Granulin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007938 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of Granulin vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 170.244 bits (430), Expect = 9.426e-47 Identity = 115/401 (28.68%), Postives = 168/401 (41.90%), Query Frame = 1
Query: 220 TKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTR----NVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD----LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
C +C T + CC +P A+ C D +HCCP G C A + C + N G ++ P + P + T V D G++ CCP A CC D+ +CCP CDL + +C+ + K + N S CP++ +CP CC + G +GCC CC D CCP +T CD + C+SK + LT++P + + + CPD TCC + G +GCCPF A CC D+ CCP CD +K C+ P + K+ + L LK V C + + C + CC+ + + CC Y+ GVCCAD++ CCP G +C + G
Sbjct: 65 AHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGNNSVGAIQCPDSQFECP-----------------DFSTCCVMVD---------------------GSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSS------SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGY-------TCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARG 414 HSP 2 Score: 136.346 bits (342), Expect = 1.742e-34 Identity = 106/357 (29.69%), Postives = 159/357 (44.54%), Query Frame = 1
Query: 139 TDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQ 1182
DG F ++ + V CP+++ +C + TCCV S+ CC P A CC D+ HCCP G+ CD RC P + + LP + + + ++ + CP+ +++C D TCC G Y CCP NA CCSD +CCP++T CDL KCL S +NA + +L T V + KC + + CP CC + G +GCC F + +CC D CCP CD+ K C + K+ +P+ + + + +N+ C S +TCC + CCP++ CC+D CCP C C +E +
Sbjct: 107 ADGRSCFQRSGNNSVGAIQ---CPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT----PTGTHPLAKKLPA--------------QRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL-------SKENA---TTDLLTKLPAHTVGDV--KC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNV-----SSCPSSDTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPR 424 HSP 3 Score: 112.849 bits (281), Expect = 3.114e-26 Identity = 68/204 (33.33%), Postives = 89/204 (43.63%), Query Frame = 1
Query: 781 DKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL--KVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
D C C G CC F + + C D CCP C + ++C QR + I CPD EC F TCC + DG +GCCP A+CC D CCPH CDL C + P + K + L V+CPD +++C + S CC++ +YGCC N CC+D CCP T C
Sbjct: 64 DAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCF-------------QRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHP-LAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVC 253 HSP 4 Score: 95.5153 bits (236), Expect = 1.594e-20 Identity = 70/270 (25.93%), Postives = 115/270 (42.59%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQI 987
V+ + +CP+ R++C + TCC Y CC PNA CC D HCCP + CD + +C + +EN +L P+ T+ + + E + D TC ++ G + CCP AVCC D +CCP CD + C ++ ++ A + SL + L++ P ++ CP CC + + CC + +G+CC D + CCP C + C+ + + + P Q+
Sbjct: 200 VALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKC-----------LSKENATTDLLTKLPAHTVGDVKCDME-VSCPDGYTCCRLQS-------------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQ-----GPHQVPWMEKAPAH--LSLPDPQALKRDVPCDNVSSCPSSDTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPALRQL 437
BLAST of Granulin vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 171.014 bits (432), Expect = 7.310e-46 Identity = 116/401 (28.93%), Postives = 166/401 (41.40%), Query Frame = 1
Query: 220 TKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTR----NVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD----LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
C +C T + CC +P A+ C D +HCCP G C A + C + N G ++ P + P + T V D G++ CCP A CC D+ +CCP CDL + +C+ + K + N S CP++ +CP CC + G +GCC CC D CCP +T CD + C+SK + LT++P + + + CPD TCC + G +GCCPF A CC D+ CCP CD +K C+ P + K+ + L LK V C + + C + CCQ+ ++GCC VCC+D Q CCP G CV+ G
Sbjct: 65 AHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGNNSVGAIQCPDSQFECP-----------------DFSTCCVMVD---------------------GSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALS------SSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGY-------TCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEG 414 HSP 2 Score: 170.244 bits (430), Expect = 1.397e-45 Identity = 130/425 (30.59%), Postives = 189/425 (44.47%), Query Frame = 1
Query: 139 TDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKE--TSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
DG F ++ + V CP+++ +C + TCCV S+ CC P A CC D+ HCCP G+ CD RC P + + LP + + + ++ + CP+ +++C D TCC G Y CCP NA CCSD +CCP++T CDL KCL S +NA + +L T V + KC + + CP CC + G +GCC F + +CC D CCP CD+ K C + K+ +P+ + + + +N+ C S +TCC + G +GCCP A CCSD+ CCP C E + + E + ++ L +IG C T C CC + CC + VCC D+Q CCP G C
Sbjct: 107 ADGRSCFQRSGNNSVGAIQ---CPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT----PTGTHPLAKKLPA--------------QRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL-------SKENA---TTDLLTKLPAHTVGDV--KC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIG----CDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTC 488 HSP 3 Score: 167.162 bits (422), Expect = 1.563e-44 Identity = 115/407 (28.26%), Postives = 185/407 (45.45%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK-LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK-VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
V+ + +CP+ R++C + TCC Y CC PNA CC D HCCP + CD + +C + +EN +L P+ T+ + + E + D TC ++ G + CCP AVCC D +CCP CD + C ++ ++ A + SL + L++ P ++ CP CC ++ G +GCC + +CC D + CCP C A+ C S + + R A + +I D++ C +TCC G + CC +A CC D CCP C+++ + C+ + S + + L +G+K V C + C ++ CC+ + + CC Y+ GVCCAD++ CCP G +C + G
Sbjct: 200 VALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKC-----------LSKENATTDLLTKLPAHTVGDVKCDME-VSCPDGYTCCRLQS-------------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQ-----GPHQVPWMEKAPAH--LSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVS----AQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARG 569 HSP 4 Score: 114.005 bits (284), Expect = 3.073e-26 Identity = 82/324 (25.31%), Postives = 133/324 (41.05%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQ 1182
+ C + TCC + ++ CC + A+CC D HCCP+G TCD K C +GP + P +E+ P + P + ++ + + D C G + CCP AVCCSD +CCP+ C + +C S + + + A+L + + D+ CP + CCP GG + CC+ +CC D + CCP C+ ++C K + R + + ++ + C +TCC + CCP++ CC+D CCP C C +E +
Sbjct: 285 DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCE---QGPHQVPWMEK-APAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTS--------------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASL-SHPRDIGCDQ--------HTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC-EKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPR 579 HSP 5 Score: 112.079 bits (279), Expect = 1.302e-25 Identity = 68/204 (33.33%), Postives = 89/204 (43.63%), Query Frame = 1
Query: 781 DKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL--KVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
D C C G CC F + + C D CCP C + ++C QR + I CPD EC F TCC + DG +GCCP A+CC D CCPH CDL C + P + K + L V+CPD +++C + S CC++ +YGCC N CC+D CCP T C
Sbjct: 64 DAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCF-------------QRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHP-LAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVC 253 HSP 6 Score: 75.0998 bits (183), Expect = 1.116e-13 Identity = 66/253 (26.09%), Postives = 106/253 (41.90%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVE--ENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL-LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP 960
+N + C + TCC + CC P A+CC D HCCP G TC A+ +C R E +V E +P + L + + S + +TCC + G++ CC +AVCC D+ +CCP C++K + C + SA TF + +V + +C C + CC + + CC + +G+CC D + CCP C + C+ + + + P
Sbjct: 367 DNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSE------IVAGLEKMPARRASLSHPRDIGCDQHTSCPV-----------------GQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC-----------EKEVVSAQPATFLARSPHVGVKDVEC-GEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAP 583
BLAST of Granulin vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 143.28 bits (360), Expect = 5.293e-37 Identity = 106/394 (26.90%), Postives = 167/394 (42.39%), Query Frame = 1
Query: 220 TKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK-LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK-VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
C +C T + CC +P A+ C D +HCCP G C A + C + N V + P S + ++ + V+ G++ CCP AVCC D +CCP CD + C + A A+L SL + L++ P ++ CP CC ++ G +GCC + +CC D + CCP C A+ C S + + R A + +I D++ C +TCC G + CC +A CC D CCP C+++ + C+ + S + + L +G+K V C + C ++ CC+ + + CC Y+ GVCCAD++ CCP G +C + G
Sbjct: 65 AHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQR----------SGNNSVGAIQC-PDSQFECPDFSTCCVMVD-----------------------GSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKA---PAHL----SLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVS----AQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARG 412 HSP 2 Score: 126.331 bits (316), Expect = 5.181e-31 Identity = 91/348 (26.15%), Postives = 145/348 (41.67%), Query Frame = 1
Query: 139 TDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQ 1182
DG F ++ + V CP+++ +C + TCCV S+ CC P A+CC D HCCP+G TCD K C +GP + P +E+ P + P + ++ + + D C G + CCP AVCCSD +CCP+ C + +C S + + + A+L + + D+ CP + CCP GG + CC+ +CC D + CCP C+ ++C K + R + + ++ + C +TCC + CCP++ CC+D CCP C C +E +
Sbjct: 107 ADGRSCFQRSGNNSVGAIQ---CPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCE---QGPHQVPWMEK-APAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTS--------------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASL-SHPRDIGCDQ--------HTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC-EKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPR 422 HSP 3 Score: 117.087 bits (292), Expect = 8.255e-28 Identity = 69/207 (33.33%), Postives = 91/207 (43.96%), Query Frame = 1
Query: 781 DKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
D C C G CC F + + C D CCP C + ++C QR + I CPD EC F TCC + DG +GCCP A CC D+ CCP CD +K C+ P + K+ + L LK V C + + C + CCQ+ ++GCC VCC+D Q CCP G CV+ G
Sbjct: 64 DAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCF-------------QRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEG 257 HSP 4 Score: 75.8702 bits (185), Expect = 4.623e-14 Identity = 66/253 (26.09%), Postives = 106/253 (41.90%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVE--ENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL-LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP 960
+N + C + TCC + CC P A+CC D HCCP G TC A+ +C R E +V E +P + L + + S + +TCC + G++ CC +AVCC D+ +CCP C++K + C + SA TF + +V + +C C + CC + + CC + +G+CC D + CCP C + C+ + + + P
Sbjct: 210 DNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSE------IVAGLEKMPARRASLSHPRDIGCDQHTSCPV-----------------GQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKE-----------VVSAQPATFLARSPHVGVKDVEC-GEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAP 426
BLAST of Granulin vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 140.584 bits (353), Expect = 1.994e-36 Identity = 103/386 (26.68%), Postives = 165/386 (42.75%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK-LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK-VICPDQTKCENDSVCCQMDDNQYGCCSYKN 1329
V+ + +CP+ R++C + TCC Y CC PNA CC D HCCP + CD + +C + +EN +L P+ T+ + + E + D TC ++ G + CCP AVCC D +CCP CD + C + A SL + L++ P ++ CP CC ++ G +GCC + +CC D + CCP C A+ C S + + R A + +I D++ C +TCC G + CC +A CC D CCP C+++ + C+ + S + + L +G+K V C + C ++ CC+ + + CC Y+
Sbjct: 14 VALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKC-----------LSKENATTDLLTKLPAHTVGDVKCDME-VSCPDGYTCCRLQS-------------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKA-------PAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVS----AQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQ 362 HSP 2 Score: 135.576 bits (340), Expect = 1.417e-34 Identity = 92/284 (32.39%), Postives = 128/284 (45.07%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKE--TSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
G Y CCP NA CCSD +CCP++T CDL KCL S +NA + +L T V + KC + + CP CC + G +GCC F + +CC D CCP CD+ K C + K+ +P+ + + + +N+ C S +TCC + G +GCCP A CCSD+ CCP C E + + E + ++ L +IG C T C CC + CC + VCC D+Q CCP G C
Sbjct: 41 GKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL-------SKENA---TTDLLTKLPAHTVGDV--KC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIG----CDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTC 302 HSP 3 Score: 131.339 bits (329), Expect = 4.484e-33 Identity = 74/214 (34.58%), Postives = 104/214 (48.60%), Query Frame = 1
Query: 769 CPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD----LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
CP++ +CP CC + G +GCC CC D CCP +T CD + C+SK + LT++P + + + CPD TCC + G +GCCPF A CC D+ CCP CD +K C+ P + K+ + L LK V C + + C + CCQ+ ++GCC VCC+D Q CCP G CV+ G
Sbjct: 22 CPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGY-------TCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEG 228 HSP 4 Score: 97.8265 bits (242), Expect = 2.138e-21 Identity = 73/295 (24.75%), Postives = 122/295 (41.36%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNAT 1095
+ C + TCC + ++ CC + A+CC D HCCP+G TCD K C +GP + P +E+ P + P + ++ + + D C G + CCP AVCCSD +CCP+ C + +C S + + + A+L + + D+ CP + CCP GG + CC+ +CC D + CCP C+ ++C K + R + + ++ + C +TCC + CCP++ +
Sbjct: 99 DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCE---QGPHQVPWMEK-APAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTS--------------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASL-SHPRDIGCDQ--------HTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC-EKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQVS 364 HSP 5 Score: 93.9745 bits (232), Expect = 3.950e-20 Identity = 51/144 (35.42%), Postives = 71/144 (49.31%), Query Frame = 1
Query: 952 EIPNERQ-REAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
++P +R R + + +CPD C TCC + G YGCCP NATCCSD+ CCP + VCDL + C +KE + + L + V C + C + CC++ +GCC + VCC D CCP G C
Sbjct: 4 KLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDL-----LTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTC 142
BLAST of Granulin vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 67.781 bits (164), Expect = 1.950e-12 Identity = 43/122 (35.25%), Postives = 53/122 (43.44%), Query Frame = 1
Query: 796 GQKKCCPMSGGHFGC-CKF---NKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDL 1146
G CCP C C+F + + C D CCP C + ++C QR + I CPD EC F TCC + DG +GCCP A+CC D CCPH CDL
Sbjct: 79 GTSSCCPFPEIKGPCQCRFLCVPQAVACGDGHHCCPRGFHCSADGRSCF-------------QRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDL 187 HSP 2 Score: 54.6842 bits (130), Expect = 4.770e-8 Identity = 27/74 (36.49%), Postives = 38/74 (51.35%), Query Frame = 1
Query: 139 TDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKR 360
DG F ++ + V CP+++ +C + TCCV S+ CC P A CC D+ HCCP G+ CD R
Sbjct: 121 ADGRSCFQRSGNNSVGAIQ---CPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTR 191 HSP 3 Score: 52.373 bits (124), Expect = 3.004e-7 Identity = 34/115 (29.57%), Postives = 43/115 (37.39%), Query Frame = 1
Query: 565 NYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCD 909
+ C P AV C D ++CCP C + C S N +CP+S +CP CC M G +GCC + CC D CCPH CD
Sbjct: 96 RFLCVP--QAVACGDGHHCCPRGFHCSADGRSCFQRSG----------------------NNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCD 186 HSP 4 Score: 49.6766 bits (117), Expect = 2.956e-6 Identity = 37/137 (27.01%), Postives = 52/137 (37.96%), Query Frame = 1
Query: 1015 NVECQSFETCCSVGDGLYGCCPFK--------------NATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ C + +C G CCPF A C D CCP C + + C + + + +G + CPD Q +C + S CC M D +GCC CC D+ CCP+G C
Sbjct: 64 DAHCSAGHSCIFTVSGTSSCCPFPEIKGPCQCRFLCVPQAVACGDGHHCCPRGFHCSADGRSCFQRSGN--------------NSVG-AIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFC 185
BLAST of Granulin vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 97.0561 bits (240), Expect = 7.800e-22 Identity = 53/133 (39.85%), Postives = 67/133 (50.38%), Query Frame = 1
Query: 1009 DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL-KVICPDQ-TKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSK 1401
D EC ETCC +GD +GCCP NA CC D + CCP CD + C + + K K +V+CPD+ +KC + S CC ++ YGCC N VCCAD CCPNG C CS+
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQ 231 HSP 2 Score: 80.4925 bits (197), Expect = 2.841e-16 Identity = 48/173 (27.75%), Postives = 78/173 (45.09%), Query Frame = 1
Query: 706 LKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNN------ICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKS 1206
+K L + + + ++ +++ +C + CC + +GCC +CC D CCP T CD C+ +D +P ++++ A+ + +CPDK +C TCC + G YGCCP NA CC+D CCP+ C + C P + K S
Sbjct: 76 IKIMRLILLFLAVFLCRVTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIG--ADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC--HGQFCSQNFAMIPHLRKFAS 244 HSP 3 Score: 69.707 bits (169), Expect = 9.502e-13 Identity = 44/138 (31.88%), Postives = 63/138 (45.65%), Query Frame = 1
Query: 565 NYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCL--DSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQ 972
+ CCP NAVCC D+ +CCP T CD + +C+ D V K K+ F ++ CP+ +CP CC + G +GCC +CC DM CCP+ C + C S + IP+ R+
Sbjct: 117 TWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVV--------CPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHG--QFC---SQNFAMIPHLRK 241 HSP 4 Score: 67.781 bits (164), Expect = 3.765e-12 Identity = 53/144 (36.81%), Postives = 77/144 (53.47%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKC 642
+ T+C + +TCC N++ CC PNA+CC D+ HCCP+G+TCD RC E+++P+K K E N +++ CP+ +KC D TCC+ + G+Y CCP NAVCC+D +CCP C
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIG---------ADEKHVPMK----KKKPARKTVERNQ-----FNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 5 Score: 61.6178 bits (148), Expect = 4.549e-10 Identity = 27/52 (51.92%), Postives = 35/52 (67.31%), Query Frame = 1
Query: 187 EKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTC 342
+ NE VCP+ +KC + TCC+ + SY CC PNA+CC D HCCP+G TC
Sbjct: 173 QFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 6 Score: 55.4546 bits (132), Expect = 3.772e-8 Identity = 28/72 (38.89%), Postives = 39/72 (54.17%), Query Frame = 1
Query: 1186 SITKSKSLLHFYSEIGLKVI---CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
SI + +L F + +V C +T+C +D CC++ DN +GCC N VCC D+ CCP GT C G
Sbjct: 75 SIKIMRLILLFLAVFLCRVTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQG 146
BLAST of Granulin vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 96.6709 bits (239), Expect = 1.115e-21 Identity = 53/133 (39.85%), Postives = 67/133 (50.38%), Query Frame = 1
Query: 1009 DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL-KVICPDQ-TKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSK 1401
D EC ETCC +GD +GCCP NA CC D + CCP CD + C + + K K +V+CPD+ +KC + S CC ++ YGCC N VCCAD CCPNG C CS+
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQ 231 HSP 2 Score: 90.8929 bits (224), Expect = 1.023e-19 Identity = 70/264 (26.52%), Postives = 109/264 (41.29%), Query Frame = 1
Query: 706 LKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNN------ICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCP-----HNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEI------------------------GLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
+K L + + + ++ +++ +C + CC + +GCC +CC D CCP T CD C+ +D +P ++++ A+ + +CPDK +C TCC + G YGCCP NA CC+D CCP H + C + + + K + F SE G+ CP +T C N V + + + CC N +CC + TCCP G CV GG
Sbjct: 76 IKIMRLILLFLAVFLCRVTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIG--ADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVGKTCPAKTTCCN--VRGKNGEPKTMCCPLSNAICC--ENTCCPAGYHCVDGG 333 HSP 3 Score: 74.3294 bits (181), Expect = 3.512e-14 Identity = 63/223 (28.25%), Postives = 95/223 (42.60%), Query Frame = 1
Query: 565 NYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCL--DSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQ-------REAQIKL--------------NNICPDKNVE---------CQSFETCCSV----GDGLYGCCPFKNATCCSDNASCCP 1125
+ CCP NAVCC D+ +CCP T CD + +C+ D V K K+ F ++ CP+ +CP CC + G +GCC +CC DM CCP+ C + C S + IP+ R+ R+ ++ ++ P+ +V+ C + TCC+V G+ CCP NA CC + +CCP
Sbjct: 117 TWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVV--------CPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHG--QFC---SQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVGKTCPAKTTCCNVRGKNGEPKTMCCPLSNAICCEN--TCCP 324 HSP 4 Score: 73.1738 bits (178), Expect = 9.276e-14 Identity = 77/258 (29.84%), Postives = 114/258 (44.19%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLL--------NVDKLEKKCPNSD---------KQCPGQKKCCPMSGGH----FGCCKFNKGICCPDMKTCCPHNTDCDSAKK 921
+ T+C + +TCC N++ CC PNA+CC D+ HCCP+G+TCD RC E+++P+K K E N +++ CP+ +KC D TCC+ + G+Y CCP NAVCC+D +CCP C + C + A+ + S + L + + + P SD K CP + CC + G + CC + ICC + TCCP C K
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIG---------ADEKHVPMK----KKKPARKTVERNQ-----FNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC--HGQFCSQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVGKTCPAKTTCCNVRGKNGEPKTMCCPLSNAICCEN--TCCPAGYHCVDGGK 334 HSP 5 Score: 60.8474 bits (146), Expect = 7.745e-10 Identity = 27/50 (54.00%), Postives = 34/50 (68.00%), Query Frame = 1
Query: 193 NENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTC 342
NE VCP+ +KC + TCC+ + SY CC PNA+CC D HCCP+G TC
Sbjct: 175 NEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224
BLAST of Granulin vs. Ensembl Celegans
Match: T02B11.8 (pep chromosome:WBcel235:V:877530:878667:-1 gene:WBGene00044776.1 transcript:T02B11.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T02B11.8) HSP 1 Score: 45.4394 bits (106), Expect = 5.080e-6 Identity = 19/45 (42.22%), Postives = 26/45 (57.78%), Query Frame = 1
Query: 1258 TKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
T+C+ +VCC + + QYGCC + G CC CCP G C + G
Sbjct: 32 TQCDEYAVCCPIGNEQYGCCPFTGGTCCPGTNHCCPPGFSCTTIG 76
BLAST of Granulin vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 178.333 bits (451), Expect = 1.252e-47 Identity = 129/401 (32.17%), Postives = 176/401 (43.89%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSY-KNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT---EIPNERQREAQIKLNNICP-------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + K + S S + ND C D TCC TK G++ CCP AVCC D +CCP+ KC++ KC D K ++S T +++ K + C N CP CC G + CC + +CC D CCPH CD A +C S + ++P R+ ++ + + P + V C TCC DG +GCCP A CC D CCPH K C++ C + S P + K L KV C C S CC+ ++GCC + VCC D + CCP +C
Sbjct: 292 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDPSGSVSW---VEKVPVRPI-RKQKVAVTQVSSVSSDVPCND-------TAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQST---TTPNVIGTQKSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKC 676 HSP 2 Score: 173.326 bits (438), Expect = 6.053e-46 Identity = 128/411 (31.14%), Postives = 179/411 (43.55%), Query Frame = 1
Query: 160 NKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC--VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
K + QVS + +V N+ C + TCC TK + CC P A+CC D HCCP G C+ A +C E P+ E VK ++ ++T + + + ND C D TCC TK G + CCP AVCC D +CCP KCD+ C D S + ++ ++ V + N CP CC G +GCC + +CC D CCPH C+ A +C S S E R Q + + C TCC G +GCCPF A CC+D CCP + C+L C + P K + ++ L V+ C +Q+ C DS CC + ++ GCC + VCC D++ CCP G +C
Sbjct: 363 QKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--------EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-------TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKV-AVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRC 757 HSP 3 Score: 162.155 bits (409), Expect = 3.634e-42 Identity = 126/413 (30.51%), Postives = 177/413 (42.86%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL---TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSE------IGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
+ + +V N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + K + S + ND + CP+ T CKD G + CCP AVCC D +CCP KC++ C D S ++ ++ K+ KC N+ CP CC G +GCC F++ +CC D + CCP + C+ + +C+ + +I E ++ L + D+ C + TCC + GCCPF A CC D CCP CDL ++ C IT+ L H S + V C C + CC +GCC VCC D Q CCP G +C GG
Sbjct: 455 IGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDPSGSVPW---VEKVPVRPI-RKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDK-------DGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSV-----PWVEKVPVHLRAGQRPSGKV--KC-NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQ---LTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCGPGG 844 HSP 4 Score: 158.688 bits (400), Expect = 6.520e-41 Identity = 130/436 (29.82%), Postives = 183/436 (41.97%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC--TRNVEGPM----------EWPVVEENLP-VKMLDLKPSSTLHGFESN-------SETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ---------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+C N ++C TCC + CC P A+CC DK HCCP + CD +C + N E PM EW ++ P + +P+ T + +E V+ D+ N+ C D TCC TK G + CCP AVCC D +CCP KCD+ C D S + + ++ ++ V + P N C CC G + CC + +CC D CCP C+ A C + TE P +Q Q + +++ + C TCC DG + CCP A CC D CCPH K CD+ C++ S P I K K + S + + C D C + S CC+ D ++GCC VCC D CCP+G +C V+ G
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDV-ACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKV-AVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAG 601 HSP 5 Score: 129.413 bits (324), Expect = 3.444e-31 Identity = 113/441 (25.62%), Postives = 157/441 (35.60%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD------------LTEIPNERQREAQIKLNNICP-----------------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CPNN C+ Q+CC + CC + + CC D HCCP G C CT P+ + L K DL S + SE+ D + C+ +Y CCP + CSD +CCP + +C + C+ + + T C N +CP CC G +GCC K +CC D CCP +T CD CVS ++ E + +Q+ + + P + C TCC DG + CCP A CC D CCPH K CD+ C++ S I K K + S + V C D C + + CC+ + + CC VCC D CCP G +C
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATH---TQPLADRTL-AKKPDLPKSFRMIFSMPASESDITCPD-------GSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK-------------RKVKVQTVL-----------CGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKC 423 HSP 6 Score: 116.701 bits (291), Expect = 5.611e-27 Identity = 85/345 (24.64%), Postives = 136/345 (39.42%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL---LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-------------------------------KNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
G + CCP + CC D +CCPE C +K+ C +++ K +L + + + + + CP+ CP + C MS +GCC +G+ C D K CCP++ +C + CV + + + +C + EC + TCC DG +GCCP A CC D CCP + VCD++ C + T++P + + +L + + V C D C + S CC+ D + CC VCC D CCP+G +C +G C S+ ++ + K
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDG-SSCPAEFSCLLMSTS-YGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQTV--------------LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKV 365 HSP 7 Score: 83.5741 bits (205), Expect = 2.037e-16 Identity = 72/256 (28.12%), Postives = 101/256 (39.45%), Query Frame = 1
Query: 766 KCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC-------------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQT-KCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILYSTFEFTKFHECPLFLVA 1491
+CPN++ C + CC G F CC F+ G CC D CCP C C ++K DL + + + CPD + F C + YGCCP CSD CCP++ C + +C ++ + V+C + T +C D+ CCQ DD Q+GCC VCC DK CCP T C +K LK + + E + + P L A
Sbjct: 18 QCPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFS--CLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQT-----------------VLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVC----------DVKALKCVSSTNQELPMWDKFPARLRA 243
BLAST of Granulin vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 177.948 bits (450), Expect = 2.237e-47 Identity = 136/439 (30.98%), Postives = 187/439 (42.60%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSY-KNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT---EIPNERQREAQIKLNNICP-------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + K + S S + ND C D TCC TK G++ CCP AVCC D +CCP+ KC++ KC D K ++S T +++ K + C N CP CC G + CC + +CC D CCPH CD A +C S + ++P R+ ++ + + P + V C TCC DG +GCCP A CC D CCPH K C++ C + S P I K K + S V C D C + S CC+ D + CC VCC D CCP+G +C +G C S+ ++ + K
Sbjct: 292 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDPSGSVSW---VEKVPVRPI-RKQKVAVTQVSSVSSDVPCND-------TAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQST---TTPNVIGTQKSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKV 714 HSP 2 Score: 176.022 bits (445), Expect = 9.751e-47 Identity = 134/431 (31.09%), Postives = 184/431 (42.69%), Query Frame = 1
Query: 160 NKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPD----KNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
K + QVS + +V N+ C + TCC TK + CC P A+CC D HCCP G C+ A +C E P+ E VK ++ ++T + + + ND C D TCC TK G + CCP AVCC D +CCP KCD+ C D S + ++ ++ V + N CP CC G +GCC + +CC D CCPH C+ A +C S + + P ++Q+ A + ++ D C TCC DG + CCP A CC D CCPH K CD+ C++ S P I K K S + V C D C + S CC+ D +GCC VCC D CCP+G +C V+ G
Sbjct: 363 QKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--------EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-------TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKV-AVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAG 777 HSP 3 Score: 174.866 bits (442), Expect = 3.145e-46 Identity = 125/401 (31.17%), Postives = 168/401 (41.90%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPD----KNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + K + S + ND + CP+ T CKD G + CCP AVCC D +CCP KC++ C D S ++ ++ P N CP CC G + CC + +CC D CCPH CD A +C S + + P +Q+ A K++ + D V C TCC DG +GCCP A CC D CCPH K C++ C + S P + K L KV C C S CC+ ++GCC + VCC D + CCP +C
Sbjct: 465 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDPSGSVPW---VEKVPVRPI-RKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDK-------DGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKV-AVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKC 852 HSP 4 Score: 168.318 bits (425), Expect = 4.667e-44 Identity = 124/410 (30.24%), Postives = 177/410 (43.17%), Query Frame = 1
Query: 160 NKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC--VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
K + VS + ++ N+ C + TCC K + CC P A+CC D HCCP G C+ A C + G + W + +PV+ + + + ++S+ + ND C D TCC TK G + CCP AVCC D +CCP KCD+ C DSS + ++ + N CP CC G +GCC + +CC D CCPH C+ A +C S S E R Q + + C TCC G +GCCPF A CC+D CCP + C+L C + P K + ++ L V+ C +Q+ C DS CC + ++ GCC + VCC D++ CCP G +C
Sbjct: 536 QKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSC-DDPSGSVPW---MDKVPVRPIKKQKVAVTQASSASSD-VPCND-------TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRC 933 HSP 5 Score: 164.081 bits (414), Expect = 1.335e-42 Identity = 124/420 (29.52%), Postives = 180/420 (42.86%), Query Frame = 1
Query: 160 NKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT---EIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSE------IGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
K + Q S + +V N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + + + N++ V+ D+ C + + C G++ CCP AVCC D +CCP KC++ C D S ++ ++ K+ KC N+ CP CC G +GCC F++ +CC D + CCP + C+ + +C+ + +I E ++ L + D+ C + TCC + GCCPF A CC D CCP CDL ++ C IT+ L H S + V C C + CC +GCC VCC D Q CCP G +C GG
Sbjct: 624 QKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDSSGSVPW---VEKVPVRPIRKQKVA-------NTKVSPVSSDVACNDTVACPDGSTCCKDK-DGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSV-----PWVEKVPVHLRAGQRPSGKV--KC-NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQ---LTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCGPGG 1020 HSP 6 Score: 158.303 bits (399), Expect = 1.122e-40 Identity = 132/461 (28.63%), Postives = 189/461 (41.00%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC--TRNVEGPM----------EWPVVEENLP-VKMLDLKPSSTLHGFESN-------SETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ---------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
+C N ++C TCC + CC P A+CC DK HCCP + CD +C + N E PM EW ++ P + +P+ T + +E V+ D+ N+ C D TCC TK G + CCP AVCC D +CCP KCD+ C D S + + ++ ++ V + P N C CC G + CC + +CC D CCP C+ A C + TE P +Q Q + +++ + C TCC DG + CCP A CC D CCPH K CD+ C++ S P I K K + S + + C D C + S CC+ D ++GCC VCC D CCP+G +C +G C S+ + + + K
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDV-ACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKV-AVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKV 626 HSP 7 Score: 128.642 bits (322), Expect = 6.443e-31 Identity = 113/441 (25.62%), Postives = 157/441 (35.60%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD------------LTEIPNERQREAQIKLNNICP-----------------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CPNN C+ Q+CC + CC + + CC D HCCP G C CT P+ + L K DL S + SE+ D + C+ +Y CCP + CSD +CCP + +C + C+ + + T C N +CP CC G +GCC K +CC D CCP +T CD CVS ++ E + +Q+ + + P + C TCC DG + CCP A CC D CCPH K CD+ C++ S I K K + S + V C D C + + CC+ + + CC VCC D CCP G +C
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATH---TQPLADRTL-AKKPDLPKSFRMIFSMPASESDITCPD-------GSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK-------------RKVKVQTVL-----------CGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKC 423 HSP 8 Score: 115.931 bits (289), Expect = 9.114e-27 Identity = 85/345 (24.64%), Postives = 136/345 (39.42%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL---LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-------------------------------KNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
G + CCP + CC D +CCPE C +K+ C +++ K +L + + + + + CP+ CP + C MS +GCC +G+ C D K CCP++ +C + CV + + + +C + EC + TCC DG +GCCP A CC D CCP + VCD++ C + T++P + + +L + + V C D C + S CC+ D + CC VCC D CCP+G +C +G C S+ ++ + K
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDG-SSCPAEFSCLLMSTS-YGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQTV--------------LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKV 365 HSP 9 Score: 83.1889 bits (204), Expect = 3.483e-16 Identity = 72/256 (28.12%), Postives = 101/256 (39.45%), Query Frame = 1
Query: 766 KCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC-------------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQT-KCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILYSTFEFTKFHECPLFLVA 1491
+CPN++ C + CC G F CC F+ G CC D CCP C C ++K DL + + + CPD + F C + YGCCP CSD CCP++ C + +C ++ + V+C + T +C D+ CCQ DD Q+GCC VCC DK CCP T C +K LK + + E + + P L A
Sbjct: 18 QCPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFS--CLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQT-----------------VLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVC----------DVKALKCVSSTNQELPMWDKFPARLRA 243
BLAST of Granulin vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 172.94 bits (437), Expect = 9.550e-46 Identity = 127/411 (30.90%), Postives = 177/411 (43.07%), Query Frame = 1
Query: 160 NKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC--VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
K + QVS + +V N+ C + TCC TK + CC P A+CC D HCCP G C+ A +C E P+ E VK ++ ++T + + + ND C D TCC TK G + CCP AVCC D +CCP KCD+ C D S + ++ ++ P N CP CC G + CC + +CC D CCPH C+ A +C S S E R Q + + C TCC G +GCCPF A CC+D CCP + C+L C + P K + ++ L V+ C +Q+ C DS CC + ++ GCC + VCC D++ CCP G +C
Sbjct: 363 QKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--------EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-------TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKV-AVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRC 757 HSP 2 Score: 172.555 bits (436), Expect = 1.177e-45 Identity = 128/401 (31.92%), Postives = 172/401 (42.89%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSY-KNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPD----KNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + K + S S + ND C D TCC TK G++ CCP AVCC D +CCP+ KC++ KC D K ++S T +++ K + C N CP CC G + CC + +CC D CCPH CD A +C S + + P +Q+ A ++ D C TCC DG + CCP A CC D CCPH K C++ C + S P + K L KV C C S CC+ ++GCC + VCC D + CCP +C
Sbjct: 292 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDPSGSVSW---VEKVPVRPI-RKQKVAVTQVSSVSSDVPCND-------TAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQST---TTPNVIGTQKSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKC 676 HSP 3 Score: 162.54 bits (410), Expect = 3.127e-42 Identity = 128/413 (30.99%), Postives = 177/413 (42.86%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT---EIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSE------IGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
+ + +V N+ C + TCC TK + CC P A+CC D HCCP G CD A C + G + W E +PV+ + K + S S + ND C D TCC TK G + CCP AVCC D +CCP KC++ C D S ++ ++ K+ KC N+ CP CC G +GCC F++ +CC D + CCP + C+ + +C+ + +I E ++ L + D+ C + TCC + GCCPF A CC D CCP CDL ++ C IT+ L H S + V C C + CC +GCC VCC D Q CCP G +C GG
Sbjct: 455 IGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSC-EDPSGSVPW---VEKVPVRPI-RKQKVAVTTASSASSDVPCND-------TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSV-----PWVEKVPVHLRAGQRPSGKV--KC-NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQ---LTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCGPGG 844 HSP 4 Score: 154.066 bits (388), Expect = 2.101e-39 Identity = 130/436 (29.82%), Postives = 180/436 (41.28%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC--TRNVEGPM----------EWPVVEENLP-VKMLDLKPSSTLHGFESN-------SETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ---------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+C N ++C TCC + CC P A+CC DK HCCP + CD +C + N E PM EW ++ P + +P+ T + +E V+ D+ N+ C D TCC TK G + CCP AVCC D +CCP KCD+ C D S + + ++ ++ V + P N C CC G + CC + +CC D CCP C+ A C + TE P +Q Q + +++ + C TCC DG + CCP A CC D CCPH K CD+ C++ S P I K K + S V C D C + S CC+ D + CC VCC D CCP+G +C V+ G
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDV-ACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKV-AVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAG 601 HSP 5 Score: 129.028 bits (323), Expect = 3.985e-31 Identity = 113/441 (25.62%), Postives = 157/441 (35.60%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD------------LTEIPNERQREAQIKLNNICP-----------------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CPNN C+ Q+CC + CC + + CC D HCCP G C CT P+ + L K DL S + SE+ D + C+ +Y CCP + CSD +CCP + +C + C+ + + T C N +CP CC G +GCC K +CC D CCP +T CD CVS ++ E + +Q+ + + P + C TCC DG + CCP A CC D CCPH K CD+ C++ S I K K + S + V C D C + + CC+ + + CC VCC D CCP G +C
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATH---TQPLADRTL-AKKPDLPKSFRMIFSMPASESDITCPD-------GSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK-------------RKVKVQTVL-----------CGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKC 423 HSP 6 Score: 116.316 bits (290), Expect = 6.141e-27 Identity = 85/345 (24.64%), Postives = 136/345 (39.42%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL---LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-------------------------------KNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
G + CCP + CC D +CCPE C +K+ C +++ K +L + + + + + CP+ CP + C MS +GCC +G+ C D K CCP++ +C + CV + + + +C + EC + TCC DG +GCCP A CC D CCP + VCD++ C + T++P + + +L + + V C D C + S CC+ D + CC VCC D CCP+G +C +G C S+ ++ + K
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDG-SSCPAEFSCLLMSTS-YGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQTV--------------LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKV 365 HSP 7 Score: 83.5741 bits (205), Expect = 2.283e-16 Identity = 72/256 (28.12%), Postives = 101/256 (39.45%), Query Frame = 1
Query: 766 KCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC-------------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQT-KCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILYSTFEFTKFHECPLFLVA 1491
+CPN++ C + CC G F CC F+ G CC D CCP C C ++K DL + + + CPD + F C + YGCCP CSD CCP++ C + +C ++ + V+C + T +C D+ CCQ DD Q+GCC VCC DK CCP T C +K LK + + E + + P L A
Sbjct: 18 QCPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFS--CLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQT-----------------VLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVC----------DVKALKCVSSTNQELPMWDKFPARLRA 243
BLAST of Granulin vs. Ensembl Zebrafish
Match: grnb (granulin b [Source:ZFIN;Acc:ZDB-GENE-030131-7393]) HSP 1 Score: 168.318 bits (425), Expect = 1.408e-44 Identity = 123/409 (30.07%), Postives = 181/409 (44.25%), Query Frame = 1
Query: 178 QVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC--VSKSSDLTEIPNERQREA-QIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDD-NQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
+V+ E +CP+ +KC E TCC+ + SY CC P A+CC D+ HCCP G+TCD C + N +M P+ T+ + E + N+ + C + T CK G++ CCP AVCC D +CCPE T C++ C D + + S ++ + N KKC S CPG+ CC +S G +GCC + +CC D CCPH + C+ A + C VS S +P ++ A + D+ C + TCC + G + CCP A CC+D CCP CDL + C + + + L + + +C QT C D CC ++ ++GCC VCC +G C G
Sbjct: 179 EVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTC------------LSANGVSEMATKIPAVTV--LKPKEEVVPCNETVACSSGTTCCKTP-------EGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGL--SVSVPWMEKVSTKPIAPPPN-----KKCDES-SSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVKKIPAVSVPSQKQNCDETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEPALRETQRVEDRHMCDAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCK-------DGDHCCPSG 551 HSP 2 Score: 166.392 bits (420), Expect = 6.486e-44 Identity = 118/413 (28.57%), Postives = 180/413 (43.58%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSS-------AVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSK---SSDLTEIPNE---RQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-------DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+CP+ C++ TCC+T Y CC P+A CC D HCC G+ CD +C N ++W E + K+ + ++ ++ D G + CCP KNAVCC D+ +CCP+ T CDL + C+ ++ F++ + L+ + + N+ E CP+ +CP CC + G +GCC K +CC D K CCP T CD C+S S T+IP + +E + N + V C S TCC +G + CCP A CC D+ CCP +C++ C + S+ + ++ K I P + + C +S CC++ +GCC VCC D CCP+G+ C
Sbjct: 21 ICPDGGM-CEDGNTCCLTPSGGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCV-NKTHVLDW---VEKVEAKLQAVVCPDGESECPDDTTCCQMPD----------------------GGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIR--EVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSANGVSEMATKIPAVTVLKPKEEVVPCN-----ETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGLSVSVP-------WMEKVSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVC 392 HSP 3 Score: 166.392 bits (420), Expect = 7.007e-44 Identity = 121/400 (30.25%), Postives = 174/400 (43.50%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICP------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSK-SLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
VCP+ ++C + TCC + CC NA+CC D+ HCCP G+TCD C G + + K L+ K + L +N + D I+ T C +T G+Y CCP AVCCSD+ +CCPE T CDL + CL ++ V + A ++L + C N C CC G + CC K +CC D CCP T C+ A +C + +P + + I P D++ C TCC + G +GCCP A CC D+ CCPH VC++ + C+ S I+ + S K C + + C + CC++ + CC VCCAD++ CCP G C
Sbjct: 94 VCPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLE-KNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLET------GSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSANGVS--------EMATKIPAVTVLKPKEEVVPC-NETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGLSVSVPWMEK----VSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVKKIPAVSVPSQKQNCDETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTC 473 HSP 4 Score: 154.451 bits (389), Expect = 1.125e-39 Identity = 120/425 (28.24%), Postives = 188/425 (44.24%), Query Frame = 1
Query: 151 FSFNKASEDQVSEKNENVCPNNR----TKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFY-SLLNVDKLE-KKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLN--------NICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
S + ++VS K PN + + C TCC + CC P A+CC D HCCP GS C+ A + C + + + V M+ P+ ++ + N + + C TCC G++ CCP AVCC+D+ +CCP+ CDL C+ S GL S + +L ++E + ++ CP CC ++ G +GCC K +CC D CCP C+ K +C + L +IP ++ A++ + ++ D + C S TCC + G +GCCP A CC D+ CCP +C LE C E + ++ S + + ++ C T+C + CC++ D+ + CC Y VCC D + CCP G +C
Sbjct: 325 LSVSVPWMEKVSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGL-------RISVPMVKKIPAVSVPSQKQNCD------------ETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRS----------GLPSMAWFRKEPALRETQRVEDRHMCDAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSC---TKGLHQIPWFTKKTARVWKSSDELLGHEDVKCDSSTSCPSGSTCCILPTGQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTC---EKASADVSVSLTAVQMP-----EIQCDTFTRCAHTQSCCRLADSTWACCPYTQAVCCKDMKHCCPMGYKC 709 HSP 5 Score: 127.872 bits (320), Expect = 8.112e-31 Identity = 99/347 (28.53%), Postives = 141/347 (40.63%), Query Frame = 1
Query: 121 IIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC 1161
+ D L + K V + +N + + C TCC S+ CC P A+CC D+ HCCP G TCD A+ C R+ M W E L ET RV D C + +D C I + G + CCP AVCC D +CCP C+ + C + K+A ++ L + + KC +S CP CC + G +GCC K +CC D + CCP C C S+D++ +++ I D C ++CC + D + CCP+ A CC D CCP CD + + C
Sbjct: 402 VSDSGLRISVPMVKKIPAVSVPSQKQNC--DETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEPAL-------------------RETQRVEDRHMCDAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQIPWFTK---KTARVWKSSDEL-LGHEDVKC-DSSTSCPSGSTCCILPTGQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEKASADVS------VSLTAVQMPEIQCDTFTRCAHTQSCCRLADSTWACCPYTQAVCCKDMKHCCPMGYKCDPKVQGC 716
BLAST of Granulin vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 103.605 bits (257), Expect = 2.450e-24 Identity = 67/222 (30.18%), Postives = 97/222 (43.69%), Query Frame = 1
Query: 559 HGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSY-KNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPD----KNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSI 1191
G++ CCP AVCC D +CCP+ KC++ KC D K ++S T +++ K + C N CP CC G + CC + +CC D CCPH C+ A +C S + + P ++Q+ A + ++ D C TCC DG + CCP A CC D CCPH K C++ C + S P +
Sbjct: 62 EGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQS---TTTPNVIGTQKSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWV 279 HSP 2 Score: 102.449 bits (254), Expect = 6.895e-24 Identity = 79/267 (29.59%), Postives = 111/267 (41.57%), Query Frame = 1
Query: 160 NKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI 957
K + QVS + +V N+ C + TCC TK + CC P A+CC D HCCP G C+ A +C E P+ E VK ++ ++T + + + ND C D TCC TK G + CCP AVCC D +CCP KC++ C D S ++ ++ P N CP CC G + CC + +CC D CCPH C+ A +C S + +
Sbjct: 29 QKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--------EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-------TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKV-AVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWV 279 HSP 3 Score: 97.4413 bits (241), Expect = 3.566e-22 Identity = 74/269 (27.51%), Postives = 104/269 (38.66%), Query Frame = 1
Query: 643 DLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ---------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP--------SITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
D+ C D S + + ++ ++ V + P N C CC G + CC + +CC D CCP C+ A C + TE P +Q Q + +++ + C TCC DG + CCP A CC D CCPH K C++ C + S P I K K + S V C D C + S CC+ D + CC VCC D CCP+G +C V+ G
Sbjct: 2 DVAAGSCEDPSGSVSWVEKVPVRPIRKQKV-AVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC--EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAG 267
BLAST of Granulin vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 170.244 bits (430), Expect = 1.310e-44 Identity = 133/416 (31.97%), Postives = 182/416 (43.75%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCV-TKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLP--VKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ-IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
S ++ VCP+ R++C TCC+ + +S+ CC P A+CC D HCCP S CD + RC N + + W LP VK LK L G + D CP+ T C+ Y CC +AVCCSD +CCP T CDL +KKC+ + L + + + ++ CP + CC +S +GCC + + +CC D CCP C S IP R+ AQ +K + D C ETCC + G +GCCP A CC+D+ CCP C + C E S P TK+ +L H + V C D C + CC++ +GCC VCC D + CCP G C G C K + SI
Sbjct: 113 ASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDH-IPW---MSKLPARVKSEGLK----LVGLGDEERRVPCLDGTFCPDGSTCCEQVDH-------TYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTG-----------EGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCVLAEHS-----IPWMRKTLAQGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARD----VECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSI 491 HSP 2 Score: 143.28 bits (360), Expect = 1.276e-35 Identity = 120/414 (28.99%), Postives = 171/414 (41.30%), Query Frame = 1
Query: 181 VSEKNENVCP-NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL-TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
+ E++ N P + T C + TCC + CC Y A+CC D HCCPSG TC + E ++P L +T RV D + C + +TCC G + CCP AVCC+D +CCPE C ++ S + +T L + + +C + CP + CC ++ G +GCC + +CC D + CCP C + S L ++ P RQ +K D C +TCC + G +GCCP A CC D+ CCP C + C E S P +K+ +L + V C D C + CC++ +GCC VCC D + CCP G C G C K + SI
Sbjct: 277 IREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCV------------LAEHSIPWMRKTLA---------QGLKTTRVQCD-----DTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPW-------------FTKTPALTHEARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETKYVKC-----DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ--CMIGEHSIPWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSI 639 HSP 3 Score: 125.176 bits (313), Expect = 1.189e-29 Identity = 88/313 (28.12%), Postives = 129/313 (41.21%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPN--ERQREAQIKLNNI--------CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILYSTFEFTKFH 1467
G CCP C D ++CC + C C+ +S ++ CP+ +CP CC MS +GCC + +CC D CCPHN++CD + C+S + + R + +KL + C D C TCC D YGCC +A CCSD+ CCP CDL K C ++ P + + ++ E +V C T C + + CC + ++GCC Y VCC D CCP+G C G C + SI ++ L + T+
Sbjct: 77 GGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPAS-------------------------NQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTF-CPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAI---REESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCVLAEHSIPWMRKTLAQGLKTTRVQ 360 HSP 4 Score: 102.064 bits (253), Expect = 3.875e-22 Identity = 69/205 (33.66%), Postives = 93/205 (45.37%), Query Frame = 1
Query: 790 CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGD-GLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLL--HFYSEIGL-----KVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP + C G CC +G C D CC + C C IP Q +CPD EC + TCC + D +GCCP A CC D+ CCPHN CD+++ C + + P ++K + + +GL +V C D T C + S CC+ D+ YGCCS + VCC+D CCP GT C
Sbjct: 65 CPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC---------IPASNQSAV------VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTC 254 HSP 5 Score: 76.6406 bits (187), Expect = 5.524e-14 Identity = 116/461 (25.16%), Postives = 155/461 (33.62%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNK-----------------------------------------------GICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKN------------ATCCSDNA---SCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCS 1398
++ C + QTCC + CC A+CC D HCCP G TC ++ E + W L K D++ ++ R+ G++ CCP AVCC D +CCP C +C G S Y+ L + KC +S C CC M G +GCC K +CC D CCP CD A+ CV LT++P ++IC D + C S TCC G + CCP + AT C S CP + +K + ETS PS T + L Y V C Q C + CC+ + CC Y GVCC D CCP G C++ G S
Sbjct: 510 DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALKQKAKDVQ-CDDMYSCADGQTCCRLAS----------------------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC-----------QKGEHSIPWYSKTPALKQEGNIVKCDDS-FACKDGYTCCRMVSGVWGCCPIEKCCQTHTPLLPLQKCCPSLPILALATCHVQDSVQDSCTRLQSTHWCRTAVCCSDHWHCCPQGFTCD-ARGTCVLGQFSIPWLTKVPALPFD----GTHSICDDTHT-CPSGTTCCPGEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQK--EAIETS-PSDTSVQGLRLDY------VWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTS 915 HSP 6 Score: 53.1434 bits (126), Expect = 1.086e-6 Identity = 58/244 (23.77%), Postives = 88/244 (36.07%), Query Frame = 1
Query: 253 TKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCP-ENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL-------------LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS 942
T+ S H CR A+CC D +HCCP G TCDA V + L P+ G S + DD + CCP + + CCP E +K L ++A + + ++ +Y+L + +L+ +S C + CC GG + CC + +G+CCPDM CCP+ C + +C S
Sbjct: 721 TRLQSTHWCR--TAVCCSDHWHCCPQGFTCDARGT-------------CVLGQFSIPWLTKVPALPFDGTHS------ICDDTHTCPS----------------GTTCCPGEGG-----GWRCCPVEEVSKMVKRTVILLATACIVTLVSFSF-CPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGS 921 HSP 7 Score: 50.8322 bits (120), Expect = 4.936e-6 Identity = 29/92 (31.52%), Postives = 42/92 (45.65%), Query Frame = 1
Query: 118 IIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEW 393
III YAL + + + + V +++ C + QTCC ++CC Y +CCPD HCCP G C C+R+ G W
Sbjct: 835 IIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRS--GSPRW 924
BLAST of Granulin vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 164.466 bits (415), Expect = 9.765e-43 Identity = 131/425 (30.82%), Postives = 180/425 (42.35%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCV-TKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLP--VKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ-IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYS---------EIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
S ++ VCP+ R++C TCC+ + +S+ CC P A+CC D HCCP S CD + RC N + + W LP VK LK L G + D CP+ T C+ Y CC +AVCCSD +CCP T CDL +KKC+ + L + + + ++ CP + CC +S +GCC + + +CC D CCP C S IP R+ AQ +K + D C ETCC + G +GCCP A CC+D+ CCP C + C E S P TK+ + + V C D C + CC++ +GCC VCC D + CCP G C G C K + SI
Sbjct: 113 ASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDH-IPW---MSKLPARVKSEGLK----LVGLGDEERRVPCLDGTFCPDGSTCCEQVDH-------TYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTG-----------EGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCVLAEHS-----IPWMRKTLAQGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSI 504 HSP 2 Score: 137.117 bits (344), Expect = 1.649e-33 Identity = 126/439 (28.70%), Postives = 178/439 (40.55%), Query Frame = 1
Query: 181 VSEKNENVCP-NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKN-KKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL-TEIPNERQREAQIKLNN---------------ICP---------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
+ E++ N P + T C + TCC + CC Y A+CC D HCCPSG TC + E ++P L +T RV D + C + +TCC G + CCP AVCC+D +CCPE C K S F S +L + +++V + + +C + CP + CC ++ G +GCC + +CC D + CCP C + S L ++ P RQ A + ICP D C +TCC + G +GCCP A CC D+ CCP C + C E S P +K+ +L + V C D C + CC++ +GCC VCC D + CCP G C G C K + SI
Sbjct: 277 IREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCV------------LAEHSIPWMRKTLA---------QGLKTTRVQCD-----DTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVAR-DVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ--CMIGEHSIPWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSI 681 HSP 3 Score: 126.716 bits (317), Expect = 3.769e-30 Identity = 123/459 (26.80%), Postives = 191/459 (41.61%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKN-KKCLDSSAVFNSYKNAGLKSA-------NLYTFYSLLNVDKLEKKCPNS---------DKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS--DLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL------------LHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGGCSKVK*SIKCLKLILYSTFEFTK-----FHECP 1476
++ C E +TCC + CC A+CC D HCCP G TC ++ C++ +E + W P+ ++ S + + +D C D +TCC G++ CCP AVCC D +CCP C +K S +F+ ++A + Y F L CP S C + CC ++ G +GCC + +CC D + CCP C S + + + S ++ P +Q+ ++ +++ C +TCC + G +GCCP A CC D+ CCP C + C+ E S P +K+ +L ++ ++ G V C D C++ CC+M +GCC + VCC+D CCP G C G C + SI L + F+ T H CP
Sbjct: 362 DDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSK-MEHSIPW---FTKTPMVFFLSLSLLSILPVVSVARDVECDD-------MYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPF--ICPFSVAKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC-SGSQCMIGEHSIPWFSKTPALKQKAKDVQCDDM-----YSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQKGEHSIPWYSKTPALKQEGKCNLANTPVNVFA--GNIVKCDDSFACKDGYTCCRMVSGVWGCCPIEKAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCP 795 HSP 4 Score: 125.176 bits (313), Expect = 1.298e-29 Identity = 88/313 (28.12%), Postives = 129/313 (41.21%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPN--ERQREAQIKLNNI--------CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILYSTFEFTKFH 1467
G CCP C D ++CC + C C+ +S ++ CP+ +CP CC MS +GCC + +CC D CCPHN++CD + C+S + + R + +KL + C D C TCC D YGCC +A CCSD+ CCP CDL K C ++ P + + ++ E +V C T C + + CC + ++GCC Y VCC D CCP+G C G C + SI ++ L + T+
Sbjct: 77 GGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPAS-------------------------NQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTF-CPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAI---REESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCVLAEHSIPWMRKTLAQGLKTTRVQ 360 HSP 5 Score: 108.612 bits (270), Expect = 2.847e-24 Identity = 113/457 (24.73%), Postives = 168/457 (36.76%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAK-KRCTRNVEGPMEWPVVEENLPVKML---DLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICP-----------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL----LHFY---------------------------------------SEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCS 1398
++ C + QTCC + CC A+CC D HCCP G TC + ++ ++ + P + + D P F V + C + + C + G++ CCP AVCC D +CCP C +C+ G S ++ L + +C + C + CC ++ G +GCC + +CC D + CCP C + S ++E + L N P D + C+ TCC + G++GCCP + A CCSD+ CCP CD + C + S P +TK +L H S GL+ V C Q C + CC+ + CC Y GVCC D CCP G C++ G S
Sbjct: 449 DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLAS-GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCM-----------IGEHSIPWFSKTPALKQKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPWYSKTPALKQEGKCNLANT-PVNVFAGNIVKCDDSFACKDGYTCCRMVSGVWGCCPIEKAVCCSDHWHCCPQGFTCD-ARGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEEKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTS 888 HSP 6 Score: 102.064 bits (253), Expect = 4.587e-22 Identity = 69/205 (33.66%), Postives = 93/205 (45.37%), Query Frame = 1
Query: 790 CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGD-GLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLL--HFYSEIGL-----KVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP + C G CC +G C D CC + C C IP Q +CPD EC + TCC + D +GCCP A CC D+ CCPHN CD+++ C + + P ++K + + +GL +V C D T C + S CC+ D+ YGCCS + VCC+D CCP GT C
Sbjct: 65 CPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC---------IPASNQSAV------VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTC 254 HSP 7 Score: 77.7962 bits (190), Expect = 1.882e-14 Identity = 72/309 (23.30%), Postives = 122/309 (39.48%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAK-KRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQK---KCCPMS----------------------------------------GGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS 942
I F+K + +K ++V ++ C + QTCC + CC A+CC D HCCP G TC + ++ ++ + P +++ + + + ++ F N ++ +D C + T C+ G + CCP + AVCCSD ++CCP+ CD + + V + L F ++ CP+ CPG+ +CCP+ GG + CC + +G+CCPDM CCP+ C + +C S
Sbjct: 607 IPWFSKTPA--LKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPWYSKTPALKQEGKCNLAN----TPVNVFAGN--IVKCDDSFACKDGYTCCRMVS-------GVWGCCPIEKAVCCSDHWHCCPQGFTCDARG------TCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEEKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGS 894
BLAST of Granulin vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 147.132 bits (370), Expect = 5.880e-37 Identity = 120/431 (27.84%), Postives = 176/431 (40.84%), Query Frame = 1
Query: 214 NRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC-TRNVEGPM--EWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL-TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGGCSKVK*SIKCLKLILYSTFEFTK-----FHECP 1476
+ T C + TCC ++Y CC +A+CC D HCCP G+TCD K+C ++ EGP+ + P + E + + P E+ + G + CCP AVCC+D +CCPE C ++ S + +T L + + +C + CP + CC ++ G +GCC + +CC D + CCP C + S L ++ P RQ +K D C +TCC + G +GCCP A CC D+ CCP C + C E S P +K+ +L + V C D C + CC++ +GCC VCC+D CCP G C G C + SI L + F+ T H CP
Sbjct: 225 DGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIRE----ESANQVPCDATTKKETCCRLVS-------------------------GKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPW-------------FTKTPALTHEARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETKYVKC-----DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ--CMIGEHSIPWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCP 601 HSP 2 Score: 146.362 bits (368), Expect = 8.310e-37 Identity = 124/416 (29.81%), Postives = 180/416 (43.27%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCV-TKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKS-SDLTEIPNERQREAQIKLNNI--CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
S ++ VCP+ R++C TCC+ + +S+ CC P A+CC D HCCP S CD + RC N + + W LP ++ E RV + T C D TCC Y CC +AVCCSD +CCP T CDL +KKC+ + + L ++ + C + K+ + CC + G +GCC K +CC D CCP C + + + S T+ P ++ +++ CPD +TCC + G +GCCP A CC D+ CCP C + C+ E S P +K+ +L + V C D C + CC++ +GCC VCC D + CCP G C C + SI
Sbjct: 133 ASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDH-IPW---MSKLPARVK-----------SEGDEERRVP-----CLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTG----------EGPLLPQMPAIREESANQVPCDATTKK----ETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDG-------QTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQKGEHSIPLFSKTPAL----RQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQCMIGEHSI 501 HSP 3 Score: 119.013 bits (297), Expect = 1.145e-27 Identity = 87/289 (30.10%), Postives = 124/289 (42.91%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
G CCP C D ++CC + C C+ +S ++ CP+ +CP CC MS +GCC + +CC D CCPHN++CD + C+S ++++P + E + C D C TCC D YGCC +A CCSD+ CCP CDL K C ++ P + + ++ E +V C TK E CC++ ++GCC VCC D CCP G C G CSK++ SI
Sbjct: 97 GGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPAS-------------------------NQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTF-CPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAI---REESANQVPCDATTKKET---CCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSI 353 HSP 4 Score: 115.931 bits (289), Expect = 1.192e-26 Identity = 100/385 (25.97%), Postives = 159/385 (41.30%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS--DLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKN 1329
+ E++ N P + T KET CC + CC A+CC D HCCP G TC ++ C++ +E + W L + D++ ++ R+ G++ CCP AVCC D +CCP C +C G S L++ L + KC ++ C + CC ++ G +GCC + +CC D + CCP C S + + + S ++ P +Q+ ++ +++ C +TCC + G +GCCP A CCSD+ CCP CD + C + S P +TK +L G IC D C + + CC + + CC +
Sbjct: 290 IREESANQVPCDATTKKET--CCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSK-MEHSIPWFTKTPALTHEARDVE-CDDMYSCPDGQTCCRLAS----------------------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC-----------QKGEHSIPLFSKTPALRQETKYVKCDDT-HSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC-SGSQCMIGEHSIPWFSKTPALKQKAKDVQCDDM-----YSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCD-ARGTCVLGQFSIPWLTKVPALPF----DGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEE 621 HSP 5 Score: 106.301 bits (264), Expect = 1.495e-23 Identity = 67/198 (33.84%), Postives = 91/198 (45.96%), Query Frame = 1
Query: 790 CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGD-GLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP + C G CC +G C D CC + C C IP Q +CPD EC + TCC + D +GCCP A CC D+ CCPHN CD+++ C + + P ++K + + + +V C D T C + S CC+ D+ YGCCS + VCC+D CCP GT C
Sbjct: 85 CPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC---------IPASNQSAV------VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTC 267 HSP 6 Score: 100.138 bits (248), Expect = 1.596e-21 Identity = 114/413 (27.60%), Postives = 163/413 (39.47%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS--DLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKN------------ATCCSDNA---SCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCS 1398
++ C + QTCC + CC A+CC D HCCP G TC + C + E ++P+ S + LR ++ C D +TCC G++ CCP AVCC D +CCP C +C+ G S ++ L + +C + C + CC ++ G +GCC + +CC D CCP CD+ + + S LT++P ++IC D + C S TCC G + CCP + AT C S CP + +K + ETS PS T + L Y V C Q C + CC+ + CC Y GVCC D CCP G C++ G S
Sbjct: 372 DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQKG----------EHSIPLF--------------SKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCM-----------IGEHSIPWFSKTPALKQKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFD----GTHSICDDTHT-CPSGTTCCPGEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQK--EAIETS-PSDTSVQGLRLDY------VWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTS 730 HSP 7 Score: 63.5438 bits (153), Expect = 5.785e-10 Identity = 65/279 (23.30%), Postives = 103/279 (36.92%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCP-ENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL-------------LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS 942
I F+K + +K ++V ++ C + QTCC + CC A+CC D +HCCP G TCDA V + L P+ G S + DD + CCP + + CCP E +K L ++A + + ++ +Y+L + +L+ +S C + CC GG + CC + +G+CCPDM CCP+ C + +C S
Sbjct: 501 IPWFSKTPA--LKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGT-------------CVLGQFSIPWLTKVPALPFDGTHS------ICDDTHTCPS----------------GTTCCPGEGG-----GWRCCPVEEVSKMVKRTVILLATACIVTLVSFSF-CPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGS 736 HSP 8 Score: 51.2174 bits (121), Expect = 3.972e-6 Identity = 29/92 (31.52%), Postives = 42/92 (45.65%), Query Frame = 1
Query: 118 IIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEW 393
III YAL + + + + V +++ C + QTCC ++CC Y +CCPD HCCP G C C+R+ G W
Sbjct: 650 IIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRS--GSPRW 739
BLAST of Granulin vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 146.747 bits (369), Expect = 6.353e-37 Identity = 120/431 (27.84%), Postives = 176/431 (40.84%), Query Frame = 1
Query: 214 NRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC-TRNVEGPM--EWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL-TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGGCSKVK*SIKCLKLILYSTFEFTK-----FHECP 1476
+ T C + TCC ++Y CC +A+CC D HCCP G+TCD K+C ++ EGP+ + P + E + + P E+ + G + CCP AVCC+D +CCPE C ++ S + +T L + + +C + CP + CC ++ G +GCC + +CC D + CCP C + S L ++ P RQ +K D C +TCC + G +GCCP A CC D+ CCP C + C E S P +K+ +L + V C D C + CC++ +GCC VCC+D CCP G C G C + SI L + F+ T H CP
Sbjct: 205 DGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIRE----ESANQVPCDATTKKETCCRLVS-------------------------GKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPW-------------FTKTPALTHEARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETKYVKC-----DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ--CMIGEHSIPWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCP 581 HSP 2 Score: 146.362 bits (368), Expect = 9.872e-37 Identity = 124/416 (29.81%), Postives = 179/416 (43.03%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCV-TKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKS-SDLTEIPNERQREAQIKLNNI--CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
S ++ VCP+ R++C TCC+ + +S+ CC P A+CC D HCCP S CD + RC N + + W LP ++ E RV + T C D TCC Y CC +AVCCSD +CCP T CDL +KKC+ + + L ++ + C + K+ + CC + G +GCC K +CC D CCP C + + + S T+ P ++ +++ CPD +TCC + G +GCCP A CC D+ CCP C + C+ E S P +K+ +L V C D C + CC++ +GCC VCC D + CCP G C C + SI
Sbjct: 113 ASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDH-IPW---MSKLPARVK-----------SEGDEERRVP-----CLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTG----------EGPLLPQMPAIREESANQVPCDATTKK----ETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDG-------QTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQKGEHSIPLFSKTPALRQETK----YVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQCMIGEHSI 481 HSP 3 Score: 118.242 bits (295), Expect = 1.698e-27 Identity = 87/289 (30.10%), Postives = 124/289 (42.91%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SI 1416
G CCP C D ++CC + C C+ +S ++ CP+ +CP CC MS +GCC + +CC D CCPHN++CD + C+S ++++P + E + C D C TCC D YGCC +A CCSD+ CCP CDL K C ++ P + + ++ E +V C TK E CC++ ++GCC VCC D CCP G C G CSK++ SI
Sbjct: 77 GGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPAS-------------------------NQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTF-CPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAI---REESANQVPCDATTKKET---CCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSI 333 HSP 4 Score: 115.546 bits (288), Expect = 1.338e-26 Identity = 100/385 (25.97%), Postives = 159/385 (41.30%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS--DLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKN 1329
+ E++ N P + T KET CC + CC A+CC D HCCP G TC ++ C++ +E + W L + D++ ++ R+ G++ CCP AVCC D +CCP C +C G S L++ L + KC ++ C + CC ++ G +GCC + +CC D + CCP C S + + + S ++ P +Q+ ++ +++ C +TCC + G +GCCP A CCSD+ CCP CD + C + S P +TK +L G IC D C + + CC + + CC +
Sbjct: 270 IREESANQVPCDATTKKET--CCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSK-MEHSIPWFTKTPALTHEARDVE-CDDMYSCPDGQTCCRLAS----------------------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC-----------QKGEHSIPLFSKTPALRQETKYVKCDDT-HSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC-SGSQCMIGEHSIPWFSKTPALKQKAKDVQCDDM-----YSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCD-ARGTCVLGQFSIPWLTKVPALPF----DGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEE 601 HSP 5 Score: 105.916 bits (263), Expect = 2.029e-23 Identity = 67/198 (33.84%), Postives = 91/198 (45.96%), Query Frame = 1
Query: 790 CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGD-GLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP + C G CC +G C D CC + C C IP Q +CPD EC + TCC + D +GCCP A CC D+ CCPHN CD+++ C + + P ++K + + + +V C D T C + S CC+ D+ YGCCS + VCC+D CCP GT C
Sbjct: 65 CPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC---------IPASNQSAV------VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTC 247 HSP 6 Score: 99.7525 bits (247), Expect = 2.129e-21 Identity = 116/414 (28.02%), Postives = 163/414 (39.37%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKN------------ATCCSDNA---SCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCS 1398
++ C + QTCC + CC A+CC D HCCP G TC + C + E ++P+ S + LR ++ C D +TCC G++ CCP AVCC D +CCP C +C+ G S ++ L + +C + C + CC ++ G +GCC + +CC D CCP CD A+ CV LT++P ++IC D + C S TCC G + CCP + AT C S CP + +K + ETS PS T + L Y V C Q C + CC+ + CC Y GVCC D CCP G C++ G S
Sbjct: 352 DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQKG----------EHSIPLF--------------SKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCM-----------IGEHSIPWFSKTPALKQKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCD-ARGTCVLGQFSIPWLTKVPALPFD----GTHSICDDTHT-CPSGTTCCPGEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQK--EAIETS-PSDTSVQGLRLDY------VWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTS 710 HSP 7 Score: 63.1586 bits (152), Expect = 6.395e-10 Identity = 65/279 (23.30%), Postives = 103/279 (36.92%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCP-ENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL-------------LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS 942
I F+K + +K ++V ++ C + QTCC + CC A+CC D +HCCP G TCDA V + L P+ G S + DD + CCP + + CCP E +K L ++A + + ++ +Y+L + +L+ +S C + CC GG + CC + +G+CCPDM CCP+ C + +C S
Sbjct: 481 IPWFSKTPA--LKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGT-------------CVLGQFSIPWLTKVPALPFDGTHS------ICDDTHTCPS----------------GTTCCPGEGG-----GWRCCPVEEVSKMVKRTVILLATACIVTLVSFSF-CPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGS 716 HSP 8 Score: 51.2174 bits (121), Expect = 4.254e-6 Identity = 29/92 (31.52%), Postives = 42/92 (45.65%), Query Frame = 1
Query: 118 IIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEW 393
III YAL + + + + V +++ C + QTCC ++CC Y +CCPD HCCP G C C+R+ G W
Sbjct: 630 IIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRS--GSPRW 719
BLAST of Granulin vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 172.555 bits (436), Expect = 1.645e-46 Identity = 117/399 (29.32%), Postives = 167/399 (41.85%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSK---SSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
C +C +T + CC + + C D YHCCP G C A K C + + P+ V+ P S +S + + V+ G++ CCP A CC D+ +CCP CDL + +C+ + K + N V CP++ QCP CC + G +GCC ICC D CCP +T CD + C+SK + LT++P +E + D V C TCC + G +GCCPF A CC D+ CCP C EK C+ P + K + L LK C D T+C ++ CC+++ +GCC VCC+D Q CCP G C++ G
Sbjct: 75 QTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLG--AVQ----------CPGSQFECPDSATCCIMVD-----------------------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVV------CPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYPVKEVKC-------DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQG 425 HSP 2 Score: 169.859 bits (429), Expect = 1.454e-45 Identity = 121/403 (30.02%), Postives = 172/403 (42.68%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNV-----------ECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP ++ +C ++ TCC+ S+ CC P A CC D+ HCCP G++CD RC P + + P + + S +++T +D C G Y CCP NA+CCSD +CCP++T CDL KCL K+ L E KC + + CP CC ++ G +GCC F K +CC D CCP C + K C + ++P ++ A ++L PD + C + TCC + G +GCCP A CCSDN CCP C L + C+ +T + K + +IG + C T C CC + CC + VCC D+Q CCP G C
Sbjct: 138 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVS----PTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPT--------------GKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLS-------------KNYTTDLLTKLPGYPVKEVKC-DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTC---EMGILQVPWMKKVIAPLRL----PDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIG-DIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTC 499 HSP 3 Score: 150.984 bits (380), Expect = 7.437e-39 Identity = 119/432 (27.55%), Postives = 177/432 (40.97%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSET--------LRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSD-KQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACV---SKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLIL 1437
VS VCP+ +T+C + TCC Y CC PNAICC D HCCP + CD + +C + +N +L P + + + E R+N G + CCP AVCC D +CCP +C + C K + L + L+ P D +CP CC ++ G +GCC + +CC D + CCP C A+ C + + L +IP + QI +I D++ C +TCC G + CC +A CC D CCP C+++ + C K+ + QP + LL ++G V C + C ++ CC+ + CC Y GVCC D + CCP G C + G ++ I + L
Sbjct: 212 VSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKC------------LSKNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNT----------------------GAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKK-------VIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQI--GDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPV-----LLTLGPKVG-NVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFL 593 HSP 4 Score: 113.235 bits (282), Expect = 4.186e-26 Identity = 71/223 (31.84%), Postives = 99/223 (44.39%), Query Frame = 1
Query: 721 LYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
L + + L+ C + CP C G CC F+KG+ C D CCP C + K+C S + L + CP EC TCC + DG +GCCP A+CC D CCPH CDL C + + + K + + + V+CPD +T+C +DS CC++ +YGCC N +CC+D CCP T C
Sbjct: 58 LLDTWPRITSHHLDGSC-QTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDN--------------PLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVC 265
BLAST of Granulin vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 84.3445 bits (207), Expect = 5.984e-18 Identity = 61/218 (27.98%), Postives = 95/218 (43.58%), Query Frame = 1
Query: 787 QCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLIL 1437
+CP CC ++ G +GCC +G C D + + L +IP + QI +I D++ C +TCC G + CC +A CC D CCP C+++ + C K+ + QP + LL ++G V C + C ++ CC+ + CC Y GVCC D + CCP G C + G ++ I + L
Sbjct: 47 RCPTNNTCCKLNSGDWGCCPIPEGYC---------QKGD---------TMVAGLEKIPARQTTPLQI--GDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPV-----LLTLGPKVG-NVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFL 238 HSP 2 Score: 67.3958 bits (163), Expect = 4.427e-12 Identity = 48/152 (31.58%), Postives = 71/152 (46.71%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCL 666
+ T C QTCC + S+ CC+ P+A+CC D+ HCCP+G TC+ + C ++V+ ++ P +L L P G E +D+ +TCC G + CCP+ VCC D +CCP C + KCL
Sbjct: 101 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVD------FIQ---PPVLLTLGPKV---GNVECGEGHFCHDN-------------QTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCL 227
BLAST of Granulin vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 77.7962 bits (190), Expect = 6.607e-16 Identity = 54/185 (29.19%), Postives = 76/185 (41.08%), Query Frame = 1
Query: 721 LYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKC 1266
L + + L+ C + CP C G CC F+KG+ C D CCP C + K+C S + L + CP EC TCC + DG +GCCP A+CC D CCPH CDL C + + + K + + + V+CPD +T+C
Sbjct: 45 LLDTWPRITSHHLDGSC-QTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDN--------------PLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQC 214 HSP 2 Score: 75.485 bits (184), Expect = 4.659e-15 Identity = 45/159 (28.30%), Postives = 62/159 (38.99%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQ-REAQIKLNNICPDKNVEC 1026
G CCP V C D Y+CCP+ C K C + + + +CP S +CP CC M G +GCC + CC D CCPH CD CVS + L + P ++ R + + +CPD +C
Sbjct: 79 GTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC-----------------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQC 214 HSP 3 Score: 56.225 bits (134), Expect = 1.383e-8 Identity = 23/54 (42.59%), Postives = 33/54 (61.11%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCT 366
CP ++ +C ++ TCC+ S+ CC P A CC D+ HCCP G++CD RC
Sbjct: 125 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCV 178 HSP 4 Score: 55.8398 bits (133), Expect = 2.251e-8 Identity = 45/194 (23.20%), Postives = 69/194 (35.57%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQC 792
C +C +T + CC + + C D YHCCP G C A K C + + P+ V+ P S +S + + V+ G++ CCP A CC D+ +CCP CDL + +C+ + K + N V CP++ QC
Sbjct: 62 QTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLG--AVQ----------CPGSQFECPDSATCCIMVD-----------------------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVV------CPDAKTQC 214 HSP 5 Score: 53.5286 bits (127), Expect = 1.189e-7 Identity = 36/121 (29.75%), Postives = 47/121 (38.84%), Query Frame = 1
Query: 1021 ECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
C + +C G CCPF C D CCP C + K C + +G V CP Q +C + + CC M D +GCC CC D+ CCP+G C
Sbjct: 66 HCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDN---------------PLG-AVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASC 170
BLAST of Granulin vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 79.7221 bits (195), Expect = 9.234e-16 Identity = 54/183 (29.51%), Postives = 86/183 (46.99%), Query Frame = 1
Query: 787 QCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACV---SKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSY 1323
+CP CC ++ G +GCC + +CC D + CCP C A+ C + + L +IP + QI +I D++ C +TCC G + CC +A CC D CCP C+++ + C K+ + QP + LL ++G V C + C ++ CC+ + CC Y
Sbjct: 4 RCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQI--GDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPV-----LLTLGPKVG-NVECGEGHFCHDNQTCCKDSAGVWACCPY 177 HSP 2 Score: 74.3294 bits (181), Expect = 5.432e-14 Identity = 42/121 (34.71%), Postives = 55/121 (45.45%), Query Frame = 1
Query: 1018 VECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
C + TCC + G +GCCP A CCSDN CCP C L + C+ +T + K + +IG + C T C CC + CC + VCC D+Q CCP G C
Sbjct: 3 TRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIG-DIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTC 121 HSP 3 Score: 65.4698 bits (158), Expect = 4.274e-11 Identity = 51/174 (29.31%), Postives = 73/174 (41.95%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPF 1083
G++ CCP AVCCSD +CCP+ C L C + AGL+ L D C + CP + CCP G + CC+ +CC D + CCP C+ + C K D + P K+ N+ + C +TCC G++ CCP+
Sbjct: 17 GDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMV-----AGLEKIPARQTTPLQIGD---IGC-DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC-EKDVDFIQPPVLLTLGP--KVGNVECGEGHFCHDNQTCCKDSAGVWACCPY 177 HSP 4 Score: 55.8398 bits (133), Expect = 6.163e-8 Identity = 23/56 (41.07%), Postives = 36/56 (64.29%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVE 378
+ T C QTCC + S+ CC+ P+A+CC D+ HCCP+G TC+ + C ++V+
Sbjct: 78 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVD 133 HSP 5 Score: 51.6026 bits (122), Expect = 1.231e-6 Identity = 45/151 (29.80%), Postives = 69/151 (45.70%), Query Frame = 1
Query: 220 TKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRN---VEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC 663
T+C TCC + CC P A+CC D HCCP G TC A+ C + V G +E + P+++ D+ G + ++ C +TCC + G++ CC +AVCC D+ +CCP C++K + C
Sbjct: 3 TRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAG-LEKIPARQTTPLQIGDI-------GCDQHTS----------------CPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC 128
BLAST of Granulin vs. UniProt/SwissProt
Match: sp|P28798|GRN_MOUSE (Progranulin OS=Mus musculus OX=10090 GN=Grn PE=1 SV=2) HSP 1 Score: 172.17 bits (435), Expect = 1.341e-45 Identity = 117/399 (29.32%), Postives = 167/399 (41.85%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSK---SSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
C +C +T + CC + + C D YHCCP G C A K C + + P+ V+ P S +S + + V+ G++ CCP A CC D+ +CCP CDL + +C+ + K + N V CP++ QCP CC + G +GCC ICC D CCP +T CD + C+SK + LT++P +E + D V C TCC + G +GCCPF A CC D+ CCP C EK C+ P + K + L LK C D T+C ++ CC+++ +GCC VCC+D Q CCP G C++ G
Sbjct: 62 QTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLG--AVQ----------CPGSQFECPDSATCCIMVD-----------------------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVV------CPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYPVKEVKC-------DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQG 412 HSP 2 Score: 169.474 bits (428), Expect = 1.167e-44 Identity = 121/403 (30.02%), Postives = 172/403 (42.68%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNV-----------ECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP ++ +C ++ TCC+ S+ CC P A CC D+ HCCP G++CD RC P + + P + + S +++T +D C G Y CCP NA+CCSD +CCP++T CDL KCL K+ L E KC + + CP CC ++ G +GCC F K +CC D CCP C + K C + ++P ++ A ++L PD + C + TCC + G +GCCP A CCSDN CCP C L + C+ +T + K + +IG + C T C CC + CC + VCC D+Q CCP G C
Sbjct: 125 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVS----PTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPT--------------GKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLS-------------KNYTTDLLTKLPGYPVKEVKC-DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTC---EMGILQVPWMKKVIAPLRL----PDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIG-DIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTC 486 HSP 3 Score: 150.214 bits (378), Expect = 6.670e-38 Identity = 119/432 (27.55%), Postives = 177/432 (40.97%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSET--------LRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSD-KQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACV---SKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLIL 1437
VS VCP+ +T+C + TCC Y CC PNAICC D HCCP + CD + +C + +N +L P + + + E R+N G + CCP AVCC D +CCP +C + C K + L + L+ P D +CP CC ++ G +GCC + +CC D + CCP C A+ C + + L +IP + QI +I D++ C +TCC G + CC +A CC D CCP C+++ + C K+ + QP + LL ++G V C + C ++ CC+ + CC Y GVCC D + CCP G C + G ++ I + L
Sbjct: 199 VSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKC------------LSKNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNT----------------------GAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKK-------VIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQI--GDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPV-----LLTLGPKVG-NVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFL 580 HSP 4 Score: 112.849 bits (281), Expect = 3.557e-25 Identity = 71/223 (31.84%), Postives = 99/223 (44.39%), Query Frame = 1
Query: 721 LYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
L + + L+ C + CP C G CC F+KG+ C D CCP C + K+C S + L + CP EC TCC + DG +GCCP A+CC D CCPH CDL C + + + K + + + V+CPD +T+C +DS CC++ +YGCC N +CC+D CCP T C
Sbjct: 45 LLDTWPRITSHHLDGSC-QTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDN--------------PLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVC 252
BLAST of Granulin vs. UniProt/SwissProt
Match: sp|P28799|GRN_HUMAN (Progranulin OS=Homo sapiens OX=9606 GN=GRN PE=1 SV=2) HSP 1 Score: 170.244 bits (430), Expect = 6.707e-45 Identity = 130/425 (30.59%), Postives = 189/425 (44.47%), Query Frame = 1
Query: 139 TDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---------VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKE--TSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
DG F ++ + V CP+++ +C + TCCV S+ CC P A CC D+ HCCP G+ CD RC P + + LP + + + ++ + CP+ +++C D TCC G Y CCP NA CCSD +CCP++T CDL KCL S +NA + +L T V + KC + + CP CC + G +GCC F + +CC D CCP CD+ K C + K+ +P+ + + + +N+ C S +TCC + G +GCCP A CCSD+ CCP C E + + E + ++ L +IG C T C CC + CC + VCC D+Q CCP G C
Sbjct: 107 ADGRSCFQRSGNNSVGAIQ---CPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT----PTGTHPLAKKLPA--------------QRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL-------SKENA---TTDLLTKLPAHTVGDV--KC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIG----CDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTC 488 HSP 2 Score: 167.162 bits (422), Expect = 7.508e-44 Identity = 115/407 (28.26%), Postives = 185/407 (45.45%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK-LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK-VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
V+ + +CP+ R++C + TCC Y CC PNA CC D HCCP + CD + +C + +EN +L P+ T+ + + E + D TC ++ G + CCP AVCC D +CCP CD + C ++ ++ A + SL + L++ P ++ CP CC ++ G +GCC + +CC D + CCP C A+ C S + + R A + +I D++ C +TCC G + CC +A CC D CCP C+++ + C+ + S + + L +G+K V C + C ++ CC+ + + CC Y+ GVCCAD++ CCP G +C + G
Sbjct: 200 VALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKC-----------LSKENATTDLLTKLPAHTVGDVKCDME-VSCPDGYTCCRLQS-------------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQ-----GPHQVPWMEKAPAH--LSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVS----AQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARG 569 HSP 3 Score: 145.591 bits (366), Expect = 3.775e-36 Identity = 88/277 (31.77%), Postives = 122/277 (44.04%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQ-REAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
G CCP AV C D ++CCP C + C ++ +G S +CP+S +CP CC M G +GCC + CC D CCPH CD C++ + ++P +R R + + +CPD C TCC + G YGCCP NATCCSD+ CCP + VCDL + C +KE + + L + V C + C + CC++ +GCC + VCC D CCP G C
Sbjct: 79 GTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSC---------FQRSGNNSVGAI-------------QCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDL-----LTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTC 328 HSP 4 Score: 114.005 bits (284), Expect = 1.476e-25 Identity = 82/324 (25.31%), Postives = 133/324 (41.05%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQ 1182
+ C + TCC + ++ CC + A+CC D HCCP+G TCD K C +GP + P +E+ P + P + ++ + + D C G + CCP AVCCSD +CCP+ C + +C S + + + A+L + + D+ CP + CCP GG + CC+ +CC D + CCP C+ ++C K + R + + ++ + C +TCC + CCP++ CC+D CCP C C +E +
Sbjct: 285 DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCE---QGPHQVPWMEK-APAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTS--------------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASL-SHPRDIGCDQ--------HTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC-EKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPR 579 HSP 5 Score: 112.079 bits (279), Expect = 6.254e-25 Identity = 68/204 (33.33%), Postives = 89/204 (43.63%), Query Frame = 1
Query: 781 DKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL--KVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
D C C G CC F + + C D CCP C + ++C QR + I CPD EC F TCC + DG +GCCP A+CC D CCPH CDL C + P + K + L V+CPD +++C + S CC++ +YGCC N CC+D CCP T C
Sbjct: 64 DAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCF-------------QRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHP-LAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVC 253 HSP 6 Score: 75.0998 bits (183), Expect = 5.361e-13 Identity = 66/253 (26.09%), Postives = 106/253 (41.90%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVE--ENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSL-LNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP 960
+N + C + TCC + CC P A+CC D HCCP G TC A+ +C R E +V E +P + L + + S + +TCC + G++ CC +AVCC D+ +CCP C++K + C + SA TF + +V + +C C + CC + + CC + +G+CC D + CCP C + C+ + + + P
Sbjct: 367 DNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSE------IVAGLEKMPARRASLSHPRDIGCDQHTSCPV-----------------GQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC-----------EKEVVSAQPATFLARSPHVGVKDVEC-GEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAP 583
BLAST of Granulin vs. UniProt/SwissProt
Match: sp|P23785|GRN_RAT (Progranulin OS=Rattus norvegicus OX=10116 GN=Grn PE=1 SV=3) HSP 1 Score: 167.933 bits (424), Expect = 4.411e-44 Identity = 118/400 (29.50%), Postives = 169/400 (42.25%), Query Frame = 1
Query: 217 RTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTR---NVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSK--SSDL-TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK--VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
R C + +C +T + CC + + C D HCCP G C A K C++ ++ G ++ P + P +S T + D G++ CCP A CC D+ +CCP CDL + +C+ + K + N V CP++ QCP CC + G +GCC ICC D CCP +T CD + C+SK ++DL T++P E + L CPD TCC + G +GCCPF A CC D+ CCP C E C+ P + K + L LK V C D + C +++ CC++ +GCC VCC D Q CCP G +C+ G
Sbjct: 64 RDHCPDGYSCLLTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSCSQISDSLLGAVQCPGSQFECP-----------------DSATCCIMID---------------------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTHPLLKKFPAQRTNR-------AVASFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTDLMTKLPGYPVNEVKCDLEVSCPDGY-------TCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKCMDEG 411 HSP 2 Score: 158.688 bits (400), Expect = 8.654e-41 Identity = 119/405 (29.38%), Postives = 175/405 (43.21%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFE---SNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKL-------NNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITK---SKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP ++ +C ++ TCC+ S+ CC P A CC D+ HCCP G++CD RC P L K + + + F +++T +D C G Y CCP NA+CCSD +CCP++T CDL KC+ + +L T V+ E KC + + CP CC ++ G +GCC F K +CC D CCP C + C + ++P ++ A + L N++ D C S TCC + G +GCCP A CC D+ CCP C +++ C+ + + K ++ L + +IG C T C CC + CC + VCC D+Q CCP G C
Sbjct: 125 CPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCIS--------PTGTHPLLKKFPAQRTNRAVASFSVVCPDAKTQCPDDSTCCELPT--------------GKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKD-----------YTTDLMTKLPGYPVN--EVKC-DLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTC---ELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPVRQTTLLQHGDIG----CDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTC 485 HSP 3 Score: 147.132 bits (370), Expect = 7.903e-37 Identity = 115/423 (27.19%), Postives = 174/423 (41.13%), Query Frame = 1
Query: 157 FNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSET--------LRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSD-KQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQI-KLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
F ++ VCP+ +T+C + TCC Y CC PNAICC D HCCP + CD + +C + ++ ++ P ++ + + E R+N G + CCP AVCC D +CCP +C + C K +A+L SL + L+ P D CP CC +S G +GCC + +CC D + CCP C + C + + R+ + + +I D++ C +TCC G + CC +A CC D CCP C+++ + C K+ + QPS+ L F S++G V C C ++ CC+ + CC Y GVCC D + CCP G C + G
Sbjct: 190 FPAQRTNRAVASFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKC------------ISKDYTTDLMTKLPGYPVNEVKCDLEVSCPDGYTCCRLNT----------------------GAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKV---TASL----SLPDPQILKNDVPCDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSVQPSMD-----LTFGSKVG-NVECGAGHFCHDNQSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKG 564 HSP 4 Score: 121.324 bits (303), Expect = 6.653e-28 Identity = 72/221 (32.58%), Postives = 100/221 (45.25%), Query Frame = 1
Query: 721 LYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
L + ++ +L+ C D CP C G CC F++G+ C D + CCP C + K+C S L CP EC TCC + DG +GCCP A+CC D CCPH CDL C + + P + K + + V+CPD +T+C +DS CC++ +YGCC N +CC+D CCP T C
Sbjct: 45 LLDTWPIITSRRLDGSCQIRD-HCPDGYSCLLTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSCSQISDSLLGAVQ-------------CPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTHPLLKKFPAQRTNRAVASFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVC 251 HSP 5 Score: 98.9821 bits (245), Expect = 1.333e-20 Identity = 79/329 (24.01%), Postives = 120/329 (36.47%), Query Frame = 1
Query: 217 RTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENL----PVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSI 1191
C + TCC ++ CC + A+CC D HCCP+G C C V V +L P + + P SN+ R++ G++ CCP AVCC D +CCP+ KC + C + + ++ L + + D+ CP + CCP G + CC+ +CC D + CCP C+ + C D + K+ N+ C ++CC G + CCP+ CC D CCP C + C K+T + I
Sbjct: 284 EVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFSSCPSNNTCCRLSS----------------------GDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPVRQTTLLQ-HGDIGCDQ--------HTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC---EKDAGSVQPSMDLTFGSKVGNVECGAGHFCHDNQSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKGTKCLRKKTPRWDI 577
BLAST of Granulin vs. UniProt/SwissProt
Match: sp|P28797|GRN_CAVPO (Progranulin OS=Cavia porcellus OX=10141 GN=GRN PE=1 SV=2) HSP 1 Score: 143.28 bits (360), Expect = 2.480e-35 Identity = 87/291 (29.90%), Postives = 127/291 (43.64%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQR---------------EAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
G CCP A+ C D ++CCP C C ++ +++ ++ +CP + +CP CC M G +GCC + CC D CCPH CD CV+ T++P +R A + + ICPD +C TCC + G YGCCP NA CCSD+ CCP + VCDL + C ++ ++ +TK S + E +V CP+ CC++ ++GCC + VCC D CCP G +C
Sbjct: 79 GTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTC--------------IQRPDIHLLGAV--------QCPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTKLPSWTVWDVECDQEVSCPE------GQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRC 341 HSP 2 Score: 138.272 bits (347), Expect = 1.404e-33 Identity = 116/421 (27.55%), Postives = 162/421 (38.48%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEG-PM--EWPVVE------ENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKL--------------------NNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP +C ++ TCC S+ CC P A CC D+ HCCP G++CD RC + P+ + P E PV L P++ + DD TC + G Y CCP NA+CCSD +CCP++T CDL+ +CL + K+ L T V +E + + CP + CC + G +GCC F K +CC D CCP C + K C L ++P ++ AQ + I D+ V C CC + G +GCCP C + + C+ + P K + L S+ V C C CC + CC + VCC D Q CCP G C
Sbjct: 126 CPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLAS-------------GEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQN-----------KAKTLLTKLPSWTVWDVEC---DQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTC---EQGLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPLRSEISCDEVVSCAPGNICCRLASGEWGCCPSSEGYLC--------------MAGERCQVGDRLAPE--KMAAHLMSLSQT-TDVGCDQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTC 499 HSP 3 Score: 134.42 bits (337), Expect = 2.646e-32 Identity = 101/408 (24.75%), Postives = 158/408 (38.73%), Query Frame = 1
Query: 226 CKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTR----NVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC--------LDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKS---SDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKS---------------LLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTC 1359
C +C +T + CC + A+ C D +HCCP G C C + ++ G ++ P E P S+ H + G++ CCP A CC D+ +CCP CDL + +C L + G + + + L CP+S QCP CC ++ G +GCC ICC D CCP +T CD + C+S++ + LT++P+ + + D+ V C +TCC + G +GCCPF A CC D+ CCP C EK C+ P K+ + + ++ C + C ++CC++ ++GCC G C + C
Sbjct: 67 CPIGHSCVLTAAGTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTCIQRPDIHLLGAVQCPGGEFECP------DSSTCCHMLD--------------------------------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTKLPSWTVWDVEC-------DQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQGLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPLRSEISCDEVVSCAPGNICCRLASGEWGCCPSSEGYLCMAGERC 429 HSP 4 Score: 128.257 bits (321), Expect = 3.436e-30 Identity = 105/406 (25.86%), Postives = 160/406 (39.41%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVD--------KLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIG-LKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
+CP++R++C + TCC+ Y CC PNAICC D HCCP + CD + RC + +N +L PS T+ E + E C + +TCC + G + CCP AVCC D +CCPE +C + C + + + + E C PG CC ++ G +GCC ++G C + C L + + ++ D++ C +TCC G + CC +A CC D CCP C+++ + C+ + L S G + V C D+ C ++ CC+ + CC + GVCC D++ CCP G C S G
Sbjct: 221 ICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRC------------LSQNKAKTLLTKLPSWTVWDVECDQEV--------------SCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQGLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPLRSEISCDEVVSCAPG-NICCRLASGEWGCCPSSEGYLCMAGERC---------------QVGDRLAPEKMAAHLMSLSQTTDVGCDQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEKAADG----AHLAAPLAVGSTGGVMDVACGDRHFCHDEQTCCRDSRGGWACCPFHQGVCCKDQRHCCPAGFHCESQG 580 HSP 5 Score: 109.768 bits (273), Expect = 3.926e-24 Identity = 70/219 (31.96%), Postives = 93/219 (42.47%), Query Frame = 1
Query: 775 NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFY---------------SEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
S CP C + G CC F++ + C D CCP+ C + C+ QR L + CP EC TCC + DG +GCCP A+CC D CCPH CDL C S P TK + Y + + VICPD +++C +D+ CC + +YGCC N +CC+D CCP T C
Sbjct: 62 QSPANCPIGHSCVLTAAGTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTCI-------------QRPDIHLLGAVQCPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVC 267 HSP 6 Score: 94.3597 bits (233), Expect = 4.307e-19 Identity = 84/345 (24.35%), Postives = 132/345 (38.26%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKK--CLDSSAVFNSYKN----AGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYS 1224
+ C E QTCC + + CC +P A+CC D HCCP G C K C +G ++ P ++ P + S S + +I+ C + +C P N C L + + C SS + G + A L+++ + + CP + CCP GG + CC+ +CC D + CCP C+ ++C K++D + + + ++ C D++ C +TCC G + CCPF CC D CCP C+ + C +K KSLLH+ S
Sbjct: 298 DQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCE---QGLLQVPWAQKT-PAQPSRPSQPSPPGPPGPPSPPGPLRSEIS--------------------------------CDEVVSCAPGNICCRLASGEWGCCPSSEGYLCMAGERCQVGDRLAPEKMAAHLMSLSQTTDVGCDQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSC-EKAADGAHLAAPLAVGSTGGVMDVACGDRHF-CHDEQTCCRDSRGGWACCPFHQGVCCKDQRHCCPAGFHCESQGTRCVHK----------KSLLHWDS 594
BLAST of Granulin vs. UniProt/SwissProt
Match: sp|Q54QR7|GRN_DICDI (Granulin OS=Dictyostelium discoideum OX=44689 GN=grn PE=3 SV=1) HSP 1 Score: 51.9878 bits (123), Expect = 7.762e-7 Identity = 34/88 (38.64%), Postives = 45/88 (51.14%), Query Frame = 1
Query: 934 KSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITK 1197
KS +LTE Q +K CPD ++ C + TCCS DG Y CCP NA CCSD CCP+ C +CK ++ + S +
Sbjct: 52 KSLELTET----QDFGSVK----CPDGSL-CPNSNTCCSASDGSYACCPTPNAQCCSDKQHCCPYQFTCGNGGNICKPQQGLRFSFNR 130 HSP 2 Score: 49.6766 bits (117), Expect = 4.008e-6 Identity = 24/51 (47.06%), Postives = 28/51 (54.90%), Query Frame = 1
Query: 1240 VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
V CPD + C N + CC D Y CC N CC+DKQ CCP C +GG
Sbjct: 65 VKCPDGSLCPNSNTCCSASDGSYACCPTPNAQCCSDKQHCCPYQFTCGNGG 115
BLAST of Granulin vs. TrEMBL
Match: V4ARK5 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_146967 PE=4 SV=1) HSP 1 Score: 211.46 bits (537), Expect = 1.147e-59 Identity = 143/393 (36.39%), Postives = 197/393 (50.13%), Query Frame = 1
Query: 226 CKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC--LDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ-IKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSG 1389
C + TCC Y CC PNA+CC D+ HCCPSG+TCD +C R + W +++S++L+V +++ CP+ +++C D TCC G Y CCP NAVCCSD+ +CCP T CD KC D ++V S K T L V+ + CP+ QCP CC +S G +GCC +CC D CCP T CD+ C D T + ++ +Q +K+ N+ CPD+ +C TCC + G YGCCP NA CCSD CCP CD C ++ + +K S E V+CPD Q++C + S CC++ QYGCC N VCC+D+ CCP+GT C +G
Sbjct: 1 CPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMW---------------------SKKTSSQSLKV-ENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKK----------TSSQSLKVENVV--CPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC--NRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVE---NVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTG 354 HSP 2 Score: 177.948 bits (450), Expect = 5.806e-47 Identity = 122/360 (33.89%), Postives = 174/360 (48.33%), Query Frame = 1
Query: 157 FNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC--LDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ-IKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYS 1224
++K + Q + VCP+ +++C + TCC Y CC PNA+CC D+ HCCPSG+TCD +C R + W +++S++L+V +++ CP+ +++C D TCC G Y CCP NAVCCSD+ +CCP T CD KC D ++V S K T L V+ + CP+ QCP CC +S G +GCC +CC D CCP T CD+ C D T + ++ +Q +K+ N+ CPD+ +C TCC + G YGCCP NA CCSD CCP CD C ++ + +K S S
Sbjct: 56 WSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMW---------------------SKKTSSQSLKV-ENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKK----------TSSQSLKVENVV--CPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC--NRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCDRQDGTSVMWSKKTSSQSLKS 379 HSP 3 Score: 69.707 bits (169), Expect = 5.481e-9 Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 1
Query: 157 FNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDL 435
++K + Q + VCP+ +++C + TCC Y CC PNA+CC D+ HCCPSG+TCD +C R + W + +K D+
Sbjct: 290 WSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCDRQDGTSVMWSKKTSSQSLKSADV 382
BLAST of Granulin vs. TrEMBL
Match: A0A2B4SIQ6 (Granulin OS=Stylophora pistillata OX=50429 GN=GRN PE=4 SV=1) HSP 1 Score: 219.164 bits (557), Expect = 4.775e-58 Identity = 148/416 (35.58%), Postives = 202/416 (48.56%), Query Frame = 1
Query: 184 SEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKK-------CPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK----LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
S+ N VCP+ ++C + TCC Y CC P A+CC D HCCP G TCD + CT+ + M KP+ ESN I CP+ +++C D TCC G Y CCP AVCCSD +CCP CD+ +AG S + L + +++K CP+ + QCP CC +S G +GCC +CC D + CCP+ CD + +C SS IP ++ A ++ N +CPD EC TCC + G YGCCP+ NA CCSD CCP+ CD+ C +S P + K +L+ + G V+CPD Q++C + + CC++ QYGCC Y N VCC+D + CCPNG C VS G
Sbjct: 236 SDANSVVCPDGESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPDGYTCDVSAGTCTQGSSS------------IAMFQKKPAMKRESAESN---------IVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDV----------------SAGTCSKGTTSITFLQKLPAVQRKSDANSVVCPDGESQCPDGTTCCKLSTGQYGCCPLPNAVCCSDGEHCCPNGYTCDVSAGSCTQGSS---SIPMVQKLPALMREKSASNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSIPMVQKLPALMR--EKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAG 609 HSP 2 Score: 218.779 bits (556), Expect = 6.365e-58 Identity = 143/426 (33.57%), Postives = 206/426 (48.36%), Query Frame = 1
Query: 181 VSEKNEN--VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNN----ICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKL 1431
+ EKN + VCP+ +++C + TCC Y CC YPNA+CC D HCCP+G TCD + CT+ P++++ LP ++ + +S + SE N TCC G Y CCP+ NAVCCSD+ +CCP CD+ C S+ ++ Y + CP+ + +CP CC +S G +GCC +CC D CCP+ CD + C SS IP ++ A ++ N +CPD EC TCC + G YGCCP+ NA CCSD CCP+ CD+ C +S P + + +L + V+CP Q++C + + CC++ QYGCC N VCC+D + CCPNG C +G C + SI L++
Sbjct: 549 MREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQESS---SMPMMQK-LPA-LIRERNASNVVCPGGQSECPDGN----------------TCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAGTCTQGSSSIEMFQKMPAMKRESAASYVV---------CPDGESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCTQGSS---SIPMVQKLAALMREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSMPMVQRLPALKR--EKDASNVVCPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSSIASLQM 939 HSP 3 Score: 214.927 bits (546), Expect = 1.728e-56 Identity = 147/413 (35.59%), Postives = 200/413 (48.43%), Query Frame = 1
Query: 181 VSEKNEN--VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPN----ERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHF-YSEIGLKVICPDQT-KCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ E+N + VCP +++C + TCC Y CC YPNA+CC D+ HCCPSG TCD + CT+ ++M P+ ES + + D +++C D TCC G Y CCP NAVCCSD +CCP CD+ C S+ + K A L + NV CP+ +CP CC +S G +GCC + +CC D + CCP+ CD + C SS + + +R+++A N +CP EC TCC + G YGCCP NA CCSD CCP+ CD+ CK +S S+ +L Y + V+CPD+ +C N S CC++ YGCC KN VCC+D CCPNG C V GG
Sbjct: 628 IRERNASNVVCPGGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAGTCTQGSSS------------IEMFQKMPAMKR---ESAASYVVCPDG------ESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCTQGSSSIPMVQ----KLAALMREKNGSNV-----VCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSMPMVQRLPALKREKDAS---NVVCPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSSIASLQMHAALQAVTYIKAVDGVLCPDKKYQCPNGSTCCRVPSGTYGCCPIKNAVCCSDHIHCCPNGYTCDVEGG 1007 HSP 4 Score: 211.46 bits (537), Expect = 2.385e-55 Identity = 143/422 (33.89%), Postives = 205/422 (48.58%), Query Frame = 1
Query: 145 GIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNN----ICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+F A + + +E N VCP+ ++C + TCC Y CC P A+CC D HCCP+G TCD + C++ + L P+ +S++ ++ D +++C D TCC G Y CCP NAVCCSD +CCP CD+ C S+ + K L S NV CP+ +CP CC +S G +GCC + +CC D + CCP+ CD + C SS IP ++ A ++ N +CPD EC TCC + G YGCCP+ NA CCSD CCP+ CD+ C + +S P + K +L+ + V+CP Q++C + + CC++ QYGCC Y N VCC+D++ CCP+G C VS G
Sbjct: 303 AMFQKKPAMKRESAESNI-VCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSAGTCSKGTTS------------ITFLQKLPAVQR---KSDANSVVCPDG------ESQCPDGTTCCKLSTGQYGCCPLPNAVCCSDGEHCCPNGYTCDVSAGSCTQGSSSIPMVQ----KLPALMREKSASNV-----VCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSS---SIPMVQKLPALMREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQESSSMPMMQKLPALIRERN--ASNVVCPGGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAG 688 HSP 5 Score: 211.075 bits (536), Expect = 2.778e-55 Identity = 146/447 (32.66%), Postives = 215/447 (48.10%), Query Frame = 1
Query: 97 VKFMILT------IIIDYALT-----DGIFSFNK-ASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ FMIL II+ A T I F K ++ + S + VCP+ ++C + TCC Y CC P A+CC D HCCP+G TCD + C++ ++ + LP +S++ ++ D +++C D TCC G Y CCP AVCCSD +CCP+ CD+ C S+ ++ K + + N+ CP+ + +CP CC +S G +GCC K +CC D + CCP+ CD + C ++ +T + + + N++ CPD +C TCC + G YGCCP NA CCSD CCP+ CD+ C +S P + K +L+ S V+CPD Q++C + + CC++ QYGCC Y N VCC+D + CCPNG C VS G
Sbjct: 116 IIFMILILCLTCIIIVVNAGTCTQGSSSIAMFQKKPAKKRESAASNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDISAGTCSKGTT----TLILLQKLPAVQR-----------KSDANSVVCPDG------ESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPDGYTCDVSAGTCTQGSSSIAMFQ----KKPAMKRESAESNI-----VCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSAGTCSKGTTSITFLQKLPAVQRKSDANSVVCPDGESQCPDGTTCCKLSTGQYGCCPLPNAVCCSDGEHCCPNGYTCDVSAGSCTQGSSSIPMVQKLPALMREKS--ASNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAG 530 HSP 6 Score: 202.216 bits (513), Expect = 3.665e-52 Identity = 135/404 (33.42%), Postives = 182/404 (45.05%), Query Frame = 1
Query: 184 SEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLD-SSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI----CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
S + VCP+ ++C + TCC Y CC PNA+CC D HCCP+G TCD + CT +G P+V++ ++ K S + + SE N TCC G Y CCP+ NAVCCSD +CCP CD+ C SS++ + LK D CP +CP CC +S G +GCC +CC D K CCP+ CD + C SS + + +A + + CPDK +C + TCC V G YGCCP KNA CCSD+ CCP+ CD+E C I S + CPD C + CC + D ++GCC + VCC D + CCP+G +C
Sbjct: 710 SAASYVVCPDGESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCT---QGSSSIPMVQKL--AALMREKNGSNVVCPDGQSECPDGN----------------TCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSMPMVQRLPALKREK----------DASNVVCPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSSIASLQMHAALQAVTYIKAVDGVLCPDKKYQCPNGSTCCRVPSGTYGCCPIKNAVCCSDHIHCCPNGYTCDVEGGKCIKNAEHIKMIANVPSSRSSVDTEQKYIKCPDGCSCLDYETCCVLSDGRFGCCPLPHAVCCEDHEHCCPSGYRC 1082 HSP 7 Score: 168.703 bits (426), Expect = 8.950e-41 Identity = 136/490 (27.76%), Postives = 209/490 (42.65%), Query Frame = 1
Query: 103 FMILTIIIDYALTDGI---FSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVV--------------EENLPVKMLDL----------------KPSSTLHGFESNSETLR--VNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKK-------CPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNE-RQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLI 1434
F +L + + D + + + +++ + VCP +C +CC N D+ HCCP G +C++ + TR +P V + ++ + M+ + + SS++ F+ R +I CP+ +++C D TCC G Y CCP AVCCSD +CCP CD+ +AG S T L + +++K CP+ + QCP CC + G +GCC K +CC D K CCP CD + C SS + + + + N +CPD EC TCC + G YGCCP A CCSD CCP+ CD+ C TS + K ++ V+CPD +++C + + CC++ QYGCC N VCC+D + CCPNG C +G C++ SI ++ +
Sbjct: 9 FTLLCSQVTFCNADELKENTVLSAFTSNEIDVLDAVVCPGGVRQCPSGHSCCSVPGN--------------DEVHCCPVGYSCESGVCQETRKDNIAALFPSVGAKIVKCDNQYACPDRSIIIFMILILCLTCIIIVVNAGTCTQGSSSIAMFQKKPAKKRESAASNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDI----------------SAGTCSKGTTTLILLQKLPAVQRKSDANSVVCPDGESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPDGYTCDVSAGTCTQGSSSIAMFQKKPAMKRESAESNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSAGTCSKGTTSITFLQKLPAVQR--KSDANSVVCPDGESQCPDGTTCCKLSTGQYGCCPLPNAVCCSDGEHCCPNGYTCDVSAGSCTQGSSSIPMVQKL 466 HSP 8 Score: 167.162 bits (422), Expect = 2.948e-40 Identity = 117/347 (33.72%), Postives = 158/347 (45.53%), Query Frame = 1
Query: 181 VSEKNEN--VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI---CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKS 1206
+ EKN + VCP+ +++C + TCC Y CC YPNA+CC D HCCP+G TCD + CT +G P+V+ LP + K +S + SE N TCC G Y CCP NAVCCSD +CCP CD+ C S+ S + A L + VD + CP+ QCP CC + G +GCC +CC D CCP+ CD C+ + + I N + + CPD C +ETCC + DG +GCCP +A CC D+ CCP CDL ++C P + K +S
Sbjct: 786 MREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCT---QGSSSMPMVQR-LPALKRE-KDASNVVCPGGQSECPDGN----------------TCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSSIASLQ----MHAALQAVTYIKAVDGV--LCPDKKYQCPNGSTCCRVPSGTYGCCPIKNAVCCSDHIHCCPNGYTCDVEGGKCIKNAEHIKMIANVPSSRSSVDTEQKYIKCPD-GCSCLDYETCCVLSDGRFGCCPLPHAVCCEDHEHCCPSGYRCDLAAEMCVTGNVRLPMLQKIRS 1104 HSP 9 Score: 116.701 bits (291), Expect = 1.287e-23 Identity = 84/261 (32.18%), Postives = 122/261 (46.74%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ 984
VCP +++C + TCC Y CC PNA+CC D HCCP+G TCD + C + + L+ +H ++ D + CP+ K +C + TCC G Y CCP KNAVCCSD +CCP CD++ KC+ ++ AN+ + S ++ ++ KCP+ C + CC +S G FGCC +CC D + CCP CD A + CV+ + L + R Q
Sbjct: 874 VCPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSS--------------SIASLQ----MHAALQAVTYIKAVDGVLCPDKKYQCPNGSTCCRVPSGTYGCCPIKNAVCCSDHIHCCPNGYTCDVEGGKCIKNAEHIKMI-------ANVPSSRSSVDTEQKYIKCPDG-CSCLDYETCCVLSDGRFGCCPLPHAVCCEDHEHCCPSGYRCDLAAEMCVTGNVRLPMLQKIRSPRKQ 1108
BLAST of Granulin vs. TrEMBL
Match: W4Z754 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 213.386 bits (542), Expect = 6.564e-56 Identity = 143/423 (33.81%), Postives = 195/423 (46.10%), Query Frame = 1
Query: 142 DGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSK 1401
+ + + + S + + +CP + +C + TCC Y CC PNA+CC D HCCPSG TCD + CT+ E + W +E +S T + + CP + +C D TCC G Y CCP NAVCCSD +CCP CD+ C +V Y+ + SA S++ CP+ +CP CC ++ G +GCC +CC D CCP CD + C S + QI+ + +CPD EC TCC + G YGCCP NA CCSD CCP CD+ C E+ P KS S Y E V+CPD Q++C + + CC++ QYGCC N VCC+D CCP+G C SG C++
Sbjct: 1593 ESVVPWYEKSSATPVQVDSVICPGGQAQCPDGNTCCKLPSGQYGCCPLPNAVCCSDGEHCCPSGYTCDVSAGTCTQG-ESVVLW----------------------YEKSSATPVQVESVVCPGGQAQCPDGNTCCKLPSGQYGCCPLPNAVCCSDGEHCCPSGYTCDVSAGTCTQGESVVPWYEKS---SATPVQIESVV--------CPDGQSECPDGSTCCKLASGQYGCCPIPSAVCCSDHLHCCPSGYTCDVSAGTCTQGESVVPWYEKSSATPVQIE-SVVCPDGQSECPDGSTCCKLASGQYGCCPLPNAVCCSDGQHCCPSGYTCDVSAGTCTQGESVVPWYEKS-SATPVYVE---SVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTR 1976 HSP 2 Score: 209.149 bits (531), Expect = 2.219e-54 Identity = 140/418 (33.49%), Postives = 196/418 (46.89%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + K+S V E+N VCP+ +++C + TCC Y CC PNA+CC D HCCPSG TC + CT+ E + W +E +S T + +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + S V CP+ +CP CC ++ G +GCC +CC D CCP C + C S + ++ N +CPD EC TCC++ G YGCCP NA CCSD+ CCP C + C E+ P KS + +H V+CPD Q++C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 595 VPWYEKSSATPVHEENV-VCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQG-ESVVPW----------------------YEKSSATPVLVENVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRGESVVLWYEKS-----------SATPVQVENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-NVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-----NVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSG 971 HSP 3 Score: 204.912 bits (520), Expect = 5.474e-53 Identity = 138/436 (31.65%), Postives = 197/436 (45.18%), Query Frame = 1
Query: 175 DQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKFHE 1470
D S + +CP+ +++C + TCC Y CC PNA+CC D HCCPSG TC + CT+ E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + ++ + CP+ +CP CC ++ G +GCC +CC D CCP C + C S + ++ + +CPD EC TCC + G YGCCP NA CCSD+ CCP C + C E+ P KS + +H S V+CPD Q++C + + CC + QYGCC N VCC+D CCP+G C SG C++ + + Y T HE
Sbjct: 141 DLTSNASAVICPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQG-ESVVPW----------------------YEKSSATPVHVENVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVESVV-----------CPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRGESVVLWYEKSSATPVHVE-SVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVES-----VVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVP-----WYEKSSATPVHE 531 HSP 4 Score: 204.527 bits (519), Expect = 7.757e-53 Identity = 137/419 (32.70%), Postives = 189/419 (45.11%), Query Frame = 1
Query: 142 DGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + S+ + S + +CP + +C + TCC Y CC PNA+CC D HCCPSG TC + CTR E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + +A S+L CP +CP CC ++ G +GCC +CC D CCP C + C S + ++ N +CPD EC TCC + G YGCCP NA CCSD+ CCP C + C E+ P KS + VICP Q +C + + CC++ QYGCC N VCC+D + CCP+G C VS G
Sbjct: 1285 ESVVSWYEKSAATPVQVESVICPGGQAQCPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSAGTCTRG-ESVVLW----------------------YEKSSATPVQVENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVLWYEKS---AATPVQVESVL--------CPGGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-NVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVQVD----SVICPGGQAQCPDGNTCCKLPSGQYGCCPLPNAVCCSDGEHCCPSGYTCDVSAG 1664 HSP 5 Score: 204.527 bits (519), Expect = 7.905e-53 Identity = 137/417 (32.85%), Postives = 191/417 (45.80%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + K+S V +N VCP+ +++C + TCC Y CC PNA+CC D HCCPSG TC + CT+ E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V + Y+ + + + CP QCP CC ++ G +GCC +CC D CCP C + C S + Q++ N +CPD EC TCC++ G YGCCP NA CCSD+ CCP C + C E+ KS + VICP Q++C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 826 VPWYEKSSATPVHVENV-VCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQG-ESVVPW----------------------YEKSSATPVHVENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVSWYEKSAATPVQVKSVI-----------CPGGQAQCPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSAGTCTRGESVVLWYEKSSATPVQVE-NVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVLWFEKSAATPVQVE----SVICPGGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSG 1202 HSP 6 Score: 204.142 bits (518), Expect = 1.142e-52 Identity = 136/417 (32.61%), Postives = 192/417 (46.04%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + K++ V + +CP +++C + TCC Y CC PNA+CC D HCCPSG TC + CT+ E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + + + VD + CP QCP CC + G +GCC +CC D + CCP CD + C S + Q++ + +CP +C TCC + G YGCCP NA CCSD CCP CD+ C E+ P KS + V+CPD Q++C + S CC++ QYGCC + VCC+D CCP+G C VS G
Sbjct: 1442 VLWYEKSAATPV-QVESVLCPGGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQG-ESVVPW----------------------YEKSSATPVHVENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSA---------TPVQVDSV--ICPGGQAQCPDGNTCCKLPSGQYGCCPLPNAVCCSDGEHCCPSGYTCDVSAGTCTQGESVVLWYEKSSATPVQVE-SVVCPGGQAQCPDGNTCCKLPSGQYGCCPLPNAVCCSDGEHCCPSGYTCDVSAGTCTQGESVVPWYEKSSATPVQIE----SVVCPDGQSECPDGSTCCKLASGQYGCCPIPSAVCCSDHLHCCPSGYTCDVSAG 1818 HSP 7 Score: 203.371 bits (516), Expect = 1.696e-52 Identity = 137/420 (32.62%), Postives = 191/420 (45.48%), Query Frame = 1
Query: 142 DGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + + + S VCP+ +++C + TCC Y CC PNA+CC D HCCPSG TC + CTR E + W +E +S T + + CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + ++ + CP+ +CP CC ++ G +GCC +CC D CCP C + C S + E+ + N+ CPD EC TCC + G YGCCP NA CCSD+ CCP C + C E+ P KS S + E V+CPD Q++C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 284 ESVVPWYEKSSATPVHVESVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRG-ESVVLW----------------------YEKSSATPVHVESVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVESVV-----------CPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWY--EKSSATPVHEENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKS-SATPVHEE---NVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSG 663 HSP 8 Score: 201.83 bits (512), Expect = 6.770e-52 Identity = 139/418 (33.25%), Postives = 192/418 (45.93%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK-VICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + K+S V +N VCP+ +++C + TCC Y CC PNA+CC D HCCPSG TC + CTR E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + S V CP+ +CP CC ++ G +GCC +CC D CCP C + C S + ++ N +CPD EC TCC + G YGCCP NA CCSD+ CCP C + C E+ KS + + + +K VICP Q +C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 672 VPWYEKSSATPVLVENV-VCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRG-ESVVLW----------------------YEKSSATPVQVENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKS-----------SATPVHVENVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-NVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVSWYEKSAA-----TPVQVKSVICPGGQAQCPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSAG 1048 HSP 9 Score: 201.445 bits (511), Expect = 7.797e-52 Identity = 134/423 (31.68%), Postives = 192/423 (45.39%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL------KVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ F K++ V + +CP +++C + TCC Y CC PNA+CC D HCCPSG TC + CT+ E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V + Y+ + + + CP QCP CC ++ G +GCC +CC D CCP C + C S + Q++ N +CPD EC TCC + G YGCCP NA CCSD+ CCP C + C T+ +S++ +Y + V+CP Q++C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 1134 VLWFEKSAATPV-QVESVICPGGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQG-ESVVPW----------------------YEKSSATPVHVENVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVSWYEKSAATPVQVESVI-----------CPGGQAQCPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSAGTCTRGESVVLWYEKSSATPVQVE-NVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTC----------TQGESVVLWYEKSAATPVQVESVLCPGGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSG 1510 HSP 10 Score: 199.134 bits (505), Expect = 6.011e-51 Identity = 132/425 (31.06%), Postives = 188/425 (44.24%), Query Frame = 1
Query: 142 DGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL------KVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+ + S+ + S + +CP + +C + TCC Y CC PNA+CC D HCCPSG TC + CTR E + W +E +S T +++ CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V ++ + + + CP +CP CC ++ G +GCC +CC D CCP C + C S + ++ N +CPD EC TCC + G YGCCP NA CCSD+ CCP C + C T+ +S++ +Y + VICP Q +C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 977 ESVVSWYEKSAATPVQVKSVICPGGQAQCPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSAGTCTRG-ESVVLW----------------------YEKSSATPVQVENVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVLWFEKSAATPVQVESVI-----------CPGGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-NVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTC----------TQGESVVSWYEKSAATPVQVESVICPGGQAQCPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSAG 1356 HSP 11 Score: 194.512 bits (493), Expect = 2.102e-49 Identity = 138/436 (31.65%), Postives = 191/436 (43.81%), Query Frame = 1
Query: 94 KVKFMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
K + ++TI++ +L F EN C + C T TCC +N CC YPNA+CC D HCCP+G CD C + N ++ML + SN+ + D +++C D TCC G Y CCP NAVCCSD +CCP C + + C +V Y+ + S V CP+ +CP CC ++ G +GCC +CC D CCP C + C S + ++ + +CPD EC TCC++ G YGCCP NA CCSD+ CCP C + C E+ KS + +H S V+CPD Q++C + + CC++ QYGCC N VCC+D CCP+G C VS G
Sbjct: 42 KREMKLVTILVVASLVSLAFGGPLKVSKVEELAKENTC---KDGCATTSTCCNDLNNESGCCTYPNAVCCSDGRHCCPTGYECDVTSNTCIKG------------NDVMRML-------ITDLTSNASAVICPDG------QSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKS-----------SATPVHVENVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-SVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRGESVVLWYEKSSATPVHVES-----VVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSG 432 HSP 12 Score: 165.236 bits (417), Expect = 1.577e-39 Identity = 139/479 (29.02%), Postives = 195/479 (40.71%), Query Frame = 1
Query: 142 DGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLN-------NICPDKNVECQSFETCCSV-GDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK------------------------VICP-DQTKCENDSVCCQMDDNQYGCCSYKNGV----------CCADKQTCCPNGTQCVSG-----GCSKVK*SIKCLKLI 1434
+ + + + S + VCP+ +++C + TCC Y CC P+A+CC D HCCPSG TCD + CT+ E + W +E +S T + + CP+ +++C D TCC G Y CCP NAVCCSD +CCP CD+ C +V Y+ + SA S++ CP+ +CP CC ++ G +GCC +CC D CCP C + C SS L R KL +ICPD C S TCC + G +GCCP NA CC D+ CCP C C+ P ++KS LL E + VICP + +C++ + CC + Y CC V C D+ + CP G+ C GC + +I C LI
Sbjct: 1747 ESVVPWYEKSSATPVQIESVVCPDGQSECPDGSTCCKLASGQYGCCPIPSAVCCSDHLHCCPSGYTCDVSAGTCTQG-ESVVPW----------------------YEKSSATPVQIESVVCPDGQSECPDGSTCCKLASGQYGCCPLPNAVCCSDGQHCCPSGYTCDVSAGTCTQGESVVPWYEKS---SATPVYVESVV--------CPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRGSSILEWSSKSSARSNVDKLQITATEPVSICPDSKTSCPSSSTCCPLMASGEWGCCPVHNAVCCDDHIHCCPSGYSCGTNS--CQKGSMHLPLLSKSTGLLKLTFEGQEEPKPEVKVHDNKHLLFQPDEPRTGSVICPGGKIECQDGNACCLLAGGDYSCCPLPQAVSPHEESGDKMMCPDEISSCPTGSTCCQTPGNIYGCCPYQAAICCSDLI 2189 HSP 13 Score: 164.081 bits (414), Expect = 3.318e-39 Identity = 135/456 (29.61%), Postives = 192/456 (42.11%), Query Frame = 1
Query: 142 DGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLE-------KKCPNSDKQCPGQKKCCP-MSGGHFGCCKFNKGICCPDMKTCCPHNTDCD-------SAKKACVSKSSDLTEIPNERQREA--QIKLNN----------------ICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQ-TKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVS--GGCSK 1401
+ + + + S + VCP+ +++C + TCC Y CC PNA+CC D HCCPSG TCD + CT+ E + W +E +S T + + CP+ +++C D TCC G Y CCP NAVCCSD +CCP C + + C S++ + +S NVDKL+ CP+S CP CCP M+ G +GCC + +CC D CCP C S +SKS+ L ++ E Q E ++K+++ ICP +ECQ CC + G Y CCP A + + E G K++CPD+ + C S CCQ N YGCC Y+ +CC+D CCP+ C S G C+K
Sbjct: 1824 ESVVPWYEKSSATPVQIESVVCPDGQSECPDGSTCCKLASGQYGCCPLPNAVCCSDGQHCCPSGYTCDVSAGTCTQG-ESVVPW----------------------YEKSSATPVYVESVVCPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRGSSILEWSSKSSARS----------NVDKLQITATEPVSICPDSKTSCPSSSTCCPLMASGEWGCCPVHNAVCCDDHIHCCPSGYSCGTNSCQKGSMHLPLLSKSTGLLKLTFEGQEEPKPEVKVHDNKHLLFQPDEPRTGSVICPGGKIECQDGNACCLLAGGDYSCCPLPQA---------------------------------------VSPHEESGDKMMCPDEISSCPTGSTCCQTPGNIYGCCPYQAAICCSDLIHCCPSDYSCNSSTGTCTK 2207 HSP 14 Score: 159.844 bits (403), Expect = 9.823e-38 Identity = 97/302 (32.12%), Postives = 138/302 (45.70%), Query Frame = 1
Query: 574 CCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSL-LHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKFH 1467
CC + NAVCCSD +CCP +CD+ + C+ + V + + L + CP+ +CP CC ++ G +GCC +CC D CCP C + C S + ++ N +CPD EC TCC++ G YGCCP NA CCSD+ CCP C + C E+ P KS + +H S V+CPD Q++C + + CC + QYGCC N VCC+D CCP+G C SG C++ + ++ Y T H
Sbjct: 99 CCTYPNAVCCSDGRHCCPTGYECDVTSNTCIKGNDV-------------MRMLITDLTSNASAVICPDGQSECPDGNTCCKLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVE-NVVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTQGESVVPWYEKSSATPVHVES-----VVCPDGQSECPDGNTCCTLASGQYGCCPLPNAVCCSDHLHCCPSGYTCGVSSGTCTRGE-----SVVLWYEKSSATPVH 376
BLAST of Granulin vs. TrEMBL
Match: A0A2B4RQD3 (Granulin (Fragment) OS=Stylophora pistillata OX=50429 GN=GRN PE=4 SV=1) HSP 1 Score: 204.912 bits (520), Expect = 5.248e-55 Identity = 135/415 (32.53%), Postives = 192/415 (46.27%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLI 1434
+CP +++C + TCC + CC P A+CC D HCCPSG+TCD A C RN + K LK + G S T+ + + ++C D TCC G Y CCP AVCCSD +CCP C L C S+ ++ K + CP+ + +CP CC +S G +GCC K +CC D + CCP+ CD + C ++ L + + + N++ CPD +C TCC + G YGCCP A CCSD CCP+ CD+ C +S K ++ +E ++CPD +++C +D+ CC++ QYGCC VCC+D + CCPNG C G CSK SI L+ +
Sbjct: 108 ICPGGKSECPDGNTCCKLSTGGWGCCPLPEAVCCSDGVHCCPSGTTCDLATGTCDRNTD--------------KAFLLKKTQVWIGKAGKSNTICPDGE-------SQCPDGNTCCKLSSGQYGCCPLPKAVCCSDGKHCCPNGYTCHLSAGTCTQGSSSIAMFQKKPAKKRESAASNIV---------CPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDISAGTCSKGTTTLILLQKLPAVQRKSDANSVVCPDGESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPNGYTCDVSAGTCTQGSSSIAMFQKKPAMKRESAES--NIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSPGTCSKGTTSITFLQKL 490 HSP 2 Score: 201.83 bits (512), Expect = 6.126e-54 Identity = 144/432 (33.33%), Postives = 196/432 (45.37%), Query Frame = 1
Query: 139 TDGIFSFNKASEDQV----SEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKK-------CPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNE-RQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
TD F K QV + K+ +CP+ ++C + TCC Y CC P A+CC D HCCP+G TC + CT+ + M KP+ SN I CP+ +++C D TCC G Y CCP AVCCSD +CCP CD+ +AG S T L + +++K CP+ + QCP CC + G +GCC K +CC D K CCP+ CD + C SS + + + + N +CPD EC TCC + G YGCCP A CCSD CCP+ CD+ C TS + K ++ V+CPD ++C + + CC++ QYGCC N VCC+D++ CCPNG C VS G
Sbjct: 165 TDKAFLLKKT---QVWIGKAGKSNTICPDGESQCPDGNTCCKLSSGQYGCCPLPKAVCCSDGKHCCPNGYTCHLSAGTCTQGSSS------------IAMFQKKPAKKRESAASN---------IVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDI----------------SAGTCSKGTTTLILLQKLPAVQRKSDANSVVCPDGESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPNGYTCDVSAGTCTQGSSSIAMFQKKPAMKRESAESNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSPGTCSKGTTSITFLQKLPAVQR--KSDANSVVCPDGDSQCPDGTTCCKLSTGQYGCCPLPNAVCCSDREHCCPNGYTCDVSAG 554 HSP 3 Score: 174.096 bits (440), Expect = 8.667e-44 Identity = 134/405 (33.09%), Postives = 183/405 (45.19%), Query Frame = 1
Query: 289 NAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEW----PVVEENLPVKMLDLKPSSTLHG-----------FESNSETLRVNDD-------------------IXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
A+CCPDK HCCP G TC K C+ + P + ++LDL H E S + R + D + CP K++C D TCC G + CCP AVCCSD +CCP T CDL C N+ K LK ++ + K CP+ + QCP CC +S G +GCC K +CC D K CCP+ C + C SS + + ++ + +NI CPD EC TCC + G YGCCP A CCSD CCP+ CD+ C +K T+ + + + S+ V+CPD +++C + + CC++ QYGCC VCC+D + CCPNG C VS G
Sbjct: 3 RAVCCPDKIHCCPQGYTCSKDGKTCSSGSDTVASLTSTSPKALKKPLERLLDLIKGRYSHKKTLLLKHSTVKMEKISASKRRDHDKTPPIKKTPALRNDSKVEIVICPGGKSECPDGNTCCKLSTGGWGCCPLPEAVCCSDGVHCCPSGTTCDLATGTC-----DRNTDKAFLLKKTQVW----IGKAGKSNTICPDGESQCPDGNTCCKLSSGQYGCCPLPKAVCCSDGKHCCPNGYTCHLSAGTCTQGSSSIAMFQKKPAKKRESAASNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDISAGTC-SKGTTTLILLQKLPAVQRKSDAN-SVVCPDGESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPNGYTCDVSAG 396 HSP 4 Score: 171.785 bits (434), Expect = 5.217e-43 Identity = 116/379 (30.61%), Postives = 181/379 (47.76%), Query Frame = 1
Query: 163 KASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQM 1293
K ++ + S + VCP+ ++C + TCC Y CC P A+CC D HCCP+G TCD + C++ ++ + LP +S++ ++ D +++C D TCC G Y CCP AVCCSD +CCP CD+ C S+ ++ K + + N+ CP+ + +CP CC +S G +GCC K +CC D + CCP+ CD + C ++ +T + + + N++ CPD + +C TCC + G YGCCP NA CCSD CCP+ CD+ C +S P + K +L+ S V+CPD Q++C + + CC++
Sbjct: 253 KPAKKRESAASNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDISAGTCSKGTT----TLILLQKLPAVQ-----------RKSDANSVVCPDG------ESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPNGYTCDVSAGTCTQGSSSIAMFQ----KKPAMKRESAESNI-----VCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSPGTCSKGTTSITFLQKLPAVQRKSDANSVVCPDGDSQCPDGTTCCKLSTGQYGCCPLPNAVCCSDREHCCPNGYTCDVSAGSCTQGSSSIPMVQKLPALMREKS--ASNVVCPDGQSECPDGNTCCKL 599 HSP 5 Score: 139.043 bits (349), Expect = 1.940e-31 Identity = 97/323 (30.03%), Postives = 134/323 (41.49%), Query Frame = 1
Query: 586 KNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSA-------------------NLYTFYSLLNVDKLEKK---------------------------CPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREA--QIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPD-QTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSK 1401
+ AVCC DK +CCP+ C K C S S + K+ L +S + ++K+ CP +CP CC +S G +GCC + +CC D CCP T CD A C +++D + + Q K N ICPD +C TCC + G YGCCP A CCSD CCP+ C L C +S K + ++CPD +++C +D+ CC++ QYGCC VCC+D + CCPNG C +G CSK
Sbjct: 2 QRAVCCPDKIHCCPQGYTCSKDGKTCSSGSDTVASLTSTSPKALKKPLERLLDLIKGRYSHKKTLLLKHSTVKMEKISASKRRDHDKTPPIKKTPALRNDSKVEIVICPGGKSECPDGNTCCKLSTGGWGCCPLPEAVCCSDGVHCCPSGTTCDLATGTC-DRNTDKAFLLKKTQVWIGKAGKSNTICPDGESQCPDGNTCCKLSSGQYGCCPLPKAVCCSDGKHCCPNGYTCHLSAGTCTQGSSSIAMFQKKPA--KKRESAASNIVCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDISAGTCSK 321 HSP 6 Score: 80.4925 bits (197), Expect = 3.133e-12 Identity = 69/225 (30.67%), Postives = 95/225 (42.22%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMS 822
+F A + + +E N VCP+ ++C + TCC Y CC P A+CC D HCCP+G TCD + C++ + L P+ ++NS D +C D TCC G Y CCP NAVCCSD+ +CCP CD+ C S+ + K L S NV CP+ +CP CC +S
Sbjct: 407 MFQKKPAMKRESAESNI-VCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSPGTCSKGTTS------------ITFLQKLPA-VQRKSDANSVVCPDGDS--------QCPDGTTCCKLSTGQYGCCPLPNAVCCSDREHCCPNGYTCDVSAGSCTQGSSSIPMVQ----KLPALMREKSASNV-----VCPDGQSECPDGNTCCKLS 600
BLAST of Granulin vs. TrEMBL
Match: A0A369S146 (Granulins-like protein OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_007797 PE=4 SV=1) HSP 1 Score: 202.216 bits (513), Expect = 6.717e-55 Identity = 124/416 (29.81%), Postives = 186/416 (44.71%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKL-NNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSG 1389
+ + K + VCP+ +++C TCC++ +Y CC +PNA CC DK HCCPSG TCD + CT GP++ P+ D K ++ + D + T C + G+Y CCP+ NA CCSD +CCP CD+ C + + K + CP+ +CP CC + G +GCC + +CC D + CCP C+ C + + ++ ++ + N +C D EC + TCC + G YGCCP+ NATCCSDN CCP C++ C P +TK + S +CP Q++C + + CC++ YGCC + N CC+D + CCP+G C +G
Sbjct: 78 VITMRKKIPATPAPAKNGVCPDGQSECPTGTTCCISASGAYGCCPFPNASCCSDKEHCCPSGYTCDVSAGTCTM---GPLQLPMYAR------FDAKKNNKKVKVSQPASNGVCPDGTSECPVGTTCCKSA------SGSYGCCPYPNATCCSDMEHCCPSGFTCDVSGGTCQMGTVKLPMFAKLSPKK---------VKAPAANGVCPDGISECPSGTTCCKLVSGSYGCCPYPNAVCCSDHEHCCPSGYTCNVGAGTCRAGNIEVPMFAKFSVKKVKTPASNGVCADGQSECPAGTTCCKLASGSYGCCPYPNATCCSDNEHCCPGGYTCNVGAGTCSMGTIKLPMLTKIPVMKRAPSP--KNGMCPGGQSECPSGTTCCKLGSGSYGCCPFPNATCCSDMEHCCPSGFTCNTG 467 HSP 2 Score: 182.185 bits (461), Expect = 2.243e-47 Identity = 130/425 (30.59%), Postives = 179/425 (42.12%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQI-KLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILYSTFEFTKFHECP 1476
CP +++C TCC Y CC +PNA CC D HCCP G TC+ C V+ K + P+ +G + ++ +C TCCI+ G Y CCP NA CCSDK +CCP CD+ C Y K N S + + CP+ +CP CC + G +GCC + CC DM+ CCP CD + C + L ++ + N +CPD EC S TCC + G YGCCP+ NA CCSD+ CCP C++ C+ P K S+ + V Q++C + CC++ YGCC Y N CC+D + CCP G C G S+ +KL + + K P
Sbjct: 20 CPGGQSECPAGTTCCNLGSGQYGCCPFPNATCCADHEHCCPGGYTCNTGAGTCQVGTT------VITMR---KKIPATPAPAKNGVCPDGQS--------------ECPTGTTCCISASGAYGCCPFPNASCCSDKEHCCPSGYTCDVSAGTCTMGPLQLPMYARFDAKKNNKKVKVSQPASNGV---CPDGTSECPVGTTCCKSASGSYGCCPYPNATCCSDMEHCCPSGFTCDVSGGTCQMGTVKLPMFAKLSPKKVKAPAANGVCPDGISECPSGTTCCKLVSGSYGCCPYPNAVCCSDHEHCCPSGYTCNVGAGTCRAGNIEVPMFAK-FSVKKVKTPASNGVCADGQSECPAGTTCCKLASGSYGCCPYPNATCCSDNEHCCPGGYTCNVG---AGTCSMGTIKLPMLTKIPVMKRAPSP 414 HSP 3 Score: 159.458 bits (402), Expect = 3.681e-39 Identity = 110/341 (32.26%), Postives = 150/341 (43.99%), Query Frame = 1
Query: 190 KNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKK--CPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---VSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITK 1197
+ VCP+ ++C TCC + SY CC YPNA CC D HCCPSG TCD + C G ++ P+ + P K+ ++ + D I+ + T C G+Y CCP+ NAVCCSD +CCP C++ C AG ++ +S+ V C + +CP CC ++ G +GCC + CC D + CCP C+ C K LT+IP + A N +CP EC S TCC +G G YGCCPF NATCCSD CCP C+ C + P +
Sbjct: 177 ASNGVCPDGTSECPVGTTCCKSASGSYGCCPYPNATCCSDMEHCCPSGFTCDVSGGTCQM---GTVKLPMFAKLSPKKV------------KAPAANGVCPDGISECPSGTTCCKLV------SGSYGCCPYPNAVCCSDHEHCCPSGYTCNVGAGTC-----------RAGNIEVPMFAKFSVKKVKTPASNGVCADGQSECPAGTTCCKLASGSYGCCPYPNATCCSDNEHCCPGGYTCNVGAGTCSMGTIKLPMLTKIP--VMKRAPSPKNGMCPGGQSECPSGTTCCKLGSGSYGCCPFPNATCCSDMEHCCPSGFTCNTGAGTCSLGKIELPMFAR 483
BLAST of Granulin vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 180.644 bits (457), Expect = 8.284e-49 Identity = 127/410 (30.98%), Postives = 183/410 (44.63%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC---TRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAV-------FNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLN--NICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+CP+ C + TCC T Y CC PNA CC D HCC G+ CD +C T ++ M P+ + +LP ++ S ++ ++ D G++ CCP NAVCC DK +CCP+ T CDL + KC+ S F +YK A K+ L + + ++ CP+ CP CCP+ G +GCC K +CC D CCP T CD A CVS S+ L + ++K+ ++ +++V C+S TCC DG + CCP A CC D+ CCP VC V + + K ++ + + C T C + CC+M+ +GCC VCC D CCP T C V+ G
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQAVVCPDQESEC---PDDTTCCQLPD----------------------GSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVD--CPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVS-SNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVASADLLTLVRWVEKKPTINRVPQPANE-KCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAG 398 HSP 2 Score: 176.407 bits (446), Expect = 2.178e-47 Identity = 131/415 (31.57%), Postives = 189/415 (45.54%), Query Frame = 1
Query: 178 QVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICP-------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNK------ETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
Q S VCP+ ++C + TCC S+ CC PNA+CC DK HCCP G+TCD +C ++ G + P+ + K + K +SN L ++D+ CP+ + C D TCC +G+Y CCP AVCCSD +CCP+ T CDL + KC+ S+ + N ++ V + P N C CC G + CC + +CC D CCP T C A+ CV+ + LT + R E + +N + P D C TCC + G +GCCP A CC D+ CCP N VC++ C + + S P + K+ + S C + + C + CC+ + CC VCC D + CCP G +C
Sbjct: 88 QPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSV--PLRRKFPAYKRAEWKARK-----DSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTN----------LSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCVASADLLTLV---RWVEKKPTINRV-PQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPAT----SLPPANDQCDETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKC 475 HSP 3 Score: 163.31 bits (412), Expect = 7.758e-43 Identity = 131/465 (28.17%), Postives = 194/465 (41.72%), Query Frame = 1
Query: 97 VKFMILTIIIDYALTDGIFSFNKASEDQ----VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC-TRNVEGPMEW---PVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQ-------CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---VSKSSDLTEIPNERQREAQI--KLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKN---------------KETSQPSITKSKSLLH-FYSEIGLKVICPDQTKCENDSVCCQMDD-NQYGCCSYKNGVCCADKQTCCPNGTQC 1380
V M+ ++ + + KA +D +++ N+ CP+ + C + TCC + SY CC P A+CC D HCCP G++CD A +C + NV P+ PVV + V+ + P + ES S + D G++ CCP AVCC D +CCP+ T C ++ + SA+L T + + P + CP CC M G +GCC + +CC D CCP NT C+ A C S ++ +P R+ A N+ C D+ C + TCC G + CCP A CC D+ CCP CD+ + C + + +Q S +K++ +++ K C QT C D+ CC M N++GCC VCC D CCPN C
Sbjct: 150 VSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVESV---PCNESVACESGSTCCKTKD----------------------GDWACCPLPEAVCCEDHIHCCPQGTVC----------------ARSTCVASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSLPPANDQC-DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTC 572 HSP 4 Score: 162.155 bits (409), Expect = 1.890e-42 Identity = 103/310 (33.23%), Postives = 139/310 (44.84%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQ----REAQIKL------------NNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILY 1440
G Y CCP NA CCSD +CC E T CDL++ KC++ + + ++ +L CP+ + +CP CC + G +GCC +CC D CCP T CD CVS S L +P R+ + A+ K N++ CPDK C TCC +G+G YGCCP A CCSD+ CCP CDL C + +P T ++ + V C + CE+ S CC+ D + CC VCC D CCP GT C C S L L+ +
Sbjct: 38 GGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQAVV----------CPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVS--SMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVE-SVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVA---SADLLTLVRW 331 HSP 5 Score: 161.77 bits (408), Expect = 2.692e-42 Identity = 126/434 (29.03%), Postives = 188/434 (43.32%), Query Frame = 1
Query: 136 LTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC----LDSSAVFNSYKNAGLKS------ANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK--------LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
LT + K + ++V + C + T C + TCC + ++ CC P A+CC D HCCP + C+ A C G L V + P+++L + C TCC K G + CCP AVCC D +CCP+ KCD+ ++ C L S F K L+S AN D K ++ CP CC M +GCC + +CC D CCP++ CD+ K C+ EIP +++EA++ + ++ D C + TCC + G +GCCP A CC D+ CCP CDL+ C T T + +L+H +++ +V+C + C CC++ D ++ CC +K VCC D + CCP G C
Sbjct: 326 LTLVRWVEKKPTINRVPQPANEKC-DPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSG------TAGQLSVPWVRKTPATSLPPANDQCDETSA------------CPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFK--KQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGH---LEIPWFKKQEAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLH---TVALTLVHTHTK--EQVMCDATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVC 730 HSP 6 Score: 119.398 bits (298), Expect = 4.476e-28 Identity = 94/326 (28.83%), Postives = 131/326 (40.18%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS----DLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKET 1176
+ + C TCC K ++ CC P A+CC D HCCP G CD + C M W + P + S+ + S V C + +D C + K + CCP AVCC D +CCP + CD CL +K K T S + + +C +S +CP CC ++ G +GCC + +CC D CCP CD C+ S+ LT + + + D C +TCC + D + CCPFK A CC D CCP VCD + K C +
Sbjct: 432 DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLW---FKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAK----VTQGSEVAHGVADVQC-DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLVHTHTKEQVMC-------DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARATS 742
BLAST of Granulin vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 180.644 bits (457), Expect = 8.284e-49 Identity = 127/410 (30.98%), Postives = 183/410 (44.63%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC---TRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAV-------FNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLN--NICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC-VSGG 1392
+CP+ C + TCC T Y CC PNA CC D HCC G+ CD +C T ++ M P+ + +LP ++ S ++ ++ D G++ CCP NAVCC DK +CCP+ T CDL + KC+ S F +YK A K+ L + + ++ CP+ CP CCP+ G +GCC K +CC D CCP T CD A CVS S+ L + ++K+ ++ +++V C+S TCC DG + CCP A CC D+ CCP VC V + + K ++ + + C T C + CC+M+ +GCC VCC D CCP T C V+ G
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQAVVCPDQESEC---PDDTTCCQLPD----------------------GSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVD--CPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVS-SNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVASADLLTLVRWVEKKPTINRVPQPANE-KCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAG 398 HSP 2 Score: 176.407 bits (446), Expect = 2.178e-47 Identity = 131/415 (31.57%), Postives = 189/415 (45.54%), Query Frame = 1
Query: 178 QVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICP-------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNK------ETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
Q S VCP+ ++C + TCC S+ CC PNA+CC DK HCCP G+TCD +C ++ G + P+ + K + K +SN L ++D+ CP+ + C D TCC +G+Y CCP AVCCSD +CCP+ T CDL + KC+ S+ + N ++ V + P N C CC G + CC + +CC D CCP T C A+ CV+ + LT + R E + +N + P D C TCC + G +GCCP A CC D+ CCP N VC++ C + + S P + K+ + S C + + C + CC+ + CC VCC D + CCP G +C
Sbjct: 88 QPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSV--PLRRKFPAYKRAEWKARK-----DSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTN----------LSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCVASADLLTLV---RWVEKKPTINRV-PQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPAT----SLPPANDQCDETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKC 475 HSP 3 Score: 163.31 bits (412), Expect = 7.758e-43 Identity = 131/465 (28.17%), Postives = 194/465 (41.72%), Query Frame = 1
Query: 97 VKFMILTIIIDYALTDGIFSFNKASEDQ----VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC-TRNVEGPMEW---PVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQ-------CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC---VSKSSDLTEIPNERQREAQI--KLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKN---------------KETSQPSITKSKSLLH-FYSEIGLKVICPDQTKCENDSVCCQMDD-NQYGCCSYKNGVCCADKQTCCPNGTQC 1380
V M+ ++ + + KA +D +++ N+ CP+ + C + TCC + SY CC P A+CC D HCCP G++CD A +C + NV P+ PVV + V+ + P + ES S + D G++ CCP AVCC D +CCP+ T C ++ + SA+L T + + P + CP CC M G +GCC + +CC D CCP NT C+ A C S ++ +P R+ A N+ C D+ C + TCC G + CCP A CC D+ CCP CD+ + C + + +Q S +K++ +++ K C QT C D+ CC M N++GCC VCC D CCPN C
Sbjct: 150 VSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVESV---PCNESVACESGSTCCKTKD----------------------GDWACCPLPEAVCCEDHIHCCPQGTVC----------------ARSTCVASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSLPPANDQC-DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTC 572 HSP 4 Score: 162.155 bits (409), Expect = 1.890e-42 Identity = 103/310 (33.23%), Postives = 139/310 (44.84%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQ----REAQIKL------------NNI-CPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLKLILY 1440
G Y CCP NA CCSD +CC E T CDL++ KC++ + + ++ +L CP+ + +CP CC + G +GCC +CC D CCP T CD CVS S L +P R+ + A+ K N++ CPDK C TCC +G+G YGCCP A CCSD+ CCP CDL C + +P T ++ + V C + CE+ S CC+ D + CC VCC D CCP GT C C S L L+ +
Sbjct: 38 GGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQAVV----------CPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVS--SMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVE-SVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVA---SADLLTLVRW 331 HSP 5 Score: 161.77 bits (408), Expect = 2.692e-42 Identity = 126/434 (29.03%), Postives = 188/434 (43.32%), Query Frame = 1
Query: 136 LTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC----LDSSAVFNSYKNAGLKS------ANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSG-GHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIK--------LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
LT + K + ++V + C + T C + TCC + ++ CC P A+CC D HCCP + C+ A C G L V + P+++L + C TCC K G + CCP AVCC D +CCP+ KCD+ ++ C L S F K L+S AN D K ++ CP CC M +GCC + +CC D CCP++ CD+ K C+ EIP +++EA++ + ++ D C + TCC + G +GCCP A CC D+ CCP CDL+ C T T + +L+H +++ +V+C + C CC++ D ++ CC +K VCC D + CCP G C
Sbjct: 326 LTLVRWVEKKPTINRVPQPANEKC-DPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSG------TAGQLSVPWVRKTPATSLPPANDQCDETSA------------CPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFK--KQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGH---LEIPWFKKQEAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLH---TVALTLVHTHTK--EQVMCDATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVC 730 HSP 6 Score: 119.398 bits (298), Expect = 4.476e-28 Identity = 94/326 (28.83%), Postives = 131/326 (40.18%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSS----DLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKET 1176
+ + C TCC K ++ CC P A+CC D HCCP G CD + C M W + P + S+ + S V C + +D C + K + CCP AVCC D +CCP + CD CL +K K T S + + +C +S +CP CC ++ G +GCC + +CC D CCP CD C+ S+ LT + + + D C +TCC + D + CCPFK A CC D CCP VCD + K C +
Sbjct: 432 DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLW---FKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAK----VTQGSEVAHGVADVQC-DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLVHTHTKEQVMC-------DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARATS 742
BLAST of Granulin vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 163.696 bits (413), Expect = 5.675e-43 Identity = 128/427 (29.98%), Postives = 178/427 (41.69%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC--TRNVEGPM--EWPV-----VEENLPVKM---LDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXX-----HGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEI----GLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
V + +CP+ ++C TCC T + CC P A+CC DK HCCP S C +C + N E PM ++P E + PV + L K T +S + N ++ N T G + CCP AVCC D +CCP KC+L KC D S K K + L K+ N CP + CC S G + CC + +CC D CCPH C+ A C S + + P + ++ + K+ N+ + C TCC +G + CCP A CC D CCPH K C++ C++ S P I K S ++ G V C CE++S CC+ D + CC VCC D CCP+G +C
Sbjct: 164 VKKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLK----KVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKC 586 HSP 2 Score: 151.369 bits (381), Expect = 1.160e-38 Identity = 117/402 (29.10%), Postives = 166/402 (41.29%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITK--SKSLLHFYSEIG----LKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
++ C + TCC + CC P A+CC D HCCP G C+ +C + G + W + P++ L P + ++ N +T D+ TCC G + CCP AVCC D +CCP KC+L C D S K K + L K+ N CP + CC S G + CC + +CC D + CCPH C+ A C S + I P ++ ++K + + C+ TCC G + CCP A CC D CCPH K C+L C + S P + K S+ + E G V C C S CC+ + ++ CC + GVCC D+ CCP+ C
Sbjct: 291 DDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKC-EDPSGSVPWLKKVPSRPIREKKL-PETKVYNVPCN-DTAACPDET-------------TCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMK----KLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATC 672 HSP 3 Score: 145.976 bits (367), Expect = 7.106e-37 Identity = 128/510 (25.10%), Postives = 195/510 (38.24%), Query Frame = 1
Query: 103 FMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCC-VTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP--------------NERQREAQIKLNNICP---------------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIG----LKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKFHECPLFLV 1488
+L +++ AL G+ + + CPN + C + TCC V Y+CC +P CC D HCCP+G C A+ +C +N + W E P + K + +N + + D+ + C+ Y CCP + C+D+ +CCP + +C + C+ + CP+ +CP Q CCP + G +GCC + +CC D CCP N+ C CVS ++ E+P N R KL N P D V C TCC G+G + CCP A CC D CCPH K C+L C++ S P + K S ++ V C D C +++ CC+ + ++ CC VCC D CCP+G +C +G C S+ +K + + K E ++ V
Sbjct: 3 LTMLKLMVALALL-GLVTGTQ-------------CPNQQL-CGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKC-QNSTHSLSW---TERKPAQAYLAKSFRMISSSTANKDNIICPDN--------SSCPPEFSCLKLTDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAEPV---------------------------MCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNK--ELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNV 456 HSP 4 Score: 124.79 bits (312), Expect = 8.782e-30 Identity = 103/389 (26.48%), Postives = 150/389 (38.56%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT--------EIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKS--------LLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKN 1329
N+ C + TCC + CC P A+CC D HCCP G C+ A C + G + W + P+K L P + ++ N +T D+ TC + G + CCP AVCC D +CCP KC++ C D S K + + L V C N+ C + CC G + CC + +CC D CCPH C+ A C S + I + + + + N+ D + C + TCC + + CCPF CC D CCPH+ C+L C TS P K+ ++ V C D C + + CC M+ ++ N
Sbjct: 375 NDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTC-EDPSGSVPWMKKLPSQPIKGKKL-PETKVYNVPCN-DTAACPDETTCCKDS-------------EGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKV---PSRPISGKKLPKVKGAGVPC-NATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPVKASGPPPPEKKKPKENKANEPVKCDDHHSCPDQTTCCFMNKTMTQSTTHTN 743
BLAST of Granulin vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 159.073 bits (401), Expect = 2.426e-41 Identity = 138/457 (30.20%), Postives = 185/457 (40.48%), Query Frame = 1
Query: 103 FMILTIIIDYALTDGIF------SFNKASEDQVS--EKNENV-CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC--TRNVEGPM--EWPVV----EENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXX----------XXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLL---HFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ T+I + D +F + ++ S D +S +K E V CP+ ++C TCC T + CC P A+CC DK HCCP S C +C + N E PM ++P EN K L K T +S + N + C D TCC G + CCP AVCC D +CCP KC+L KC D S K + D P N CP + CC S G + CC + +CC D CCPH C+ A C S + + + + C D V C TCC G + CCP A CC D CCPH K C++ C++ S P I K S ++ V C CE++S CC+ D + CC GVCC D+ CCP+ C
Sbjct: 62 MLCQTLICSCFVNDLLFLNHCCPNDHECSADSISCVKKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRRKDLTNKQPKTSTALKSTTTNKTRNLPCALFSVCTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKGFDIPFYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKTLPLPPALGSDVPCND-TVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKAGVPCNATFACEDESTCCKNDKGGWSCCPLPEGVCCEDRIHCCPHDATC 517 HSP 2 Score: 141.739 bits (356), Expect = 1.857e-35 Identity = 118/456 (25.88%), Postives = 167/456 (36.62%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCP-NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQP------------------------------------------------SITKSKSLLHFYSEIGLK-----------VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
++ C + TCC + CC P A+CC D HCCP G C+ +C + G + W + +P + + K GF+ + ND C D TCC G + CCP AVCC D +CCP KC+L C D S K +L L P N CP + CC G + CC + +CC D + CCPH C+ A C S + I P ++ ++K C + C+ TCC G + CCP CC D CCPH+ C+L C TS P S K ++ +Y+++ + V C QT C + CC++ ++GCC CC D++ CCP G C
Sbjct: 225 DDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKC-EDPSGSVPW---LKKVPSRPIREKKLPETKGFDIPFYNVPCND-------TAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKK------------TLPLPPALGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKAGVPC-NATFACEDESTCCKNDKGGWSCCPLPEGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPVKASGPPPPEKKKPKENKANEPAVCCEDGEHCCPAQYKCDKTQTSCIKGDVVIPWYNKLAAESSPDHSVDQSAVKCDSQTSCPAGTTCCEISTGKWGCCPLPEATCCPDREHCCPKGYSC 656 HSP 3 Score: 138.658 bits (348), Expect = 2.274e-34 Identity = 135/475 (28.42%), Postives = 194/475 (40.84%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCC-VTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLK---PSSTLHGFESNSET-LRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSS-------AVFNSYKNA-------------------GLKSAN----------LYTFYSLLNVDKLEKKCPNSDK-QCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQ--------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
CPN + C + TCC V Y+CC +P CC D HCCP+G C C N +L L P+ H ++S + ++ + + CP+ ++C TCC T G + CCP AVCC+DK +CCPEN+ C + + KC+ S+ A F + A LKS L++ ++ NV P D C CC G + CC + +CC D CCPH C+ C S L ++P+ RE + I N+ + C TCC +G + CCP A CC D CCPH K C+L C++ S P + K+ L +G V C D C +++ CC+ + CC VCC D Q CCP+G +C +G C S+ +K + K
Sbjct: 19 CPNQQL-CGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTLICSCFVN----------------DLLFLNHCCPND--HECSADSISCVKKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRRKDLTNKQPKTSTALKSTTTNKTRNLPCALFSVCTVYNV-------PCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKGFDIPFYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKT---LPLPPALGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKL 464 HSP 4 Score: 115.161 bits (287), Expect = 1.099e-26 Identity = 97/354 (27.40%), Postives = 132/354 (37.29%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEW-------PVVEENLPVKMLDLKPSSTL-----------------------------------HGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTE-IPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCD 1143
N+ C + TCC ++CC P A+CC D HCCP G C+ A C + G + W P+ + LP + ++T H N TL + + T K + K ++ AVCC D +CCP KCD C+ V Y +S+ ++ VD+ KC +S CP CC +S G +GCC + CCPD + CCP CD ++CV E IP R QI + C ETCC +GCCP A CCSD CCP C+
Sbjct: 391 NDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTC-EDPSGSVPWIKKVPSRPISGKKLPKVKAGVPCNATFACEDESTCCKNDKGGWSCCPLPEGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPVKASGPPPPEKKKPKENKANEPAVCCEDGEHCCPAQYKCDKTQTSCIKGDVVIPWYNKLAAESSPDHS------VDQSAVKC-DSQTSCPAGTTCCEISTGKWGCCPLPEATCCPDREHCCPKGYSCDVNSQSCVKTILLQVETIPLTYIRHLQIPCGG-----GISCSDTETCCKTSKTTWGCCPSPKAVCCSDMKHCCPAGYTCE 731 HSP 5 Score: 88.5817 bits (218), Expect = 4.850e-18 Identity = 84/301 (27.91%), Postives = 119/301 (39.53%), Query Frame = 1
Query: 721 LYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCP-MSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI----PNERQREAQ----IKLNN--ICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC---KNKETSQ----PSITKSKSLLHFYSEIGLK-------------------------------VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKFHE 1470
L +LL + +CPN + C + CC +S G + CC F +G CC D CCP C + +C +DL + PN+ + A +K +CPD EC TCC DG +GCCP A CC+D CCP N VC + C NKE P+ ++ H ++ K V C D C + + CC+ + + CC VCC D CCP+G +C ++G C S+ LK + K E
Sbjct: 4 LMVALALLGL-VTGTQCPN-QQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTLICSCFV--NDLLFLNHCCPNDHECSADSISCVKKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRRKDLTNKQPKTSTALKSTTTNKTRNLPCALFSVCTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPE 300 HSP 6 Score: 60.077 bits (144), Expect = 3.655e-9 Identity = 53/190 (27.89%), Postives = 78/190 (41.05%), Query Frame = 1
Query: 163 KASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFE-SNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC-------LDSSAVFNSYKNAGL 708
++S D +++ C +++T C TCC + CC P A CCPD+ HCCP G +CD + C + + + E +P+ + G S++ET C T + CCP AVCCSD +CCP C+ N C D+ +VF S K L
Sbjct: 598 ESSPDHSVDQSAVKC-DSQTSCPAGTTCCEISTGKWGCCPLPEATCCPDREHCCPKGYSCDVNSQSCVKTIL------LQVETIPLTYIRHLQIPCGGGISCSDTETC---------------------CKTSKTTWGCCPSPKAVCCSDMKHCCPAGYTCE-ANGSCTQATGFNWDNWSVFFSRKKRAL 758
BLAST of Granulin vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 150.984 bits (380), Expect = 1.511e-38 Identity = 117/402 (29.10%), Postives = 164/402 (40.80%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITK--SKSLLHFYSEIG----LKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
++ C + TCC + CC P A+CC D HCCP G C+ A C + G + W + P+K L P + ++ N +T D+ TCC G + CCP AVCC D +CCP KC+L C D S K + L + C N CP + CC G + CC + +CC D + CCPH C+ A C S + I P ++ ++K + + C+ TCC G + CCP A CC D CCPH K C+L C + S P + K S+ + E G V C C S CC+ + ++ CC + GVCC D+ CCP+ C
Sbjct: 291 DDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTC-EDPSGSVPWMKKLPSQPIKGKKL-PETKVYNVPCN-DTAACPDET-------------TCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKV---PSRPIKGKKLPETKGSDVPC-NDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATC 672 HSP 2 Score: 145.976 bits (367), Expect = 7.308e-37 Identity = 127/504 (25.20%), Postives = 192/504 (38.10%), Query Frame = 1
Query: 103 FMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCC-VTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP--------------NERQREAQIKLNNICP---------------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIG----LKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKFHE 1470
+L +++ AL G+ + + CPN + C + TCC V Y+CC +P CC D HCCP+G C A+ +C +N + W E P + K + +N + + D+ + C+ Y CCP + C+D+ +CCP + +C + C+ + CP+ +CP Q CCP + G +GCC + +CC D CCP N+ C CVS ++ E+P N R KL N P D V C TCC G+G + CCP A CC D CCPH K C+L C++ S P + K S ++ V C D C +++ CC+ + ++ CC VCC D CCP+G +C +G C S+ +K + + K E
Sbjct: 3 LTMLKLMVALALL-GLVTGTQ-------------CPNQQL-CGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKC-QNSTHSLSW---TERKPAQAYLAKSFRMISSSTANKDNIICPDN--------SSCPPEFSCLKLTDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAEPV---------------------------MCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNK--ELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKVPSRPIKGKKLPE 450 HSP 3 Score: 133.65 bits (335), Expect = 1.051e-32 Identity = 123/478 (25.73%), Postives = 179/478 (37.45%), Query Frame = 1
Query: 154 SFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSS--AVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQ---------------------------CPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------PNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEI----GLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKVK*SIKCLKLILYSTFEFTKF 1464
SF S + K+ +CP+N + E C+ + Y CC I C D+ HCCP+ C A C + E P+ P PV+ CC T G + CCP AVCC+DK +CCPEN+ C + + KC+ S+ + K A+ + KL K P + C CC G + CC + +CC D CCPH C+ A C S + + P + ++ + K+ N+ + C TCC +G + CCP A CC D CCPH K C+L C++ S P + K S ++ G V C D C +++ CC+ + CC VCC D Q CCP+G +C +G C S+ +K + K
Sbjct: 97 SFRMISS-STANKDNIICPDNSSCPPEFS--CLKLTDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAE-PVMCPDGVSECPVQTT--------------------------------------CCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKVPSRPIKGKKLPETKGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKL 532 HSP 4 Score: 124.405 bits (311), Expect = 1.054e-29 Identity = 105/389 (26.99%), Postives = 151/389 (38.82%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT--------EIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKS--------LLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKN 1329
N+ C + TCC + CC P A+CC D HCCP G C+ A C + G + W + P+K L E+ + ND + CP+ T CK+ G + CCP AVCC D +CCP KC++ C D S K + + L V C N+ C + CC G + CC + +CC D CCPH C+ A C S + I + + + + N+ D + C + TCC + + CCPF CC D CCPH+ C+L C TS P K+ ++ V C D C + + CC M+ ++ N
Sbjct: 375 NDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTC-EDPSGSVPWMKKVPSRPIKGKKLP--------ETKGSDVPCNDTVACPDETTCCKNV-------KGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKV---PSRPISGKKLPKVKGAGVPC-NATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPVKASGPPPPEKKKPKENKANEPVKCDDHHSCPDQTTCCFMNKTMTQSTTHTN 743 HSP 5 Score: 85.1149 bits (209), Expect = 5.646e-17 Identity = 72/244 (29.51%), Postives = 97/244 (39.75%), Query Frame = 1
Query: 190 KNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPN----SDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCD 909
K +V N+ C + TCC ++CC P A+CC D HCCP G C+ A C + G + W + P+ L P G N+ T D+ TCC G + CCP AVCC D +CCP KC+L C D S + + N +K + N S CP CC + CC F +G+CC D CCPH+ C+
Sbjct: 452 KGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTC-EDPSGSVPWIKKVPSRPISGKKL-PKVKGAGVPCNA-TFACEDE-------------STCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQ------NKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCN 673
BLAST of Granulin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009302.1 (pep scaffold:Pmarinus_7.0:GL483232:1183:11161:1 gene:ENSPMAG00000008413.1 transcript:ENSPMAT00000009302.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 162.54 bits (410), Expect = 2.937e-43 Identity = 120/413 (29.06%), Postives = 180/413 (43.58%), Query Frame = 1
Query: 151 FSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDD--IXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSS-AVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+S + E N +C ++++ C + TCC T + CC A+CC D HCCP G+TC+ + C + +LP + + + LRV + + ++++ C D TCC G++ CCP AVCC D +CCPE T C+L ++C + ++ S K L+ A ++ D+ CP CC + G +GCC K +CC D + CCP T C+ + C + L + N + D C +TCC + G +GCCP A CC D+ CCP C+L + C S P TK+ +L +K C DQ C + + CC+ +GCC VCC D + CCP GT C
Sbjct: 274 WSTKTPALRVAGEPNVVIC-DDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTL----------SLP--------------WSTKTPALRVAGEPNVVICDDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTLSLPWSTKTPALRVAGEPNV--VICDDQFS---------CPDGNTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVICDDQFSCPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRVAGEPNDVK--CDDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTC 648 HSP 2 Score: 161.77 bits (408), Expect = 5.311e-43 Identity = 119/420 (28.33%), Postives = 180/420 (42.86%), Query Frame = 1
Query: 151 FSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDD--IXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSS-AVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEI-------GLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+S + E N +C ++++ C + TCC + CC P A+CC D HCCP G+TC+ +RC + +LP + + + LRV + + +++ C D TCC G++ CCP AVCC D +CCP+ T C+L + C + ++ S K L+ A ++ D+ CP CC M G +GCC K +CC D + CCP T C+ + C + L + N++ D V C TCC G G +GCCP A CC D+ CCP C++ C + S P +K+ + + + G V C C + + CCQ+ Q+GCC CC+D CCP+G C
Sbjct: 352 WSTKTPALRVAGEPNVVIC-DDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTL----------SLP--------------WSTKTPALRVAGEPNVVICDDQFSCPDGNTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNV--VICDDQFS---------CPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRVAGEPNDVKCDDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSCDQGQLSLPWSSKTPARTRLTALLPASAAARGGSVRCDSAYACPDGTTCCQLPQGQWGCCPLPLATCCSDFVHCCPSGYTC 735 HSP 3 Score: 159.458 bits (402), Expect = 3.541e-42 Identity = 121/429 (28.21%), Postives = 168/429 (39.16%), Query Frame = 1
Query: 94 KVKFMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
K K + + T + + A V CP+ T C + TCC Y CC +A+CC D HCCP + CD +C V G + P V + + S V D + G + CCP AVCC DK +CCPE T C++ ++ C + A + + + + + + D L CP CC GG + CC K +CC D + CCP T C+ + C + L + N + D C TCC G + CCP A CC D+ CCP C+L + C S P TK+ +L E + VIC DQ+ C + + CC+M +GCC VCC D Q CCP GT C
Sbjct: 27 KGKISVPWLEKTPTFTPRTWRWRLARSLAVRPPQ---CPDG-TSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKC---VHGKLSIPWVSK-----------------YPSKQAASDVQCDAFTSCSDQNTCCILPS-----GQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTC--NHAALSLPWSTKTPALRMTGEPNDVQCDDLVG--------CPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVICDDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPAL-RVAGEPNV-VICDDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTC 414 HSP 4 Score: 154.836 bits (390), Expect = 1.591e-40 Identity = 120/371 (32.35%), Postives = 160/371 (43.13%), Query Frame = 1
Query: 289 NAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESN-SETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPG------QKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
A+CC D HCCPSG+ CD A C + + V L+ P+ T + + +L V + T C D TCC G Y CC ++AVCCSD +CCP+NT CDLK KC ++ S+ V K K SD QC Q CC + G +GCC K +CC D + CCP T C+ + C + L + N++ D V C TCC G + CCP A CC D+ CCP C+L + C S P TK+ + L E + VIC DQ+ C + + CC+ + CC VCC D + CCP GT C
Sbjct: 1 QAVCCSDGLHCCPSGTVCDLAHGMCQKG------------KISVPWLEKTPTFTPRTWRWRLARSLAVRP--PQCPDGTSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKC-------------------VHGKLSIPWVSKYPSKQAASDVQCDAFTSCSDQNTCCILPSGQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALSLPWSTKTPALRMTGEPNDVQCDDLVGCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPA-LRVAGEPNV-VICDDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTC 336 HSP 5 Score: 123.25 bits (308), Expect = 6.181e-30 Identity = 101/355 (28.45%), Postives = 149/355 (41.97%), Query Frame = 1
Query: 151 FSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDD--IXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSS-AVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICP------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNK 1170
+S + E N +C +++ C + TCC + CC A+CC D HCCP G+TC+ + C + +LP + + + LRV + + +++ C D TCC G++ CCP AVCC D +CCP+ T C+L + C + ++ S K L+ A + + KC + CP Q CC G +GCC K +CC D + CCP T C+ +C +P + A+ +L + P D C TCC + G +GCCP ATCCSD CCP CDL + C K
Sbjct: 430 WSTKTPALRVAGEPNVVIC-DDQFSCPDGNTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTL----------SLP--------------WSTKTPALRVAGEPNVVICDDQFSCPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRVAG----------EPNDVKC-DDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSC---DQGQLSLPWSSKTPARTRLTALLPASAAARGGSVRCDSAYACPDGTTCCQLPQGQWGCCPLPLATCCSDFVHCCPSGYTCDLAQASCIKK 745 HSP 6 Score: 90.8929 bits (224), Expect = 1.592e-19 Identity = 72/270 (26.67%), Postives = 111/270 (41.11%), Query Frame = 1
Query: 151 FSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP 960
+S + E N +C +++ C + TCC + CC A+CC D HCCP G+TC+ + C + L + P+ + G ++ ++ +D ++CP+ T CK G++ CCP AVCC D +CCPE T C++ C D + + + L + +S CP CC + G +GCC CC D CCP CD A+ +C+ K LT P
Sbjct: 508 WSTKTPALRVAGEPNVVIC-DDQFSCPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGA------------LSLPWSTKTPALRVAGEPND---VKCDDQVSCPDQTTCCKTGT-------GDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSC-DQGQLSLPWSSKTPARTRLTALLPASAAARGGSVRCDSAYACPDGTTCCQLPQGQWGCCPLPLATCCSDFVHCCPSGYTCDLAQASCIKKPHFLTWAP 753
BLAST of Granulin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001039.1 (pep scaffold:Pmarinus_7.0:GL484091:11570:24665:-1 gene:ENSPMAG00000000936.1 transcript:ENSPMAT00000001039.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 126.716 bits (317), Expect = 1.970e-31 Identity = 104/386 (26.94%), Postives = 161/386 (41.71%), Query Frame = 1
Query: 226 CKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCV 1383
C+ QTCC ++ + CC A CC D HCCPS + C + N + W LK + L G V D++ C+ +TCC ++ G + CC + A CCSD +CCP T+C + +C++S+A+ K S + NV + C + CC G +GCC + CC D CCP D K CV+ ++ + + E + N + C+ ++TCC G++GCC + ATCCSD CCP C + ++C + + S +L H + C CC+ +GCC+ + CC+D CCP+ T+C+
Sbjct: 227 CRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ---CVNSAALIPW-------------LKKEAALSGP--------VGDNLVHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVG--QCVNSAALIPWLKKEAALSGPVGD-----NVVHC-----GGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGD-LKGQCVNSAALIPWLKKEAALSGPVGDNLVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSGVAGSSTLSHALPY-------DSRVYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCL 568 HSP 2 Score: 125.946 bits (315), Expect = 4.123e-31 Identity = 105/384 (27.34%), Postives = 163/384 (42.45%), Query Frame = 1
Query: 226 CKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGT 1374
C+ QTCC ++ + CC A CC D HCCPS + C + C+R+ E + +LD G+ ++ C+ +TCC ++ G + CC + A CCSD +CCP T+C + +C++S+A+ K S + NV + C + CC G +GCC + CC D CCP T C + CV+ ++ + + E + N + C+ ++TCC G++GCC + ATCCSD CCP C + + C N P + K +L +G V+ C C CC+ +GCC+ + CC+D CCP+G
Sbjct: 74 CRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSE------------ALSVLDYLSREQQDGY-----------NVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVG--QCVNSAALIPWLKKEAALSGPVGD-----NVVHC-----GGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ--CVNSAALIPWLKKEAALSGPVGDNLVHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQ--CVNSAALIPWLKKEAAL---SGPVGDNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGA 415 HSP 3 Score: 119.783 bits (299), Expect = 4.711e-29 Identity = 107/396 (27.02%), Postives = 162/396 (40.91%), Query Frame = 1
Query: 244 CCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK*SIKCLK 1428
CC ++ + CC A CC D HCCPS + C + N + W LK + L G V D++ C+ +TCC ++ G + CC + A CCSD +CCP T+C + + C SS + + + S D + C + CC G +GCC + CC D CCP T C + CV+ ++ + + E + N + C+ ++TCC G++GCC + ATCCSD CCP C + + C N P + K +L +G ++ C C CC+ +GCC+ + CC+D CCP+ T+C+ G C I LK
Sbjct: 1 CCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSAALIPW-------------LKKEAALSGP--------VGDNLVHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSEALS-----------VLDYLSREQQDGYNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ--CVNSAALIPWLKKEAALSGPVGDNVVHCGGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ--CVNSAALIPWLKKEAAL---SGPVGDNLVHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLK 357 HSP 4 Score: 107.457 bits (267), Expect = 4.962e-25 Identity = 92/328 (28.05%), Postives = 135/328 (41.16%), Query Frame = 1
Query: 226 CKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICP-DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKS 1206
C+ QTCC ++ + CC A CC D HCCPS + C + N + W LK + L G V D++ C+ +TCC ++ G + CC + A CCSD +CCP DLK + C++S+A+ K S + D L + C + CC G +GCC + CC D CCP T C + C S L+ + L++ P D V C+ ++TCC G++GCC + ATCCSD CCP C + ++C + + S
Sbjct: 303 CRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQ---CVNSAALIPW-------------LKKEAALSGP--------VGDNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKGQ-CVNSAALIPWLKKEAALSGPVG--------DNLVH--CGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSGVAGSS------TLSHALPYDSRVYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSGVAGRIS 589
BLAST of Granulin vs. Ensembl Nematostella
Match: EDO27824 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T870]) HSP 1 Score: 90.8929 bits (224), Expect = 6.861e-21 Identity = 65/215 (30.23%), Postives = 85/215 (39.53%), Query Frame = 1
Query: 775 NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQT-------------KCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
N C + CC +S G +GCC +CC D K CCP CD + C + D T + + + + I C + ETCC VG YGCCP +A CC+D CCP C+ + C+ P K S +H + + D T CE CC Q CC N CC+D CCP G C
Sbjct: 10 NDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTC-DMAKDSTPMVSYHATVQRAGVGEIKCKDGSHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPS-VHPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFIC 222 HSP 2 Score: 87.8113 bits (216), Expect = 8.405e-20 Identity = 48/129 (37.21%), Postives = 66/129 (51.16%), Query Frame = 1
Query: 994 NNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
N I + C +ETCC + G YGCCP NA CCSD CCP CD+ C + S P ++ ++ + +G ++ C D + C CC + +YGCC + VCCAD Q CCP+G C
Sbjct: 5 NYITCNDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKDSTPMVSYHATVQR--AGVG-EIKCKDGSHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTC 130 HSP 3 Score: 85.1149 bits (209), Expect = 7.664e-19 Identity = 64/218 (29.36%), Postives = 90/218 (41.28%), Query Frame = 1
Query: 562 GNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKL-EKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI---PNERQREAQIKLNNICPDKNVE------------CQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKN 1167
G+Y CCP NAVCCSD +CCP CD+ + C + S + ++++ + + E KC + C + CC + +GCC +CC DM+ CCP C+ C L P+ R A K I D + C+ + TCC CCP NA+CCSD CCP +CD +K K
Sbjct: 27 GSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTC-----------DMAKDSTPMVSYHATVQRAGVGEIKCKDG-SHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPSVHPRPAVAKTPVII-DHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICDDQKGCVKQ 231 HSP 4 Score: 73.559 bits (179), Expect = 7.224e-15 Identity = 65/247 (26.32%), Postives = 92/247 (37.25%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNS-ETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDK----QCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSK 936
N+ + C + +TCC SY CC PNA+CC D HCCP G CD + C + P+V + V+ + G N+ ET + Y CCP +AVCC+D +CCP C+ K+ C V ++ + + +D + P + C CC S CC CC D CCP CD +K CV +
Sbjct: 10 NDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKD---STPMVSYHATVQRAGVGEIKCKDGSHCNTGETCCLVGPT---------------------KYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPSVHPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICDD-QKGCVKQ 231
BLAST of Granulin vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 181.8 bits (460), Expect = 1.129e-48 Identity = 137/422 (32.46%), Postives = 193/422 (45.73%), Query Frame = 1
Query: 136 LTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVD-KLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ + + SF+K VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + G L S NV K+E + CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ +K+ ++ D + C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S++ +K V C T C + + CC+ D +GCC VCC D + CCP GT C
Sbjct: 356 MLEKVPSFSK-----VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------NVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----------EGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSN--VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTC 735 HSP 2 Score: 171.785 bits (434), Expect = 2.762e-45 Identity = 129/418 (30.86%), Postives = 185/418 (44.26%), Query Frame = 1
Query: 148 IFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVD-KLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ +F + S +++ N C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + G L S NV K+E + CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ +K+ ++ D + C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S + +K V C T C + + CC+ + +GCC + VCC D CCP G C
Sbjct: 276 VPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------KVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----------EGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSN--VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTC 656 HSP 3 Score: 164.081 bits (414), Expect = 9.051e-43 Identity = 124/423 (29.31%), Postives = 184/423 (43.50%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEG--PM----------EWP-----------VVEENLPVKMLDLKPSSTLHGF-ESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+C + ++C + TCC + + CC P A+CC DK HCCP +TCD + +C + + PM +W VE N ++ ++ + F E +S + N C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + +S ++V + C S CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ +K+ ++ D + C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S + +K V C T C + + CC+ + +GCC + VCC D CCP G C
Sbjct: 170 LCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC--------EGDGGSVPMLEKVPSFSKVSVKMEDVPCDGS-TSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSN--VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTC 577 HSP 4 Score: 155.606 bits (392), Expect = 7.800e-40 Identity = 136/440 (30.91%), Postives = 180/440 (40.91%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPN--------------------------SDKQCPGQKKCCPMSGGH-FGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL---TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLL--HFYSEIG-LKVI---CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC TK G + CCP AVCC D +CCP T CDL CL +S + K + L+ + ++ N S QCP CC M +GCC K +CC D + CCP + C +CV + T+IP +A +C +CC + G +GCCP NA CC D CCP CDL K C T Q + S+ G +K I C D C D CC+ +GCC VCC D + CCP+G +C GG
Sbjct: 603 VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------KVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQC--SAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRCTKGG 1019 HSP 5 Score: 155.221 bits (391), Expect = 8.020e-40 Identity = 131/439 (29.84%), Postives = 182/439 (41.46%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVD-KLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKET------SQPSITKSKSLLHFYSEIGLK----------------------------VICPDQTKCENDSVCCQMDDNQ-YGCCSYKNGVCCADKQTCCPNGTQC 1380
VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + G L S NV K+E + CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ + +K+ ++ D + C TCC DG +GCCP A CC D+ CCP CDL C P+ T++ S + G + + C T+C + CC M Q +GCC VCCAD + CCP +C
Sbjct: 445 VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------NVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----------EGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSK--VSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKC 850 HSP 6 Score: 140.198 bits (352), Expect = 8.745e-35 Identity = 121/443 (27.31%), Postives = 173/443 (39.05%), Query Frame = 1
Query: 199 NVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL----------TEIPNERQREA-----------------QIKLNNICP-------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
++CPN KC++ QTCC + Y CC + A CC D HCCP CD C N M W VE + K + + E D+I ++ + + +T++G CCP V C D +CCPE +C L ++ C +K ++ T C + +CP + CC G + CC K +CC D CCP T CD + C+S S T E Q+E ++ N P ++ C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S++ +K V C T C + + CC+ + +GCC + VCC D CCP G C
Sbjct: 18 DMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSC-GNATLSMRW--VERVSANLQMATKSFRMIRSYMGEDE-----DNICPDQSRCPAEFSCLKALTRYG---CCPLAQGVSCPDGKHCCPEGHQCSLDSRSC--------------VKKESIKTVL-----------CKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTC 419 HSP 7 Score: 117.472 bits (293), Expect = 2.972e-27 Identity = 104/363 (28.65%), Postives = 159/363 (43.80%), Query Frame = 1
Query: 136 LTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLH-----------------GFESNSETLRVNDD--IXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVS------KSSDLTEI--PNERQREAQIK-LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVC 1140
+ + + SF+K VS K E+V + T C + TCC TK ++ CC P A+CC D HCCP+G+TCD + C + G + + + LP +S L G E E + +++ I+C ++ + T C + + + CCP AVCC+D +CCP + KC ++ C+ V Y + + D +C ++ QCP CC + G +GCC +CC D + CCP CD A K+C ++ LT + P+++ + +K + N C D C ETCC +GCC A CC D CCP C
Sbjct: 672 MLEKVPSFSK-----VSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLK-ASGLVP---MSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDS--------IQADSSFVQC-SAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRC-DDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC 1015 HSP 8 Score: 77.411 bits (189), Expect = 1.754e-14 Identity = 48/142 (33.80%), Postives = 68/142 (47.89%), Query Frame = 1
Query: 985 IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSI----------TKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ ++CP+ +C+ +TCC Y CCPF A CC D+ CCP + +CD+ C N S + TKS ++ Y ICPDQ++C + C + +YGCC GV C D + CCP G QC
Sbjct: 14 LVAADMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSCLKA-LTRYGCCPLAQGVSCPDGKHCCPEGHQC 153 HSP 9 Score: 73.559 bits (179), Expect = 2.936e-13 Identity = 58/172 (33.72%), Postives = 79/172 (45.93%), Query Frame = 1
Query: 223 KCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYS 738
+C E +CC + CC PNA+CC DK HCCP G TCD A K C + + + E +P+ + L + HG L DD+ C +TCC T H + CC AVCC D +CCP+ +C K C+ KN GL N +TF++
Sbjct: 890 QCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLT------LQLETVPLTPVFLPDDQSQHG--PVKPILNRCDDV------YSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCI---------KNTGLHWLNWHTFFT 1037
BLAST of Granulin vs. Ensembl Medaka
Match: grnb (granulin precursor [Source:NCBI gene;Acc:101166910]) HSP 1 Score: 179.874 bits (455), Expect = 1.427e-48 Identity = 120/399 (30.08%), Postives = 177/399 (44.36%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTE-----IPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPS-ITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+CP+ ++C + TCC S+ CC +P A+CC D+ HCCP G+TCD +C P ++ ++ + + TL F + + + CP K+ C ++ TCC+ G++ CCP+ A CCSD +CCP NT CDL+ C A + ++ CP+ CP CC ++ G FGCC +CC D CCP T CD A CVS + E + + ++ + + +V C TCC G +GCCP A CC D+ CCPH VC+L C + S + + + + SE G C + C ++ CC+ + CC VCC D CCPN T C
Sbjct: 50 ICPDQESECPDETTCCQLPDGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCAS--------PSLQAFPMLQKVPAQRVGTLPVFAAAAS-------VVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVCKSGEARVEPIRAVSASPEDVL--------------CPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAVPCNASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGR---CDESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATIC 416 HSP 2 Score: 169.088 bits (427), Expect = 1.046e-44 Identity = 127/399 (31.83%), Postives = 176/399 (44.11%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKS-SDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPS---ITKSKSLLHFYSE--IGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
VCP ++ C + TCC+ + CC YP A CC D HCCP +TCD + C ++ E +E P++ + P +D+ CP+ ++ C D TCC +G + CCP NAVCCSD +CCPE T CDL + C+ ++ + T V L C N+ C CC S G +GCC + +CC D CCPH T C+ C S S LT + + N C D++ C TCC G + CCP A CC D+ CCP+ +C+LE + C + P +TK L E G +CP +T CC+ Q+ CC VCC+D CCP G +C
Sbjct: 135 VCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVC-KSGEARVE--------PIRAVSASP-----------------EDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTH----PVRGLAVPC-NASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGRC-DESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRLTAGMEEEKCGKDAVCPGRT------TCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKC 495 HSP 3 Score: 162.155 bits (409), Expect = 2.411e-42 Identity = 126/412 (30.58%), Postives = 171/412 (41.50%), Query Frame = 1
Query: 181 VSEKNENV-CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVV------EENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIP--NERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSITKSKSLLHFYSEIG-LKVICPDQTKCENDSVCCQMDDN-QYGCCSYKNGVCCADKQTCCPNGTQC 1380
VS E+V CP+ R+ C + TCC + ++ CC PNA+CC D HCCP G+TCD A C G E +V + PV+ L + P + S R + G + CCP AVCC D +CCP T C+L C D S S L + + + S +C + CPG+ CC G + CC + +CC D CCP+ T C+ + C S +P +R R C K+ C TCC G + CCP A CCSD CCP C+ + C + S P K +L + G ++ C QT C D+ CC M+ ++GCC VCC D CCP G C
Sbjct: 201 VSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAV-PCNASVACADGSTCCRAS----------------------RGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPS---GSALTPLLSNTPGFPYRS------ENGRC-DESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRLTAGMEEEKC-GKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQQRSGQTGPVENKCDAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSC 578 HSP 4 Score: 159.073 bits (401), Expect = 2.629e-41 Identity = 118/396 (29.80%), Postives = 162/396 (40.91%), Query Frame = 1
Query: 211 NNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD----LTEIPNERQREAQI-KLNNICPDKNVECQSFETCCSVGDGL-YGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
N C + TCC + CC P A+CC D HCCP G+ C+ C + G P++ S GF SE R ++ +CP T C+ G + CCP AVCC D +CCP T C+L+ + C D S + T L E+KC D CPG+ CC S G + CC + +CC D+ CCP C++ + C ++P +QR Q + N C D C TCC + L +GCCP A CC D CCP C + C P TK +L +K C +++ C + + CC + +GCC VCCAD + CCP C
Sbjct: 295 NASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTC-DDPSGSALTPLL--------------SNTPGFPYRSENGRCDESASCPGEATCCRTRA-------GGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLP--------WLTKRPRLTAGMEEEKC-GKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQQRSGQTGPVENKC-DAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRGSHVVPWFTKVGALTEPSGVTDVK--CDNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTC 656 HSP 5 Score: 136.732 bits (343), Expect = 9.352e-34 Identity = 111/410 (27.07%), Postives = 169/410 (41.22%), Query Frame = 1
Query: 190 KNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRC--TRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGG-HFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVIC--PDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCS 1398
++EN + C TCC T+ + CC P A+CC D HCCP+ + C+ + C + P+ W L M E + D CP T CK++ G + CCP AVCCSD +CCP+ KC+ ++ C ++ K L +E KC ++ CP CC M +GCC + +CC D CCP C + +C S + + ++ D C S TCC + G +GCCP A CC+D+ CCP + C+++ C+ K +++ ++ + V C + KC + CC++ +GCC VCC D + CCP G C +GGC+
Sbjct: 366 RSENGRCDESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRLTAGM----------------EEEKCGKDAVCPGRTTCCKNSS-------GQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQ---KLPALQQRSG--QTGPVENKC-DAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRGSHVVPWFTKVGALTEPSGVTDVKCDNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEKKAGQGLPLSR---VVPAQPQDTQDVPCDGTGEFKCPKQNTCCRISATAWGCCPSPGAVCCMDSKHCCPAGYSCDLKAGGCT 743 HSP 6 Score: 87.0409 bits (214), Expect = 1.181e-17 Identity = 56/172 (32.56%), Postives = 74/172 (43.02%), Query Frame = 1
Query: 886 CPHNTDCDSAKKACVSKSSDLT-EIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQ-PSITKSKS----LLHFYSEIGLKVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
CP C K ++ T +P+ R ICPD+ EC TCC + DG +GCCPF A CC D CCP CDL C + P + K + L ++ V+CP ++ C N CC + +GCC Y CC+D CCP T C
Sbjct: 17 CPDGAVCADGHKCFLNSHGGFTCPLPHTRVGAV------ICPDQESECPDETTCCQLPDGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCASPSLQAFPMLQKVPAQRVGTLPVFAAAA-SVVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTC 181
BLAST of Granulin vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 177.948 bits (450), Expect = 1.976e-47 Identity = 131/406 (32.27%), Postives = 185/406 (45.57%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + +S ++V + C S CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ +K+ ++ D + C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S++ +K V C T C + + CC+ D +GCC VCC D + CCP GT C
Sbjct: 445 VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------NVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC--------EGDGGSVPMLEKVPSFSKVSVKMEDVPCDGS-TSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSN--VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTC 814 HSP 2 Score: 177.178 bits (448), Expect = 4.139e-47 Identity = 135/422 (31.99%), Postives = 192/422 (45.50%), Query Frame = 1
Query: 136 LTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVD-KLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ + + SF+K VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + G L S NV K+E + CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ + +K+ ++ D + C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S + +K V C T C + + CC+ + +GCC + VCC D CCP G C
Sbjct: 356 MLEKVPSFSK-----VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------NVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----------EGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSK--VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTC 735 HSP 3 Score: 163.696 bits (413), Expect = 1.248e-42 Identity = 124/423 (29.31%), Postives = 184/423 (43.50%), Query Frame = 1
Query: 202 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEG--PM----------EWP-----------VVEENLPVKMLDLKPSSTLHGF-ESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+C + ++C + TCC + + CC P A+CC DK HCCP +TCD + +C + + PM +W VE N ++ ++ + F E +S + N C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + +S ++V + C S CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ +K+ ++ D + C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S + +K V C T C + + CC+ + +GCC + VCC D CCP G C
Sbjct: 170 LCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC--------EGDGGSVPMLEKVPSFSKVSVKMEDVPCDGS-TSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSN--VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTC 577 HSP 4 Score: 154.836 bits (390), Expect = 1.229e-39 Identity = 131/439 (29.84%), Postives = 182/439 (41.46%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVD-KLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSD---LTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKET------SQPSITKSKSLLHFYSEIGLK----------------------------VICPDQTKCENDSVCCQMDDNQ-YGCCSYKNGVCCADKQTCCPNGTQC 1380
VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC T+ G + CCP AVCC D +CCP+ C+L + C + G L S NV K+E + CP Q CC G +GCC F + +CC D CCP C+ A + C L ++P+ + +K+ ++ D + C TCC DG +GCCP A CC D+ CCP CDL C P+ T++ S + G + + C T+C + CC M Q +GCC VCCAD + CCP +C
Sbjct: 524 VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------KVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----------EGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSK--VSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKC 929 HSP 5 Score: 154.836 bits (390), Expect = 1.287e-39 Identity = 136/440 (30.91%), Postives = 180/440 (40.91%), Query Frame = 1
Query: 181 VSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPN--------------------------SDKQCPGQKKCCPMSGGH-FGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL---TEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLL--HFYSEIG-LKVI---CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGG 1392
VS K E+V + T C + TCC T+ + CC +P A+CC D HCCP G TC+ A + C EG + V ML+ PS + + V + + T C D TCC TK G + CCP AVCC D +CCP T CDL CL +S + K + L+ + ++ N S QCP CC M +GCC K +CC D + CCP + C +CV + T+IP +A +C +CC + G +GCCP NA CC D CCP CDL K C T Q + S+ G +K I C D C D CC+ +GCC VCC D + CCP+G +C GG
Sbjct: 682 VSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC----EG--------DGGSVPMLEKVPSFS---------KVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQC--SAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRCTKGG 1098 HSP 6 Score: 139.428 bits (350), Expect = 1.571e-34 Identity = 121/443 (27.31%), Postives = 173/443 (39.05%), Query Frame = 1
Query: 199 NVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDL----------TEIPNERQREA-----------------QIKLNNICP-------------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLK---VICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
++CPN KC++ QTCC + Y CC + A CC D HCCP CD C N M W VE + K + + E D+I ++ + + +T++G CCP V C D +CCPE +C L ++ C +K ++ T C + +CP + CC G + CC K +CC D CCP T CD + C+S S T E Q+E ++ N P ++ C TCC +G +GCCPF A CC D CCP K C+L + C+ S P + K S +S++ +K V C T C + + CC+ + +GCC + VCC D CCP G C
Sbjct: 18 DMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSC-GNATLSMRW--VERVSANLQMATKSFRMIRSYMGEDE-----DNICPDQSRCPAEFSCLKALTRYG---CCPLAQGVSCPDGKHCCPEGHQCSLDSRSC--------------VKKESIKTVL-----------CKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPS----FSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTC 419 HSP 7 Score: 117.087 bits (292), Expect = 4.205e-27 Identity = 104/363 (28.65%), Postives = 159/363 (43.80%), Query Frame = 1
Query: 136 LTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLH-----------------GFESNSETLRVNDD--IXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVS------KSSDLTEI--PNERQREAQIK-LNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVC 1140
+ + + SF+K VS K E+V + T C + TCC TK ++ CC P A+CC D HCCP+G+TCD + C + G + + + LP +S L G E E + +++ I+C ++ + T C + + + CCP AVCC+D +CCP + KC ++ C+ V Y + + D +C ++ QCP CC + G +GCC +CC D + CCP CD A K+C ++ LT + P+++ + +K + N C D C ETCC +GCC A CC D CCP C
Sbjct: 751 MLEKVPSFSK-----VSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLK-ASGLVP---MSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDS--------IQADSSFVQC-SAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRC-DDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC 1094 HSP 8 Score: 77.0258 bits (188), Expect = 2.300e-14 Identity = 48/142 (33.80%), Postives = 68/142 (47.89%), Query Frame = 1
Query: 985 IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSI----------TKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ ++CP+ +C+ +TCC Y CCPF A CC D+ CCP + +CD+ C N S + TKS ++ Y ICPDQ++C + C + +YGCC GV C D + CCP G QC
Sbjct: 14 LVAADMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSCLKA-LTRYGCCPLAQGVSCPDGKHCCPEGHQC 153 HSP 9 Score: 73.1738 bits (178), Expect = 3.313e-13 Identity = 58/172 (33.72%), Postives = 79/172 (45.93%), Query Frame = 1
Query: 223 KCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYS 738
+C E +CC + CC PNA+CC DK HCCP G TCD A K C + + + E +P+ + L + HG L DD+ C +TCC T H + CC AVCC D +CCP+ +C K C+ KN GL N +TF++
Sbjct: 969 QCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLT------LQLETVPLTPVFLPDDQSQHG--PVKPILNRCDDV------YSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCI---------KNTGLHWLNWHTFFT 1116
BLAST of Granulin vs. Ensembl Medaka
Match: ENSORLT00000029765.1 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 91.2781 bits (225), Expect = 4.150e-20 Identity = 74/259 (28.57%), Postives = 107/259 (41.31%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTF-YSLLNVDKLEKKCP-----------------NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC 927
CP+ + C + TCC+T H + CC P A+CC DK HCCPSG C+ A C + + + P++ + + L S+ L N L ++ C D TCC G ++CCP+ CC D ++CCP CDL C+ + Y L S + N +LEK +S CP CC + G + CC + G CC D + CC + CD+ C
Sbjct: 24 CPDGKL-CPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 280 HSP 2 Score: 80.8777 bits (198), Expect = 1.654e-16 Identity = 78/264 (29.55%), Postives = 104/264 (39.39%), Query Frame = 1
Query: 766 KCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ--------------IKLNNICPDKN--VECQSF------ETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-----------KNKETSQP----------SITKSKSLLHFY------SEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKV 1404
+CP+ K CP CC ++G F CC + +CC D CCP C+ A C +P + A+ +K +N K+ V+C SF TCC G + CCP+ CC D CCP+ CDL C K TS P SI KSL SE G+ + C + C + CC+ + Q+ CC Y G CCAD + CC G C S C K+
Sbjct: 23 ECPDG-KLCPDLSTCC-LTGHGFSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGV-IRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCRKL 283 HSP 3 Score: 79.7221 bits (195), Expect = 3.277e-16 Identity = 67/242 (27.69%), Postives = 96/242 (39.67%), Query Frame = 1
Query: 559 HGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSA------NLYT--FYSLLNVDKLEKKCP----NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSA---------------KKACVSKSSDLTEIPNERQREAQIKLNNICP-------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCK 1164
HG + CCP AVCCSDK +CCP +C+L C + + S ++A +L T L N + L +K +S CP + CC G + CC + G CC D CCP+ DCD K+A S + L P E + + L + D C + +CC +G + CCP+ CC+D CC + CD C+
Sbjct: 41 HG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCR 281 HSP 4 Score: 55.8398 bits (133), Expect = 2.967e-8 Identity = 41/170 (24.12%), Postives = 75/170 (44.12%), Query Frame = 1
Query: 172 EDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRN-VEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVND-----DIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC 663
++ +++K+ V ++ C + TCC ++ CC Y CC D +HCCP G CD C R+ + P + ++P ++ +++ +S + +D + ++K C +CC G + CCP+ CC+D +CC CD + C
Sbjct: 111 DNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 280
BLAST of Granulin vs. Ensembl Medaka
Match: ENSORLT00000005071.2 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 91.2781 bits (225), Expect = 4.484e-20 Identity = 74/259 (28.57%), Postives = 107/259 (41.31%), Query Frame = 1
Query: 205 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTF-YSLLNVDKLEKKCP-----------------NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKAC 927
CP+ + C + TCC+T H + CC P A+CC DK HCCPSG C+ A C + + + P++ + + L S+ L N L ++ C D TCC G ++CCP+ CC D ++CCP CDL C+ + Y L S + N +LEK +S CP CC + G + CC + G CC D + CC + CD+ C
Sbjct: 26 CPDGKL-CPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 282 HSP 2 Score: 80.8777 bits (198), Expect = 1.486e-16 Identity = 78/264 (29.55%), Postives = 104/264 (39.39%), Query Frame = 1
Query: 766 KCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQ--------------IKLNNICPDKN--VECQSF------ETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-----------KNKETSQP----------SITKSKSLLHFY------SEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC--VSGGCSKV 1404
+CP+ K CP CC ++G F CC + +CC D CCP C+ A C +P + A+ +K +N K+ V+C SF TCC G + CCP+ CC D CCP+ CDL C K TS P SI KSL SE G+ + C + C + CC+ + Q+ CC Y G CCAD + CC G C S C K+
Sbjct: 25 ECPDG-KLCPDLSTCC-LTGHGFSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGV-IRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCRKL 285 HSP 3 Score: 80.1073 bits (196), Expect = 2.940e-16 Identity = 67/242 (27.69%), Postives = 96/242 (39.67%), Query Frame = 1
Query: 559 HGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSA------NLYT--FYSLLNVDKLEKKCP----NSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSA---------------KKACVSKSSDLTEIPNERQREAQIKLNNICP-------------DKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCK 1164
HG + CCP AVCCSDK +CCP +C+L C + + S ++A +L T L N + L +K +S CP + CC G + CC + G CC D CCP+ DCD K+A S + L P E + + L + D C + +CC +G + CCP+ CC+D CC + CD C+
Sbjct: 43 HG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCR 283 HSP 4 Score: 55.8398 bits (133), Expect = 2.931e-8 Identity = 41/170 (24.12%), Postives = 75/170 (44.12%), Query Frame = 1
Query: 172 EDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRN-VEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVND-----DIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC 663
++ +++K+ V ++ C + TCC ++ CC Y CC D +HCCP G CD C R+ + P + ++P ++ +++ +S + +D + ++K C +CC G + CCP+ CC+D +CC CD + C
Sbjct: 113 DNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 282
BLAST of Granulin vs. Planmine SMEST
Match: SMESG000018770.1 (SMESG000018770.1) HSP 1 Score: 818.15 bits (2112), Expect = 0.000e+0 Identity = 431/438 (98.40%), Postives = 435/438 (99.32%), Query Frame = 1
Query: 94 KVKFMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK 1407
KVKFMILTII+DYALTDGIFSFNKAS+DQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKN GLKSANLYTFYSLLNVDKLEKKCPNS+KQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNE Q EAQIKLNNICPDK+VECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK
Sbjct: 6 KVKFMILTIIVDYALTDGIFSFNKASQDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK 443
BLAST of Granulin vs. Planmine SMEST
Match: SMESG000018770.1 (SMESG000018770.1) HSP 1 Score: 816.609 bits (2108), Expect = 0.000e+0 Identity = 431/438 (98.40%), Postives = 435/438 (99.32%), Query Frame = 1
Query: 94 KVKFMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK 1407
KVKFMILTII+DYALTDGIFSFNKAS+DQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKN GLKSANLYTFYSLLNVDKLEKKCPNS+KQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNE Q EAQIKLNNICPDK+VECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK
Sbjct: 3 KVKFMILTIIVDYALTDGIFSFNKASQDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK 440
BLAST of Granulin vs. Planmine SMEST
Match: SMESG000018770.1 (SMESG000018770.1) HSP 1 Score: 809.675 bits (2090), Expect = 0.000e+0 Identity = 427/434 (98.39%), Postives = 431/434 (99.31%), Query Frame = 1
Query: 106 MILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNERQREAQIKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK 1407
MILTII+DYALTDGIFSFNKAS+DQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKN GLKSANLYTFYSLLNVDKLEKKCPNS+KQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNE Q EAQIKLNNICPDK+VECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK
Sbjct: 1 MILTIIVDYALTDGIFSFNKASQDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVICPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQCVSGGCSKVK 434
BLAST of Granulin vs. Planmine SMEST
Match: SMESG000018767.1 (SMESG000018767.1) HSP 1 Score: 267.7 bits (683), Expect = 2.187e-83 Identity = 191/481 (39.71%), Postives = 251/481 (52.18%), Query Frame = 1
Query: 151 FSFNKASEDQVSEK--NENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLY-----TFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNK-------------------------------------GICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------------PNERQREAQI--KLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLH--FYSE----------IGLKVICPDQTK-CENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+K ED + + +N+CPN RTKC + QTCC TKHNSYHCC+Y NA+CCPDK+HCCP GSTCDA + +C + + +E + +P ++ +P + + + N + D++ CPNNKTKCKDTKTCCITKH NYYCCPHKNAVCCSDKY CCPE TKCDLKNKKCL +S V S N L+ + + + K+EK C + CPG+K CC M G FGCCKFN +CCPD CCP + CD+ + CV + E+ P EA++ K +CP+ +C+ +TCC Y CCP KNA CCSD CCP CDL+ K C + S T ++ H F +E ++ C DQ C CC MD ++GCC + +GVCC D +TCCP+ T+C
Sbjct: 9 LHASKTLEDMLKKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL---IPALKVEDEPKTNILEAKVNEK-----DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTEC 481 HSP 2 Score: 228.024 bits (580), Expect = 2.813e-68 Identity = 140/278 (50.36%), Postives = 178/278 (64.03%), Query Frame = 1
Query: 133 ALTDGIFSFNKASEDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLY-----TFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT 951
+ G F K + V+ +N+CPN RTKC + QTCC TKHNSYHCC+Y NA+CCPDK+HCCP GSTCDA + +C + + +E + +P ++ +P + + + N + D++ CPNNKTKCKDTKTCCITKH NYYCCPHKNAVCCSDKY CCPE TKCDLKNKKCL +S V S N L+ + + + K+EK C + CPG+K CC M G FGCCKFN G+CCPD+KTCCPH T+CD C++ L
Sbjct: 229 VMDAGKFGCCKFNHKNVT--TDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL---IPALKVEDEPKTNILEAKVNEK-----DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCIANRLPLV 496 HSP 3 Score: 135.961 bits (341), Expect = 1.919e-34 Identity = 123/376 (32.71%), Postives = 182/376 (48.40%), Query Frame = 1
Query: 172 EDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAI-------CCPDKYHCCPSGSTCDAAK--KRCTRNVEG-PMEWPVVEEN---LPVKMLDLKPS-----STLHGFES------------NSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCL-------DSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKS------SDLTEIPNERQREAQ---------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCD 1143
E +V+EK+E +CPNN+TKCK+T+TCC+TKHN+Y+CC + NA+ CCP+ C C +A NV+ P + + E++ L K+ ++ T G ++ V D CPN +TKC D +TCC TKH +Y+CC + NAVCC DK++CCPE + CD K KC+ + + + K N+ + +N +K E CPN+ +C K CC ++ CC +CC D CCP T CD K C+S S +++ +P++ Q K+ C D+ C + CC + G +GCC F + CC D +CCPH CD
Sbjct: 110 EAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNI--LEAKVN-EKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECD 482
BLAST of Granulin vs. Planmine SMEST
Match: SMESG000018762.1 (SMESG000018762.1) HSP 1 Score: 267.7 bits (683), Expect = 1.408e-82 Identity = 188/464 (40.52%), Postives = 245/464 (52.80%), Query Frame = 1
Query: 196 ENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYT-----FYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNK-------------------------------------GICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEI------------PNERQREAQI--KLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLH--FYSE----------IGLKVICPDQTK-CENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+N+CPN RTKC + QTCC TKHNSYHCC+Y NA+CCPDK+HCCP GSTCDA + +C + + +E + +P ++ +P + + + N + D++ CPNNKTKCKDTKTCCITKH NYYCCPHKNAVCCSDKY CCPE TKCDLKNKKCL +S V S N L+ + + + K+EK C + CPG+K CC M G FGCCKFN +CCPD CCP + CD+ + CV + E+ P EA++ K +CP+ +C+ +TCC Y CCP KNA CCSD CCP CDL+ K C + S T ++ H F +E ++ C DQ C CC MD ++GCC + +GVCC D +TCCP+ T+C
Sbjct: 92 DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL---IPALKVEDEPKTNILEAKVNEK-----DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTEC 547 HSP 2 Score: 228.409 bits (581), Expect = 1.076e-67 Identity = 136/257 (52.92%), Postives = 171/257 (66.54%), Query Frame = 1
Query: 196 ENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXXXXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNAGLKSANLYT-----FYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLT 951
+N+CPN RTKC + QTCC TKHNSYHCC+Y NA+CCPDK+HCCP GSTCDA + +C + + +E + +P ++ +P + + + N + D++ CPNNKTKCKDTKTCCITKH NYYCCPHKNAVCCSDKY CCPE TKCDLKNKKCL +S V S N L+ + + + K+EK C + CPG+K CC M G FGCCKFN G+CCPD+KTCCPH T+CD C++ L
Sbjct: 314 DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL---IPALKVEDEPKTNILEAKVNEK-----DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCIANRLPLV 562 HSP 3 Score: 105.916 bits (263), Expect = 5.075e-24 Identity = 69/217 (31.80%), Postives = 102/217 (47.00%), Query Frame = 1
Query: 559 HGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCL-------DSSAVFNSYKNAGLKSANLYTFYSLLNVDKLEKKCPNSDKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKS------SDLTEIPNERQREAQ---------IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCD 1143
H +Y+CC + NAVCC DK++CCPE + CD K KC+ + + + K N+ + +N +K E CPN+ +C K CC ++ CC +CC D CCP T CD K C+S S +++ +P++ Q K+ C D+ C + CC + G +GCC F + CC D +CCPH CD
Sbjct: 335 HNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNI--LEAKVN-EKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECD 548 HSP 4 Score: 99.7525 bits (247), Expect = 5.894e-22 Identity = 64/167 (38.32%), Postives = 87/167 (52.10%), Query Frame = 1
Query: 172 EDQVSEKNENVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDIXXXXXXXXXXXXXXXX--XXXHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCL 666
E +V+EK+E +CPNN+TKCK+T+TCC+TKHN+Y+CC + NA+CC DKY CCP G+ CD K+C VVE V D P +SN E + + K + G + CC + VCC D CCP T+CD K +C+
Sbjct: 398 EAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLS------ASFVVESQTNV---DTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCI 555 HSP 5 Score: 86.2705 bits (212), Expect = 1.171e-17 Identity = 52/144 (36.11%), Postives = 70/144 (48.61%), Query Frame = 1
Query: 985 IKLNNICPDKNVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGL-----------KVICP-DQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 1380
+ +NICP+K +C +TCC Y CC + NA CC D CCP CD ++ C S + K L E +V+CP ++TKC++ CC N Y CC +KN VCC+DK CCP GT+C
Sbjct: 89 VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKC 232 The following BLAST results are available for this feature:
BLAST of Granulin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Granulin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of Granulin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Granulin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Granulin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Granulin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Granulin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Granulin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Granulin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Granulin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Granulin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Granulin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Granulin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Granulin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007938 ID=SMED30007938|Name=Granulin|organism=Schmidtea mediterranea sexual|type=transcript|length=1563bpback to top protein sequence of SMED30007938-orf-1 >SMED30007938-orf-1 ID=SMED30007938-orf-1|Name=SMED30007938-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=444bp MLLMLKVKFMILTIIIDYALTDGIFSFNKASEDQVSEKNENVCPNNRTKCback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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