TNF receptor-associated factor

Overview


Name	TNF receptor-associated factor
Smed ID	SMED30007937
Length (bp)	1123

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of TNF receptor-associated factor (SMED30007937) t-SNE clustered cells

Violin plots show distribution of expression levels for TNF receptor-associated factor (SMED30007937) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TNF receptor-associated factor (SMED30007937) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TNF receptor-associated factor (SMED30007937) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30007937

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30007937	SMESG000000368.1	SmedASXL_080827	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30007937	SMESG000000368.1	SmedASXL_080827	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
epidermis	SMED30007937	SMESG000000368.1	dd_Smed_v4_7494_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007937	SMESG000000368.1	Contig22758	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007937	SMESG000000540.1 SMESG000000372.1 SMESG000000371.1	Contig2295	GPL15192	PMID:29674431 Fincher et al., 2018
oocyte	SMED30007937	SMESG000020187.1 SMESG000000368.1 SMESG000036236.1	Contig29941	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30007937	SMESG000020187.1 SMESG000000368.1 SMESG000036236.1	Contig29941	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
oocyte	SMED30007937	SMESG000020187.1 SMESG000000368.1 SMESG000036236.1	Contig29941	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30007937	SMESG000020187.1 SMESG000000368.1 SMESG000036236.1	Contig29941	newmark_ests	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 49.6766 bits (117), Expect = 6.220e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  333 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 417

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 49.6766 bits (117), Expect = 6.719e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  416 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 500

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 49.6766 bits (117), Expect = 6.719e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  416 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 500

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 49.6766 bits (117), Expect = 7.190e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  391 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 475

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 49.6766 bits (117), Expect = 7.190e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  391 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 475

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf3 (TNF receptor-associated factor 3 [Source:ZFIN;Acc:ZDB-GENE-040801-257])

HSP 1 Score: 49.6766 bits (117), Expect = 3.895e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDKWI------YSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G L W+I    + +++  +      YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L+WPFK ++ + L++
Sbjct:  421 GTLIWKIRDYKRRKQEAVVSKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVVMRGEYDALLQWPFKQKVTLMLMD 505

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: BX901915.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_1_18:28764891:28768979:-1 gene:ENSDARG00000109857.1 transcript:ENSDART00000191629.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX901915.1)

HSP 1 Score: 48.521 bits (114), Expect = 4.740e-6
Identity = 30/101 (29.70%), Postives = 49/101 (48.51%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+  W+I   S+  +D        ++S  FY+   GYKMCL + + GD  G              RG    + ++F +MRG  DA L+WPF  ++ + L++
Sbjct:  126 GVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTG--------------RG--THLSLFFVVMRGHSDALLKWPFNQKVTLMLLD 210

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: AL844899.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_2_4:28756846:28760787:-1 gene:ENSDARG00000111038.1 transcript:ENSDART00000183806.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL844899.1)

HSP 1 Score: 48.521 bits (114), Expect = 4.740e-6
Identity = 30/101 (29.70%), Postives = 49/101 (48.51%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+  W+I   S+  +D        ++S  FY+   GYKMCL + + GD  G              RG    + ++F +MRG  DA L+WPF  ++ + L++
Sbjct:  126 GVFVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTG--------------RG--THLSLFFVVMRGHSDALLKWPFNQKVTLMLLD 210

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: hspb6 (heat shock protein family B (small) member 6 [Source:Xenbase;Acc:XB-GENE-876273])

HSP 1 Score: 49.6766 bits (117), Expect = 4.564e-6
Identity = 33/109 (30.28%), Postives = 52/109 (47.71%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDKW------IYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYD 836
            G+  W+I  L+    D        +YS  FY+   GYK+CL + + GD  GA              G    + ++F +M+G+ DA L WPFK+++   L+   D N+ D
Sbjct:  230 GVFLWKISDLTNKSHDAVTGRAISLYSPAFYTAKYGYKVCLRIYLNGD--GA--------------GKGTHVSLFFTVMKGEYDALLPWPFKHKVTFVLV---DHNNRD 319

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: TRAF2 (TNF receptor associated factor 2 [Source:NCBI gene;Acc:100494415])

HSP 1 Score: 49.6766 bits (117), Expect = 5.012e-6
Identity = 31/118 (26.27%), Postives = 58/118 (49.15%), Query Frame = 3

Query:  480 IQSIQTKFGNAEFINF-GILDWQIDKLSQLERD------KWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            +  ++T+   +E   + G+  W+I +  +  +D        ++S PFY+   GYKMCL + + GD  G  R   +S              ++F +M+G+ DA L WPF  ++ + L++
Sbjct:  288 VAELETRMQESELSTYDGVFIWKISEFGKKRQDAINGRCPAMFSPPFYTNKYGYKMCLRIYLNGDGTG--RNTHLS--------------LFFVVMKGNNDALLRWPFNQKVTLMLLD 389

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: TRAF2 (TNF receptor associated factor 2 [Source:NCBI gene;Acc:100494415])

HSP 1 Score: 49.6766 bits (117), Expect = 5.230e-6
Identity = 31/118 (26.27%), Postives = 58/118 (49.15%), Query Frame = 3

Query:  480 IQSIQTKFGNAEFINF-GILDWQIDKLSQLERD------KWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            +  ++T+   +E   + G+  W+I +  +  +D        ++S PFY+   GYKMCL + + GD  G  R   +S              ++F +M+G+ DA L WPF  ++ + L++
Sbjct:  315 VAELETRMQESELSTYDGVFIWKISEFGKKRQDAINGRCPAMFSPPFYTNKYGYKMCLRIYLNGDGTG--RNTHLS--------------LFFVVMKGNNDALLRWPFNQKVTLMLLD 416

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 49.6766 bits (117), Expect = 6.005e-6
Identity = 32/101 (31.68%), Postives = 54/101 (53.47%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++++ L++
Sbjct:  409 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLMD 493

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 49.6766 bits (117), Expect = 6.365e-6
Identity = 32/101 (31.68%), Postives = 54/101 (53.47%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++++ L++
Sbjct:  384 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLMD 468

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 49.2914 bits (116), Expect = 5.167e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  415 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 499

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 49.2914 bits (116), Expect = 5.385e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  390 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 474

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 49.2914 bits (116), Expect = 5.385e-6
Identity = 32/101 (31.68%), Postives = 53/101 (52.48%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G+L W+I    + +++        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  390 GVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMD 474

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 48.1358 bits (113), Expect = 5.933e-6
Identity = 26/108 (24.07%), Postives = 55/108 (50.93%), Query Frame = 3

Query:  531 ILDWQIDKLSQLE-RDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMK 851
            + D+++ K   +E     ++S+PFY+   GY++C    + GD  G              +G    + +YF +MRG+ D+ L+WPF+  + + L++ +   ++  ++ K
Sbjct:  102 VTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSG--------------KGTH--LSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNHIVETFK 193

[Close]

Query:  531 ILDWQIDKLSQLE-RDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMK 851
            + D+++ K   +E     ++S+PFY+   GY++C    + GD  G              +G    + +YF +MRG+ D+ L+WPF+  + + L++ +   ++  ++ K
Sbjct:  102 VTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSG--------------KGTH--LSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNHIVETFK 193

[Close]

BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A1X7U807 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 GN=105313828 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.357e-7
Identity = 35/107 (32.71%), Postives = 59/107 (55.14%), Query Frame = 3

Query:  543 QIDKLSQLERDKWI-YSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYD 860
            ++D+ ++ + +K + YS  FYS PGGYKMCL+V   G+ G A                   I  + HL+ GD D  LEWPFK E+++ L+N  +   ++K+ +++ +
Sbjct:  265 KLDEYTKHKEEKTVWYSPAFYSSPGGYKMCLSVFCNGNGGAANHY----------------ISCFVHLLSGDYDDTLEWPFKGEVSLELLNQMEDKCHEKELLRFSE 355

[Close]

BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A3M7SHF5 (TNF receptor-associated factor 4-like OS=Brachionus plicatilis OX=10195 GN=BpHYR1_034858 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.661e-7
Identity = 54/210 (25.71%), Postives = 100/210 (47.62%), Query Frame = 3

Query:  303 SNPNNKLTTLRNRSGRVEQNPNGKLMHLNKTLNSSRI*K*FIEMIEFVIQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKL------SQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQ---SMKYYDCSDDAR-LGRPAREK 902
            ++ N +  +LR+   R  +   G  +HLN    +  I K  +E++  +I+ + E    +I S+ +K  + E I+   + W+ID        S+  + + I+S PF +   GYK+ L+  + GD  G  + +F+S  L + +G+F              D+ L WPF +++ I L++  D     +    S+K   C ++   LGRP  E+
Sbjct:  250 ADSNCEFKSLRSEMPRHLKESPG--IHLNLMAKTISIQKKQLELLSEIIEKQKE----QIFSLGSKVDSIEKISGSQIIWKIDHFNEKFNDSKSGKKQTIFSPPFLTSRHGYKLALSATLFGD--GKAKGKFMSLFLCICKGEF--------------DSLLSWPFSHKITITLLDQCDDIQERRHVSYSIKPNTCKENTPFLGRPTGER 437

[Close]

BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A1X7UYD4 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 GN=105312681 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 3.202e-7
Identity = 41/125 (32.80%), Postives = 62/125 (49.60%), Query Frame = 3

Query:  573 DKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARLGRPAREKYSDLL*I*FYRFIRH 947
            +KW YS PFY+ PGGYKM L+V   G+       R   G           +  Y HL+RG+ D  LEWPF+ E+ I L+N  + N++    +K+ + + D    R   +   +     F+RF+ H
Sbjct:  264 EKW-YSSPFYTSPGGYKMILSVCANGN-------RNAKGT---------HVSCYIHLVRGENDDYLEWPFQGEVTIELLNQLEDNNHKAYILKFNESTPDNSKNRVVEQ--GNGCGWGFHRFVSH 369

[Close]

BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A0M3K796 (Uncharacterized protein OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS16244 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 7.603e-7
Identity = 38/135 (28.15%), Postives = 67/135 (49.63%), Query Frame = 3

Query:  426 IEMIEFVIQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERD------KWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            ++M+   ++  ME     ++++Q K    E +    + W+ID + Q + +        I+S PF S   GYKM L+V + GD  G +R  ++S  +++ RG+F              DA LEWPF  ++ I L++
Sbjct:  167 LKMLLANMELSMETMGTNLETLQKKCATLEKLYGSQMIWRIDNVKQKQNEARSGTRSIIFSPPFASARHGYKMVLSVSLYGD--GQVRGEYMSAFISIMRGEF--------------DALLEWPFTNQIKITLLD 285

[Close]

BLAST of TNF receptor-associated factor vs. TrEMBL
Match: C3ZH16 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_85024 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.432e-6
Identity = 55/186 (29.57%), Postives = 84/186 (45.16%), Query Frame = 3

Query:  297 KISNPNNKLTTLRNRSGRVEQNPNGKLMHLN-KTLNSSRI*K*FIEMIEFVIQNEMEKCAYEIQSIQTKFGNAEFINF-GILDWQIDKLSQLERDKW------IYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGND 830
            +I N N  LT+   R     ++ NGKL  L  K  N S       E IE  +Q E+ +    I     K    E  ++ G L W+I+  SQ  ++        IYS PFY+   GYKMC+ V   G  GG  R   +S              +YFH+++   D+ L+WPF + +   L++ +D ++
Sbjct:  257 RIQNQNALLTSQHQRV----KSQNGKLESLTVKVCNQS-------EKIEL-LQTELLRKEAIIAEQMKKIKELEVTSYDGSLLWRINNFSQRHQEAASKQVPSIYSPPFYTSRFGYKMCIQVFANG--GGETRGTHMS--------------IYFHILKHRYDSVLQWPFPHNITFTLLDQSDDSN 414

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: ENSAMXT00000035169.1 (pep primary_assembly:Astyanax_mexicanus-2.0:9:3354744:3362219:1 gene:ENSAMXG00000029095.1 transcript:ENSAMXT00000035169.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 1.736e-6
Identity = 28/77 (36.36%), Postives = 43/77 (55.84%), Query Frame = 3

Query:  582 IYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:   28 LYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVVMRGEYDALLPWPFKQKVTLMLMD 88

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002130.1 (pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 gene:ENSPMAG00000001936.1 transcript:ENSPMAT00000002130.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 8.026e-8
Identity = 29/81 (35.80%), Postives = 43/81 (53.09%), Query Frame = 3

Query:  570 RDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            R   +YS+PFY+ P GYKMC    + GD               V RG    + ++F +MRG+ DA LEWPF+  + + L++
Sbjct:  288 RTLSLYSQPFYTSPFGYKMCARAYLNGD--------------GVGRGTH--LSLFFVVMRGEYDALLEWPFRRRVTLSLLD 352

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000074.1 (pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-1 gene:ENSPMAG00000000066.1 transcript:ENSPMAT00000000074.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 3.019e-7
Identity = 31/101 (30.69%), Postives = 51/101 (50.50%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G L W+I   ++ ++D        +YS+PFY+ P GY+MC    + GD               V RG    + ++F +MRG+ DA L WPF+  + + L++
Sbjct:  325 GKLVWKIRDYARRKQDAVSGRTLSLYSQPFYTSPFGYRMCARAYLNGD--------------GVGRGTH--LSLFFVVMRGEYDALLPWPFRQRVTLTLLD 409

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 46.9802 bits (110), Expect = 4.963e-6
Identity = 33/144 (22.92%), Postives = 64/144 (44.44%), Query Frame = 3

Query:  480 IQSIQTKFGNAEF-INFGILDWQIDKLSQL----------------ERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYD 860
            ++S++ +   +E  +  GI  W++++ S+L                +    ++S  FY+G  GY++CL + +             S LLA   G    + ++ HL++GD D  L WPFK  + I +++  + +      M   D
Sbjct:  376 VRSLEARVAESESRLCAGIFTWRVERFSRLLPGGLASGFASDGMHPDAPAVMHSSGFYTGVPGYRLCLRLQVLSGREVPAGAASPSVLLA---GRQPHLALFVHLLQGDYDHRLPWPFKGRIRISVVDQCERDSVHVTEMMDAD 516

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO29145 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4G8])

HSP 1 Score: 47.3654 bits (111), Expect = 3.068e-7
Identity = 32/109 (29.36%), Postives = 50/109 (45.87%), Query Frame = 3

Query:  528 GILDWQI-------DKLSQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDY 833
            GI  W+I       D+      +  I+S PFY+   GYK+ L   + G                  RG    + +Y  +M+GD DA LEWPFK ++  YLI+ ++  ++
Sbjct:    2 GIFLWKISNYKHQYDQAVASPEELAIFSPPFYTSQYGYKLRLKAYLNGRD----------------RGKGTHLSLYIIIMKGDYDALLEWPFKQKITFYLIDQSEKQNH 94

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO32849 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STV3])

HSP 1 Score: 49.2914 bits (116), Expect = 1.437e-6
Identity = 37/151 (24.50%), Postives = 71/151 (47.02%), Query Frame = 3

Query:  480 IQSIQTKFGNAEFINFGILDWQIDKLSQLERD------KWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQ---SMKYYDCSDDAR-LGRPAREK 902
            ++ + ++ G+ E I    + W+I+  ++  ++      + ++S  F +   GY++C +V + GD  G  +   +S  ++V +G F              DA L+WPF Y +  +L+N  +  +  K    S+K     D+   LGRP  EK
Sbjct:  290 MKQVDSRVGDLEKIAHSQIIWRIEDYTRKLKEAKAGNAETLFSPTFTTSKHGYRLCASVCLNGDGKG--KGTHMSVFVSVLKGAF--------------DALLKWPFHYRVTFFLLNQHENPEERKHVKFSIKPNPIPDNEPFLGRPRLEK 424

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO32851 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STV5])

HSP 1 Score: 49.2914 bits (116), Expect = 1.442e-6
Identity = 39/151 (25.83%), Postives = 68/151 (45.03%), Query Frame = 3

Query:  480 IQSIQTKFGNAEFINFGILDWQIDKLSQLERDK------WIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQ---SMKYYDCSDDAR-LGRPAREK 902
            I+  + +  + E I    L W+I+  ++  ++        ++S  F +   GY++C +V + GD  G  +   +S  ++V +G F              DA L+WPF Y +   L++  D     K    S+K   C+D+   LGRP  EK
Sbjct:  286 IEKHEKRLNDLEKIAHSQLIWRIEDYTRKMKEAKAGNTDTLFSPTFTTSKHGYRLCASVCLNGDGKG--KGTHMSVFISVLKGAF--------------DALLKWPFDYRVTFVLLDQNDDPAQRKHIRFSIKPNPCADNEPFLGRPRLEK 420

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO35237 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM27])

HSP 1 Score: 48.521 bits (114), Expect = 2.939e-6
Identity = 33/105 (31.43%), Postives = 51/105 (48.57%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERD------KWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADG 824
            G   W+IDK ++   +        IYS PFY+   GYK+C  +   GD  G+ R  ++S              ++  LMRG+ D+ L WPF+  + I LI+  +G
Sbjct:  380 GTFLWKIDKFTERYAEAKSGNATSIYSSPFYTHRFGYKLCARLFPNGD--GSARDNYLS--------------IFIALMRGEYDSILPWPFQETITITLIDQDNG 468

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO36181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJA2])

HSP 1 Score: 45.0542 bits (105), Expect = 3.850e-6
Identity = 33/105 (31.43%), Postives = 50/105 (47.62%), Query Frame = 3

Query:  540 WQIDKLS-QLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYV--YFHLMRGDCDAGLEWPFKYELNIYLINPADGND--YDK 839
            W+I   + Q E+ + I S  FY+   GYKM L   + G+                  GD  G ++  +  + +G+ DA LEWPFK ++ +YLI+  D     YDK
Sbjct:   19 WRIRNYNDQYEKKQTISSRLFYTSRYGYKMRLKSHLNGN------------------GDGKGTHLSLFVQITKGEYDALLEWPFKRKITLYLIDQIDQQRHMYDK 105

[Close]

BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf3 (TNF receptor associated factor 3 [Source:NCBI gene;Acc:101161601])

HSP 1 Score: 50.8322 bits (120), Expect = 1.239e-6
Identity = 33/101 (32.67%), Postives = 52/101 (51.49%), Query Frame = 3

Query:  528 GILDWQIDKLSQLERDKW------IYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLIN 812
            G L W+I    + ++D        +YS+PFY+G  GYKMC  V + GD           G+     G    + ++F +MRG+ DA L WPFK ++ + L++
Sbjct:  298 GTLIWKIRDYKRRKQDAVAGKTLSLYSQPFYTGYFGYKMCARVYLNGD-----------GM-----GKGTHLSLFFVVMRGEYDALLPWPFKQKVTLMLMD 382

[Close]

BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000000514.1 (SMESG000000514.1)

HSP 1 Score: 182.57 bits (462), Expect = 4.374e-92
Identity = 91/149 (61.07%), Postives = 103/149 (69.13%), Query Frame = 3

Query:  456 EMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARLGRPAREK 902
            EMEKC  EI+ IQTKFGNAEFINFGILDWQIDKLSQLER+KWIYSEPFYSGPGGYK+CL+VGI                      D GGIYV F L+ GDCDAGLEWPFKY + + +INPA+   Y  QS+K  D    A  GRPA ++
Sbjct:  294 EMEKCTNEIRQIQTKFGNAEFINFGILDWQIDKLSQLEREKWIYSEPFYSGPGGYKLCLSVGI--------------------SDDIGGIYVSFCLLHGDCDAGLEWPFKYSVTVNVINPANRKVYLSQSLKCPDSPAAAGWGRPASDR 422

HSP 2 Score: 160.229 bits (404), Expect = 4.374e-92
Identity = 88/122 (72.13%), Postives = 101/122 (82.79%), Query Frame = 2

Query:   35 ININKKKMESMTSEIDQLKQRMRLMEDKDRGKQGENERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSNQITKLKTENIQSKQQTDNIEKQIGTSRTKSERQIDAFKQNFE 400
            IN  + +MESMTS+ DQL QRM  ME+KDR  +GE ERLKGENDKL Q+LNA+KLRMCHASLK ND IQLCRIDNSLLS+++ KL  E  QSKQQ ++IEKQI TS+TKSERQIDAFKQN E
Sbjct:  160 INKFENQMESMTSKNDQLTQRMCRMEEKDRENRGEIERLKGENDKLNQQLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNAEISQSKQQIESIEKQIETSQTKSERQIDAFKQNCE 281

HSP 3 Score: 36.1946 bits (82), Expect = 4.374e-92
Identity = 23/38 (60.53%), Postives = 27/38 (71.05%), Query Frame = 1

Query:  880 WGG-QLERNIPIYCKYDFTDLSDIKNDKLLLKCRIEKL 990
            WG    +RN PIY   ++  LSDIKNDKLLLKC I+KL
Sbjct:  415 WGRPASDRNDPIY-YNNYIILSDIKNDKLLLKCGIKKL 451

[Close]

BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000029692.1 (SMESG000029692.1)

HSP 1 Score: 173.711 bits (439), Expect = 7.867e-92
Identity = 91/152 (59.87%), Postives = 102/152 (67.11%), Query Frame = 3

Query:  447 IQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARLGRPAREK 902
            IQNEMEKC  +I  IQTKFGNA FINFGILDWQIDKLSQLER+K IYSEPFYSGPGGYKMCLAV I GD                     G I V F LMRGDCDA L WPF+Y + I++INPA+GN Y +QS+K        R  RPA ++
Sbjct:  291 IQNEMEKCVNKIPPIQTKFGNA-FINFGILDWQIDKLSQLEREKLIYSEPFYSGPGGYKMCLAVDISGD---------------------GAILVRFCLMRGDCDAELVWPFRYSVTIHMINPANGNVYFRQSLKCPITFAAVRWVRPASDR 420

HSP 2 Score: 171.014 bits (432), Expect = 7.867e-92
Identity = 93/125 (74.40%), Postives = 106/125 (84.80%), Query Frame = 2

Query:   35 ININKKKMESMTSEIDQLKQRMRLMEDKDRGKQGENERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSNQITKLKTENIQSKQQTDNIEKQIGTSRTKSERQIDAFKQNFEQQQ 409
            IN  + +MESMTS+ DQL QRM LME+KDR  +GE ERLKGENDKL QKLNA+KLRMCHASLK ND IQLCRIDNSLLS+++ KL TE  QSK+Q ++IEKQI TS+TKSERQIDAFKQN EQQQ
Sbjct:  160 INKFENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLKGENDKLNQKLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQQ 284

HSP 3 Score: 33.8834 bits (76), Expect = 7.867e-92
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 1

Query:  925 DFTDLSDIKNDKLLLKCRIEK 987
            D+ +LSDIKNDKLLLKC I+K
Sbjct:  430 DYINLSDIKNDKLLLKCIIKK 450

[Close]

BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000029692.1 (SMESG000029692.1)

HSP 1 Score: 173.326 bits (438), Expect = 1.685e-91
Identity = 91/152 (59.87%), Postives = 102/152 (67.11%), Query Frame = 3

Query:  447 IQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARLGRPAREK 902
            IQNEMEKC  +I  IQTKFGNA FINFGILDWQIDKLSQLER+K IYSEPFYSGPGGYKMCLAV I GD                     G I V F LMRGDCDA L WPF+Y + I++INPA+GN Y +QS+K        R  RPA ++
Sbjct:  291 IQNEMEKCVNKIPPIQTKFGNA-FINFGILDWQIDKLSQLEREKLIYSEPFYSGPGGYKMCLAVDISGD---------------------GAILVRFCLMRGDCDAELVWPFRYSVTIHMINPANGNVYFRQSLKCPITFAAVRWVRPASDR 420

HSP 2 Score: 170.629 bits (431), Expect = 1.685e-91
Identity = 93/125 (74.40%), Postives = 106/125 (84.80%), Query Frame = 2

Query:   35 ININKKKMESMTSEIDQLKQRMRLMEDKDRGKQGENERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSNQITKLKTENIQSKQQTDNIEKQIGTSRTKSERQIDAFKQNFEQQQ 409
            IN  + +MESMTS+ DQL QRM LME+KDR  +GE ERLKGENDKL QKLNA+KLRMCHASLK ND IQLCRIDNSLLS+++ KL TE  QSK+Q ++IEKQI TS+TKSERQIDAFKQN EQQQ
Sbjct:  160 INKFENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLKGENDKLNQKLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQQ 284

HSP 3 Score: 33.4982 bits (75), Expect = 1.685e-91
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 1

Query:  925 DFTDLSDIKNDKLLLKCRIEK 987
            D+ +LSDIKNDKLLLKC I+K
Sbjct:  430 DYINLSDIKNDKLLLKCIIKK 450

[Close]

BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000000502.1 (SMESG000000502.1)

HSP 1 Score: 168.703 bits (426), Expect = 1.237e-90
Identity = 94/132 (71.21%), Postives = 109/132 (82.58%), Query Frame = 2

Query:   35 ININKKKMESMTSEIDQLKQRMRLMEDKDRGKQGENERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSNQITKLKTENIQSKQQTDNIEKQIGTSRTKSERQIDAFKQNFEQ---QQNIKV 421
            IN  + +MESMTS+ DQL QRM LME+KDR  +GE ERL GENDKL Q+LNA+KLRMCHASLK ND IQLCRIDNSLLS+++ KL TE  QSK+Q ++IEKQI TS+TKSERQIDAFKQN EQ   QQNIK+
Sbjct:  153 INKFENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLNGENDKLNQQLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQKLQQNIKI 284

HSP 2 Score: 158.303 bits (399), Expect = 1.237e-90
Identity = 83/152 (54.61%), Postives = 99/152 (65.13%), Query Frame = 3

Query:  447 IQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARLGRPAREK 902
            IQNEMEKCA EI+ IQTKFGNAEFINFG  +WQI KLSQ ER+K IYS+PF+SGP GYKMCL+V I+                     D G I VYFHLMRGD D  L+WPFKY + I  INP +GN Y   S++Y     D R  +P+R++
Sbjct:  284 IQNEMEKCANEIRLIQTKFGNAEFINFGFFEWQIYKLSQQERNKSIYSKPFFSGPIGYKMCLSVYIKC-------------------AD-GDICVYFHLMRGDSDEKLKWPFKYSVTIDAINPENGNVYRSHSVEYSKSPADDRWDKPSRDR 415

HSP 3 Score: 47.3654 bits (111), Expect = 1.237e-90
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 1

Query:  895 ERNIPIYCKYDFTDLSDIKNDKLLLKCRIEKL 990
            +RN PIY    FT+LSDIKNDKLLLKCRI+KL
Sbjct:  414 DRNAPIYYAGYFTNLSDIKNDKLLLKCRIKKL 445

[Close]

BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000000502.1 (SMESG000000502.1)

HSP 1 Score: 168.703 bits (426), Expect = 1.300e-90
Identity = 94/132 (71.21%), Postives = 109/132 (82.58%), Query Frame = 2

Query:   35 ININKKKMESMTSEIDQLKQRMRLMEDKDRGKQGENERLKGENDKLIQKLNAIKLRMCHASLKGNDIIQLCRIDNSLLSNQITKLKTENIQSKQQTDNIEKQIGTSRTKSERQIDAFKQNFEQ---QQNIKV 421
            IN  + +MESMTS+ DQL QRM LME+KDR  +GE ERL GENDKL Q+LNA+KLRMCHASLK ND IQLCRIDNSLLS+++ KL TE  QSK+Q ++IEKQI TS+TKSERQIDAFKQN EQ   QQNIK+
Sbjct:  160 INKFENQMESMTSKNDQLTQRMCLMEEKDRENRGEIERLNGENDKLNQQLNAMKLRMCHASLKANDNIQLCRIDNSLLSDEMAKLNTEISQSKRQIESIEKQIETSQTKSERQIDAFKQNCEQQKLQQNIKI 291

HSP 2 Score: 158.303 bits (399), Expect = 1.300e-90
Identity = 83/152 (54.61%), Postives = 99/152 (65.13%), Query Frame = 3

Query:  447 IQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERDKWIYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYVYFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARLGRPAREK 902
            IQNEMEKCA EI+ IQTKFGNAEFINFG  +WQI KLSQ ER+K IYS+PF+SGP GYKMCL+V I+                     D G I VYFHLMRGD D  L+WPFKY + I  INP +GN Y   S++Y     D R  +P+R++
Sbjct:  291 IQNEMEKCANEIRLIQTKFGNAEFINFGFFEWQIYKLSQQERNKSIYSKPFFSGPIGYKMCLSVYIKC-------------------AD-GDICVYFHLMRGDSDEKLKWPFKYSVTIDAINPENGNVYRSHSVEYSKSPADDRWDKPSRDR 422

HSP 3 Score: 47.3654 bits (111), Expect = 1.300e-90
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 1

Query:  895 ERNIPIYCKYDFTDLSDIKNDKLLLKCRIEKL 990
            +RN PIY    FT+LSDIKNDKLLLKCRI+KL
Sbjct:  421 DRNAPIYYAGYFTNLSDIKNDKLLLKCRIKKL 452

The following BLAST results are available for this feature:

BLAST of TNF receptor-associated factor vs. Ensembl Human
BLAST of TNF receptor-associated factor vs. Ensembl Celegans
BLAST of TNF receptor-associated factor vs. Ensembl Fly
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
BLAST of TNF receptor-associated factor vs. TrEMBL
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
BLAST of TNF receptor-associated factor vs. Ensembl Yeast
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
BLAST of TNF receptor-associated factor vs. Planmine SMEST

BLAST of TNF receptor-associated factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TRAF3	6.220e-6	31.68	TNF receptor associated factor 3 [Source:HGNC Symb...	[more]
TRAF3	6.719e-6	31.68	TNF receptor associated factor 3 [Source:HGNC Symb...	[more]
TRAF3	6.719e-6	31.68	TNF receptor associated factor 3 [Source:HGNC Symb...	[more]
TRAF3	7.190e-6	31.68	TNF receptor associated factor 3 [Source:HGNC Symb...	[more]
TRAF3	7.190e-6	31.68	TNF receptor associated factor 3 [Source:HGNC Symb...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of TNF receptor-associated factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
traf3	3.895e-6	31.68	TNF receptor-associated factor 3 [Source:ZFIN;Acc:...	[more]
BX901915.1	4.740e-6	29.70	pep chromosome:GRCz11:CHR_ALT_CTG5_1_18:28764891:2...	[more]
AL844899.1	4.740e-6	29.70	pep chromosome:GRCz11:CHR_ALT_CTG5_2_4:28756846:28...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hspb6	4.564e-6	30.28	heat shock protein family B (small) member 6 [Sour...	[more]
TRAF2	5.012e-6	26.27	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
TRAF2	5.230e-6	26.27	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
ssh2	6.005e-6	31.68	slingshot protein phosphatase 2 [Source:Xenbase;Ac...	[more]
ssh2	6.365e-6	31.68	slingshot protein phosphatase 2 [Source:Xenbase;Ac...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Traf3	5.167e-6	31.68	TNF receptor-associated factor 3 [Source:MGI Symbo...	[more]
Traf3	5.385e-6	31.68	TNF receptor-associated factor 3 [Source:MGI Symbo...	[more]
Traf3	5.385e-6	31.68	TNF receptor-associated factor 3 [Source:MGI Symbo...	[more]
Traf5	5.933e-6	24.07	TNF receptor-associated factor 5 [Source:MGI Symbo...	[more]
Traf5	5.933e-6	24.07	TNF receptor-associated factor 5 [Source:MGI Symbo...	[more]

BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of TNF receptor-associated factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1X7U807	1.357e-7	32.71	Uncharacterized protein OS=Amphimedon queenslandic...	[more]
A0A3M7SHF5	2.661e-7	25.71	TNF receptor-associated factor 4-like OS=Brachionu...	[more]
A0A1X7UYD4	3.202e-7	32.80	Uncharacterized protein OS=Amphimedon queenslandic...	[more]
A0A0M3K796	7.603e-7	28.15	Uncharacterized protein OS=Anisakis simplex OX=626...	[more]
C3ZH16	1.432e-6	29.57	Uncharacterized protein OS=Branchiostoma floridae ...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSAMXT00000035169.1	1.736e-6	36.36	pep primary_assembly:Astyanax_mexicanus-2.0:9:3354...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSPMAT00000002130.1	8.026e-8	35.80	pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 g...	[more]
ENSPMAT00000000074.1	3.019e-7	30.69	pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-...	[more]
traf6	4.963e-6	22.92	TNF receptor-associated factor 6 [Source:NCBI gene...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO29145	3.068e-7	29.36	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32849	1.437e-6	24.50	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32851	1.442e-6	25.83	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35237	2.939e-6	31.43	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO36181	3.850e-6	31.43	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
traf3	1.239e-6	32.67	TNF receptor associated factor 3 [Source:NCBI gene...	[more]

BLAST of TNF receptor-associated factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000000514.1	4.374e-92	61.07	SMESG000000514.1	[more]
SMESG000029692.1	7.867e-92	59.87	SMESG000029692.1	[more]
SMESG000029692.1	1.685e-91	59.87	SMESG000029692.1	[more]
SMESG000000502.1	1.237e-90	71.21	SMESG000000502.1	[more]
SMESG000000502.1	1.300e-90	71.21	SMESG000000502.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence

transcript sequence

>SMED30007937 ID=SMED30007937|Name=TNF receptor-associated factor|organism=Schmidtea mediterranea sexual|type=transcript|length=1123bp
ATAATCTTTCATATTTGGATAATTTACAAAAGATATAAATATAAATAAAA
AAAAGATGGAATCAATGACGAGTGAAATTGATCAATTGAAGCAGAGAATG
CGCCTGATGGAAGACAAAGATCGGGGAAAACAGGGAGAAAATGAGAGATT
GAAGGGAGAAAACGATAAACTCATTCAGAAATTAAATGCAATAAAGCTGA
GAATGTGCCACGCGTCACTGAAAGGGAATGACATCATCCAACTTTGCCGC
ATTGATAATTCTTTGTTGAGTAATCAAATTACCAAATTAAAGACGGAAAA
TATCCAATCCAAACAACAAACTGACAACATTGAGAAACAGATCGGGACGA
GTCGAACAAAATCCGAACGGCAAATTGATGCATTTAAACAAAACTTTGAA
CAGCAGCAGAATATAAAAGTGATTTATAGAAATGATTGAATTTGTGATTC
AGAATGAAATGGAGAAATGCGCCTATGAAATCCAGTCGATTCAAACAAAA
TTTGGAAATGCAGAATTTATAAATTTCGGAATTTTGGATTGGCAAATCGA
TAAATTATCGCAACTGGAGAGAGATAAATGGATTTACAGCGAGCCATTTT
ATTCCGGGCCGGGCGGATACAAAATGTGCTTGGCGGTGGGTATTAGGGGT
GATTTTGGTGGTGCAATTCGTGTTCGTTTCATTAGTGGTTTATTAGCGGT
GTTTAGGGGTGATTTTGGTGGAATTTATGTTTATTTCCATTTGATGCGTG
GCGATTGCGATGCCGGATTGGAATGGCCATTCAAATATGAACTGAACATA
TATTTGATAAACCCGGCAGATGGAAATGATTATGATAAACAATCGATGAA
ATATTATGACTGCTCTGATGATGCCAGATTGGGGAGGCCAGCTAGAGAGA
AATATTCCGATTTATTGTAAATATGATTTTACCGATTTATCAGACATTAA
AAACGACAAATTGCTGCTCAAATGCAGAATAGAAAAATTGTGAAAAACTT
TATCAAATATTTCAATAAAAATTTTGATTTATTTTCTCACTTTTCCTCAG
CTCAGCTGATAAATTTATGCAAATTGTCGGTTTTGATAGGCGCGCGATAT
CGGCCAATCAAATCACTGATCAG

protein sequence of SMED30007937-orf-1

>SMED30007937-orf-1 ID=SMED30007937-orf-1|Name=SMED30007937-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
MESMTSEIDQLKQRMRLMEDKDRGKQGENERLKGENDKLIQKLNAIKLRM
CHASLKGNDIIQLCRIDNSLLSNQITKLKTENIQSKQQTDNIEKQIGTSR
TKSERQIDAFKQNFEQQQNIKVIYRND*

protein sequence of SMED30007937-orf-2

>SMED30007937-orf-2 ID=SMED30007937-orf-2|Name=SMED30007937-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MIEFVIQNEMEKCAYEIQSIQTKFGNAEFINFGILDWQIDKLSQLERDKW
IYSEPFYSGPGGYKMCLAVGIRGDFGGAIRVRFISGLLAVFRGDFGGIYV
YFHLMRGDCDAGLEWPFKYELNIYLINPADGNDYDKQSMKYYDCSDDARL
GRPAREKYSDLL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0001817	regulation of cytokine production
GO:0006470	protein dephosphorylation
GO:0008063	Toll signaling pathway
GO:0032088	negative regulation of NF-kappaB transcription factor activity
GO:0033209	tumor necrosis factor-mediated signaling pathway
GO:0042981	regulation of apoptotic process
GO:0045087	innate immune response
GO:0050688	regulation of defense response to virus
GO:0006955	immune response
GO:0007165	signal transduction
GO:0016567	protein ubiquitination

Vocabulary: **molecular function**
Term	Definition
GO:0005164	tumor necrosis factor receptor binding
GO:0008270	zinc ion binding
GO:0046872	metal ion binding
GO:0005515	protein binding
GO:0004842	ubiquitin-protein transferase activity

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000074	oocyte
PLANA:0000231	vitelline gland
PLANA:0002032	epidermal cell
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR008974	TRAF-like

Main menu

TNF receptor-associated factor

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Owlery

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