U2 snRNP auxiliary factor large subunit
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007734 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11Note: Hover over icons to view figure legend
Homology
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156]) HSP 1 Score: 390.578 bits (1002), Expect = 2.576e-131 Identity = 204/387 (52.71%), Postives = 274/387 (70.80%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 471
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156]) HSP 1 Score: 389.808 bits (1000), Expect = 6.396e-131 Identity = 204/391 (52.17%), Postives = 274/391 (70.08%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA------SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156]) HSP 1 Score: 318.546 bits (815), Expect = 1.587e-105 Identity = 173/308 (56.17%), Postives = 223/308 (72.40%), Query Frame = 2
Query: 539 MMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 1 MMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 307
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156]) HSP 1 Score: 73.559 bits (179), Expect = 1.278e-15 Identity = 30/77 (38.96%), Postives = 50/77 (64.94%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTE 532
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TE
Sbjct: 10 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITE 86
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042]) HSP 1 Score: 75.485 bits (184), Expect = 6.795e-14 Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2
Query: 1124 KISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQI-PRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRN 1438
K+ +E+ V+ L NMV D+ DD E E V EEC KFG V V I G +++ + KIFVEF SET KA++AL+GR F+G+ VV YD E++ N
Sbjct: 454 KLLRKQESTVMVLRNMVDPKDIDDDLEGE-----VTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDN 554 HSP 2 Score: 59.3066 bits (142), Expect = 9.703e-9 Identity = 45/192 (23.44%), Postives = 84/192 (43.75%), Query Frame = 2
Query: 494 RIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTD------KNFAFLEFRSVEETNQGL-NLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRAC 1048
R+YVG++ + E+ + + F A PI ++ ++ D K FAF+E+ E L ++ ++ G +K+ RP + D + + ++++V + Q L +D +K + + FG ++ L +D TTG KGY F EY + A + LG + + V +A
Sbjct: 130 RVYVGSIYYELGEDTIRQAF-----------APFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAF-------NRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAV 303
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978]) HSP 1 Score: 358.221 bits (918), Expect = 3.880e-119 Identity = 186/382 (48.69%), Postives = 255/382 (66.75%), Query Frame = 2
Query: 305 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLG-AKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
K Y++WDV PTG+E + E+K + + GQV G S P +G +V+ Q+RR+YVGN+P G EE M++FFN+Q+ L L QA G+PI+ QIN DKNFAF+EFRS++ET G+ DGI + G LK+RRPRDY P FD + S + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+L+ D+EYEEI+EDV++ECSK+G V S++IPRP D V GVGK+FVEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 109 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV------------PRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 471
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978]) HSP 1 Score: 358.221 bits (918), Expect = 4.038e-119 Identity = 186/382 (48.69%), Postives = 255/382 (66.75%), Query Frame = 2
Query: 305 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLG-AKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
K Y++WDV PTG+E + E+K + + GQV G S P +G +V+ Q+RR+YVGN+P G EE M++FFN+Q+ L L QA G+PI+ QIN DKNFAF+EFRS++ET G+ DGI + G LK+RRPRDY P FD + S + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+L+ D+EYEEI+EDV++ECSK+G V S++IPRP D V GVGK+FVEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 112 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV------------PRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 474
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978]) HSP 1 Score: 358.221 bits (918), Expect = 7.984e-119 Identity = 186/382 (48.69%), Postives = 255/382 (66.75%), Query Frame = 2
Query: 305 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLG-AKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
K Y++WDV PTG+E + E+K + + GQV G S P +G +V+ Q+RR+YVGN+P G EE M++FFN+Q+ L L QA G+PI+ QIN DKNFAF+EFRS++ET G+ DGI + G LK+RRPRDY P FD + S + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+L+ D+EYEEI+EDV++ECSK+G V S++IPRP D V GVGK+FVEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 134 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV------------PRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 496
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978]) HSP 1 Score: 131.339 bits (329), Expect = 3.511e-36 Identity = 69/148 (46.62%), Postives = 101/148 (68.24%), Query Frame = 2
Query: 1007 MQLGEKRIVVQRACLGA-KNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
MQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+L+ D+EYEEI+EDV++ECSK+G V S++IPRP D V GVGK+FVEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 1 MQLGDKQLVVQLACANQQRHNTNLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 143
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978]) HSP 1 Score: 131.339 bits (329), Expect = 3.511e-36 Identity = 69/148 (46.62%), Postives = 101/148 (68.24%), Query Frame = 2
Query: 1007 MQLGEKRIVVQRACLGA-KNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
MQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+L+ D+EYEEI+EDV++ECSK+G V S++IPRP D V GVGK+FVEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 1 MQLGDKQLVVQLACANQQRHNTNLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 143
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307178) HSP 1 Score: 405.601 bits (1041), Expect = 2.311e-138 Identity = 206/392 (52.55%), Postives = 274/392 (69.90%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNV----EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
+K YWDV P G+E+++ ++K + + GQ+ S+ V + TA P +G+T++RQARR+YVGN+P GVTEE MMEFFN+Q+ L L QA G P++A QIN DKNFAFLEFRS++ET Q + DGI G +LK+RRP DY P+P D +VS+ + + +V D+PHK+F+GGLP YL +DQVKELL +FG LR FNLVKD+ TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQRA +GAKNA TQ +M+ +SNV TEVLCL NMV D+LRD+EEYE+I+ED+KEEC+K+G V SV+IPRP + V G GK+FVEF + + KA +AL+GR FS +VVVTS++D +KY F
Sbjct: 38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQNAAN----TTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVE-VPGCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0074234) HSP 1 Score: 405.601 bits (1041), Expect = 2.311e-138 Identity = 206/392 (52.55%), Postives = 274/392 (69.90%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNV----EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
+K YWDV P G+E+++ ++K + + GQ+ S+ V + TA P +G+T++RQARR+YVGN+P GVTEE MMEFFN+Q+ L L QA G P++A QIN DKNFAFLEFRS++ET Q + DGI G +LK+RRP DY P+P D +VS+ + + +V D+PHK+F+GGLP YL +DQVKELL +FG LR FNLVKD+ TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQRA +GAKNA TQ +M+ +SNV TEVLCL NMV D+LRD+EEYE+I+ED+KEEC+K+G V SV+IPRP + V G GK+FVEF + + KA +AL+GR FS +VVVTS++D +KY F
Sbjct: 38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQNAAN----TTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVE-VPGCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307176) HSP 1 Score: 405.601 bits (1041), Expect = 3.974e-138 Identity = 206/392 (52.55%), Postives = 274/392 (69.90%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNV----EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
+K YWDV P G+E+++ ++K + + GQ+ S+ V + TA P +G+T++RQARR+YVGN+P GVTEE MMEFFN+Q+ L L QA G P++A QIN DKNFAFLEFRS++ET Q + DGI G +LK+RRP DY P+P D +VS+ + + +V D+PHK+F+GGLP YL +DQVKELL +FG LR FNLVKD+ TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQRA +GAKNA TQ +M+ +SNV TEVLCL NMV D+LRD+EEYE+I+ED+KEEC+K+G V SV+IPRP + V G GK+FVEF + + KA +AL+GR FS +VVVTS++D +KY F
Sbjct: 49 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQNAAN----TTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVE-VPGCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 427
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307177) HSP 1 Score: 385.185 bits (988), Expect = 3.241e-131 Identity = 198/372 (53.23%), Postives = 261/372 (70.16%), Query Frame = 2
Query: 362 EFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNV----EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
++K + + GQ+ S+ V + TA P +G+T++RQARR+YVGN+P GVTEE MMEFFN+Q+ L L QA G P++A QIN DKNFAFLEFRS++ET Q + DGI G +LK+RRP DY P+P D +VS+ + + +V D+PHK+F+GGLP YL +DQVKELL +FG LR FNLVKD+ TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQRA +GAKNA TQ +M+ +SNV TEVLCL NMV D+LRD+EEYE+I+ED+KEEC+K+G V SV+IPRP + V G GK+FVEF + + KA +AL+GR FS +VVVTS++D +KY F
Sbjct: 2 QYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQNAAN----TTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVE-VPGCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 360
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: LS2 (gene:FBgn0034834 transcript:FBtr0072052) HSP 1 Score: 318.161 bits (814), Expect = 1.137e-103 Identity = 182/398 (45.73%), Postives = 255/398 (64.07%), Query Frame = 2
Query: 320 WDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQI-----KLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQ----AFDGIVSNSMTA--------------GIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEE----TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY 1432
WD+ P GY +++ ++K + + GQ+ +++ G+ E +I + ++RQARR+YVGN+P GVT+E MM+FFN QI + K DG+ ++ Q N +KNFAFLEFRS++E +Q LN DG+V+ G LK+RRP DY P+P A + S + A IV D+P+K++VGGLP L +DQVKELLQ+FG L+G NLV D+ T L+KG+AF EY D SVTD A GL GM LG++R+VVQR+ G KNA G L P ++ IS +++ TE LCL NMV ++L DDEE+E+I D+K+EC+KFG V S++IPRP G G GK+FV+F + ++ KA+KALSGR FSG++V+TS+YD EKY
Sbjct: 62 WDIPPEGYNHLTPQQYKAMLASGQI-------ASRILSDGVHGGESAAI-SMITRQARRLYVGNIPFGVTDEEMMQFFNHQIMALGFEAKSSHYMDGNAVLTCQTNLEKNFAFLEFRSIDEASQALNFDGMVFRGQTLKIRRPHDYQPVPSISVSAMESYRSFRVPAINVAQQPAVTLPVTTIVPDSPNKIYVGGLPTCLNQDQVKELLQSFGELKGLNLVMDTNTNLNKGFAFFEYCDPSVTDHAIAGLHGMLLGDRRLVVQRSIPGGKNAF---PGHL----PTVLQVPGISTLQDPGSPTETLCLLNMVRPEELLDDEEFEDIRTDIKQECAKFGEVRSIKIPRPIGQFPK-RGCGKVFVQFESVEDSQKALKALSGRKFSGRIVMTSYYDPEKY 443
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881]) HSP 1 Score: 400.208 bits (1027), Expect = 3.007e-135 Identity = 203/393 (51.65%), Postives = 270/393 (68.70%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQ------------AFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQP-MMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ +LK+RRP DY PLP F G+VS +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +V D+A GL GMQL +K+++VQRA +GAKNA + I P+ L P + +P TEVLCL NMV ++L DDEEYEEI+EDVKEECSK+G V S++IPRP D + G GKIFVEF + ++ KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 94 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQAQSLKIRRPHDYQPLPGMSENPSVYVPAVQFQGVVST-----VVPDSIHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNVNDQAIAGLNGMQLADKKLLVQRASVGAKNATMTSINETPVTLQVPGLTSNPMIQMGGIPTEVLCLMNMVAPEELIDDEEYEEIVEDVKEECSKYGQVKSIEIPRPVDGLD-IPGTGKIFVEFTSVYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 480
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881]) HSP 1 Score: 399.823 bits (1026), Expect = 4.167e-135 Identity = 199/383 (51.96%), Postives = 269/383 (70.23%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQP-MMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +V D+A GL GMQL +K+++VQRA +GAKNA + I P+ L P + +P TEVLCL NMV ++L DDEEYEEI+EDVKEECSK+G V S++IPRP D + G GKIFVEF + ++ KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 94 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQAQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSIHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNVNDQAIAGLNGMQLADKKLLVQRASVGAKNATMTSINETPVTLQVPGLTSNPMIQMGGIPTEVLCLMNMVAPEELIDDEEYEEIVEDVKEECSKYGQVKSIEIPRPVDGLD-IPGTGKIFVEFTSVYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 475
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131]) HSP 1 Score: 382.874 bits (982), Expect = 1.198e-128 Identity = 199/387 (51.42%), Postives = 276/387 (71.32%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEE-----TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +++D+A GL GMQLG+K+++VQRA +G+KN +GI P+ L P +M+ S+V + TEVLCL NMV ++L DDEEYEEI+EDV++ECSK+G V S++IPRP D + G GKIFVEF + ++ KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 84 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPEGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTTLTGINQTPVTLQVPGLMN----SSVNQMGGIPTEVLCLMNMVAPEELLDDEEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLD-IPGTGKIFVEFMSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 465
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131]) HSP 1 Score: 382.874 bits (982), Expect = 1.332e-128 Identity = 202/392 (51.53%), Postives = 277/392 (70.66%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDG---IVSNSMTAGIVQ----DTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEE-----TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + V S+ G+V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +++D+A GL GMQLG+K+++VQRA +G+KN +GI P+ L P +M+ S+V + TEVLCL NMV ++L DDEEYEEI+EDV++ECSK+G V S++IPRP D + G GKIFVEF + ++ KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 84 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPEGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGSLPVGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTTLTGINQTPVTLQVPGLMN----SSVNQMGGIPTEVLCLMNMVAPEELLDDEEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLD-IPGTGKIFVEFMSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 470
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881]) HSP 1 Score: 220.705 bits (561), Expect = 1.497e-68 Identity = 115/213 (53.99%), Postives = 148/213 (69.48%), Query Frame = 2
Query: 698 VYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQP-MMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKI 1321
++ +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +V D+A GL GMQL +K+++VQRA +GAKNA + I P+ L P + +P TEVLCL NMV ++L DDEEYEEI+EDVKEECSK+G V S++IPRP D + G GK+
Sbjct: 2 IFQAQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSIHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNVNDQAIAGLNGMQLADKKLLVQRASVGAKNATMTSINETPVTLQVPGLTSNPMIQMGGIPTEVLCLMNMVAPEELIDDEEYEEIVEDVKEECSKYGQVKSIEIPRPVDGLD-IPGTGKV 213
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616]) HSP 1 Score: 407.527 bits (1046), Expect = 8.133e-139 Identity = 202/387 (52.20%), Postives = 273/387 (70.54%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DD+EYEEI+EDV++EC K+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 34 KKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDDEYEEIVEDVRDECGKYGAVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDGYHRRDFW 419
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616]) HSP 1 Score: 407.912 bits (1047), Expect = 2.443e-138 Identity = 202/387 (52.20%), Postives = 273/387 (70.54%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DD+EYEEI+EDV++EC K+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 71 KKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDDEYEEIVEDVRDECGKYGAVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDGYHRRDFW 456
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616]) HSP 1 Score: 404.06 bits (1037), Expect = 1.207e-136 Identity = 204/398 (51.26%), Postives = 274/398 (68.84%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNS-------------MTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP G+ N + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DD+EYEEI+EDV++EC K+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 77 KKIRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLP----GMSENPSVYVPGRNLPQLWVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDDEYEEIVEDVRDECGKYGAVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDGYHRRDFW 469
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: puf60 (poly(U) binding splicing factor 60KDa [Source:NCBI gene;Acc:100145091]) HSP 1 Score: 76.2554 bits (186), Expect = 2.893e-14 Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2
Query: 1124 KISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQI-PRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRN 1438
K+ +E+ V+ L NMV D+ DD E E V EEC KFG V V I G +++ + KIFVEF SET KA++AL+GR F+G+ VV YD E++ N
Sbjct: 356 KLMRKQESTVMVLRNMVDPRDIDDDLEGE-----VTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMSSETQKAIQALNGRWFAGRKVVAEVYDQERFDN 456
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: puf60 (poly(U) binding splicing factor 60KDa [Source:NCBI gene;Acc:100145091]) HSP 1 Score: 75.8702 bits (185), Expect = 4.720e-14 Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2
Query: 1124 KISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQI-PRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRN 1438
K+ +E+ V+ L NMV D+ DD E E V EEC KFG V V I G +++ + KIFVEF SET KA++AL+GR F+G+ VV YD E++ N
Sbjct: 415 KLMRKQESTVMVLRNMVDPRDIDDDLEGE-----VTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMSSETQKAIQALNGRWFAGRKVVAEVYDQERFDN 515
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886]) HSP 1 Score: 390.578 bits (1002), Expect = 1.769e-131 Identity = 204/387 (52.71%), Postives = 274/387 (70.80%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 471
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886]) HSP 1 Score: 389.423 bits (999), Expect = 5.574e-131 Identity = 204/391 (52.17%), Postives = 274/391 (70.08%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA------SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886]) HSP 1 Score: 318.546 bits (815), Expect = 1.089e-105 Identity = 173/308 (56.17%), Postives = 223/308 (72.40%), Query Frame = 2
Query: 539 MMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 1 MMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 307
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: Uhmk1 (U2AF homology motif (UHM) kinase 1 [Source:MGI Symbol;Acc:MGI:1341908]) HSP 1 Score: 86.6557 bits (213), Expect = 3.602e-18 Identity = 43/101 (42.57%), Postives = 65/101 (64.36%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
T VL L N++ D L +++EYE+++EDVKEEC K+G V+S+ +P+ G G++FVE+ N ++ A K L+GR F GK VV +FY L Y+ G+
Sbjct: 231 TPVLRLLNVLDDDYLENEDEYEDVVEDVKEECQKYGPVVSLLVPKEN------PGRGQVFVEYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKRGYL 325
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: Uhmk1 (U2AF homology motif (UHM) kinase 1 [Source:MGI Symbol;Acc:MGI:1341908]) HSP 1 Score: 87.0409 bits (214), Expect = 6.144e-18 Identity = 43/101 (42.57%), Postives = 65/101 (64.36%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
T VL L N++ D L +++EYE+++EDVKEEC K+G V+S+ +P+ G G++FVE+ N ++ A K L+GR F GK VV +FY L Y+ G+
Sbjct: 320 TPVLRLLNVLDDDYLENEDEYEDVVEDVKEECQKYGPVVSLLVPKEN------PGRGQVFVEYANAGDSKAAQKLLTGRMFDGKFVVATFYPLSAYKRGYL 414
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|Q24562|U2AF2_DROME (Splicing factor U2AF 50 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af50 PE=1 SV=1) HSP 1 Score: 405.601 bits (1041), Expect = 2.316e-137 Identity = 206/392 (52.55%), Postives = 274/392 (69.90%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNV----EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
+K YWDV P G+E+++ ++K + + GQ+ S+ V + TA P +G+T++RQARR+YVGN+P GVTEE MMEFFN+Q+ L L QA G P++A QIN DKNFAFLEFRS++ET Q + DGI G +LK+RRP DY P+P D +VS+ + + +V D+PHK+F+GGLP YL +DQVKELL +FG LR FNLVKD+ TGLSKGYAFCEY D S+TD++ GL GMQLG+K+++VQRA +GAKNA TQ +M+ +SNV TEVLCL NMV D+LRD+EEYE+I+ED+KEEC+K+G V SV+IPRP + V G GK+FVEF + + KA +AL+GR FS +VVVTS++D +KY F
Sbjct: 38 RKPSLYWDVPPPGFEHITPMQYKAMQASGQIPASV------VPDTPQTA--VPVVGSTITRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGSPVLACQINLDKNFAFLEFRSIDETTQAMAFDGINLKGQSLKIRRPHDYQPMPGITDTPAIKPAVVSSGVISTVVPDSPHKIFIGGLPNYLNDDQVKELLLSFGKLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQNAAN----TTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVE-VPGCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P26368|U2AF2_HUMAN (Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4) HSP 1 Score: 389.808 bits (1000), Expect = 3.072e-130 Identity = 204/391 (52.17%), Postives = 274/391 (70.08%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA------SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P26369|U2AF2_MOUSE (Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3) HSP 1 Score: 389.423 bits (999), Expect = 3.899e-130 Identity = 204/391 (52.17%), Postives = 274/391 (70.08%), Query Frame = 2
Query: 302 KKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA------SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
KK KYWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +VTD+A GL GMQLG+K+++VQRA +GAKNA S I P+ L P +M TEVLCL NMV+ ++L DDEEYEEI+EDV++ECSK+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 86 KKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVE-VPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P90727|U2AF2_CAEBR (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis briggsae OX=6238 GN=uaf-1 PE=3 SV=2) HSP 1 Score: 362.073 bits (928), Expect = 2.288e-119 Identity = 187/382 (48.95%), Postives = 255/382 (66.75%), Query Frame = 2
Query: 305 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLG-AKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
K Y++WDV PTG+EN++ E+K + + G V G S P +G +V+ Q+RR+YVGN+P G EE M++FFN+Q+ L L QA G+PI+ QIN DKNFAF+EFRS++ET G+ DGI + G LK+RRPRDY P FD + + + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+LR DE+YEEI+EDV+EECSK+G V S++IPRP D V GVGK+FVEF S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 125 KKYRFWDVPPTGFENITPMEYKNMQASG------------AVPRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCNLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNARMPVSSIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQTRHNTHLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELRSDEDYEEILEDVREECSKYGIVRSLEIPRPYD-DHPVPGVGKVFVEFATTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 487
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P90978|U2AF2_CAEEL (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 PE=2 SV=2) HSP 1 Score: 358.221 bits (918), Expect = 1.016e-117 Identity = 186/382 (48.69%), Postives = 255/382 (66.75%), Query Frame = 2
Query: 305 KNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLG-AKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
K Y++WDV PTG+E + E+K + + GQV G S P +G +V+ Q+RR+YVGN+P G EE M++FFN+Q+ L L QA G+PI+ QIN DKNFAF+EFRS++ET G+ DGI + G LK+RRPRDY P FD + S + IV D+ +K+F+GGLP YL EDQVKELL +FGPL+ F+L DS G SKGYAF EY D ++TD+A GL GMQLG+K++VVQ AC ++ + +P + +D ++ + TE+LCL NMV D+L+ D+EYEEI+EDV++ECSK+G V S++IPRP D V GVGK+FVEF + S+ +A AL+GR F+ + VVTS+YD++KY N F
Sbjct: 134 KKYRFWDVPPTGFETTTPMEYKNMQAAGQV------------PRGSVQSAVPVVGPSVTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPGNPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDYQPSQNTFD-MNSRMPVSTIVVDSANKIFIGGLPNYLTEDQVKELLCSFGPLKAFSLNVDS-QGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQRHNTNLPNSASAIAG---IDLSQGAG-RATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY-EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 496
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0R3X1A4 (U2 snRNP auxiliary factor large subunit OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS6952 PE=3 SV=1) HSP 1 Score: 427.172 bits (1097), Expect = 5.662e-142 Identity = 219/397 (55.16%), Postives = 279/397 (70.28%), Query Frame = 2
Query: 311 YKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLT-----EGNVVNMGLTA-SEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLM----LTQPMMMDPTKISNV------EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YKYWDV P G+E+M+ ++K + + GQ+ ++Y + N LT + P G+ V RQARR+YVGN+P TEE MM+FFN+Q+K + L QA+G+PIIAVQIN +KNFAFLEFRSV+ET QGL LDGI++H ALKLRRPRDY PLP + I+ + + +VQD+PHK+FVGGLP YL EDQVKELL +FGPL+GFNLVKD +TGLSKGYAFCEY D +VTD AC GL GMQLG+K+++VQRA +GAKNA G PL+ L ++ + T N+ TEVLCL NMV +DL +DEEYE+I+EDV+ ECSK+G V SV+IPRP + V GVGKIFV+F + + KA AL+GR F ++VVTSFYD KY F
Sbjct: 110 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEENMMDFFNKQMKAQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGILFHNQALKLRRPRDYAPLPGVSETPSIIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLSFGPLKGFNLVKDGSTGLSKGYAFCEYVDPNVTDHACAGLNGMQLGDKKLIVQRASVGAKNAGGAMPPPLIQVPGLNDALVQNTTGSGNIIFRSGGPPTEVLCLLNMVDPEDLTNDEEYEDIVEDVRAECSKYGIVSSVEIPRPIPGVE-VPGVGKIFVQFASLVDCQKAATALTGRKFDNRLVVTSFYDPNKYHRREF 505
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A068Y9C9 (U2 snRNP auxiliary factor large subunit OS=Echinococcus multilocularis OX=6211 PE=3 SV=1) HSP 1 Score: 426.787 bits (1096), Expect = 6.043e-142 Identity = 219/397 (55.16%), Postives = 279/397 (70.28%), Query Frame = 2
Query: 311 YKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLT-----EGNVVNMGLTA-SEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLM----LTQPMMMDPTKISNV------EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YKYWDV P G+E+M+ ++K + + GQ+ ++Y + N LT + P G+ V RQARR+YVGN+P TEE MM+FFN+Q+K + L QA+G+PIIAVQIN +KNFAFLEFRSV+ET QGL LDGI++H ALKLRRPRDY PLP + I+ + + +VQD+PHK+FVGGLP YL EDQVKELL +FGPL+GFNLVKD +TGLSKGYAFCEY D +VTD AC GL GMQLG+K+++VQRA +GAKNA G PL+ L ++ + T N+ TEVLCL NMV +DL +DEEYE+I+EDV+ ECSK+G V SV+IPRP + V GVGKIFV+F + + KA AL+GR F ++VVTSFYD KY F
Sbjct: 110 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEENMMDFFNKQMKAQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGILFHNQALKLRRPRDYAPLPGVSETPSIIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLSFGPLKGFNLVKDGSTGLSKGYAFCEYVDPNVTDHACAGLNGMQLGDKKLIVQRASVGAKNAGGAMPPPLIQVPGLNDALVQNTTGSGNIIFRSGGPPTEVLCLLNMVDPEDLTNDEEYEDIVEDVRAECSKYGIVSSVEIPRPIPGVE-VPGVGKIFVQFASLVDCQKAATALTGRKFDNRLVVTSFYDPNKYHRREF 505
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: U6J590 (U2 snRNP auxiliary factor large subunit OS=Echinococcus granulosus OX=6210 GN=EGR_02775 PE=3 SV=1) HSP 1 Score: 426.787 bits (1096), Expect = 6.043e-142 Identity = 219/397 (55.16%), Postives = 279/397 (70.28%), Query Frame = 2
Query: 311 YKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLT-----EGNVVNMGLTA-SEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLM----LTQPMMMDPTKISNV------EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YKYWDV P G+E+M+ ++K + + GQ+ ++Y + N LT + P G+ V RQARR+YVGN+P TEE MM+FFN+Q+K + L QA+G+PIIAVQIN +KNFAFLEFRSV+ET QGL LDGI++H ALKLRRPRDY PLP + I+ + + +VQD+PHK+FVGGLP YL EDQVKELL +FGPL+GFNLVKD +TGLSKGYAFCEY D +VTD AC GL GMQLG+K+++VQRA +GAKNA G PL+ L ++ + T N+ TEVLCL NMV +DL +DEEYE+I+EDV+ ECSK+G V SV+IPRP + V GVGKIFV+F + + KA AL+GR F ++VVTSFYD KY F
Sbjct: 110 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEENMMDFFNKQMKAQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGILFHNQALKLRRPRDYAPLPGVSETPSIIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLSFGPLKGFNLVKDGSTGLSKGYAFCEYVDPNVTDHACAGLNGMQLGDKKLIVQRASVGAKNAGGAMPPPLIQVPGLNDALVQNTTGSGNIIFRSGGPPTEVLCLLNMVDPEDLTNDEEYEDIVEDVRAECSKYGIVSSVEIPRPIPGVE-VPGVGKIFVQFASLVDCQKAATALTGRKFDNRLVVTSFYDPNKYHRREF 505
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0R3TPX5 (U2 snRNP auxiliary factor large subunit OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS9552 PE=3 SV=1) HSP 1 Score: 426.402 bits (1095), Expect = 8.371e-142 Identity = 218/397 (54.91%), Postives = 280/397 (70.53%), Query Frame = 2
Query: 311 YKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLT-----EGNVVNMGLTA-SEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLM----LTQPMMMDPTKISNV------EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YKYWDV P G+E+M+ ++K + + GQ+ ++Y + N LT + P G+ V RQARR+YVGN+P TE+ MM+FFN+Q+K + L QA+G+PIIAVQIN +KNFAFLEFRSV+ET QGL LDGI++H ALKLRRPRDY PLP + ++ + + +VQD+PHK+FVGGLP YL EDQVKELL +FGPL+GFNLVKD +TGLSKGYAFCEY D +VTD AC GL GMQLG+K+++VQRA +GAKN+SG PL+ L ++ + T N+ TEVLCL NMV +DL +DEEYE+I+EDV+ ECSK+G V SV+IPRP D V GVGKIFV+F + + KA AL+GR F ++VVTSFYD KY F
Sbjct: 108 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEDNMMDFFNKQMKAQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGILFHDQALKLRRPRDYAPLPGVSETPSVIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLSFGPLKGFNLVKDGSTGLSKGYAFCEYVDPNVTDHACAGLNGMQLGDKKLIVQRASVGAKNSSGTLPLPLIQVPGLNDALVQNTTGSGNIILRSGGPPTEVLCLLNMVDPEDLTNDEEYEDIVEDVRAECSKYGIVSSVEIPRPIPGVD-VPGVGKIFVQFASLVDCQKAATALTGRKFDNRLVVTSFYDPNKYHRREF 503
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0R3SJW2 (U2 snRNP auxiliary factor large subunit OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS5225 PE=3 SV=1) HSP 1 Score: 426.402 bits (1095), Expect = 8.554e-142 Identity = 218/397 (54.91%), Postives = 280/397 (70.53%), Query Frame = 2
Query: 311 YKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLT-----EGNVVNMGLTA-SEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLM----LTQPMMMDPTKISNV------EETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YKYWDV P G+E+M+ ++K + + GQ+ ++Y + N LT + P G+ V RQARR+YVGN+P TE+ MM+FFN+Q+K + L QA+G+PIIAVQIN +KNFAFLEFRSV+ET QGL LDGI++H ALKLRRPRDY PLP + ++ + + +VQD+PHK+FVGGLP YL EDQVKELL +FGPL+GFNLVKD +TGLSKGYAFCEY D +VTD AC GL GMQLG+K+++VQRA +GAKN+SG PL+ L ++ + T N+ TEVLCL NMV +DL +DEEYE+I+EDV+ ECSK+G V SV+IPRP D V GVGKIFV+F + + KA AL+GR F ++VVTSFYD KY F
Sbjct: 110 YKYWDVPPPGFEHMTPQQYKALQASGQIPVNVYAAGQVPMPASTPNAPLTLNTNIPFAGSAVCRQARRLYVGNIPFTATEDNMMDFFNKQMKSQNLVQAEGNPIIAVQINMEKNFAFLEFRSVDETTQGLALDGILFHDQALKLRRPRDYAPLPGVSETPSVIVPGVVSTVVQDSPHKIFVGGLPNYLNEDQVKELLLSFGPLKGFNLVKDGSTGLSKGYAFCEYVDPNVTDHACAGLNGMQLGDKKLIVQRASVGAKNSSGTLPPPLIQVPGLNDALVQNTTGSGNIILRSGGPPTEVLCLLNMVDPEDLTNDEEYEDIVEDVRAECSKYGIVSSVEIPRPIPGVD-VPGVGKIFVQFASLVDCQKAATALTGRKFDNRLVVTSFYDPNKYHRREF 505
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2a (splicing factor U2AF 65 kDa subunit-like [Source:NCBI gene;Acc:103024792]) HSP 1 Score: 400.979 bits (1029), Expect = 9.302e-136 Identity = 199/392 (50.77%), Postives = 277/392 (70.66%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDG--------IVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTG----PLMLTQPMMMDPT--KISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + V + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D +++D+A GL GMQLG+K+++VQRA +G+KN S + TG P+ L P +M+ + ++ + TEVLCL NMV ++L DD+EYEEI+EDV+EECSK+G V S++IPRP + V G GKIFVEF + ++ KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 81 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPEGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGLSVPPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTSLVGTGMNQTPVTLQVPGLMNSSMNQMGGI-PTEVLCLMNMVSPEELLDDDEYEEIVEDVREECSKYGQVKSIEIPRPVDGLE-VPGTGKIFVEFMSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 470
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2b (splicing factor U2AF 65 kDa subunit [Source:NCBI gene;Acc:103026862]) HSP 1 Score: 400.593 bits (1028), Expect = 1.047e-135 Identity = 197/383 (51.44%), Postives = 272/383 (71.02%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVE--ETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D ++ D+A GL GMQLG+K+++VQRA +GAKNA + I P+ L P +M+ T ++ + TEVLCL NMV ++L DD+EYEEI+EDV++ECSK+G V S++IPRP D + G GKIFVEF ++ KAM+ L+GR F+ +VVVT + D + Y F
Sbjct: 75 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPEGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSVHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGAKNATMTSINETPVTLQVPGLMN-TSMNQMGGIPTEVLCLMNMVAPEELLDDDEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLD-IPGTGKIFVEFTTLYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDF 455
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2b (splicing factor U2AF 65 kDa subunit [Source:NCBI gene;Acc:103026862]) HSP 1 Score: 400.208 bits (1027), Expect = 1.843e-135 Identity = 197/383 (51.44%), Postives = 272/383 (71.02%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVE--ETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D ++ D+A GL GMQLG+K+++VQRA +GAKNA + I P+ L P +M+ T ++ + TEVLCL NMV ++L DD+EYEEI+EDV++ECSK+G V S++IPRP D + G GKIFVEF ++ KAM+ L+GR F+ +VVVT + D + Y F
Sbjct: 82 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPEGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSVHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGAKNATMTSINETPVTLQVPGLMN-TSMNQMGGIPTEVLCLMNMVAPEELLDDDEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLD-IPGTGKIFVEFTTLYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDF 462
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: uhmk1 (U2AF homology motif kinase 1 [Source:NCBI gene;Acc:103036001]) HSP 1 Score: 91.2781 bits (225), Expect = 1.911e-19 Identity = 44/101 (43.56%), Postives = 67/101 (66.34%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
T VL L N++ L +D+EYEEI+ED++EEC K+G+V+S+ IP+ G G++FVE+ N ++ +A + L+GRTF GK VV +FY L Y+ G+
Sbjct: 332 TPVLRLLNVIDDCHLHNDDEYEEIIEDMREECQKYGSVISLLIPKEN------PGKGQVFVEYANAGDSKEAQRLLTGRTFDGKFVVATFYPLSAYKRGYL 426
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: uhmk1 (U2AF homology motif kinase 1 [Source:NCBI gene;Acc:103036001]) HSP 1 Score: 91.2781 bits (225), Expect = 2.073e-19 Identity = 44/101 (43.56%), Postives = 67/101 (66.34%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
T VL L N++ L +D+EYEEI+ED++EEC K+G+V+S+ IP+ G G++FVE+ N ++ +A + L+GRTF GK VV +FY L Y+ G+
Sbjct: 332 TPVLRLLNVIDDCHLHNDDEYEEIIEDMREECQKYGSVISLLIPKEN------PGKGQVFVEYANAGDSKEAQRLLTGRTFDGKFVVATFYPLSAYKRGYL 426
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131]) HSP 1 Score: 398.282 bits (1022), Expect = 1.689e-135 Identity = 205/381 (53.81%), Postives = 269/381 (70.60%), Query Frame = 2
Query: 320 WDVAPTGYENMSAAEFKQIHSGGQV-LPSLYLTEGNVVNMGL---TASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSN---SMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASG--IPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYR 1435
WDV P G+E+++ ++K + + GQ+ +L T GL A P +G+ ++RQARR+YVGN+P GVTEE MM+FFN Q+KL L QA G+P++AVQIN DKNFAFLEFRSV+ET Q L DGI++ G +LKLRRP DY PLP + + + + +VQDTPHKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS T SKGYAFCEYAD +VTD+A GL GMQLG+K+++VQRA +GAKNA+ + P+ L P + + TEVLCL NMV ++L DDEEYE+I+ED++EEC K+G+V SV+IPRP D + G GKIFVEF + + +A +ALSGR F+ +VVVT +Y+LEKY
Sbjct: 77 WDVPPPGFEHITPLQYKAMQAAGQIPATALIATTTTATVAGLPNPAAMPVPIVGSQLTRQARRLYVGNIPFGVTEEAMMDFFNNQMKLAGLAQAPGNPVLAVQINLDKNFAFLEFRSVDETTQALAFDGIIFQGQSLKLRRPHDYQPLPGMSESPALHVPVGVVSTVVQDTPHKLFIGGLPSYLTDDQVKELLTSFGPLKAFNLVKDSATCFSKGYAFCEYADVNVTDQAIAGLNGMQLGDKKLIVQRASVGAKNANPTVVVQTPVTLQVPGLASSQVQHSGLPTEVLCLMNMVTPEELVDDEEYEDILEDIREECGKYGSVRSVEIPRPVNGLD-IPGCGKIFVEFASLLDCQRAQQALSGRKFANRVVVTQYYNLEKYH 456
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: RBM39 (RNA binding motif protein 39 [Source:HGNC Symbol;Acc:HGNC:15923]) HSP 1 Score: 76.2554 bits (186), Expect = 4.061e-15 Identity = 91/375 (24.27%), Postives = 149/375 (39.73%), Query Frame = 2
Query: 482 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRP---RDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVV-------------------------------QRACLGAKNASG----IP---------TGPLML--TQPMM-----MDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGF 1444
R AR ++ L + + EFF+ K++++ I K A++EF V+ + + L G G + ++ ++ A + S TAG P +L+VG L + ED +K + + FG + L+ DS TG S+GY F +AD KA L G +L + + V R L AK A G IP TG L T P+M P I+ T+ + L NM + + ++I +DV EC K G ++ + + D S G ++V+ + + + + AL GR F+GK++ +F + Y N F
Sbjct: 156 RDARTVFCMQLAARIRPRDLEEFFSAVGKVRDVRM-----ISDRNSRRSKGIAYIEFVEVDSVSLAIGLTGQRLLGVPIIVQASQAEKNRAAAAAAAAATMQKS-TAG-----PMRLYVGSLHFNITEDMLKGIFEPFGKIENCQLMMDSDTGRSRGYGFVTFADAECARKALEQLNGFELAGRPMKVGHVTERSDGSGASSFLDNDELERTGIDLGSTGRLHLMAKLAEGTGLQIPLAAQQALQMTGGFGLGTTNPVMPVLNNAAPPSIA----TQCIQLSNMFSPESEQSHTWDQDIRDDVITECMKHGGLVHIFV-------DKNSPQGNVYVKCSSVASAIATVNALHGRWFAGKMITAAFVPVPTYHNLF 508
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: RBM39 (RNA binding motif protein 39 [Source:HGNC Symbol;Acc:HGNC:15923]) HSP 1 Score: 66.2402 bits (160), Expect = 6.463e-12 Identity = 86/391 (21.99%), Postives = 148/391 (37.85%), Query Frame = 2
Query: 482 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKN------FAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGI--VQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQ--------------------------------------RACLGAKNASG------------------IPTGPLM----LTQPMM-MDPT-KISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGF 1444
R +R ++ L + + + EFF+ K+++ V++ +D+N A++EF ++ + L G G + V QA + + A + + P +L+VG L + E + + + FG + L+ D TG SKG+ F ++ KA L G +L + + V R L AK A G IP LM P++ M+PT +S T+ L NM + +EI +DV EEC+K G V+ + + D S G ++V+ N + A+ +L GR F+GK++ ++ + Y N F
Sbjct: 171 RDSRTVFCMQLAARIRAKDLEEFFSAVGKVRD-----------VRLISDRNSRRSKGIAYIEFVDLDSVPLAIGLTGQRLLGVPI-------IVQASQAEKNRAAAAAAANLQKLASGPMRLYVGSLHFNITEAMLMGIFEPFGKIDNIQLMMDPETGRSKGFGFITFSSADCAKKALEQLNGFELAGRPMKVNYVSDHSDVIISVGSGGGGSSFLDNDELERTGIDLTNTGRLQLMAKLAEGSGLTIPPVAQQALQMNGAIPLEALMPSLAACGPLVAMNPTFTMSPTIATQCFQLSNMFNPQSEVNSSWVQEIRDDVVEECNKHGGVVHIYV-------DQNSAEGHVYVKCPNIPVALAAVNSLHGRWFAGKMITAAYVPVPTYHNLF 536
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: rbm34 (RNA binding motif protein 34 [Source:ZFIN;Acc:ZDB-GENE-040718-77]) HSP 1 Score: 62.3882 bits (150), Expect = 7.371e-11 Identity = 49/224 (21.88%), Postives = 105/224 (46.88%), Query Frame = 2
Query: 482 RQARRIYVGNLPLGVTEEGMMEFFN-----EQIKLKELTQADGDPII---AVQINT-----DKNFAFLEFRSVEETNQGLNLDGI-VYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMG--LTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQP 1105
++ R ++VGNLP+ + +E + +FF E ++L+ + +AD + + A+Q T +KN A++ F+ + Q L+ +G + GF +++ ++ + D +F+G LP + ED+++ + GP+ +V+D +G+ KG+ + + TD + L G ++ +++ V+R+ K G P ++P
Sbjct: 107 KRKRTVFVGNLPMSLAKEAVKKFFRPCGVIESVRLRCIARADPEMSLKQAAIQGKTHPQRNNKN-AYVVFKEEQGAVQALSRNGEEIQSGFHIRV------------------DAASGEKNHDHKRSVFIGNLPYDVEEDRIRSHFEPCGPVESVRVVRDRESGMGKGFGYILFKG---TDAVLLALHLDGSEMAGRKVRVKRSVKKEKIKPGRAGRPANSSRP 308
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: HNRNPA2B1 (heterogeneous nuclear ribonucleoprotein A2/B1 [Source:HGNC Symbol;Acc:HGNC:5033]) HSP 1 Score: 59.6918 bits (143), Expect = 2.329e-10 Identity = 41/167 (24.55%), Postives = 72/167 (43.11%), Query Frame = 2
Query: 485 QARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDK 985
Q R++++G L TEE + E++ + + + DP + F F+ + +EE N + G ++ +R VP + GI Q T KLFVGG+ E +++ FG + ++V D TG +G+AF + D+ D+
Sbjct: 12 QMRKLFIGGLSFETTEESLREYYEQWGTIVDCVVMK-DP----SSKRSRGFGFITYSVMEEVNSAMQARPHTVDGRVVEAKRA---VPRDDSRPGI----------QVTVKKLFVGGIKDNTDESHLRDYFSKFGRVESVDMVTDKQTGKKRGFAFVTFDDYDPVDQ 160
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Match: RNA15 (Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability [Source:SGD;Acc:S000003012]) HSP 1 Score: 54.6842 bits (130), Expect = 9.697e-9 Identity = 27/81 (33.33%), Postives = 44/81 (54.32%), Query Frame = 2
Query: 785 MTAGIVQDTPHKL-FVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEK 1024
+ AG+ + P ++ ++G +P E+Q+ +L GP+ ++ D TG SKGYAF E+ D + A L G QLG +
Sbjct: 7 VNAGVQNNPPSRVVYLGSIPYDQTEEQILDLCSNVGPVINLKMMFDPQTGRSKGYAFIEFRDLESSASAVRNLNGYQLGSR 87
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Match: NOP13 (Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress [Source:SGD;Acc:S000005119]) HSP 1 Score: 53.1434 bits (126), Expect = 5.003e-8 Identity = 47/203 (23.15%), Postives = 92/203 (45.32%), Query Frame = 2
Query: 497 IYVGNLPLGVTEEGMMEFF------NEQIKLK----ELTQADGDPIIAVQINTDKN--FAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHK-LFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYAD-----HSVTDKACMGLTG----MQLGEKRIVVQ 1039
+++GNL T++ ++ FF NE K + ++T+ + A N KN F ++ F++VE+ L L +G + ++ +Y P D + + ++ P + LFVG L + +D +++ Q G + + +G KG+AF ++ + +++ DK+C + G M+ GE R Q
Sbjct: 127 VWIGNLSFDTTKDDLVRFFIAKTKDNEDEKSRVTEQDITRLSMPRVAAKNSNAMKNKGFCYMFFKNVEQMKAVLELSESHLNGRNMLIKDSENYSGRPDKDDLVA-------MSKNPPSRILFVGNLSFDVTDDLLRKHFQHCGDIVKIRMATFEDSGKCKGFAFIDFKNEEGSTNALKDKSCRKIAGRPLRMEYGEDRSKRQ 322
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Match: PUB1 (Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004961]) HSP 1 Score: 50.0618 bits (118), Expect = 5.559e-7 Identity = 47/192 (24.48%), Postives = 79/192 (41.15%), Query Frame = 2
Query: 491 RRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKN-----FAFLEFRSVEETNQGLN-LDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRAC 1048
R +YVGNL +TE+ + ++F Q+ G PI ++I DKN +AF+E+ + N L L+G +K+ AF S+S D LFVG L + ++ ++ + F +++ D TG S+GY F + A + G L + + + A
Sbjct: 75 RVLYVGNLDKAITEDILKQYF--QV---------GGPIANIKIMIDKNNKNVNYAFVEYHQSHDANIALQTLNGKQIENNIVKIN---------WAFQSQQSSS-------DDTFNLFVGDLNVNVDDETLRNAFKDFPSYLSGHVMWDMQTGSSRGYGFVSFTSQDDAQNAMDSMQGQDLNGRPLRINWAA 239
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Match: HRP1 (Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability [Source:SGD;Acc:S000005483]) HSP 1 Score: 49.2914 bits (116), Expect = 1.023e-6 Identity = 42/192 (21.88%), Postives = 81/192 (42.19%), Query Frame = 2
Query: 476 VSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRR--PRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRA 1045
+S+++ ++++G L TE+ + E+F + + +L + DP + F FL F ++ + I+ G + +R PRD QD K+FVGG+ + + +E +G + L+ D TG S+G+ F Y D+ C + +++I ++RA
Sbjct: 154 LSKESCKMFIGGLNWDTTEDNLREYFGKYGTVTDL-KIMKDPATG----RSRGFGFLSFEKPSSVDEVVKTQHIL-DGKVIDPKRAIPRD--------------------EQDKTGKIFVGGIGPDVRPKEFEEFFSQWGTIIDAQLMLDKDTGQSRGFGFVTYDSADAVDRVCQNKF-IDFKDRKIEIKRA 318
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Match: PAB1 (Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex [Source:SGD;Acc:S000000967]) HSP 1 Score: 48.521 bits (114), Expect = 1.949e-6 Identity = 65/278 (23.38%), Postives = 116/278 (41.73%), Query Frame = 2
Query: 602 IIAVQINTDKN-----FAFLEFRSVEETNQGLN-LDGIVYHGFALKL-----RRPRDYVPLPQAFDGIVSNSMTAGIVQDTPH--KLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGK 1396
I++ +I TD+N F F+ F + ++ L+G++ +G + + R+ RD + + + H L+V + ++Q +EL FGP+ +L KD+ L KG+ F Y H KA L +L +++ V RA KN M + ++ + + + + L + +DD DDE+ E EE + +GT+ S ++ R +N G FV F E KA+ + + +GK
Sbjct: 152 ILSSKIATDENGKSKGFGFVHFEEEGAAKEAIDALNGMLLNGQEIYVAPHLSRKERD-----------------SQLEETKAHYTNLYVKNINSETTDEQFQELFAKFGPIVSASLEKDADGKL-KGFGFVNYEKHEDAVKAVEALNDSELNGEKLYVGRAQ--KKNER-------MHVLKKQYEAYRLEKMAKYQGVNLF-VKNLDDSVDDEKLE-------EEFAPYGTITSAKVMR----TENGKSKGFGFVCFSTPEEATKAITEKNQQIVAGK 390
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO48329 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJT7]) HSP 1 Score: 347.051 bits (889), Expect = 6.018e-117 Identity = 181/335 (54.03%), Postives = 230/335 (68.66%), Query Frame = 2
Query: 476 VSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNS------MTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKIS-----NVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
++RQARR+YVGN+P GVTE M+EFFN ++K +L A G+P+IA QINT++NFAF+E RSVEET Q + DGI+ G ALK+RRP+DY P+P G+ N+ + + +V D+PHK+F+GGLP YL EDQVKELL +FG LR FNLVKDS TGLSKGYAFCEY D +TD A GL GMQLG+K+++VQRA +GAK P M+ + + IS V TEVL L NMV D+L DDEE+EEI +DV+EECSK+G V S++IPRP GVGKIFVEF + + KA AL GR F+ +VVVTS+Y E+Y F
Sbjct: 1 MTRQARRLYVGNIPFGVTENLMIEFFNAKMKEAKLNTAPGNPVIAAQINTEQNFAFIELRSVEETTQAMAFDGIILQGQALKIRRPKDYQPIP----GMSENASVHVPGVVSTVVPDSPHKIFIGGLPNYLNEDQVKELLSSFGELRAFNLVKDSATGLSKGYAFCEYVDLGITDVAIQGLNGMQLGDKKLIVQRASVGAKQNLNNPQAMNMVPAQLQIPGLDISMAVPGAVAATEVLALMNMVTPDELGDDEEFEEIYDDVREECSKYGRVKSMEIPRPMEGLMEPPGVGKIFVEFSSIDDAKKAAAALGGRKFANRVVVTSYYPPEEYHRRIF 331
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO33926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQW0]) HSP 1 Score: 76.2554 bits (186), Expect = 7.356e-15 Identity = 61/247 (24.70%), Postives = 100/247 (40.49%), Query Frame = 2
Query: 812 PHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVV------------------------------QRACLGAKNASG------IPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGF 1444
P +L+VG L + E VK + + FG + L+ DS T SKGY F ++ + +A + G +L + + + RA L AK + G +P P +++ T ++ T L NM RD +I +DV EEC+KFG ++ + + D S G ++V+ + A K+L GR F+GK ++ + L Y F
Sbjct: 214 PTRLYVGSLHFNITEAMVKAVFEPFGTVDSVQLIYDSETNRSKGYGFVQFREAEAAKRAMEQMNGFELAGRPLKIGPVTERGDSSAYSFLDDEEYEKGGVELNSSARAALMAKLSQGHSAGLSVPGAPPIVSGVQQALATPVAVSLPTPCFMLTNMFDPTKERDAGWDLDIRDDVLEECNKFGPIVHIHV-------DKNSPQGIVYVKCATPDIAISASKSLHGRWFAGKQIIAAPVPLSNYHTMF 453
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO46306 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQK4]) HSP 1 Score: 73.1738 bits (178), Expect = 8.249e-14 Identity = 43/104 (41.35%), Postives = 62/104 (59.62%), Query Frame = 2
Query: 1124 KISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPR-PTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY 1432
K+S E++V+ L NMV ++DL +D E+E V +ECSKFGTV V I + G +++ + KIFVEF + ET KA L GR F G+ V Y+ +K+
Sbjct: 399 KLSRKSESKVMVLRNMVGVEDLDEDLEHE-----VTDECSKFGTVSRVVIYKEKQGEEEDAEVIVKIFVEFTSPDETDKATSNLDGRWFGGRAVKAEAYNEDKF 497 HSP 2 Score: 55.8398 bits (133), Expect = 2.596e-8 Identity = 49/191 (25.65%), Postives = 84/191 (43.98%), Query Frame = 2
Query: 494 RIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTD------KNFAFLEFRSVEETNQGL-NLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRA 1045
R+YVG++ + EE + F+ PI + ++ D K FAF+E+ E L ++G++ G +K+ RP + +PQA I A ++++ + L ED +K + + FG + +L K+ TG KGY F EY + + A + LG + + V RA
Sbjct: 65 RVYVGSINFELREEHIRTAFH-----------PFGPINKIDLSWDPLNMKHKGFAFVEYDLPEAAQLALEQMNGVLLGGRNIKVGRPSN---VPQAAPLIEQFEQEA----KKYARIYIASVHPDLLEDDIKSVFEAFGKVVHCSLSKEPMTGKHKGYGFIEYENQQSANDAIASMNLFDLGGQFLRVGRA 237
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO25346 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DWR4]) HSP 1 Score: 59.6918 bits (143), Expect = 9.152e-11 Identity = 36/98 (36.73%), Postives = 58/98 (59.18%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPR-PTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYR 1435
++V+ L NMV ++ DD + + EEC+K+G V+ V I P GA D+ + +IFVEF +KA+ L+GR F G+ V +F++L+K+R
Sbjct: 60 SKVVLLRNMVGPGEVDDD-----LQPETAEECAKYGEVVKVIIYEIPVGAPDDEAV--RIFVEFTRMESAIKAVVDLNGRYFGGRTVKANFFNLDKFR 150
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO39985 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8H1]) HSP 1 Score: 59.6918 bits (143), Expect = 9.933e-10 Identity = 36/98 (36.73%), Postives = 58/98 (59.18%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPR-PTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYR 1435
++V+ L NMV ++ DD + + EEC+K+G V+ V I P GA D+ + +IFVEF +KA+ L+GR F G+ V +F++L+K+R
Sbjct: 298 SKVVLLRNMVGPGEVDDD-----LQPETAEECAKYGEVVKVIIYEIPVGAPDDEAV--RIFVEFTRMESAIKAVVDLNGRYFGGRTVKANFFNLDKFR 388
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2 [Source:NCBI gene;Acc:101165173]) HSP 1 Score: 377.096 bits (967), Expect = 8.277e-127 Identity = 196/382 (51.31%), Postives = 267/382 (69.90%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D ++ D+A GL GMQLG+K+++VQRA +G+KNA + I P+ L P + TEVLCL NMV ++L DDEEYEEI+EDV+EEC K+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 59 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGSKNATLTSINQTPVTLQVPGLNSSVTQMGGLPTEVLCLMNMVAPEELLDDEEYEEIVEDVREECGKYGQVKSIEIPRPVDGLE-VPGTGKIFVEFTSVFDAQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 439
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2 [Source:NCBI gene;Acc:101165173]) HSP 1 Score: 377.481 bits (968), Expect = 1.424e-126 Identity = 196/382 (51.31%), Postives = 267/382 (69.90%), Query Frame = 2
Query: 317 YWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD--GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNA--SGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY-RNGFF 1447
YWDV P G+E+++ ++K + + GQ+ + L + +T + P +G+ ++RQARR+YVGN+P G+TEE MM+FFN Q++L LTQA G+P++AVQIN DKNFAFLEFRSV+ET Q + DGI++ G +LK+RRP DY PLP + + + + +V D+ HKLF+GGLP YL +DQVKELL +FGPL+ FNLVKDS TGLSKGYAFCEY D ++ D+A GL GMQLG+K+++VQRA +G+KNA + I P+ L P + TEVLCL NMV ++L DDEEYEEI+EDV+EEC K+G V S++IPRP + V G GKIFVEF + + KAM+ L+GR F+ +VVVT + D + Y R F+
Sbjct: 90 YWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGSKNATLTSINQTPVTLQVPGLNSSVTQMGGLPTEVLCLMNMVAPEELLDDEEYEEIVEDVREECGKYGQVKSIEIPRPVDGLE-VPGTGKIFVEFTSVFDAQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 470
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: uhmk1 (U2AF homology motif kinase 1 [Source:NCBI gene;Acc:101158012]) HSP 1 Score: 96.2857 bits (238), Expect = 4.563e-21 Identity = 46/101 (45.54%), Postives = 69/101 (68.32%), Query Frame = 2
Query: 1145 TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
+ VL L N++ L ++EEYE+IMED+KEEC K+G+V+S+ IP+ G G++FVE+ N S++ +A + L+GRTF GK VV +FY L Y+ G+F
Sbjct: 344 SPVLRLLNLIDDSHLHNEEEYEDIMEDMKEECQKYGSVVSLLIPKEN------PGKGQVFVEYANSSDSKEAQRLLTGRTFDGKFVVATFYPLSAYKRGYF 438
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: uhmk1 (U2AF homology motif kinase 1 [Source:NCBI gene;Acc:101158012]) HSP 1 Score: 95.9005 bits (237), Expect = 5.200e-21 Identity = 46/99 (46.46%), Postives = 68/99 (68.69%), Query Frame = 2
Query: 1151 VLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
VL L N++ L ++EEYE+IMED+KEEC K+G+V+S+ IP+ G G++FVE+ N S++ +A + L+GRTF GK VV +FY L Y+ G+F
Sbjct: 313 VLRLLNLIDDSHLHNEEEYEDIMEDMKEECQKYGSVVSLLIPKEN------PGKGQVFVEYANSSDSKEAQRLLTGRTFDGKFVVATFYPLSAYKRGYF 405
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: rbm39b (RNA-binding protein 39 [Source:NCBI gene;Acc:101167788]) HSP 1 Score: 71.2478 bits (173), Expect = 7.287e-13 Identity = 89/388 (22.94%), Postives = 141/388 (36.34%), Query Frame = 2
Query: 482 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKN------FAFLEFRSVEETNQGLNLDG--------IVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVV-------------------------------QRACLGAKNASG------------------IPTGPLMLTQPMMMDPTKISNVEE----TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGF 1444
R AR ++ L + + +FF+ K+++ V+I +D+N A++EF + L G IV A K R L + G P +L+VG L + E+ ++ + + FG + G L+ DS TG SKGY F +AD KA L G +L + + V R L A+ A G IP G + P++ N+ T L L N+ D EI +DV EEC+K G V+ + + D S G ++V+ + + + AL GR F+ K++ ++ L Y N F
Sbjct: 147 RDARTVFCMQLAARIRARDLEDFFSAVGKVRD-----------VRIISDRNSRRSKGIAYIEFVETSSVPLAIGLTGQRLLGVPIIVQASQAEKNRAAAAANNLQKGSAG--------------PMRLYVGSLHFNITEEMLRGIFEPFGKIEGIQLMMDSETGRSKGYGFISFADAECAKKALEQLNGFELAGRPMKVGHVTERSDSSTASSFLDNDELERTGIDLGTTGRLQLMARLAEGTGLKIPPAAQQALQMTGSIPFGNMAAPAIPTPAPSQALNLPSQPLATHCLQLSNLFDPQAENDPAWASEIQDDVIEECNKHGGVVHIYV-------DKNSPQGNVYVKCPSIPAAMATVNALHGRWFARKMITAAYVPLPTYHNLF 502
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000018652.1 (SMESG000018652.1) HSP 1 Score: 874.774 bits (2259), Expect = 0.000e+0 Identity = 475/476 (99.79%), Postives = 476/476 (100.00%), Query Frame = 2
Query: 20 MSVEYSNQMDKVKEEMSIKREPNYDEPKDKRDRQPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSPNPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
MSVEYSNQMDKVKEEMSIKREPNYDEPKD+RDRQPRRSQEKSESRSRHRSRSHKRKRSNSHEHKSSRRRRSRDRRSRSHERRRRSTSPNPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF
Sbjct: 1 MSVEYSNQMDKVKEEMSIKREPNYDEPKDRRDRQPRRSQEKSESRSRHRSRSHKRKRSNSHEHKSSRRRRSRDRRSRSHERRRRSTSPNPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 476
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000018647.1 (SMESG000018647.1) HSP 1 Score: 874.774 bits (2259), Expect = 0.000e+0 Identity = 475/476 (99.79%), Postives = 476/476 (100.00%), Query Frame = 2
Query: 20 MSVEYSNQMDKVKEEMSIKREPNYDEPKDKRDRQPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTSPNPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
MSVEYSNQMDKVKEEMSIKREPNYDEPKD+RDRQPRRSQEKSESRSRHRSRSHKRKRSNSHEHKSSRRRRSRDRRSRSHERRRRSTSPNPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF
Sbjct: 1 MSVEYSNQMDKVKEEMSIKREPNYDEPKDRRDRQPRRSQEKSESRSRHRSRSHKRKRSNSHEHKSSRRRRSRDRRSRSHERRRRSTSPNPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 476
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000078029.1 (SMESG000078029.1) HSP 1 Score: 604.364 bits (1557), Expect = 0.000e+0 Identity = 288/391 (73.66%), Postives = 337/391 (86.19%), Query Frame = 2
Query: 284 NPLLELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLTEGNVVNMGLTASEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLT--QPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGA-DDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 1447
N +ELKK+YKYWDV PTGYE+MS +EFK + + GQVLP++Y TE +VN ++PSIG+ VSRQARRIYVGN+PLGVTE+GMM+FFNEQ+KLK TQADGDPIIAVQINTDKNFAFLEFRSVEET+QGLNLDGI+Y+GF LKLRRPRDYVPLP D ++ NS+ AG+VQDTPHKLF+GGLP YL EDQVKELL TFGPL GFNLVKDS+TGLSKGYAFCEYADHSVTDKAC+GLTGMQLGEKRIVVQRA LGAKN + T L++ Q + MDP+ + +ETE+LCLHNM+ ++L DDEEYEEI+EDVKEECSK+G+V S+QIPRPTG+ +D+ +GVGKI+VEF NKSETVKAMKAL+GRTFSG+VVVTSFYD E+YRNGFF
Sbjct: 110 NASVELKKDYKYWDVPPTGYEHMSPSEFKAMQAAGQVLPTIYATESGIVNTEQIMDQHPSIGSAVSRQARRIYVGNIPLGVTEQGMMDFFNEQMKLKLFTQADGDPIIAVQINTDKNFAFLEFRSVEETSQGLNLDGIMYNGFPLKLRRPRDYVPLPTTMDTMLPNSLAAGVVQDTPHKLFIGGLPHYLNEDQVKELLLTFGPLSGFNLVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPTTAATTALLIARQQQLGMDPSMGTCGQETEILCLHNMITPEELTDDEEYEEIVEDVKEECSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 500
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000051274.1 (SMESG000051274.1) HSP 1 Score: 395.201 bits (1014), Expect = 6.297e-134 Identity = 219/402 (54.48%), Postives = 276/402 (68.66%), Query Frame = 2
Query: 293 LELKKNYKYWDVAPTGYENMSAAEFKQIHSGGQVLPSLYLT-----EGNVVNMGLTA-SEYPSIGNTVSRQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFD-------GIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMMMDPTKISNVEE---------TEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY 1432
L KK YKYWDV P G++++S A +K + + GQ+ ++Y N N L S+ P G+ V RQARR+YVGN+P G+TE M+E FN Q+K+K L QA+GDP+IAVQIN DKNFAF+EFRSV+ET Q L LDGI + G LKLRRPRDYVPLP D G+VS +VQ+TP+K+F+G LP YL +DQVKELL +FGPLRGFNLVKD TGLSKGYAFCEY D SVTD AC GL GMQLG+K+I+VQRA +GAK+ PMMM P +N E T+VLCL NMV +DL D+EEYE++++D+ EC K+GTV+S++IPRP V GVGKIFVEF + + KA+ AL+GR F+ +VVVTS+YDL+KY
Sbjct: 59 LPSKKAYKYWDVPPQGFDHISPAHYKAMQASGQIPINVYAAGQVPMPDNAPNAPLVLHSDIPFAGSAVCRQARRLYVGNIPFGITETAMIELFNSQMKMKGLMQAEGDPVIAVQINIDKNFAFVEFRSVDETTQALALDGINFQGQQLKLRRPRDYVPLPGLSDNPQMSVPGVVST-----MVQETPNKVFIGSLPNYLTDDQVKELLLSFGPLRGFNLVKDGATGLSKGYAFCEYVDQSVTDIACAGLNGMQLGDKKIIVQRASIGAKHG----------LIPMMM-PFVNANFESLIAAGGVGATKVLCLMNMVDEEDLVDEEEYEDVVDDINSECGKYGTVVSLEIPRPN-PTMLVGGVGKIFVEFASVIDCQKAVAALTGRKFNNRVVVTSYYDLDKY 443
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000003347.1 (SMESG000003347.1) HSP 1 Score: 82.0333 bits (201), Expect = 1.660e-16 Identity = 89/338 (26.33%), Postives = 145/338 (42.90%), Query Frame = 2
Query: 482 RQARRIYVGNLPLGVTEEGMMEFFNEQIKLKELTQADGDPIIAVQINTDKNFAFLEFRSVEETNQGLNLDGIVYHGFALKLRRPRDYVPLPQAFDGIVSNSMTAGIVQDTPHKLFVGGLPQYLGEDQVKELLQTFGPLRGFNLVKDSTTGLSKGYAFC-----EYADHSVTDKACMGLTGM--------------QLGEKRIVVQRAC--LGAKNASGIPTGPLMLTQPMMMDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGADDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKY 1432
+ AR IY+ L L V + +FF + K+ + ++ + + AF++FR VE N+ L+L+G G +++R +A +G +++S I P KL V L + E ++E+ +G + L+KD G S G AF E A + V L G + GE + V A LG + TG L + T I N ++ L NM + D +D+ +E+ +DV EC++FG V V I R S G+I+V+ + K + L GR+F G+V+ ++ LE Y
Sbjct: 122 KDARTIYIWQLSLRVRTRDLEDFFVQVGKMDVVR-----LVLDNKTGKSRGVAFIQFRDVESVNKALDLNGQKLMGVPIQIRTVSS--EKCRAAEGTMNSSY---IYFRGPTKLCVSNLHPQINEQMIEEIFAPYGKIDELRLMKD-IRGTSLGTAFILFRKGENAKNVVEQMNDFNLAGQLLKIAPVVEEGGLNEFGENQGVSMTAADRLGLMAKLAVGTG-LQVVPKANAVQTPIIN---SQCFVLSNMFDVKDAHEDKFCKEVQDDVVGECNQFGGVYHVHIDRK-------SPRGEIYVKCPSIYVANKCVSTLHGRSFDGRVITANYIPLEHY 437 The following BLAST results are available for this feature:
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007734 ID=SMED30007734|Name=U2 snRNP auxiliary factor large subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=1487bpback to top protein sequence of SMED30007734-orf-1 >SMED30007734-orf-1 ID=SMED30007734-orf-1|Name=SMED30007734-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=477bp MSVEYSNQMDKVKEEMSIKREPNYDEPKDKRDRQPRRSQEKSESRSRHRSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
