SMED30007830

Overview
NameSMED30007830
Smed IDSMED30007830
Length (bp)1994
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007830 (SMED30007830) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007830 (SMED30007830) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007830 (SMED30007830) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007830 (SMED30007830) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007830

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30007830SMESG000047325.1 SmedASXL_015486SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30007830SMESG000047325.1 SmedASXL_015486SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
posterior muscle cellSMED30007830SMESG000047325.1 dd_Smed_v4_4889_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30007830SMESG000047325.1 dd_Smed_v4_4889_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30007830 vs. Planmine SMEST
Match: SMESG000047325.1 (SMESG000047325.1)

HSP 1 Score: 1307.35 bits (3382), Expect = 0.000e+0
Identity = 630/631 (99.84%), Postives = 631/631 (100.00%), Query Frame = 2
Query:   38 MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKDKNNAEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDSSQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSKAFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGVASTFGLLDTVRKTYLRGKPIYADVIKLASINFETPNKKRKMYTDQC 1930
            MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKDKN+AEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDSSQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSKAFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGVASTFGLLDTVRKTYLRGKPIYADVIKLASINFETPNKKRKMYTDQC
Sbjct:    1 MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKDKNSAEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDSSQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSKAFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGVASTFGLLDTVRKTYLRGKPIYADVIKLASINFETPNKKRKMYTDQC 631          
BLAST of SMED30007830 vs. Planmine SMEST
Match: SMESG000070344.1 (SMESG000070344.1)

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 299/644 (46.43%), Postives = 426/644 (66.15%), Query Frame = 2
Query:   38 MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKD-----KNNAEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDS------SQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSK-AFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRD--VDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGV--ASTFGLLDTVRKTYLRGKPIYADVIKLASINFET---PNKKRK 1912
            MNTD IYV NIPS+ KPVLLLHELENKW+RMLYLEN+FSG+RDAFIYNPT    HF +G+QKLR+K+ +  K L+V YVWFPP NVF+TTMIP+GASMLSY+FVENL K+    E+   LP Q  D+K WDIPER DKC++IKLP++SN +MRS++LGT+R+R ++G FP   E+IIS+   LS  M P++    ++ ++Y++NF +L++IPSKL VK L+++ K +NIKGCRVFF+ET N  K GF Y+P++LP+ +F++LFEKL S E+ L  G   +I + E WFQP  AEPP + +     +N  E +LT YL+L  IP N VA+QL +  PC +KL +F VN+LSTQIGF+V+RGL+     ++I+HFR+L LGG  I VDE+++ C++LP + S R  + IPR +  LP+ TKKRC+ +S      S +    +I+LS+ P E   LMK++FIC+ NLP  +S++A+IFDI     GCV+    E    AF+Y P+C    W+ I++K + K   G   +++EVYLQ+ D  + +   +F ++  IF YN+      D   +F  D  +I  +  ++T  GS+ A  +   G         L+ +R   L  KP+Y +   LA+++ +    P KK +
Sbjct:    1 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLP-DISSRDTEHIPRLVLNLPE-TKKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKTILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYND--VIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKKVR 640          
BLAST of SMED30007830 vs. Planmine SMEST
Match: SMESG000070604.1 (SMESG000070604.1)

HSP 1 Score: 558.91 bits (1439), Expect = 0.000e+0
Identity = 287/640 (44.84%), Postives = 409/640 (63.91%), Query Frame = 2
Query:   38 MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKDKNN-----AEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDS------SQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSK-AFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGV--ASTFGLLDTVRKTYLRGKPIYADVIKLASINFET---PNKK 1906
            MNTD +YV NIPS  KPVLLLHELENKW+RMLYLEN+FSG+RDA IYNP      F  G+Q+LR K+ D  K L+V YVWFPP NVF+TTMIP+GAS+LSYIFVEN HK+    E+  DLPLQ  D+K WDIPER DKC++IKLP++S+ AMRS++LGT+R+R ++G FPR  E+IIS    LS  M P++I+  ++ ++ ++NF +LH+IPSKL VK L+++ K +NIKGCRVFF+ET N +K GF+Y+P++LP+ +F+ LFEKL + E+T   G  +RI + E WFQP  A+PP + D+        E VL +YL+L  IP N VA+QL +  P  +KL +F VN+LSTQIGF+V+RGLS     ++I HFRNL +GG  I VDE+++ C+NL  +   R  + IPR  +    +  KR I +S      S K     I+LS+ P E   LMK+EFIC+ NLP +M+++A++F+I     GCV+    +    AF+Y P+C    W+ +I+K + K   G   +++E+YLQ+ D  +     ++  +  IF YN        E   +   LI  +  ++T NG +        G         L+ +R   ++GKP++A+++  A +  +    P KK
Sbjct:    1 MNTDLLYVSNIPSTMKPVLLLHELENKWSRMLYLENSFSGVRDALIYNPLSSIQIFYNGVQRLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENFHKEGNVAELTADLPLQQHDIKIWDIPERQDKCLVIKLPLSSSTAMRSYVLGTLRNRKVKGYFPRVYEMIISNTIELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNL-ADIETRDAEYIPRPQST--RVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKK 637          
BLAST of SMED30007830 vs. Planmine SMEST
Match: SMESG000070322.1 (SMESG000070322.1)

HSP 1 Score: 557.755 bits (1436), Expect = 0.000e+0
Identity = 287/640 (44.84%), Postives = 408/640 (63.75%), Query Frame = 2
Query:   38 MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKDKNN-----AEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDS------SQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSK-AFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGV--ASTFGLLDTVRKTYLRGKPIYADVIKLASINFET---PNKK 1906
            MNTD +YV NIPS  KPVLLLHELENKW+RMLYLEN+FSG+RDA IYNP      F  G+Q+LR K+ D  K L+V YVWFPP NVF+TTMIP+GAS+LSYIFVEN HK+    E+  DLPLQ  D+K WDIPER DKC++IKLP++S+ AMRS++LGT+R+R ++G FPR  E+IIS    LS  M P++I+  ++ ++ ++NF +LH+IPSKL VK L+++ K +NIKGCRVFF+ET N +K GF+Y+P++LP+ +F+ LFEKL + E+T   G  +RI + E WFQP  A+PP + D+        E VL +YL+L  IP N VA+QL +  P  +KL +F VN+LSTQIGF+V+RGLS     ++I HFRNL +GG  I VDE+++ C+NL  +   R  + IPR  +    +  KR I +S      S K     I+LS+ P E   LMK+EFIC+ NLP +M+++A++F+I     GCV+    +    AF+Y P+C    W+ +I K + K   G   +++E+YLQ+ D  +     ++  +  IF YN        E   +   LI  +  ++T NG +        G         L+ +R   ++GKP++A+++  A +  +    P KK
Sbjct:    1 MNTDLLYVSNIPSTMKPVLLLHELENKWSRMLYLENSFSGVRDALIYNPLSSIQIFYNGVQRLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENFHKEGNVAELTADLPLQQHDIKIWDIPERQDKCLVIKLPLSSSTAMRSYVLGTLRNRKVKGYFPRVYEMIISNTIELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNL-ADIETRDAEYIPRPQST--RVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKK 637          
BLAST of SMED30007830 vs. Planmine SMEST
Match: SMESG000017548.1 (SMESG000017548.1)

HSP 1 Score: 539.265 bits (1388), Expect = 0.000e+0
Identity = 281/636 (44.18%), Postives = 401/636 (63.05%), Query Frame = 2
Query:   38 MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPTKIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLSYIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNNAMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDAYISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYLPRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKDKNN-----AEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGFIVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQLIPRQITILPDITKKRCIPDSSQKHSPIDIQLSEIPHEECNLMKTEFICIDNLPKNMSKAALIFDINRPSDGCVIIPQRESSK-AFLYYPECNVDEWKAIITKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVDVKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGV--ASTFGLLDTVRKTYLRGKPIYADVIKLASINFET---PNKKRK 1912
            MNTD +YV NIPS+ KPVLLLHELENKW+RMLYLEN+FSG+RDA IYNP+     F  G+QKLR K+ D  K L+V YVWFPP NVF+TTMIP+GAS+LSYIFVENLHK+    E+  DLPLQ  D+K WDIPER DKC+IIKLP +SN AMRS++LGT+R+R I+G FP+  E+IIS+   LS  M P++I+  ++ ++ ++NF +LH+IPSKL VK L+++ K +NIKGCRVFF+ET N +K GF+Y+P++LP+ +F+ LFEKL + E+T   G  +RI + E WFQP  A+PP + D+        E VL +YL+L  IP N VA+QL +  P  +KL +F VN+LSTQIGF+V+RGLS     ++I HFRNL +GG  I VDE+++ C+NL  +   R  + IPR   +   I +   I   +  +   D  L     +   LMK+EFIC+ NLP +M+++A++F+I     GCV+    +    AF+Y P+C    W+ +I K + K F      +  ++ ++  S +T   +    +  IF YN        E   +   LI  +  ++T NG +        G         L+ +R   ++GKP++A+++  A +  +    P KK +
Sbjct:    1 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNL-ADIETRDAEYIPRPHPLESIIREALTIQLMALSNQLKDESLKLSCPKGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASKTFKA----ISWIFTRSTCSRMTIAWQLLAMSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVR 631          
The following BLAST results are available for this feature:
BLAST of SMED30007830 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007830 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000047325.10.000e+099.84SMESG000047325.1[more]
SMESG000070344.10.000e+046.43SMESG000070344.1[more]
SMESG000070604.10.000e+044.84SMESG000070604.1[more]
SMESG000070322.10.000e+044.84SMESG000070322.1[more]
SMESG000017548.10.000e+044.18SMESG000017548.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007830 ID=SMED30007830|Name=SMED30007830|organism=Schmidtea mediterranea sexual|type=transcript|length=1994bp
ATTTTTTTAATAAAAATATAAACATTTAAAACATTTTATGAATACTGATT
ATATTTATGTTTGCAATATACCAAGTAATTTTAAACCAGTTCTTCTACTC
CATGAATTGGAAAACAAATGGGCTCGTATGCTGTATTTAGAAAATACATT
TAGTGGCTTACGTGATGCTTTTATTTATAATCCAACTAAAATTTTCGATC
ATTTTCAACGTGGTATCCAAAAACTCCGTGACAAAATTATAGATGGAGTT
AAACCTTTAACTGTGTACTATGTCTGGTTTCCACCTGAAAACGTTTTTGT
AACAACAATGATTCCCGAAGGTGCTTCAATGCTTTCATATATATTTGTGG
AGAATTTACACAAAGATATAATTCCTCCCGAAATTATTTATGATTTACCT
TTGCAGGCGATTGATGTTAAAGCTTGGGATATTCCGGAGAGGCATGACAA
GTGCATGATTATCAAATTACCTGTCACTAGCAATAATGCAATGAGAAGTT
TTATATTGGGTACAATGAGAAGTAGAATGATACGTGGTGCATTTCCAAGA
GCATCAGAATTAATAATCAGTGAAGATTGTGTGTTAAGTAATAATATGAC
TCCACGTTCGATTAAAGTTAATCAAACGCAGGATGCTTATATTTCAAATT
TTTTTTATTTACATGACATACCGTCAAAATTAACAGTTAAAATGTTAATT
GAAGCTGCAAAGACCGAAAACATTAAAGGATGTCGAGTATTTTTTGTGGA
AACGCACAACAACATGAAATATGGATTTATGTACTTACCAAGGTCACTGC
CACATACGAGGTTTATGGCACTTTTTGAAAAATTATCGTCAATGGAATTC
ACGCTACCTAGTGGAGATCGTGCACGTATTAAAATTCAAGAGACGTGGTT
TCAGCCATGGCGAGCAGAGCCTCCGGTGGTGAAGGACAAAAATAACGCAG
AAATGGTGCTAACTGACTACTTGCATTTATCGGGAATACCCGCAAATATG
GTAGCAGCTCAATTGTCCATTAGTTGTCCATGCCCATTAAAGTTAAGCAT
TTTCCCAGTCAATGAACTATCCACACAAATTGGATTTATTGTTATCCGCG
GACTCAGTGAATATGAAGTTAATGTTGTAATTAATCACTTTAGAAATCTT
GAGCTTGGAGGCACTGCAATAAGTGTTGACGAAGTACACATTTGTTGTCA
TAATTTACCACAAAATTTCTCCGAGAGACCTTTTCAATTAATTCCCAGGC
AAATTACAATTCTTCCAGATATTACCAAAAAACGATGTATTCCAGATTCT
AGTCAAAAACATTCTCCTATTGACATACAACTTAGTGAAATTCCTCATGA
AGAATGTAATTTAATGAAAACCGAATTTATTTGTATTGATAATTTGCCAA
AAAATATGTCGAAGGCAGCTTTAATTTTCGATATAAATCGTCCCTCAGAT
GGATGTGTCATTATTCCTCAACGGGAATCCAGTAAAGCATTTTTATATTA
TCCAGAATGTAATGTCGACGAATGGAAGGCAATTATTACAAAGTTTTCTC
AGAAACAATTTTCCGGCAAAAAGGGAAATGTATATGAAGTTTATTTACAG
ACTTGTGATTCTATAATAACCAATAATCTGGAGCAATTCGTGAATACCAA
TATCATATTTGCATATAACATTTCTCGTGACGTAGACGTGAAAGAGATAT
TCGAAATTGATAAACCATTAATAGCATACGTAGAATTATTAGAAACGACA
AATGGTAGCAAAAGTGCGTTCATAATGACTAATGGAGGAGTTGCTTCAAC
TTTTGGATTACTCGACACTGTTAGAAAAACATACTTGCGTGGAAAGCCAA
TATACGCCGATGTAATAAAACTCGCTAGTATTAATTTCGAAACTCCCAAT
AAAAAGCGAAAAATGTACACCGACCAATGCTAATGTTTTACTATTAATAA
AAATGTATTGTGTTAATGAATAAAAATGTTTCATTGTTTTGTCA
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protein sequence of SMED30007830-orf-1

>SMED30007830-orf-1 ID=SMED30007830-orf-1|Name=SMED30007830-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=632bp
MNTDYIYVCNIPSNFKPVLLLHELENKWARMLYLENTFSGLRDAFIYNPT
KIFDHFQRGIQKLRDKIIDGVKPLTVYYVWFPPENVFVTTMIPEGASMLS
YIFVENLHKDIIPPEIIYDLPLQAIDVKAWDIPERHDKCMIIKLPVTSNN
AMRSFILGTMRSRMIRGAFPRASELIISEDCVLSNNMTPRSIKVNQTQDA
YISNFFYLHDIPSKLTVKMLIEAAKTENIKGCRVFFVETHNNMKYGFMYL
PRSLPHTRFMALFEKLSSMEFTLPSGDRARIKIQETWFQPWRAEPPVVKD
KNNAEMVLTDYLHLSGIPANMVAAQLSISCPCPLKLSIFPVNELSTQIGF
IVIRGLSEYEVNVVINHFRNLELGGTAISVDEVHICCHNLPQNFSERPFQ
LIPRQITILPDITKKRCIPDSSQKHSPIDIQLSEIPHEECNLMKTEFICI
DNLPKNMSKAALIFDINRPSDGCVIIPQRESSKAFLYYPECNVDEWKAII
TKFSQKQFSGKKGNVYEVYLQTCDSIITNNLEQFVNTNIIFAYNISRDVD
VKEIFEIDKPLIAYVELLETTNGSKSAFIMTNGGVASTFGLLDTVRKTYL
RGKPIYADVIKLASINFETPNKKRKMYTDQC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002109X1 cell
PLANA:0002111X2 cell