Ast6 protein

Overview
NameAst6 protein (CCW36844.1)
Smed IDSMED30007813
Length (bp)783
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ast6 protein (SMED30007813) t-SNE clustered cells

Violin plots show distribution of expression levels for Ast6 protein (SMED30007813) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ast6 protein (SMED30007813) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ast6 protein (SMED30007813) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007813

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30007813h1SMcG0009029 SmedASXL_009671SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30007813h1SMcG0009029 SmedASXL_009671SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30007813h1SMcG0009029 Contig41548newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007813h1SMcG0009029 Contig41548uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30007813h1SMcG0009029 OX_Smed_1.0.09523ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
neoblastSMED30007813h1SMcG0009029 SMED_00098_V2_1rGPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ast6 protein vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 85.8853 bits (211), Expect = 1.429e-18
Identity = 52/177 (29.38%), Postives = 90/177 (50.85%), Query Frame = 1 
Query:  130 LDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA 639
            L + DI   + F+   + + +WP       +V F  +  +    + ++ E L E ++    C++F +      +I +  +  GC+S +G +GG  Q ++L  P C+   +G V HE +H LGF H H R DRD +I + W+ IL      F+K +  ++  PY+ +SVMHY    F+
Sbjct:   73 LIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRDFISIIPM-YGCFSSVGRSGG-MQVVSLA-PTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFS 244
[Close]
BLAST of Ast6 protein vs. Ensembl Human
Match: MEP1A (meprin A subunit alpha [Source:HGNC Symbol;Acc:HGNC:7015])

HSP 1 Score: 77.7962 bits (190), Expect = 1.190e-15
Identity = 72/233 (30.90%), Postives = 102/233 (43.78%), Query Frame = 1 
Query:   88 LKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKD---LGNCVKFSE-STASHYIEVHSLNQGCYSLLGYTG-GPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEG---CDLDGPYETNSVMHYPSYGF---ACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC*T 753
            L  GLD  Q GD++L KS             N  R PN +  F   P    D  G+  +  +    +   L +CV F      S YI     + GC+S +G    G N  +     GC Y K  ++HE +HALGF H   R DRD+++ I WD+IL+ +   F   +     DL+ PY+  S+MHY  + F   A VP           +I      S  DL+ + + Y C T
Sbjct:   76 LAAGLDLFQ-GDILLQKS------------RNGLRDPNTRWTFPI-PYILADNLGLNAKGAILYAFEMFRLKSCVDFKPYEGESSYIIFQQFD-GCWSEVGDQHVGQNISIG---QGCAY-KAIIEHEILHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSFNKNASVPTITAKIPEFNSIIGQRLDFSAIDLERLNRMYNCTT 289
[Close]
BLAST of Ast6 protein vs. Ensembl Human
Match: MEP1A (meprin A subunit alpha [Source:HGNC Symbol;Acc:HGNC:7015])

HSP 1 Score: 77.411 bits (189), Expect = 1.634e-15
Identity = 72/233 (30.90%), Postives = 102/233 (43.78%), Query Frame = 1 
Query:   88 LKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKD---LGNCVKFSE-STASHYIEVHSLNQGCYSLLGYTG-GPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEG---CDLDGPYETNSVMHYPSYGF---ACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC*T 753
            L  GLD  Q GD++L KS             N  R PN +  F   P    D  G+  +  +    +   L +CV F      S YI     + GC+S +G    G N  +     GC Y K  ++HE +HALGF H   R DRD+++ I WD+IL+ +   F   +     DL+ PY+  S+MHY  + F   A VP           +I      S  DL+ + + Y C T
Sbjct:   48 LAAGLDLFQ-GDILLQKS------------RNGLRDPNTRWTFPI-PYILADNLGLNAKGAILYAFEMFRLKSCVDFKPYEGESSYIIFQQFD-GCWSEVGDQHVGQNISIG---QGCAY-KAIIEHEILHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSFNKNASVPTITAKIPEFNSIIGQRLDFSAIDLERLNRMYNCTT 261
[Close]
BLAST of Ast6 protein vs. Ensembl Human
Match: TLL2 (tolloid like 2 [Source:HGNC Symbol;Acc:HGNC:11844])

HSP 1 Score: 77.411 bits (189), Expect = 2.017e-15
Identity = 61/204 (29.90%), Postives = 90/204 (44.12%), Query Frame = 1 
Query:  166 KAHYSNTKR-WPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQ-GCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCD---LDGPYETNSVMHYPSYGFACVPGENVVFKR--DGGL---IDYNHVTSRNDLDMVRKFYAC 747
            +A  S T+R WP   + +     F G ++ I ++ +   +K    CV F E T      V S    GC S +G  GG  Q +++      +  G V HE  H +GF H H R DRD H+TI  + I       F+K E  +   L   Y+ +S+MHY    F+     + +  R  D G+   I      S+ D+   RK Y C
Sbjct:  149 RATTSRTERIWPGGVIPYVIGGNFTGSQRAIFKQAMRHWEKH--TCVTFIERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKF--GIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKC 348
[Close]
BLAST of Ast6 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 75.8702 bits (185), Expect = 4.981e-15
Identity = 65/227 (28.63%), Postives = 101/227 (44.49%), Query Frame = 1 
Query:   97 GLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSE-STASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCE---GCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVT------SRNDLDMVRKFYAC 747
            GLD  + GD+ LD++ I       +      RWP+  + +    +   + KG++       +  L  C+ F   +  ++YI V     GC+S +G      Q L++    C     TV+HEF+HALGF H   R DRD+++ I WDRIL+     F          L+ PY+  SVMHY    F       +V +    + D+  V       S +DL  + + Y C
Sbjct:   45 GLDLFE-GDIRLDRAQIR-----NSIIGEKYRWPHT-IPYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWAGETNYISVFK-GSGCWSSVGNRRVGKQELSI-GANCD-RIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTR----ISDFEDVIGQRMDFSDSDLLKLNQLYNC 255
[Close]
BLAST of Ast6 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 103.605 bits (257), Expect = 7.652e-26
Identity = 64/194 (32.99%), Postives = 101/194 (52.06%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH---YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCE---GCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVT-SRNDLDMVRKFYAC 747
            +RWPNN++ ++ +  +    + ++   + E       CVKF     S    Y+ +H  ++GCYSL+G TGG  QP++L + GC+   GT+ HE +HA+GF H   R+DRD++I + W  ++     QF K        LD PY+  S+MHY  Y F+    + +V K+ G       V  S  D+  + K Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSL-DSGCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast6 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 102.834 bits (255), Expect = 2.550e-25
Identity = 64/194 (32.99%), Postives = 101/194 (52.06%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH---YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCE---GCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVT-SRNDLDMVRKFYAC 747
            +RWPNN++ ++ +  +    + ++   + E       CVKF     S    Y+ +H  ++GCYSL+G TGG  QP++L + GC+   GT+ HE +HA+GF H   R+DRD++I + W  ++     QF K        LD PY+  S+MHY  Y F+    + +V K+ G       V  S  D+  + K Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSL-DSGCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast6 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 102.834 bits (255), Expect = 2.718e-25
Identity = 64/194 (32.99%), Postives = 101/194 (52.06%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH---YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCE---GCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVT-SRNDLDMVRKFYAC 747
            +RWPNN++ ++ +  +    + ++   + E       CVKF     S    Y+ +H  ++GCYSL+G TGG  QP++L + GC+   GT+ HE +HA+GF H   R+DRD++I + W  ++     QF K        LD PY+  S+MHY  Y F+    + +V K+ G       V  S  D+  + K Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSL-DSGCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast6 protein vs. Ensembl Celegans
Match: nas-14 (Zinc metalloproteinase nas-14 [Source:UniProtKB/Swiss-Prot;Acc:Q19269])

HSP 1 Score: 86.6557 bits (213), Expect = 3.424e-19
Identity = 58/193 (30.05%), Postives = 87/193 (45.08%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVH---SLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG---PYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            K WP  QV +        D++  + +   E +     CV+F   T   +  ++   ++  GC S +G  GG NQ ++L+   C +SKG + HE +HALGF H H R DRD+ + I  D I       F K     +D    PY+  SVMHY    F+      ++ K +   +   +  S  D   V K Y C
Sbjct:  123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYK--TKTCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQC 311
[Close]
BLAST of Ast6 protein vs. Ensembl Celegans
Match: nas-32 (Zinc metalloproteinase nas-32 [Source:UniProtKB/Swiss-Prot;Acc:O16977])

HSP 1 Score: 85.8853 bits (211), Expect = 8.420e-19
Identity = 68/224 (30.36%), Postives = 103/224 (45.98%), Query Frame = 1 
Query:  118 GDMVLDKSDIALINGFKAHYSNTKR---------WPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFS-ESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG--PYETNSVMHYPSYGFACVPGENVVFKRDGGLIDY--NHVTSRNDLDMVRKFYAC 747
            GD+ L+ + IA I+  ++  S  K+         WP   V + F        + IVR+ +  L+ +   C+KF   STA++ +++ S   GCYS  G  GG  Q L+L   GC  + GT  HE  H LG  HT MR DRD+++TI    +  S    F+K           YE  S MHY    F    G + +  +D  ++      + +  DL M+   Y+C
Sbjct:  178 GDINLNNNQIAKISSEQSSKSRRKKRQIDNLAQFWPGKVVYYYFDSGLTTTVQQIVRDAITFLESN--TCLKFELNSTATNRVKIFS-GVGCYSDTGMLGG-EQTLSL-GYGCEVT-GTAAHEIAHTLGLFHTQMRSDRDDYVTIDLTDVPESSQQNFIKLTEATSTNLVDYEYGSFMHYSGRAFVSSGGVDSIVPKDPVMVYTMGGRIVTFLDLKMLNTHYSC 395
[Close]
BLAST of Ast6 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 112.079 bits (279), Expect = 1.186e-28
Identity = 69/225 (30.67%), Postives = 109/225 (48.44%), Query Frame = 1 
Query:  100 LDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG-PYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRN-----DLDMVRKFYAC*T 753
            L S   GDM++ ++D+ + NG     S   RWPN  V +     F       +   + E  +    C++F   S+   YI +   N GC+S +G  GG  Q +NLQ+PGC+   GT  HE +HALGF+H   R +RD ++ I+++ + +S  + F K    +  G PY+  SVMHY    F+ + G+  +        D   +  RN     D+  + + Y C T
Sbjct:   89 LGSYLEGDMLVPQTDLIMKNGLPTQSS---RWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG-KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFS-INGQPTILAMQANGAD--KMGQRNGFSDYDIQKLNRMYDCGT 304
[Close]
BLAST of Ast6 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 112.079 bits (279), Expect = 1.186e-28
Identity = 69/225 (30.67%), Postives = 109/225 (48.44%), Query Frame = 1 
Query:  100 LDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG-PYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRN-----DLDMVRKFYAC*T 753
            L S   GDM++ ++D+ + NG     S   RWPN  V +     F       +   + E  +    C++F   S+   YI +   N GC+S +G  GG  Q +NLQ+PGC+   GT  HE +HALGF+H   R +RD ++ I+++ + +S  + F K    +  G PY+  SVMHY    F+ + G+  +        D   +  RN     D+  + + Y C T
Sbjct:   89 LGSYLEGDMLVPQTDLIMKNGLPTQSS---RWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG-KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFS-INGQPTILAMQANGAD--KMGQRNGFSDYDIQKLNRMYDCGT 304
[Close]
BLAST of Ast6 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0080785)

HSP 1 Score: 88.1965 bits (217), Expect = 1.927e-20
Identity = 60/197 (30.46%), Postives = 96/197 (48.73%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH--YIEVHSLNQGCYSLLGYTGGPNQPLNLQ----NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEG---CDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHV-TSRNDLDMVRKFYAC 747
            KRWP N V++  +  F    K  ++  +  L+  L  C++F E+T     Y+ V + + GCY+ +GY G P Q +NL+      GC +  GT+ HEF+HALGF H      RD+ I + ++ I+      F K       D +  Y+ +S +HY    F+ + GE+ +   D   +    V  S  D+D +   Y C
Sbjct:   66 KRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQGAP-QEMNLEIYPLGEGC-FRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFS-INGEDTIVPLDSSAVIGQRVGLSSKDIDKINIMYKC 257
[Close]
BLAST of Ast6 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0343719)

HSP 1 Score: 88.1965 bits (217), Expect = 1.927e-20
Identity = 60/197 (30.46%), Postives = 96/197 (48.73%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH--YIEVHSLNQGCYSLLGYTGGPNQPLNLQ----NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEG---CDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHV-TSRNDLDMVRKFYAC 747
            KRWP N V++  +  F    K  ++  +  L+  L  C++F E+T     Y+ V + + GCY+ +GY G P Q +NL+      GC +  GT+ HEF+HALGF H      RD+ I + ++ I+      F K       D +  Y+ +S +HY    F+ + GE+ +   D   +    V  S  D+D +   Y C
Sbjct:   66 KRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQGAP-QEMNLEIYPLGEGC-FRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFS-INGEDTIVPLDSSAVIGQRVGLSSKDIDKINIMYKC 257
[Close]
BLAST of Ast6 protein vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 85.8853 bits (211), Expect = 1.058e-19
Identity = 55/188 (29.26%), Postives = 88/188 (46.81%), Query Frame = 1 
Query:  193 WPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF--SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQF--VKCEGCDLDG-PYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFY 741
            W    +++S+A  F       +   + E+      CVKF  +E      + +     GC+S +GY G  +Q LNL   GCM S  T++HE +HALGF HTH    RD ++ I+ D I + H   F  ++  G    G  Y+ +S+MHY  + F+      +V  +    I      S  D+  +++ Y
Sbjct:   56 WKGRTLVYSYAGGFSSLDIASIESAMAEISSK--TCVKFRRTEYKREPQVVIQKEGSGCWSYVGYLGRADQTLNL-GSGCM-SNRTIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAFSKNGQSTIVPLKSHAKIGQATQMSPKDVQTLKRMY 239
[Close]
BLAST of Ast6 protein vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 100.138 bits (248), Expect = 3.170e-25
Identity = 59/166 (35.54%), Postives = 88/166 (53.01%), Query Frame = 1 
Query:  190 RWPNNQVLFSFAPAFPGDKKGIVRECLVE---LQKDLGNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRD 675
            RWP     F + P         +    +E   L      CVKF   T  ++++ +     GC+S LG TGG +Q ++LQ+PGCM+S G   HE +HALGF+H   R DRD +++I W+ I+ +    F K E  +L+  Y+ +SVMHY  Y F+   G  ++ K D
Sbjct:   28 RWPKAVDGFVYVPYIMSTLYDDMDRITIETGMLDISSSTCVKFVPRTHQANFLNIQP-RYGCWSYLGMTGG-SQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFSEDGGPTIIPKPD 190
[Close]
BLAST of Ast6 protein vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 101.293 bits (251), Expect = 5.244e-25
Identity = 59/166 (35.54%), Postives = 88/166 (53.01%), Query Frame = 1 
Query:  190 RWPNNQVLFSFAPAFPGDKKGIVRECLVE---LQKDLGNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRD 675
            RWP     F + P         +    +E   L      CVKF   T  ++++ +     GC+S LG TGG +Q ++LQ+PGCM+S G   HE +HALGF+H   R DRD +++I W+ I+ +    F K E  +L+  Y+ +SVMHY  Y F+   G  ++ K D
Sbjct:   86 RWPKAVDGFVYVPYIMSTLYDDMDRITIETGMLDISSSTCVKFVPRTHQANFLNIQP-RYGCWSYLGMTGG-SQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFSEDGGPTIIPKPD 248
[Close]
BLAST of Ast6 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 97.4413 bits (241), Expect = 1.218e-23
Identity = 61/186 (32.80%), Postives = 97/186 (52.15%), Query Frame = 1 
Query:  199 NNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVR--KFYAC 747
            N  + +S + A+       ++E +  +++D   CV+F   T    Y+++     GC+S LG  GG  Q ++LQ+P C  S  TV HE +HALGF+H   R DRD ++TI W  I       F K +  +LD PY+ +SVMH+  Y F+   GE  +  +    +        +DLD+++  K Y+C
Sbjct:   94 NVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQP-KTGCWSYLGARGG-RQTISLQSPDCTGSGVTV-HELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFS-EDGEPTIVPKRNWNVKIGQRLGPSDLDIMKINKLYSC 273
[Close]
BLAST of Ast6 protein vs. Ensembl Zebrafish
Match: he1.2 (hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-2100])

HSP 1 Score: 95.9005 bits (237), Expect = 3.646e-23
Identity = 71/222 (31.98%), Postives = 108/222 (48.65%), Query Frame = 1 
Query:  118 GDMVLDKSDIALINGFKAHYSNTKRWPNN--QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPG---------ENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            GD+VL K+  ALI   K+ +   K+  NN  +V +  +  F  + K ++   +         C++F   S  + Y+ + + + GCYS +G TGG  Q ++L   GC+YS G  +HE  HALGF H   R DRD ++ I W+ I       F+K +  + + PY+  S+MHY    FA  PG         ENV   +  GL       S+ D+  + K Y C
Sbjct:   56 GDVVLPKNRNALICEDKSCF--WKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENKD-GCYSAIGRTGG-KQVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGL-------SKIDILRINKLYGC 263
[Close]
BLAST of Ast6 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 93.9745 bits (232), Expect = 7.144e-23
Identity = 60/185 (32.43%), Postives = 96/185 (51.89%), Query Frame = 1 
Query:  199 NNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVR--KFYA 744
            N  + +S + A+       ++E +  +++D   CV+F   T    Y+++     GC+S LG  GG  Q ++LQ+P C  S  TV HE +HALGF+H   R DRD ++TI W  I       F K +  +LD PY+ +SVMH+  Y F+   GE  +  +    +        +DLD+++  K Y+
Sbjct:   35 NVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQP-KTGCWSYLGARGG-RQTISLQSPDCTGSGVTV-HELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFS-EDGEPTIVPKRNWNVKIGQRLGPSDLDIMKINKLYS 213
[Close]
BLAST of Ast6 protein vs. Ensembl Xenopus
Match: polr2l.2 (polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-5898446])

HSP 1 Score: 102.064 bits (253), Expect = 3.508e-24
Identity = 68/214 (31.78%), Postives = 110/214 (51.40%), Query Frame = 1 
Query:  130 LDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSE-STASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDM--VRKFYAC 747
            L + DIA+  G  A    +  WP +     +V +  +  +  ++K  +R+ L+E   +   CV F E ST   ++++ S + GC+S +G TGG  Q L+L + GC+ +KG ++HE  HALGF H   R DRD +I + W  I  S    F   +  +LD PY+ +SVMHY  Y F+   G+  +  +     +       + LD+  V++ Y C
Sbjct:   50 LWQGDIAIETGRSATKCPSCLWPKSADGTVRVPYRLSADYSDNEKSSIRDALLEF--NTLTCVHFVERSTEEDFLDIVS-DSGCWSSIGRTGGA-QTLSLMSSGCL-AKGIIQHEVDHALGFYHEQSRSDRDTYIDVLWQYICESDWGSFEMVDTDNLDLPYDYSSVMHYGWYAFSNTSGQPSLRPKPDPTANIGQRYGLSPLDVSKVKELYGC 258
[Close]
BLAST of Ast6 protein vs. Ensembl Xenopus
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:Xenbase;Acc:XB-GENE-1011058])

HSP 1 Score: 99.3673 bits (246), Expect = 3.338e-23
Identity = 67/218 (30.73%), Postives = 112/218 (51.38%), Query Frame = 1 
Query:  118 GDMVLDKSDIALINGFKAHYSNTKRWPNNQ-----VLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDM--VRKFYAC 747
            G+M L +    LIN  ++  S+   WP +      V + F+  +  D+  + +  + E +     CV+F   T    ++ + S N GC S++G  GG  Q + L + GCM S+G ++HE  HALGF H HMR DRD+++TI  + I+ S+ + F K +  ++   Y+ NSVMHY    F+  P ++ +  +    I        + LD+  ++K Y C
Sbjct:   49 GNMALTEGGDILINIGRSATSSDYLWPKSADGTVVVPYIFSYNYSADELTLFKTAMQEFETL--TCVRFVPKTIQRDFLNIVS-NGGCLSMVGRNGG-GQKVELASYGCM-SRGVIQHELNHALGFYHEHMRSDRDDYVTIITENIIPSYENYFSKRKTNNMGIIYDYNSVMHYSRNTFSISPDKSTIVPKPDPSIPIGQRDGLSILDILKIKKLYQC 261
[Close]
BLAST of Ast6 protein vs. Ensembl Xenopus
Match: ENSXETT00000022029.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496745])

HSP 1 Score: 94.7449 bits (234), Expect = 1.305e-21
Identity = 64/221 (28.96%), Postives = 110/221 (49.77%), Query Frame = 1 
Query:  109 MQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQ-----VLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKR--DGGLIDYNHVTSRNDLDMVRKFYAC 747
            M  GD+ + KS  AL+   K+       WP ++     V ++ +  +   ++ I+R  + E+   +  C++F + +  S Y+ +   + GC+S +G  GG  Q ++L   GC++  G ++HE +H+LGF H   R DRDN+I I WD I       F+K    +L  PY+  SVMHY  + FA   G+  +  +     +I      S  D++ + + Y C
Sbjct:    1 MIQGDIAVKKSRNALMCPGKSCL-----WPRSRKGLVLVPYTLSNNYNSTERDIIRAAMDEVT--VLTCIQFVTYNNESDYLRIRPYD-GCWSYIGRVGGA-QDVSLMKTGCLH-HGVIQHELLHSLGFQHEQCRSDRDNYININWDNISHDKERNFLKMNTQNLGSPYDYLSVMHYGKFAFATNSGKPTLEPKGNPSAMIGQRVGLSSLDVEKINRLYQC 211
[Close]
BLAST of Ast6 protein vs. Ensembl Xenopus
Match: tll1 (tolloid like 1 [Source:NCBI gene;Acc:493401])

HSP 1 Score: 93.5893 bits (231), Expect = 4.411e-21
Identity = 63/193 (32.64%), Postives = 100/193 (51.81%), Query Frame = 1 
Query:  193 WPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGE-NVVFKRDGGL-IDYNHVTSRNDLDMVRKFYAC 747
            WP        V ++ A  F   ++ +++  L EL     +C++F   T  + ++   S N GC+S +G TGG +Q +++   GCM S G ++HE +HALGF+H H R DRDN++ I +  I     S F K    +L   Y+ +SVMHY  + F   PG+  ++ K D  + I   +  S  D+  + K Y C
Sbjct:  203 WPRATSGLVNVPYTIASVFDDSEQELIQGALNELMT--LSCIRFKARTIETDFLSFQSGN-GCWSSVGKTGG-SQEVSVSKSGCM-SHGIIQHETLHALGFIHEHCRSDRDNYVDIIYKYISEGDRSSFTKVNSNNLGLQYDYSSVMHYGRFTFTNTPGQATIIPKPDLSVPIGQRYGVSSLDVAKLNKLYNC 390
[Close]
BLAST of Ast6 protein vs. Ensembl Xenopus
Match: tll1 (tolloid like 1 [Source:NCBI gene;Acc:493401])

HSP 1 Score: 93.2041 bits (230), Expect = 4.841e-21
Identity = 60/193 (31.09%), Postives = 97/193 (50.26%), Query Frame = 1 
Query:  193 WPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVR--KFYAC 747
            WP        V ++ A  F   ++ +++  L EL     +C++F   T  + ++   S N GC+S +G TGG +Q +++   GCM S G ++HE +HALGF+H H R DRDN++ I +  I     S F K    +L   Y+ +SVMHY  + F   PG+  +  +    +        + LD+ +  K Y C
Sbjct:  117 WPRATSGLVNVPYTIASVFDDSEQELIQGALNELMT--LSCIRFKARTIETDFLSFQSGN-GCWSSVGKTGG-SQEVSVSKSGCM-SHGIIQHETLHALGFIHEHCRSDRDNYVDIIYKYISEGDRSSFTKVNSNNLGLQYDYSSVMHYGRFTFTNTPGQATIIPKPDLSVPIGQRYGVSSLDVAKLNKLYNC 304
[Close]
BLAST of Ast6 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.5666 bits (192), Expect = 3.266e-16
Identity = 50/180 (27.78%), Postives = 88/180 (48.89%), Query Frame = 1 
Query:  130 LDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH----YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYS-KGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA 639
            L + DI   + F+       +WP       ++ F  +  +    + ++ +   E ++    C++F    A H    ++ +  +  GC+S +G +GG  Q ++L  P C+   +G V HE +H LGF H H R DRD +I + W+ IL      F+K    ++  PY+ +SVMHY  + F+
Sbjct:   73 LVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRF---VAYHGQRDFVSILPM-AGCFSGVGRSGG-MQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFS 244
[Close]
BLAST of Ast6 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.1814 bits (191), Expect = 4.174e-16
Identity = 50/180 (27.78%), Postives = 88/180 (48.89%), Query Frame = 1 
Query:  130 LDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH----YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYS-KGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA 639
            L + DI   + F+       +WP       ++ F  +  +    + ++ +   E ++    C++F    A H    ++ +  +  GC+S +G +GG  Q ++L  P C+   +G V HE +H LGF H H R DRD +I + W+ IL      F+K    ++  PY+ +SVMHY  + F+
Sbjct:   52 LVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRF---VAYHGQRDFVSILPM-AGCFSGVGRSGG-MQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFS 223
[Close]
BLAST of Ast6 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.1814 bits (191), Expect = 4.174e-16
Identity = 50/180 (27.78%), Postives = 88/180 (48.89%), Query Frame = 1 
Query:  130 LDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH----YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYS-KGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA 639
            L + DI   + F+       +WP       ++ F  +  +    + ++ +   E ++    C++F    A H    ++ +  +  GC+S +G +GG  Q ++L  P C+   +G V HE +H LGF H H R DRD +I + W+ IL      F+K    ++  PY+ +SVMHY  + F+
Sbjct:   52 LVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRF---VAYHGQRDFVSILPM-AGCFSGVGRSGG-MQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFS 223
[Close]
BLAST of Ast6 protein vs. Ensembl Mouse
Match: Tll2 (tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044])

HSP 1 Score: 77.411 bits (189), Expect = 1.308e-15
Identity = 61/204 (29.90%), Postives = 91/204 (44.61%), Query Frame = 1 
Query:  166 KAHYSNTKR-WPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQ-GCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCD---LDGPYETNSVMHYPSYGFA-CVPGENVVFKRDGG----LIDYNHVTSRNDLDMVRKFYAC 747
            +A  S T+R WP   + +     F G ++ I ++ +   +K    CV F E T      V S    GC S +G  GG  Q +++      +  G V HE  H +GF H H R DRD H+TI  + I       F+K E  +   L   Y+ +S+MHY    F+  V  + ++ +RD       I      S+ D+   RK Y C
Sbjct:  146 RATTSRTERIWPGGVIPYVIGGNFTGTQRAIFKQAMRHWEKH--TCVTFVERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKF--GIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRRDDNGVRPTIGQRVRLSQGDIAQARKLYKC 345
[Close]
BLAST of Ast6 protein vs. Ensembl Mouse
Match: Mep1b (meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964])

HSP 1 Score: 74.7146 bits (182), Expect = 9.506e-15
Identity = 58/184 (31.52%), Postives = 85/184 (46.20%), Query Frame = 1 
Query:   97 GLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSE-STASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQF---VKCEGCDLDGPYETNSVMHYPSYGF 636
            GLD  + GD+ L+       NG  +   + KRWP+  + +    +   + KG++       +  L  C+ F   S  ++YI V     GC+S +G      Q L++    C     TV+HEF+HALGF H   R DRD+++ I WDRI       F          L+ PY+  SVMHY    F
Sbjct:   47 GLDLFE-GDIKLEA------NGKNSIIGDHKRWPHT-IPYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWSGEANYISVFK-GSGCWSSVGNIHAGKQELSI-GTNCD-RIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAF 217
[Close]
BLAST of Ast6 protein vs. UniProt/SwissProt
Match: sp|P55112|NAS4_CAEEL (Zinc metalloproteinase nas-4 OS=Caenorhabditis elegans OX=6239 GN=nas-4 PE=2 SV=4)

HSP 1 Score: 103.605 bits (257), Expect = 9.734e-25
Identity = 64/194 (32.99%), Postives = 101/194 (52.06%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH---YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCE---GCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVT-SRNDLDMVRKFYAC 747
            +RWPNN++ ++ +  +    + ++   + E       CVKF     S    Y+ +H  ++GCYSL+G TGG  QP++L + GC+   GT+ HE +HA+GF H   R+DRD++I + W  ++     QF K        LD PY+  S+MHY  Y F+    + +V K+ G       V  S  D+  + K Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSL-DSGCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast6 protein vs. UniProt/SwissProt
Match: sp|P31581|HCE2_ORYLA (High choriolytic enzyme 2 OS=Oryzias latipes OX=8090 GN=hceb PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.351e-21
Identity = 58/160 (36.25%), Postives = 81/160 (50.62%), Query Frame = 1 
Query:  298 CVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFAC---------VPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            C++F   T  + +I V S N GCYS LG  GG  Q L+L   GCMYS G ++HE  HALGF H   R DRD+++ I W  I+ +    F K +  +L+ PY+ +S+MHY    F+          +P  NV   +  G+       SR D+      Y C
Sbjct:  129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGM-------SRWDITRSNVLYNC 278
[Close]
BLAST of Ast6 protein vs. UniProt/SwissProt
Match: sp|P31580|HCE1_ORYLA (High choriolytic enzyme 1 OS=Oryzias latipes OX=8090 GN=hcea PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 7.582e-21
Identity = 56/160 (35.00%), Postives = 82/160 (51.25%), Query Frame = 1 
Query:  298 CVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFAC---------VPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            C++F   T  + +I V S   GCYS LG  GG  Q L++   GCMYS G ++HE  HALGF H   R DRD+++ I W+ I+ +    F K +  +L+ PY+ +S+MHY    F+          +P  NV   +  G+       SR D+  +   Y C
Sbjct:  120 CIRFVRRTNEYDFISVVS-KTGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGM-------SRWDITRINVLYNC 269
[Close]
BLAST of Ast6 protein vs. UniProt/SwissProt
Match: sp|P31579|LCE_ORYLA (Low choriolytic enzyme OS=Oryzias latipes OX=8090 GN=lce PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.021e-20
Identity = 66/236 (27.97%), Postives = 105/236 (44.49%), Query Frame = 1 
Query:   82 KLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQ--------GCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVV-FKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            ++    ++ +  GD+VL K+  A+   F A   ++ RWP +     +V +  +  +  D+K  +R  + E          F+E T  H++  ++           GC S++GY G   Q + LQ  GC+     ++HE +HALGF H H R DRD H+ I W+ I+      F K +  +L  PY+  S+MHY    F     E +         I      S  D+  V K Y C
Sbjct:   51 RMNNNSMEELLEGDLVLPKTRNAM-KCFGA--PDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKE----------FAEKTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast6 protein vs. UniProt/SwissProt
Match: sp|P07584|ASTA_ASTAS (Astacin OS=Astacus astacus OX=6715 PE=1 SV=2)

HSP 1 Score: 90.1225 bits (222), Expect = 2.809e-20
Identity = 55/166 (33.13%), Postives = 89/166 (53.61%), Query Frame = 1 
Query:  193 WPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQF-VKCEGCDLDGPYETNSVMHYPSYGFACVPG--ENVVFKRDG 678
            W    + ++FA     D+  I+   + EL++    C++F   +T S Y+E+ +   GC+S +G   G  Q ++LQ  GC+Y  GT+ HE +HA+GF H H R DRDN++TI +  +  S  S F +      +   Y+  S+MHY  Y F+   G  E +V  ++G
Sbjct:   59 WSGGVIPYTFAGVSGADQSAIL-SGMQELEEK--TCIRFVPRTTESDYVEIFTSGSGCWSYVGRISGAQQ-VSLQANGCVY-HGTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNG 219
[Close]
BLAST of Ast6 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 520.39 bits (1339), Expect = 0.000e+0
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 1 
Query:    4 LRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +RSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast6 protein vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 390.963 bits (1003), Expect = 2.346e-135
Identity = 180/249 (72.29%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    4 LRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGG-PNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            ++ +F + L +A + YVSGQCGCPRQKLLKR +DSM+MGDM+LDKSDIALINGFK HY+NTK+WPNN V++SFA  FP DK  +VR+CL ELQ+DLGNCVKF+ES   HYIEV+S  +GCYSLLGY  G P QPLNL+NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITI W RI TS CSQF KC+GC+LDGPYET SVMHY S GFAC P E VV ++DGG I YNH T+ NDL+M+R FY C
Sbjct:    1 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249
[Close]
BLAST of Ast6 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 365.925 bits (938), Expect = 1.976e-125
Identity = 169/250 (67.60%), Postives = 197/250 (78.80%), Query Frame = 1 
Query:    4 LRSLFCLLLIVAAVSYVSGQCGCPRQ--KLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +  L  LLL    + Y +GQCGCPRQ  KL  RGLD+++MGDM+LD +DI +INGFK HY+N+K+WPNN V ++FA  FP DK   VR+ L ELQKDL NCVKF E    HYIEV+S  +GCYS+LG TGGP+QPLNL+NPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITIKWDRILTS CSQF KC+GC+ DGPYET SVMHY S GFAC P E VV K++GG I YNH T+ NDL M++ FY C
Sbjct:    1 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 250
[Close]
BLAST of Ast6 protein vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 1.966e-99
Identity = 135/244 (55.33%), Postives = 176/244 (72.13%), Query Frame = 1 
Query:   16 FCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            F +L+++   + V+ QCGC ++KL+ R  +++ +GDM+L K+DIA+INGFK H    + WP  +V F+FA  FP DK+ +V++CL ELQ DLGNCV+F EST   +I VH+ N+GCY+++G TG   Q LNLQNP CM SKGT+KHEFIHALGFMHTHMR DRDN I+I W  I +S C  FVKCEGC +DG YET SVMHY S  F C  G N + +++G LIDYNH T+ NDL M++  Y C
Sbjct:    8 FLILIVLVLANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 251
[Close]
BLAST of Ast6 protein vs. TrEMBL
Match: A0A060Q750 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast5 PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.149e-72
Identity = 116/245 (47.35%), Postives = 156/245 (63.67%), Query Frame = 1 
Query:   22 LLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTG--GPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVF-KRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            LL I+  V   +G+CGC + KL KR + S+++ DM+LD  D+  I GF+ +  N +RW +N + ++F P FP DKK IVRE + EL  DL  CVK+ EST+ ++I+V S   GC+S +G     G +Q LNL+NPGC+YSKGT+KHEFIHA GFMHT MR DRD  + I W+RI +  C  FV C+ C+    Y+TNSVMHYPS  F C PG+ +    +    I YNH T   D  M++  Y C
Sbjct:   10 LLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPC-PGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 251
[Close]
BLAST of Ast6 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 100.908 bits (250), Expect = 4.480e-25
Identity = 67/197 (34.01%), Postives = 99/197 (50.25%), Query Frame = 1 
Query:  193 WPNNQVLFSFAP-----AFPGDKKGIVRECLVELQKDLGNCVKFS-ESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFY 741
            WP N    ++ P      F  ++K ++   L   +K    C++F        YI+V SL +GCYS +G TGG  Q L+L++ GC+Y K  ++HEF+HALGF H   R DRD HI I ++ I       F K +  +L+ PY+  SVMHY    F+         +P +NV   R   +       S ND+  ++K Y
Sbjct:   81 WPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQSL-EGCYSFVGRTGG-RQTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEM-------SANDILRIKKLY 265
[Close]
BLAST of Ast6 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 100.908 bits (250), Expect = 6.113e-25
Identity = 67/197 (34.01%), Postives = 99/197 (50.25%), Query Frame = 1 
Query:  193 WPNNQVLFSFAP-----AFPGDKKGIVRECLVELQKDLGNCVKFS-ESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFY 741
            WP N    ++ P      F  ++K ++   L   +K    C++F        YI+V SL +GCYS +G TGG  Q L+L++ GC+Y K  ++HEF+HALGF H   R DRD HI I ++ I       F K +  +L+ PY+  SVMHY    F+         +P +NV   R   +       S ND+  ++K Y
Sbjct:   92 WPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQSL-EGCYSFVGRTGG-RQTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEM-------SANDILRIKKLY 276
[Close]
BLAST of Ast6 protein vs. Ensembl Cavefish
Match: npsn (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191946])

HSP 1 Score: 98.2117 bits (243), Expect = 6.502e-24
Identity = 65/197 (32.99%), Postives = 96/197 (48.73%), Query Frame = 1 
Query:  193 WPNNQVLFSFAP-----AFPGDKKGIVRECLVELQKDLGNCVKFS-ESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFY 741
            WP N    ++ P      F   +K ++   L   +K    C++F        YI+V SL +GCYS +G TGG  Q L+L   GC++ K  ++HE +HALGF H   R DRD HI I ++ I+      F K +  +L+ PY+  SVMHY    F+         +P +NV   R   +       S ND+  ++K Y
Sbjct:   92 WPRNSDGNTYIPYIISNQFRPKQKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQSL-EGCYSFVGRTGG-GQTLSLNRRGCLHKK-IIQHELLHALGFHHEQCRSDRDQHIRILYENIIPGEEHNFDKEDTNNLNTPYDYGSVMHYERDAFSRNGQPTMVPIPDQNVAVGRAEEM-------SANDILRIKKLY 276
[Close]
BLAST of Ast6 protein vs. Ensembl Cavefish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 96.2857 bits (238), Expect = 2.363e-23
Identity = 57/160 (35.62%), Postives = 82/160 (51.25%), Query Frame = 1 
Query:  295 NCVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +C++F   T    Y+ + S N GCYS +G  G   Q ++L   GC+Y K TV+HE +HALGF H   R DRDNHI + W+ I+ +    F K    +   PY+ NSVM Y    F+         +P  NV F +   +       S+ND+  ++K Y C
Sbjct:   98 SCIRFKRRTNERDYLSIESRN-GCYSYVGRRGNA-QTVSLARNGCIYHK-TVQHELLHALGFNHEQTRSDRDNHIRVAWENIVDTMKHNFNKIATLNQGTPYDYNSVMQYHKTAFSKNGKPTMIPIPNANVAFGQATQM-------SQNDITRLKKLYKC 247
[Close]
BLAST of Ast6 protein vs. Ensembl Cavefish
Match: ENSAMXT00000050470.1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103035835])

HSP 1 Score: 95.9005 bits (237), Expect = 2.873e-23
Identity = 57/159 (35.85%), Postives = 79/159 (49.69%), Query Frame = 1 
Query:  298 CVKFSE-STASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            C++F   S    YI + S   GCYS +G  G   Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + WD I+ S    F K    +   PY+ NSVM Y    F+         +P  NV F +   +       S+ND++ + + Y C
Sbjct:  110 CIRFKRRSNERDYISIES-RSGCYSYIGRLGNA-QTVSLDRNGCIY-HNTVQHELLHALGFNHEQTRSDRDNHIRVVWDNIIDSMKHNFNKIATLNQGTPYDYNSVMQYHRTAFSKNGQPTMVPIPNSNVSFGQASQM-------SQNDINRLNRLYKC 258
[Close]
BLAST of Ast6 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.7146 bits (182), Expect = 1.600e-15
Identity = 60/204 (29.41%), Postives = 91/204 (44.61%), Query Frame = 1 
Query:  166 KAHYSNTKR-WPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG---PYETNSVMHYPSYGFACVPGENVVFKR--DGGL---IDYNHVTSRNDLDMVRKFYAC 747
            +A  + ++R WP   + +     F G +K + R+ +   +K    CV F E T    YI       GC S +G  GG  Q +++      +  G V HE  H +GF H H R DRD+H+TI    I       F+K E  ++D     Y+ +S+MHY    F+     + +  R  D G+   I      S+ D+   RK Y C
Sbjct:   12 RAATARSERIWPGGVIPYIVGGNFTGSQKAMFRQAMRHWEKH--TCVTFIERTDEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFSRGIFLDTILPRPDDSGVRPAIGQRTRLSQGDIAQARKLYRC 211
[Close]
BLAST of Ast6 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 1.182e-8
Identity = 34/95 (35.79%), Postives = 46/95 (48.42%), Query Frame = 1 
Query:  361 CYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG-PYETNSVMHYPSYGFAC 642
            C+S +G  G   Q +++   GC    GTV HE  HA+G++H   R DRD  I++    +      QF      D  G PY+  SVMHY     AC
Sbjct:    1 CWSFVGMIGVGEQVVSIGR-GCE-GVGTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRAC 93
[Close]
BLAST of Ast6 protein vs. Ensembl Nematostella
Match: EDO34721 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ4])

HSP 1 Score: 98.5969 bits (244), Expect = 1.004e-24
Identity = 73/220 (33.18%), Postives = 103/220 (46.82%), Query Frame = 1 
Query:  109 MQMGDMVLDKSDIALINGF-----KAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLD---GPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFY 741
            M  GD+ LD S   +++       +A       WPN ++ + F  A     K   R  + E + +   C++F E T  + YI V S   GC+S +G +GG  Q L+L        KGT  HE +H LGF H   R+DRDN+ITI WD I +     F K E   +D    PY+  S+MHYP + F+      +V K     I      S+ DL  + K Y
Sbjct:    1 MVEGDIYLDSSTTRIMDQLMSPSREALVDPVSHWPNGRLPYKFG-AVSNSVKEAFRAAIDEYEAN--TCIRFVERTDETDYILVVS-EGGCWSPIGRSGG-QQKLSLGT--YCDRKGTAMHELMHTLGFFHEQSRRDRDNYITIHWDNIQSDKQFNFQKYEHGKVDTLGAPYDYGSIMHYPMFAFSSNGRATIVPKDPTASIGQRSGFSKVDLQQLNKLY 213
[Close]
BLAST of Ast6 protein vs. Ensembl Nematostella
Match: EDO37770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEU5])

HSP 1 Score: 90.8929 bits (224), Expect = 1.589e-21
Identity = 69/251 (27.49%), Postives = 105/251 (41.83%), Query Frame = 1 
Query:   22 LLLIVAAVSYVSG-----QCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGD-KKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLD---GPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +LL   A+S VS        G PR  L +  +         ++  D+    G   H    K+WPN  + +       G   +G+++  + E +     CV+F + T            GC+S +G      Q ++L   GC + KGT  HE  HALGF H   R DRDN++T+ WD I T     F K     ++    PY+  S+MHY +  F+      +  K+ G  I      S  D+  +   Y C
Sbjct:    5 VLLCFTAISLVSSASIDENAGNPRSDLFEGDMILTPDQQQAVNNGDLGASRGAPIH---DKKWPNAILPYEIRSDLKGTPAEGVIQNAMREWESK--TCVRFQKRTTQKNYVYFFKGDGCWSYVGMLDRGAQAISL-GSGC-WHKGTAAHEIGHALGFYHEQSRPDRDNYVTVHWDNIKTDSKHNFNKYSADRINTFGTPYDFGSLMHYGTNYFSVNGRATITPKKSGAKIGQRDYLSDLDVHQMNLRYGC 248
[Close]
BLAST of Ast6 protein vs. Ensembl Nematostella
Match: EDO30445 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0S0])

HSP 1 Score: 88.5817 bits (218), Expect = 6.068e-21
Identity = 69/215 (32.09%), Postives = 102/215 (47.44%), Query Frame = 1 
Query:  118 GDMVLDKSDIALIN-GFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDL--GNCVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRI---LTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFY 741
            GD+ LD+S  A++  G  A  S  + W N +V + F     G     VR   +   K+     CV+F        YI + S   GC+S +G +GG  Q L+L   GC + +GT  HE +HA+GF H   R DRD++ITI WD I   +  +  ++ + E   L   Y+  S+MHYP Y F     +  +  R+G  I      S  D+  +   Y
Sbjct:    5 GDIYLDRSTKAILGMGRDAMSSPARHWLNARVPYVF-----GSVSSEVRSVFLAAIKEYEAKTCVRFVPRRGERDYIYIVS-EGGCFSSIGRSGGL-QKLSL-GTGCEH-RGTAIHEMMHAIGFFHEQSRLDRDSYITIYWDNIKPGMEYNFRKYKRGEADTLGYAYDLKSIMHYPKYAFT-KNRQPTIIARNGANIGQRDSFSAIDIQQINALY 209
[Close]
BLAST of Ast6 protein vs. Ensembl Nematostella
Match: EDO42421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T1])

HSP 1 Score: 85.5001 bits (210), Expect = 2.761e-20
Identity = 59/191 (30.89%), Postives = 91/191 (47.64%), Query Frame = 1 
Query:  187 KRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQG-CYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDG---PYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            K WP   V++   P F    + + +        +   C++F + TA+    V+  ++G C S +G  GG  Q L+L + GC + KGT+ HE  HALGF H   R DRD+++TI ++ I     S F+K    ++D    PY+  S+MHY    F+      +V K+ G         S  D+  V   Y C
Sbjct:   10 KLWPGGVVVYELDPRFERSSRAMEKLTQAISDYEKYTCIRFRKKTANDNDYVYIKDRGGCRSHVGRKGG-KQYLSLSS-GC-WHKGTIIHELGHALGFFHEQTRPDRDDYVTILFENIRLGMASNFMKHSAGEVDSLGTPYDYGSIMHYGPKIFSANGKPTIVAKKPGVKFGQRDGLSPIDIKQVNLLYKC 197
[Close]
BLAST of Ast6 protein vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 85.5001 bits (210), Expect = 3.646e-20
Identity = 56/191 (29.32%), Postives = 88/191 (46.07%), Query Frame = 1 
Query:  184 TKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRI---LTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            T RWP   + ++   +        +R  + ++  D   C++F E T  +        +GC S++G TG   Q ++L   GC Y  GTV HE  HALGF H   R DRDN++TI W+ I   +  + + + +     L  PY+  SVMHY    F+      +  K+ G  +      S+ D+  +   Y C
Sbjct:   38 TGRWPEGVMAYTIDRSLSNRAMNAIRAGM-KMWTD-NTCIRFKERTNEYAYARFWRGRGCSSMVGRTG-RRQDISLAG-GCWY-PGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQQMNLMYKC 223
[Close]
BLAST of Ast6 protein vs. Ensembl Medaka
Match: mc6ast5 (astacin like metallo-protease [Source:NCBI gene;Acc:100049509])

HSP 1 Score: 114.005 bits (284), Expect = 6.341e-30
Identity = 70/194 (36.08%), Postives = 102/194 (52.58%), Query Frame = 1 
Query:  181 NTKRWPNNQVLFSFAPAFPGDKKGIVRECLVEL-QKDL--GNCVKFSEST-ASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGL-IDYNHVTSRNDLDMVRKFYAC 747
            NT  WP +   F + P         +    +E   +D+  G C+KF   T  +H++++     GC+S LG TGG +Q L+LQ PGCM+S G   HEF+HALGF+H   R DRD+++TI W  I+  H   F K    +L+ PY+ +SVMHY  Y F+   G  ++ + D  + I      S  DL  +   Y C
Sbjct:   89 NTCLWPKSVDGFVYVPYIVSPLYDDMDRITIETGMQDIASGTCIKFVPRTHEAHFLDIQP-RYGCWSFLGQTGG-SQTLSLQTPGCMWS-GIAAHEFMHALGFVHEQSRSDRDHYVTIVWKNIMPDHAHNFRKQATNNLNSPYDYSSVMHYGRYAFSGDGGPTIIPRPDPYIPIGQRDGPSALDLHKINVLYDC 279
[Close]
BLAST of Ast6 protein vs. Ensembl Medaka
Match: mc6ast4 (astacin like metallo-protease [Source:NCBI gene;Acc:100049317])

HSP 1 Score: 101.679 bits (252), Expect = 2.929e-25
Identity = 63/220 (28.64%), Postives = 112/220 (50.91%), Query Frame = 1 
Query:  118 GDMVLDKSDIALINGFKAH--YSNTKRWPNN-----QVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASH-YIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVR--KFYAC 747
            G   L + DIA+  G ++   ++ +  W  +      + ++ +P +      ++++ +  +++  G CV+F   T    Y+++     GC+S LG  GG  Q L+LQNP CM   G + HEF+HALGF+H   R DRDN+IT+ W  I       F K +   LD PY+  S+MH+  Y ++ + G+  +  ++   +     +S +  D ++  + Y C
Sbjct:   62 GTTTLREGDIAVSTGRRSKMCFARSCLWSKSVDGHVYIAYTISPDYTEADVKLIKKGMENIEE--GTCVRFVPQTHQRDYVDIQP-KSGCWSYLGARGG-RQTLSLQNPDCM-QVGVISHEFMHALGFVHEQSRFDRDNYITVMWPNIWRDRLRNFEKFKTDSLDLPYDYGSIMHFGMYAYS-MDGQPTLVAKNNRNMKLGQASSLSHTDKLKINRLYQC 275
[Close]
BLAST of Ast6 protein vs. Ensembl Medaka
Match: mc6ast2 (astacin like metallo-protease [Source:NCBI gene;Acc:100049316])

HSP 1 Score: 98.2117 bits (243), Expect = 4.086e-24
Identity = 56/161 (34.78%), Postives = 84/161 (52.17%), Query Frame = 1 
Query:  295 NCVKFS--ESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +C++F   ++    ++ + S N GCYS +G  GG  Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + W+ I+      F K +  +   PY+ NSVM Y  Y F+         +P  NV F +   +       S+ND+D + + Y C
Sbjct:  118 SCIRFRPYQNGDHEWLSIESRN-GCYSYVGRQGG-GQTVSLSRQGCLY-HSTVQHELLHALGFNHEQTRSDRDNHIKVHWENIIDDMEYNFYKIDTLNQGTPYDYNSVMQYERYAFSKNNLPTMEPIPDSNVSFGQATQM-------SKNDIDRLNRLYNC 268
[Close]
BLAST of Ast6 protein vs. Ensembl Medaka
Match: mc6ast2 (astacin like metallo-protease [Source:NCBI gene;Acc:100049316])

HSP 1 Score: 97.0561 bits (240), Expect = 1.885e-23
Identity = 56/161 (34.78%), Postives = 84/161 (52.17%), Query Frame = 1 
Query:  295 NCVKFS--ESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFA--------CVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +C++F   ++    ++ + S N GCYS +G  GG  Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + W+ I+      F K +  +   PY+ NSVM Y  Y F+         +P  NV F +   +       S+ND+D + + Y C
Sbjct:  141 SCIRFRPYQNGDHEWLSIESRN-GCYSYVGRQGG-GQTVSLSRQGCLY-HSTVQHELLHALGFNHEQTRSDRDNHIKVHWENIIDDMEYNFYKIDTLNQGTPYDYNSVMQYERYAFSKNNLPTMEPIPDSNVSFGQATQM-------SKNDIDRLNRLYNC 291
[Close]
BLAST of Ast6 protein vs. Ensembl Medaka
Match: mc6ast3 (astacin like metallo-protease [Source:NCBI gene;Acc:100049508])

HSP 1 Score: 95.1301 bits (235), Expect = 6.289e-23
Identity = 76/266 (28.57%), Postives = 116/266 (43.61%), Query Frame = 1 
Query:    4 LRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAH----YSNTKRWPNNQ-----VLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKF-SESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFAC--------VPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            L+    +LL V+  S+ + +     + L K   D +   D      DIA ++    +     S++  WP +      + +  A  F   +  ++   L    +   +C++F   S    Y+ + S N GCYS +G  G   Q L+L   GC+Y   TV+HE +HALGF H   R DRD HI + W+ I +     F K    +LD PY+ NSVM Y  Y F+          P  NV F     +       S ND+  + + Y C
Sbjct:    8 LKLSVAILLAVSTCSWATEKELSVSELLWKANKDVVHTEDEPFVVDDIAYVSKSDRNADQCTSSSCLWPKSADGRVYIPYVIANHFSSSELQVIERGLQSFSE--VSCIRFIPYSGQRDYVHIQSQN-GCYSYVGRIGY-GQTLSLDRQGCIY-HSTVQHELLHALGFNHEQCRSDRDQHIRVLWENIQSGLEYAFDKLNTLNLDTPYDYNSVMQYHRYAFSANNRPTMEPYPDPNVPFGTATQM-------SNNDITRLNRLYKC 261
[Close]
BLAST of Ast6 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 520.39 bits (1339), Expect = 0.000e+0
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 1 
Query:    4 LRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            +RSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast6 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 452.21 bits (1162), Expect = 9.949e-164
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 1 
Query:  109 MQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            MQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC
Sbjct:    1 MQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 213
[Close]
BLAST of Ast6 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 447.588 bits (1150), Expect = 7.526e-162
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 1 
Query:  115 MGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            MGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC
Sbjct:    1 MGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 211
[Close]
BLAST of Ast6 protein vs. Planmine SMEST
Match: SMESG000072793.1 (SMESG000072793.1)

HSP 1 Score: 416.772 bits (1070), Expect = 8.116e-150
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 1 
Query:  163 FKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            FKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC
Sbjct:   11 FKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 205
[Close]
BLAST of Ast6 protein vs. Planmine SMEST
Match: SMESG000072794.1 (SMESG000072794.1)

HSP 1 Score: 417.927 bits (1073), Expect = 1.007e-149
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 1 
Query:  163 FKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 747
            FKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC
Sbjct:   49 FKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 243

HSP 2 Score: 74.7146 bits (182), Expect = 1.081e-15
Identity = 33/39 (84.62%), Postives = 36/39 (92.31%), Query Frame = 3 
Query:   63 MRMSPSETAQKRPGQHADGRYGARQERHRAYQWVQSSLL 179
            MRMSPSETAQKRPGQHADGRYGARQERHRAYQW +  ++
Sbjct:    1 MRMSPSETAQKRPGQHADGRYGARQERHRAYQWGKGHIM 39
The following BLAST results are available for this feature:
BLAST of Ast6 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ASTL1.429e-1829.38astacin like metalloendopeptidase [Source:HGNC Sym... [more]
MEP1A1.190e-1530.90meprin A subunit alpha [Source:HGNC Symbol;Acc:HGN... [more]
MEP1A1.634e-1530.90meprin A subunit alpha [Source:HGNC Symbol;Acc:HGN... [more]
TLL22.017e-1529.90tolloid like 2 [Source:HGNC Symbol;Acc:HGNC:11844][more]
MEP1B4.981e-1528.63meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of Ast6 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-47.652e-2632.99Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-42.550e-2532.99Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-42.718e-2532.99Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-143.424e-1930.05Zinc metalloproteinase nas-14 [Source:UniProtKB/S... [more]
nas-328.420e-1930.36Zinc metalloproteinase nas-32 [Source:UniProtKB/S... [more]
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BLAST of Ast6 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG67631.186e-2830.67gene:FBgn0039069 transcript:FBtr0084378[more]
CG67631.186e-2830.67gene:FBgn0039069 transcript:FBtr0334497[more]
CG152551.927e-2030.46gene:FBgn0028950 transcript:FBtr0080785[more]
CG152551.927e-2030.46gene:FBgn0028950 transcript:FBtr0343719[more]
CG118651.058e-1929.26gene:FBgn0028947 transcript:FBtr0080753[more]
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BLAST of Ast6 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l13.170e-2535.54high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
hce2l15.244e-2535.54high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
hce2l21.218e-2332.80high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
he1.23.646e-2331.98hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;... [more]
hce2l27.144e-2332.43high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
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BLAST of Ast6 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
polr2l.23.508e-2431.78polymerase (RNA) II (DNA directed) polypeptide L, ... [more]
kpna43.338e-2330.73karyopherin alpha 4 (importin alpha 3) [Source:Xen... [more]
ENSXETT00000022029.11.305e-2128.96astacin-like metalloendopeptidase [Source:NCBI gen... [more]
tll14.411e-2132.64tolloid like 1 [Source:NCBI gene;Acc:493401][more]
tll14.841e-2131.09tolloid like 1 [Source:NCBI gene;Acc:493401][more]
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BLAST of Ast6 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Astl3.266e-1627.78astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl4.174e-1627.78astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl4.174e-1627.78astacin-like metalloendopeptidase (M12 family) [So... [more]
Tll21.308e-1529.90tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044][more]
Mep1b9.506e-1531.52meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964][more]
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BLAST of Ast6 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P55112|NAS4_CAEEL9.734e-2532.99Zinc metalloproteinase nas-4 OS=Caenorhabditis ele... [more]
sp|P31581|HCE2_ORYLA6.351e-2136.25High choriolytic enzyme 2 OS=Oryzias latipes OX=80... [more]
sp|P31580|HCE1_ORYLA7.582e-2135.00High choriolytic enzyme 1 OS=Oryzias latipes OX=80... [more]
sp|P31579|LCE_ORYLA2.021e-2027.97Low choriolytic enzyme OS=Oryzias latipes OX=8090 ... [more]
sp|P07584|ASTA_ASTAS2.809e-2033.13Astacin OS=Astacus astacus OX=6715 PE=1 SV=2[more]
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BLAST of Ast6 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A060Q7860.000e+099.60Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7852.346e-13572.29Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7141.976e-12567.60Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
I1ZIC31.966e-9955.33Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7502.149e-7247.35Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
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BLAST of Ast6 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
c6ast14.480e-2534.01low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast16.113e-2534.01low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
npsn6.502e-2432.99low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast32.363e-2335.63six-cysteine containing astacin protease 3 [Source... [more]
ENSAMXT00000050470.12.873e-2335.85low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
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BLAST of Ast6 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007253.11.600e-1529.41pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
ENSPMAT00000004913.11.182e-835.79pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
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BLAST of Ast6 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ast6 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO347211.004e-2433.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377701.589e-2127.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO304456.068e-2132.09Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424212.761e-2030.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414203.646e-2029.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ast6 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mc6ast56.341e-3036.08astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast42.929e-2528.64astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast24.086e-2434.78astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast21.885e-2334.78astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast36.289e-2328.57astacin like metallo-protease [Source:NCBI gene;Ac... [more]
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BLAST of Ast6 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072791.10.000e+099.60SMESG000072791.1[more]
SMESG000072791.19.949e-164100.00SMESG000072791.1[more]
SMESG000072791.17.526e-162100.00SMESG000072791.1[more]
SMESG000072793.18.116e-150100.00SMESG000072793.1[more]
SMESG000072794.11.007e-149100.00SMESG000072794.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30007813
GenBankCCW36844.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007813 ID=SMED30007813|Name=Ast6 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=783bp
GATCTGAGAAGTTTATTTTGCCTTTTGTTGATAGTTGCGGCCGTGAGCTA
CGTATCTGGCCAATGCGGATGTCCCCGTCAGAAACTGCTCAAAAGAGGCC
TGGACAGCATGCAGATGGGAGATATGGTGCTCGACAAGAGCGACATCGCG
CTTATCAATGGGTTCAAAGCTCATTACTCGAATACCAAAAGATGGCCAAA
TAATCAAGTGTTGTTTTCATTTGCTCCAGCTTTCCCCGGTGACAAAAAGG
GTATCGTACGAGAGTGCTTAGTTGAATTGCAAAAGGATCTGGGAAATTGT
GTCAAGTTCAGTGAATCTACAGCTAGCCATTATATTGAGGTGCACTCTCT
CAATCAAGGTTGTTATTCTCTTCTGGGTTACACCGGAGGACCAAACCAGC
CCTTGAACTTGCAGAATCCAGGATGTATGTATTCGAAAGGAACAGTAAAA
CACGAGTTCATTCATGCCTTGGGATTCATGCACACCCATATGAGAAAGGA
CAGAGACAACCACATCACTATCAAATGGGATAGAATCTTGACCAGTCACT
GCAGTCAGTTTGTCAAATGTGAAGGTTGCGATTTGGACGGACCGTACGAA
ACCAACAGTGTAATGCATTATCCAAGCTACGGATTCGCGTGCGTTCCCGG
AGAAAATGTCGTCTTTAAACGAGACGGAGGACTGATTGACTACAATCACG
TAACATCAAGGAACGATTTGGACATGGTCCGAAAATTTTACGCTTGTTAA
ACTTTGAACAATAAATAATTGTTTAAAAAAAAA
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protein sequence of SMED30007813-orf-1

>SMED30007813-orf-1 ID=SMED30007813-orf-1|Name=SMED30007813-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
DLRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIA
LINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNC
VKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVK
HEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYE
TNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC*
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Aliases
The feature 'Ast6 protein' has the following synonyms
Synonym
Ast6
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0008233peptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001506Peptidase M12APRINTSPR00480ASTACINcoord: 160..177
score: 41.76
coord: 196..211
score: 43.96
coord: 233..246
score: 26.31
coord: 141..159
score: 45.53
IPR001506Peptidase M12APFAMPF01400Astacincoord: 61..247
e-value: 9.9E-36
score: 123.2
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 59..201
e-value: 1.4E-20
score: 84.4
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 25..247
e-value: 9.3E-47
score: 161.4
NoneNo IPR availablePANTHERPTHR10127DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAININGcoord: 35..247
NoneNo IPR availablePROSITEPS51864ASTACINcoord: 54..247
score: 42.728
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.906
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 59..245