Very-long-chain enoyl-CoA reductase

Overview
NameVery-long-chain enoyl-CoA reductase
Smed IDSMED30007750
Length (bp)1101
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Very-long-chain enoyl-CoA reductase (SMED30007750) t-SNE clustered cells

Violin plots show distribution of expression levels for Very-long-chain enoyl-CoA reductase (SMED30007750) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Very-long-chain enoyl-CoA reductase (SMED30007750) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Very-long-chain enoyl-CoA reductase (SMED30007750) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007750

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30007750h1SMcG0017906 dd_Smed_v4_2085_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30007750h1SMcG0017906 dd_Smed_v4_2085_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30007750h1SMcG0017906 dd_Smed_v4_2085_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007750h1SMcG0017906 dd_Smed_v4_2085_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30007750h1SMcG0017906 dd_Smed_v4_2085_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007750h1SMcG0017906 dd_Smed_v4_2085_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007750h1SMcG0017906 Contig29884newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007750h1SMcG0017906 Contig29884uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Human
Match: TECR (trans-2,3-enoyl-CoA reductase [Source:HGNC Symbol;Acc:HGNC:4551])

HSP 1 Score: 305.449 bits (781), Expect = 3.063e-102
Identity = 160/307 (52.12%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  + TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVV--HLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Human
Match: TECR (trans-2,3-enoyl-CoA reductase [Source:HGNC Symbol;Acc:HGNC:4551])

HSP 1 Score: 305.834 bits (782), Expect = 8.453e-102
Identity = 160/307 (52.12%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  + TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:   43 EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVV--HLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 346          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Human
Match: TECRL (trans-2,3-enoyl-CoA reductase like [Source:HGNC Symbol;Acc:HGNC:27365])

HSP 1 Score: 216.468 bits (550), Expect = 9.913e-67
Identity = 123/305 (40.33%), Postives = 185/305 (60.66%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQ---KLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            E++I D +T K +     ++ ++TI D+K+ F     K+ PSR  L+      FL D   I  +    S V+LY  DLG Q+ W+TVFLAEY GPL IYLLFY +    Y+       L      LA +C   HY++ +LETLF+HK S    PL+NL+ +C++YWGF ++IAY+ NH LYTP  +G+ Q+    + F++ E GN  I+V+  +    G+     P P  NPF+++F  VSCPNYTYE+ SW+ ++VM Q+LP  +FT+    QM++WA  KH+ Y ++FN+Y   + ++IPF++
Sbjct:   61 EIEIFDAQTRKQICILDKVTQSSTIHDVKQKFHKACPKWYPSRVGLQLECGGPFLKDYITIQSI-AASSIVTLYATDLGQQVSWTTVFLAEYTGPLLIYLLFYLRIPCIYDGKESARRLRHPVVHLACFCHCIHYIRYLLETLFVHKVSAGHTPLKNLIMSCAFYWGFTSWIAYYINHPLYTPPSFGNRQITVSAINFLICEAGNHFINVMLSHPNHTGNNA-CFPSPNYNPFTWMFFLVSCPNYTYEIGSWISFTVMTQTLPVGIFTLLMSIQMSLWAQKKHKIYLRKFNSYIHRKSAMIPFIL 363          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Human
Match: TECRL (trans-2,3-enoyl-CoA reductase like [Source:HGNC Symbol;Acc:HGNC:27365])

HSP 1 Score: 183.341 bits (464), Expect = 2.332e-54
Identity = 109/266 (40.98%), Postives = 158/266 (59.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQ---KLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFL 932
            E++I D +T K +     ++ ++TI D+K+ F     K+ PSR  L+      FL D   I  +    S V+LY  DLG Q+ W+TVFLAEY GPL IYLLFY +    Y+       L      LA +C   HY++ +LETLF+HK S    PL+NL+ +C++YWGF ++IAY+ NH LYTP  +G+ Q+    + F++ E GN  I+V+  +    G+     P P  NPF+++F  VSCPNYTYE+ SW+ ++VM Q+LP  L
Sbjct:   61 EIEIFDAQTRKQICILDKVTQSSTIHDVKQKFHKACPKWYPSRVGLQLECGGPFLKDYITIQSI-AASSIVTLYATDLGQQVSWTTVFLAEYTGPLLIYLLFYLRIPCIYDGKESARRLRHPVVHLACFCHCIHYIRYLLETLFVHKVSAGHTPLKNLIMSCAFYWGFTSWIAYYINHPLYTPPSFGNRQITVSAINFLICEAGNHFINVMLSHPNHTGNNA-CFPSPNYNPFTWMFFLVSCPNYTYEIGSWISFTVMTQTLPGGL 324          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Human
Match: TECR (trans-2,3-enoyl-CoA reductase [Source:HGNC Symbol;Acc:HGNC:4551])

HSP 1 Score: 138.272 bits (347), Expect = 2.512e-39
Identity = 61/99 (61.62%), Postives = 76/99 (76.77%), Query Frame = 3
Query:  753 SIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:   52 SIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 150          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Celegans
Match: art-1 (Probable very-long-chain enoyl-CoA reductase art-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9N5Y2])

HSP 1 Score: 309.686 bits (792), Expect = 2.798e-104
Identity = 159/297 (53.54%), Postives = 206/297 (69.36%), Query Frame = 3
Query:  165 RTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVL--AQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            RT  ++ T   +S + TI  IKK  +  + K    RQ L+   + K L+DD+K+ DL     +  LY +DLGPQI W TVF+AEY GPLF+Y LFY +  + Y     +  +  A ++AF+ WS HY KR+ ET FIH+F +STMP  NL+KNCSYYWGFAAF+AYF NH L+TP  +GDLQ+Y GL  F++ E GNLSIH++  N+RP G+  R+IP P  NP S LF +VSCPNYTYEV SW+ +S+M QSLPA +FT AGF QM IWA GKHR Y KEF +YPK+RK+I+PFV+
Sbjct:   12 RTDNLIITLEGISGSETIKAIKKRIAQKKLKLTEERQALRVEPKGKPLADDQKLSDLGLSSQKAVLYVRDLGPQIAWKTVFMAEYAGPLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIHRFGNSTMPQFNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNLSIHILLRNLRPAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAIIFTTAGFAQMAIWAQGKHRNYLKEFPDYPKNRKAIVPFVL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Celegans
Match: F42F12.3 (pep chromosome:WBcel235:X:12350578:12351788:1 gene:WBGene00009635.1 transcript:F42F12.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F42F12.3)

HSP 1 Score: 73.1738 bits (178), Expect = 1.107e-14
Identity = 33/94 (35.11%), Postives = 56/94 (59.57%), Query Frame = 3
Query:  765 VFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFV 1046
            + M +R  G T  KIP        +L+ ++SCPNY  E++ W+GY+++  +LPA  F I  F  +   A+  H  YK++F  YP +R+++IP++
Sbjct:  155 ILMTLRKPGETGYKIPT------GFLYEYISCPNYFGEIMEWIGYAILGWNLPALAFAIFTFANIGPRAIAHHNWYKEKFPKYPPTRRALIPYI 242          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Celegans
Match: F19H6.4 (pep chromosome:WBcel235:X:12365688:12367255:1 gene:WBGene00008959.1 transcript:F19H6.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F19H6.4)

HSP 1 Score: 68.5514 bits (166), Expect = 4.760e-13
Identity = 32/93 (34.41%), Postives = 51/93 (54.84%), Query Frame = 3
Query:  762 VVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIP 1040
             +   +R  G T  KIP        +L+ ++SCPNY  E++ W+GYS++  +LPA  F I     +   A+  H+ YK+ F  YP +R+ +IP
Sbjct:  154 TILRELRADGGTGYKIPT------GFLYEYISCPNYAGEIMEWIGYSILAWNLPALAFAIFTIANIGPRAVAHHKWYKETFPGYPPNRRILIP 240          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Celegans
Match: ZK1251.3 (pep chromosome:WBcel235:IV:9683617:9684473:1 gene:WBGene00014241.1 transcript:ZK1251.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK1251.3)

HSP 1 Score: 53.9138 bits (128), Expect = 4.116e-8
Identity = 26/74 (35.14%), Postives = 41/74 (55.41%), Query Frame = 3
Query:  828 PFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFN-NYPKSRKSIIPFV 1046
            P  + F ++SCPNY  E L W+GY++  +S PA  F       +   A+  H+ Y+K F   YP+ R +++P V
Sbjct:  179 PRGHYFEYISCPNYFGECLQWIGYALAARSFPAIAFAFFIVCNLAPRAMSHHKWYQKMFGEQYPRDRHALVPGV 252          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Fly
Match: Sc2 (gene:FBgn0035471 transcript:FBtr0073159)

HSP 1 Score: 270.011 bits (689), Expect = 9.640e-89
Identity = 147/305 (48.20%), Postives = 194/305 (63.61%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLF-STDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDG-NYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            ME++I + + SK  G     S  T IGD++ L   T ++    +RQ L+   + K L D   +  L    S   +Y KDLGPQIGW TVFLAEY GPL +YL+FYF+    Y    +    L   +A  C++ HYVKR+LET+F+H+FSH+TMPLRNL KNC+YYWGF A+++Y  NH  +T        ++G L  F L ELGN S+H+   N+RP G+ VRKIP    NP + LF  VSCPNYTYE+ +W+ +SV+   L A+LF  AG  QMTIWAL KHR YKKEF +YP+ R+SI PFV+
Sbjct:    1 MELEILNAKNSKPYGKVKVPSGATPIGDLRALIHKTLKQTPHANRQSLRLELKGKSLKDTDTLESLSLR-SGDKIYVKDLGPQIGWKTVFLAEYAGPLIVYLIFYFRPELVYGKAASLPISLTTHIAAGCYTVHYVKRLLETIFVHRFSHATMPLRNLFKNCTYYWGFTAYVSYHVNHPQFTSPC--MCTVWGALGAFALCELGNFSVHIALRNLRPPGTKVRKIPVADGNPLTKLFDLVSCPNYTYEIGAWVSFSVLTSCLAAYLFAFAGAFQMTIWALAKHRNYKKEFKDYPRQRRSIFPFVL 302          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Zebrafish
Match: tecrb (trans-2,3-enoyl-CoA reductase b [Source:ZFIN;Acc:ZDB-GENE-030131-5154])

HSP 1 Score: 301.212 bits (770), Expect = 1.079e-100
Identity = 155/307 (50.49%), Postives = 208/307 (67.75%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T   L     +  N TIG+IK +F     ++ P+RQ ++   + K L D+  +  L   G+  + YF+DLG QI W TVFL EY GPL IYL+FYF+  + Y        + H+V+   LA  C S HYVKR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP +YG+ Q+   L +F+  ++GN SIH+   N+RP GS  RKIPYPT NPF+++F  VSCPNYTYE+ SWLG+++M Q LP   FT+ GF QMT+WA GKHR+Y KEF +YP  R  I+PF++
Sbjct:    5 EVEILDAKTKDKLCFLDKVEPNATIGEIKSMFHKSHPQWYPARQSIRLDPKGKSLKDEDVLQHLPV-GTTATFYFRDLGAQISWVTVFLTEYAGPLLIYLMFYFRVPFIYAPKYDFTTSKHWVV--HLACMCHSFHYVKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPIYGEQQIRLALTVFLFCQIGNFSIHIALRNLRPPGSKTRKIPYPTKNPFTWIFLLVSCPNYTYELGSWLGFTLMTQCLPVAFFTLVGFIQMTVWAKGKHRSYLKEFRDYPTLRSPILPFIL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Zebrafish
Match: tecra (trans-2,3-enoyl-CoA reductase a [Source:ZFIN;Acc:ZDB-GENE-090930-1])

HSP 1 Score: 270.011 bits (689), Expect = 5.430e-88
Identity = 145/305 (47.54%), Postives = 194/305 (63.61%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN---DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I DV+T + L     +    TI DIK +F      + P+RQ L    + K L D+  + +L   G+  + YF+DLG QI W TVFL E  GPL +YLLFYF+    Y    D      L   +A  C + HY KRI+ETLF+H+FSH TMP RN+ KNC Y+W FAA++AY+ NH LYT   YG+ Q+   L +F+  +LG+ SIH+   +++  GS  +KIPYPT NPF+++F  VSCPNYTYEV SWLG+++M Q +P   FT+ GF QMT+WA GKHR+Y KEF  YP  R  I+PFV+
Sbjct:   43 EVEILDVKTREKLLLLDKVEPTATILDIKSMFHKSNPHWYPARQSLCLDPKGKPLKDEDILQELPV-GTTATFYFRDLGAQIAWGTVFLIECIGPLVLYLLFYFRLPLIYAPKYDFTASKHLVVHIACACHTFHYAKRIMETLFVHRFSHGTMPFRNIFKNCIYHWCFAAWMAYYINHPLYTTPYYGEKQVKTALGIFLFCQLGSFSIHMALRSLKLPGSKTKKIPYPTKNPFTWIFALVSCPNYTYEVGSWLGFTLMTQCVPVLFFTVVGFIQMTVWAKGKHRSYLKEFKEYPTLRSPILPFVL 346          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Zebrafish
Match: tecrl2b (trans-2,3-enoyl-CoA reductase-like 2b [Source:ZFIN;Acc:ZDB-GENE-041010-116])

HSP 1 Score: 265.774 bits (678), Expect = 2.340e-86
Identity = 150/304 (49.34%), Postives = 201/304 (66.12%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQ----KLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPF 1043
            EV+I D ++   L     +  + TI +IK LF     K  PSRQ LK   E K LSD+  + DL   G+  +++F+DLGPQIGW+ VFLAE  GPLF+YLLFYF+  + Y    Y Y+ +     +LA +C   HY+K+++ET+FIH+FS  T+PLR ++  C YYWGFA++ AY+ NH LYTPA YG LQ+Y  L LF+L E GNLS+H++   +   GS   +IPYPT NPF++LF FVSCPNYTYEV SW+ ++VM Q +P  +F    F QMTIWA  KH+ Y KEF +YP+ R +IIP 
Sbjct:   46 EVEILDPKSKAQLCYLDKVEPSATIAEIKGLFHKTYPKLYPSRQALKLHPEGKALSDEDVLEDLPV-GTTATMFFQDLGPQIGWTMVFLAECLGPLFVYLLFYFRLPYIYRH-EYDYIRSPLKVVRLACWCHCLHYIKKLVETIFIHRFSDGTVPLRTIMLLCLYYWGFASWQAYYINHPLYTPATYGRLQIYSALALFLLCEFGNLSVHLILNRISCNGSKPTEIPYPTNNPFTWLFFFVSCPNYTYEVGSWISFAVMTQCVPVGVFAFLSFIQMTIWARAKHKTYVKEFKDYPELRTAIIPL 347          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Zebrafish
Match: tecrl2a (trans-2,3-enoyl-CoA reductase-like 2a [Source:ZFIN;Acc:ZDB-GENE-131121-138])

HSP 1 Score: 192.971 bits (489), Expect = 2.521e-60
Identity = 99/176 (56.25%), Postives = 132/176 (75.00%), Query Frame = 3
Query:  522 CWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            C S HY+KR+ ET+F+H+FSH TMPLR ++KNC YYWGFAA++AY+ NH LYTP  YG++Q+   L++F+L E GN SIH    +++  GS  R+ P+P+ NPF++LF FVSCPNYTYEV +W+G S+M Q +P  LFT  GF QMTIWA GKH+ Y +EF NYP  R  I+PF++
Sbjct:    1 CHSFHYIKRLFETIFVHRFSHGTMPLRAIVKNCVYYWGFAAWLAYYINHPLYTPPSYGEIQVNYALIIFVLCEAGNFSIHWSLNSLKCEGSKCRRFPHPSKNPFTWLFFFVSCPNYTYEVGAWVGLSIMTQCVPVALFTFVGFIQMTIWAKGKHQIYVREFKNYPNLRMPILPFIL 176          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Zebrafish
Match: tecrl2a (trans-2,3-enoyl-CoA reductase-like 2a [Source:ZFIN;Acc:ZDB-GENE-131121-138])

HSP 1 Score: 191.43 bits (485), Expect = 1.861e-59
Identity = 105/194 (54.12%), Postives = 142/194 (73.20%), Query Frame = 3
Query:  360 LYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPA 926
            +YF+DLGPQ+GW+ VFLAEY GPL IYLLFY +  + YN       + H V++  LA  C S HY+KR+ ET+F+H+FSH TMPLR ++KNC YYWGFAA++AY+ NH LYTP  YG++Q+   L++F+L E GN SIH    +++  GS  R+ P+P+ NPF++LF FVSCPNYTYEV +W+G S+M Q +P 
Sbjct:    1 MYFRDLGPQLGWTMVFLAEYIGPLLIYLLFYVRVPYIYNHKYTFTSSTHPVVS--LACACHSFHYIKRLFETIFVHRFSHGTMPLRAIVKNCVYYWGFAAWLAYYINHPLYTPPSYGEIQVNYALIIFVLCEAGNFSIHWSLNSLKCEGSKCRRFPHPSKNPFTWLFFFVSCPNYTYEVGAWVGLSIMTQCVPV 192          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Xenopus
Match: tecr (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:493561])

HSP 1 Score: 303.523 bits (776), Expect = 3.019e-101
Identity = 155/306 (50.65%), Postives = 210/306 (68.63%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQK----LAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  + TI DIK +F+    ++ P+RQ L+   + + L D+  + +L   G+  +LYF+DLG QI W TVFL EY GPL IYLLFYF+  + Y    Y ++ ++     LA  C S HY+KR+LETLF+H+FSH TMP RN+ KNC YYWGFAA++AY+ NH LYTP  YG+ Q+   +++F+  +LGN SIH+   N+RP GS  RKIP+PT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LFT+ GF QMTIWA GKHR+Y KEF +YP  R  I+PF++
Sbjct:   19 EVEILDAKTREKLCFLDKVEPHATIADIKNMFTKSHPQWYPARQSLRLDPKGRSLKDEDILQNLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLVIYLLFYFRVPFIYGP-KYDFITSRHSVVHLACICHSFHYIKRLLETLFVHRFSHGTMPFRNIFKNCMYYWGFAAWMAYYINHPLYTPPTYGEDQVKLAVIIFLFCQLGNFSIHIALRNLRPAGSKTRKIPFPTRNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFTLVGFIQMTIWAKGKHRSYLKEFRDYPPLRSPIVPFIL 322          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Xenopus
Match: tecr (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:493561])

HSP 1 Score: 304.294 bits (778), Expect = 3.396e-101
Identity = 155/306 (50.65%), Postives = 210/306 (68.63%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQK----LAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  + TI DIK +F+    ++ P+RQ L+   + + L D+  + +L   G+  +LYF+DLG QI W TVFL EY GPL IYLLFYF+  + Y    Y ++ ++     LA  C S HY+KR+LETLF+H+FSH TMP RN+ KNC YYWGFAA++AY+ NH LYTP  YG+ Q+   +++F+  +LGN SIH+   N+RP GS  RKIP+PT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LFT+ GF QMTIWA GKHR+Y KEF +YP  R  I+PF++
Sbjct:   43 EVEILDAKTREKLCFLDKVEPHATIADIKNMFTKSHPQWYPARQSLRLDPKGRSLKDEDILQNLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLVIYLLFYFRVPFIYGP-KYDFITSRHSVVHLACICHSFHYIKRLLETLFVHRFSHGTMPFRNIFKNCMYYWGFAAWMAYYINHPLYTPPTYGEDQVKLAVIIFLFCQLGNFSIHIALRNLRPAGSKTRKIPFPTRNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFTLVGFIQMTIWAKGKHRSYLKEFRDYPPLRSPIVPFIL 346          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Xenopus
Match: tecr (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:493561])

HSP 1 Score: 291.582 bits (745), Expect = 2.537e-96
Identity = 155/306 (50.65%), Postives = 203/306 (66.34%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQK----LAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  + TI DIK +F+  R  +  S   L E +  K    D  I      G+  +LYF+DLG QI W TVFL EY GPL IYLLFYF+  + Y    Y ++ ++     LA  C S HY+KR+LETLF+H+FSH TMP RN+ KNC YYWGFAA++AY+ NH LYTP  YG+ Q+   +++F+  +LGN SIH+   N+RP GS  RKIP+PT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LFT+ GF QMTIWA GKHR+Y KEF +YP  R  I+PF++
Sbjct:   43 EVEILDAKTREKLCFLDKVEPHATIADIKNMFTKSR--FPCSCLTLTEGRSLK----DEDILQNLPVGTTATLYFRDLGAQISWVTVFLTEYAGPLVIYLLFYFRVPFIYGP-KYDFITSRHSVVHLACICHSFHYIKRLLETLFVHRFSHGTMPFRNIFKNCMYYWGFAAWMAYYINHPLYTPPTYGEDQVKLAVIIFLFCQLGNFSIHIALRNLRPAGSKTRKIPFPTRNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFTLVGFIQMTIWAKGKHRSYLKEFRDYPPLRSPIVPFIL 341          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Xenopus
Match: tecr (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:493561])

HSP 1 Score: 280.026 bits (715), Expect = 2.088e-92
Identity = 143/269 (53.16%), Postives = 191/269 (71.00%), Query Frame = 3
Query:  255 KYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQK----LAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            ++ P+RQ L+   + + L D+  + +L   G+  +LYF+DLG QI W TVFL EY GPL IYLLFYF+  + Y    Y ++ ++     LA  C S HY+KR+LETLF+H+FSH TMP RN+ KNC YYWGFAA++AY+ NH LYTP  YG+ Q+   +++F+  +LGN SIH+   N+RP GS  RKIP+PT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LFT+ GF QMTIWA GKHR+Y KEF +YP  R  I+PF++
Sbjct:   29 QWYPARQSLRLDPKGRSLKDEDILQNLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLVIYLLFYFRVPFIYGP-KYDFITSRHSVVHLACICHSFHYIKRLLETLFVHRFSHGTMPFRNIFKNCMYYWGFAAWMAYYINHPLYTPPTYGEDQVKLAVIIFLFCQLGNFSIHIALRNLRPAGSKTRKIPFPTRNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFTLVGFIQMTIWAKGKHRSYLKEFRDYPPLRSPIVPFIL 295          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Xenopus
Match: MGC146850 (uncharacterized protein mgc146850 [Source:NCBI gene;Acc:780081])

HSP 1 Score: 274.633 bits (701), Expect = 2.592e-90
Identity = 149/306 (48.69%), Postives = 205/306 (66.99%), Query Frame = 3
Query:  147 VQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            V+I D +T + L  +  +  + T+ D+K +F           +C K  +  + L D+  + DL   G+  +LYF+DLGPQIGW+ VFL EY GPLF+Y LFYF+  +FY        + H V+   LA  C S HY+KR++ET+F+H+FSH TMPLR+++KNC YYWGFAA++AY+ NH LYTP  YG  Q+   +++F++ E GN SIHV   N+R  G + R+IP+PT NPF++LF FVSCPNYTYEV SW+ ++VM Q +P  LFT+ GF QMTIWA  KH  Y KEF +YP+ R SIIPF++
Sbjct:    8 VEILDCKTMRQLCFFDKVEPHLTVNDVKLMF----------HKCCKYFRLCRSLKDEEILQDLPV-GTTATLYFRDLGPQIGWTMVFLTEYAGPLFVYFLFYFRMPFFYGLAKAFTSSSHSVV--HLACCCHSFHYIKRLIETIFVHRFSHGTMPLRSIVKNCFYYWGFAAWLAYYINHPLYTPPSYGKKQICLAMIMFLICETGNFSIHVALSNLRQDGKS-RRIPFPTRNPFTWLFFFVSCPNYTYEVGSWISFTVMTQCVPVGLFTLIGFIQMTIWAKEKHYRYLKEFKDYPRLRMSIIPFLL 299          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Mouse
Match: Tecr (trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;Acc:MGI:1915408])

HSP 1 Score: 304.294 bits (778), Expect = 6.883e-102
Identity = 158/307 (51.47%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I+D +T + L     +    TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G++++ Q +P  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Mouse
Match: Tecr (trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;Acc:MGI:1915408])

HSP 1 Score: 304.679 bits (779), Expect = 1.140e-101
Identity = 158/307 (51.47%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I+D +T + L     +    TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G++++ Q +P  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:   39 EVEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 342          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Mouse
Match: Tecr (trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;Acc:MGI:1915408])

HSP 1 Score: 286.574 bits (732), Expect = 3.882e-95
Identity = 154/307 (50.16%), Postives = 202/307 (65.80%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I+D +T + L     +    TI +IK LF+                 + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G++++ Q +P  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEIRDAKTREKLCFLDKVEPQATISEIKTLFT---------------KTQGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 293          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Mouse
Match: Tecr (trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;Acc:MGI:1915408])

HSP 1 Score: 281.182 bits (718), Expect = 3.449e-92
Identity = 146/273 (53.48%), Postives = 192/273 (70.33%), Query Frame = 3
Query:  246 DRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            +  ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G++++ Q +P  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:   93 NNPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 362          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Mouse
Match: Tecrl (trans-2,3-enoyl-CoA reductase-like [Source:MGI Symbol;Acc:MGI:2444966])

HSP 1 Score: 224.942 bits (572), Expect = 2.765e-70
Identity = 126/307 (41.04%), Postives = 187/307 (60.91%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGN-----YHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            E++I D  T K +     ++  +TI D+K+ F     K+ PSR  L+      +L D   +  +    S ++LYF DLG Q+GW+TVFLAEY GPL IYLLFY + ++ Y+         H V+   LAF+C   HY++ +LETLF+HK S    P++NL+K C++YWGF +++AY+ NH  YTP  +G+ Q+    + F+  E GN  I+ V  +    GS     P P  NPF++LF  VSCPNYTYE+ SW+ ++VM Q+LP  +FTI    QM++WA  KH+ Y+K+FN+Y   + +IIP ++
Sbjct:   59 EIEILDAHTRKQICIVDKVTQTSTIHDVKQKFHKACPKWYPSRIGLQLEYGGPYLRDYITVQSV-AASSIITLYFTDLGQQVGWTTVFLAEYSGPLLIYLLFYLRSSYIYDVKESTRWPRHPVV--HLAFFCHCIHYIRLLLETLFVHKVSTGHSPMKNLIKGCAFYWGFTSWMAYYINHPRYTPPSFGNRQVIVSAINFLFCEAGNHFINTVLAHPNHTGSNA-CFPSPNYNPFTWLFFLVSCPNYTYEIGSWISFTVMTQTLPVGIFTILMTIQMSLWARKKHKIYRKKFNSYVHRKSAIIPLIL 361          
BLAST of Very-long-chain enoyl-CoA reductase vs. UniProt/SwissProt
Match: sp|Q9N5Y2|TECR_CAEEL (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=6239 GN=art-1 PE=3 SV=1)

HSP 1 Score: 309.686 bits (792), Expect = 3.559e-103
Identity = 159/297 (53.54%), Postives = 206/297 (69.36%), Query Frame = 3
Query:  165 RTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVL--AQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            RT  ++ T   +S + TI  IKK  +  + K    RQ L+   + K L+DD+K+ DL     +  LY +DLGPQI W TVF+AEY GPLF+Y LFY +  + Y     +  +  A ++AF+ WS HY KR+ ET FIH+F +STMP  NL+KNCSYYWGFAAF+AYF NH L+TP  +GDLQ+Y GL  F++ E GNLSIH++  N+RP G+  R+IP P  NP S LF +VSCPNYTYEV SW+ +S+M QSLPA +FT AGF QM IWA GKHR Y KEF +YPK+RK+I+PFV+
Sbjct:   12 RTDNLIITLEGISGSETIKAIKKRIAQKKLKLTEERQALRVEPKGKPLADDQKLSDLGLSSQKAVLYVRDLGPQIAWKTVFMAEYAGPLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIHRFGNSTMPQFNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNLSIHILLRNLRPAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAIIFTTAGFAQMAIWAQGKHRNYLKEFPDYPKNRKAIVPFVL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. UniProt/SwissProt
Match: sp|Q3ZCD7|TECR_BOVIN (Very-long-chain enoyl-CoA reductase OS=Bos taurus OX=9913 GN=TECR PE=2 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 1.408e-101
Identity = 160/307 (52.12%), Postives = 209/307 (68.08%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +    TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEILDAKTREKLCFLDKVEPQATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTRNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. UniProt/SwissProt
Match: sp|Q9NZ01|TECR_HUMAN (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 1.471e-101
Identity = 160/307 (52.12%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  + TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G+++M Q LP  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVV--HLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. UniProt/SwissProt
Match: sp|Q64232|TECR_RAT (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 1.587e-101
Identity = 159/307 (51.79%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I+D +T + L     +    TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G+++M Q +P  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAIFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. UniProt/SwissProt
Match: sp|Q9CY27|TECR_MOUSE (Very-long-chain enoyl-CoA reductase OS=Mus musculus OX=10090 GN=Tecr PE=1 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 4.815e-101
Identity = 158/307 (51.47%), Postives = 210/307 (68.40%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I+D +T + L     +    TI +IK LF+    ++ P+RQ L+   + K L D+  +  L   G+  +LYF+DLG QI W TVFL EY GPLFIYLLFYF+  + Y        + H V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP  YG  Q+   L +F++ +LGN SIH+   ++RP GS  RKIPYPT NPF++LF  VSCPNYTYEV SW+G++++ Q +P  LF++ GF QMTIWA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV-GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVV--HLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRDYPPLRMPIIPFLL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. TrEMBL
Match: A0A1S3IIM5 (very-long-chain enoyl-CoA reductase-like isoform X1 OS=Lingula unguis OX=7574 GN=LOC106164649 PE=4 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 6.656e-105
Identity = 170/306 (55.56%), Postives = 215/306 (70.26%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLR-TEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQ--KLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            ME+++   ++ K LG    LS  +TI D+K  F   +K   P RQ  +  ++SK L D+  +  L  T G +  LYFKDLGPQ+GW+TVFL EY GPL IYLLFY +    Y         A+   +A  CW+ HY KR+LET+F+H+FSH+TMP+ N+ KN SYYWGFAAF+AYF NH LYTP  YG +QLYGGL  FI  ELGN SIH+ F N+RP GS  RKIP PT+NPF++LFT+VSCPNYTYEV +W+ +SVM Q LPA LFT AGF QMT+WA+GKHR YK+EF +YP+ RK IIPF++
Sbjct:    1 MEIEVLHAKSGKSLGKVTGLSPGSTILDVKNEFRKLKKHLYPDRQSFRLEQKSKMLKDEATLKALNITSGGK--LYFKDLGPQVGWTTVFLTEYAGPLVIYLLFYIRPAIIYGASAASKPRAEAVNIAAACWTFHYAKRLLETVFVHRFSHATMPILNICKNSSYYWGFAAFVAYFINHPLYTPPSYGAIQLYGGLAAFIFNELGNFSIHIAFRNLRPAGSKERKIPKPTVNPFTWLFTWVSCPNYTYEVGAWISFSVMTQCLPALLFTAAGFYQMTVWAMGKHRNYKREFKDYPRGRKPIIPFLL 304          
BLAST of Very-long-chain enoyl-CoA reductase vs. TrEMBL
Match: A0A3M7S2D8 (Very-long-chain enoyl-reductase OS=Brachionus plicatilis OX=10195 GN=BpHYR1_028414 PE=4 SV=1)

HSP 1 Score: 318.161 bits (814), Expect = 4.839e-104
Identity = 162/301 (53.82%), Postives = 215/301 (71.43%), Query Frame = 3
Query:  159 DVRTSKVLGTYPNLSANTT--IGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLR--TEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            ++  SK L    N+  N++  I  +KK   + + K  P RQ LK     K L D+ K+ DL   ++  +V LYFKDLGPQ+GW+TVFL EY GPLFIYL+FY + +  Y++ N  Y+    LA  CW  HY KR+LET+F+H+FSH+TMP++N+ KN  YYWGFAA++AY++NH L+TP ++G LQ+Y GL  F+L ELGN SIH+ F ++RP GS VRKIP P  NPF+ LF FVSCPNYTYE+ SW+ +S+M QSLPA LFT+AG  QM +WALGKHR Y+KEF  YP+SR++IIPF +
Sbjct:    6 EILHSKSLKPLFNVQVNSSDSISHVKKAIHSQKSKLYPDRQMLKSDTRGKALKDEEKVADLNKDSDSGKVRLYFKDLGPQVGWTTVFLTEYAGPLFIYLIFYLRPSIIYSNSNAKYLNVTHLACACWVFHYAKRLLETVFVHRFSHATMPIKNIFKNSLYYWGFAAYVAYYNNHPLFTPPLFGSLQIYIGLFSFLLNELGNFSIHLAFRDLRPPGSKVRKIPLPNSNPFTILFNFVSCPNYTYEIGSWISFSIMTQSLPAALFTLAGAYQMIVWALGKHRNYRKEFEKYPRSRRAIIPFFL 306          
BLAST of Very-long-chain enoyl-CoA reductase vs. TrEMBL
Match: A0A224Z226 (Steroid reductase OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 3.110e-103
Identity = 171/306 (55.88%), Postives = 212/306 (69.28%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTE-GSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYND--GNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            MEV+I    + K L     LS +TTI D+KK     + K  P RQC+K   +SK L D+  + DLR + G R  LYFKDLGPQIGW TVFL EY GPL +YLL   +  W Y      ++  +A K+A  CW+ HYVKR+LETLF+H+FSH TMP+ NL KNCSYYW F  +I Y+ NH LYTP   G +Q+YGGL  F+  ELGNLSIH+   N+RP G+  R+IP PT NPF+ LF FVSCPNYTYEV++WL ++ M Q LPA LFT+AGF QMT+WALGKHR YK EF NYP+ RK+I+PF+I
Sbjct:    1 MEVEIVYAGSDKPLAKLHGLSQSTTILDVKKQLHRLKPKLYPDRQCIKSDPKSKALKDESTLGDLRLQSGDR--LYFKDLGPQIGWKTVFLVEYFGPLALYLLTVSRPAWIYGQYASRHNMDVAVKIAAGCWTLHYVKRLLETLFVHRFSHGTMPIMNLFKNCSYYWLFGLYIGYYVNHPLYTPPALGPVQVYGGLAAFLFAELGNLSIHLALRNLRPPGTKERRIPVPTSNPFTLLFEFVSCPNYTYEVMAWLSFTFMTQCLPAGLFTLAGFYQMTVWALGKHRQYKAEFPNYPRQRKAIVPFLI 304          
BLAST of Very-long-chain enoyl-CoA reductase vs. TrEMBL
Match: C3YM48 (S5A_REDUCTASE domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_123019 PE=4 SV=1)

HSP 1 Score: 314.309 bits (804), Expect = 1.111e-102
Identity = 161/303 (53.14%), Postives = 209/303 (68.98%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            ME++I   ++ K +    ++   +T+ D+KK       +Y P+RQ L+   + + LSD+  +  L        LY +DLGPQIGW TVFL EY GP+  YLLFY +  + Y            +AF CW+ HYVKR+LET F+H+FSH TMP+RNL KNCSYYWGFAAF++Y+ NH LYTP  YGD Q+Y  L  F++ ELGNLSIH+ FMNMRP G+  R IPYPT NPF+ LF+ VSCPNYTYEV SW+ +++M Q  PA LFT+AGF QMT+WA+GKHR Y KEF+NYP+SRK IIPF++
Sbjct:    1 MEIEILHAKSGKQITCLDHVDNYSTVLDLKKWIYKQFPRYYPARQSLRLEPKGRNLSDEETVRSLDLRVGDRKLYLRDLGPQIGWKTVFLCEYSGPIAAYLLFYLRPAFIYGVTKAKTHPVVHIAFACWTFHYVKRVLETQFVHRFSHGTMPIRNLFKNCSYYWGFAAFVSYYINHPLYTPPAYGDPQVYSALAGFVINELGNLSIHLAFMNMRPPGTKTRSIPYPTGNPFTSLFSLVSCPNYTYEVGSWVCFTIMTQCFPALLFTVAGFYQMTVWAIGKHRNYLKEFSNYPRSRKPIIPFLL 303          
BLAST of Very-long-chain enoyl-CoA reductase vs. TrEMBL
Match: L7M5J8 (Putative steroid reductase required for elongation of the very long chain fatty acids OS=Rhipicephalus pulchellus OX=72859 PE=2 SV=1)

HSP 1 Score: 313.538 bits (802), Expect = 2.774e-102
Identity = 168/306 (54.90%), Postives = 214/306 (69.93%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTE-GSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYND--GNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            MEV+I  V +SK +     L+ +TTI ++KK     + K  P RQC+K   +SK L D+  + DLR + G R  LYFKDLGPQIGW TVFL EY GPL +YL+   +  W Y      ++  +A K+A  CW+ HYVKR+LETLF+H+FSH TMP+ NL KNCSYYW F  +I Y+ NH LYTP   G +Q+YGGL  F+  ELGNLSIH+   N+RP G+  R+IP PT NPF+ LF FVSCPNYTYEV++WL ++ M Q LPA LFT+AGF QMT+WALGKHR YK EF +YP+ RK+I+PF+I
Sbjct:    1 MEVEIVSVGSSKPVAKLHGLNPSTTILEVKKQLHRLKPKLYPDRQCIKSDPKSKALKDESTLGDLRLQSGDR--LYFKDLGPQIGWKTVFLVEYFGPLALYLVTVSRPAWIYGQYASRHNMDVAVKIAAGCWTLHYVKRLLETLFVHRFSHGTMPIMNLFKNCSYYWLFGLYIGYYVNHPLYTPPALGPVQVYGGLAAFLFAELGNLSIHLALRNLRPPGTKERRIPRPTSNPFTLLFDFVSCPNYTYEVMAWLSFTFMTQCLPAGLFTLAGFYQMTVWALGKHRQYKAEFPDYPRQRKAIVPFLI 304          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Cavefish
Match: tecrb (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:103047753])

HSP 1 Score: 301.212 bits (770), Expect = 7.938e-101
Identity = 155/307 (50.49%), Postives = 209/307 (68.08%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T   L     +  N TIG+IK +F     ++ P+RQ ++   + K L D+  +  L   G+  + YF+DLG QI W TVFL EY GPL IYL+FYF+  + Y        + H+V+   LA  C S HYVKR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP +YG+ Q+   L++F+  ++GN SIH+   N+RP GS  RKIPYPT NPF+++F  VSCPNYTYE+ SWLG+++M Q LP   FT+ GF QMT+WA GKHR+Y KEF +YP  R  I+PF++
Sbjct:    5 EVEILDAKTKDKLCFLDKVEPNATIGEIKSMFHRSHPQWYPARQSIRLDPKGKSLKDEDVLQHLPV-GTTATFYFRDLGAQISWVTVFLTEYAGPLLIYLMFYFRVPFIYAPKYDFTTSKHWVV--HLACMCHSFHYVKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPIYGEQQVKMALIIFLFCQIGNFSIHIALRNLRPPGSKTRKIPYPTKNPFTWIFLLVSCPNYTYELGSWLGFTLMTQCLPVAFFTLVGFIQMTVWAKGKHRSYLKEFRDYPPLRSPILPFIL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Cavefish
Match: tecrb (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:103047753])

HSP 1 Score: 301.982 bits (772), Expect = 1.426e-100
Identity = 155/307 (50.49%), Postives = 209/307 (68.08%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T   L     +  N TIG+IK +F     ++ P+RQ ++   + K L D+  +  L   G+  + YF+DLG QI W TVFL EY GPL IYL+FYF+  + Y        + H+V+   LA  C S HYVKR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP +YG+ Q+   L++F+  ++GN SIH+   N+RP GS  RKIPYPT NPF+++F  VSCPNYTYE+ SWLG+++M Q LP   FT+ GF QMT+WA GKHR+Y KEF +YP  R  I+PF++
Sbjct:   45 EVEILDAKTKDKLCFLDKVEPNATIGEIKSMFHRSHPQWYPARQSIRLDPKGKSLKDEDVLQHLPV-GTTATFYFRDLGAQISWVTVFLTEYAGPLLIYLMFYFRVPFIYAPKYDFTTSKHWVV--HLACMCHSFHYVKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPIYGEQQVKMALIIFLFCQIGNFSIHIALRNLRPPGSKTRKIPYPTKNPFTWIFLLVSCPNYTYELGSWLGFTLMTQCLPVAFFTLVGFIQMTVWAKGKHRSYLKEFRDYPPLRSPILPFIL 348          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Cavefish
Match: tecra (trans-2,3-enoyl-CoA reductase a [Source:ZFIN;Acc:ZDB-GENE-090930-1])

HSP 1 Score: 288.115 bits (736), Expect = 1.076e-95
Identity = 152/307 (49.51%), Postives = 205/307 (66.78%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +    TI DIK +F    +++ P+RQ L    + K L D+  +  +   G+  + YF+DLG QI W TVFL+E  GPL IYL+FYF+  + Y        + H+V+   LA  C S HYVKRILETLF+H+FSH TMPLRN+ KNCSYYW FAA++AY+ NH LYTP  YG+ Q+   L +F+  +LG+ SIHV   N++P GS ++KIPYPT NPF+++F  VSCPNYTYEV +W+G++VM Q +P   FT+ GF QMT+WA GKHR+Y KEF  YP  R  I+PF++
Sbjct:    5 EVEILDAKTKEKLLLLDKVEPIATILDIKSMFHKSNQRWYPARQSLCLDPKGKPLKDE-DVLQMLPVGTTATFYFRDLGAQISWGTVFLSECIGPLVIYLMFYFRVPFIYAPKYDFKTSKHWVV--HLACLCHSFHYVKRILETLFVHRFSHGTMPLRNIFKNCSYYWFFAAWMAYYINHPLYTPPYYGEQQVKTALGIFLFCQLGSFSIHVTLRNLKPPGSKIKKIPYPTKNPFTWIFALVSCPNYTYEVGTWMGFTVMTQCVPVAFFTVIGFIQMTVWAKGKHRSYLKEFKEYPTLRSPILPFIL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Cavefish
Match: tecra (trans-2,3-enoyl-CoA reductase a [Source:ZFIN;Acc:ZDB-GENE-090930-1])

HSP 1 Score: 289.271 bits (739), Expect = 4.369e-95
Identity = 152/307 (49.51%), Postives = 205/307 (66.78%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +    TI DIK +F    +++ P+RQ L    + K L D+  +  +   G+  + YF+DLG QI W TVFL+E  GPL IYL+FYF+  + Y        + H+V+   LA  C S HYVKRILETLF+H+FSH TMPLRN+ KNCSYYW FAA++AY+ NH LYTP  YG+ Q+   L +F+  +LG+ SIHV   N++P GS ++KIPYPT NPF+++F  VSCPNYTYEV +W+G++VM Q +P   FT+ GF QMT+WA GKHR+Y KEF  YP  R  I+PF++
Sbjct:   39 EVEILDAKTKEKLLLLDKVEPIATILDIKSMFHKSNQRWYPARQSLCLDPKGKPLKDE-DVLQMLPVGTTATFYFRDLGAQISWGTVFLSECIGPLVIYLMFYFRVPFIYAPKYDFKTSKHWVV--HLACLCHSFHYVKRILETLFVHRFSHGTMPLRNIFKNCSYYWFFAAWMAYYINHPLYTPPYYGEQQVKTALGIFLFCQLGSFSIHVTLRNLKPPGSKIKKIPYPTKNPFTWIFALVSCPNYTYEVGTWMGFTVMTQCVPVAFFTVIGFIQMTVWAKGKHRSYLKEFKEYPTLRSPILPFIL 342          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Cavefish
Match: tecrb (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:103047753])

HSP 1 Score: 277.715 bits (709), Expect = 1.252e-91
Identity = 150/307 (48.86%), Postives = 202/307 (65.80%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T   L     +      G       T   ++ P+RQ ++   + K L D+  +  L   G+  + YF+DLG QI W TVFL EY GPL IYL+FYF+  + Y        + H+V+   LA  C S HYVKR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP +YG+ Q+   L++F+  ++GN SIH+   N+RP GS  RKIPYPT NPF+++F  VSCPNYTYE+ SWLG+++M Q LP   FT+ GF QMT+WA GKHR+Y KEF +YP  R  I+PF++
Sbjct:   14 EVEILDAKTKDKLCFLDKVRPALHSG-------TFYPQWYPARQSIRLDPKGKSLKDEDVLQHLPV-GTTATFYFRDLGAQISWVTVFLTEYAGPLLIYLMFYFRVPFIYAPKYDFTTSKHWVV--HLACMCHSFHYVKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPIYGEQQVKMALIIFLFCQIGNFSIHIALRNLRPPGSKTRKIPYPTKNPFTWIFLLVSCPNYTYELGSWLGFTLMTQCLPVAFFTLVGFIQMTVWAKGKHRSYLKEFRDYPPLRSPILPFIL 310          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Sea Lamprey
Match: srd5a2b (steroid-5-alpha-reductase, alpha polypeptide 2b [Source:ZFIN;Acc:ZDB-GENE-050417-182])

HSP 1 Score: 66.2402 bits (160), Expect = 9.588e-13
Identity = 35/93 (37.63%), Postives = 53/93 (56.99%), Query Frame = 3
Query:  765 VFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPF 1043
            +  N+R  G  V +IP         LF  VS  N+  E++ W+GY++   + PAF F I   G +   AL  HR Y ++F++YPKSRK++IP+
Sbjct:  170 ILRNLRKPGEYVYRIPR------GGLFELVSGANFLGEIVEWIGYALATWTFPAFAFAIFTIGCIGPRALHHHRYYHEKFSDYPKSRKALIPY 256          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Sea Lamprey
Match: srd5a1 (steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) [Source:ZFIN;Acc:ZDB-GENE-060929-938])

HSP 1 Score: 60.4622 bits (145), Expect = 3.529e-11
Identity = 31/94 (32.98%), Postives = 54/94 (57.45%), Query Frame = 3
Query:  765 VFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFV 1046
            +  N+R  G T  KIP         +F FVS  N+  E++ WLG+++ C +L A+ F++     +   ++  H+ Y ++F +YPK RK++IPF+
Sbjct:   99 ILRNLRKPGETGYKIPR------GGMFEFVSGANFFGEMVEWLGFAIACCTLQAWAFSVFSITVIGPRSVHHHKWYLEKFEDYPKKRKAVIPFL 186          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Yeast
Match: TSC13 (Enoyl reductase; catalyzes last step in each cycle of very long chain fatty acid elongation; localizes to ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Elo2p and Elo3p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress; human homolog TECR implicated in nonsyndromic mental retardation, can complement yeast mutant [Source:SGD;Acc:S000002173])

HSP 1 Score: 111.309 bits (277), Expect = 1.329e-28
Identity = 94/323 (29.10%), Postives = 146/323 (45.20%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRN-------WFYNDGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAY--------FHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNM-----RPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQ-SLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFV 1046
            M + IK  R+  +  T  +LS   T+ D+ K  S +       R  L   KESK +    +          +  + KDLGPQI W  VF  EY GP+ ++ LFY+          W     +Y+  L  ++A++    HY KR+ ETLF+H+FS +TMP+ NL KNC +YW  +  I++        F N +L+    Y  L     L+   +          + + +     +  G+   ++P   LN    +F     PNYT+EV SW+ ++ + + +L A LF      QM  WA  K++ Y          R  +IPFV
Sbjct:    1 MPITIKS-RSKGLRDTEIDLSKKPTLDDVLKKISANNHNISKYRIRLTYKKESKQVPVISESFFQEEADDSMEFFIKDLGPQISWRLVFFCEYLGPVLVHSLFYYLSTIPTVVDRWHSASSDYNPFL-NRVAYFLILGHYGKRLFETLFVHQFSLATMPIFNLFKNCFHYWVLSGLISFGYFGYGFPFGNAKLFKYYSYLKLDDLSTLIGLFVLSELWNFYCHIKLRLWGDYQKKHGNAKIRVP---LN--QGIFNLFVAPNYTFEVWSWIWFTFVFKFNLFAVLFLTVSTAQMYAWAQKKNKKYHTR-------RAFLIPFV 309          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Nematostella
Match: EDO28659 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5V1])

HSP 1 Score: 226.098 bits (575), Expect = 7.611e-73
Identity = 119/232 (51.29%), Postives = 152/232 (65.52%), Query Frame = 3
Query:  372 DLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQ--KLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVL--SW--LGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            D G QI W+TVF+AEY GPL +YL  Y + ++ Y        +AQ   +A  CWS HYVKR+LET+F+H+FS  TMP+ NL KNCSYYWGF   + YF NH LYTP    +L L   ++      L    IH +  ++RP GS  R+IPY   NP + LF FVSCPNYTYE +   W  + +  +C S+PA LFT+AGF QMT+WALGKHR YKKEF +YP+ R++IIPF I
Sbjct:    6 DTGVQISWTTVFMAEYAGPLAVYLFLYARPSFIYGADAASKPVAQVVNIAAACWSFHYVKRLLETMFVHRFSKGTMPIFNLFKNCSYYWGFGFLVGYFINHPLYTPP---NLGLKMKVIAITDCSLFFFVIHTLLRDLRPPGSHERRIPYANSNPMTQLFRFVSCPNYTYEAIFFCWCRVTFPTLCVSIPAALFTLAGFYQMTVWALGKHRNYKKEFPDYPRRRRAIIPFFI 234          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Nematostella
Match: EDO40603 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6S0])

HSP 1 Score: 205.297 bits (521), Expect = 2.032e-65
Identity = 108/182 (59.34%), Postives = 136/182 (74.73%), Query Frame = 3
Query:  504 QKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            Q +A  CWS HYVKR+LET+F+H+FS  TMP+ NL KNCSYYWGF   + YF NH LYTP  +GD+Q+YG L  F++ ELGN SIH +  ++RP GS  R+IPY   NP + LF FVSCPNYTYE  +W+ ++VM Q+    LFT+AGF QMT+WALGKHR YKKEF +YP+ R++IIPF I
Sbjct:   12 QNIAAACWSFHYVKRLLETMFVHRFSKGTMPIFNLFKNCSYYWGFGFLVGYFINHPLYTPPKFGDMQVYGSLAGFLICELGNFSIHTLLRDLRPPGSHERRIPYANSNPMTQLFRFVSCPNYTYETGAWICFTVMTQTFGTALFTLAGFYQMTVWALGKHRNYKKEFPDYPRRRRAIIPFFI 193          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Nematostella
Match: EDO37345 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFY9])

HSP 1 Score: 62.3882 bits (150), Expect = 1.217e-11
Identity = 46/185 (24.86%), Postives = 82/185 (44.32%), Query Frame = 3
Query:  507 KLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSI-----HVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFV 1046
            K+A   + AH+VKRILE +F+HK+S         L +   ++   A I ++  +   +      L     L+   L       +     H++  N+RP+GS   K   PT      LF    CP+Y  E+++WLG ++  Q    +L  +     +   +      Y K+  N+P+ R +++P +
Sbjct:    1 KIALASYVAHFVKRILECIFLHKYSRDIE-----LSSIGAFYSLGAVIQHYWCNMYTSDTTAQRLSNNQLLITAGLIVYAIGEMTNFQHHLILANLRPVGSAAGKYAVPT----GGLFDVAICPHYFGELMAWLGMAIAGQHFALYLSWLTMVAYLAGRSHQTRHWYIKKIENFPQDRWNLLPGI 176          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Nematostella
Match: EDO43991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RX92])

HSP 1 Score: 56.6102 bits (135), Expect = 4.369e-10
Identity = 25/68 (36.76%), Postives = 40/68 (58.82%), Query Frame = 3
Query:  843 FTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFV 1046
            F  VSCPNY  E+L W G+++   S    ++ + G G     A+  H+ YK  F +YP+ R+++IPF+
Sbjct:   59 FELVSCPNYFGEMLEWFGWALCTWSAAGLVWFLFGCGTFIPRAMHNHKWYKDRFPDYPQKRRALIPFL 126          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Nematostella
Match: EDO29081 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4N2])

HSP 1 Score: 52.7582 bits (125), Expect = 3.569e-9
Identity = 29/95 (30.53%), Postives = 49/95 (51.58%), Query Frame = 3
Query:  765 VFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            +  + R  G    KIP         LF +VS  N+  E+L W G+++   S P F +       +   A+  H+ Y ++F +YPKSRK++IP+++
Sbjct:    2 ILTSXRKPGEVGYKIPR------GGLFEYVSGANFCSEILEWSGFALASFSYPGFTYAFFTLCNVGPRAIQHHKFYVEKFEDYPKSRKALIPWLL 90          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Medaka
Match: tecrb (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:101155407])

HSP 1 Score: 297.36 bits (760), Expect = 2.982e-99
Identity = 156/307 (50.81%), Postives = 209/307 (68.08%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +  N TIG+IK +F     ++ P+RQ ++   + K L D+  +  L   G+  + YF+DLG QI W TVFL EY GPL IYLLFYF+  + Y        + H+V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP +YG+ Q+   L+LF+  ++GN SIH+   N+RP GS  RKIPYPT NPF+++   VSCPNYTYE+ SWLG+++M Q LP   FT+ GF QMT+WA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEILDAKTKEKLCFLDKVEPNATIGEIKSMFHRTHPQWYPARQSIRLDPKGKSLKDEDVLQHLPV-GTTATFYFRDLGAQISWVTVFLTEYTGPLVIYLLFYFRVPFIYAAKYDFTTSKHWVV--HLACMCHSFHYMKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPIYGEQQIRLALILFLFCQIGNFSIHIALRNLRPPGSKTRKIPYPTKNPFTWISLLVSCPNYTYELGSWLGFTLMTQCLPVAFFTLVGFIQMTVWAKGKHRSYLKEFRDYPPLRSPIIPFIL 308          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Medaka
Match: tecrl2a (very-long-chain enoyl-CoA reductase-like [Source:NCBI gene;Acc:101171706])

HSP 1 Score: 279.641 bits (714), Expect = 4.303e-92
Identity = 145/307 (47.23%), Postives = 212/307 (69.06%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYND-----GNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV++ D +T + L     +  ++TIGDIK LF     ++ P+RQ L    + KFL D+  +  L   G+  ++YFKDLGPQ+GW+TVF AE  GPL IYLLFYF+  + Y+D      + H V+   +A  C + HY+KR++ET+F+H+FSH +MPLR ++KNC YYWGF+A++AY+ NH LYTP  YG++Q++  +++F + E+GN SIH+   ++R   S  R+ P P+ NPF++LF FVSCPN+TYEV +W+ +S+M Q LP  +FT+ GF QMT+WA GKHRAY +EF +YP  R +IIP ++
Sbjct:   25 EVEVLDAKTREQLCFLDKVEPHSTIGDIKSLFYKLYPRWYPARQSLTLDPKGKFLRDEEILQSLPV-GTTATMYFKDLGPQLGWTTVFFAENIGPLLIYLLFYFRVPYIYSDRCAFTSSPHKVVT--MACVCHTFHYLKRLIETIFVHRFSHGSMPLRTIVKNCLYYWGFSAWLAYYINHPLYTPPSYGEVQVHYAMVIFAICEMGNFSIHLSLSHLRGDRSRGRRFPAPSKNPFTWLFFFVSCPNHTYEVGTWVSFSIMTQCLPVAVFTVLGFIQMTVWAKGKHRAYVREFKDYPAVRVAIIPLIL 328          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Medaka
Match: tecrb (trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Acc:101155407])

HSP 1 Score: 274.633 bits (701), Expect = 2.142e-90
Identity = 153/307 (49.84%), Postives = 204/307 (66.45%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV+I D +T + L     +     +  I  L   D + Y P+RQ ++   + K L D+  +  L   G+  + YF+DLG QI W TVFL EY GPL IYLLFYF+  + Y        + H+V+   LA  C S HY+KR+LETLF+H+FSH TMPLRN+ KNC+YYWGFAA++AY+ NH LYTP +YG+ Q+   L+LF+  ++GN SIH+   N+RP GS  RKIPYPT NPF+++   VSCPNYTYE+ SWLG+++M Q LP   FT+ GF QMT+WA GKHR+Y KEF +YP  R  IIPF++
Sbjct:    5 EVEILDAKTKEKLCFLDKV--RNKLQRILYLPVPDPQWY-PARQSIRLDPKGKSLKDEDVLQHLPV-GTTATFYFRDLGAQISWVTVFLTEYTGPLVIYLLFYFRVPFIYAAKYDFTTSKHWVV--HLACMCHSFHYMKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPIYGEQQIRLALILFLFCQIGNFSIHIALRNLRPPGSKTRKIPYPTKNPFTWISLLVSCPNYTYELGSWLGFTLMTQCLPVAFFTLVGFIQMTVWAKGKHRSYLKEFRDYPPLRSPIIPFIL 305          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Medaka
Match: tecrl2a (very-long-chain enoyl-CoA reductase-like [Source:NCBI gene;Acc:101171706])

HSP 1 Score: 258.84 bits (660), Expect = 3.641e-84
Identity = 140/314 (44.59%), Postives = 206/314 (65.61%), Query Frame = 3
Query:  144 EVQIKDVRTSKVLGTYPNLSANT-------TIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYND-----GNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            EV++ D +T + L     + +N         I D          ++ P+RQ L    + KFL D+  +  L   G+  ++YFKDLGPQ+GW+TVF AE  GPL IYLLFYF+  + Y+D      + H V+   +A  C + HY+KR++ET+F+H+FSH +MPLR ++KNC YYWGF+A++AY+ NH LYTP  YG++Q++  +++F + E+GN SIH+   ++R   S  R+ P P+ NPF++LF FVSCPN+TYEV +W+ +S+M Q LP  +FT+ GF QMT+WA GKHRAY +EF +YP  R +IIP ++
Sbjct:    8 EVEVLDAKTREQLCFLDKVRSNIHSVISYLKIIDFIANIRFTYPRWYPARQSLTLDPKGKFLRDEEILQSLPV-GTTATMYFKDLGPQLGWTTVFFAENIGPLLIYLLFYFRVPYIYSDRCAFTSSPHKVVT--MACVCHTFHYLKRLIETIFVHRFSHGSMPLRTIVKNCLYYWGFSAWLAYYINHPLYTPPSYGEVQVHYAMVIFAICEMGNFSIHLSLSHLRGDRSRGRRFPAPSKNPFTWLFFFVSCPNHTYEVGTWVSFSIMTQCLPVAVFTVLGFIQMTVWAKGKHRAYVREFKDYPAVRVAIIPLIL 318          
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Medaka
Match: tecra (very-long-chain enoyl-CoA reductase [Source:NCBI gene;Acc:101163495])

HSP 1 Score: 255.373 bits (651), Expect = 1.432e-82
Identity = 133/250 (53.20%), Postives = 176/250 (70.40%), Query Frame = 3
Query:  315 DRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYN-----DGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            D ++      G+  S YF DLGPQ+ W TVFLAE  GPL +YL+FYF+  + Y+       + H+V+   LA  C S HY+KRILETLF+H+ SH TMPLRN+ KNC YYW  AA++AY+ NH LYT  +YG  Q+  GL +F+  ++GN SIHVV  N++  GS V+KIPYPT NPF+++F  VSCPNYTYE+ SW+G++VM Q +P  LFT+  F QMT+WA GKHR+Y KEF +YP  R SI+PF++
Sbjct:   78 DEEVLQTLPVGTTASFYFSDLGPQLTWGTVFLAESVGPLVLYLMFYFRLPFIYSPKYDFTSSKHWVV--HLACMCHSFHYIKRILETLFVHRISHGTMPLRNIFKNCGYYWCSAAWMAYYINHPLYTTPLYGQQQVNAGLYIFLFCQVGNFSIHVVLRNLKQPGSKVKKIPYPTRNPFTWIFWLVSCPNYTYELGSWIGFTVMTQCVPVALFTLVAFIQMTVWAKGKHRSYLKEFRDYPTLRSSILPFIV 325          
BLAST of Very-long-chain enoyl-CoA reductase vs. Planmine SMEST
Match: SMESG000020075.1 (SMESG000020075.1)

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 301/303 (99.34%), Postives = 302/303 (99.67%), Query Frame = 3
Query:  141 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESK+SKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQKLAFYCWSAHY KRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYGDLQLYGGLLLFILGELGNLSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI
Sbjct:    1 MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKESKDSKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIYLLFYFKRNWFYNDGNYHYVLAQKLAFYCWSAHYAKRILETLFIHKFSHSTMPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYGDLQLYGGLLLFILGELGNLSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 303          
BLAST of Very-long-chain enoyl-CoA reductase vs. Planmine SMEST
Match: SMESG000020075.1 (SMESG000020075.1)

HSP 1 Score: 271.552 bits (693), Expect = 1.213e-91
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 3
Query:  591 MPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYXXXXXXXXXXXXXXXXXXXXSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            MPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYGDLQLYGGLLLFILGELGNLSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI
Sbjct:    1 MPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYGDLQLYGGLLLFILGELGNLSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 153          
BLAST of Very-long-chain enoyl-CoA reductase vs. Planmine SMEST
Match: SMESG000078577.1 (SMESG000078577.1)

HSP 1 Score: 61.2326 bits (147), Expect = 9.215e-11
Identity = 28/74 (37.84%), Postives = 45/74 (60.81%), Query Frame = 3
Query:  828 PFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            P  +LF +VSC NY  E++ WL Y++  QSL +  F +     +   ++  HR Y  +F  YP++RK+IIPF++
Sbjct:  132 PKGFLFEYVSCANYFGEIVEWLSYALAMQSLASIAFAMFTVFNLLPRSISHHRWYVVKFEKYPRNRKAIIPFIL 205          
BLAST of Very-long-chain enoyl-CoA reductase vs. Planmine SMEST
Match: SMESG000078577.1 (SMESG000078577.1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.606e-10
Identity = 28/74 (37.84%), Postives = 45/74 (60.81%), Query Frame = 3
Query:  828 PFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            P  +LF +VSC NY  E++ WL Y++  QSL +  F +     +   ++  HR Y  +F  YP++RK+IIPF++
Sbjct:  162 PKGFLFEYVSCANYFGEIVEWLSYALAMQSLASIAFAMFTVFNLLPRSISHHRWYVVKFEKYPRNRKAIIPFIL 235          
BLAST of Very-long-chain enoyl-CoA reductase vs. Planmine SMEST
Match: SMESG000078577.1 (SMESG000078577.1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.815e-10
Identity = 28/74 (37.84%), Postives = 45/74 (60.81%), Query Frame = 3
Query:  828 PFSYLFTFVSCPNYTYEVLSWLGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIPFVI 1049
            P  +LF +VSC NY  E++ WL Y++  QSL +  F +     +   ++  HR Y  +F  YP++RK+IIPF++
Sbjct:  166 PKGFLFEYVSCANYFGEIVEWLSYALAMQSLASIAFAMFTVFNLLPRSISHHRWYVVKFEKYPRNRKAIIPFIL 239          
The following BLAST results are available for this feature:
BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TECR3.063e-10252.12trans-2,3-enoyl-CoA reductase [Source:HGNC Symbol;... [more]
TECR8.453e-10252.12trans-2,3-enoyl-CoA reductase [Source:HGNC Symbol;... [more]
TECRL9.913e-6740.33trans-2,3-enoyl-CoA reductase like [Source:HGNC Sy... [more]
TECRL2.332e-5440.98trans-2,3-enoyl-CoA reductase like [Source:HGNC Sy... [more]
TECR2.512e-3961.62trans-2,3-enoyl-CoA reductase [Source:HGNC Symbol;... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
art-12.798e-10453.54Probable very-long-chain enoyl-CoA reductase art-1... [more]
F42F12.31.107e-1435.11pep chromosome:WBcel235:X:12350578:12351788:1 gene... [more]
F19H6.44.760e-1334.41pep chromosome:WBcel235:X:12365688:12367255:1 gene... [more]
ZK1251.34.116e-835.14pep chromosome:WBcel235:IV:9683617:9684473:1 gene:... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sc29.640e-8948.20gene:FBgn0035471 transcript:FBtr0073159[more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tecrb1.079e-10050.49trans-2,3-enoyl-CoA reductase b [Source:ZFIN;Acc:Z... [more]
tecra5.430e-8847.54trans-2,3-enoyl-CoA reductase a [Source:ZFIN;Acc:Z... [more]
tecrl2b2.340e-8649.34trans-2,3-enoyl-CoA reductase-like 2b [Source:ZFIN... [more]
tecrl2a2.521e-6056.25trans-2,3-enoyl-CoA reductase-like 2a [Source:ZFIN... [more]
tecrl2a1.861e-5954.12trans-2,3-enoyl-CoA reductase-like 2a [Source:ZFIN... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tecr3.019e-10150.65trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecr3.396e-10150.65trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecr2.537e-9650.65trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecr2.088e-9253.16trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
MGC1468502.592e-9048.69uncharacterized protein mgc146850 [Source:NCBI gen... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tecr6.883e-10251.47trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;A... [more]
Tecr1.140e-10151.47trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;A... [more]
Tecr3.882e-9550.16trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;A... [more]
Tecr3.449e-9253.48trans-2,3-enoyl-CoA reductase [Source:MGI Symbol;A... [more]
Tecrl2.765e-7041.04trans-2,3-enoyl-CoA reductase-like [Source:MGI Sym... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9N5Y2|TECR_CAEEL3.559e-10353.54Probable very-long-chain enoyl-CoA reductase art-1... [more]
sp|Q3ZCD7|TECR_BOVIN1.408e-10152.12Very-long-chain enoyl-CoA reductase OS=Bos taurus ... [more]
sp|Q9NZ01|TECR_HUMAN1.471e-10152.12Very-long-chain enoyl-CoA reductase OS=Homo sapien... [more]
sp|Q64232|TECR_RAT1.587e-10151.79Very-long-chain enoyl-CoA reductase OS=Rattus norv... [more]
sp|Q9CY27|TECR_MOUSE4.815e-10151.47Very-long-chain enoyl-CoA reductase OS=Mus musculu... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3IIM56.656e-10555.56very-long-chain enoyl-CoA reductase-like isoform X... [more]
A0A3M7S2D84.839e-10453.82Very-long-chain enoyl-reductase OS=Brachionus plic... [more]
A0A224Z2263.110e-10355.88Steroid reductase OS=Rhipicephalus zambeziensis OX... [more]
C3YM481.111e-10253.14S5A_REDUCTASE domain-containing protein OS=Branchi... [more]
L7M5J82.774e-10254.90Putative steroid reductase required for elongation... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tecrb7.938e-10150.49trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecrb1.426e-10050.49trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecra1.076e-9549.51trans-2,3-enoyl-CoA reductase a [Source:ZFIN;Acc:Z... [more]
tecra4.369e-9549.51trans-2,3-enoyl-CoA reductase a [Source:ZFIN;Acc:Z... [more]
tecrb1.252e-9148.86trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
srd5a2b9.588e-1337.63steroid-5-alpha-reductase, alpha polypeptide 2b [S... [more]
srd5a13.529e-1132.98steroid-5-alpha-reductase, alpha polypeptide 1 (3-... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
TSC131.329e-2829.10Enoyl reductase; catalyzes last step in each cycle... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO286597.611e-7351.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO406032.032e-6559.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO373451.217e-1124.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO439914.369e-1036.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO290813.569e-930.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tecrb2.982e-9950.81trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecrl2a4.303e-9247.23very-long-chain enoyl-CoA reductase-like [Source:N... [more]
tecrb2.142e-9049.84trans-2,3-enoyl-CoA reductase [Source:NCBI gene;Ac... [more]
tecrl2a3.641e-8444.59very-long-chain enoyl-CoA reductase-like [Source:N... [more]
tecra1.432e-8253.20very-long-chain enoyl-CoA reductase [Source:NCBI g... [more]
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BLAST of Very-long-chain enoyl-CoA reductase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020075.10.000e+099.34SMESG000020075.1[more]
SMESG000020075.11.213e-91100.00SMESG000020075.1[more]
SMESG000078577.19.215e-1137.84SMESG000078577.1[more]
SMESG000078577.11.606e-1037.84SMESG000078577.1[more]
SMESG000078577.11.815e-1037.84SMESG000078577.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007750 ID=SMED30007750|Name=Very-long-chain enoyl-CoA reductase|organism=Schmidtea mediterranea sexual|type=transcript|length=1101bp
TCTTAATAAAATTGGTTGTTTAAGTTTTAATCTTTGAGAAACATTTTAAT
TTAGTAAACGAACTATCAACCTCGATTATATTCAAATATTAAATTAAACG
GCTTCAATAATTTTAAATTACAATTTTTGTCAACAGTTTAATGGAAGTAC
AAATAAAAGATGTTAGAACTTCAAAGGTTTTAGGAACATATCCAAATCTT
AGCGCGAATACGACAATAGGAGATATAAAAAAGCTTTTTTCGACAGATAG
GAAAAAGTATCAACCTAGTCGACAATGTTTAAAAGAATCTAAAGAAAGCA
AATTTCTGTCGGATGACAGGAAAATTTGCGACCTTCGAACTGAAGGTAGT
CGAGTGAGTCTTTATTTTAAAGATCTCGGACCCCAAATAGGATGGAGCAC
AGTTTTCCTCGCTGAATACGGAGGACCGTTGTTTATCTATTTGTTATTTT
ATTTCAAGCGAAATTGGTTTTATAATGATGGGAATTATCACTATGTTCTG
GCACAAAAATTGGCCTTCTATTGTTGGAGTGCCCATTATGTTAAAAGGAT
ATTGGAGACTTTGTTCATTCATAAATTTTCCCATTCAACAATGCCGCTTA
GGAATTTACTGAAGAATTGTTCCTATTATTGGGGATTCGCAGCATTTATT
GCATATTTCCACAACCATCAGTTGTATACACCAGCAGTGTACGGAGATCT
ACAATTATACGGTGGATTGTTGTTATTTATCTTAGGTGAATTGGGCAACT
TGTCTATTCATGTGGTTTTCATGAATATGAGGCCAATGGGATCAACTGTC
CGTAAAATACCCTATCCAACATTAAATCCATTCAGCTATCTGTTTACATT
CGTATCGTGCCCGAATTACACATACGAAGTGCTGTCCTGGTTAGGATATT
CCGTTATGTGCCAATCGCTACCGGCGTTCTTATTCACAATTGCCGGATTC
GGACAAATGACTATTTGGGCTCTTGGTAAACATAGAGCTTATAAAAAGGA
GTTCAATAATTATCCGAAGTCGAGAAAATCAATCATTCCGTTTGTTATTT
AATCAATTACTATTAGGTTTTAGTTAATAAATTTTGAAATTTAAAAAAAA
A
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protein sequence of SMED30007750-orf-1

>SMED30007750-orf-1 ID=SMED30007750-orf-1|Name=SMED30007750-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=304bp
MEVQIKDVRTSKVLGTYPNLSANTTIGDIKKLFSTDRKKYQPSRQCLKES
KESKFLSDDRKICDLRTEGSRVSLYFKDLGPQIGWSTVFLAEYGGPLFIY
LLFYFKRNWFYNDGNYHYVLAQKLAFYCWSAHYVKRILETLFIHKFSHST
MPLRNLLKNCSYYWGFAAFIAYFHNHQLYTPAVYGDLQLYGGLLLFILGE
LGNLSIHVVFMNMRPMGSTVRKIPYPTLNPFSYLFTFVSCPNYTYEVLSW
LGYSVMCQSLPAFLFTIAGFGQMTIWALGKHRAYKKEFNNYPKSRKSIIP
FVI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR039357SRD5A/TECR
IPR0011043-oxo-5_a-steroid_4-DH_C
Vocabulary: biological process
TermDefinition
GO:0006629lipid metabolic process
GO:0006631fatty acid metabolic process
GO:0006633fatty acid biosynthetic process
GO:0030497fatty acid elongation
GO:0035338long-chain fatty-acyl-CoA biosynthetic process
GO:0042761very long-chain fatty acid biosynthetic process
GO:0055114oxidation-reduction process
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0005737cytoplasm
GO:0005634nucleus
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0016020membrane
GO:0030176integral component of endoplasmic reticulum membrane
Vocabulary: molecular function
TermDefinition
GO:0016627oxidoreductase activity, acting on the CH-CH group of donors
GO:0005515protein binding
GO:0016491oxidoreductase activity
GO:0017099very-long-chain-acyl-CoA dehydrogenase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 1..79
e-value: 2.8E-15
score: 57.7
NoneNo IPR availablePANTHERPTHR10556:SF28SC2coord: 1..303
NoneNo IPR availableCDDcd01801Ubl_TECR_likecoord: 2..79
e-value: 3.95462E-13
score: 61.5335
NoneNo IPR availableTMHMMTMhelixcoord: 83..105
NoneNo IPR availableTMHMMTMhelixcoord: 155..174
NoneNo IPR availableTMHMMTMhelixcoord: 260..279
NoneNo IPR availableTMHMMTMhelixcoord: 223..245
NoneNo IPR availableTMHMMTMhelixcoord: 189..211
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 150..302
e-value: 3.7E-32
score: 111.4
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 192..278
score: 24.812
IPR0393573-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductasePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 1..303