Degenerin unc-8

Overview
NameDegenerin unc-8
Smed IDSMED30007660
Length (bp)1681
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Degenerin unc-8 (SMED30007660) t-SNE clustered cells

Violin plots show distribution of expression levels for Degenerin unc-8 (SMED30007660) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Degenerin unc-8 (SMED30007660) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Degenerin unc-8 (SMED30007660) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007660

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30007660SMESG000073267.1 dd_Smed_v4_22386_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007660SMESG000073267.1 dd_Smed_v4_22386_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007660SMESG000073267.1 dd_Smed_v4_22386_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30007660SMESG000073267.1 dd_Smed_v4_22386_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30007660SMESG000073267.1 dd_Smed_v4_22386_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007660SMESG000073267.1 dd_Smed_v4_31763_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007660SMESG000073267.1 dd_Smed_v4_31763_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30007660SMESG000073267.1 dd_Smed_v4_31763_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30007660SMESG000073267.1 dd_Smed_v4_31763_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007660SMESG000035755.1 Contig5289GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007660SMESG000073267.1 Contig5289GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neuronSMED30007660SMESG000073267.1 dd_22386dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 4.147e-7
Identity = 40/183 (21.86%), Postives = 77/183 (42.08%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAII 1500
            C +  +QK+ VE C C  P  P+ +      + C       R  + +    +  +Y++ K   +       +C  Y  + C E  Y++  S    P         E    +F+C       L      + MV++  +  +   + + E   Y  +++L+D+GGL GLW+G S + ++E + +I
Sbjct:  515 CHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVNMDQRECLRNTTLWLGELYSKGKEAII------PDC--YCHQPCQETNYEVTYSSARWPSG--SAKVMECLPGDFLC-------LEKYRKNAAMVQIFYEELNY--ETMQESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 678
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 4.147e-7
Identity = 40/183 (21.86%), Postives = 77/183 (42.08%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAII 1500
            C +  +QK+ VE C C  P  P+ +      + C       R  + +    +  +Y++ K   +       +C  Y  + C E  Y++  S    P         E    +F+C       L      + MV++  +  +   + + E   Y  +++L+D+GGL GLW+G S + ++E + +I
Sbjct:  515 CHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVNMDQRECLRNTTLWLGELYSKGKEAII------PDC--YCHQPCQETNYEVTYSSARWPSG--SAKVMECLPGDFLC-------LEKYRKNAAMVQIFYEELNY--ETMQESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 678
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.5286 bits (127), Expect = 4.166e-7
Identity = 40/183 (21.86%), Postives = 77/183 (42.08%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAII 1500
            C +  +QK+ VE C C  P  P+ +      + C       R  + +    +  +Y++ K   +       +C  Y  + C E  Y++  S    P         E    +F+C       L      + MV++  +  +   + + E   Y  +++L+D+GGL GLW+G S + ++E + +I
Sbjct:  553 CHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVNMDQRECLRNTTLWLGELYSKGKEAII------PDC--YCHQPCQETNYEVTYSSARWPSG--SAKVMECLPGDFLC-------LEKYRKNAAMVQIFYEELNY--ETMQESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 716
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.5286 bits (127), Expect = 4.166e-7
Identity = 40/183 (21.86%), Postives = 77/183 (42.08%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAII 1500
            C +  +QK+ VE C C  P  P+ +      + C       R  + +    +  +Y++ K   +       +C  Y  + C E  Y++  S    P         E    +F+C       L      + MV++  +  +   + + E   Y  +++L+D+GGL GLW+G S + ++E + +I
Sbjct:  553 CHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVNMDQRECLRNTTLWLGELYSKGKEAII------PDC--YCHQPCQETNYEVTYSSARWPSG--SAKVMECLPGDFLC-------LEKYRKNAAMVQIFYEELNY--ETMQESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 716
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Celegans
Match: unc-105 (Degenerin-like protein unc-105 [Source:UniProtKB/Swiss-Prot;Acc:Q09274])

HSP 1 Score: 53.1434 bits (126), Expect = 4.452e-7
Identity = 40/183 (21.86%), Postives = 77/183 (42.08%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAII 1500
            C +  +QK+ VE C C  P  P+ +      + C       R  + +    +  +Y++ K   +       +C  Y  + C E  Y++  S    P         E    +F+C       L      + MV++  +  +   + + E   Y  +++L+D+GGL GLW+G S + ++E + +I
Sbjct:  601 CHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVNMDQRECLRNTTLWLGELYSKGKEAII------PDC--YCHQPCQETNYEVTYSSARWPSG--SAKVMECLPGDFLC-------LEKYRKNAAMVQIFYEELNY--ETMQESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 764
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Xenopus
Match: ASIC1 (acid sensing ion channel subunit 1 [Source:NCBI gene;Acc:100489966])

HSP 1 Score: 50.0618 bits (118), Expect = 8.903e-6
Identity = 114/541 (21.07%), Postives = 193/541 (35.67%), Query Frame = 1 
Query:    4 ELNFSSKLLMNKLIENCTIHGLNHIYL-EKSKPAKFSWIVLFVFGICGITLNIV------------LISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLIL---SSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQI-TNNIPCYTYRIEN-----LKLTDDRDISSITMRVRFDYDKYQLF----DEQYVYGGVFLYITNKNELP--QKKEIILQPGNDYEITLEQFRIEKLKKR-NPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYK-----IRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVNKL 1524
            EL     L +       T+HGL HI+  E+    +  W++ F+ G   + L +             +  L  +S P +I          +P +TFCN      LN    S     ++      L+  + NN Y E+   Q+A   ++E    IL   ++  N K K    R F++   + I               +L      G   S   FV + T    CYT+         L  T     + + + +    D+Y       DE     G+ + I +++E P   +    + PG    ++ ++ R+  L      C++     E +D   +S        IDCE     +  VE CNC    +P                        D A C   +Y +     L  +  K          CN  +Y      ++I  + S ++    +   NK E ++ EN    D+  +A   + +               +++ Y  + LL D+GG MGL++G S + V+E     Y  L  KL
Sbjct:    8 ELGSPQPLSVQAFASGSTLHGLAHIFTYERFSCRRLIWVLCFL-GSLSLLLYVCTERIIYYFEYPHVTKLDEISTPRMI----------FPAVTFCN------LNEFRFSRVTKNDLYHAGELLS--LLNNRY-ELPDSQLADEKVME----ILQDKANFRNFKPKPFNMREFYDRTGHDIRE-------------MLLSCKYRGEECSVDDFVPVFTRYGKCYTFNSGRNGRSPLVTTKGGTGNGLELMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRLTYLPPPWGDCKSSAMDSEFFDTYSISAC-----RIDCET----RYLVENCNCRMVHMPG-----------------------DAAYCTPELYKECADPALDFLVEKDNDFCVCETPCNMTRYGKELSMVKIPSKASARYL---AKKYNKSEQYIAENILVLDIFFEALNYETIE--------------QKKAYEVAGLLGDIGGQMGLFIGASILTVLELFDYAYEVLRYKL 462
[Close]
BLAST of Degenerin unc-8 vs. UniProt/SwissProt
Match: sp|Q09274|UN105_CAEEL (Degenerin-like protein unc-105 OS=Caenorhabditis elegans OX=6239 GN=unc-105 PE=1 SV=3)

HSP 1 Score: 53.5286 bits (127), Expect = 5.300e-6
Identity = 40/183 (21.86%), Postives = 77/183 (42.08%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAII 1500
            C +  +QK+ VE C C  P  P+ +      + C       R  + +    +  +Y++ K   +       +C  Y  + C E  Y++  S    P         E    +F+C       L      + MV++  +  +   + + E   Y  +++L+D+GGL GLW+G S + ++E + +I
Sbjct:  553 CHRSCAQKVIVEACGCADPMYPVAEMFGNNTKPCQAVNMDQRECLRNTTLWLGELYSKGKEAII------PDC--YCHQPCQETNYEVTYSSARWPSG--SAKVMECLPGDFLC-------LEKYRKNAAMVQIFYEELNY--ETMQESPAYTLTSVLADLGGLTGLWIGASVVSLLEIVTLI 716
[Close]
BLAST of Degenerin unc-8 vs. UniProt/SwissProt
Match: sp|O01635|ASIC1_CAEEL (Degenerin-like protein asic-1 OS=Caenorhabditis elegans OX=6239 GN=asic-1 PE=3 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 7.369e-6
Identity = 49/202 (24.26%), Postives = 77/202 (38.12%), Query Frame = 1 
Query:  952 CEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRF---LVNKLRIPRNPSQ 1548
            C++   QK   + C C  P  P Y+     P              +D+      I N+         R  K+      + CN++ Y +  S    P      S          C N        +  GS M+ +  ++ +     LLE E Y +SNLLSD GG +GLW+G+S I + E     +     L++  R  R P++
Sbjct:  625 CQRSCIQKHLSKTCGCGDPRFPPYRESKNCP--------------VDDPYKRECIKNEMH----VATRDSKKLGCSCKQPCNQDVYSVSYSASRWPAIAGDLSGCPLGMAAHHCLN------YKREQGS-MIEVYFEQLNYES--LLESEAYGWSNLLSDFGGQLGLWMGVSVITIGEVACFFFEVFISLISSNRTKRRPAR 799
[Close]
BLAST of Degenerin unc-8 vs. TrEMBL
Match: S5W2R2 (Asic OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 2.667e-100
Identity = 188/500 (37.60%), Postives = 290/500 (58.00%), Query Frame = 1 
Query:   31 MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAP--IDLNGEPLSLSYWLNMSQEINELAREISNNNYL-EVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENK------YENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIY 1503
            M  LI++ TIHG+ HI+   +K  K  W +LF+ G+  I +NIV IS+++LSNP++I+++ +   F WPD+TFCNP+ P    +N +P    +W+    +   +A +++    + +++  +      LE RT+ +SS D AKL+    + +   I Y  +H G+YF++ ++  +       +   L ++Q VQ+ NNIPC+T+ ++  ++   + I S+  R + + + Y++F+  Y +  +FLYI+NK+E+PQ  EIIL PG +  + L Q R      R  CR EKF  ELYD  L S   Y G Y DC+K VSQ+ +V+ CNCY+ FLPI+KN SGFPR+C NA+ +S +E+L NAKCM N + ++  +D    R    C  Y    CN+  YKI   KE     +   +N   +      Y      N TS D + K    ++++L+I R ++  DL  EE EYPFS LLSDVGGLMGLWLG+S IG+ E +  +Y
Sbjct:    1 MIDLIQSSTIHGIGHIFSATNKIFKVLWCILFLVGVIAIIVNIVFISMKFLSNPVIISYTFEHEPFIWPDITFCNPTDPYAFTINSQPYL--HWIETLNKTKRVAGKVARQTSMSQLIHSRRENDYELEERTIAMSSADPAKLQATAFQNYLYRIGYIKAHKGLYFSKEFQSSSTKLNE--DWKSLFTSQIVQMRNNIPCHTFHLDKSRIVKQKFIKSLLFRFKLNEESYKIFNSSYTFQSMFLYISNKDEIPQSNEIILTPGYNNVVQLTQNRHSYTGLRRTCRNEKFEAELYDSLLQSTEKYSGSYDDCKKIVSQQKYVKYCNCYNSFLPIFKNSSGFPRLCLNASIFSEAEVLKNAKCMINTFRKYAESDKARRRFYNRCKMYRPIPCNKVLYKIDHRKEILVDLWSKSTNDRRREIIKTLYLKTFQTNETSLDFVEKV-KDNIMQLIINRDNESGDLYKEEREYPFSELLSDVGGLMGLWLGLSVIGLFEIVEFVY 495
[Close]
BLAST of Degenerin unc-8 vs. TrEMBL
Match: A0A5J4NUF1 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0013022 PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.405e-60
Identity = 151/554 (27.26%), Postives = 271/554 (48.92%), Query Frame = 1 
Query:   31 MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAP--IDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGN------PLSS---TQFVQITNNIPCYTYRIEN-LKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLP---IYKNE--SGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLF-EIRAKKECNKYLSKKCNENQYKIRISKE-----------NSPKFYFPCSNFENKY------ENFVC--------ENTTSQDLISK--AYGSDMVRLV-IKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVE-------ALAIIYRFLVNKLRIP 1533
            + +L+E+ T+HGL+H+    +K  + +W  L + GI G   N+  I  RYL+ P+L ++ S+   F WPD+T CN  AP  + L+G    +  W  +  + +  A+++ N++    +  Q      ++   L+ S+L   +  +     F   +   + + G+    T E         ++GN      PL     TQ +Q   N+PCYT ++ + L       ++SI + V+F+ + Y++ +  Y    + L+I++    P    I + PG    ++L   R+ +   R  CRT+ +T E++D  L++    +G    C + VSQ +++  C+CYSPFLP   +Y ++  S    +C+N + +S ++I  NA CM  IY +++ + ++  +   + C ++    C++ +YK+   +            N  +  F  S+F+N +      E  V         +NT   D + K   Y  D   L+ + R S+  +L+ EE EYP S L+SDVGGLMGLW+G+S IG+ E       A+     +LV+  R+P
Sbjct:   27 VERLLEHSTVHGLSHVSTAATKLGRITWFCLLLAGIAGFFTNLSFIIDRYLTRPVLTSYISEYEGFIWPDITLCNAMAPYSLHLHGR---IPKWQALRNQASAFAQKL-NSSLFASIDSQDQNEIAIQ--ALMYSTLSPPEYGVGEPEEFIQFVATDVGYQGIIL--TVE---------ISGNMTHLNPPLKKYFHTQVLQKRYNVPCYTLQLRHSLTAVQSNQVNSIGLGVKFNVESYRIINSSYASPYIDLFISHPGHAPHDDPIEMLPGYTLSLSLRMSRLRREPARLSCRTDFYTTEVFDPDLVTKRRVNGSATFCHRIVSQHLYLNSCHCYSPFLPYPVVYNHKLFSKMAILCFNMSRFSVAQINRNADCMLRIYERYQLSSVYNSVPEARACEQFRKPLCDDVEYKLSDIRRTAMVELWSNSYNEARIAFLTSSFQNLFSSDIPPEQLVSWLDSDTKDDNTKDNDSMPKILKYMRDNFGLLSVNRKSELGELVREELEYPLSELMSDVGGLMGLWIGISVIGLFEIVELCGFAIMTAVAWLVDLCRLP 563
[Close]
BLAST of Degenerin unc-8 vs. TrEMBL
Match: A0A183A5V1 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2336 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 1.692e-58
Identity = 144/537 (26.82%), Postives = 258/537 (48.04%), Query Frame = 1 
Query:   25 LLMNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFA-ETYEDFNLL------YFALVNGNPLSSTQFVQITNNIPCYTYRIEN-LKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNES-----GFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLF-EIRAKKECNKYLSKKCNENQYKIRISKENSP------------KFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVR--------LVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIG---VVEALAIIYRFLVNKL 1524
            L + +L+E+ T+HGL+H+    S+  +  W++L++ G+ G   N+  I  RY++ P+L ++  D  SF WPD+T CNP AP  ++    +   W  +      +A+ IS + +  +   +     I   + L+ S++   +  +   +     +      +G++   E Y  F  L      YF           Q +Q  +NIPCYT ++ + L   D    + +++ ++F++D + + +  Y    + L+I+     P+ + + L  G  +++++   R  +   R PCRT++F  +++D +L +     G    C K VSQ++++  C CYSPFLP    +      G P +C+N + ++ + I  NAKCMY++Y Q+  + ++  +   + C  + S    E+   +  S   +P            +  F  S FE+ +   + E    Q L +    S+M          + I R   R  L  EE EYP S LLSD+GGLMGLW+G+S IG   VVE LA +   +++ L
Sbjct:   28 LGVRRLLEHSTVHGLSHVSTASSRIGRSIWLILWLAGVAGFFTNLSFIVSRYMTRPVLTSYKHDHESFIWPDITLCNPMAPYPVHLRNRT-EKWNELRLRATRVAKNISEDLFAAIDDAEQLEIAI---QALLYSTISPEQFGVGEPQELIQFVAADKGRSGIHLTVEMYSTFTDLDAPKTQYF---------HAQMLQKRHNIPCYTLQLAHVLSQEDCLSTNQVSLGLKFNHDSFLIINSSYAPRNIDLFISLPGHFPEDESMELLSGYTHKVSVSMTRFIREPNRLPCRTDEFVAQMFDANLKTRREIVGSADFCHKLVSQRLYINNCGCYSPFLPYPVVDEHLLVHGQPVLCFNMSHFNITSIDQNAKCMYDVYTQYHDHSVYMNLPEAQYCETFRSTPPCESTSYMPESLRRTPIIELWSDTINEGRTAFLTSTFESVFGPDLPEERIRQLLNNSGNNSNMFTYMRNNFGMVTISRKFPRGTLTHEELEYPLSELLSDLGGLMGLWIGISVIGLFEVVELLAFVSLSMISAL 551
[Close]
BLAST of Degenerin unc-8 vs. TrEMBL
Match: A0A4E0RSD9 (Degenerin unc-8 OS=Fasciola hepatica OX=6192 GN=D915_000009 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 2.084e-58
Identity = 147/544 (27.02%), Postives = 261/544 (47.98%), Query Frame = 1 
Query:   25 LLMNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAP--IDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFA----ETYEDFNL---LYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISS--ITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFL---------PIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLF----------EIRAKKEC---NKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMV--------RLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIG---VVEALAIIYRFLVNKL 1524
            L + +L+E+ T+HGL+H+    ++  +  W++L++ G+ G   N+  I  RY++ P+L ++  D   F WPD+T CNP AP  + LN        W  + Q    ++R IS N +  + + +    T+   + LI S++   +   N      + +       G++       T+ + ++    YF +         Q +Q   NIPCYT R++ + L ++   S+  +++ ++F++D   + +  Y    + L+I+    +P+ + + +  G  +++T+   R  + + R PCRTE+F  +++D  L +     G    C K +SQ+++++ C CYSPFL         P+Y+     P +C+N + ++ S I  NA+CMY++Y QFK + ++            RA   C   N  L         +      N  +  F  S FE+ +   + E    Q + +    S ++         + I R   R  L  EE EYP S LLSDVGGLMGLW+G+S IG   VVE  A +   +++ L
Sbjct:   29 LGVRRLLEHSTVHGLSHVSTAGTRIGRSVWLILWMAGVAGFFTNLSFIVSRYMTRPVLTSYKHDHELFIWPDITLCNPMAPYPVHLNNRS---EKWNLLHQLATAVSRNISGNLFATIDEAEQEEVTV---QALIHSTVSPMEFGANDPHELVHFVAVDKGQTGIHLTIEMDATFTNLDIPKHRYFHV---------QVLQKRYNIPCYTLRLDQV-LNEEECASTKQVSLGLKFNHDSNLIINSSYAPFNIDLFISLPGHMPEDEPMEILSGYSHKVTVTMGRFTRERTRLPCRTEQFVTQVFDADLKTQREVVGSADFCLKLLSQRLYIDHCGCYSPFLPYPVMGQDHPLYQQ----PVLCFNMSHFNASGIDRNARCMYDVYTQFKEDFVYMNLPEAGYCETFRATPPCESINYVLESPRRTPIIEFWADTVNEARSAFVTSTFESVFGQDLPEERLKQMVNNSGNTSSLLFYMRNNFGLVKITRKLPRGTLTHEELEYPLSELLSDVGGLMGLWVGISVIGLFEVVELFAFVGLTVISAL 552
[Close]
BLAST of Degenerin unc-8 vs. TrEMBL
Match: A0A504YRR8 (Degenerin unc-8 OS=Fasciola gigantica OX=46835 GN=FGIG_05554 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 2.140e-58
Identity = 147/544 (27.02%), Postives = 261/544 (47.98%), Query Frame = 1 
Query:   25 LLMNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSA--PIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFA----ETYEDFNL---LYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISS--ITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFL---------PIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLF----------EIRAKKEC---NKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMV--------RLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIG---VVEALAIIYRFLVNKL 1524
            L + +L+E+ T+HGL+H+    ++  +  W++L++ G+ G   N+  I  RY++ P+L ++  D   F WPD+T CNP A  P+ LN        W  + Q    ++R IS N +  + + +    T+   + LI S++   +   N      + +       G++       T+ + ++    YF +         Q +Q   NIPCYT R++ + L ++   S+  +++ ++F++D   + +  Y    + L+I+    +P+ + + +  G  +++T+   R  + + R PCRTE+F  +++D  L +     G    C K +SQ+++++ C CYSPFL         P+Y++    P +C+N + ++ S I  NA+CMY +Y QFK + ++            RA   C   N  L         +      N  +  F  S FE+ +   + E    Q + +    S ++         + I R   R  L  EE EYP S LLSDVGGLMGLW+G+S IG   VVE  A +   +++ L
Sbjct:   29 LGVRRLLEHSTVHGLSHVSTASTRIGRSVWLILWMAGVAGFFTNLSFIVSRYMTRPVLTSYKHDHELFIWPDITLCNPMAPYPVHLNNRS---EKWNLLHQLATAVSRNISGNLFATIDEAEQEEVTV---QALIHSTVSPMEFGANDPHELVHFVAVDKGQTGIHLTIEMDATFTNLDIPKHRYFHV---------QVLQKRYNIPCYTLRLDQV-LNEEECASTKQVSLGLKFNHDSNLIINSSYAPFNIDLFISLPGHMPEDEPMEILSGYSHKVTVTMGRFTRERTRLPCRTEQFVTQVFDADLKTQREVVGSADFCLKLLSQRLYIDHCGCYSPFLPYPVMGQDHPLYQH----PVLCFNMSHFNASGIDRNARCMYEVYTQFKEDFIYMNLPEAGFCETFRATPPCESINYVLESPRRTPIIEFWADTVNEARSAFVTSTFESVFGQDLPEERLKQMVNNSGNTSSLLFYMRNNFGLVKITRKLPRGTLTHEELEYPLSELLSDVGGLMGLWVGISVIGLFEVVELFAFVGLTVISAL 552
[Close]
BLAST of Degenerin unc-8 vs. Ensembl Nematostella
Match: EDO31696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX71])

HSP 1 Score: 48.1358 bits (113), Expect = 6.230e-6
Identity = 63/274 (22.99%), Postives = 107/274 (39.05%), Query Frame = 1 
Query:  775 LPQKKEIILQPGNDYEITLEQFRIEKLKKRN--PCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDL-ISKAYGSDMVRLVIKRYSKRKDLLLEEE-EYPFSNLLSDVGGLMGLWLGMSFIGVVE----ALAIIYRFLVNKLRIPRNPSQNEENNFQP 1572
             P+++  ++ PG   +I LE+  I+++   N   C T++ +I   +I     N             S+   V  C  Y         E  FP      T  S        +C+  +  +  RN+L        C+      C E  Y++  S    P           +Y+N + +N        S+++  D   L +  + K  +  +EE   Y   N LSD+GG +GLW+G+S +   E    AL I ++    K   PR  + N E + +P
Sbjct:  177 FPEERGTLVAPGFSTDIGLEKMDIKRVDPHNNGSCVTDEASINTENIFKQKFN----------TSYSKTACVLSCLAYHQMHECKCMEYRFPTDSVCDTLNSTI-----VECLAKVKFKLSRNEL-------GCSTACGPACREEAYRLTSSFAVWPS---------QRYQNSIIDNLFDGIFNYSRSWFRDNY-LEVNIFFKELNYFIEESVAYELVNFLSDIGGQLGLWVGVSVMTGAEVVELALLIFFKVFCRK---PRKVTVNSEGDPEP 415
[Close]
BLAST of Degenerin unc-8 vs. Planmine SMEST
Match: SMESG000073267.1 (SMESG000073267.1)

HSP 1 Score: 1061.98 bits (2745), Expect = 0.000e+0
Identity = 520/522 (99.62%), Postives = 521/522 (99.81%), Query Frame = 1 
Query:   31 MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVNKLRIPRNPSQNEENNFQPTEMLIMNN 1596
            MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKE SPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVNKLRIPRNPSQNEENN+QPTEMLIMNN
Sbjct:    1 MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKEKSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVNKLRIPRNPSQNEENNYQPTEMLIMNN 522
[Close]
BLAST of Degenerin unc-8 vs. Planmine SMEST
Match: SMESG000069075.1 (SMESG000069075.1)

HSP 1 Score: 362.844 bits (930), Expect = 2.603e-119
Identity = 200/498 (40.16%), Postives = 297/498 (59.64%), Query Frame = 1 
Query:   40 LIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENF------VCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLV 1515
            LI++ TIHG+ HIY   +K  K  WI+LFVFGI GI +NI++IS+RY S+P+LI+H  D   F WPDLTFCNP AP  L  +P + +YW  ++    +    + +    E  K +  G++    R + +S+++  K+K+N++   F  I Y  +H+G+YF++ YE  N   F   + + L +TQ VQI NNIPC T+ ++  KL D   I ++   + FD + Y++F+  YV   + LYITN +ELP   EIIL PG +  I LEQ R E    +  CR E +T+ELYD  L SV L++G + DC+K VSQ+++V  C CYSPFLPIYK+E   P++C NAT ++ S++L N++CM  IYN++    +F     + C+ Y   KCN  + K    KE   + +   SN E +Y         + +   + D   +    +++++ +KR +   DL  EE EYPFS LLSDVGGLMGLWLGMS +G+ E    +Y F++
Sbjct:    7 LIQSSTIHGIGHIYSATNKLWKCFWILLFVFGIGGIIVNILVISIRYSSSPVLISHIIDYERFIWPDLTFCNPGAPFTLTKDPDTTAYWYKVNNRSTQAENFLKSTEIFE--KIENKGNSGARERAVTMSTVNPNKMKVNSNYNEFLFIGYIKAHSGIYFSKEYEGNNPTSFQ-DDIDELFTTQLVQIQNNIPCQTFHVDKSKL-DPYSIETLVFYIIFDQESYRVFNSSYVVRSLILYITNTHELPSS-EIILIPGFNNIIELEQSRYEFHGIKRRCRNENYTVELYDAMLQSVTLFEGSFEDCKKVVSQELYVNNCYCYSPFLPIYKDEKFPPQLCLNATLFTESQLLINSRCMVKIYNEYHDESIFSKIINERCSNYKHDKCNIVKLKTDQRKEMMIELWSKSSN-ERRYNELKGLHRQILKYNYTGDGFIEQIRENLIKITVKRRTPFGDLFREENEYPFSQLLSDVGGLMGLWLGMSVVGLFEIFEFLYHFII 498
[Close]
BLAST of Degenerin unc-8 vs. Planmine SMEST
Match: SMESG000069074.1 (SMESG000069074.1)

HSP 1 Score: 362.459 bits (929), Expect = 3.595e-119
Identity = 204/502 (40.64%), Postives = 299/502 (59.56%), Query Frame = 1 
Query:   40 LIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYLEVMK--PQIA--GSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSNFENKYENF------VCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLV 1515
            LI++ TIHG+ HIY   +K  K  WI+LFVFGI GI +NI++IS+RY S+P+LI+H  D   F WPDLTFCNP AP  L  +P + +YW  ++       R     N+L+      +IA  G++    R + +S+++  K+K+N++   F  I Y  +H+G+YF++ YE  N   F   + + L +TQ VQI NNIPC T+ ++  KL D   I ++   + FD + Y++F+  YV   + LYITN +ELP   EIIL PG +  I LEQ R E    +  CR E +T+ELYD  L SV L++G + DC+K VSQ+++V  C CYSPFLPIYK+E   PR+C NAT ++ S++L N++CM  IYN++    +F     + C+ Y   KCN  + K    KE   + +   SN E +Y         + +   + D   +    +++++ +KR +   DL  EE EYPFS LLSDVGGLMGLWLGMS +G+ E    +Y F++
Sbjct:    7 LIQSSTIHGIGHIYSATNKLWKCFWILLFVFGIGGIIVNILVISIRYSSSPVLISHIIDYERFIWPDLTFCNPGAPFTLTKDPDTTAYWYKVNN------RSTQAENFLKSTGIFKEIANEGNSGARERAVTMSTVNPNKIKVNSNYNEFLFIGYIKAHSGIYFSKEYEGNNPTSFQ-DDIDELFTTQLVQIQNNIPCQTFHVDKSKL-DPYSIETLVFYIIFDQESYRVFNSSYVVRSLILYITNTHELPSS-EIILIPGFNNIIELEQSRYEFHGIKRRCRNENYTVELYDAMLQSVTLFEGSFEDCKKVVSQELYVNNCYCYSPFLPIYKDEKFPPRLCLNATLFTESQLLINSRCMVKIYNEYHDESIFSKIINERCSNYKHDKCNIVKLKTDQRKEMMIELWSKSSN-ERRYNELKGLHRQILKYNYTGDGFIEQIRENLIKITVKRKTPFGDLFKEENEYPFSQLLSDVGGLMGLWLGMSVVGLFEIFEFLYHFII 498
[Close]
BLAST of Degenerin unc-8 vs. Planmine SMEST
Match: SMESG000017678.1 (SMESG000017678.1)

HSP 1 Score: 362.459 bits (929), Expect = 3.869e-119
Identity = 198/502 (39.44%), Postives = 290/502 (57.77%), Query Frame = 1 
Query:   31 MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNN-YLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSN-----FENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVN 1518
            M +++++ TIHG++HI+  KS+  K  WI LF+FGI GI +NIV + ++Y SNP+LI+H  D   F WPD+TFC P+AP        + ++W ++      +   ++N     +++  +      LE RT+ +SS D  KLK N  R     I+++    G+Y+        +  FA  +  PL +   VQI NNIPC+T+ I+  K  +   I SI+  ++F+ + Y LF+  Y++  VFLYITN+N +P K EI+L PG +  ++L Q R E       CR + F  ELYD  L S + ++G Y DC+K  SQK+FV  C CYSPFLPIYKNE+G PRIC NATFYS  EI+ NAKCM ++Y +F+    F    + +C  Y   KCNE +YKI   KE   +F+   +N        K   ++ E    ++   +    + + + I R  ++ DL  EE EYPFS LLSD+GGLMGLWLGMS +G+ E   ++Y +L N
Sbjct:    1 MIEILQSSTIHGVSHIHGAKSQITKVLWIFLFIFGIIGIFVNIVFVIIKYFSNPVLISHKLDHEPFIWPDVTFCKPTAPYYFGENSQAYTHWSHVFWRTRRIVERLANETEVFKIINGKRNTDYELEERTIAMSSADPYKLKTNDFRYHIMAIYFNKMQRGIYYHIDNSGIAVTSFA-EDLKPLFTAPLVQIRNNIPCFTFHIDKSKFLNQNQIKSISFYLKFNLESYLLFNSSYLHRSVFLYITNRNGIPHKNEILLAPGFNNIVSLSQSRYEYRGVLRKCRNKIFETELYDGLLQSTSTFEGHYDDCKKVESQKLFVGICKCYSPFLPIYKNETGPPRICLNATFYSEDEIVRNAKCMTDVYTKFQSYKAFRNHYRLKCQDYRFIKCNEVKYKIEKDKELFVEFWSGLTNQRRNELLKKVSYYLMEKFDIKNYFERM-RDNFIGITINREFQKGDLYKEEYEYPFSELLSDIGGLMGLWLGMSVVGLFEIFELMYLYLKN 500
[Close]
BLAST of Degenerin unc-8 vs. Planmine SMEST
Match: SMESG000069054.1 (SMESG000069054.1)

HSP 1 Score: 355.91 bits (912), Expect = 1.277e-116
Identity = 196/525 (37.33%), Postives = 311/525 (59.24%), Query Frame = 1 
Query:   31 MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRYLSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEINELAREISNNNYL-EVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFNLIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTYRIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELPQKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNLYDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRSEILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKENSPKFYFPCSN--------FENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRKDLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVNKLRIPRNPSQNEENNFQPTE 1578
            M +L+++ TIHG+NHIY    K AKF W VLF+FGI GI +NI  +S+RY S P+LI+++ +   F WPD+TFCNP+AP  L+ +  +  YW+ ++ +  ++ + +     L ++++        LE RT+ ++S +  KL  +        + Y+  H+G+YF +  ++ N   F   +  PL STQ VQI NNIPC+T+ +    + +   + S+   +RF+YD Y++F+  Y    +FLYI+N++E+ Q + I L PG +  ++L Q R      R  CR + F  E+YD    +V  ++G Y DC++ VSQ++FV+KC CYSPFLPI+KN++GFPR+C NATF+S++++L+NAKCM N+Y  FK++  F      +C  +   KC+   Y++ + K+   + +    N        F + Y  +  +N + +D  ++    ++V L+I+R  +  DL  EE EYPFS LLSD+GGLMGLWLGMS +G+ E L   Y F +N     +N S+    N Q  E
Sbjct:    1 MIELVKSSTIHGINHIYSANGKLAKFLWCVLFIFGIIGIIVNICFVSIRYFSYPVLISYTFEHEPFVWPDITFCNPTAPFYLDRKSKAYKYWMQINNQSRDVVKMLVKETKLFQIIRESKETDFELEERTIAMASSNPVKLGADLFENHVKFVGYTRPHSGIYFNKDIQNNNPKTFP-GDLKPLFSTQIVQIQNNIPCHTFHMHKSNILNPNSVQSLVFGLRFNYDSYEIFNSSYTTRSIFLYISNRDEIQQDQNIFLSPGFNNILSLSQNRHHYNGLRRKCRHKTFKFEVYDALFQNVKTFEGSYEDCKQIVSQRLFVDKCKCYSPFLPIFKNQTGFPRLCLNATFFSKTKLLENAKCMVNVYENFKKSGNFRKHYNNQCLIFKLHKCDLLSYQMDLRKDMLVELWSKVYNGKRLNSIKFMHSY--YFGKNESKKDF-NEVMRDNIVDLIIERKFEYSDLFKEEREYPFSELLSDIGGLMGLWLGMSVVGLFEILEFFYLFSINACLNKKNKSRFSPKNIQFDE 521
The following BLAST results are available for this feature:
BLAST of Degenerin unc-8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-1054.147e-721.86Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1054.147e-721.86Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1054.166e-721.86Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1054.166e-721.86Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
unc-1054.452e-721.86Degenerin-like protein unc-105 [Source:UniProtKB/... [more]
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BLAST of Degenerin unc-8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ASIC18.903e-621.07acid sensing ion channel subunit 1 [Source:NCBI ge... [more]
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BLAST of Degenerin unc-8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q09274|UN105_CAEEL5.300e-621.86Degenerin-like protein unc-105 OS=Caenorhabditis e... [more]
sp|O01635|ASIC1_CAEEL7.369e-624.26Degenerin-like protein asic-1 OS=Caenorhabditis el... [more]
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BLAST of Degenerin unc-8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
S5W2R22.667e-10037.60Asic OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
A0A5J4NUF11.405e-6027.26Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A183A5V11.692e-5826.82Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A4E0RSD92.084e-5827.02Degenerin unc-8 OS=Fasciola hepatica OX=6192 GN=D9... [more]
A0A504YRR82.140e-5827.02Degenerin unc-8 OS=Fasciola gigantica OX=46835 GN=... [more]
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BLAST of Degenerin unc-8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO316966.230e-622.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Degenerin unc-8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Degenerin unc-8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000073267.10.000e+099.62SMESG000073267.1[more]
SMESG000069075.12.603e-11940.16SMESG000069075.1[more]
SMESG000069074.13.595e-11940.64SMESG000069074.1[more]
SMESG000017678.13.869e-11939.44SMESG000017678.1[more]
SMESG000069054.11.277e-11637.33SMESG000069054.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007660 ID=SMED30007660|Name=Degenerin unc-8|organism=Schmidtea mediterranea sexual|type=transcript|length=1681bp
TAAGAATTAAATTTCTCATCAAAGTTACTCATGAATAAACTAATTGAAAA
TTGCACAATTCATGGCTTGAATCACATTTATTTGGAGAAAAGTAAACCGG
CCAAATTTTCTTGGATTGTTTTATTTGTCTTTGGGATTTGTGGGATAACT
TTGAATATTGTTTTAATCTCGTTGAGATACTTATCAAATCCCATTTTAAT
TAATCACAGCTCCGACAGTTTCTCTTTTCAGTGGCCTGATTTGACATTCT
GCAATCCATCCGCTCCAATTGATTTGAATGGTGAACCTTTGTCGTTGAGT
TACTGGCTGAATATGTCCCAGGAAATTAACGAATTAGCTAGAGAGATCAG
TAACAACAATTATTTGGAAGTTATGAAGCCTCAAATTGCAGGAAGCACAA
TTTTAGAGAGAAGAACTCTCATCTTATCCAGCCTTGATAATGCTAAATTA
AAGTTAAATACATCTCGACGATTTTTTAACTTGATTCACTATTCTATTTC
CCATAATGGAATGTATTTCGCTGAAACCTATGAAGATTTTAACTTACTTT
ATTTTGCTCTAGTAAATGGAAATCCTTTATCTTCGACGCAATTCGTCCAG
ATTACCAACAACATTCCTTGTTATACTTATCGAATTGAAAACTTAAAGCT
TACGGACGATCGGGATATAAGTTCCATAACAATGCGCGTTCGATTCGACT
ATGACAAATATCAACTTTTCGATGAGCAATATGTTTATGGTGGAGTTTTC
CTCTACATAACAAATAAAAATGAATTGCCTCAGAAAAAGGAGATTATTTT
ACAACCCGGAAATGACTATGAAATCACATTGGAGCAATTTCGAATCGAAA
AACTAAAAAAGAGAAACCCCTGTCGAACTGAAAAGTTTACAATAGAACTA
TACGATATATCGCTATTGTCGGTGAATCTTTACGATGGCGGCTACATAGA
CTGTGAAAAATATGTCAGTCAAAAGATATTCGTTGAAAAATGTAATTGCT
ACAGTCCTTTTCTGCCAATTTATAAGAATGAATCAGGATTTCCTCGAATC
TGTTACAATGCCACTTTTTATTCCAGATCTGAAATTCTTGACAATGCAAA
ATGCATGTACAATATTTACAATCAATTTAAAAGAAATGATTTGTTTGAAA
TAAGAGCCAAAAAAGAATGCAATAAATATTTATCTAAAAAATGTAATGAA
AATCAGTATAAAATAAGAATTTCAAAAGAAAATTCTCCAAAATTTTATTT
TCCCTGTTCAAATTTTGAAAACAAATACGAGAATTTTGTTTGTGAAAATA
CAACGTCACAAGATTTGATAAGTAAAGCATATGGGAGTGATATGGTGAGA
CTCGTAATCAAGCGGTATTCAAAAAGGAAAGATTTATTACTAGAGGAAGA
AGAATATCCGTTTTCAAATTTATTGTCGGACGTTGGGGGTTTGATGGGAC
TTTGGCTGGGCATGAGTTTCATCGGAGTAGTTGAAGCTTTAGCAATTATC
TATCGGTTTCTTGTGAACAAACTTAGGATCCCTAGAAATCCCTCTCAAAA
TGAGGAAAACAATTTCCAACCTACCGAAATGTTGATCATGAATAATTAAC
CTTTACTTTTTTTATTAGTACTATTATAAGATTTTATTTTCTATTTCCTC
ATTACGAAATAAAAATGCTTAAATTACAAAA
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protein sequence of SMED30007660-orf-1

>SMED30007660-orf-1 ID=SMED30007660-orf-1|Name=SMED30007660-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=523bp
MNKLIENCTIHGLNHIYLEKSKPAKFSWIVLFVFGICGITLNIVLISLRY
LSNPILINHSSDSFSFQWPDLTFCNPSAPIDLNGEPLSLSYWLNMSQEIN
ELAREISNNNYLEVMKPQIAGSTILERRTLILSSLDNAKLKLNTSRRFFN
LIHYSISHNGMYFAETYEDFNLLYFALVNGNPLSSTQFVQITNNIPCYTY
RIENLKLTDDRDISSITMRVRFDYDKYQLFDEQYVYGGVFLYITNKNELP
QKKEIILQPGNDYEITLEQFRIEKLKKRNPCRTEKFTIELYDISLLSVNL
YDGGYIDCEKYVSQKIFVEKCNCYSPFLPIYKNESGFPRICYNATFYSRS
EILDNAKCMYNIYNQFKRNDLFEIRAKKECNKYLSKKCNENQYKIRISKE
NSPKFYFPCSNFENKYENFVCENTTSQDLISKAYGSDMVRLVIKRYSKRK
DLLLEEEEYPFSNLLSDVGGLMGLWLGMSFIGVVEALAIIYRFLVNKLRI
PRNPSQNEENNFQPTEMLIMNN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
Vocabulary: biological process
TermDefinition
GO:0006814sodium ion transport
GO:0006810transport
GO:0006811ion transport
GO:0035725sodium ion transmembrane transport
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPRINTSPR01078AMINACHANNELcoord: 464..478
score: 41.07
coord: 323..340
score: 31.33
coord: 478..494
score: 48.94
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 15..496
e-value: 7.3E-43
score: 147.1
IPR001873Epithelial sodium channelPANTHERPTHR11690AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATEDcoord: 14..519
NoneNo IPR availableGENE3DG3DSA:1.10.287.820coord: 283..397
e-value: 9.2E-23
score: 82.8
NoneNo IPR availableGENE3DG3DSA:1.10.3590.10coord: 86..247
e-value: 9.2E-23
score: 82.8
NoneNo IPR availableGENE3DG3DSA:1.10.287.770coord: 35..494
e-value: 9.2E-23
score: 82.8
NoneNo IPR availableGENE3DG3DSA:2.60.470.10coord: 64..466
e-value: 9.2E-23
score: 82.8
NoneNo IPR availableTMHMMTMhelixcoord: 35..57
NoneNo IPR availableTMHMMTMhelixcoord: 482..504