SMED30007586

Overview
NameSMED30007586
Smed IDSMED30007586
Length (bp)1482
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007586 (SMED30007586) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007586 (SMED30007586) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007586 (SMED30007586) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007586 (SMED30007586) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30007586

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30007586h1SMcG0020291 Contig50305newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007586h1SMcG0020291 Contig50305uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30007586h1SMcG0020291 dd_Smed_v4_546_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007586h1SMcG0020291 dd_Smed_v4_546_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30007586h1SMcG0020291 dd_Smed_v4_546_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007586h1SMcG0020291 dd_Smed_v4_546_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30007586h1SMcG0020291 OX_Smed_1.0.07725ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007586 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000939:57846..60332 +7163
Homology
[Close]
BLAST of SMED30007586 vs. Ensembl Xenopus
Match: ephx2 (epoxide hydrolase 2, cytoplasmic [Source:Xenbase;Acc:XB-GENE-947544])

HSP 1 Score: 49.2914 bits (116), Expect = 6.226e-6
Identity = 39/147 (26.53%), Postives = 68/147 (46.26%), Query Frame = 2 
Query:  599 KVKSTVISHLKKEFINKYGIDA-SYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRRINRDILY 1036
            KV+  V + +   F    GI   S +R  +  Q +  CCGIDN  D+++T W++   N       VPLSCC     +++     + ++P D    +Y++GC  ++   +   + +   A      I L  ++ +CI+  R  RD  Y
Sbjct:  110 KVEDEVDNTIANVFNQYNGISPDSASRAIDYVQRQLHCCGIDNYLDWENTPWFSEAKNN-----SVPLSCC----RNYVFNCTGSMNKPGD----LYSEGCKALVVEKLQEIMMYVIWAALAFAAIQLLGMLCACIVLCRRTRDPAY 243
[Close]
BLAST of SMED30007586 vs. TrEMBL
Match: A0A4E0RVG9 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_003895 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.066e-8
Identity = 30/107 (28.04%), Postives = 56/107 (52.34%), Query Frame = 2 
Query:  599 KVKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPK-EGEVPLSCCTMED-EHFINTTACAHSRPEDQSKYIYNKGCWDILR 913
            +++ ++ + ++  F   YG D + T  W+  Q  + CCG+     Y S++W   P+N  P+  G VP SCC   D + ++N   C  S  ++   ++Y  GC++ +R
Sbjct:  177 QIRDSLRNEMEHRFTMNYGFDLALTNAWDSTQWSYHCCGVAGPETYASSRWIRDPNNLSPRSSGLVPDSCCKRYDNQTYVNLDGCYISPLQEAEPFLYVHGCYEFIR 283
[Close]
BLAST of SMED30007586 vs. TrEMBL
Match: A0A443RHJ7 (Tetraspanin-1-like protein OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_11527 PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.197e-8
Identity = 49/157 (31.21%), Postives = 72/157 (45.86%), Query Frame = 2 
Query:  602 VKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGE-VPLSCCTMEDEHFINTT-ACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVII------FSCIMNRRINRDILYGQVV 1048
            + + + S LK    N+YG D  +  +W++ Q ++ CCG+D   DY  T W     ++F      VP SCC M     I    +C  S  ED+   IY  GC+D +       L+W   +  ILC +L F +I      F CI+   I   I   QV+
Sbjct:   58 IATNLRSDLKNRLNNEYGFDYIFQTIWDKIQSENQCCGVDGPYDYNMTLWLAKVHSDFSSNAMLVPNSCCRMSSNKNIPLDYSCVDSYSEDK---IYTFGCYDSI-------LHWLQRSANILC-VLGFCVISFLKLCFVCILRYEIKEMIQKIQVL 203
[Close]
BLAST of SMED30007586 vs. TrEMBL
Match: A0A3S4RD86 (Tetraspanin-1-like protein (Fragment) OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_03618 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.111e-8
Identity = 49/157 (31.21%), Postives = 72/157 (45.86%), Query Frame = 2 
Query:  602 VKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGE-VPLSCCTMEDEHFINTT-ACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVII------FSCIMNRRINRDILYGQVV 1048
            + + + S LK    N+YG D  +  +W++ Q ++ CCG+D   DY  T W     ++F      VP SCC M     I    +C  S  ED+   IY  GC+D +       L+W   +  ILC +L F +I      F CI+   I   I   QV+
Sbjct:  106 IATNLRSDLKNRLNNEYGFDYIFQTIWDKIQSENQCCGVDGPYDYNMTLWLAKVHSDFSSNAMLVPNSCCRMSSNKNIPLDYSCVDSYSEDK---IYTFGCYDSI-------LHWLQRSANILC-VLGFCVISFLKLCFVCILRYEIKEMIQKIQVL 251
[Close]
BLAST of SMED30007586 vs. TrEMBL
Match: A0A3M6V1N1 (Tetraspanin OS=Pocillopora damicornis OX=46731 GN=pdam_00012077 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.031e-7
Identity = 44/138 (31.88%), Postives = 68/138 (49.28%), Query Frame = 2 
Query:  599 KVKSTVISHLKKEFINKYG--IDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIM 1006
            +V++ V + LK+E    YG  ID++  +  +  Q +  CCG  N  D+  +KW      E  K+ +VPLSCC  ++    NT  C      D+ K I+ KGC   L + V   L+   I    + GI L  +IF+C +
Sbjct:  108 RVETEVENRLKEEIKTNYGVKIDSTTNQNVDNLQIRLECCGASNYTDWDESKW-----KEVNKDNKVPLSCCKKDE----NTRTCNKVSNFDKKK-IHTKGCLQSLTDFVDKHLFVLGIVAVSIAGIQLLGMIFACCL 235
[Close]
BLAST of SMED30007586 vs. TrEMBL
Match: A0A267EA85 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006964g2 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 6.374e-7
Identity = 35/118 (29.66%), Postives = 63/118 (53.39%), Query Frame = 2 
Query:  671 TRLWNEFQPKHMCCGIDNGADY-KSTKW--YNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRR 1015
            T LWN F     CCGI+NG+D+  ST W  Y + S ++      PL+CC +      N TAC H+   D +   +N GC++++  VV + + ++++    +  + + ++ FS  + R+
Sbjct:  161 TVLWNVFHMSLSCCGINNGSDFLSSTTWPRYVNYSGKYYDSVAYPLTCCKLT-----NWTACLHTNHTDAAYSNWNIGCYNLIDRVVQINIVYAALGTAAVLLLQVLILDFSVHLCRK 273
[Close]
BLAST of SMED30007586 vs. Ensembl Cavefish
Match: zgc:113223 (tetraspanin-33-like [Source:NCBI gene;Acc:103041228])

HSP 1 Score: 48.9062 bits (115), Expect = 4.352e-6
Identity = 28/98 (28.57%), Postives = 45/98 (45.92%), Query Frame = 2 
Query:  623 HLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYN-SPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQ---SKYIYNKGCWD 904
             L K+ I  Y  D     + +  Q K +CCG+D+  D+    ++N S  N   +   VP SCC  +    +  + C +    D+   S+ IY KGC +
Sbjct:  112 QLMKKAIVLYREDLDLENVIDFVQKKFLCCGVDDYTDWSQNVYFNCSEKNPSLEACGVPFSCCVRQPNETVFNSMCGYETQRDESSPSERIYTKGCLE 209
[Close]
BLAST of SMED30007586 vs. Ensembl Cavefish
Match: zgc:113223 (tetraspanin-33-like [Source:NCBI gene;Acc:103041228])

HSP 1 Score: 48.9062 bits (115), Expect = 4.714e-6
Identity = 28/98 (28.57%), Postives = 45/98 (45.92%), Query Frame = 2 
Query:  623 HLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYN-SPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQ---SKYIYNKGCWD 904
             L K+ I  Y  D     + +  Q K +CCG+D+  D+    ++N S  N   +   VP SCC  +    +  + C +    D+   S+ IY KGC +
Sbjct:  109 QLMKKAIVLYREDLDLENVIDFVQKKFLCCGVDDYTDWSQNVYFNCSEKNPSLEACGVPFSCCVRQPNETVFNSMCGYETQRDESSPSERIYTKGCLE 206
[Close]
BLAST of SMED30007586 vs. Ensembl Nematostella
Match: EDO35938 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK25])

HSP 1 Score: 51.6026 bits (122), Expect = 1.786e-7
Identity = 39/143 (27.27%), Postives = 63/143 (44.06%), Query Frame = 2 
Query:  599 KVKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRRINRD 1027
            +V+  +  +L ++  N YG D+      N  Q +  CCG +   D+  +KW      +  K+  VPLSCCT       N T C     +D  K +Y+ GC + L+  V   +         +  + L  +IFSC +   I+ D
Sbjct:  114 QVQEEIKINLDEQIRNTYGYDSEVESHINNLQIRLKCCGGERPGDWLGSKW----KRDLNKDKIVPLSCCTENA----NKTTC---YKDDTYKEVYSSGCVNELKTFVDKHMILIGAIAVGISIVQLLGMIFSCCLFCAIDND 245
[Close]
BLAST of SMED30007586 vs. Ensembl Medaka
Match: cd63 (CD63 molecule [Source:NCBI gene;Acc:101174188])

HSP 1 Score: 49.2914 bits (116), Expect = 2.971e-6
Identity = 27/112 (24.11%), Postives = 53/112 (47.32%), Query Frame = 2 
Query:  683 NEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRRI 1018
            ++ Q    CCG+++ +D+    + +S  N       VP SCC       IN T          +  +Y KGC D++ N++   + W  +A  ++  + +  ++FSC++ + I
Sbjct:  138 DQIQENLKCCGVNSSSDWS---YLDSDGNS------VPDSCC-------INKTKGCGKGSMKNAGTVYQKGCHDVIENLLKTNIQWVIVAAIVIALVQILGVVFSCLLMKSI 233
[Close]
BLAST of SMED30007586 vs. Ensembl Medaka
Match: tspan14 (tetraspanin-14 [Source:NCBI gene;Acc:101174770])

HSP 1 Score: 49.6766 bits (117), Expect = 3.383e-6
Identity = 33/122 (27.05%), Postives = 56/122 (45.90%), Query Frame = 2 
Query:  641 INKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYN-SPSNEFPKEGEVPLSCCTMEDEHFINTTACAH---SRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIF 994
            I  Y  D     L +  Q  + CCG  +  D+    ++N + +N   +   VP SCC  +    +  T C +    + +D + YI+ KGC   L + +   LY  +I VF++  +L  V I+
Sbjct:  199 IRSYRDDIDLQNLIDSLQKMNQCCGAKDPNDWDENLYFNCNMTNRSRESCGVPFSCCISDPTDLVINTQCGYDVRKKKDDWANYIHTKGCITALEDWLPRNLYTVAI-VFVVISLLQMVGIY 319
[Close]
BLAST of SMED30007586 vs. Planmine SMEST
Match: SMESG000043582.1 (SMESG000043582.1)

HSP 1 Score: 314.694 bits (805), Expect = 3.043e-105
Identity = 147/150 (98.00%), Postives = 150/150 (100.00%), Query Frame = 2 
Query:  599 KVKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRRINRDILYGQVV 1048
            +VKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTM+DEHF+NTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRRINRDILYGQVV
Sbjct:  105 QVKSTVISHLKKEFINKYGIDASYTRLWNEFQPKHMCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMKDEHFLNTTACAHSRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCIMNRRINRDILYGQVV 254
The following BLAST results are available for this feature:
BLAST of SMED30007586 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ephx26.226e-626.53epoxide hydrolase 2, cytoplasmic [Source:Xenbase;A... [more]
back to top
BLAST of SMED30007586 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RVG91.066e-828.04Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A443RHJ72.197e-831.21Tetraspanin-1-like protein OS=Dinothrombium tincto... [more]
A0A3S4RD864.111e-831.21Tetraspanin-1-like protein (Fragment) OS=Dinothrom... [more]
A0A3M6V1N11.031e-731.88Tetraspanin OS=Pocillopora damicornis OX=46731 GN=... [more]
A0A267EA856.374e-729.66Uncharacterized protein OS=Macrostomum lignano OX=... [more]
back to top
BLAST of SMED30007586 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zgc:1132234.352e-628.57tetraspanin-33-like [Source:NCBI gene;Acc:10304122... [more]
zgc:1132234.714e-628.57tetraspanin-33-like [Source:NCBI gene;Acc:10304122... [more]
back to top
BLAST of SMED30007586 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30007586 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO359381.786e-727.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30007586 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cd632.971e-624.11CD63 molecule [Source:NCBI gene;Acc:101174188][more]
tspan143.383e-627.05tetraspanin-14 [Source:NCBI gene;Acc:101174770][more]
back to top
BLAST of SMED30007586 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000043582.13.043e-10598.00SMESG000043582.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007586 ID=SMED30007586|Name=SMED30007586|organism=Schmidtea mediterranea sexual|type=transcript|length=1482bp
GTGTTATGTGAATTGTTCCATTTACGGAAAATCAAAATTTCGATCACTAG
ATGCTTGAATTGGGTTCATTTAGACCTCTTGCACCCATAATTTTTGTTTT
CTCTTTCATGGACCCCTTGCATTCTAGAGTAGACCCATAAGGATCGGCAT
GGGAATCATTGGAATAGATCTTCGTTTTCAGGTAGAATTTGACATAACAT
GTATATCTATTGGCTGATTGCGGGTTTCTAGTTTAAACTTGAGTTCTGAT
TGATTCGCAATATACTTTGTATTATTTCGATTAATTGAGTTTGAAGCAGC
AATAAACTTTATATCTAAAACATTAGCAGTTGAGTTCATGAGTGTCTTTT
CAATATTATTTAATCAATTATTTAATTTATTTAAACGGTGTACAAACAAA
AGTTTTAATGAGGTGGTAACAATATTGAATTTTTGTTTAAATATACAATT
CGATAGGTGCTATTGATAGCACACGGAAATATAAATTACTTAAAAGAGGA
TTTAAGGTGGATTTTTTAGCGATATATCAGTATTTCTAGGAAAGGAGAGA
TTTTTTTGAACTAGTTGAATTTGAGTTTATTTATCATGCTAATGTGTGAA
GGTCAAGAGCACGGTCATCAGTCATTTGAAAAAGGAATTCATCAATAAAT
ACGGAATTGACGCTAGTTACACAAGGTTATGGAATGAATTCCAACCGAAG
CACATGTGTTGCGGAATTGATAACGGCGCTGATTATAAGTCTACAAAATG
GTACAACTCACCATCAAATGAGTTTCCGAAAGAAGGTGAGGTGCCGTTAT
CCTGCTGTACAATGGAAGACGAGCACTTCATTAATACCACAGCTTGCGCA
CATTCAAGGCCAGAAGATCAAAGCAAATATATATATAACAAAGGATGCTG
GGACATTTTACGAAATGTCGTATCGCTAACACTGTATTGGTCATCGATTG
CCGTATTCATTTTATGTGGCATCTTACTGTTTGTCATCATATTCAGTTGC
ATCATGAACAGAAGAATTAATAGAGACATACTTTACGGCCAAGTGGTGTA
GTGATTCGGGAGCTCTGGAGCATTTCATGCGAAACATTGTATAACACTGT
TTATCATCCTTTTATTATTTTGTCATAAATATATTATTATAATGAAACTT
TGTCGTTATTTTTTGCCAATAATATTTAATTGTAATTGCGTTACTAAAAT
TTATGTAATTATTTCGTGTCTATTTTACGCGGTGGAATTTGTCAATAATG
AAAAACCGGGTGTATTTCTTGAATGAAATTATTTTGTTTAAATGAGAGGT
GATGTTATAAATATTATTATTGTGTTTGAATGGGCTATATTTTTTCCAAT
GATTTGATTTATTTTTTTGTTTAAATGTATTGATCTAACATTTTTTAATT
TTTATTATGTTATATAATAATATTGTGTGATTTATATTTTTAACGTGATA
CTAAAATTAATTAATTTAAATTTTATCGTGTC
back to top

protein sequence of SMED30007586-orf-1

>SMED30007586-orf-1 ID=SMED30007586-orf-1|Name=SMED30007586-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=116bp
MCCGIDNGADYKSTKWYNSPSNEFPKEGEVPLSCCTMEDEHFINTTACAH
SRPEDQSKYIYNKGCWDILRNVVSLTLYWSSIAVFILCGILLFVIIFSCI
MNRRINRDILYGQVV*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000418head
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR018499Tetraspanin/Peripherin
IPR008952Tetraspanin_EC2_sf
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 2..101
e-value: 2.3E-10
score: 40.6
NoneNo IPR availableTMHMMTMhelixcoord: 75..97