SMED30007764

Overview
NameSMED30007764
Smed IDSMED30007764
Length (bp)1784
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007764 (SMED30007764) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007764 (SMED30007764) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007764 (SMED30007764) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007764 (SMED30007764) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007764

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30007764SMESG000036249.1 SmedASXL_009114SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30007764SMESG000036249.1 dd_Smed_v4_9326_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007764SMESG000036249.1 dd_Smed_v4_9326_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30007764SMESG000036249.1 dd_Smed_v6_9326_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30007764SMESG000036249.1 dd_Smed_v4_9326_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007764 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006168:3057..24382 -8593
Homology
[Close]
BLAST of SMED30007764 vs. TrEMBL
Match: A0A267E481 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006847g2 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.607e-40
Identity = 131/512 (25.59%), Postives = 230/512 (44.92%), Query Frame = 1 
Query:  241 ASQFSNEDENV---KTINNKPVLPPIFSARERNYNLSELVALL--------PQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDED----------------CTDLRSTERMRDSLIPI------DETELIRKINDDV-GFYIIPIQR---------IRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPI-NTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIR 1644
            A QFS+ D +    + +N    LPPI S+   +    E   LL        P    L +  + L + E+  ++   ++++  +      LK++Y  +A  D  LTGLCSF E  +A   NN+ ++  L R+++S+FI  S  +   +NYEK +SF+GA  +   +  +S A      ++  ++      Y+H +D                    +   R+     P       D   ++R++  +V G   +P +R         +RL+ QR D   RGTLS  QL+ + +   L + + T ++++   ++ GGGQ++W+ F+  +E+V P  +TGL +P S+ PLDY +  P P +SWPH+E        + +    ++P      +    +                 +          Q   T ++ A  EPWFN+F+K  D +Y +D  ++G L   ++ E+    N +L L  +   L  ALD +    +    NIN  L+RL +R
Sbjct:  139 AEQFSSGDASAPLSERLNPYQPLPPIGSSPRASGVAMETAQLLNGLYGRLPPYAGRLTSAPLVLGSNEEYVVMRELQSQLRYLE--TGKLKQVYTELADSDDELTGLCSFVELHNAFVRNNIFLADPLARLVASVFI--SPYEPPKVNYEKVLSFIGASLH-LGSNSLSEASYYQAQQQQQQQLNGGSGYYHQDDQLQQVFNQPAAPQTPQQQQQQRQSRLTPPQSPTQLSPDRDLPRVLRQLEAEVAGIVALPERRDQPPINFESLRLAFQRADRLLRGTLSRDQLKRLVWDFRLPISEQTLDHVVALCRHRGGGQYDWEQFLGFMERVIPTQSTGLAMPYSRRPLDYARHYPEPNASWPHAEP-------QAAAQTLQLPAASSSAQQPLYR---------------TPSPPPQQQQPQHQAMLTQRQAALAEPWFNRFMKLADALYRRDSQARGFLSESQMREVIERYNSELDLGIDRDALTKALDAARGGGNGGLTNINELLRRLGVR 623
[Close]
BLAST of SMED30007764 vs. TrEMBL
Match: V4ABX9 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_233043 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.984e-40
Identity = 134/449 (29.84%), Postives = 215/449 (47.88%), Query Frame = 1 
Query:  343 ELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAK-IDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSP-ARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGF--YIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMG-GGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEM-RGSNYDFRIPNIKHI-NKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTL----------KETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLS 1638
            EL+  LP+G+ LP + I L + E+  L+ +   E+    +    L+ +Y+ I K  D +++G C +K  V A     V++S D +R+++  FI   ++  + +NYEK +SF+G    +F     SP A   SP+    +   E P Y+     T +  TERM       D T+L+      +    Y I  +R+    Q +D   R TLS  Q++DIC    + L +S  + +L R + +   GQ+NW  FV  LE+VQP  TGL IP SK PL+Y KQ+P P + WP ++SP  H  + R   +  R  ++K + N +D  +                       +D   +    L          K+     PWF +F++  + +Y +DVN+ GILP  +++  + + N+   LN     +  AL  S   Q+ Q  NI+SFL RL 
Sbjct:  249 ELIRGLPRGEFLPERPIRLDDDEEWQLLKVIGEELEG--EDPMKLRHVYIHITKNGDKSMSGYCPYKYLVYALQDMKVNLSSDSIRIVAGQFIDPDKEPGR-VNYEKFLSFIGTALKNFGR---SPRAGRYSPI--DFKMIGEKPEYYESRPATPI--TERMEHIFNDRDVTKLLHMTEQQLKADEYEINFERLIADFQYHDREVRETLSYNQIKDICRDFRIPLTESLLQQILSRCQDLNKDGQYNWIKFVYFLERVQPSKTGLYIPRSKKPLEYAKQVPHPTTEWPKAKSPPPHTPLWRKEEHLKRSQDMKPLSNSYDREEPTQ---YQPQPPQERREVIPHAEIDRMDKELKELEKNYEVMKRNKKADSSSPWFKRFMELANALYGQDVNNYGILPYDDVWNWTRLHNEACNLNIPDYVVTKALRESA--QNGQ-VNIHSFLTRLG 681
[Close]
BLAST of SMED30007764 vs. TrEMBL
Match: A0A267G0A5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006847g4 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 5.855e-40
Identity = 131/513 (25.54%), Postives = 229/513 (44.64%), Query Frame = 1 
Query:  241 ASQFSNEDENV---KTINNKPVLPPIFSARERNYNLSELVALL--------PQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDED-----------------CTDLRSTERMRDSLIPI------DETELIRKINDDV-GFYIIPIQR---------IRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPI-NTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIR 1644
            A QFS+ D +    + +N    LPPI S+   +    E   LL        P    L +  + L + E+  ++   ++++  +      LK++Y  +A  D  LTGLCSF E  +A   NN+ ++  L R+++S+FI  S  +   +NYEK +SF+GA  +   +  +S A      ++  ++      Y+H +D                     +   R+     P       D   ++R++  +V G    P +R         +RL+ QR D   RGTLS  QL+ + +   L + + T ++++   ++ GGGQ++W+ F+  +E+V P  +TGL +P S+ PLDY +  P P +SWPH+E        + +    ++P      +    +                 +          Q   T ++ A  EPWFN+F+K  D +Y +D  ++G L   ++ E+    N +L L  +   L  ALD +    +    NIN  L+RL +R
Sbjct:  105 AEQFSSGDASAPLSERLNPYQSLPPIGSSPRASGVAMETAQLLNGLYGRLPPYAGRLTSAPLVLGSNEEYVVMRELQSQLRYLE--TGKLKQVYTELADSDDELTGLCSFVELHNAFVRNNIFLADPLARLVASVFI--SPYEPPKVNYEKVLSFIGASLH-LGSNSLSEASYYQAQQQQQQQLNGGSGYYHQDDQLQQVFNQPAAPQTPQQQQQQQRQSRLTPPQSPTQLSPDRDLPRVLRQLEAEVAGIVASPERRDQPPINFESLRLAFQRADRLLRGTLSRDQLKRLVWDFRLPISEQTLDHVVALCRHRGGGQYDWEQFLGFMERVIPTQSTGLAMPYSRRPLDYARHYPEPNASWPHAEP-------QAAAQTLQLPAASSSAQQPLYR---------------TPSPPPQQQQPQHQAMLTQRQAALAEPWFNRFMKLADALYRRDSQARGFLSESQVREVIERYNSELDLGIDRDALTKALDAARGGGNGGLTNINELLRRLGVR 590
[Close]
BLAST of SMED30007764 vs. TrEMBL
Match: A0A267GGC5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006847g3 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.171e-39
Identity = 129/512 (25.20%), Postives = 228/512 (44.53%), Query Frame = 1 
Query:  241 ASQFSNEDENV---KTINNKPVLPPIFSARERNYNLSELVALL--------PQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDED----------------CTDLRSTERMRDSLIPI------DETELIRKINDDVGFYIIPIQR----------IRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPI-NTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIR 1644
            A QFS+ D +    + +N    LPPI S+   +    E   LL        P    L +  + L + E+  ++   ++++  +      LK++Y  +A  D  LTGLCSF E  +A   NN+ ++  L R+++S+FI  S  +   +NYEK +SF+GA  +   +  +S A      ++  ++      Y+H +D                    +   R+     P       D   ++R++  +V   +   +R          +RL+ QR D   RGTLS  QL+ + +   L + + T ++++   ++ GGGQ++W+ F+  +E+V P  +TGL +P S+ PLDY +  P P +SWPH+E        + +    ++P      +    +                 +          Q   T ++ A  EPWFN+F+K  D +Y +D  ++G L   ++ E+    N +L L  +   L  ALD +    +    NIN  L+RL +R
Sbjct:  145 AEQFSSGDASAPLSERLNPYQPLPPIGSSPRASGVAMETAQLLNGLYGRLPPYAGRLTSAPLVLGSNEEYVVMRELQSQLRYLE--TGKLKQVYTELADSDDELTGLCSFVELHNAFVRNNIFLADPLARLVASVFI--SPYEPPKVNYEKVLSFIGASLH-LGSNSLSEASYYQAQQQQQQQLNGGSGYYHQDDQLQQVFNQPAAPQTPQQQQQQRQSRLTPPQSPTQLSPDRDLPRVLRQLEAEVAGIVASPERRDQPPINFESLRLAFQRADRLLRGTLSRDQLKRLVWDFRLPISEQTLDHVVALCRHRGGGQYDWEQFLGFMERVIPTQSTGLAMPYSRRPLDYARHYPEPNASWPHAEP-------QAAAQTLQLPAASSSAQQPLYR---------------TPSPPPQQQQPQHQAMLTQRQAALAEPWFNRFMKLADALYRRDSQARGFLSESQVREVIERYNSELDLGIDRDALTKALDAARGGGNGGLTNINELLRRLGVR 629
[Close]
BLAST of SMED30007764 vs. TrEMBL
Match: A0A433TLP7 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_009819 PE=4 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.766e-36
Identity = 127/433 (29.33%), Postives = 194/433 (44.80%), Query Frame = 1 
Query:  340 SELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAK-IDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPA--------------RNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGF--YIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGG-QFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSV 1584
            S L+A +P+G  LP+  + L   +++ L++I   E+   P     L+ +YL I   +D  L+G C +++        N     DLL+++++LF+   R+    +NYEK +SF+G    +  T K S +              R  SP+R  HR       +F+D                    E +L R I + +    Y I  +R+  S Q+ D   RGTLSA Q++ ICF   + +++S   N+LQR +      Q+ W  FV LLE+VQP  TGL IP SK PL+Y K  P PA SWP           +GSN     P+ K        ++  P P   + +        R    NY +  S       E PWF  F+ F D +Y +D   +G LP  ++ E + + N    L      + SAL+ S 
Sbjct:  195 SRLLAQIPRGVFLPDSPLVLTERDEMRLLSILAWELR--PADPVKLRDVYLDITNTVDKQLSGYCGYQDLFHTLSRMNFKFPADLLQIVAALFVSDHRNQRD-VNYEKFLSFVGVALKNKSTIKPSNSSNTGNQYYTSNSNQRKHSPIR--HRPGSP---FFNDGG------------------EAKLFRMIEEQLNENEYFIDFERLINSFQQEDRDVRGTLSADQIKSICFQHRIPIQESLIANMLQRCEDSNRDEQYKWFDFVRLLERVQPAKTGLTIPTSKRPLEYAKVQPAPAPSWP-----------KGSNT--YTPSWKMDPPVSPRREEVPDPNREIISRMDQEL--RDLEKNYEELKSKSPRRKEEAPWFKNFMAFADALYRQDERYEGSLPYSDVAEWTRMYNDSFQLEIPENVISSALNQST 586
[Close]
BLAST of SMED30007764 vs. Planmine SMEST
Match: SMESG000036249.1 (SMESG000036249.1)

HSP 1 Score: 1046.96 bits (2706), Expect = 0.000e+0
Identity = 523/535 (97.76%), Postives = 527/535 (98.50%), Query Frame = 1 
Query:   49 PFLSLIKDLEQNSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 1653
            P+ +    + +NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRIS DNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVN KGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK
Sbjct:    7 PWFNERCGISENSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRISADNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNRKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 541
[Close]
BLAST of SMED30007764 vs. Planmine SMEST
Match: SMESG000036249.1 (SMESG000036249.1)

HSP 1 Score: 1046.57 bits (2705), Expect = 0.000e+0
Identity = 522/524 (99.62%), Postives = 522/524 (99.62%), Query Frame = 1 
Query:   82 NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 1653
            NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRIS DNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVN KGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK
Sbjct:   26 NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRISADNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNRKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 549
[Close]
BLAST of SMED30007764 vs. Planmine SMEST
Match: SMESG000036249.1 (SMESG000036249.1)

HSP 1 Score: 1046.57 bits (2705), Expect = 0.000e+0
Identity = 522/524 (99.62%), Postives = 522/524 (99.62%), Query Frame = 1 
Query:   82 NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 1653
            NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRIS DNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVN KGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK
Sbjct:   27 NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRISADNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNRKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 550
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BLAST of SMED30007764 vs. Planmine SMEST
Match: SMESG000036249.1 (SMESG000036249.1)

HSP 1 Score: 1046.57 bits (2705), Expect = 0.000e+0
Identity = 523/535 (97.76%), Postives = 527/535 (98.50%), Query Frame = 1 
Query:   49 PFLSLIKDLEQNSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQXXXXXXXXXXXXGRISVDNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 1653
            P+ +    + +NSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRIS DNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVN KGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK
Sbjct:    6 PWFNERCGISENSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTKIVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERNYNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRGSNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRISADNYSQNTSTLKETAHEEPWFNKFLKFVDLIYIKDVNRKGILPTKELFELSTVLNQKLGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK 540
[Close]
BLAST of SMED30007764 vs. Planmine SMEST
Match: SMESG000076643.1 (SMESG000076643.1)

HSP 1 Score: 155.606 bits (392), Expect = 1.902e-40
Identity = 86/299 (28.76%), Postives = 164/299 (54.85%), Query Frame = 1 
Query:  337 LSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLKRIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSRDDVQFLNYEKPISFLGAIFNSFQTE--------KMSPARNQSPLRESHRETVEHPLYFHDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPI--QRIRLSCQRNDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSFVDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSES 1203
            L  L++ +P    LP+  I++   ++ +++     ++ +      NL+ +Y+ +   D +LTG  S+ + +       + I+  L+++I++ F+  SR +   +NYEK +SFLGA     +++        + S ++   P  +  + +    +Y+HD  C   R+ E   +     D+  ++R + + V     PI  + +RL+ QR+D   R TLS +Q++++C+  ++ ++ S  E ++ R K  GGGQ++WQ F+  LE+VQP+ TGL I  S+MP DY+K +P P+SSWP +E+
Sbjct:   92 LDNLLSTMPDFNRLPDIPINITPDQENAILLETNYQLANFD--ATNLRDLYMSLTNADPDLTGCISYNDLLFTLQRKQIPITEQLIQLIAAQFV--SRKESMKVNYEKILSFLGAALRLNKSDSNEDHFASQTSRSQKHGPHEQERQRSPGRGVYYHDSTC---RNEEENMNFYTEKDDPAILRLLENSVRHQFTPINLESLRLAFQRSDRLLRSTLSEQQIKELCYQHDVRIEDSVIERVIHRCKQRGGGQYDWQQFIRYLERVQPVQTGLDIRASRMPNDYIKYVPHPSSSWPQAET 383
The following BLAST results are available for this feature:
BLAST of SMED30007764 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E4811.607e-4025.59Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
V4ABX91.984e-4029.84Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A267G0A55.855e-4025.54Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GGC51.171e-3925.20Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A433TLP71.766e-3629.33Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
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BLAST of SMED30007764 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007764 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000036249.10.000e+097.76SMESG000036249.1[more]
SMESG000036249.10.000e+099.62SMESG000036249.1[more]
SMESG000036249.10.000e+099.62SMESG000036249.1[more]
SMESG000036249.10.000e+097.76SMESG000036249.1[more]
SMESG000076643.11.902e-4028.76SMESG000076643.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007764 ID=SMED30007764|Name=SMED30007764|organism=Schmidtea mediterranea sexual|type=transcript|length=1784bp
TATTAGCAACCTTGATTGCAAACAATTCAAATGCCTAAATTAGGGCGGCC
GTTTTTAAGTTTAATAAAGGATCTGGAACAGAATTCAAAAGACAAACGAC
TCTACTTTCTAAATGGGATCAGTGAGGTTCAGAGGAAAGCCCCATTTGGA
ACAAATTATACACCAGAGTTCTTGACAAAAATAGTAGGTTCTAAATATAG
AAAAGTTGAAGACGAAACTAGTGTTTCTGCTAGAAATTGCGCATCTCAAT
TTTCAAATGAAGATGAAAATGTCAAAACTATAAATAACAAGCCAGTTTTA
CCTCCTATCTTTTCCGCAAGAGAAAGAAATTATAATCTCTCTGAATTGGT
AGCGCTGTTGCCACAAGGGAAAGATTTACCTAATAAATCTATATCCCTCA
GAAACTATGAACAAATTTCACTAATTAACATAGCCAAGAATGAAATCTGT
GACATACCTGACTTTATTGCTAATCTCAAACGTATTTATTTAATGATAGC
TAAAATTGATATAAATTTGACCGGGTTGTGCAGCTTTAAAGAGTTTGTTG
ACGCTTGTTACCACAACAACGTTCACATTTCTTTCGACCTCCTCAGAGTG
ATCTCATCACTATTCATAGTTTCAAGCCGTGACGATGTGCAATTTCTGAA
TTATGAAAAGCCCATATCATTTTTGGGAGCAATTTTCAATAGCTTCCAAA
CTGAAAAAATGTCACCAGCTAGAAATCAGTCACCCTTGAGAGAAAGTCAT
CGCGAGACTGTCGAACATCCTCTTTATTTCCACGATGAAGATTGCACTGA
CCTCCGGTCAACTGAAAGAATGCGGGACTCATTAATTCCAATTGATGAAA
CTGAACTTATTAGGAAAATAAATGATGACGTTGGTTTTTATATTATTCCT
ATTCAAAGAATAAGGTTATCCTGTCAAAGAAACGATTCTTTTCATAGAGG
AACTTTGTCAGCAAAACAACTTGAAGACATTTGTTTTTCCGAAAATTTAA
ATCTAAAAAAATCTACGTTTGAAAATTTATTGCAAAGGAGCAAGTACATG
GGAGGAGGCCAATTTAACTGGCAGAGTTTTGTTGATTTACTTGAAAAAGT
GCAGCCGATTAATACCGGCCTTCAAATACCAAACAGCAAAATGCCATTAG
ATTATGTGAAGCAAATTCCTACCCCTGCATCAAGCTGGCCTCACTCTGAA
TCACCTGATATCCACCATGAAATGAGAGGAAGCAACTACGATTTTCGAAT
ACCGAATATAAAACATATAAACAAACATGATTTCAGTCAACAACATACAC
CAGCTCCTCAAACCTATGTCAATACAAACAATATAAATTACAATACTGGA
CGAATCTCGGTGGACAATTACTCGCAGAATACGAGCACTTTAAAAGAAAC
AGCACACGAAGAGCCTTGGTTTAACAAATTTCTTAAATTCGTAGACCTGA
TTTACATCAAAGACGTCAATAGTAAAGGAATTCTTCCGACAAAAGAATTA
TTTGAACTTTCAACAGTTTTGAATCAAAAATTAGGATTGAATTTCAACAC
TACTGAACTAATTTCTGCATTAGACGGATCAGTAGTTGATCAGGACAATC
AATTCTGTAATATTAATTCTTTCTTGAAACGACTTTCAATTCGATCATCC
AAATAAAATTTACATAATTTTTATATTTTCCTTTTTACCTCTAGTGCCAA
TATAAATGAAATTATATCTCTTTTATACATGTTAGACGTGATTTTGTTAC
TTTATCAATTTGTTTTAATTGCACTGAACTCGCT
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protein sequence of SMED30007764-orf-1

>SMED30007764-orf-1 ID=SMED30007764-orf-1|Name=SMED30007764-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=542bp
MPKLGRPFLSLIKDLEQNSKDKRLYFLNGISEVQRKAPFGTNYTPEFLTK
IVGSKYRKVEDETSVSARNCASQFSNEDENVKTINNKPVLPPIFSARERN
YNLSELVALLPQGKDLPNKSISLRNYEQISLINIAKNEICDIPDFIANLK
RIYLMIAKIDINLTGLCSFKEFVDACYHNNVHISFDLLRVISSLFIVSSR
DDVQFLNYEKPISFLGAIFNSFQTEKMSPARNQSPLRESHRETVEHPLYF
HDEDCTDLRSTERMRDSLIPIDETELIRKINDDVGFYIIPIQRIRLSCQR
NDSFHRGTLSAKQLEDICFSENLNLKKSTFENLLQRSKYMGGGQFNWQSF
VDLLEKVQPINTGLQIPNSKMPLDYVKQIPTPASSWPHSESPDIHHEMRG
SNYDFRIPNIKHINKHDFSQQHTPAPQTYVNTNNINYNTGRISVDNYSQN
TSTLKETAHEEPWFNKFLKFVDLIYIKDVNSKGILPTKELFELSTVLNQK
LGLNFNTTELISALDGSVVDQDNQFCNINSFLKRLSIRSSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR040774DUF5580
IPR011992EF-hand-dom_pair
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040774Protein of unknown function DUF5580PANTHERPTHR34830FAMILY NOT NAMEDcoord: 35..70
coord: 148..515
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 149..355