Transcription initiation factor IIF subunit alpha

Overview
NameTranscription initiation factor IIF subunit alpha
Smed IDSMED30007951
Length (bp)4280
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcription initiation factor IIF subunit alpha (SMED30007951) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcription initiation factor IIF subunit alpha (SMED30007951) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcription initiation factor IIF subunit alpha (SMED30007951) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcription initiation factor IIF subunit alpha (SMED30007951) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007951

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30007951SMESG000056085.1 dd_Smed_v4_6621_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007951SMESG000056085.1 dd_Smed_v4_6621_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30007951SMESG000056085.1 dd_Smed_v4_6621_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30007951SMESG000056085.1 dd_Smed_v4_6621_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007951SMESG000056085.1 Contig41241newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007951SMESG000056085.1 Contig41241uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30007951SMESG000056085.1 dd_Smed_v6_6621_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Human
Match: GTF2F1 (general transcription factor IIF subunit 1 [Source:HGNC Symbol;Acc:HGNC:4652])

HSP 1 Score: 58.5362 bits (140), Expect = 7.312e-8
Identity = 37/134 (27.61%), Postives = 71/134 (52.99%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQP 713
            K+ +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F +  +  FEA  V +WY   P
Sbjct:   24 KKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTP 148
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Human
Match: GTF2F1 (general transcription factor IIF subunit 1 [Source:HGNC Symbol;Acc:HGNC:4652])

HSP 1 Score: 57.3806 bits (137), Expect = 1.239e-7
Identity = 37/134 (27.61%), Postives = 71/134 (52.99%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQP 713
            K+ +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F +  +  FEA  V +WY   P
Sbjct:   84 KKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTP 208
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Fly
Match: TfIIFalpha (gene:FBgn0010282 transcript:FBtr0081719)

HSP 1 Score: 50.8322 bits (120), Expect = 9.003e-6
Identity = 35/121 (28.93%), Postives = 62/121 (51.24%), Query Frame = 3 
Query:  459 PIFGAGTEFCKEARDEAWQRKFALYKKKSESK-DPINLSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKL--EDSIEEYNKVAKKTESTSIIFSKR 812
            P FGAG+E+ ++ R+EA ++KF +  +K   +  P  L +G     K     +  + EN+ + VF  + +   EA  + +WY  QP  I R+  L  E++ +E+ +  K     S++  KR
Sbjct:   90 PKFGAGSEYNRDQREEARRKKFGIIARKYRPEAQPWILKVGGKTGKKFKGIREGGVGENAAFYVFTHAPDGAIEAYPLTEWYNFQP--IQRYKSLSAEEAEQEFGRRKKVMNYFSLMLRKR 208
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Zebrafish
Match: gtf2f1 (general transcription factor IIF, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-030131-4557])

HSP 1 Score: 55.8398 bits (133), Expect = 2.535e-7
Identity = 36/117 (30.77%), Postives = 66/117 (56.41%), Query Frame = 3 
Query:  468 GAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
             AG+EF K+ R+EA ++K+ +  K+ + +D P  L +  G+  +++K +K+  + EN+ Y +F +S++  FEA  V  WY   P+   R    E++ EE+ +  K     SI+  +R
Sbjct:   69 AAGSEFGKKQREEARRKKYGIITKEFKVEDQPWILKVN-GKAGRRFKGIKKGGVTENASYYIFTQSADGAFEAFPVHAWYNFTPQAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 184
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Zebrafish
Match: gtf2f1 (general transcription factor IIF, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-030131-4557])

HSP 1 Score: 55.8398 bits (133), Expect = 2.535e-7
Identity = 36/117 (30.77%), Postives = 66/117 (56.41%), Query Frame = 3 
Query:  468 GAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
             AG+EF K+ R+EA ++K+ +  K+ + +D P  L +  G+  +++K +K+  + EN+ Y +F +S++  FEA  V  WY   P+   R    E++ EE+ +  K     SI+  +R
Sbjct:   69 AAGSEFGKKQREEARRKKYGIITKEFKVEDQPWILKVN-GKAGRRFKGIKKGGVTENASYYIFTQSADGAFEAFPVHAWYNFTPQAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 184
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Zebrafish
Match: gtf2f1 (general transcription factor IIF, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-030131-4557])

HSP 1 Score: 55.8398 bits (133), Expect = 3.661e-7
Identity = 36/117 (30.77%), Postives = 66/117 (56.41%), Query Frame = 3 
Query:  468 GAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
             AG+EF K+ R+EA ++K+ +  K+ + +D P  L +  G+  +++K +K+  + EN+ Y +F +S++  FEA  V  WY   P+   R    E++ EE+ +  K     SI+  +R
Sbjct:   69 AAGSEFGKKQREEARRKKYGIITKEFKVEDQPWILKVN-GKAGRRFKGIKKGGVTENASYYIFTQSADGAFEAFPVHAWYNFTPQAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 184
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Xenopus
Match: gtf2f1 (general transcription factor IIF subunit 1 [Source:NCBI gene;Acc:100124929])

HSP 1 Score: 62.7734 bits (151), Expect = 3.393e-9
Identity = 43/167 (25.75%), Postives = 85/167 (50.90%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            KR  ++ FN          N   +E++L++K+M+         +  + P  GAG+E+ ++ R+E+ ++K+ +  K+ + +D   L    G+  +KYK VK+  + EN+ Y +F + ++  FEA  V +WY   P    R    E++ +E+ +  K     +I+  +R
Sbjct:   24 KRYSLMAFNAADKVDFSTWNQARMERDLSAKKMY---------QDEEMPESGAGSEYNRKQREESRRKKYGIILKEFKLEDQPWLLRVNGKAGRKYKGVKKGGVTENASYYIFTQCADGAFEAFPVSNWYNFTPLAKHRTLTAEEAEQEWERRNKVLNHFTIMQQRR 181
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Xenopus
Match: gtf2f1 (general transcription factor IIF subunit 1 [Source:NCBI gene;Acc:100124929])

HSP 1 Score: 62.3882 bits (150), Expect = 4.114e-9
Identity = 43/167 (25.75%), Postives = 85/167 (50.90%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            KR  ++ FN          N   +E++L++K+M+         +  + P  GAG+E+ ++ R+E+ ++K+ +  K+ + +D   L    G+  +KYK VK+  + EN+ Y +F + ++  FEA  V +WY   P    R    E++ +E+ +  K     +I+  +R
Sbjct:   24 KRYSLMAFNAADKVDFSTWNQARMERDLSAKKMY---------QDEEMPESGAGSEYNRKQREESRRKKYGIILKEFKLEDQPWLLRVNGKAGRKYKGVKKGGVTENASYYIFTQCADGAFEAFPVSNWYNFTPLAKHRTLTAEEAEQEWERRNKVLNHFTIMQQRR 181
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Mouse
Match: Gtf2f1 (general transcription factor IIF, polypeptide 1 [Source:MGI Symbol;Acc:MGI:1923848])

HSP 1 Score: 65.4698 bits (158), Expect = 3.601e-10
Identity = 69/269 (25.65%), Postives = 132/269 (49.07%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGK---------ERYYEESDVDDHQGDEVDYMTHSSS 1091
            KR +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F + ++  FEA  V++WY   P    R    E++ EE+ +  K     SI+  +R   +  +  + +K KR+  + S++     +    +D ++ SD +D   +E  +  K        + G+         +  +E+SD  D +G EVDYM+  SS
Sbjct:   24 KRYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTAYYIFTQCADGAFEAFPVQNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRSRKKPSELR----IHDLEDDLEMSSDASDASGEEGSRTSKAKKKAPVTKAGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSS 279
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. UniProt/SwissProt
Match: sp|Q6AY96|T2FA_RAT (General transcription factor IIF subunit 1 OS=Rattus norvegicus OX=10116 GN=Gtf2f1 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.371e-9
Identity = 69/269 (25.65%), Postives = 132/269 (49.07%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGK---------ERYYEESDVDDHQGDEVDYMTHSSS 1091
            KR +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F + ++  FEA  V++WY   P    R    E++ EE+ +  K     SI+  +R   +  +  + +K KR+  + S++     +    +D ++ SD +D   +E  +  K        + G+         +  +E+SD  D +G EVDYM+  SS
Sbjct:   24 KRYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTAYYIFTQCADGAFEAFPVQNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRSRKKPSELR----IHDLEDDLEMSSDASDASGEEGSRASKAKKKAPVTKAGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSS 279
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. UniProt/SwissProt
Match: sp|Q3THK3|T2FA_MOUSE (General transcription factor IIF subunit 1 OS=Mus musculus OX=10090 GN=Gtf2f1 PE=1 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 2.519e-9
Identity = 69/269 (25.65%), Postives = 132/269 (49.07%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGK---------ERYYEESDVDDHQGDEVDYMTHSSS 1091
            KR +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F + ++  FEA  V++WY   P    R    E++ EE+ +  K     SI+  +R   +  +  + +K KR+  + S++     +    +D ++ SD +D   +E  +  K        + G+         +  +E+SD  D +G EVDYM+  SS
Sbjct:   24 KRYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTAYYIFTQCADGAFEAFPVQNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRSRKKPSELR----IHDLEDDLEMSSDASDASGEEGSRTSKAKKKAPVTKAGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSS 279
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. UniProt/SwissProt
Match: sp|Q04870|T2FA_XENLA (General transcription factor IIF subunit 1 OS=Xenopus laevis OX=8355 GN=gtf2f1 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 5.354e-8
Identity = 43/168 (25.60%), Postives = 86/168 (51.19%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            KR  ++ FN          N   +E++L++K+M+ + +          P  GAG+E+ ++ R+E+ ++K+ +  ++ +  D P  L +  G+  +KYK VK+  + EN+ Y +F + ++  FEA  V +WY   P    R    E++ +E+ +  K     +I+  +R
Sbjct:   24 KRYSLMAFNAADKVDFSTWNQARMERDLSAKKMYQEEEM---------PESGAGSEYNRKQREESRRKKYGIILREFKVDDQPWILRVN-GKAGRKYKGVKKGGVTENASYFIFTQCADGAFEAFPVSNWYNFTPVAKHRTLTAEEAEQEWERRNKVLNHFTIMQQRR 181
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. UniProt/SwissProt
Match: sp|Q5EA53|T2FA_BOVIN (General transcription factor IIF subunit 1 OS=Bos taurus OX=9913 GN=GTF2F1 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.881e-7
Identity = 37/134 (27.61%), Postives = 71/134 (52.99%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQP 713
            K+ +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F +  +  FEA  V +WY   P
Sbjct:   24 KKYNIMAFNAADKVNLTTWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTP 148
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. UniProt/SwissProt
Match: sp|P35269|T2FA_HUMAN (General transcription factor IIF subunit 1 OS=Homo sapiens OX=9606 GN=GTF2F1 PE=1 SV=2)

HSP 1 Score: 58.5362 bits (140), Expect = 3.511e-7
Identity = 37/134 (27.61%), Postives = 71/134 (52.99%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQP 713
            K+ +I+ FN          N   LE++L++K+++ + +          P  GAG+EF ++ R+EA ++K+ +  K+   +D   L    G++ +K+K +K+  + EN+ Y +F +  +  FEA  V +WY   P
Sbjct:   24 KKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEM---------PESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTP 148
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. TrEMBL
Match: T1EWJ2 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20200942 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.131e-24
Identity = 68/205 (33.17%), Postives = 97/205 (47.32%), Query Frame = 1 
Query: 1549 GISDKNQCISK--TKVPRIEWEFWVD*ILERNMVIGNVDYDALTALERQTQRKEKCPNLKKESTNSKKRIFSRIKPFATRNRIK*RQ*RFMECKTLAKESGRSEPERCRVYNKYQYRSLCEIMVRTGIRNRDASQVINAYLQYMNI----NLIMPSKLRRQRILHRTAAAISHCISNQNLLCIGFDGGIDKTRLYEGKIARITKK 2145
            GI D+N C      K+P IEW+FWVD    RNM+IG VD +    LE++ +RK       +  +   +           RN               ++ +  SE E   V N+ +Y+ LC+ + R  I NRD   ++NA L+ +++     ++  SKLRRQR   R      H  SN+ L CIGFDG  D T L E    R +KK
Sbjct:  125 GIRDRNLCTCPLPNKIPLIEWDFWVDQKTVRNMIIGGVDKETTATLEKRQKRKLMKSVWDEAQSKEVEYETEEDIDEDERNT--------------SESNYTSEEETVSVQNRNKYKELCKAVDRCKISNRDTCLIVNAVLKDLSMLSSATILDLSKLRRQRNFWRDTLVEEHMQSNKELCCIGFDGKQDVT-LVESFGCRRSKK 314

HSP 2 Score: 48.1358 bits (113), Expect = 6.131e-24
Identity = 35/118 (29.66%), Postives = 66/118 (55.93%), Query Frame = 2 
Query: 1178 TRSSC*HGIFRQ*IVLSINRLPTQQEVFNCLLWYRNQSHEASSQIQSLRDIMKLVAVDVISIWANANILTIAQHSVINKLEKLVTRGQEFEKYALAR*KASSFQATNESFQTIFYICS 1531
            TRS+  H +F Q + L  N++P+  +V     +Y NQ  +   +I SL++   ++A  V S+++ A+I TI  +S I ++ +L+ +    EKY+ A+  + +F      F+ +F IC+
Sbjct:    6 TRSATKHPLFGQLVDLPENQIPSYDDVIKA--FYYNQ--KTVLEI-SLQERYSILADRVKSVYSKASIRTIEVNSTIIRIRRLMDKVHTLEKYSDAKRTSDTFTEKLNFFKQLFDICT 118

HSP 3 Score: 23.8682 bits (50), Expect = 6.131e-24
Identity = 10/22 (45.45%), Postives = 14/22 (63.64%), Query Frame = 3 
Query: 2130 QNYKENHYVIVSFLVGPYVDHV 2195
            +  K+ HY IVS+    Y+DHV
Sbjct:  310 RRSKKEHYTIVSYPGNIYIDHV 331
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. TrEMBL
Match: A0A448X8F6 (Transcription initiation factor IIF subunit alpha OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS24063 PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 3.382e-17
Identity = 72/223 (32.29%), Postives = 118/223 (52.91%), Query Frame = 3 
Query:  180 PNKPTANLRLIQNSSSTPTNGSP---QIST-------NQVSNKDHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSK-------EMFLQRDRTQK-----IKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAK 776
            P K TA  RL+ ++ S+ TN  P   QIS+            ++    +   +E+ ++   +  EKR  +L+F+   S   NL+S        E+F+QR+   K     +     P  GAG+EF +EAR+EA  RK+ + +K    +D P   +IG+GR  +K++ V+E  + +N QY VF +     F+A  V +WYKM+PEV  RF + E++  EY+++ K
Sbjct:    3 PAKLTARQRLLGSAGSS-TNAIPISRQISSKISLPISQSPCEREPALQRTIIREMQVRV-PNRAEKRFSVLRFH--ASDRVNLSSPTGSSLPPEVFMQRENNLKQYKGGLNIIDAPTKGAGSEFGREAREEARLRKYGVTRKGYRPEDQPWLFTIGKGRTARKFRGVREGGVSDNVQYFVFCQCKEGNFDAYPVCEWYKMKPEVNYRFLREEEAEAEYSRLHK 221
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. TrEMBL
Match: A0A0F7GWW6 (General transcription factor IIF 1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.016e-15
Identity = 75/198 (37.88%), Postives = 109/198 (55.05%), Query Frame = 3 
Query:  585 RNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLE-DSIEEYNKVAKKT----ESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGKERYYEESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEAN 1163
            R  KK+K   + M  NSQY VFIR S   FEA  +++WYK+ P     F KL+ +SIEE  +   +     +S+S  F+++Y                  EE +V H   +   N D+++ SD+   E++ + K+KK+     K     + +  ESDVDDHQGDEVDYM+ SS DED L + EKEK+Y   +VD+EA+
Sbjct:    3 RYKKKFKTAPQEMNPNSQYLVFIRKSESLFEAYPLENWYKINP-----FTKLDINSIEESERDQLRKGQIFDSSSKKFTEKYKV----------------EEKEV-HKNDIEFGNEDKEL-SDSEIQEKQILNKRKKDTVFQRKRHGQGDAHAGESDVDDHQGDEVDYMSDSSCDEDVLSDGEKEKLYEAVDVDQEAD 177
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. TrEMBL
Match: A0A095A2L0 (Dehydrogenase/reductase SDR family member 12 OS=Schistosoma haematobium OX=6185 GN=MS3_10066 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.278e-13
Identity = 55/168 (32.74%), Postives = 103/168 (61.31%), Query Frame = 3 
Query:  342 EKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKI-----KTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGR-NVKKYKAVKE-SMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLT 821
            +KR  +L+F+    L+ + ++ E+F+QR+   K       T++ P  GAG+EF +EA++EA  +KF + ++  +  D P  +++G+G+ N ++++ V+E S+ EN +Y VF +  +  F+A  V  WYKM+PE+  RF + +++  EY+++  KT +   +  KR LT
Sbjct:  348 DKRFSVLRFHAADRLDLS-SAPEVFMQRENNLKAYRSLYNTTEMPDTGAGSEFGREAKEEARLKKFGVVRESYKPDDQPWLMTVGKGKANRRRFRGVREGSVSENVEYFVFCQCKDGNFDAYPVNAWYKMKPEISYRFLREDEAEAEYSRL-HKTLNLFNVMVKRKLT 513
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. TrEMBL
Match: A0A430Q762 (Transcription initiation factor TFIIF subunit alpha (Fragment) OS=Schistosoma bovis OX=6184 GN=DC041_0011334 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.385e-13
Identity = 55/168 (32.74%), Postives = 103/168 (61.31%), Query Frame = 3 
Query:  342 EKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKI-----KTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGR-NVKKYKAVKE-SMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLT 821
            +KR  +L+F+    L+ + ++ E+F+QR+   K       T++ P  GAG+EF +EA++EA  +KF + ++  +  D P  +++G+G+ N ++++ V+E S+ EN +Y VF +  +  F+A  V  WYKM+PE+  RF + +++  EY+++  KT +   +  KR LT
Sbjct:  170 DKRFSVLRFHAADRLDLS-SAPEVFMQRENNLKAYRSLYNTTEMPDTGAGSEFGREAKEEARLKKFGVVRESYKPDDQPWLMTVGKGKANRRRFRGVREGSVSENVEYFVFCQCKDGNFDAYPVNAWYKMKPEISYRFLREDEAEAEYSRL-HKTLNLFNVMVKRKLT 335
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Cavefish
Match: gtf2f1 (general transcription factor IIF, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-030131-4557])

HSP 1 Score: 60.077 bits (144), Expect = 1.229e-8
Identity = 47/190 (24.74%), Postives = 96/190 (50.53%), Query Frame = 3 
Query:  249 QISTNQVSNKDHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            ++ T  VS K      + + EI++   S    + +          +E++++++ M+ + +      T++G    AG+EF K+ R+E+ ++KF +  K+ + +D P  L +  G+  K++K +K+  + EN+ Y +F + ++  FEA  V  WY   P+   R    E++ EE+ +  K     SI+  +R
Sbjct:   30 RVLTRIVSKKHKTTADQITAEIYVHLNSPVSTRTVFQFHCPTPARMERDMSNRRMYGEEE------TAEG---AAGSEFGKKQREESRRKKFGIITKEFKVEDQPWILKVN-GKAGKRFKGLKKGGVTENASYYIFTQCADGAFEAFPVHGWYNFTPQAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 209
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Sea Lamprey
Match: gtf2f1 (general transcription factor IIF, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-030131-4557])

HSP 1 Score: 58.9214 bits (141), Expect = 6.251e-9
Identity = 37/130 (28.46%), Postives = 69/130 (53.08%), Query Frame = 3 
Query:  345 KRIDILQFN----------NDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKE-SMFENSQYCVFIRSSNDCFEAVSVKDWY 701
            KR +++ FN              +E++L +K+M+ + +          P FGAG+EF ++ R+EA ++K+ +  ++ + +D   L    G+  ++YK VK+  + EN+ Y VF + ++  FEA  V  WY
Sbjct:   25 KRFNLMAFNPGDKVDFSAWTQAKMERDLTNKKMYGEEEL---------PEFGAGSEFRRKQREEARRKKYGITLRQFKIEDQPWLLKVNGKAGRRYKGVKKGGVTENACYYVFAQCADGAFEAFPVSSWY 145
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Nematostella
Match: EDO48760 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIQ9])

HSP 1 Score: 51.6026 bits (122), Expect = 2.545e-7
Identity = 38/119 (31.93%), Postives = 59/119 (49.58%), Query Frame = 3 
Query:  459 PIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            P  GAG+E+ +E R+EA +R+     K ++ K  P  L IG G+  KK   + +    NS Y +  +  +  FEAV V  WY   P +       E++ EE+N+  K     S++  KR
Sbjct:   52 PTHGAGSEYGREWREEARKRRRGYMPKTADPKSAPWALKIG-GKAGKKEAGIND----NSNYYILTQCPDGAFEAVPVDSWYNFTPNIHYHTLNAEEAEEEFNRRDKTVNFFSLMVKKR 165
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Medaka
Match: gtf2f1 (general transcription factor IIF subunit 1 [Source:NCBI gene;Acc:101156554])

HSP 1 Score: 55.8398 bits (133), Expect = 2.634e-7
Identity = 43/168 (25.60%), Postives = 85/168 (50.60%), Query Frame = 3 
Query:  345 KRIDILQFNND----------GSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            K+ +I+ FN+             +E++L+++ ++ + +          P  GAG+EF K+ R+EA ++K+ +  ++ + +D P  L +  G+  K++K +K+  + EN+ Y +F +  +  FEA  V  WY   P    R    E++ EE+ +  K     SI+  +R
Sbjct:   27 KKYNIMAFNSGDKVNCSAWTQARMERDLSARRIYGEEEL---------PEGGAGSEFGKKQREEARRKKYGIVTREFKVEDQPWILKV-NGKAGKRFKGIKKGGVTENASYYIFTQCPDGAFEAFPVHGWYNFTPVAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 184
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Medaka
Match: gtf2f1 (general transcription factor IIF subunit 1 [Source:NCBI gene;Acc:101156554])

HSP 1 Score: 55.8398 bits (133), Expect = 3.040e-7
Identity = 43/168 (25.60%), Postives = 85/168 (50.60%), Query Frame = 3 
Query:  345 KRIDILQFNND----------GSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            K+ +I+ FN+             +E++L+++ ++ + +          P  GAG+EF K+ R+EA ++K+ +  ++ + +D P  L +  G+  K++K +K+  + EN+ Y +F +  +  FEA  V  WY   P    R    E++ EE+ +  K     SI+  +R
Sbjct:   23 KKYNIMAFNSGDKVNCSAWTQARMERDLSARRIYGEEEL---------PEGGAGSEFGKKQREEARRKKYGIVTREFKVEDQPWILKV-NGKAGKRFKGIKKGGVTENASYYIFTQCPDGAFEAFPVHGWYNFTPVAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 180
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Medaka
Match: gtf2f1 (general transcription factor IIF subunit 1 [Source:NCBI gene;Acc:101156554])

HSP 1 Score: 55.8398 bits (133), Expect = 3.075e-7
Identity = 43/168 (25.60%), Postives = 85/168 (50.60%), Query Frame = 3 
Query:  345 KRIDILQFNND----------GSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKD-PINLSIGRGRNVKKYKAVKES-MFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKR 812
            K+ +I+ FN+             +E++L+++ ++ + +          P  GAG+EF K+ R+EA ++K+ +  ++ + +D P  L +  G+  K++K +K+  + EN+ Y +F +  +  FEA  V  WY   P    R    E++ EE+ +  K     SI+  +R
Sbjct:   27 KKYNIMAFNSGDKVNCSAWTQARMERDLSARRIYGEEEL---------PEGGAGSEFGKKQREEARRKKYGIVTREFKVEDQPWILKV-NGKAGKRFKGIKKGGVTENASYYIFTQCPDGAFEAFPVHGWYNFTPVAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRR 184
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Planmine SMEST
Match: SMESG000056085.1 (SMESG000056085.1)

HSP 1 Score: 681.019 bits (1756), Expect = 0.000e+0
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 3 
Query:  111 MNINIPILLANSNNKVIITSSNVPNKPTANLRLIQNSSSTPTNGSPQISTNQVSNKDHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGKERYYEESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEANSKS 1172
            MNINIPILLANSNNKVIITSSNVPNKPTANLRLIQNSSSTPTNGSPQISTNQVSNKDHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTEVDEVVKAKKVKRNSDEESDVYHGPGLIITNNDQDIFSDTTDDEEKEVMKKKKNAALLSKIRLGKERYYEESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEANSKS
Sbjct:    1 MNINIPILLANSNNKVIITSSNVPNKPTANLRLIQNSSSTPTNGSPQISTNQVSNKDHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPINLSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTEVDEVVKAKKVKRNSDEESDVYHGPGLIITNNDQDIFSDTTDDEEKEVMKKKKNAALLSKIRLGKERYYEESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEANSKS 354

HSP 2 Score: 199.519 bits (506), Expect = 3.022e-54
Identity = 169/171 (98.83%), Postives = 170/171 (99.42%), Query Frame = 2 
Query: 3704 TKPDDELSNMDEEVNANANTEESDSKTIKEIEVXXXXXXXXXXXXXXXXXXXXXXXXXXXTALKQKLSTTINKNIASQMKXXXXXXXXXXXXHFEDISRKFKQDISQNNRIIQEMYKYLNRKPXXXXXXXXXXXXXXXXXXESQKLFDSLLDKINTITHIVNGEKLFSIIN 4216
            +K DDELSNMDEEVNANANTEESDSKTIKEIEVESNADSDSSNDSSPMSSIDESDDEDSNTALKQKLSTTINKNIASQMKLNSRSQSNSNLSHFEDISRKFKQDISQNNRIIQEMYKYLNRKPISKSNLFKKLILKKLLSNESQKLFDSLLDKINTITHIVNGEKLFSIIN
Sbjct:  352 SKSDDELSNMDEEVNANANTEESDSKTIKEIEVESNADSDSSNDSSPMSSIDESDDEDSNTALKQKLSTTINKNIASQMKLNSRSQSNSNLSHFEDISRKFKQDISQNNRIIQEMYKYLNRKPISKSNLFKKLILKKLLSNESQKLFDSLLDKINTITHIVNGEKLFSIIN 522
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Planmine SMEST
Match: SMESG000055295.1 (SMESG000055295.1)

HSP 1 Score: 124.79 bits (312), Expect = 2.184e-30
Identity = 104/304 (34.21%), Postives = 155/304 (50.99%), Query Frame = 3 
Query:  285 EFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQK-IKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPIN--LSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLE-DSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGKERYYE-ESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEANSKSAQL 1181
            EF + YSK++F+KFR    ++++DILQF  D +     +   MF+Q+   +K       P FG G+ + KE            + K+  +K P    L+I +G+  +K+K   + M    +Y +F+R S   FEA  +++WYKM     ++F KL+ +SIEE  +   K E T+   S R+L +                  D         T N  + FSD+ +  EKE ++  K  A+ ++ R GK   YE ESDVDDHQGDEVDYM+ SS DE+ L   E+EKIY   +VD+E   K   L
Sbjct:   18 EFSRDYSKQMFVKFRKR-VDEKLDILQFMEDSTSNFRFSKTPMFMQQIVNRKPTNIHNKPEFGLGSSYMKEK-----------HCKEIGNKSPAGHLLTIDKGQTQRKFKTFPQQMNTTCKYLLFVRKSESLFEAQPIENWYKM-----NKFTKLDFESIEEIEREQMKIEETANNLSARFLNKY-----------------DTEGKENKTETGNKNEEFSDS-ETIEKEDIRNLKKIAVFNRARYGKGNNYECESDVDDHQGDEVDYMSDSSCDEEILSNDEREKIYEAGHVDQEPVLKDRNL 286
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Planmine SMEST
Match: SMESG000056086.1 (SMESG000056086.1)

HSP 1 Score: 120.553 bits (301), Expect = 3.107e-28
Identity = 95/298 (31.88%), Postives = 160/298 (53.69%), Query Frame = 3 
Query:  279 DHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQKIKTSQG-PIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPIN--LSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGKERYYEESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEAN 1163
            ++E+ K ++K++F+KF +   ++++DILQF +D +   + + K MFLQ+   ++   + G P FG G+ F ++ +             + E+  P    ++I +    KK+K   + M  NSQY VFIR S   FEA  +++WYK+ P        +E+S  +  +  +  +S+S  F+++Y                  EE +V H   +   N D+++ SD+   E++ + K+KK+     K     + +  ESDVDDHQGDEVDYM+ SS DED L + EKEK+Y   +VD+EA+
Sbjct:   28 NNEYTKDHAKQLFVKF-TKPTDRQLDILQFMDDSTSNYSFSEKPMFLQQIINKRGPRNNGEPEFGMGSSFKRQQK-----------HTRVEADVPTTYTITIDKANAQKKFKTAPQEMNPNSQYLVFIRKSESLFEAYPLENWYKINPFTKLDINSIEESERDQLRKGQIFDSSSKKFTEKYKV----------------EEKEV-HKNDIEFGNEDKEL-SDSEIQEKQILNKRKKDTVFQRKRHGQGDAHAGESDVDDHQGDEVDYMSDSSCDEDVLSDGEKEKLYEAVDVDQEAD 295
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Planmine SMEST
Match: SMESG000055485.1 (SMESG000055485.1)

HSP 1 Score: 114.39 bits (285), Expect = 5.084e-27
Identity = 99/295 (33.56%), Postives = 147/295 (49.83%), Query Frame = 3 
Query:  312 IFIKFRSSECEKRIDILQFNNDGSLEQNLNSKEMFLQRDRTQK-IKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDPIN--LSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQPEVISRFPKLE-DSIEEYNKVAKKTESTSIIFSKRYLTXXXXXXXXXXXXRNSDEESDVYHGPGLIITNNDQDIFSDTTDDXXXXXXXXXXNAALLSKIRLGKERYYE-ESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEANSKSAQL 1181
            +F+KFR    ++++DILQF  D +     +   MF+Q+   +K       P FG G+ + KE            + K+  +K P    L+I +G   +K+K   + M    +Y +F+R S   FEA  +++WYKM     ++F KL+ +SIEE  +   K E T+   S R+L                  + D         T N  + FSD+ +  EKE ++  K  A+ ++ R GK   YE ESDVDDHQGDEVDYM+ SS DE+ L   E+EKIY   +VD+E   K   L
Sbjct:    1 MFVKFRKR-VDEKLDILQFMEDSTSNFRFSKTPMFMQQIVNRKPTNIHNKPEFGLGSSYMKEK-----------HCKEIGNKTPAGHLLTIDKGPTQRKFKTFPQQMNTTCKYLLFVRKSESLFEAQPIENWYKM-----NKFTKLDFESIEEIEREQMKIEETANNLSARFLN-----------------KYDTEGKENKTETGNKNEEFSDS-ETIEKEDIRNLKKIAVFNRARYGKGNNYECESDVDDHQGDEVDYMSDSSCDEEILSNDEREKIYEAGHVDQEPVLKDRNL 260
[Close]
BLAST of Transcription initiation factor IIF subunit alpha vs. Planmine SMEST
Match: SMESG000055520.1 (SMESG000055520.1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.004e-10
Identity = 36/66 (54.55%), Postives = 44/66 (66.67%), Query Frame = 3 
Query:  987 ALLSKIRLGKERYYE-ESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEANSKSAQL 1181
            A+ ++ R GK   YE ESDVDDHQGDEVDYM+ SS DE+ L   E+EKIY   +VD+E   K   L
Sbjct:   63 AVFNRARYGKGNNYECESDVDDHQGDEVDYMSDSSCDEEILSNDEREKIYEAGHVDQEPVLKDRNL 128
The following BLAST results are available for this feature:
BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GTF2F17.312e-827.61general transcription factor IIF subunit 1 [Source... [more]
GTF2F11.239e-727.61general transcription factor IIF subunit 1 [Source... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TfIIFalpha9.003e-628.93gene:FBgn0010282 transcript:FBtr0081719[more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
gtf2f12.535e-730.77general transcription factor IIF, polypeptide 1 [S... [more]
gtf2f12.535e-730.77general transcription factor IIF, polypeptide 1 [S... [more]
gtf2f13.661e-730.77general transcription factor IIF, polypeptide 1 [S... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gtf2f13.393e-925.75general transcription factor IIF subunit 1 [Source... [more]
gtf2f14.114e-925.75general transcription factor IIF subunit 1 [Source... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gtf2f13.601e-1025.65general transcription factor IIF, polypeptide 1 [S... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6AY96|T2FA_RAT2.371e-925.65General transcription factor IIF subunit 1 OS=Ratt... [more]
sp|Q3THK3|T2FA_MOUSE2.519e-925.65General transcription factor IIF subunit 1 OS=Mus ... [more]
sp|Q04870|T2FA_XENLA5.354e-825.60General transcription factor IIF subunit 1 OS=Xeno... [more]
sp|Q5EA53|T2FA_BOVIN2.881e-727.61General transcription factor IIF subunit 1 OS=Bos ... [more]
sp|P35269|T2FA_HUMAN3.511e-727.61General transcription factor IIF subunit 1 OS=Homo... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1EWJ26.131e-2433.17Uncharacterized protein OS=Helobdella robusta OX=6... [more]
A0A448X8F63.382e-1732.29Transcription initiation factor IIF subunit alpha ... [more]
A0A0F7GWW62.016e-1537.88General transcription factor IIF 1 (Fragment) OS=S... [more]
A0A095A2L01.278e-1332.74Dehydrogenase/reductase SDR family member 12 OS=Sc... [more]
A0A430Q7621.385e-1332.74Transcription initiation factor TFIIF subunit alph... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gtf2f11.229e-824.74general transcription factor IIF, polypeptide 1 [S... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gtf2f16.251e-928.46general transcription factor IIF, polypeptide 1 [S... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO487602.545e-731.93Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
gtf2f12.634e-725.60general transcription factor IIF subunit 1 [Source... [more]
gtf2f13.040e-725.60general transcription factor IIF subunit 1 [Source... [more]
gtf2f13.075e-725.60general transcription factor IIF subunit 1 [Source... [more]
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BLAST of Transcription initiation factor IIF subunit alpha vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000056085.13.022e-54100.00SMESG000056085.1[more]
SMESG000055295.12.184e-3034.21SMESG000055295.1[more]
SMESG000056086.13.107e-2831.88SMESG000056086.1[more]
SMESG000055485.15.084e-2733.56SMESG000055485.1[more]
SMESG000055520.11.004e-1054.55SMESG000055520.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007951 ID=SMED30007951|Name=Transcription initiation factor IIF subunit alpha|organism=Schmidtea mediterranea sexual|type=transcript|length=4280bp
CAGTTTTGAAAATAGTATAAATAAATAATATTTTGAAAATTTAAATGTTA
TTTGAAGTTGATTGATCGACATTCAAGAAAAATTTTTAAAATGAATAAGT
TATAAAATAGATGAATATTAATATACCTATATTATTGGCTAATTCCAATA
ATAAAGTTATTATAACATCCTCAAATGTTCCTAACAAACCAACTGCCAAT
TTGAGACTTATACAGAATTCAAGTTCAACACCTACTAATGGTTCACCTCA
AATTTCAACTAATCAGGTGTCCAATAAAGATCATGAATTTTTTAAAAAAT
ATTCCAAAGAAATATTTATTAAGTTTCGCAGTTCAGAATGTGAAAAACGA
ATTGATATCTTACAATTTAATAATGATGGCAGTTTAGAGCAAAATTTAAA
TTCTAAAGAAATGTTTTTGCAAAGGGATAGAACACAAAAAATCAAAACGA
GCCAAGGACCTATTTTTGGCGCCGGAACAGAATTTTGTAAAGAGGCTCGT
GATGAGGCATGGCAACGCAAATTTGCACTCTACAAAAAGAAAAGTGAATC
AAAGGATCCCATCAATCTGTCAATTGGACGGGGAAGAAATGTTAAAAAAT
ATAAGGCTGTTAAAGAAAGCATGTTTGAAAATTCACAGTATTGTGTTTTC
ATTCGCAGCAGTAATGATTGTTTTGAAGCAGTGTCAGTTAAGGATTGGTA
TAAAATGCAACCGGAAGTCATTTCACGATTTCCGAAATTAGAAGATTCCA
TAGAAGAGTATAATAAGGTAGCGAAAAAAACCGAATCTACTTCGATAATA
TTTTCAAAACGCTATCTCACGGAGGTTGATGAAGTAGTGAAGGCAAAGAA
AGTCAAAAGAAATTCGGATGAAGAATCAGATGTTTATCATGGGCCAGGAT
TAATTATTACTAATAATGATCAAGACATTTTCTCAGATACGACAGATGAC
GAAGAAAAAGAGGTGATGAAGAAAAAGAAAAATGCCGCCCTTCTGAGCAA
AATAAGATTGGGTAAAGAAAGATATTACGAAGAGAGTGATGTTGATGATC
ATCAAGGAGATGAGGTCGATTATATGACTCATTCGTCATCCGACGAGGAT
AATTTACCTGAAATGGAAAAGGAAAAAATTTATACAGTAAATAATGTTGA
TGAAGAAGCAAATAGTAAATCGGCTCAACTAGATCCAGTTGTTAGCATGG
AATATTTCGTCAGTAAATTGTACTTTCTATAAATCGGCTTCCTACTCAAC
AAGAAGTATTTAATTGTTTATTGTGGTATCGAAACCAATCCCACGAAGCT
TCATCACAAATTCAGTCTTTGCGAGATATTATGAAACTTGTAGCAGTAGA
TGTAATTTCCATCTGGGCGAATGCTAACATTCTTACCATAGCTCAGCATA
GTGTTATTAACAAACTAGAGAAGTTAGTAACACGAGGTCAAGAATTTGAA
AAATATGCTCTCGCTAGATAAAAAGCCTCTTCTTTTCAAGCAACTAATGA
ATCATTTCAGACAATTTTTTATATATGTTCTTGGAATACATTAGCAAAGG
AATCTCAGATAAAAACCAGTGCATTTCTAAAACGAAAGTTCCTAGAATTG
AGTGGGAATTTTGGGTGGACTAAATTTTAGAAAGAAATATGGTGATAGGA
AATGTAGATTATGATGCATTAACTGCTCTCGAAAGACAAACTCAAAGAAA
AGAGAAATGCCCGAACCTAAAGAAAGAAAGTACAAATAGCAAAAAACGGA
TATTCAGTCGAATAAAACCTTTCGCCACAAGAAACAGAATCAAGTGAAGG
CAATAAAGATTTATGGAATGCAAAACATTGGCTAAAGAATCAGGTAGATC
AGAGCCTGAGAGGTGTCGTGTCTACAATAAGTATCAATATCGTTCTCTTT
GTGAAATTATGGTACGAACCGGAATACGTAACCGAGACGCCAGTCAGGTA
ATTAATGCTTATTTACAATATATGAATATCAATCTAATAATGCCTTCAAA
ATTGCGAAGACAGCGTATTCTACATAGAACAGCAGCTGCTATTAGTCACT
GTATTTCCAATCAAAATTTACTATGCATCGGTTTTGATGGAGGAATTGAT
AAAACTAGGTTGTATGAAGGGAAAATAGCCAGAATTACAAAGAAAACCAC
TATGTAATAGTCTCATTTCTAGTTGGGCCTTATGTAGATCACGTATCAAG
GAAAAAACTAAGAAAATTAAGATATTGAAACTAAAGAATGATATTTCACA
AGTTGATTAGCTTTTCTCGTATTCAGTGTTCAACCTAACACTATCGCTAA
TGTTTGTATTGTGACGTTAAAGTAGTTTAAAAATATACATTGTGTAATTT
CACTATTTTCTTTTACGTAAATTCAAGAGCCCTAGACATTAACCAATTTC
AAAAAAAATTTGCATGTAAACTTTGGGTATACAAAGACAACTTTTTTTAG
TAGAGACAAAATAGTCCTGTAAGTTTTGTATTCCATCGGCCTGCCCTACC
GCACAAACTAAAGATAGATTATCGGTTGTTTCGAACCGTCCTCGAGATAT
TACTAAGATGGCGAAAGCTGTATCCTAATTAATCAGCAGTCCCGCTTATC
AAAGTTGGTGAACGACGATGGTTTCACAAATTTTATCCGCAATCGATTAA
CACCCAGAAATCGGTGATAATGTTATCCTATATAATCTACCCTCTGCTAC
TTTGAATAAAAATGTGACAACTGATGTCCACTATTCAAATCTTTCTAGAA
CTGTTACAACTGAAAGATCAGTCTTACGATCTGATGAACAACTTATGTAG
ATAAAGTAACGAAGAAATTCGCTATTTACCTTTTATAAGTTATTGGAACT
TTCTCTTTCTAACTTTCACTTTGATAGACCAAATCACTCGCTTAATTTCA
TTTTAAATTCTGCTGGGATTACGTATTTGTATCAAATCTAATTCGCTTTT
AAACTGCTGTAAGATCTCTTTCATAGATCTCAGTTTACACTTTCGAAATT
TCGGGTTTCTCAGATTAAAATGATTGGTTTACTTCTACAATATCTGTTTA
ATTGTCTAAATCCGTTAGTTGTCTCTTATCACAAAATTCAATCTCATTTT
ATACAGATTGAAAAATTAATTTATTTATTTTTGGTATTTAAAAATTATCA
TTTTTAAATTAAAATTGTATTATTTAAATACTAAAATTAAAATTTTTCCT
ATTTATAGTAATTTTAACTATTTTTAGTAATTAATTTGTAACTATAATTA
TTTATTTACAAATAACACAAAGGCAAGCACCAAACGAGCCCATGTCTAAC
CCTAACAAGTTTACATATTAAACATGTCCTAATTATTGTTTTAAAATACT
AACGACTGCATGTTCTGGAAGTGTCCGGTTACTTAAATCTATAATAAAAT
ATCCTTCTTATTGACCATACGTCACCGGAACACAATACTTTTCTTGTAGG
AATATCCATTTTGGGAATCATTTTCCCATTTTCTTTCAACAATTTCCAAT
TATTTTTAAGGCATTTTGTATTTTACATAGGAAAATTGATTTAAAATTCC
TGATATTCGAATTTCCTATTTTCGTTTATAATATTTATGATATTTTATAA
TATCTGTTTGCAAAGCTGATGTTCTAAATGTTTAAAACGCATATTTCCAA
GATTTAGCTCTGGCTGTACTTATTATATTTATTTTTTATTTATTCCAACT
TTTACTAAACCAGATGATGAATTATCCAATATGGACGAAGAAGTAAATGC
TAACGCAAATACAGAAGAAAGTGACTCTAAAACTATAAAAGAAATCGAAG
TTGAAAGCAATGCTGATTCTGACTCAAGTAATGATTCCAGTCCGATGTCG
TCCATAGATGAAAGTGACGATGAGGATAGCAACACGGCTCTCAAACAGAA
ATTAAGCACCACAATCAACAAGAATATCGCTAGTCAAATGAAATTGAATT
CTCGTTCTCAATCAAATAGCAATCTCTCTCATTTCGAAGATATATCTCGA
AAATTTAAGCAGGACATCTCTCAAAACAATCGTATCATTCAGGAAATGTA
CAAATATCTCAACAGGAAACCGATTTCTAAATCAAATCTATTCAAAAAGC
TCATTTTGAAAAAGTTGCTTTCTAATGAATCTCAGAAATTGTTCGACAGC
CTTCTCGACAAGATCAACACGATTACCCATATTGTTAATGGGGAAAAGCT
TTTCAGTATCATTAATTAAGGCGCGACATCGACTACTGTTTTTGTTTGCA
TTTACACTTTTATATTTTATTGATTGTTAG
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protein sequence of SMED30007951-orf-1

>SMED30007951-orf-1 ID=SMED30007951-orf-1|Name=SMED30007951-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MDEEVNANANTEESDSKTIKEIEVESNADSDSSNDSSPMSSIDESDDEDS
NTALKQKLSTTINKNIASQMKLNSRSQSNSNLSHFEDISRKFKQDISQNN
RIIQEMYKYLNRKPISKSNLFKKLILKKLLSNESQKLFDSLLDKINTITH
IVNGEKLFSIIN*
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protein sequence of SMED30007951-orf-2

>SMED30007951-orf-2 ID=SMED30007951-orf-2|Name=SMED30007951-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=374bp
MNINIPILLANSNNKVIITSSNVPNKPTANLRLIQNSSSTPTNGSPQIST
NQVSNKDHEFFKKYSKEIFIKFRSSECEKRIDILQFNNDGSLEQNLNSKE
MFLQRDRTQKIKTSQGPIFGAGTEFCKEARDEAWQRKFALYKKKSESKDP
INLSIGRGRNVKKYKAVKESMFENSQYCVFIRSSNDCFEAVSVKDWYKMQ
PEVISRFPKLEDSIEEYNKVAKKTESTSIIFSKRYLTEVDEVVKAKKVKR
NSDEESDVYHGPGLIITNNDQDIFSDTTDDEEKEVMKKKKNAALLSKIRL
GKERYYEESDVDDHQGDEVDYMTHSSSDEDNLPEMEKEKIYTVNNVDEEA
NSKSAQLDPVVSMEYFVSKLYFL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR008851TFIIF-alpha
IPR011039TFIIF_interaction
IPR011991WHTH_DNA-bd_dom
Vocabulary: biological process
TermDefinition
GO:0006367transcription initiation from RNA polymerase II promoter
GO:0032968positive regulation of transcription elongation from RNA polymerase II promoter
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0003824catalytic activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008851Transcription initiation factor IIF, alpha subunitPFAMPF05793TFIIF_alphacoord: 74..340
e-value: 3.3E-17
score: 62.3
IPR008851Transcription initiation factor IIF, alpha subunitPANTHERPTHR13011TFIIF-ALPHAcoord: 45..349
IPR011039Transcription Factor IIF, Rap30/Rap74, interactionSUPERFAMILYSSF50916Rap30/74 interaction domainscoord: 66..203