SMED30007879

Overview


Name	SMED30007879
Smed ID	SMED30007879
Length (bp)	964

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30007879 (SMED30007879) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007879 (SMED30007879) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007879 (SMED30007879) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007879 (SMED30007879) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30007879

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 8

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
anterior side	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30007879	SMESG000014356.1	dd_Smed_v4_4641_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30007879 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.002641:19387..20401 -	4724

Homology

[Close]

BLAST of SMED30007879 vs. Ensembl Human
Match: PPP1R3B (protein phosphatase 1 regulatory subunit 3B [Source:HGNC Symbol;Acc:HGNC:14942])

HSP 1 Score: 48.1358 bits (113), Expect = 3.766e-8
Identity = 47/160 (29.38%), Postives = 76/160 (47.50%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV   +  S    D  + ++  HV L   V+ D  I G + V N   +KTV +R + + W S+ D+  ++   T++ S  D FSF I +      +   +EFA+ Y
Sbjct:   58 KVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAES--ESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVYY 214

HSP 2 Score: 28.8758 bits (63), Expect = 3.766e-8
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR++++
Sbjct:  215 ECNGQTYWDSNRGKNYRIIRA 235

[Close]

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV   +  S    D  + ++  HV L   V+ D  I G + V N   +KTV +R + + W S+ D+  ++   T++ S  D FSF I +      +   +EFA+ Y
Sbjct:   58 KVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAES--ESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVYY 214

HSP 2 Score: 28.8758 bits (63), Expect = 3.766e-8
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR++++
Sbjct:  215 ECNGQTYWDSNRGKNYRIIRA 235

[Close]

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV   +  S    D  + ++  HV L   V+ D  I G + V N   +KTV +R + + W S+ D+  ++   T++ S  D FSF I +      +   +EFA+ Y
Sbjct:   58 KVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAES--ESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVYY 214

HSP 2 Score: 28.8758 bits (63), Expect = 3.766e-8
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR++++
Sbjct:  215 ECNGQTYWDSNRGKNYRIIRA 235

[Close]

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV   +  S    D  + ++  HV L   V+ D  I G + V N   +KTV +R + + W S+ D+  ++   T++ S  D FSF I +      +   +EFA+ Y
Sbjct:   58 KVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAES--ESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVYY 214

HSP 2 Score: 28.8758 bits (63), Expect = 3.766e-8
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR++++
Sbjct:  215 ECNGQTYWDSNRGKNYRIIRA 235

[Close]

BLAST of SMED30007879 vs. Ensembl Celegans
Match: H18N23.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:Q400N3])

HSP 1 Score: 49.2914 bits (116), Expect = 4.848e-7
Identity = 53/184 (28.80%), Postives = 81/184 (44.02%), Query Frame = 2

Query:  278 LSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLKKFRSNT----------PSP-------DIFPDFV-YTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQICV-SSVCCVFGAVIEFAICYNV 772
            L   E  K RKSV FAD+ G E+ +    T      P    S L+++R  +          P+P           D+V +  N E+       + V L++V L+        + G I V N   +K+VFVR + N WIS++D  + +  + S   D F F + + SSV  +    IEF IC+  
Sbjct:  105 LELKEEEKLRKSVRFADDCGHELYMVRVATEPSNCPPKLSPSVLRRYRGESFEEEEMTTREPAPVWNLMFKQPAADYVRFRENMET-------NKVSLENVILNNEAYK---VMGTIKVGNIAFEKSVFVRYTMNGWISYMDKAAIYQPSTSKIQDTFKFDLDLPSSVEKIH--QIEFCICFKA 276

HSP 2 Score: 22.7126 bits (47), Expect = 4.848e-7
Identity = 7/13 (53.85%), Postives = 10/13 (76.92%), Query Frame = 1

Query:  775 GQEFWDNNNDINY 813
            G E+WD+N+  NY
Sbjct:  278 GTEYWDSNSGTNY 290

[Close]

BLAST of SMED30007879 vs. Ensembl Celegans
Match: H18N23.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:Q400N3])

HSP 1 Score: 49.2914 bits (116), Expect = 4.953e-7
Identity = 53/184 (28.80%), Postives = 81/184 (44.02%), Query Frame = 2

Query:  278 LSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLKKFRSNT----------PSP-------DIFPDFV-YTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQICV-SSVCCVFGAVIEFAICYNV 772
            L   E  K RKSV FAD+ G E+ +    T      P    S L+++R  +          P+P           D+V +  N E+       + V L++V L+        + G I V N   +K+VFVR + N WIS++D  + +  + S   D F F + + SSV  +    IEF IC+  
Sbjct:  127 LELKEEEKLRKSVRFADDCGHELYMVRVATEPSNCPPKLSPSVLRRYRGESFEEEEMTTREPAPVWNLMFKQPAADYVRFRENMET-------NKVSLENVILNNEAYK---VMGTIKVGNIAFEKSVFVRYTMNGWISYMDKAAIYQPSTSKIQDTFKFDLDLPSSVEKIH--QIEFCICFKA 298

HSP 2 Score: 22.7126 bits (47), Expect = 4.953e-7
Identity = 7/13 (53.85%), Postives = 10/13 (76.92%), Query Frame = 1

Query:  775 GQEFWDNNNDINY 813
            G E+WD+N+  NY
Sbjct:  300 GTEYWDSNSGTNY 312

[Close]

BLAST of SMED30007879 vs. Ensembl Celegans
Match: H18N23.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:Q400N3])

HSP 1 Score: 48.9062 bits (115), Expect = 5.829e-7
Identity = 53/184 (28.80%), Postives = 81/184 (44.02%), Query Frame = 2

Query:  278 LSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLKKFRSNT----------PSP-------DIFPDFV-YTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQICV-SSVCCVFGAVIEFAICYNV 772
            L   E  K RKSV FAD+ G E+ +    T      P    S L+++R  +          P+P           D+V +  N E+       + V L++V L+        + G I V N   +K+VFVR + N WIS++D  + +  + S   D F F + + SSV  +    IEF IC+  
Sbjct:  115 LELKEEEKLRKSVRFADDCGHELYMVRVATEPSNCPPKLSPSVLRRYRGESFEEEEMTTREPAPVWNLMFKQPAADYVRFRENMET-------NKVSLENVILNNEAYK---VMGTIKVGNIAFEKSVFVRYTMNGWISYMDKAAIYQPSTSKIQDTFKFDLDLPSSVEKIH--QIEFCICFKA 286

HSP 2 Score: 22.7126 bits (47), Expect = 5.829e-7
Identity = 7/13 (53.85%), Postives = 10/13 (76.92%), Query Frame = 1

Query:  775 GQEFWDNNNDINY 813
            G E+WD+N+  NY
Sbjct:  288 GTEYWDSNSGTNY 300

[Close]

BLAST of SMED30007879 vs. Ensembl Zebrafish
Match: ppp1r3da (protein phosphatase 1, regulatory subunit 3Da [Source:ZFIN;Acc:ZDB-GENE-080204-75])

HSP 1 Score: 58.9214 bits (141), Expect = 1.574e-9
Identity = 50/176 (28.41%), Postives = 83/176 (47.16%), Query Frame = 2

Query:  275 SLSFTENIKSRKSVSFADNHGIEVD-IRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVK---LKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKF-CSTFSSS---VDQFSFQICVSSVCCVFGAVIEFAICYNVLA 778
            S S    +  R +V F D+ G++++ ++VF + + P +P++VL  L        S  +     Y     S+   D    V+    + V L +++  +  I GI+ V N   +K V VR S   W S  + ++ +  S F+S+    D F F + V       GAV+EFAICY V+ 
Sbjct:   75 SPSERRRLSRRANVRFVDSLGLDLENVKVFKSGEDPFIPEHVLFRLLMNAELASSKRMEISLPYLKPLFSIQPGDSSDFVERLCFQQVCLERVLCYEPGIIGIVQVVNLAFEKEVSVRYSFTNWKSCSETKAYWVASKFNSNGPCCDTFRFHLPVPPFILHPGAVLEFAICYKVMG 250

[Close]

BLAST of SMED30007879 vs. Ensembl Zebrafish
Match: ppp1r3db (protein phosphatase 1, regulatory subunit 3Db [Source:ZFIN;Acc:ZDB-GENE-110201-1])

HSP 1 Score: 49.2914 bits (116), Expect = 2.841e-6
Identity = 44/161 (27.33%), Postives = 74/161 (45.96%), Query Frame = 2

Query:  314 VSFADNHGIEV-DIRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVY-THNFESVIDKDLDSIVKL---KHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKF-CSTFSSSV--DQFSFQICVSSVCCVFGAVIEFAICYNV 772
            V F D+ G+++ D++ F +++ P VP +V++ L          ++     Y   +F   +  D+ +  +    + V L Q+   +  I G   V N C +K V VR S   W S  D  + + CS     +  D F F + V       GAV++FAIC+ V
Sbjct:  100 VRFVDSLGLQLEDVKFFKSSEDPLVPVHVITRLLASSELASKKNLELSLPYFQPSFPDNMTTDVSAFSRRLCRQRVCLEQVFCSELGITGTAQVLNLCFQKEVIVRYSFTDWRSSADCRAGWVCSVRRGDLESDVFRFLLPVPPFIMQPGAVLQFAICFRV 260

[Close]

BLAST of SMED30007879 vs. Ensembl Zebrafish
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 43.1282 bits (100), Expect = 8.619e-6
Identity = 48/174 (27.59%), Postives = 77/174 (44.25%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVDI-RVFDTNQLP-EVPD----YVLSSLKKFRS--------NTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICYNV 772
            +++K VSFAD+ G+ + + ++F     P ++PD    +  SSL             + PS D          F   I+ D        HV L   ++ +  I G + V N   +K+V +R + N W +  D E ++   T++ S  D FSF+  +          IEFAICY V
Sbjct:   63 RAKKHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFTSSLTVSEGKDKLTLDFDQPSADYL-------KFRQRIEND--------HVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQVPSH-ERIEFAICYEV 220

HSP 2 Score: 25.409 bits (54), Expect = 8.619e-6
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRVVKS 828
            G   WDNN   NYR+++S
Sbjct:  222 GDTLWDNNQGKNYRIIQS 239

[Close]

Query:  299 KSRKSVSFADNHGIEVDI-RVFDTNQLP-EVPD----YVLSSLKKFRS--------NTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICYNV 772
            +++K VSFAD+ G+ + + ++F     P ++PD    +  SSL             + PS D          F   I+ D        HV L   ++ +  I G + V N   +K+V +R + N W +  D E ++   T++ S  D FSF+  +          IEFAICY V
Sbjct:   63 RAKKHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFTSSLTVSEGKDKLTLDFDQPSADYL-------KFRQRIEND--------HVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQVPSH-ERIEFAICYEV 220

HSP 2 Score: 25.409 bits (54), Expect = 8.619e-6
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRVVKS 828
            G   WDNN   NYR+++S
Sbjct:  222 GDTLWDNNQGKNYRIIQS 239

[Close]

Query:  299 KSRKSVSFADNHGIEVDI-RVFDTNQLP-EVPD----YVLSSLKKFRS--------NTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICYNV 772
            +++K VSFAD+ G+ + + ++F     P ++PD    +  SSL             + PS D          F   I+ D        HV L   ++ +  I G + V N   +K+V +R + N W +  D E ++   T++ S  D FSF+  +          IEFAICY V
Sbjct:   63 RAKKHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFTSSLTVSEGKDKLTLDFDQPSADYL-------KFRQRIEND--------HVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQVPSH-ERIEFAICYEV 220

HSP 2 Score: 25.409 bits (54), Expect = 8.619e-6
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRVVKS 828
            G   WDNN   NYR+++S
Sbjct:  222 GDTLWDNNQGKNYRIIQS 239

[Close]

BLAST of SMED30007879 vs. Ensembl Xenopus
Match: nbl1 (NBL1, DAN family BMP antagonist [Source:Xenbase;Acc:XB-GENE-479181])

HSP 1 Score: 61.2326 bits (147), Expect = 2.280e-10
Identity = 45/157 (28.66%), Postives = 76/157 (48.41%), Query Frame = 2

Query:  308 KSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDV--IYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            + VSFAD  G + V ++ FDT ++P     ++S L  +   T   +   +F  T +F      D+   V+ K V L  + +      + GII V N   +K  +VR S + W ++ D  +++   +F+   DQF+F I +       GA ++F ICY
Sbjct:   63 RKVSFADAFGFDLVSVKEFDTWEVP-----IVSQLLSYEVET---NYIEEFYLTASFTLPSADDIIGKVQAKKVLLESIDMTPGTSSMKGIIRVLNVSYEKQTYVRMSLDDWQTYYDLTAEYIPYSFNGDTDQFAFTITLVPPYQKEGASVQFCICY 211

HSP 2 Score: 23.483 bits (49), Expect = 2.280e-10
Identity = 7/13 (53.85%), Postives = 10/13 (76.92%), Query Frame = 1

Query:  784 FWDNNNDINYRVV 822
            FWDNN+  NY ++
Sbjct:  218 FWDNNDGQNYTLI 230

[Close]

BLAST of SMED30007879 vs. Ensembl Xenopus
Match: ppp1r3b (Xenopus tropicalis protein phosphatase 1 regulatory subunit 3B (ppp1r3b), mRNA. [Source:RefSeq mRNA;Acc:NM_001015950])

HSP 1 Score: 44.2838 bits (103), Expect = 3.266e-7
Identity = 60/225 (26.67%), Postives = 103/225 (45.78%), Query Frame = 2

Query:  134 KVKQIDKCTKSALNSDSTHVSKSE-RLSRKEQCDRLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIEVD-IRVF----DTNQLP----EVPDYV--LSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFC-STFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K   +D   K  L S      + E R++RK          T +      LS  EN K +K VSFAD+ G+ +  ++V+    D  ++P    E+ D +  L++++K R        F DFV      S    D  + ++ + V L   ++ D  + G + V N   +K+V +R + + W ++ DY+  +   T++ S  D FSF + +        A IEFA+C+
Sbjct:   31 KTMAVDIAMKFYLRSPPLRRDRVECRIARKSNEPLRPCIQTTNKTLLSELSNQEN-KVKKRVSFADSRGLALTMVKVYSDFDDELEIPFNISELIDNIVNLTTVEKER-------FFLDFVQP----SADYLDFRNRLQAESVCLENCMLKDKALVGTVKVKNLAFQKSVKIRMTCDSWQTYKDYDCLYVKDTYAGSDKDTFSFDVSLPE-GIQSSASIEFAVCF 242

HSP 2 Score: 29.6462 bits (65), Expect = 3.266e-7
Identity = 10/21 (47.62%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  763 LQCAGQEFWDNNNDINYRVVK 825
             +C G+ FWD+N  +NYR+V+
Sbjct:  242 FECEGRIFWDSNKGLNYRIVR 262

[Close]

BLAST of SMED30007879 vs. Ensembl Xenopus
Match: ppp1r3b (Xenopus tropicalis protein phosphatase 1 regulatory subunit 3B (ppp1r3b), mRNA. [Source:RefSeq mRNA;Acc:NM_001015950])

HSP 1 Score: 44.2838 bits (103), Expect = 3.379e-7
Identity = 60/225 (26.67%), Postives = 103/225 (45.78%), Query Frame = 2

Query:  134 KVKQIDKCTKSALNSDSTHVSKSE-RLSRKEQCDRLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIEVD-IRVF----DTNQLP----EVPDYV--LSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K   +D   K  L S      + E R++RK          T +      LS  EN K +K VSFAD+ G+ +  ++V+    D  ++P    E+ D +  L++++K R        F DFV      S    D  + ++ + V L   ++ D  + G + V N   +K+V +R + + W ++ DY+  +   T++ S  D FSF + +        A IEFA+C+
Sbjct:   20 KTMAVDIAMKFYLRSPPLRRDRVECRIARKSNEPLRPCIQTTNKTLLSELSNQEN-KVKKRVSFADSRGLALTMVKVYSDFDDELEIPFNISELIDNIVNLTTVEKER-------FFLDFVQP----SADYLDFRNRLQAESVCLENCMLKDKALVGTVKVKNLAFQKSVKIRMTCDSWQTYKDYDCLYVKDTYAGSDKDTFSFDVSLPE-GIQSSASIEFAVCF 231

HSP 2 Score: 29.6462 bits (65), Expect = 3.379e-7
Identity = 10/21 (47.62%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  763 LQCAGQEFWDNNNDINYRVVK 825
             +C G+ FWD+N  +NYR+V+
Sbjct:  231 FECEGRIFWDSNKGLNYRIVR 251

[Close]

BLAST of SMED30007879 vs. Ensembl Mouse
Match: Ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:MGI Symbol;Acc:MGI:2177268])

HSP 1 Score: 50.8322 bits (120), Expect = 5.270e-9
Identity = 48/160 (30.00%), Postives = 75/160 (46.88%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV      S    D  + ++  HV L   V+ D  I G + V N   +K V +R + + W SF D+  ++   T++ S  D FSF I +      +   +EFA+CY
Sbjct:   57 KVKKRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAES--ESFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVCY 213

HSP 2 Score: 28.4906 bits (62), Expect = 5.270e-9
Identity = 9/21 (42.86%), Postives = 15/21 (71.43%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR+ ++
Sbjct:  214 ECNGQAYWDSNKGKNYRITRA 234

[Close]

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV      S    D  + ++  HV L   V+ D  I G + V N   +K V +R + + W SF D+  ++   T++ S  D FSF I +      +   +EFA+CY
Sbjct:   57 KVKKRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAES--ESFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVCY 213

HSP 2 Score: 28.4906 bits (62), Expect = 5.270e-9
Identity = 9/21 (42.86%), Postives = 15/21 (71.43%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR+ ++
Sbjct:  214 ECNGQAYWDSNKGKNYRITRA 234

[Close]

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV      S    D  + ++  HV L   V+ D  I G + V N   +K V +R + + W SF D+  ++   T++ S  D FSF I +      +   +EFA+CY
Sbjct:   57 KVKKRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAES--ESFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVCY 213

HSP 2 Score: 28.4906 bits (62), Expect = 5.270e-9
Identity = 9/21 (42.86%), Postives = 15/21 (71.43%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR+ ++
Sbjct:  214 ECNGQAYWDSNKGKNYRITRA 234

[Close]

BLAST of SMED30007879 vs. Ensembl Mouse
Match: Ppp1r3a (protein phosphatase 1, regulatory subunit 3A [Source:MGI Symbol;Acc:MGI:2153588])

HSP 1 Score: 48.1358 bits (113), Expect = 1.144e-6
Identity = 49/171 (28.65%), Postives = 73/171 (42.69%), Query Frame = 2

Query:  308 KSVSFADNHGIE-VDIRVFDTNQLPEVP-DYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIY-----------GIINVFNFCPKKTVFVRCSTNKWISFIDYESKFC-STFSSSVDQFSFQICVSSVCCVFGAVIEFAICYNVLA 778
            + VSFAD+ G   V ++ FD  +LP V  D+ LS            D+F      H  E V+    D +   K   + QL +   V+            GII V N   +K V+VR S + W +  D  +++  ++     DQFSF+I +       G  +EF I Y   A
Sbjct:   64 RRVSFADSLGFSLVSVKEFDCWELPSVSTDFDLSG-----------DVF------HTDEYVLSPLFD-LPSSKEKLMEQLQVQKAVLESAEHLPGSSMKGIIRVLNISFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKISLVPPYQKEGGKVEFCIRYETSA 216

HSP 2 Score: 23.483 bits (49), Expect = 1.144e-6
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 1

Query:  784 FWDNNNDINYRVV 822
            FW NNN  NY +V
Sbjct:  219 FWSNNNGTNYILV 231

[Close]

BLAST of SMED30007879 vs. UniProt/SwissProt
Match: sp|A6QNP3|PPR3B_BOVIN (Protein phosphatase 1 regulatory subunit 3B OS=Bos taurus OX=9913 GN=PPP1R3B PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.073e-8
Identity = 50/161 (31.06%), Postives = 76/161 (47.20%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICYN 769
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV   +  S    D  + ++  HV L   V+ D  I G + V N   +KTV VR + + W SF D+   +   T++ S  D FSF I +      +   +EFA+CY 
Sbjct:   57 KVKKRVSFADNQGLALTMVKVFSEFDDPLDIPLNITELLDSIVSLTTAES--ESFVLDFSQPSADYLDFRNRLQTDHVCLENCVLKDRSIAGTVKVQNLAFEKTVKVRMTFDTWKSFTDFPCWYVKDTYAGSDKDTFSFDISLPEKIQSY-ERMEFAVCYE 214

HSP 2 Score: 29.6462 bits (65), Expect = 1.073e-8
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR++++
Sbjct:  214 ECNGQTYWDSNKGKNYRIIRA 234

[Close]

BLAST of SMED30007879 vs. UniProt/SwissProt
Match: sp|Q8C767|PPR3B_MOUSE (Protein phosphatase 1 regulatory subunit 3B OS=Mus musculus OX=10090 GN=Ppp1r3b PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.108e-8
Identity = 48/160 (30.00%), Postives = 75/160 (46.88%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV      S    D  + ++  HV L   V+ D  I G + V N   +K V +R + + W SF D+  ++   T++ S  D FSF I +      +   +EFA+CY
Sbjct:   57 KVKKRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAES--ESFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVCY 213

HSP 2 Score: 28.4906 bits (62), Expect = 3.108e-8
Identity = 9/21 (42.86%), Postives = 15/21 (71.43%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR+ ++
Sbjct:  214 ECNGQAYWDSNKGKNYRITRA 234

[Close]

BLAST of SMED30007879 vs. UniProt/SwissProt
Match: sp|Q6IN01|PPR3B_RAT (Protein phosphatase 1 regulatory subunit 3B OS=Rattus norvegicus OX=10116 GN=Ppp1r3b PE=1 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 6.244e-8
Identity = 47/160 (29.38%), Postives = 75/160 (46.88%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV      S    D  + ++  HV L   V+ +  I G + V N   +K V +R + + W SF D+  ++   T++ S  D FSF I +      +   +EFA+CY
Sbjct:   57 KVKKRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAES--ESFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKEKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVCY 213

HSP 2 Score: 28.8758 bits (63), Expect = 6.244e-8
Identity = 9/21 (42.86%), Postives = 15/21 (71.43%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR+ ++
Sbjct:  214 ECNGQSYWDSNKGKNYRITRA 234

[Close]

BLAST of SMED30007879 vs. UniProt/SwissProt
Match: sp|Q86XI6|PPR3B_HUMAN (Protein phosphatase 1 regulatory subunit 3B OS=Homo sapiens OX=9606 GN=PPP1R3B PE=1 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 1.612e-7
Identity = 47/160 (29.38%), Postives = 76/160 (47.50%), Query Frame = 2

Query:  299 KSRKSVSFADNHGIEVD-IRVFDTNQLP-EVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSS-VDQFSFQICVSSVCCVFGAVIEFAICY 766
            K +K VSFADN G+ +  ++VF     P ++P  +   L    S T +      FV   +  S    D  + ++  HV L   V+ D  I G + V N   +KTV +R + + W S+ D+  ++   T++ S  D FSF I +      +   +EFA+ Y
Sbjct:   58 KVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAES--ESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQSY-ERMEFAVYY 214

HSP 2 Score: 28.8758 bits (63), Expect = 1.612e-7
Identity = 9/21 (42.86%), Postives = 16/21 (76.19%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKS 828
            +C GQ +WD+N   NYR++++
Sbjct:  215 ECNGQTYWDSNRGKNYRIIRA 235

[Close]

BLAST of SMED30007879 vs. UniProt/SwissProt
Match: sp|Q00756|PPR3A_RABIT (Protein phosphatase 1 regulatory subunit 3A OS=Oryctolagus cuniculus OX=9986 GN=PPP1R3A PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 6.040e-7
Identity = 45/160 (28.12%), Postives = 74/160 (46.25%), Query Frame = 2

Query:  308 KSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDK-DLDSIVKLKHVFLH--QLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFC-STFSSSVDQFSFQICVSSVCCVFGAVIEFAICYNV 772
            + VSFADN G   V ++ FDT +LP V          F++         ++V +  F+    K DL   ++++   L   + V     + GII V N   +K V+VR S + W +  D  +++  ++     DQFSF+I +       G+ +EF I Y  
Sbjct:   64 RRVSFADNFGFNLVSVKEFDTWELPSVSTTFELGKDAFQTE--------EYVLSPLFDLPASKEDLMQQLQVQKAMLESTEYVPGSTSMKGIIRVLNISFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKISLVPPYQKDGSKVEFCIRYET 215

HSP 2 Score: 24.2534 bits (51), Expect = 6.040e-7
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 1

Query:  784 FWDNNNDINYRVV 822
            FW NNN  NY +V
Sbjct:  220 FWSNNNGTNYTLV 232

[Close]

BLAST of SMED30007879 vs. TrEMBL
Match: A0A3N0XRC7 (Protein phosphatase 1 regulatory subunit 3E OS=Anabarilius grahami OX=495550 GN=DPX16_20044 PE=4 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.472e-14
Identity = 58/189 (30.69%), Postives = 100/189 (52.91%), Query Frame = 2

Query:  233 RLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFR---------SNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            R K   T + +    ++  ++  S+K V FAD+ G++ + I+ FD + +PEVP+ ++   KK R         SNT S  +F + ++T N  S+ D  LD ++ LK V L  +   +  + GI+ V N   +K V++R S N W +F+D  + +   +     D+FSF+I ++     FG  ++FAI Y
Sbjct:   83 RCKSLPTPAERAKLEIARVQSPSSQKKVRFADSLGLDLISIKHFDDSDMPEVPERIIDKFKKARALHLNNFDKSNTSSQSVFMELLFT-NPGSLPDF-LDRVIALK-VLLESVQADEFSLSGIVRVLNLAFEKNVYLRYSLNNWTTFVDILASYVPHSNDGQTDKFSFKI-ITPTFLEFGGTLQFAIRY 267

HSP 2 Score: 28.8758 bits (63), Expect = 2.472e-14
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 1

Query:  769 CAGQEFWDNNNDINYRV 819
              G EFWDNNN  NYR+
Sbjct:  269 VGGGEFWDNNNGSNYRI 285

[Close]

BLAST of SMED30007879 vs. TrEMBL
Match: A0A498LU78 (Phosphatase 1 regulatory subunit 3E-like protein OS=Labeo rohita OX=84645 GN=ROHU_035300 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.138e-14
Identity = 60/192 (31.25%), Postives = 100/192 (52.08%), Query Frame = 2

Query:  224 QCDRLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFR---------SNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFS-SSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            Q  R K   T++ +    ++ + +  S+K V FAD+ G++ + I+ FD   +PEVPD ++   KK R         SNT S  +F + ++T N  S+ D  LD +   K V L  +   +  + GI+ VFN   +K V++R + N W +F+D  + +    S    D+FSF+I ++     FG  ++FAI Y
Sbjct:   73 QRRRCKSLPTSAERAKLEIARSRSPSSQKKVRFADSLGLDLISIKHFDDTDMPEVPDRIIDKFKKARALHLNNFDKSNTSSQSVFMELLFT-NPGSLPDF-LDRVTAAK-VLLETVQADEFSLSGIVRVFNLAFEKKVYLRYTLNNWSTFMDILASYVPHSSDGQTDKFSFKI-ITPTFLEFGGTLQFAIKY 260

HSP 2 Score: 28.4906 bits (62), Expect = 3.138e-14
Identity = 12/15 (80.00%), Postives = 12/15 (80.00%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRV 819
            G EFWDNNN  NYRV
Sbjct:  264 GGEFWDNNNGNNYRV 278

[Close]

BLAST of SMED30007879 vs. TrEMBL
Match: A0A553MRA9 (CBM21 domain-containing protein OS=Danionella translucida OX=623744 GN=DNTS_010289 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.856e-12
Identity = 58/190 (30.53%), Postives = 93/190 (48.95%), Query Frame = 2

Query:  224 QCDRLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFRS-------NTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFS-SSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            Q  R K   T++ +    ++ T +  S+K V FAD+ G+E + ++ FD   +PEVPD V+   +K R+          S  +F + ++T N  S+   D  S V    V L  +   +  + G++ V N   +K+V +R + N W SF D  + +    S    DQFSF+I   S    FG  ++FAI Y
Sbjct:   80 QRRRCKSLPTSAERAKLEIARTRSPSSQKKVRFADSLGLELISVKHFDDTDVPEVPDRVIDKFRKARALHLNNFDGASSQSVFVELLFT-NPGSL--PDFPSRVSAAKVSLESVQADEFSLAGLVRVLNLAFEKSVHLRYTLNNWSSFTDSLASYVPNSSDGQTDQFSFKIITPSF-LEFGGTLQFAIKY 265

HSP 2 Score: 26.5646 bits (57), Expect = 2.856e-12
Identity = 11/16 (68.75%), Postives = 12/16 (75.00%), Query Frame = 1

Query:  772 AGQEFWDNNNDINYRV 819
             G EFWDNN+  NYRV
Sbjct:  268 GGCEFWDNNSGDNYRV 283

[Close]

BLAST of SMED30007879 vs. TrEMBL
Match: A0A5C6NKQ2 (Protein phosphatase 1 regulatory subunit OS=Takifugu flavidus OX=433684 GN=D4764_02G0011830 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.577e-11
Identity = 57/191 (29.84%), Postives = 95/191 (49.74%), Query Frame = 2

Query:  233 RLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLK-----------KFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSV-DQFSFQICVSSVCCVFGAVIEFAICY 766
            R K   T + +    +S T +  S+K V FAD+ G+E + I+ FD  + PEVP+ +L+ L            KF    P+  +F +  +T+     +     S+ ++K V L  +   +  + GI+ V N   +K+VF+R S N WI+F+D  + +    S  V D+F F+I V+      G  ++FAI Y
Sbjct:   72 RCKSLPTPTERAKLEISRTRSPTSQKKVRFADSLGLELITIKHFDDTEEPEVPERILAKLSNGPLHLNTMDTKF-PRAPAQTMFMELQFTN--PGTLPGFEQSVREVK-VLLESVETDEFSLSGIVRVLNLAFEKSVFLRYSLNNWITFMDSLASYVPCSSDGVTDKFRFKI-VTPTYLDSGGTLQFAIRY 257

HSP 2 Score: 29.6462 bits (65), Expect = 1.577e-11
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  772 AGQEFWDNNNDINYRV 819
             G+EFWDNNN  NY+V
Sbjct:  260 GGEEFWDNNNGNNYKV 275

[Close]

BLAST of SMED30007879 vs. TrEMBL
Match: A0A4Z2BJ64 (Protein phosphatase 1 regulatory subunit OS=Takifugu bimaculatus OX=433685 GN=fugu_003479 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.985e-11
Identity = 57/191 (29.84%), Postives = 95/191 (49.74%), Query Frame = 2

Query:  233 RLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLK-----------KFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSV-DQFSFQICVSSVCCVFGAVIEFAICY 766
            R K   T + +    +S T +  S+K V FAD+ G+E + I+ FD  + PEVP+ +L+ L            KF    P+  +F +  +T+     +     S+ ++K V L  +   +  + GI+ V N   +K+VF+R S N WI+F+D  + +    S  V D+F F+I V+      G  ++FAI Y
Sbjct:   79 RCKSLPTPTERAKLEISRTRSPTSQKKVRFADSLGLELITIKHFDDTEEPEVPERILAKLSNGPLHLNTMDTKF-PRAPAQTMFMELQFTN--PGTLPGFEQSVREVK-VLLESVETDEFSLSGIVRVLNLAFEKSVFLRYSLNNWITFMDSLASYVPCSSDGVTDKFRFKI-VTPTYLDSGGTLQFAIRY 264

HSP 2 Score: 29.261 bits (64), Expect = 1.985e-11
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  772 AGQEFWDNNNDINYRV 819
             G+EFWDNNN  NY+V
Sbjct:  267 GGEEFWDNNNGNNYKV 282

[Close]

BLAST of SMED30007879 vs. Ensembl Cavefish
Match: ENSAMXT00000026535.2 (pep primary_assembly:Astyanax_mexicanus-2.0:18:5639658:5645568:1 gene:ENSAMXG00000025809.2 transcript:ENSAMXT00000026535.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 2.942e-12
Identity = 43/170 (25.29%), Postives = 83/170 (48.82%), Query Frame = 2

Query:  302 SRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFRS---------NTPSPDIFPDFVYTH--NFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSV-DQFSFQICVSSVCCVFGAVIEFAICYNV 772
            S+K V FAD+ G+E + ++ FD    PE+P+ V    +K R+            +  +F + ++++     S + +  D  V L+ V   +  +      G++ V N   +K+V +R + N W +F+D  + +    S  + D+F F+I V+ +    G  ++FA+ Y V
Sbjct:  113 SQKKVRFADSLGLELISVKHFDDADEPEIPERVTDRFRKARAMHLNTLGVPGASAQSVFMEVLFSNPCASPSFMQRVRDLRVSLERVDADEFCL-----SGLVRVLNMAFEKSVTLRYTINNWATFVDAPASYVPESSDGITDKFCFKI-VTPMFLEAGGTLQFAVKYRV 276

HSP 2 Score: 34.2686 bits (77), Expect = 2.942e-12
Identity = 18/41 (43.90%), Postives = 25/41 (60.98%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRVVK-SIAHNLS*LFVCLIEVIAR*VYL 894
            GQEFWDNN   NYRV    I H    L+V ++ ++ R V++
Sbjct:  278 GQEFWDNNGGSNYRVKPFYICHT---LYVSIVSIVCRTVWV 315

[Close]

BLAST of SMED30007879 vs. Ensembl Cavefish
Match: ppp1r3da (protein phosphatase 1 regulatory subunit 3D-like [Source:NCBI gene;Acc:103042623])

HSP 1 Score: 53.1434 bits (126), Expect = 2.372e-8
Identity = 49/169 (28.99%), Postives = 78/169 (46.15%), Query Frame = 2

Query:  305 RKSVSFADNHGIEVD-IRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKD----LDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYES-----KFCSTFSS--SVDQFSFQICVSSVCCVFGAVIEFAICYNVL 775
            R  V F D+ G++++ ++VF + + P VP++VL  L        S  +     Y     S    D    L+ + + K V L +++  +  I GI+ V N   +K V+VR S   W S  + ++     K+    S   S D F F + V       GAV+EFA+ Y VL
Sbjct:  131 RTGVRFVDSLGMDLENVKVFKSGEDPFVPEHVLFRLLMNAELAASKGLEISLPYLKPVFSEQPGDSANFLERLFRQK-VCLERVLCYEPGIIGIVQVLNLAFEKEVYVRYSFTNWKSCSETKANWVGNKYMEGLSDGCSCDSFRFHLPVPPFLLHPGAVLEFAVRYKVL 298

HSP 2 Score: 23.8682 bits (50), Expect = 2.372e-8
Identity = 8/14 (57.14%), Postives = 12/14 (85.71%), Query Frame = 1

Query:  781 EFWDNNNDINYRVV 822
            +FWDNN+  NY++V
Sbjct:  301 QFWDNNDGQNYKLV 314

[Close]

BLAST of SMED30007879 vs. Ensembl Cavefish
Match: ppp1r3db (protein phosphatase 1, regulatory subunit 3Db [Source:ZFIN;Acc:ZDB-GENE-110201-1])

HSP 1 Score: 47.7506 bits (112), Expect = 3.056e-7
Identity = 45/176 (25.57%), Postives = 78/176 (44.32%), Query Frame = 2

Query:  299 KSRKS--VSFADNHGIEVD-IRVFDTNQLPEVPDYVLSSL---------KKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKL--KHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQ-----FSFQICVSSVCCVFGAVIEFAICYN 769
            K+R+   V F D+ G+E++ ++ F  ++ P VP +V+  L         K      P         +  +F   +    D + +L  +HV L Q+   +  I G + V N   +K V VR S   W S  + ++ + ST   + D+     F F++ V       GA ++FAIC+ 
Sbjct:   78 KARRGSQVRFVDSLGLELERVKFFKASEDPTVPVHVICRLLASSELAFGKHLELTLP--------YFQPSFPENMASQPDFLQRLCRQHVCLEQVHCSELGIIGTVQVLNLAFEKDVTVRYSFTDWKSSAESKACWVSTLHRNGDEPESDIFRFRLPVPPFILKPGATLQFAICFQ 245

HSP 2 Score: 25.7942 bits (55), Expect = 3.056e-7
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVV 822
            Q  G E+WDNN+  NY++ 
Sbjct:  245 QSQGVEYWDNNDGHNYKLA 263

[Close]

BLAST of SMED30007879 vs. Ensembl Nematostella
Match: EDO47072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN47])

HSP 1 Score: 55.0694 bits (131), Expect = 1.102e-9
Identity = 27/71 (38.03%), Postives = 46/71 (64.79%), Query Frame = 2

Query:  557 VIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCS-TFSSSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            V+ G+I V+N   +K VFVR ST+ W S++D  ++F S +   ++D++ F++ ++ V    G  IEFA+CY
Sbjct:   32 VLVGLIRVWNIAYEKKVFVRYSTDNWSSYVDLPAEFMSQSHEGTIDKYVFKL-LAPVGLAVGDTIEFAVCY 101

[Close]

BLAST of SMED30007879 vs. Ensembl Nematostella
Match: EDO41629 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S413])

HSP 1 Score: 44.669 bits (104), Expect = 2.663e-7
Identity = 25/77 (32.47%), Postives = 37/77 (48.05%), Query Frame = 2

Query:  554 DVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKF-CSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICYNVLAK 781
            + I G I V N    K V VR + + W SF D  + +   +F  + D+FSF + +       G  I+FAIC+    K
Sbjct:   45 NTIMGTIKVKNISYHKEVLVRYTLDSWHSFTDVPASYVLDSFDGNSDRFSFAVTIPEYFMASGGNIDFAICFRADGK 121

HSP 2 Score: 27.335 bits (59), Expect = 2.663e-7
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRV 819
            G+EFWDNN+  NY+V
Sbjct:  120 GKEFWDNNSGQNYKV 134

[Close]

BLAST of SMED30007879 vs. Ensembl Nematostella
Match: EDO41624 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S401])

HSP 1 Score: 41.9726 bits (97), Expect = 2.927e-6
Identity = 31/113 (27.43%), Postives = 54/113 (47.79%), Query Frame = 2

Query:  431 PSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFID-YESKFCSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            P P   PDF         +++ L  +V L++ ++     ++  + G+I V N   +K VF R S + W++F D     F  T     D+F F++ +     V G  +EFA+C+
Sbjct:    8 PQPASRPDF---------LERMLTQMVCLENAYMGG--SLNVSLIGLIRVRNISYEKEVFARYSIDHWVNFQDCMADHFMQTNGGEFDKFVFKLVLPKDFYV-GGTVEFAVCF 108

HSP 2 Score: 26.1794 bits (56), Expect = 2.927e-6
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRVV 822
            GQE+WDNN+  NY V+
Sbjct:  112 GQEYWDNNDGNNYTVM 127

[Close]

BLAST of SMED30007879 vs. Ensembl Nematostella
Match: EDO48422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJS3])

HSP 1 Score: 43.8986 bits (102), Expect = 5.494e-6
Identity = 30/113 (26.55%), Postives = 52/113 (46.02%), Query Frame = 2

Query:  431 PSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFID-YESKFCSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            P P   PDF         +++    +V L++ ++     ++  + G+I V N   +K VF R S + WI F D   +    T     D+F F++ +   C   G  +EFA+C+
Sbjct:  106 PQPASSPDF---------LERMRTQMVCLENAYMGDSSNLE--LIGLIRVRNISYEKEVFARYSIDHWIHFQDCMAAHLLQTNEGKFDKFIFKLVLPK-CFYVGGTVEFAVCF 206

HSP 2 Score: 23.483 bits (49), Expect = 5.494e-6
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 1

Query:  775 GQEFWDNNNDINYRV 819
            GQE+WDNN   N+ V
Sbjct:  210 GQEYWDNNRGNNFTV 224

[Close]

BLAST of SMED30007879 vs. Ensembl Medaka
Match: ENSORLT00000011971.2 (protein phosphatase 1 regulatory subunit 3E [Source:NCBI gene;Acc:101160970])

HSP 1 Score: 57.3806 bits (137), Expect = 1.530e-10
Identity = 47/170 (27.65%), Postives = 81/170 (47.65%), Query Frame = 2

Query:  302 SRKSVSFADNHGIE-VDIRVFDTNQLPEVPDYVLSSLKKFR--------SNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSV-DQFSFQICVSSVCCVFGAVIEFAICYNVLAK 781
            S+K V FAD+ G+E + ++ F+    PEVP+ +L+ L K             PS  +  +  +T+          +  V+   V L  +   +  + G + V N   +K+V +R S N WI+F+D  + +    S  V D+F F+I + +     GA ++FAI Y V  +
Sbjct:   60 SQKKVRFADSLGLELISVKHFNDADDPEVPERILAKLHKGNLHWLYTKFPRAPSQPVLMELQFTN---PGTLPGFEQKVREVKVLLESVEADEFSLSGFVRVLNMAFEKSVSLRYSLNNWITFMDSLATYVPNSSDGVTDKFCFKIVMPTYLDNGGA-LQFAIKYCVGGR 225

HSP 2 Score: 26.9498 bits (58), Expect = 1.530e-10
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 1

Query:  769 CAGQEFWDNNNDINYRV 819
              G+EFWDNN+  NY+V
Sbjct:  222 VGGREFWDNNHGNNYKV 238

[Close]

BLAST of SMED30007879 vs. Ensembl Medaka
Match: ENSORLT00000017811.2 (pep primary_assembly:ASM223467v1:7:21121925:21128746:1 gene:ENSORLG00000014198.2 transcript:ENSORLT00000017811.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 6.275e-8
Identity = 41/167 (24.55%), Postives = 76/167 (45.51%), Query Frame = 2

Query:  296 IKSRKSVSFADNHGIE-VDIR---VFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDD---VIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFC-STFSSSVDQFSFQICVSSVCCVFGAVIEFAICYNV 772
            + + K VSFAD  G + VD+R    FD++   +   +      K+R     P   P +     F++     L  +V+   V +  +   +D      G+I V N    K V++R + + W ++ D+ +++   + +   DQFSF++C +      G+ IEF + Y  
Sbjct:   52 LAAGKRVSFADTTGGDLVDVREFVAFDSDDESDSAKWEHEQ-AKYRK----PHREPTYSLHPEFQTPSHGALLHVVRKNKVEVEHIAPSEDDPLAFRGVIRVLNVSFHKAVYIRSTMDDWATYFDHPAEYVQGSHNEDTDQFSFKLCFAPPFTNHGSRIEFVVRYET 213

HSP 2 Score: 22.3274 bits (46), Expect = 6.275e-8
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVV 822
            + A  +FW NN+ +NY V 
Sbjct:  212 ETAEGDFWANNSSLNYGVT 230

[Close]

BLAST of SMED30007879 vs. Ensembl Medaka
Match: ppp1r3db (protein phosphatase 1, regulatory subunit 3Db [Source:ZFIN;Acc:ZDB-GENE-110201-1])

HSP 1 Score: 44.2838 bits (103), Expect = 1.971e-6
Identity = 49/169 (28.99%), Postives = 75/169 (44.38%), Query Frame = 2

Query:  314 VSFADNHGIEVD-IRVFDTNQLPEVPDYVLSSL--KKFRSNTPSPDI-FPDF--VYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFC--------STFSSSVDQFSFQICVSSVCCVFGAVIEFAICYNVLA 778
            V F D  G++++ I+VF   + P VP++V+  L     R+    P++  P F   +  N  +   + L ++V  K V L QL   D  I G I V N   +K V V  S   W +  D  + +         S  +   D F F + V       GAV+EFA+ Y+V  
Sbjct:   58 VRFIDALGLDLEEIKVFRVQEHPLVPEHVIFRLLMNSERAFGKLPELNLPYFKACFPENM-AAQPEFLPNLVSRK-VCLEQLTCSDHGITGTIRVLNLAYEKEVKVLYSFTNWRTRTDTTACWVMRGHEGDSSISALDTDLFRFHLPVPPFILQPGAVLEFAVRYHVTG 224

HSP 2 Score: 26.1794 bits (56), Expect = 1.971e-6
Identity = 8/18 (44.44%), Postives = 12/18 (66.67%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRV 819
               G ++WDNNN  NY++
Sbjct:  221 HVTGHDYWDNNNGSNYKL 238

[Close]

BLAST of SMED30007879 vs. Planmine SMEST
Match: SMESG000014356.1 (SMESG000014356.1)

HSP 1 Score: 434.876 bits (1117), Expect = 2.683e-155
Identity = 216/223 (96.86%), Postives = 217/223 (97.31%), Query Frame = 2

Query:  113 MEKETFFKVKQIDKCTKSALNSDSTHVSKSERLSRKEQCDRLKVESTNSNQKPRSLSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICYNVLAK 781
            MEKETFFKVKQIDKCTKSALNSDSTHVSKSERLSRKEQCDRLKVESTNSNQK RSLSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLKKFRSN PSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICY    +
Sbjct:    1 MEKETFFKVKQIDKCTKSALNSDSTHVSKSERLSRKEQCDRLKVESTNSNQKLRSLSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLKKFRSNAPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICYKCAGQ 223

HSP 2 Score: 59.6918 bits (143), Expect = 3.273e-10
Identity = 26/27 (96.30%), Postives = 27/27 (100.00%), Query Frame = 1

Query:  766 QCAGQEFWDNNNDINYRVVKSIAHNLS 846
            +CAGQEFWDNNNDINYRVVKSIAHNLS
Sbjct:  219 KCAGQEFWDNNNDINYRVVKSIAHNLS 245

[Close]

BLAST of SMED30007879 vs. Planmine SMEST
Match: SMESG000033999.1 (SMESG000033999.1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.930e-9
Identity = 47/157 (29.94%), Postives = 75/157 (47.77%), Query Frame = 2

Query:  308 KSVSFADNHGIEV-DIR-VFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFES-VIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSV-DQFSFQICVSSVCCVFGAVIEFAICY 766
            K+V FAD  G+++ DIR VF+ +  P++P   +  L    S++ +        Y  NFE      D    V++ +V L      ++ + G I V N   +K V VR + N W S ID+ + +       V D+FSF   + + C   G  I+FAI +
Sbjct:  115 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLV-LDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISF 270

[Close]

BLAST of SMED30007879 vs. Planmine SMEST
Match: SMESG000018209.1 (SMESG000018209.1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.930e-9
Identity = 47/157 (29.94%), Postives = 75/157 (47.77%), Query Frame = 2

Query:  308 KSVSFADNHGIEV-DIR-VFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVYTHNFES-VIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSV-DQFSFQICVSSVCCVFGAVIEFAICY 766
            K+V FAD  G+++ DIR VF+ +  P++P   +  L    S++ +        Y  NFE      D    V++ +V L      ++ + G I V N   +K V VR + N W S ID+ + +       V D+FSF   + + C   G  I+FAI +
Sbjct:  115 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLV-LDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISF 270

[Close]

BLAST of SMED30007879 vs. Planmine SMEST
Match: SMESG000061419.1 (SMESG000061419.1)

HSP 1 Score: 54.299 bits (129), Expect = 2.782e-8
Identity = 46/182 (25.27%), Postives = 89/182 (48.90%), Query Frame = 2

Query:  242 VESTNSNQKPRSLSFTENIKSRKSVSFADNHGIEV-DIRVFDTNQLPEVPDYVLSSLKKFRSNTPSPDIFPDFVY-----THNFESVIDKDLDSIVKLKHVFLHQLVIMDDVIYGIINVFNFCPKKTVFVRCSTNKWISFID-YESKFCSTFSSSVDQFSFQICVSSVCCVFGAVIEFAICY 766
            ++ +N N++ +S    +  K RKSV FAD  G+++ +I+    + + + P    S++K    +           Y       + ++VI+  + + ++L+  ++  + +     +G I V N   +K V +R + N W+SF D Y S    + +  +D+FSF + V     V G+ IEFA+ Y
Sbjct:   99 IDFSNDNRRSKSQVNRKTDKCRKSVRFADALGLQLSEIKFIKNSDIEKAPVLTSSAIKDLHLDHNCHLAMQGIKYFCPCFKIDEKTVIENTMKNNIQLEKAYIQGMTL-----FGRIFVKNISYEKNVGIRITYNNWVSFKDIYASYILESSNGIIDKFSFSVVVPRSVIV-GSKIEFAVFY 274

HSP 2 Score: 21.9422 bits (45), Expect = 2.782e-8
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 1

Query:  778 QEFWDNNNDINYRVVKSIAHNL 843
            + FWDNN++ NY V++  A N+
Sbjct:  280 ETFWDNNSNQNY-VLECFAKNM 300

[Close]

BLAST of SMED30007879 vs. Planmine SMEST
Match: SMESG000078349.1 (SMESG000078349.1)

HSP 1 Score: 47.7506 bits (112), Expect = 9.686e-7
Identity = 28/78 (35.90%), Postives = 44/78 (56.41%), Query Frame = 2

Query:  560 IYGIINVFNFCPKKTVFVRCSTN-KWISFIDYESKFCSTF---------SSSVDQFSFQICVSSVCCVFGAVIEFAIC 763
            I GII + N C +K VFVR S++ +W SF D+E+K+ S+          SS VD+F FQ+ + ++       +EF + 
Sbjct:  150 ITGIIKLINLCFEKRVFVRLSSDSRWTSFSDFEAKYKSSSNEFLPDTNESSDVDEFEFQVSLPNMEVGEATNLEFVVG 227

HSP 2 Score: 23.483 bits (49), Expect = 9.686e-7
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 1

Query:  784 FWDNNNDINYRV 819
            +WDNNN+ NYR+
Sbjct:  236 YWDNNNEKNYRL 247

The following BLAST results are available for this feature:

BLAST of SMED30007879 vs. Ensembl Human
BLAST of SMED30007879 vs. Ensembl Celegans
BLAST of SMED30007879 vs. Ensembl Fly
BLAST of SMED30007879 vs. Ensembl Zebrafish
BLAST of SMED30007879 vs. Ensembl Xenopus
BLAST of SMED30007879 vs. Ensembl Mouse
BLAST of SMED30007879 vs. UniProt/SwissProt
BLAST of SMED30007879 vs. TrEMBL
BLAST of SMED30007879 vs. Ensembl Cavefish
BLAST of SMED30007879 vs. Ensembl Sea Lamprey
BLAST of SMED30007879 vs. Ensembl Yeast
BLAST of SMED30007879 vs. Ensembl Nematostella
BLAST of SMED30007879 vs. Ensembl Medaka
BLAST of SMED30007879 vs. Planmine SMEST

BLAST of SMED30007879 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
PPP1R3B	3.766e-8	29.38	protein phosphatase 1 regulatory subunit 3B [Sourc...	[more]
PPP1R3B	3.766e-8	29.38	protein phosphatase 1 regulatory subunit 3B [Sourc...	[more]
PPP1R3B	3.766e-8	29.38	protein phosphatase 1 regulatory subunit 3B [Sourc...	[more]
PPP1R3B	3.766e-8	29.38	protein phosphatase 1 regulatory subunit 3B [Sourc...	[more]

BLAST of SMED30007879 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
H18N23.2	4.848e-7	28.80	Protein phosphatase 1 regulatory subunit [Source:...	[more]
H18N23.2	4.953e-7	28.80	Protein phosphatase 1 regulatory subunit [Source:...	[more]
H18N23.2	5.829e-7	28.80	Protein phosphatase 1 regulatory subunit [Source:...	[more]

BLAST of SMED30007879 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30007879 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ppp1r3da	1.574e-9	28.41	protein phosphatase 1, regulatory subunit 3Da [Sou...	[more]
ppp1r3db	2.841e-6	27.33	protein phosphatase 1, regulatory subunit 3Db [Sou...	[more]
ppp1r3b	8.619e-6	27.59	protein phosphatase 1, regulatory subunit 3B [Sour...	[more]
ppp1r3b	8.619e-6	27.59	protein phosphatase 1, regulatory subunit 3B [Sour...	[more]
ppp1r3b	8.619e-6	27.59	protein phosphatase 1, regulatory subunit 3B [Sour...	[more]

BLAST of SMED30007879 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nbl1	2.280e-10	28.66	NBL1, DAN family BMP antagonist [Source:Xenbase;Ac...	[more]
ppp1r3b	3.266e-7	26.67	Xenopus tropicalis protein phosphatase 1 regulator...	[more]
ppp1r3b	3.379e-7	26.67	Xenopus tropicalis protein phosphatase 1 regulator...	[more]

BLAST of SMED30007879 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Ppp1r3b	5.270e-9	30.00	protein phosphatase 1, regulatory subunit 3B [Sour...	[more]
Ppp1r3b	5.270e-9	30.00	protein phosphatase 1, regulatory subunit 3B [Sour...	[more]
Ppp1r3b	5.270e-9	30.00	protein phosphatase 1, regulatory subunit 3B [Sour...	[more]
Ppp1r3a	1.144e-6	28.65	protein phosphatase 1, regulatory subunit 3A [Sour...	[more]

BLAST of SMED30007879 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|A6QNP3\|PPR3B_BOVIN	1.073e-8	31.06	Protein phosphatase 1 regulatory subunit 3B OS=Bos...	[more]
sp\|Q8C767\|PPR3B_MOUSE	3.108e-8	30.00	Protein phosphatase 1 regulatory subunit 3B OS=Mus...	[more]
sp\|Q6IN01\|PPR3B_RAT	6.244e-8	29.38	Protein phosphatase 1 regulatory subunit 3B OS=Rat...	[more]
sp\|Q86XI6\|PPR3B_HUMAN	1.612e-7	29.38	Protein phosphatase 1 regulatory subunit 3B OS=Hom...	[more]
sp\|Q00756\|PPR3A_RABIT	6.040e-7	28.13	Protein phosphatase 1 regulatory subunit 3A OS=Ory...	[more]

BLAST of SMED30007879 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3N0XRC7	2.472e-14	30.69	Protein phosphatase 1 regulatory subunit 3E OS=Ana...	[more]
A0A498LU78	3.138e-14	31.25	Phosphatase 1 regulatory subunit 3E-like protein O...	[more]
A0A553MRA9	2.856e-12	30.53	CBM21 domain-containing protein OS=Danionella tran...	[more]
A0A5C6NKQ2	1.577e-11	29.84	Protein phosphatase 1 regulatory subunit OS=Takifu...	[more]
A0A4Z2BJ64	1.985e-11	29.84	Protein phosphatase 1 regulatory subunit OS=Takifu...	[more]

BLAST of SMED30007879 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSAMXT00000026535.2	2.942e-12	25.29	pep primary_assembly:Astyanax_mexicanus-2.0:18:563...	[more]
ppp1r3da	2.372e-8	28.99	protein phosphatase 1 regulatory subunit 3D-like [...	[more]
ppp1r3db	3.056e-7	25.57	protein phosphatase 1, regulatory subunit 3Db [Sou...	[more]

BLAST of SMED30007879 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30007879 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30007879 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4


Match Name	E-value	Identity	Description
EDO47072	1.102e-9	38.03	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41629	2.663e-7	32.47	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41624	2.927e-6	27.43	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO48422	5.494e-6	26.55	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30007879 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSORLT00000011971.2	1.530e-10	27.65	protein phosphatase 1 regulatory subunit 3E [Sourc...	[more]
ENSORLT00000017811.2	6.275e-8	24.55	pep primary_assembly:ASM223467v1:7:21121925:211287...	[more]
ppp1r3db	1.971e-6	28.99	protein phosphatase 1, regulatory subunit 3Db [Sou...	[more]

BLAST of SMED30007879 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000014356.1	2.683e-155	96.86	SMESG000014356.1	[more]
SMESG000033999.1	2.930e-9	29.94	SMESG000033999.1	[more]
SMESG000018209.1	2.930e-9	29.94	SMESG000018209.1	[more]
SMESG000061419.1	2.782e-8	25.27	SMESG000061419.1	[more]
SMESG000078349.1	9.686e-7	35.90	SMESG000078349.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30007879 ID=SMED30007879|Name=SMED30007879|organism=Schmidtea mediterranea sexual|type=transcript|length=964bp
ACTATGCACACTTCTGTGTATCTTCTTAATGAAGCACATTTTTGCTGACC
AATCAATCAAATTTTGAACTTAATTTTGTACGTGACTTACCGATTTGAAT
GATATTTGGAAAATGGAGAAAGAAACATTTTTCAAAGTCAAACAAATCGA
CAAATGCACAAAGTCAGCACTGAATTCCGATTCCACTCACGTAAGCAAAT
CAGAAAGACTTTCTCGAAAAGAGCAATGTGACAGATTGAAAGTCGAATCG
ACAAATAGCAATCAAAAACCGAGATCTCTCTCTTTTACAGAAAACATAAA
AAGCCGAAAATCTGTCTCATTTGCTGATAATCACGGAATCGAAGTTGATA
TTCGAGTTTTTGACACTAACCAACTTCCGGAAGTGCCGGATTACGTTTTG
TCGAGTTTAAAAAAGTTTCGCAGCAACACCCCTTCTCCGGACATTTTCCC
TGACTTTGTTTACACTCACAACTTTGAATCCGTCATTGACAAAGATCTTG
ATAGTATTGTCAAATTAAAGCACGTATTTCTCCACCAATTAGTAATCATG
GACGACGTAATATATGGGATCATAAACGTCTTCAATTTCTGTCCGAAAAA
AACCGTTTTTGTTCGATGTAGCACAAATAAATGGATTTCATTTATTGATT
ACGAATCGAAATTTTGTTCAACCTTCTCATCGAGTGTCGATCAGTTTTCA
TTCCAAATTTGCGTTTCCTCCGTCTGCTGCGTTTTTGGGGCTGTAATCGA
ATTCGCAATCTGCTACAATGTGCTGGCCAAGAGTTCTGGGACAATAACAA
CGACATCAATTACAGAGTGGTCAAATCCATTGCTCACAATTTATCATGAT
TATTTGTTTGTTTGATTGAAGTAATTGCAAGATGAGTTTACTTATTTTCA
ATGACAATTTTTTTAAAAGTAAATCAATATTTTTAAATATCATATTTTTT
TGTTTGAAAAAAAA

protein sequence of SMED30007879-orf-1

>SMED30007879-orf-1 ID=SMED30007879-orf-1|Name=SMED30007879-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=251bp
MEKETFFKVKQIDKCTKSALNSDSTHVSKSERLSRKEQCDRLKVESTNSN
QKPRSLSFTENIKSRKSVSFADNHGIEVDIRVFDTNQLPEVPDYVLSSLK
KFRSNTPSPDIFPDFVYTHNFESVIDKDLDSIVKLKHVFLHQLVIMDDVI
YGIINVFNFCPKKTVFVRCSTNKWISFIDYESKFCSTFSSSVDQFSFQIC
VSSVCCVFGAVIEFAICYNVLAKSSGTITTTSITEWSNPLLTIYHDYLFV
*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000101	muscle cell
PLANA:0000140	anterior region of the whole animal

Vocabulary: **INTERPRO**
Term	Definition
IPR005036	CBM21_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **biological process**
Term	Definition
GO:0005975	carbohydrate metabolic process
GO:0005977	glycogen metabolic process

Main menu

SMED30007879

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 8

Owlery

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