SMED30007922

Overview
NameSMED30007922
Smed IDSMED30007922
Length (bp)2187
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007922 (SMED30007922) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007922 (SMED30007922) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007922 (SMED30007922) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007922 (SMED30007922) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007922

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30007922SMESG000070344.1 SmedASXL_013378SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30007922SMESG000070344.1 SmedASXL_013378SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30007922 vs. Planmine SMEST
Match: SMESG000070344.1 (SMESG000070344.1)

HSP 1 Score: 1302.73 bits (3370), Expect = 0.000e+0
Identity = 637/638 (99.84%), Postives = 637/638 (99.84%), Query Frame = 3 
Query:   63 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPETKKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKNILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK 1976
            MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPETKKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATK ILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK
Sbjct:    1 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPETKKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKTILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK 638
[Close]
BLAST of SMED30007922 vs. Planmine SMEST
Match: SMESG000070604.1 (SMESG000070604.1)

HSP 1 Score: 973.385 bits (2515), Expect = 0.000e+0
Identity = 465/642 (72.43%), Postives = 548/642 (85.36%), Query Frame = 3 
Query:   63 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPET----KKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKNILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK 1976
            MNTDL+YVSNIPS+MKPVLLLHELENKWSRMLYLENSFSG+RDA IYNP  S+  F+ GVQ+LR KVF+D+K LSVSYVWFPPPNVFITTMIPDGAS+LSY+FVEN  KE NVAELTA LP QQHDIK+WDIPERQDKCLVIKLPLSS+T+MRSYVLGTLRNRK+KGYFP VYE+IIS+T ELSKVMVPKT   TESSES MANFLFL+NIPSKLNVKSLVDSTK DNIKGCRVFF+ET+NK K+GF YIPKTLPYNKF+ LFEKL++ EY  DDGT+ +IDL+EVWFQP +A+PP LT++E  S  PPEF+L  YLYLHNIPPN+VASQLLMG+P  VKLYVF+VNDLSTQIGFVVLRGL+PE CQ++I+HFR+LV+GGLPILVDEIYLKCY+L DI +RD E+IPR     P++     KR ++NS NG +ES +R IS IKLSK P EGW LMKS+FICL+NLPN+++RSAI+F+IG  VGGCVL + S+GKP+AFVYLPDCE  LWE ++SKLA+KNI GN++DIHE+YLQS+D+ M    NE++ S FIFIYN P TPVEDE SL Y+++I+DI FMDTD G RC +A LY+GT+E Q+ DCLN+IR+IK+  KPL+ E  W A+V VDV QPP+KK
Sbjct:    1 MNTDLLYVSNIPSTMKPVLLLHELENKWSRMLYLENSFSGVRDALIYNPLSSIQIFYNGVQRLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENFHKEGNVAELTADLPLQQHDIKIWDIPERQDKCLVIKLPLSSSTAMRSYVLGTLRNRKVKGYFPRVYEMIISNTIELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPR-----PQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKK 637
[Close]
BLAST of SMED30007922 vs. Planmine SMEST
Match: SMESG000070322.1 (SMESG000070322.1)

HSP 1 Score: 971.844 bits (2511), Expect = 0.000e+0
Identity = 464/642 (72.27%), Postives = 547/642 (85.20%), Query Frame = 3 
Query:   63 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPET----KKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKNILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK 1976
            MNTDL+YVSNIPS+MKPVLLLHELENKWSRMLYLENSFSG+RDA IYNP  S+  F+ GVQ+LR KVF+D+K LSVSYVWFPPPNVFITTMIPDGAS+LSY+FVEN  KE NVAELTA LP QQHDIK+WDIPERQDKCLVIKLPLSS+T+MRSYVLGTLRNRK+KGYFP VYE+IIS+T ELSKVMVPKT   TESSES MANFLFL+NIPSKLNVKSLVDSTK DNIKGCRVFF+ET+NK K+GF YIPKTLPYNKF+ LFEKL++ EY  DDGT+ +IDL+EVWFQP +A+PP LT++E  S  PPEF+L  YLYLHNIPPN+VASQLLMG+P  VKLYVF+VNDLSTQIGFVVLRGL+PE CQ++I+HFR+LV+GGLPILVDEIYLKCY+L DI +RD E+IPR     P++     KR ++NS NG +ES +R IS IKLSK P EGW LMKS+FICL+NLPN+++RSAI+F+IG  VGGCVL + S+GKP+AFVYLPDCE  LWE ++ KLA+KNI GN++DIHE+YLQS+D+ M    NE++ S FIFIYN P TPVEDE SL Y+++I+DI FMDTD G RC +A LY+GT+E Q+ DCLN+IR+IK+  KPL+ E  W A+V VDV QPP+KK
Sbjct:    1 MNTDLLYVSNIPSTMKPVLLLHELENKWSRMLYLENSFSGVRDALIYNPLSSIQIFYNGVQRLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENFHKEGNVAELTADLPLQQHDIKIWDIPERQDKCLVIKLPLSSSTAMRSYVLGTLRNRKVKGYFPRVYEMIISNTIELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPR-----PQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKK 637
[Close]
BLAST of SMED30007922 vs. Planmine SMEST
Match: SMESG000017548.1 (SMESG000017548.1)

HSP 1 Score: 909.442 bits (2349), Expect = 0.000e+0
Identity = 444/640 (69.38%), Postives = 525/640 (82.03%), Query Frame = 3 
Query:   63 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPETKKRCVENSINGDIE--SNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKNILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK 1976
            MNTDL+YVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDA IYNP+ S+  F+ GVQKLR KVF+D+K LSVSYVWFPPPNVFITTMIPDGAS+LSY+FVENL KE NVAELTA LP QQHDIKLWDIPERQDKCL+IKLP SSNT+MRSYVLGTLRNRK+KG+FP VYE+IISDTPELSKVMVPKT   TESSES MANFLFL+NIPSKLNVKSLVDSTK DNIKGCRVFF+ET+NK K+GF YIPKTLPYNKF+ LFEKL++ EY  DDGT+ +IDL+EVWFQP +A+PP LT++E  S  PPEF+L  YLYLHNIPPN+VASQLLMG+P  VKLYVF+VNDLSTQIGFVVLRGL+PE CQ++I+HFR+LV+GGLPILVDEIYLKCY+L DI +RD E+IPR     P   +  +  ++   +   SN+    ++KLS P  +GW LMKS+FICL+NLPN+++RSAI+F+IG  VGGCVL + S+GKP+AFVYLPDCE  LWE ++ KLA+K     +  I  ++ +S+   M         S FIFIYN P TPVEDE SL Y+++I+DI FMDTD G RC +A LY+GT+E Q+ DCLN+IR+IK+  KPL+ E  W A+V VDV QPP+KK
Sbjct:    1 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPR-----PHPLESIIREALTIQLMALSNQLKDESLKLSCP--KGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASK----TFKAISWIFTRSTCSRMTIAWQLLAMSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKK 629
[Close]
BLAST of SMED30007922 vs. Planmine SMEST
Match: SMESG000017548.1 (SMESG000017548.1)

HSP 1 Score: 841.262 bits (2172), Expect = 0.000e+0
Identity = 419/640 (65.47%), Postives = 500/640 (78.12%), Query Frame = 3 
Query:   63 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPETKKRCVENSINGDIE--SNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKNILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKK 1976
            MNTDL+YVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDA IYNP+ S+  F+ GVQKLR KVF+D+K LSVSYVWFPPPNVFITTMIPDGAS+LSY+FVENL KE NVAELTA LP QQHDIKLWDIPERQDKCL+IKLP SSNT+MRSYVLGTLRNRK+KG+FP VYE+IISDTPELSKVMVPKT   TESSES MANFLFL+NIPSKLNVKSLVDSTK DNIKGCRVFF+ET+NK K+GF YIPKTLPYNKF+ LFEKL++ EY  DDGT+ +IDL+EVWFQP +A+PP LT++E  S  PPEF+L  YLYLHNIPPN+VASQLLMG+P  VKLYVF+VNDLSTQIGFVVLRGL+PE CQ++I+HFR+LV+GGLPILVDEIYLKCY+L DI +RD E+IPR     P   +  +  ++   +   SN+    ++KLS P  +GW LMKS+FICL+NLPN+++RSAI+F+IG  VGGCVL + S+GKP+AFVYLPDCE  LWE ++ KLA+K     +  I  ++ +S+   M                                 + + ++ M      RC +A LY+GT+E Q+ DCLN+IR+IK+  KPL+ E  W A+V VDV QPP+KK
Sbjct:    1 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPR-----PHPLESIIREALTIQLMALSNQLKDESLKLSCP--KGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASK----TFKAISWIFTRSTCSRM--------------------------------TIAWQLLAM------RCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKK 591
The following BLAST results are available for this feature:
BLAST of SMED30007922 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007922 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070344.10.000e+099.84SMESG000070344.1[more]
SMESG000070604.10.000e+072.43SMESG000070604.1[more]
SMESG000070322.10.000e+072.27SMESG000070322.1[more]
SMESG000017548.10.000e+069.38SMESG000017548.1[more]
SMESG000017548.10.000e+065.47SMESG000017548.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007922 ID=SMED30007922|Name=SMED30007922|organism=Schmidtea mediterranea sexual|type=transcript|length=2187bp
TTTTAATTGTGTTAATTTGGCCATTCATTTTAGTTCTGTACTAATAATAA
TTTTAAACTATTATGAATACTGATTTAATTTATGTATCGAATATTCCAAG
CTCAATGAAGCCAGTTCTGTTATTACACGAATTAGAAAATAAGTGGTCGC
GAATGCTTTATTTGGAAAATTCATTTAGCGGAATGCGAGATGCATTTATA
TATAATCCAACTCCATCTTTACCTCATTTTCATAAAGGTGTTCAAAAACT
ACGAGAAAAAGTTTTCGAAGATTCCAAATACCTTAGTGTTTCGTACGTTT
GGTTTCCTCCTCCCAATGTTTTTATAACAACAATGATTCCTGATGGAGCA
TCTATGCTTTCGTATTTATTTGTGGAGAATTTACCCAAAGAAAGTAACGT
GGCTGAATTGACAGCTGGTTTACCATTTCAACAGCATGATATTAAATTAT
GGGATATTCCAGAGCGACAGGACAAATGTTTGGTTATTAAATTGCCTTTA
TCAAGTAATACTTCAATGAGAAGTTACGTGTTAGGAACATTACGTAATCG
AAAGCTTAAAGGGTACTTTCCTCACGTTTATGAAATAATTATTTCCGACA
CGCCGGAGTTATCTAAAGTTATGGTTCCAAAAACTTTTTTACCCACAGAA
TCATCTGAATCATATATGGCAAATTTTTTGTTTCTTTACAACATTCCTTC
GAAGTTAAACGTGAAAAGCTTGGTAGACTCTACCAAATTCGATAATATTA
AAGGCTGTCGAGTTTTCTTTATTGAAACCATGAACAAATCAAAATTAGGA
TTTTCATATATTCCAAAAACATTGCCTTATAACAAATTTGTCAGTTTGTT
TGAGAAGTTGAAAAGCACTGAATATCCTTTAGACGATGGTACTGCTCGTA
AAATTGATTTGAATGAAGTTTGGTTTCAACCAATCAATGCCGAGCCTCCT
AAATTGACAGAAGATGAAGTGCTGTCAGAGAATCCACCGGAATTTATACT
AACACAATATTTATATTTACATAATATTCCTCCAAATTATGTAGCATCAC
AGTTATTAATGGGAGCGCCATGTATTGTCAAATTGTATGTTTTCTCAGTG
AACGACTTATCGACTCAAATCGGATTTGTTGTGCTTCGAGGACTTACTCC
TGAATGTTGTCAAATAATAATCGATCATTTTCGTAGTCTTGTGCTAGGTG
GTTTACCAATACTGGTTGATGAAATTTATCTGAAATGCTATGATTTACCC
GATATCTCATCCCGTGACACAGAGCACATACCTCGTCTAGTTCTAAATCT
TCCCGAAACCAAGAAACGCTGCGTGGAAAACTCAATTAACGGAGATATCG
AATCAAATAGAAGACTTATATCGAACATTAAATTATCCAAACCTCCTTCA
GAAGGCTGGGCTTTAATGAAGTCGGACTTTATCTGTCTTACTAACCTACC
GAATGCACTTAGTCGATCTGCAATCATTTTCGATATCGGCGCTGTTGTCG
GCGGGTGTGTATTAGCTTCTTCTTCTGAAGGCAAACCATTTGCGTTTGTC
TACCTTCCAGACTGTGAGGAATTGCTTTGGGAAAAAATTGTCAGTAAATT
AGCTACAAAAAACATACTGGGTAACTATTTGGACATCCATGAAGTTTACC
TGCAATCAAGTGACGAATCTATGCCGAGTGGTAACAACGAATTTCAATCT
AGTTATTTCATATTCATATATAATCTTCCAATGACGCCCGTTGAAGATGA
AAACTCCCTTTTTTATAACGATGTTATTTATGATATAGTTTTCATGGATA
CGGATACTGGAAGCAGATGTGCAATAGCAGTCCTTTACAGTGGTACGGAT
GAATTACAAGTTAACGATTGCCTCAATTATATTAGATCCATAAAACTAAA
TAAAAAACCTTTATATTGTGAACGATTTTGGTTGGCAAATGTTCATGTTG
ATGTTAGTCAACCTCCACAGAAGAAAGTTCGTGGTGTTGTGTAGTCATTT
TTTCGAATTGTTTAAAGTTTTCTTTTGACGTTTATCTGTATGGTTGCATT
TCTACTATTTATTGTAAATATACAGTTTATTGTTTTGGCCATTTCTTTTT
TTTGCTTTTGTGATCATTTTAATTGAAAAAAATGTATTTATTGTTTAGAG
AAAATGTAATTGGTTAATTTTTATTATTCGATTTGTG
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protein sequence of SMED30007922-orf-1

>SMED30007922-orf-1 ID=SMED30007922-orf-1|Name=SMED30007922-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=644bp
MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPT
PSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLS
YLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNT
SMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSES
YMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYI
PKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTE
DEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLS
TQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISS
RDTEHIPRLVLNLPETKKRCVENSINGDIESNRRLISNIKLSKPPSEGWA
LMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPD
CEELLWEKIVSKLATKNILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFI
FIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQV
NDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKKVRGVV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002056medial muscle cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell