C2H2-type domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007927 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A183ADN5 (C2H2-type domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS5070 PE=4 SV=1) HSP 1 Score: 166.007 bits (419), Expect = 1.716e-38 Identity = 74/116 (63.79%), Postives = 92/116 (79.31%), Query Frame = 3 Query: 627 SGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRR 974 + +NG+ + W + KV VG+D+TRKGERSLV+DG+KFTKSRDG +RVFWRCSRRECKATAVTV DRVEH+RA+H H+PP+AAEFF + RC+ QEVRFN +A+ R R RR Sbjct: 89 TAKNGE--KKWNLVKVHVGFDVTRKGERSLVIDGYKFTKSRDGMGDRVFWRCSRRECKATAVTVSDRVEHIRAVHTHQPPVAAEFFAEN---TVRCE-QEVRFNNTASYPRSRVRR 198
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A0X3Q5R0 (C2H2-type domain-containing protein OS=Schistocephalus solidus OX=70667 GN=TR139982 PE=4 SV=1) HSP 1 Score: 162.54 bits (410), Expect = 3.055e-38 Identity = 69/106 (65.09%), Postives = 85/106 (80.19%), Query Frame = 3 Query: 657 WTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRR 974 W + KV+VG+D+TRKGERSLV+DG+KFTKSRDG ++RVFWRCSRRECKATAVTV DRVEHVR +H H+PP+A EFF G +LQ VRFN + ++ +PR RR Sbjct: 127 WNLMKVRVGFDVTRKGERSLVIDGYKFTKSRDGMSDRVFWRCSRRECKATAVTVSDRVEHVRTLHTHKPPLAGEFFVESAG---GAKLQTVRFNYAPSSTKPRARR 229
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A504YTF3 (Zinc finger FLYWCH type OS=Fasciola gigantica OX=46835 GN=FGIG_11096 PE=4 SV=1) HSP 1 Score: 164.466 bits (415), Expect = 6.206e-38 Identity = 73/114 (64.04%), Postives = 89/114 (78.07%), Query Frame = 3 Query: 633 RNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRR 974 +N D + W + KV+VG+D+TRKGERSLV+DG+KFTKSRDG +RVFWRCSRRECKATAVTV D+VEH+RA+H+H+PP+AAEFF S+ QEVRFN SA R R RR Sbjct: 88 KNAD--KKWNLVKVRVGFDVTRKGERSLVIDGYKFTKSRDGIGDRVFWRCSRRECKATAVTVADKVEHIRAMHSHQPPVAAEFFADNPARSE----QEVRFNNSATYSRSRVRR 195
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A4E0RDP2 (Zinc finger FLYWCH type OS=Fasciola hepatica OX=6192 GN=D915_004690 PE=4 SV=1) HSP 1 Score: 163.31 bits (412), Expect = 1.764e-37 Identity = 72/114 (63.16%), Postives = 89/114 (78.07%), Query Frame = 3 Query: 633 RNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRR 974 +N D + W + KV+VG+D+TRKGERSLV+DG+KFTKSRDG +RVFWRCSRRECKATAVTV D+VEH+RA+H+H+PP+AAEFF S+ QEVRFN +A R R RR Sbjct: 88 KNAD--KKWNLVKVRVGFDVTRKGERSLVIDGYKFTKSRDGIGDRVFWRCSRRECKATAVTVADKVEHIRAMHSHQPPVAAEFFADNPARSE----QEVRFNNAATYSRSRVRR 195
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A183TAY0 (C2H2-type domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS13628 PE=4 SV=1) HSP 1 Score: 161.77 bits (408), Expect = 6.770e-37 Identity = 69/106 (65.09%), Postives = 86/106 (81.13%), Query Frame = 3 Query: 657 WTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRR 974 W + KV+VG+D+TRKGERSLV+DG+KFTKSRDG ++RVFWRCSRRECKATAVTV DRVEHVR +H H+PP+A EFF G + +LQ VRFN + ++ +PR RR Sbjct: 182 WNLMKVRVGFDVTRKGERSLVIDGYKFTKSRDGMSDRVFWRCSRRECKATAVTVSDRVEHVRTLHTHKPPLAGEFFVESAGGA---KLQTVRFNYAPSSTKPRARR 284
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000032739.1 (SMESG000032739.1) HSP 1 Score: 955.666 bits (2469), Expect = 0.000e+0 Identity = 492/495 (99.39%), Postives = 492/495 (99.39%), Query Frame = 3 Query: 474 MSEVNYTSMESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSSIKSGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRRSTVXXXXXXXXXXXXXVKDISNSNGIKIEKKPANQNVTNPKPLISLVSAQPVFQIHSADKKHSYSSCSGLQKTPSVKEQVRDFILRNSNIQPQSQTENNLSVAQFLISPASQTQFIQTNTTPLNSANSATGSTIFNFSTMQINASPNTGSDSTSEAPSHKSTPGGLMNLPTTPHDETQYKSKTNSDCSGVXXXXXXXXXXXXXXKNGPNRHRSSSDSEWMAGNSSSTVSYEHESHELSPCAMPAPPLSADEQSYVATGVVHQSAPTPTRLIHWKKEKLRESESSDELFRRERKRRHEDINSNNCNTMIGLGASCLPELHAPSGVSCAG 1958 MSEVNYTSMESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSSIKSGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRRSTVSGSEGGNAEESSSVKDISNSNGIKIEKKPANQNVTNPKPLISLVSAQPVFQIHSADKKHSYSSCSGLQKTPSVKEQVRDFILRNSNIQPQSQTENNLSVAQFLISPASQTQFIQTN TPLNSANSATGSTIFNFSTMQINASPNTGSDSTSEAPSHKSTPGGLMNLPTTPHDETQYKSKTNSDCSGVNQLNLEQIIQILLNKNGPNRHRSSSDSEWMAGNSSSTVSYEHESHELSPCAMPAPPLSADEQSYVATGVVHQSAPTPTRLIHWKKEKLRESESSDELFRRERKRRHEDINSNNCNTMIGLGASCLPEL AP GVSCAG Sbjct: 1 MSEVNYTSMESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSSIKSGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRRSTVSGSEGGNAEESSSVKDISNSNGIKIEKKPANQNVTNPKPLISLVSAQPVFQIHSADKKHSYSSCSGLQKTPSVKEQVRDFILRNSNIQPQSQTENNLSVAQFLISPASQTQFIQTNPTPLNSANSATGSTIFNFSTMQINASPNTGSDSTSEAPSHKSTPGGLMNLPTTPHDETQYKSKTNSDCSGVNQLNLEQIIQILLNKNGPNRHRSSSDSEWMAGNSSSTVSYEHESHELSPCAMPAPPLSADEQSYVATGVVHQSAPTPTRLIHWKKEKLRESESSDELFRRERKRRHEDINSNNCNTMIGLGASCLPELRAPPGVSCAG 495 HSP 2 Score: 115.546 bits (288), Expect = 1.376e-26 Identity = 59/80 (73.75%), Postives = 61/80 (76.25%), Query Frame = 2 Query: 1907 CVMLAGIACSVWSEL--------CWRFGAGIYSCNGCGSIKRAVLIPQFIPPDNHSQTDGSHAKAVIRIVSRGSRRPGSV 2122 C + G+ S EL FGAGIYSCNGCGSIKRAVLIPQFIPPDNHSQTDGSHAKAVIRIVSRGSRRPGSV Sbjct: 471 CNTMIGLGASCLPELRAPPGVSCAGGFGAGIYSCNGCGSIKRAVLIPQFIPPDNHSQTDGSHAKAVIRIVSRGSRRPGSV 550
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000032739.1 (SMESG000032739.1) HSP 1 Score: 939.102 bits (2426), Expect = 0.000e+0 Identity = 484/487 (99.38%), Postives = 484/487 (99.38%), Query Frame = 3 Query: 498 MESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSSIKSGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRRSTVXXXXXXXXXXXXXVKDISNSNGIKIEKKPANQNVTNPKPLISLVSAQPVFQIHSADKKHSYSSCSGLQKTPSVKEQVRDFILRNSNIQPQSQTENNLSVAQFLISPASQTQFIQTNTTPLNSANSATGSTIFNFSTMQINASPNTGSDSTSEAPSHKSTPGGLMNLPTTPHDETQYKSKTNSDCSGVXXXXXXXXXXXXXXKNGPNRHRSSSDSEWMAGNSSSTVSYEHESHELSPCAMPAPPLSADEQSYVATGVVHQSAPTPTRLIHWKKEKLRESESSDELFRRERKRRHEDINSNNCNTMIGLGASCLPELHAPSGVSCAG 1958 MESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSSIKSGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRRSTVSGSEGGNAEESSSVKDISNSNGIKIEKKPANQNVTNPKPLISLVSAQPVFQIHSADKKHSYSSCSGLQKTPSVKEQVRDFILRNSNIQPQSQTENNLSVAQFLISPASQTQFIQTN TPLNSANSATGSTIFNFSTMQINASPNTGSDSTSEAPSHKSTPGGLMNLPTTPHDETQYKSKTNSDCSGVNQLNLEQIIQILLNKNGPNRHRSSSDSEWMAGNSSSTVSYEHESHELSPCAMPAPPLSADEQSYVATGVVHQSAPTPTRLIHWKKEKLRESESSDELFRRERKRRHEDINSNNCNTMIGLGASCLPEL AP GVSCAG Sbjct: 1 MESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSSIKSGRNGDCSRSWTMEKVQVGYDITRKGERSLVVDGFKFTKSRDGTNNRVFWRCSRRECKATAVTVCDRVEHVRAIHAHEPPMAAEFFGSQEGVSQRCQLQEVRFNTSAANMRPRYRRSTVSGSEGGNAEESSSVKDISNSNGIKIEKKPANQNVTNPKPLISLVSAQPVFQIHSADKKHSYSSCSGLQKTPSVKEQVRDFILRNSNIQPQSQTENNLSVAQFLISPASQTQFIQTNPTPLNSANSATGSTIFNFSTMQINASPNTGSDSTSEAPSHKSTPGGLMNLPTTPHDETQYKSKTNSDCSGVNQLNLEQIIQILLNKNGPNRHRSSSDSEWMAGNSSSTVSYEHESHELSPCAMPAPPLSADEQSYVATGVVHQSAPTPTRLIHWKKEKLRESESSDELFRRERKRRHEDINSNNCNTMIGLGASCLPELRAPPGVSCAG 487 HSP 2 Score: 115.546 bits (288), Expect = 1.331e-26 Identity = 59/80 (73.75%), Postives = 61/80 (76.25%), Query Frame = 2 Query: 1907 CVMLAGIACSVWSEL--------CWRFGAGIYSCNGCGSIKRAVLIPQFIPPDNHSQTDGSHAKAVIRIVSRGSRRPGSV 2122 C + G+ S EL FGAGIYSCNGCGSIKRAVLIPQFIPPDNHSQTDGSHAKAVIRIVSRGSRRPGSV Sbjct: 463 CNTMIGLGASCLPELRAPPGVSCAGGFGAGIYSCNGCGSIKRAVLIPQFIPPDNHSQTDGSHAKAVIRIVSRGSRRPGSV 542 The following BLAST results are available for this feature:
BLAST of C2H2-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of C2H2-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007927 ID=SMED30007927|Name=C2H2-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2609bpback to top protein sequence of SMED30007927-orf-1 >SMED30007927-orf-1 ID=SMED30007927-orf-1|Name=SMED30007927-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=575bp MFMSEVNYTSMESSPQEEISNSDVENVAMSPDNNRIDVVSVEQLPFDDQSback to top Annotated Terms
The following terms have been associated with this transcript:
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