G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30007707
Length (bp)1747
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30007707) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30007707) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30007707) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30007707) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007707

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30007707h1SMcG0001526 dd_Smed_v4_17965_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007707h1SMcG0001526 dd_Smed_v4_17965_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30007707h1SMcG0001526 dd_Smed_v4_17965_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007707h1SMcG0001526 dd_Smed_v4_57112_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30007707h1SMcG0001526 dd_Smed_v4_57112_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30007707h1SMcG0001526 dd_Smed_v6_17965_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: R7U5B9 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_201825 PE=4 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 2.842e-44
Identity = 121/379 (31.93%), Postives = 194/379 (51.19%), Query Frame = 1
Query:  304 NSSLNILVRYNHQSMNIYHNFTYIYTM-EYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISM---KNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIK----NRSSSTMTRNSCGNVHIANISAVFTPLKK 1416
            N SL++  RY H   N+Y  FT + T  +   +P+W  +GIPG+I+ F IW   +   SS  YL  L+++DL+F    F F L N   +   + P +C+ FP I +  QY  P+L LGF+VER +S+CHPF  ++  + +  V+    L+  CVLL   + YF+ Y  E ++   C+V    I L +        +I   +  +TE +VF +VPL I ++NI VI E+  M   + +R+       +    +A+T  LL+VSFYL+  T+P TV   L  N       ISD ++  +  W+ H    +++  +E    ++ A  F++Y++T + FR  F+ +F  K      +SSTM            +SA   PL+ 
Sbjct:   87 NESLDL--RYRHP--NLYEEFTPLATFADKYLLPVWYVVGIPGNILAFIIWIQPKMRPSSGCYLAALAMDDLVFLFLKFFFELTNTWALPIFNVPVLCEVFPVIFLASQYYDPVLVLGFTVERYISICHPFQREKFCSTRRAVKVIAGLLLCCVLLHAVQGYFWYYSSETQQ---CSVRLA-ITLGNNA------SIWTVWSWITELLVFGLVPLTILILNIMVILEVRKMSASEEKRMCLKKGHKTGGQTSATTFMLLVVSFYLIFTTLPVTVCYALNLNFREGEFLISDEELLTDSTWQRHFNFTTVRRLLENFGMSHYACNFYLYMMTGKVFRKEFRRIFLAKCTKDKSASSTM-----------GLSAASAPLRD 440          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0K0PUI8 (Orphan G-protein coupled receptor 50 OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 4.586e-36
Identity = 97/341 (28.45%), Postives = 180/341 (52.79%), Query Frame = 1
Query:  301 SNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPK-NLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMF 1320
            +  + ++L RY  + +   +  T     +YI  P+W  +G P +++ F +W   R   SS  YL  L+++D++F I H +F L     ++ +  P +C+ FP   +  QY +PLL L F+VER +++CHPF  ++       ++    L+ + + +   + YF+ Y  + +E   CT+       +D+ ++   +++   +  +TE +VF +VP++I ++NI VI E   +          +  +  +AA T TLL VSFYL+  T+P T+F  + + +FP+ +  +SD +I  +  W+ H  +  I+  VE L  ++ A  FFIY+ T + FR    ++F
Sbjct:   20 TEGTQHVLQRYTQEQLYAIYRPTISIITKYI-TPVWYVIGFPSNMLAFTVWVQPRMRPSSGCYLAALAMSDVIFLILHLVFELQMTWDVRLLKLPVLCEVFPIFFLSSQYMSPLLVLAFTVERFIAICHPFRREKFCTTSRAIKVIIGLVILSMCIHAVQGYFWIYRADIDE---CTI-------RDEMMLGGTKSVWSVWSWITELLVFGLVPVVILILNILVIKEAQKLSRTEESRLCLRRGHKTSAA-TLTLLSVSFYLILTTLPVTLFYAM-YLSFPEGDFFMSDDEIAADPTWQRHFTYFLIRTLVEELGMSHFACNFFIYVSTGKLFRRELTVLF 347          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A1S3HS84 (probable G-protein coupled receptor 139 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106157323 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 2.324e-33
Identity = 107/385 (27.79%), Postives = 188/385 (48.83%), Query Frame = 1
Query:  289 NGNCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYI---KTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSN----FQMMFSIKNRSSSTMTRNSCGNVHIANISAVFTPLKKSK 1422
            NG+ S S ++I     H  + I        T+E     ++   G+ G+ + F +W  RR  HSS  YL  L+++DL F + H L+     HYI   K  +    C+ F  + +  Q+ +P+  L F++ER ++VCHPF  D+I      ++    +  +C+L+   + YF++Y  + ++   C       +++ + LI  + ++   +  VTE ++FAVVPL++ + NI VI E   +     I   S+      + +T  LL VSF+L+  T+P T+   L       +  +SD +I L+ +W+ HL++ +I+  V+     + A  F+IY +T + FR      F+ +F I+   S   T NS    H    S     L+ S+
Sbjct:   56 NGSVSRSEIDIY--QVHLPLKI--------TLEQWVPIVYYIFGLSGNFLAFIVWIQRRMQHSSGCYLAALALSDLCFLLLHMLY---EIHYIWGHKVFNANGACQIFAIVFMWTQHMSPMFVLAFTIERYLAVCHPFKRDKICTKSLAIKVIVSITVVCLLIDGMQGYFWTYVSKADD---C-------DVRGETLIGGVGSVWSVWSWVTEILIFAVVPLLVLIFNILVIHEARKLSENEKIQLQSRKG-RRTSTTTFMLLAVSFFLIFTTLPVTIVYNLYAVFQQGDPTLSDEEIHLDQKWQAHLKYTAIKTLVQYFGMAHYACNFYIYCITGKMFRKELVLIFKRVFRIQIPVSRRGTFNSSLRTHSTGFSLRSGALRASE 416          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267H1H3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030285g1 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 8.892e-33
Identity = 111/339 (32.74%), Postives = 178/339 (52.51%), Query Frame = 1
Query:  328 RYNHQSMNIYHNF-TYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHY-IKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVF---QLLKFNNFPKNLC-ISDSDIW---LNYEW---KMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNF 1308
            RYN  ++ +   F   + T   I +P+W+ LG+PGS++   +WS +    SS +YLC L+I DL+F +   L +    +Y IK + NPFMC+ FP  N+ LQY +PLLTLGF+VER + +CHPF        +  +     +   C++L L +L+FY Y   N  + +C+   + + + D +  +        +  +TE + FA+ P+ I + NI V+ E   +  +R   T  + S    AA+T TLL VSFY + A +P T+     L      P+  C I DSD      + +W   + +L ++++   V+ LA ++ A KF IY+ T Q FR   
Sbjct:   19 RYN-STLGVCSAFLPGVVTFARIIIPMWIALGVPGSLLSLLVWSSKPMRSSSSVYLCALAILDLMFLLCQQLPFHLYLYYDIKVMYNPFMCRAFPVYNMWLQYLSPLLTLGFTVERYIGICHPFSRKEFCTVRRAIIVVSCMTVACLVLGLVQLHFYRY---NPVEATCS---EDLRIFDPSTFE--HRFYSGWSKLTEVLFFALAPVAILVFNILVLVEARRVIRKRPAGTGQRKS----AATTATLLTVSFYQIGAVLPVTILLFVGLAILTEAPQLPCGIPDSDALHQITDGQWPVARSYLTYLTVSRIVDCLAVSHYAMKFVIYLATGQGFRRQL 344          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A1S3K6M5 (probable G-protein coupled receptor 139 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106179100 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.718e-32
Identity = 98/348 (28.16%), Postives = 174/348 (50.00%), Query Frame = 1
Query:  289 NGNCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYI---KTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFS 1323
            NG+ S S ++I     H  + I        T+E     ++   G+ G+ + F +W  RR  HSS  YL  L+++DL F + H L+     HYI   K  +    C+ F  + +  Q+ +P+  L F++ER ++VCHPF  D+I      ++    +  +C+L+   + YF++Y  + ++   C V       + + LI  + ++   +  VTE ++FAVVPL++ + NI VI E   +     I   S+      + +T  LL VSF+L+  T+P T+   L       +  +SD +I L+ +W+ HL++ +I+  V+     + A  F+IY +T + FR    ++F 
Sbjct:   56 NGSVSRSEIDIY--QVHLPLKI--------TLEQWVPIVYYIFGLSGNFLAFIVWIQRRMQHSSGCYLAALALSDLCFLLLHMLY---EIHYIWGHKVFNANGACQIFAIVFMWTQHMSPMFVLAFTIERYLAVCHPFKRDKICTKSLAIKVIVSITVVCLLIDGMQGYFWTYVSKADD---CDV-------RGETLIGGVGSVWSVWSWVTEILIFAVVPLLVLIFNILVIHEARKLSENEKIQLQSRKG-RRTSTTTFMLLAVSFFLIFTTLPVTIVYNLYAVFQQGDPTLSDEEIHLDQKWQAHLKYTAIKTLVQYFGMAHYACNFYIYCITGKMFRKELVLIFK 379          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000032057.1 (SMESG000032057.1)

HSP 1 Score: 783.097 bits (2021), Expect = 0.000e+0
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 1
Query:  280 MVENGNCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIKNRSSSTMTRNSCGNVHIANISAVFTPLKKSKLFYSNEMEKVYNKCPK 1470
            MVENGNCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDLLFFIFHFLFYLNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIKNRSSSTMTRNSCGNVHIANISAVFTPLKKSKLFYSNEMEKVYNKCPK
Sbjct:    1 MVENGNCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDLLFFIFHFLFYLNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIKNRSSSTMTRNSCGNVHIANISAVFTPLKKSKLFYSNEMEKVYNKCPK 397          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000012527.1 (SMESG000012527.1)

HSP 1 Score: 207.223 bits (526), Expect = 6.671e-61
Identity = 138/362 (38.12%), Postives = 216/362 (59.67%), Query Frame = 1
Query:  295 NCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRIS--NYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIKNRS-----SSTMTRN 1359
             C N SL++  R+    + +Y++FT  +  E I +PI +CLG+PG+I  F IW+ ++  +SS +YL  L+I+D LF I H +++L++ + I  I+N F C  FP  N++LQY +P+LTL F+VER +S+C PF  +R+   N   TV +   + S C+  ALP LYF+ ++  N  +   T+Y  Y   K  N   +  + +  Y I+TE+++FA +P +I ++NIRVI E+  +         S    S+  A+T TLL VSFYL+CA++P T+  L  FN    NLC SDS+I  +  W+ H R+V++Q+ +E  + TY  AKF+IY+ TS+ FR+ F  +F   N++     SS M R 
Sbjct:    8 KCINGSLDVYGRHK-SDLGLYNHFTVKHLFEQITVPILVCLGLPGTIFSFIIWTRKQMRYSSSIYLISLAISDSLFIIGHIIWHLDSTYNIPVINNRFTCNIFPVFNLLLQYMSPILTLAFTVERYISICWPFSKERLCSINRAITVSSVLFIKSFCI--ALPMLYFFRFDSTNINR-CLTIYEIY---KSSNPKDESTDWLFIYNIITESIMFACIPFIILIINIRVIIEMWKIYKSSKTGIRSSER-SHKGATTVTLLSVSFYLICASLPLTIVHLCGFNLEEGNLCASDSEILADAIWRKHFRYVNVQVFIEFFSLTYYGAKFYIYLGTSEAFRTAFLQLFVPDNKAFPDSKSSRMDRK 361          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000066742.1 (SMESG000066742.1)

HSP 1 Score: 174.096 bits (440), Expect = 1.140e-48
Identity = 118/374 (31.55%), Postives = 203/374 (54.28%), Query Frame = 1
Query:  289 NGNCSNSSLNI--LVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVI-------------FELISMKNRRLISTASQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNF-PKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIKNRSSSTMTRNS 1362
            + +C N S+N    V      M +Y  FT   ++  I +PIWL LGIPGSI C  +W+  +  H+S +YL  L  +DL FFI  F+++L     I  ++ P +C+ F  IN  LQY +PL T  F++ER + +C PF  +R  +    +    ++  I  LL+L + YF+ Y+ E +E    +   Q  E   QN       ++  + ++TET++F ++PL I ++N+ VI             F+LI  +NR+ +   S+ + + NA++T  LL +SFY + +T+P T+ QLL  +NF   + C++ ++++ +  W+ H + V+++  +E LA ++ +AKF+IY++T   FR  F  +F   +       RN 
Sbjct:    4 SDDCQNKSVNFTKFVNLFGDFMGVYEAFTIKGSLHRIIIPIWLILGIPGSIFCVIVWTRPKMLHTSSVYLAALGASDLGFFICQFVWFLKTAWRIDLLNVPVLCELFAVINFFLQYLSPLFTFAFTLERFILICFPFKGERFLSVPRAITVTLVITLIAFLLSLIQGYFFEYKEEIKECIERSSVVQ--EGTWQN------RLLFYWNLITETVIFGLIPLSILILNLFVIKETKILQSNAPVKFKLI--ENRQNV-INSKTTDNRNASTTVMLLSISFYFILSTLPVTIMQLLIIHNFWNGDECLTVTEVYSDPIWQAHFKAVNVKSVIETLALSHYSAKFYIYMITGVQFRIQFLNIFKRHSNKQILFRRNG 366          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042511.1 (SMESG000042511.1)

HSP 1 Score: 171.014 bits (432), Expect = 1.062e-47
Identity = 114/355 (32.11%), Postives = 188/355 (52.96%), Query Frame = 1
Query:  298 CSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPGSIICFRIWSIRRQHHS-SVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYI----ELK------DQNLIKKLR-NIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTA--SQPSYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMF 1320
            C N S +  +R+    +   + FT  Y +E +F+P W  LG PG++I   IW+    HH  S LYL +L++ D LFF  H +++L + + I TI N  MC  FP IN++LQY +PLL LGF+VER +SVC+P    ++                C +     +YFY Y+   +    C     ++    EL+       Q L    R +    + +++E+ +F V+P +I  +NI VI +L  +   R I T   S+       ++T +LL VSF+L+  ++P ++ Q++K +    + C+SD +I  +  WK +  + +IQ+ +EML+ T+ AA F+IY+ T + FR  F  +F
Sbjct:    7 CLNGSKSAYMRHK-SDLEFDNQFTIKYRLEVVFIPTWFVLGFPGNLISIYIWTRYSMHHMVSSLYLAVLAVVDCLFFGRHLIWHLQSIYNIHTIKNHVMCHLFPMINMVLQYLSPLLILGFTVERFISVCYPLYRRKL----------------CTVERTKHIYFYHYKNFLDLTDDCVDIVSWLMSQYELQFNSSSAKQQLQTDWRYHFFNMFNLISESFIFIVIPFLILFLNIAVIVKLNQVHKFRRILTRNFSKKHEDRQLSATISLLSVSFFLIITSLPTSIVQMMKLDLTKDDTCLSDDEIRRDPAWKAYFLNKNIQVQIEMLSLTHYAANFYIYLCTCRQFREQFIRLF 344          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000018637.1 (SMESG000018637.1)

HSP 1 Score: 98.9821 bits (245), Expect = 4.513e-22
Identity = 85/332 (25.60%), Postives = 156/332 (46.99%), Query Frame = 1
Query:  376 YTMEYIFMPIWLCLGIPGSIICFRIWSIR--RQHHSSVLYLCLLSINDXXXXXXXXXXXXNNYHYIKTIDNPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTVRTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKKLRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISM--------------KNRRLISTASQPSYSNN------AASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWLNYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSN 1305
            Y ++   +PI+L +GI G+ I F +W  R  R+++SS +Y+ +L+IND    I   ++YL +      ID   +CKF+  I   LQY + LL  GF++ER ++VC+PF  +R          C ++ S   +   P  + + Y+        C+V   +IE              +TY +  E +  A++P++   VNIRV+ E+  +              +N  + S  S+    N        ++T TLL+VSF+ +  T+P     + +   +P+    S   +  +  WK H +    +  +++L+ ++      IY+ +S  FR++
Sbjct:   25 YQLQLYSIPIFLTVGILGNPISFVVWVSRKLRRNNSSAVYIAMLAINDFFVVICSIIYYLRHSWGFGIIDIRGICKFYMVIFTFLQYNSILLVFGFTIERWIAVCYPFRRERCCTENRAWIACGIIFSSVFISVFPLFFVWHYQ------PLCSVPKHWIE----------NTYFVTYSLTLEILFSALLPVITLAVNIRVVVEMYRISETIVTFPKLSAVNQNFEIPSQKSRKHSKNTENSISFKSTTITLLLVSFFQILTTLPLGAVYICQI-FYPEGNATSVKQMQNDPIWKKHFQMHGAKDIIDLLSLSHYCFNIVIYLASSHLFRNH 339          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7U5B92.842e-4431.93G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0K0PUI84.586e-3628.45Orphan G-protein coupled receptor 50 OS=Platynerei... [more]
A0A1S3HS842.324e-3327.79probable G-protein coupled receptor 139 isoform X1... [more]
A0A267H1H38.892e-3332.74G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A1S3K6M52.718e-3228.16probable G-protein coupled receptor 139 isoform X2... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032057.10.000e+0100.00SMESG000032057.1[more]
SMESG000012527.16.671e-6138.12SMESG000012527.1[more]
SMESG000066742.11.140e-4831.55SMESG000066742.1[more]
SMESG000042511.11.062e-4732.11SMESG000042511.1[more]
SMESG000018637.14.513e-2225.60SMESG000018637.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007707 ID=SMED30007707|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1747bp
GGTTAATTGCCTCACACCTTAATAGATTCCTCCAATTGCATTTGTTTAAT
TGGATTGGTTCCCTCTTCCTTATTTATAGGAAATTTTCATTTTTTTTCTC
ATTTTACTTCAGTTTATTTTCCAATAAAATATTCGATAAATTTTGGTACA
AAAAATATTTCACTATTTCTGGATATAAAGAAGTCATTTTGATAGACAAC
TGTCAAGGACAATATTGTGATTATCTTCAACTATTAATTGGAAAATTTGT
TCAGAAAAATTTGTTGATTTTGTAAAACAATGGTCGAAAACGGAAATTGT
TCCAACTCGTCATTGAACATTTTAGTACGATATAACCACCAGTCGATGAA
TATTTATCACAACTTCACTTACATTTACACAATGGAATACATATTCATGC
CAATCTGGCTCTGCCTCGGAATTCCTGGTTCAATTATATGCTTCCGCATA
TGGTCTATTCGTCGTCAACATCATTCTTCTGTGTTATATTTATGCCTTCT
ATCGATAAATGATTTGCTATTTTTTATCTTTCATTTTTTGTTTTATCTCA
ACAATTATCACTACATTAAAACAATAGACAATCCTTTTATGTGTAAATTT
TTTCCATTTATCAACATAATATTACAGTACGCTGCCCCATTATTGACGTT
GGGATTTTCAGTTGAACGATGCGTTTCCGTTTGTCATCCGTTCATTAGTG
ACAGAATTTCTAATTATAAAACGACGGTTCGGACTTGTTTTTTGTTGATT
TCGATCTGTGTTTTATTGGCTTTACCAAAATTGTATTTTTACTCATATGA
AGGCGAGAATGAAGAAAAAGGAAGTTGTACAGTGTACTATCAGTATATTG
AATTAAAGGATCAAAATTTAATCAAAAAACTAAGAAATATTATGTTGACT
TATGAAATAGTTACAGAAACAATGGTCTTCGCCGTGGTTCCTTTAATGAT
CTTCCTAGTAAATATCCGTGTCATATTTGAGTTGATTTCAATGAAAAATC
GTAGACTGATCTCTACGGCTTCCCAACCATCGTATAGTAACAACGCGGCA
AGTACAACTACTCTGCTAATAGTCAGTTTTTATTTGTTGTGCGCCACAAT
TCCCGCAACAGTATTTCAACTGCTGAAATTTAACAATTTCCCCAAAAACC
TCTGCATTTCTGATTCGGATATTTGGTTGAATTATGAATGGAAAATGCAT
TTGCGTCATGTGTCAATACAAATCCCTGTGGAGATGCTGGCCAAAACATA
TAATGCCGCTAAATTTTTCATCTATATTGTGACGAGTCAATGGTTTCGAA
GTAATTTTCAGATGATGTTTTCAATAAAGAACCGTTCATCGTCGACAATG
ACGAGGAACAGCTGCGGAAATGTCCATATTGCGAATATTTCAGCCGTTTT
CACACCTTTGAAAAAATCCAAGCTTTTCTACTCTAATGAAATGGAAAAGG
TTTACAATAAATGTCCAAAATGAAAACATTTCTTTTAAGTTTTTTTTGTT
ATTGTTATCATTGTTTTTGATTTTTTTGCAAATGTAAAGTTTTGAGAAAA
TTTTGGAAAAAAATATCTGAAAATTTTTCATTGTGTTTTGTATTTTATTC
AAACGCTTTATGTGACGCATATTATGAAATAACTTTACGTATTGTTTTAT
TAATATTTTCTTGGCCCATCTTAATAAATAATTTTCAGGAATTATATTTA
TATTTGATGACACTTCCTGTGGGAAAAACACATTTGTCGCGCAAATT
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protein sequence of SMED30007707-orf-1

>SMED30007707-orf-1 ID=SMED30007707-orf-1|Name=SMED30007707-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp
ICATNVFFPQEVSSNINIIPENYLLRWAKKILIKQYVKLFHNMRHIKRLN
KIQNTMKNFQIFFSKIFSKLYICKKIKNNDNNNKKNLKEMFSFWTFIVNL
FHFIRVEKLGFFQRCENG*
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protein sequence of SMED30007707-orf-2

>SMED30007707-orf-2 ID=SMED30007707-orf-2|Name=SMED30007707-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=398bp
MVENGNCSNSSLNILVRYNHQSMNIYHNFTYIYTMEYIFMPIWLCLGIPG
SIICFRIWSIRRQHHSSVLYLCLLSINDLLFFIFHFLFYLNNYHYIKTID
NPFMCKFFPFINIILQYAAPLLTLGFSVERCVSVCHPFISDRISNYKTTV
RTCFLLISICVLLALPKLYFYSYEGENEEKGSCTVYYQYIELKDQNLIKK
LRNIMLTYEIVTETMVFAVVPLMIFLVNIRVIFELISMKNRRLISTASQP
SYSNNAASTTTLLIVSFYLLCATIPATVFQLLKFNNFPKNLCISDSDIWL
NYEWKMHLRHVSIQIPVEMLAKTYNAAKFFIYIVTSQWFRSNFQMMFSIK
NRSSSTMTRNSCGNVHIANISAVFTPLKKSKLFYSNEMEKVYNKCPK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000421tail
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 54..285
e-value: 4.2E-15
score: 55.8
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 17..357
e-value: 2.2E-30
score: 107.7
NoneNo IPR availablePANTHERPTHR24243G-PROTEIN COUPLED RECEPTORcoord: 20..357
NoneNo IPR availablePANTHERPTHR24243:SF208ETHR, ISOFORM Acoord: 20..357
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 38..343
e-value: 4.38154E-17
score: 78.8288
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 24..361
NoneNo IPR availableTMHMMTMhelixcoord: 210..232
NoneNo IPR availableTMHMMTMhelixcoord: 109..128
NoneNo IPR availableTMHMMTMhelixcoord: 262..281
NoneNo IPR availableTMHMMTMhelixcoord: 67..89
NoneNo IPR availableTMHMMTMhelixcoord: 38..60
NoneNo IPR availableTMHMMTMhelixcoord: 149..171
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 50..332
score: 17.423