAQVFMPISEPVGVSSESSSPSPPPFG-QSASVSPSFMP--------QRSAQPLTFTTASFAATKFGSTKMKNSGRNGGKVA 207
[Close]
BLAST of Protein Tob1 vs. Ensembl Cavefish
Match: tob1a (transducer of ERBB2, 1a [Source:ZFIN;Acc:ZDB-GENE-031030-4])

HSP 1 Score: 80.8777 bits (198), Expect = 2.986e-16
Identity = 41/109 (37.61%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+ L+   E  W       D+ Y+ S     ++G    +V+ ++ Q A    +DI ++ +  P++L ++I+P ++ Y I
Sbjct:   18 LEIQVALNFIISYLYNKLPRRRVNIFGEELERQLKQKYEGHWYP-----DKPYKGSGFRCIHVG---EKVDPVVEQAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQI 118
[Close]
BLAST of Protein Tob1 vs. Ensembl Cavefish
Match: TOB2 (transducer of ERBB2, 2 [Source:HGNC Symbol;Acc:HGNC:11980])

HSP 1 Score: 80.1073 bits (196), Expect = 4.902e-16
Identity = 64/233 (27.47%), Postives = 102/233 (43.78%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITL-----RTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTP--------NFTAASFAQTKFGSTKLK 868
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE++L S  E  W  +       +        ++G   + +   ++  A    +D  E+ +  P  L ++I+P ++ Y I   E                 ++K   ++ P       +     +    K+ L     ++ G  P +  F      V P ++  ++       SS +P         FT ASFA TKFGSTK+K
Sbjct:    3 LEVKVALNFIVSYLYNKLPRRRADLFGEELERILVSRFEGHWYPEAPLRGSAFRC-----LHLGAPRDPI---VDLAARRSGLDTEEVRANVPPELSIWIDPYEVSYQI--GEKG---------------VVKVLYIEDPPGLGVEGEASDSAVCPVSKVDLELDEGKSLGFNPDAQVFVPIGGHVSPVLLNPNTHS---NASSPSPPTTAPQPITFTTASFAATKFGSTKMK 207
[Close]
BLAST of Protein Tob1 vs. Ensembl Cavefish
Match: tob1b (transducer of ERBB2, 1b [Source:ZFIN;Acc:ZDB-GENE-040426-2151])

HSP 1 Score: 77.7962 bits (190), Expect = 2.008e-15
Identity = 39/109 (35.78%), Postives = 67/109 (61.47%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+ L+   E  W       D+ ++ S     ++G    +V+ ++ + A    +DI ++ +  P++L ++I+P ++ Y I
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERQLKKKYEGHWYP-----DKPFKGSGYRCIHVG---EKVDPVVEEAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQI 103
[Close]
BLAST of Protein Tob1 vs. Ensembl Nematostella
Match: EDO38535 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCK5])

HSP 1 Score: 63.5438 bits (153), Expect = 1.347e-12
Identity = 34/108 (31.48%), Postives = 55/108 (50.93%), Query Frame = 2 
Query:  212 EATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSI 535
            E  V+VNF++++LYNKLPRRRVD+FGE L   L    +  W          Y    +        T+ ++ ++   A    + I ++ +  PE L L+I+P ++ Y I
Sbjct:    1 EIQVSVNFLMNFLYNKLPRRRVDLFGEELAMKLTKKFDGHWYPNKPTKGSGYRCILI--------THYLDPILAAAARESGLKIDDVKANLPEKLCLWIDPHEVSYRI 100
[Close]
BLAST of Protein Tob1 vs. Ensembl Medaka
Match: TOB2 (transducer of ERBB2, 2 [Source:NCBI gene;Acc:101169611])

HSP 1 Score: 80.8777 bits (198), Expect = 1.818e-16
Identity = 65/223 (29.15%), Postives = 96/223 (43.05%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPN---FTAASFAQTKFGSTKLK 868
            +E  VA+NFI+SYLYNKLPRRR D+FGE LE++L S  +  W  +       +         I +G  Q + ++   A    +D  E+ +  P  L ++I+P ++ Y I                       ++G +K    E   + +E            +T G  P +  F      V P ++   S       S   P    FT ASFA TKFGSTK+K
Sbjct:    3 LEVKVALNFIVSYLYNKLPRRRADLFGEELEKILVSRFDGHWYPEAPMRGSAFRC-------IHLGA-QRDPVVELAAKRSGLDTEEVRANVPAELSVWIDPYEVSYQIG----------------------EQGPVKLLYLEDPPEAEEG-----------KTMGFNPDAQVFVPVGSQVSPALMPSLSSSPTPLSSQPCPGPMTFTTASFAATKFGSTKMK 184
[Close]
BLAST of Protein Tob1 vs. Ensembl Medaka
Match: tob1b (transducer of ERBB2, 1b [Source:ZFIN;Acc:ZDB-GENE-040426-2151])

HSP 1 Score: 67.0106 bits (162), Expect = 9.941e-12
Identity = 65/220 (29.55%), Postives = 110/220 (50.00%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLK 868
            +E  VA+NFI+SYLYNKLPRRRV++FGE LE+ L+   E  W  +     + Y+ S     ++G    +V+ ++ + A    +DI ++ +  P++L ++I+P ++ Y I   +    +   D       EL +E         + S + E ++          ++ +   S  F  Q+ +V P          F+ +S+    FT A+FA TKFGSTK+K
Sbjct:    3 LEIQVALNFIISYLYNKLPRRRVNIFGEELERQLRRKYEGHWYPE-----KPYKGSGFRCIHVG---EKVDPVVERAAKESGLDIEDVRNNLPQDLSVWIDPFEVSYQIG-EKGAVKVLYVDDNGENGSELDRE--------IKNSFNPEAQVFMPISDPAGASSESSSPSPPFG-QSAAVSPS---------FMPRSAQPLTFTTATFAATKFGSTKMK 195
[Close]
BLAST of Protein Tob1 vs. Planmine SMEST
Match: SMESG000080545.1 (SMESG000080545.1)

HSP 1 Score: 538.11 bits (1385), Expect = 0.000e+0
Identity = 304/367 (82.83%), Postives = 307/367 (83.65%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVAXXXXXXXXXXXXXXXXXXPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD 1309
            MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYE SYVFTFYIGIGTNQVNSMIN                                               Q +  IF     G L + +YERKSQ QEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD
Sbjct:    1 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEVSYVFTFYIGIGTNQVNSMIN-----------------------------------------------QNRISIF-----GDLINEKYERKSQVQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD 315
[Close]
BLAST of Protein Tob1 vs. Planmine SMEST
Match: SMESG000080545.1 (SMESG000080545.1)

HSP 1 Score: 500.36 bits (1287), Expect = 6.815e-177
Identity = 290/367 (79.02%), Postives = 295/367 (80.38%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVAXXXXXXXXXXXXXXXXXXPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD 1309
            MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYE SYV  F   IG                                                      ++Q +  IF     G L + +YERKSQ QEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD
Sbjct:    1 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEVSYV--FTFYIGIG----------------------------------------------------TNQNRISIF-----GDLINEKYERKSQVQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD 308
[Close]
BLAST of Protein Tob1 vs. Planmine SMEST
Match: SMESG000080545.1 (SMESG000080545.1)

HSP 1 Score: 403.675 bits (1036), Expect = 3.489e-139
Identity = 227/233 (97.42%), Postives = 229/233 (98.28%), Query Frame = 2 
Query:  611 GLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVAXXXXXXXXXXXXXXXXXXPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD 1309
            G L + +YERKSQ QEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD
Sbjct:   61 GDLINEKYERKSQVQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALEPSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHSPPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVTFAVTTIEEQFSWPRNTD 293

HSP 2 Score: 119.398 bits (298), Expect = 5.670e-30
Identity = 54/54 (100.00%), Postives = 54/54 (100.00%), Query Frame = 2 
Query:  209 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYE 370
            MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYE
Sbjct:    1 MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYE 54
[Close]
BLAST of Protein Tob1 vs. Planmine SMEST
Match: SMESG000063539.1 (SMESG000063539.1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.485e-9
Identity = 58/222 (26.13%), Postives = 95/222 (42.79%), Query Frame = 2 
Query:  206 LMEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHDQTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPEN-LILFIEPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQEQELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSSTPNFTAASFAQTKFGSTKLK 868
            + E  VAV +I+S LY+ LPRRRVD F   L  LL+  +  FW       +Q +   +   F +  GT   + ++   A+   I+  E+ ++ P++ L ++I+PG++ Y +                                      DQ  E+     K   R     P  +   +Q+        + +++  F    S  P+FT +SFA+TKFGSTKLK
Sbjct:    2 ISEIAVAVTYIVSPLYSNLPRRRVDDFAIELNSLLKLKIAPFWFP-----NQPFRGIHQRCFRVN-GT-YADPILYIAASKSGINQEELFNFLPKDELKIWIDPGEVTYQVG-------------------------------------DQNSEI-----KCLFRCN---PVVNEHNFQSSIYN----ETNNQSNFYLPKSLVPSFTVSSFAKTKFGSTKLK 167
The following BLAST results are available for this feature:
BLAST of Protein Tob1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TOB29.091e-1729.09transducer of ERBB2, 2 [Source:HGNC Symbol;Acc:HGN... [more]
TOB11.231e-1538.53transducer of ERBB2, 1 [Source:HGNC Symbol;Acc:HGN... [more]
TOB11.231e-1538.53transducer of ERBB2, 1 [Source:HGNC Symbol;Acc:HGN... [more]
TOB21.609e-1335.51transducer of ERBB2, 2 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Protein Tob1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fog-39.296e-922.27FOG-3 protein; Feminization Of Germline [Source:U... [more]
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BLAST of Protein Tob1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Tob8.666e-1433.94gene:FBgn0028397 transcript:FBtr0074255[more]
Tob8.666e-1433.94gene:FBgn0028397 transcript:FBtr0310446[more]
Tob1.356e-1333.94gene:FBgn0028397 transcript:FBtr0301652[more]
Tob1.420e-1333.94gene:FBgn0028397 transcript:FBtr0310317[more]
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BLAST of Protein Tob1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tob1b3.380e-2131.17transducer of ERBB2, 1b [Source:ZFIN;Acc:ZDB-GENE-... [more]
tob1a3.082e-1637.61transducer of ERBB2, 1a [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of Protein Tob1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tob11.728e-1930.00transducer of ERBB2, 1 [Source:NCBI gene;Acc:54936... [more]
tob24.619e-1636.70transducer of ERBB2, 2 [Source:NCBI gene;Acc:54896... [more]
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BLAST of Protein Tob1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Tob23.239e-1335.78transducer of ERBB2, 2 [Source:MGI Symbol;Acc:MGI:... [more]
Tob27.576e-1264.29transducer of ERBB2, 2 [Source:MGI Symbol;Acc:MGI:... [more]
Tob11.356e-929.13transducer of ErbB-2.1 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of Protein Tob1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q14106|TOB2_HUMAN4.366e-1629.09Protein Tob2 OS=Homo sapiens OX=9606 GN=TOB2 PE=1 ... [more]
sp|P50616|TOB1_HUMAN5.912e-1538.53Protein Tob1 OS=Homo sapiens OX=9606 GN=TOB1 PE=1 ... [more]
sp|Q8R5K6|TOB1_RAT1.179e-1437.61Protein Tob1 OS=Rattus norvegicus OX=10116 GN=Tob1... [more]
sp|Q9JM55|TOB2_MOUSE2.266e-1235.78Protein Tob2 OS=Mus musculus OX=10090 GN=Tob2 PE=2... [more]
sp|Q61471|TOB1_MOUSE9.484e-929.13Protein Tob1 OS=Mus musculus OX=10090 GN=Tob1 PE=1... [more]
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BLAST of Protein Tob1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7TUR95.403e-2531.03Anti_prolifrtn domain-containing protein (Fragment... [more]
A0A433U0S27.804e-2230.52Anti_prolifrtn domain-containing protein (Fragment... [more]
A0A1S3JX809.024e-2131.72protein Tob1 OS=Lingula unguis OX=7574 GN=LOC10617... [more]
A0A2C9JVZ31.253e-2031.62Anti_prolifrtn domain-containing protein OS=Biomph... [more]
Q6IQP85.802e-1831.17Transducer of ERBB2, 1b OS=Danio rerio OX=7955 GN=... [more]
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BLAST of Protein Tob1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tob1a2.986e-1637.61transducer of ERBB2, 1a [Source:ZFIN;Acc:ZDB-GENE-... [more]
TOB24.902e-1627.47transducer of ERBB2, 2 [Source:HGNC Symbol;Acc:HGN... [more]
tob1b2.008e-1535.78transducer of ERBB2, 1b [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of Protein Tob1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein Tob1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein Tob1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO385351.347e-1231.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein Tob1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TOB21.818e-1629.15transducer of ERBB2, 2 [Source:NCBI gene;Acc:10116... [more]
tob1b9.941e-1229.55transducer of ERBB2, 1b [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of Protein Tob1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000080545.10.000e+082.83SMESG000080545.1[more]
SMESG000080545.16.815e-17779.02SMESG000080545.1[more]
SMESG000080545.13.489e-13997.42SMESG000080545.1[more]
SMESG000063539.12.485e-926.13SMESG000063539.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007982 ID=SMED30007982|Name=Protein Tob1|organism=Schmidtea mediterranea sexual|type=transcript|length=1562bp
GAATTTTTTATTTATTTTAAAACTTTTTAATAAACCTAAGCTGTATAGAT
TACACTTTGGTACATAAATTAGCTGGTTATAAATGATCTATATATTTTCA
AGGACATTTTACAATTGGATATTTTACAAATCGGAGAGCCTGGAATTATC
AATGAATTTTTAACTGTTATTTTCTAGATGAGCTAATTTGTATAATCTAG
AGTAACTGATGGAAGCAACAGTTGCTGTTAATTTTATATTGTCATATCTT
TATAATAAACTGCCGCGACGAAGAGTTGATATGTTTGGTGAAATGTTAGA
GCAATTATTGCAATCGAATTTGGAAAAGTTCTGGGTTTCACAATTCAGCA
AACATGATCAAACGTATGAGGCATCGTACGTTTTTACATTCTATATAGGA
ATTGGAACGAATCAAGTTAATTCTATGATTAACCAAGTTGCAGCTGCGTG
TTTAATTGACATTTACGAGATTCTTTCATATTTTCCTGAAAATCTCATTC
TGTTTATTGAACCGGGAAAACTTCAATATTCTATTAAGCCGGCAGAATCG
AATTTCGATATTTGGAGATCTGATCAACGAAAGTACGAAATATTTGAACT
ATTAAAAGAAGGATTATTAAAATCTCCAGAATATGAAAGAAAATCACAAG
ATCAAGAGCAAGAATTGATTTCATCGACTGAGAAAATAACTTTAAGAACA
ACCGGAACTTTGCCATATTCAAGCACATTTGCGTGGCAAACTCCATCCGT
GGGCCCTCAAATTGTCCAAGTTAGTTCAGAAGGATGTTTCATTCAAAAAT
CTAGCTCTACGCCAAATTTTACTGCAGCTTCATTCGCTCAGACGAAATTC
GGATCCACAAAGCTCAAATCACAACTAAAACGATCAAGTCGGATAAGTAG
TTATCTTGCTCAACAACGATCACTAGTAGGAGTTATTCCTGCTACAATTG
CACTGGAGCCATCAATTAAATCACCCAAAGTCGCTACATCTGATTCTCCT
TCTAGTTTGTCACCGTTTACGAGTTCCTATTTTGAAAGTCCAACATCAAT
GTTAAGTCAAAGATCTATTTCATTGTCTCCTTGTATTAGGCCACAACGAG
GTCATAGTCCGCCGTTTTCGTTCAATATTCCACTCATTTTACCACCGTTT
ACGTCTGGTACAGATATCATTGGTGCTCCACAATTTCCAATTTCTTCATC
AACTCAAATTTTACCGCCTACAATACAGATTACACGAGAAGACCCAACAC
CAGTCACTTTTGCTGTTACTACAATCGAAGAACAGTTCTCATGGCCTAGG
AATACGGATTAGGAACTATTTTTTTCTTTGGTGAAGCTTCTATAATGTTA
CGCATATCGTTTGCAATTCTCTCTCTCTCTCTCCCATATGTCTTTTTAAA
TGGGATTTGGTATCTCCAGATAAGATTTATCATTTGATCAACTAGAACAT
TTTCTCTTTTGTTAAAGAAATAATTATATGGAAATATATCAACTTTATAA
TAACAAACTCTTTCAATTTTGTACAAAAGCTTTACTGTGATACAATTTAA
GAAAACAGTGGA
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protein sequence of SMED30007982-orf-1

>SMED30007982-orf-1 ID=SMED30007982-orf-1|Name=SMED30007982-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=368bp
MEATVAVNFILSYLYNKLPRRRVDMFGEMLEQLLQSNLEKFWVSQFSKHD
QTYEASYVFTFYIGIGTNQVNSMINQVAAACLIDIYEILSYFPENLILFI
EPGKLQYSIKPAESNFDIWRSDQRKYEIFELLKEGLLKSPEYERKSQDQE
QELISSTEKITLRTTGTLPYSSTFAWQTPSVGPQIVQVSSEGCFIQKSSS
TPNFTAASFAQTKFGSTKLKSQLKRSSRISSYLAQQRSLVGVIPATIALE
PSIKSPKVATSDSPSSLSPFTSSYFESPTSMLSQRSISLSPCIRPQRGHS
PPFSFNIPLILPPFTSGTDIIGAPQFPISSSTQILPPTIQITREDPTPVT
FAVTTIEEQFSWPRNTD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR036054BTG-like_sf
IPR015676Tob1/2
IPR002087Anti_prolifrtn
Vocabulary: biological process
TermDefinition
GO:0008285negative regulation of cell proliferation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002087Anti-proliferative proteinSMARTSM00099btg1_nrcoord: 1..112
e-value: 7.9E-4
score: 11.2
IPR002087Anti-proliferative proteinPFAMPF07742BTGcoord: 2..112
e-value: 3.9E-15
score: 55.7
IPR036054BTG-like domain superfamilyGENE3DG3DSA:1.20.120.1120coord: 1..127
e-value: 1.8E-17
score: 64.9
IPR036054BTG-like domain superfamilySUPERFAMILYSSF160696BTG domain-likecoord: 2..122
NoneNo IPR availablePANTHERPTHR17537:SF5TOB, ISOFORM Acoord: 2..282
IPR015676Tob1/2PANTHERPTHR17537TRANSDUCER OF ERBB2 TOBcoord: 2..282