SMED30007769
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007769 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of SMED30007769 vs. TrEMBL
Match: A0A1I8IXF1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 1.177e-6 Identity = 47/191 (24.61%), Postives = 83/191 (43.46%), Query Frame = 1 Query: 247 KLYTKWKKDRKKQNQYFTDDL--FRAFYIEDLYSRRVDCFY-SLLMF----SNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKG-HLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWS-RFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGY 792 +L+ WK K + F L +RA Y +L+ R+ + SL S D + E + + V S GCG P V A + +R ++ ++ IG D W Y+ + G +F ++ I ++ +MSY+ + P WE ++ KF I+VLD + + ++Q+ G+ Sbjct: 66 RLFNTWKDAPDKLDVSFMYKLSGWRAVYTLNLFRSRIQTVWLSLRQLMSPKSGIADQLTEIFGRTQRVVSIGCGPGPCVAAMLIVLRECFGITQY-VKLIGIDQFIGWGPYVVSMNGVEAAGPSGVEFWHERVEEATIDKLPNANVAIMSYTDAAVHTPKFWETMRLKFAFILVLDQRKGALSRELQRQGF 255
BLAST of SMED30007769 vs. TrEMBL
Match: A0A267GR90 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000056g2 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 1.222e-6 Identity = 47/191 (24.61%), Postives = 83/191 (43.46%), Query Frame = 1 Query: 247 KLYTKWKKDRKKQNQYFTDDL--FRAFYIEDLYSRRVDCFY-SLLMF----SNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKG-HLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWS-RFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGY 792 +L+ WK K + F L +RA Y +L+ R+ + SL S D + E + + V S GCG P V A + +R ++ ++ IG D W Y+ + G +F ++ I ++ +MSY+ + P WE ++ KF I+VLD + + ++Q+ G+ Sbjct: 127 RLFNTWKDAPDKLDVSFMYKLSGWRAVYTLNLFRSRIQTVWLSLRQLMSPKSGIADQLTEIFGRTQRVVSIGCGPGPCVAAMLIVLRECFGITQY-VKLIGIDQFIGWGPYVVSMNGVEAAGPSGVEFWHERVEEATIDKLPNANVAIMSYTDAAVHTPKFWETMRLKFAFILVLDQRKGALSRELQRQGF 316
BLAST of SMED30007769 vs. TrEMBL
Match: A0A267G0K6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033796g1 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 1.222e-6 Identity = 47/191 (24.61%), Postives = 83/191 (43.46%), Query Frame = 1 Query: 247 KLYTKWKKDRKKQNQYFTDDL--FRAFYIEDLYSRRVDCFY-SLLMF----SNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKG-HLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWS-RFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGY 792 +L+ WK K + F L +RA Y +L+ R+ + SL S D + E + + V S GCG P V A + +R ++ ++ IG D W Y+ + G +F ++ I ++ +MSY+ + P WE ++ KF I+VLD + + ++Q+ G+ Sbjct: 130 RLFNTWKDAPDKLDVSFMYKLSGWRAVYTLNLFRSRIQTVWLSLRQLMSPKSGIADQLTEIFGRTQRVVSIGCGPGPCVAAMLIVLRECFGITQY-VKLIGIDQFIGWGPYVVSMNGVEAAGPSGVEFWHERVEEATIDKLPNANVAIMSYTDAAVHTPKFWETMRLKFAFILVLDQRKGALSRELQRQGF 319
BLAST of SMED30007769 vs. TrEMBL
Match: A0A267FKL1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009940g3 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 1.288e-6 Identity = 49/210 (23.33%), Postives = 93/210 (44.29%), Query Frame = 1 Query: 247 KLYTKWKKDRKKQNQYFTDDL--FRAFYIEDLYSRRVD-CFYSLLMF----SNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLD-NRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWS-RFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNESAKQFYY 849 KL+ WK K + F L +RA Y +L+ R+ ++SL S + E + + + + S GCG P + + + +R + +K ++ IG D W Y+ + + + +F ++ I + +MSY+ + P WE ++ KF I+VLD + + ++Q+ G+ +F + S + FYY Sbjct: 91 KLFNSWKNAPDKLDVSFMYKLSGWRAVYTLNLFRSRIQTVWFSLKQLLSSQSGIGPQLAEILNRTQRIVSIGCGPGPCIASILIALRDYVGFTKY-VKLIGIDQFIGWGPYVTSMNSVEEAGASSVEFWHERVEEATIDSLPNADVAIMSYTDAAIHTPKFWETMRMKFAFILVLDQRKGALSRELQRQGFG--HF-EYEFKGSKRVFYY 296
BLAST of SMED30007769 vs. TrEMBL
Match: A0A267G8H2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009940g1 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 1.288e-6 Identity = 49/210 (23.33%), Postives = 93/210 (44.29%), Query Frame = 1 Query: 247 KLYTKWKKDRKKQNQYFTDDL--FRAFYIEDLYSRRVD-CFYSLLMF----SNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLD-NRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWS-RFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNESAKQFYY 849 KL+ WK K + F L +RA Y +L+ R+ ++SL S + E + + + + S GCG P + + + +R + +K ++ IG D W Y+ + + + +F ++ I + +MSY+ + P WE ++ KF I+VLD + + ++Q+ G+ +F + S + FYY Sbjct: 90 KLFNSWKNAPDKLDVSFMYKLSGWRAVYTLNLFRSRIQTVWFSLKQLLSSQSGIGPQLAEILNRTQRIVSIGCGPGPCIASILIALRDYVGFTKY-VKLIGIDQFIGWGPYVTSMNSVEEAGASSVEFWHERVEEATIDSLPNADVAIMSYTDAAIHTPKFWETMRMKFAFILVLDQRKGALSRELQRQGFG--HF-EYEFKGSKRVFYY 295
BLAST of SMED30007769 vs. Planmine SMEST
Match: SMESG000033630.1 (SMESG000033630.1) HSP 1 Score: 302.753 bits (774), Expect = 1.072e-104 Identity = 141/158 (89.24%), Postives = 149/158 (94.30%), Query Frame = 1 Query: 388 NDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNESAKQFYYYFTQ 861 +++I EFI+ C+NV S GCGAAPEVLAYIKF+R L C++LNL FIGYDSIGEWDIYIDVLKGHLDNRVT DFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNESAKQFYYYFTQ Sbjct: 6 HEDIKEFIKTCENVASLGCGAAPEVLAYIKFLRCHLECTRLNLNFIGYDSIGEWDIYIDVLKGHLDNRVTLDFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNESAKQFYYYFTQ 163
BLAST of SMED30007769 vs. Planmine SMEST
Match: SMESG000033621.1 (SMESG000033621.1) HSP 1 Score: 235.343 bits (599), Expect = 4.566e-78 Identity = 117/165 (70.91%), Postives = 129/165 (78.18%), Query Frame = 1 Query: 373 MFSNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNE--SAKQFYYYFTQ 861 MFSNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIG WDIYID L+ HLDNRVTFDFK T+D +S M+Q G CLMSY HW F +IWE LK+KF+LIIVLDV + ++ GY ++F D N E S K FYYYF Q Sbjct: 1 MFSNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGGWDIYIDALRRHLDNRVTFDFKNETVDRYSVSRMTQRGVCLMSYPHWDGFENIWESLKSKFNLIIVLDVNVRRFHRTLRNSGYYFQFFND-NFEEFSSPKSFYYYFEQ 164
BLAST of SMED30007769 vs. Planmine SMEST
Match: SMESG000049126.1 (SMESG000049126.1) HSP 1 Score: 115.546 bits (288), Expect = 2.138e-30 Identity = 64/204 (31.37%), Postives = 105/204 (51.47%), Query Frame = 1 Query: 253 YTKWKKDRKKQ--NQYFTDDLFRAFYIEDLYSRRVDCFYSLLMFSNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCNESAKQFYYYFT 858 + W+ + K QY++D F Y++ L+ RV FY +L+ + + + + + ++TSFGCG PE++AY+ F+R QL K L F D I W ++DV D +T F+ + NY+ + G C+MSY F +IWE +K KFHLII+LD + ++ G+ K+F + +K + YY + Sbjct: 24 FRNWQDNPNKHVVTQYYSDFNFGLCYVDKLFRSRVQSFYQVLLDISCIEEVKRRMLEDLSITSFGCGPGPEIVAYVSFMRSQLDVKK-RLDFTAVDKIAYWKYFLDVTP-ECDRNITITFQNSILSENYLQRIDNQGICIMSYPEAVNFGNIWESMKKKFHLIIILDQSKSQLHESLRFAGFTIKFFQ----FKGSKSYAYYLS 221
BLAST of SMED30007769 vs. Planmine SMEST
Match: SMESG000049128.1 (SMESG000049128.1) HSP 1 Score: 113.235 bits (282), Expect = 7.573e-29 Identity = 65/205 (31.71%), Postives = 105/205 (51.22%), Query Frame = 1 Query: 262 WKKDRKKQ--NQYFTDDLFRAFYIEDLYSRRVDCFYSLLMFSNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCN---ESAKQFYYYFTQ 861 W+ K Y++D F Y++ LY RV+ FY +L N + +++ ++TSFGCG PE+ AYI F+R +L K L + D IG W +++V+ D+ +T F+ T+ +Y+ + G C+MSY F DIW+L+ KF LII+LD + + +++ G+ T CN K + YY T+ Sbjct: 27 WQNQPNKHLVTYYYSDYNFGLCYVQKLYGSRVESFYEVLFDITTNLEVELEMKRNLSITSFGCGPGPEIAAYISFLRSELYVEK-RLNVLAVDKIGYWRDFVNVIT-KCDSNITLAFQNATLSASYLLGIRDQGVCIMSYPEAVNFGDIWDLMIKKFRLIIILDQSKQQLHCSLRETGF-------TICNFTFRGQKSYAYYLTK 222
BLAST of SMED30007769 vs. Planmine SMEST
Match: SMESG000049128.1 (SMESG000049128.1) HSP 1 Score: 112.849 bits (281), Expect = 1.002e-28 Identity = 65/205 (31.71%), Postives = 105/205 (51.22%), Query Frame = 1 Query: 262 WKKDRKKQ--NQYFTDDLFRAFYIEDLYSRRVDCFYSLLMFSNFNDNILEFIRKCKNVTSFGCGAAPEVLAYIKFVRGQLRCSKLNLRFIGYDSIGEWDIYIDVLKGHLDNRVTFDFKKLTIDTNYISEMSQGGACLMSYSHWSRFPDIWELLKTKFHLIIVLDVKDKLMQSKMQKCGYNSKYFTDTNCN---ESAKQFYYYFTQ 861 W+ K Y++D F Y++ LY RV+ FY +L N + +++ ++TSFGCG PE+ AYI F+R +L K L + D IG W +++V+ D+ +T F+ T+ +Y+ + G C+MSY F DIW+L+ KF LII+LD + + +++ G+ T CN K + YY T+ Sbjct: 34 WQNQPNKHLVTYYYSDYNFGLCYVQKLYGSRVESFYEVLFDITTNLEVELEMKRNLSITSFGCGPGPEIAAYISFLRSELYVEK-RLNVLAVDKIGYWRDFVNVIT-KCDSNITLAFQNATLSASYLLGIRDQGVCIMSYPEAVNFGDIWDLMIKKFRLIIILDQSKQQLHCSLRETGF-------TICNFTFRGQKSYAYYLTK 229 The following BLAST results are available for this feature:
BLAST of SMED30007769 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30007769 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30007769 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30007769 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30007769 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30007769 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007769 ID=SMED30007769|Name=SMED30007769|organism=Schmidtea mediterranea sexual|type=transcript|length=903bpback to top protein sequence of SMED30007769-orf-1 >SMED30007769-orf-1 ID=SMED30007769-orf-1|Name=SMED30007769-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=234bp MDPAIDTVMATAKNTKCRFNSYNLSRTGKLYTKWKKDRKKQNQYFTDDLFback to top Annotated Terms
The following terms have been associated with this transcript:
|