Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase

Overview


Name	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase
Smed ID	SMED30007572
Length (bp)	1300

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase (SMED30007572) t-SNE clustered cells

Violin plots show distribution of expression levels for Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase (SMED30007572) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase (SMED30007572) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30007572

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
parenchyma	SMED30007572	SMESG000008818.1	dd_Smed_v4_10223_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Human
Match: ALG3 (ALG3 alpha-1,3- mannosyltransferase [Source:HGNC Symbol;Acc:HGNC:23056])

HSP 1 Score: 320.472 bits (820), Expect = 8.044e-106
Identity = 181/343 (52.77%), Postives = 237/343 (69.10%), Query Frame = 1

Query:  184 KNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            ++TEIDW AYM EVEG INGT DY +LQG TGPLVYPAGFVY +  LY+ T +GT+I  AQ IF   Y+ TL L+F IY+Q   +P +VF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  ++ R+ WG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L  C  +Q+VLG+PFL+ N + YL+R+F+  RQFL+ WTVNWR +PE+ FL+R FHL LL  HL LLL+                +R+ S + +       N +   +F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST  SS 
Sbjct:   25 EDTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSA 367

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Human
Match: ALG3 (ALG3 alpha-1,3- mannosyltransferase [Source:HGNC Symbol;Acc:HGNC:23056])

HSP 1 Score: 320.087 bits (819), Expect = 9.019e-106
Identity = 181/342 (52.92%), Postives = 236/342 (69.01%), Query Frame = 1

Query:  187 NTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            +TEIDW AYM EVEG INGT DY +LQG TGPLVYPAGFVY +  LY+ T +GT+I  AQ IF   Y+ TL L+F IY+Q   +P +VF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  ++ R+ WG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L  C  +Q+VLG+PFL+ N + YL+R+F+  RQFL+ WTVNWR +PE+ FL+R FHL LL  HL LLL+                +R+ S + +       N +   +F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST  SS 
Sbjct:   18 DTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSA 359

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Human
Match: ALG3 (ALG3 alpha-1,3- mannosyltransferase [Source:HGNC Symbol;Acc:HGNC:23056])

HSP 1 Score: 319.316 bits (817), Expect = 8.261e-105
Identity = 181/341 (53.08%), Postives = 235/341 (68.91%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG INGT DY +LQG TGPLVYPAGFVY +  LY+ T +GT+I  AQ IF   Y+ TL L+F IY+Q   +P +VF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  ++ R+ WG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L  C  +Q+VLG+PFL+ N + YL+R+F+  RQFL+ WTVNWR +PE+ FL+R FHL LL  HL LLL+                +R+ S + +       N +   +F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST  SS 
Sbjct:   67 TEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSA 407

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Human
Match: ALG3 (ALG3 alpha-1,3- mannosyltransferase [Source:HGNC Symbol;Acc:HGNC:23056])

HSP 1 Score: 219.164 bits (557), Expect = 1.869e-67
Identity = 130/261 (49.81%), Postives = 176/261 (67.43%), Query Frame = 1

Query:  430 THIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
              +P +VF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  ++ R+ WG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L  C  +Q+VLG+PFL+ N + YL+R+F+  RQFL+ WTVNWR +PE+ FL+R FHL LL  HL LLL+                +R+ S + +       N +   +F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST  SS 
Sbjct:   51 AEVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSA 311

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Celegans
Match: algn-3 (pep chromosome:WBcel235:II:14127687:14132833:-1 gene:WBGene00010720.1 transcript:K09E4.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:algn-3)

HSP 1 Score: 278.1 bits (710), Expect = 5.490e-87
Identity = 152/349 (43.55%), Postives = 216/349 (61.89%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFIN-GTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRK--FMRNQSLKS---------ISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILW-SLPNISSI---------WRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW  YMQ+VE ++N G L+Y ++ G TGP VYPAG ++ +  L++ T  G NI  AQ IF GFY+  L ++FRI ++   IP  V + +  T YRIHSI++LRLFNDP+AMM  Y A++ F+  ++  G + +S+ VSIKMN+LLFAP L    L N  +  T+G+L+ CG +QL +G PFL+++   YL ++F+  R F++KWTVNWR +PE FFL++ FH  LL  HL  L V      F R   L++         I+ T            SN IGIA SRSLHYQFY WY+H +P +L+   P+++S+         W++ ++  +EL WN YPST +SS 
Sbjct:   45 TEIDWSTYMQQVECYVNKGILNYTQIGGDTGPCVYPAGHLFVFRLLHFFTSSGRNIRAAQHIFQGFYMFNLFMVFRILHKTMRIPPIVLLLVSITGYRIHSIFVLRLFNDPLAMMLFYVAMDRFLEQKWLVGCVFYSLAVSIKMNVLLFAPALFFTLLLNNSYMATLGYLTVCGLIQLYVGGPFLLYDWKSYLQQSFDLGRVFMFKWTVNWRFLPEEFFLDKRFHAALLVGHLTFLAVFASYMWFRRLNGLRTSVYLRLFEGITTTTGAVETYYAFCTSNLIGIAFSRSLHYQFYSWYFHQIPFLLFCDYPHVASVSTIPWRQFLWKVPLLAAVELSWNVYPSTWWSSA 393

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Celegans
Match: algn-3 (pep chromosome:WBcel235:II:14127687:14131752:-1 gene:WBGene00010720.1 transcript:K09E4.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:algn-3)

HSP 1 Score: 263.848 bits (673), Expect = 4.512e-82
Identity = 146/341 (42.82%), Postives = 210/341 (61.58%), Query Frame = 1

Query:  214 MQEVEGFIN-GTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRK--FMRNQSLKS---------ISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILW-SLPNISSI---------WRLLIMGLIELCWNTYPSTIYSSG 1170
            MQ+VE ++N G L+Y ++ G TGP VYPAG ++ +  L++ T  G NI  AQ IF GFY+  L ++FRI ++   IP  V + +  T YRIHSI++LRLFNDP+AMM  Y A++ F+  ++  G + +S+ VSIKMN+LLFAP L    L N  +  T+G+L+ CG +QL +G PFL+++   YL ++F+  R F++KWTVNWR +PE FFL++ FH  LL  HL  L V      F R   L++         I+ T            SN IGIA SRSLHYQFY WY+H +P +L+   P+++S+         W++ ++  +EL WN YPST +SS 
Sbjct:    1 MQQVECYVNKGILNYTQIGGDTGPCVYPAGHLFVFRLLHFFTSSGRNIRAAQHIFQGFYMFNLFMVFRILHKTMRIPPIVLLLVSITGYRIHSIFVLRLFNDPLAMMLFYVAMDRFLEQKWLVGCVFYSLAVSIKMNVLLFAPALFFTLLLNNSYMATLGYLTVCGLIQLYVGGPFLLYDWKSYLQQSFDLGRVFMFKWTVNWRFLPEEFFLDKRFHAALLVGHLTFLAVFASYMWFRRLNGLRTSVYLRLFEGITTTTGAVETYYAFCTSNLIGIAFSRSLHYQFYSWYFHQIPFLLFCDYPHVASVSTIPWRQFLWKVPLLAAVELSWNVYPSTWWSSA 341

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Fly
Match: Alg3 (gene:FBgn0011297 transcript:FBtr0072095)

HSP 1 Score: 238.81 bits (608), Expect = 3.856e-73
Identity = 125/232 (53.88%), Postives = 164/232 (70.69%), Query Frame = 1

Query:  163 IVLFYYTKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYF 858
            +V+      TEIDW+AYMQE EGF+NGT +Y  L+G TGPLVYPA FVY YSALY++T  GTN+  AQ+IF G Y++ L L+ R+Y++   +P YV V    TSYRIHSIY+LRLFNDP+A++ LYAA+N F+  R++ GS  FS+ V +KMNILLFAP LLL YL N G   T+  L+ CG +QL+LG PFL+ +  +YL  +F+  R F +KWTVN+R +    F NR F
Sbjct:   61 VVVIQRVPYTEIDWVAYMQECEGFLNGTTNYSLLRGDTGPLVYPAAFVYIYSALYYVTSHGTNVRLAQYIFAGIYLLQLALVLRLYSKSRKVPPYVLVLSAFTSYRIHSIYVLRLFNDPVAVLLLYAALNLFLDRRWTLGSTFFSLAVGVKMNILLFAPALLLFYLANLGLLRTILQLAVCGVIQLLLGAPFLLTHPVEYLRGSFDLGRIFEHKWTVNYRFLSRDLFENRTF 292

HSP 2 Score: 91.6633 bits (226), Expect = 1.356e-19
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1

Query:  961 TNLALV-MFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            T LAL+  F+ N +G+ACSRSLHYQFY+WY+H+LP + WS P    + R LI+GLIE CWNTYPST +SS 
Sbjct:  402 TQLALLPFFLCNLVGVACSRSLHYQFYVWYFHSLPYLAWSTPYSLGV-RCLILGLIEYCWNTYPSTNFSSA 471

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Zebrafish
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:ZFIN;Acc:ZDB-GENE-050522-334])

HSP 1 Score: 369.392 bits (947), Expect = 1.075e-124
Identity = 193/340 (56.76%), Postives = 249/340 (73.24%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXX-XXRKFMRNQS-----LKSIS---VTANKTN---LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS--LPNISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            TEIDW AYM EVEG INGT DY +L+G TGPLVYPAGFVY ++ LY+LTD G NI   Q++F  FY+I L L+ RIY++   +P YVF F+CC SYRIHSI+ILRLFNDP+AMM  + AIN F+  R++ G  L+S+ VS+KMN+LLFAPGLL + L   G W+T+  L+ C  +QLVLG+PFL+ N   Y++RAF+  RQFL+KWTVNWR +PE  FLNRYFHL LL  H+  LL+  ++++ R+ S     LK  S    TA+K N   + L++F SNFIG+  SRSLHYQFY+WY+HTLP +LWS  +  ++ + R+LI+GLIEL WNTYPST YSS
Sbjct:   61 TEIDWKAYMDEVEGVINGTYDYTQLKGDTGPLVYPAGFVYIFTGLYYLTDHGHNIRLGQYVFAVFYLINLLLVMRIYHRTKKVPPYVFFFICCASYRIHSIFILRLFNDPVAMMLCFGAINLFLDGRWTLGCALYSLAVSVKMNVLLFAPGLLFLLLCEFGLWKTLPRLALCAVIQLVLGLPFLLVNPVGYVSRAFDLGRQFLFKWTVNWRFLPEDVFLNRYFHLALLLAHITTLLLFALKRWKRSGSSIWTILKDPSERKETAHKVNADQMVLILFTSNFIGMCFSRSLHYQFYVWYFHTLPYLLWSGGVKKLAHLLRVLILGLIELSWNTYPSTNYSS 400

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Zebrafish
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:ZFIN;Acc:ZDB-GENE-050522-334])

HSP 1 Score: 118.242 bits (295), Expect = 2.426e-31
Identity = 72/132 (54.55%), Postives = 94/132 (71.21%), Query Frame = 1

Query:  718 LVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXX-XXRKFMRNQS-----LKSIS---VTANKTN---LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS 1077
            LVLG+PFL+ N   Y++RAF+  RQFL+KWTVNWR +PE  FLNRYFHL LL  H+  LL+  ++++ R+ S     LK  S    TA+K N   + L++F SNFIG+  SRSLHYQFY+WY+HTLP +LWS
Sbjct:    1 LVLGLPFLLVNPVGYVSRAFDLGRQFLFKWTVNWRFLPEDVFLNRYFHLALLLAHITTLLLFALKRWKRSGSSIWTILKDPSERKETAHKVNADQMVLILFTSNFIGMCFSRSLHYQFYVWYFHTLPYLLWS 132

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Xenopus
Match: gcn1 (GCN1, eIF2 alpha kinase activator homolog [Source:Xenbase;Acc:XB-GENE-5926092])

HSP 1 Score: 306.22 bits (783), Expect = 1.027e-99
Identity = 177/346 (51.16%), Postives = 235/346 (67.92%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXX--XXRKFMRNQSL------------KSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS-----LPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG +NGT DY +L+G+TGPLVYPAGFVY +S  Y+LT++G+NI  AQ+IF   Y++TL  +FRIY     +P YVF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  + + ++     +S+ VS+KMN+LL APGLL + L   G   ++  L  C  +Q++LG+PFL+ N   YL R+F+F RQFL++WTVNWR +PE  F +R FHL LL  HL  LL+  + R     QSL             S  +TANK  +  V+F SNF+G+  SRSLHYQFY+WY+H+LP +LW      LP ++ I   LI+GLIEL WNT+PST  SSG
Sbjct:   69 TEIDWKAYMDEVEGVLNGTYDYTQLKGETGPLVYPAGFVYIFSIFYYLTEQGSNIRLAQYIFAALYLLTLFFVFRIYVVTKKVPPYVFFFMCCASYRVHSIFLLRLFNDPVAMVILFFSINLLLQDHWNSACFFYSLAVSVKMNVLLLAPGLLYLLLCRFGLLHSIPKLCICAVLQVLLGLPFLLENPTGYLIRSFDFGRQFLFQWTVNWRFLPEFVFQHRAFHLALLTAHLATLLLFCVHRWPRSGQSLLALLKDPAQRKPSSHRLTANK--IIFVLFSSNFLGVMFSRSLHYQFYVWYFHSLPYLLWCTGTRILPGLAKI---LILGLIELSWNTFPSTALSSG 409

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Xenopus
Match: gcn1 (GCN1, eIF2 alpha kinase activator homolog [Source:Xenbase;Acc:XB-GENE-5926092])

HSP 1 Score: 304.679 bits (779), Expect = 1.242e-99
Identity = 177/346 (51.16%), Postives = 235/346 (67.92%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXX--XXRKFMRNQSL------------KSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS-----LPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG +NGT DY +L+G+TGPLVYPAGFVY +S  Y+LT++G+NI  AQ+IF   Y++TL  +FRIY     +P YVF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  + + ++     +S+ VS+KMN+LL APGLL + L   G   ++  L  C  +Q++LG+PFL+ N   YL R+F+F RQFL++WTVNWR +PE  F +R FHL LL  HL  LL+  + R     QSL             S  +TANK  +  V+F SNF+G+  SRSLHYQFY+WY+H+LP +LW      LP ++ I   LI+GLIEL WNT+PST  SSG
Sbjct:   24 TEIDWKAYMDEVEGVLNGTYDYTQLKGETGPLVYPAGFVYIFSIFYYLTEQGSNIRLAQYIFAALYLLTLFFVFRIYVVTKKVPPYVFFFMCCASYRVHSIFLLRLFNDPVAMVILFFSINLLLQDHWNSACFFYSLAVSVKMNVLLLAPGLLYLLLCRFGLLHSIPKLCICAVLQVLLGLPFLLENPTGYLIRSFDFGRQFLFQWTVNWRFLPEFVFQHRAFHLALLTAHLATLLLFCVHRWPRSGQSLLALLKDPAQRKPSSHRLTANK--IIFVLFSSNFLGVMFSRSLHYQFYVWYFHSLPYLLWCTGTRILPGLAKI---LILGLIELSWNTFPSTALSSG 364

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Xenopus
Match: gcn1 (GCN1, eIF2 alpha kinase activator homolog [Source:Xenbase;Acc:XB-GENE-5926092])

HSP 1 Score: 305.449 bits (781), Expect = 1.833e-99
Identity = 177/346 (51.16%), Postives = 235/346 (67.92%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXX--XXRKFMRNQSL------------KSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS-----LPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG +NGT DY +L+G+TGPLVYPAGFVY +S  Y+LT++G+NI  AQ+IF   Y++TL  +FRIY     +P YVF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  + + ++     +S+ VS+KMN+LL APGLL + L   G   ++  L  C  +Q++LG+PFL+ N   YL R+F+F RQFL++WTVNWR +PE  F +R FHL LL  HL  LL+  + R     QSL             S  +TANK  +  V+F SNF+G+  SRSLHYQFY+WY+H+LP +LW      LP ++ I   LI+GLIEL WNT+PST  SSG
Sbjct:   69 TEIDWKAYMDEVEGVLNGTYDYTQLKGETGPLVYPAGFVYIFSIFYYLTEQGSNIRLAQYIFAALYLLTLFFVFRIYVVTKKVPPYVFFFMCCASYRVHSIFLLRLFNDPVAMVILFFSINLLLQDHWNSACFFYSLAVSVKMNVLLLAPGLLYLLLCRFGLLHSIPKLCICAVLQVLLGLPFLLENPTGYLIRSFDFGRQFLFQWTVNWRFLPEFVFQHRAFHLALLTAHLATLLLFCVHRWPRSGQSLLALLKDPAQRKPSSHRLTANK--IIFVLFSSNFLGVMFSRSLHYQFYVWYFHSLPYLLWCTGTRILPGLAKI---LILGLIELSWNTFPSTALSSG 409

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Xenopus
Match: gcn1 (GCN1, eIF2 alpha kinase activator homolog [Source:Xenbase;Acc:XB-GENE-5926092])

HSP 1 Score: 305.449 bits (781), Expect = 2.047e-99
Identity = 177/346 (51.16%), Postives = 235/346 (67.92%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXX--XXRKFMRNQSL------------KSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS-----LPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG +NGT DY +L+G+TGPLVYPAGFVY +S  Y+LT++G+NI  AQ+IF   Y++TL  +FRIY     +P YVF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  + + ++     +S+ VS+KMN+LL APGLL + L   G   ++  L  C  +Q++LG+PFL+ N   YL R+F+F RQFL++WTVNWR +PE  F +R FHL LL  HL  LL+  + R     QSL             S  +TANK  +  V+F SNF+G+  SRSLHYQFY+WY+H+LP +LW      LP ++ I   LI+GLIEL WNT+PST  SSG
Sbjct:   69 TEIDWKAYMDEVEGVLNGTYDYTQLKGETGPLVYPAGFVYIFSIFYYLTEQGSNIRLAQYIFAALYLLTLFFVFRIYVVTKKVPPYVFFFMCCASYRVHSIFLLRLFNDPVAMVILFFSINLLLQDHWNSACFFYSLAVSVKMNVLLLAPGLLYLLLCRFGLLHSIPKLCICAVLQVLLGLPFLLENPTGYLIRSFDFGRQFLFQWTVNWRFLPEFVFQHRAFHLALLTAHLATLLLFCVHRWPRSGQSLLALLKDPAQRKPSSHRLTANK--IIFVLFSSNFLGVMFSRSLHYQFYVWYFHSLPYLLWCTGTRILPGLAKI---LILGLIELSWNTFPSTALSSG 409

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
Match: Alg3 (asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase) [Source:MGI Symbol;Acc:MGI:1098592])

HSP 1 Score: 321.242 bits (822), Expect = 1.050e-105
Identity = 182/341 (53.37%), Postives = 245/341 (71.85%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM +VEGFINGT DY +LQG TGPLVYPAGF+Y ++ L++ TD+GT+I  AQ IF   Y++TL L+F IY+Q + +P +VF FMCC SYR+HSI++LRLFNDP+AM  L+ +IN F++  +SWG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L+ C  +Q+VLG+PFL+ N   YL+R+F+  RQFL++WTVNWR +PE+ FL+R FHL LLA HL LLL+                +++ S + +   A   N +  ++F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST +SS 
Sbjct:   67 TEIDWKAYMAQVEGFINGTYDYTQLQGDTGPLVYPAGFLYIFTGLFYATDRGTDIPMAQNIFAVLYLVTLVLVFLIYHQTSKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMALLFLSINLFLAQCWSWGCCCFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLAICAALQVVLGLPFLLENPIGYLSRSFDLGRQFLFQWTVNWRFLPETIFLHRAFHLALLAAHLSLLLLFALCRWHRTGESILALLKDPSKRKVPPQALTPNQIVSILFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSFSSA 407

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
Match: Alg3 (asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase) [Source:MGI Symbol;Acc:MGI:1098592])

HSP 1 Score: 306.605 bits (784), Expect = 4.709e-101
Identity = 175/333 (52.55%), Postives = 238/333 (71.47%), Query Frame = 1

Query:  214 MQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            M +VEGFINGT DY +LQG TGPLVYPAGF+Y ++ L++ TD+GT+I  AQ IF   Y++TL L+F IY+Q + +P +VF FMCC SYR+HSI++LRLFNDP+AM  L+ +IN F++  +SWG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L+ C  +Q+VLG+PFL+ N   YL+R+F+  RQFL++WTVNWR +PE+ FL+R FHL LLA HL LLL+                +++ S + +   A   N +  ++F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST +SS 
Sbjct:    1 MAQVEGFINGTYDYTQLQGDTGPLVYPAGFLYIFTGLFYATDRGTDIPMAQNIFAVLYLVTLVLVFLIYHQTSKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMALLFLSINLFLAQCWSWGCCCFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLAICAALQVVLGLPFLLENPIGYLSRSFDLGRQFLFQWTVNWRFLPETIFLHRAFHLALLAAHLSLLLLFALCRWHRTGESILALLKDPSKRKVPPQALTPNQIVSILFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSFSSA 333

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
Match: Alg3 (asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase) [Source:MGI Symbol;Acc:MGI:1098592])

HSP 1 Score: 236.498 bits (602), Expect = 2.100e-74
Identity = 144/284 (50.70%), Postives = 198/284 (69.72%), Query Frame = 1

Query:  361 AQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            AQ IF   Y++TL L+F IY+Q + +P +VF FMCC SYR+HSI++LRLFNDP+AM  L+ +IN F++  +SWG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L+ C  +Q+VLG+PFL+ N   YL+R+F+  RQFL++WTVNWR +PE+ FL+R FHL LLA HL LLL+                +++ S + +   A   N +  ++F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST +SS 
Sbjct:    2 AQNIFAVLYLVTLVLVFLIYHQTSKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMALLFLSINLFLAQCWSWGCCCFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLAICAALQVVLGLPFLLENPIGYLSRSFDLGRQFLFQWTVNWRFLPETIFLHRAFHLALLAAHLSLLLLFALCRWHRTGESILALLKDPSKRKVPPQALTPNQIVSILFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSFSSA 285

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
Match: Alg3 (asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase) [Source:MGI Symbol;Acc:MGI:1098592])

HSP 1 Score: 203.371 bits (516), Expect = 3.172e-62
Identity = 100/176 (56.82%), Postives = 132/176 (75.00%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQ 717
            TEIDW AYM +VEGFINGT DY +LQG TGPLVYPAGF+Y ++ L++ TD+GT+I  AQ IF   Y++TL L+F IY+Q + +P +VF FMCC SYR+HSI++LRLFNDP+AM  L+ +IN F++  +SWG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L+ C  +Q
Sbjct:   67 TEIDWKAYMAQVEGFINGTYDYTQLQGDTGPLVYPAGFLYIFTGLFYATDRGTDIPMAQNIFAVLYLVTLVLVFLIYHQTSKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMALLFLSINLFLAQCWSWGCCCFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLAICAALQ 242

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
Match: Alg3 (asparagine-linked glycosylation 3 (alpha-1,3-mannosyltransferase) [Source:MGI Symbol;Acc:MGI:1098592])

HSP 1 Score: 179.489 bits (454), Expect = 2.506e-53
Identity = 88/164 (53.66%), Postives = 122/164 (74.39%), Query Frame = 1

Query:  361 AQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNR 852
            AQ IF   Y++TL L+F IY+Q + +P +VF FMCC SYR+HSI++LRLFNDP+AM  L+ +IN F++  +SWG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L+ C  +Q+VLG+PFL+ N   YL+R+F+  RQFL++WTVNWR +PE+ FL+R
Sbjct:    2 AQNIFAVLYLVTLVLVFLIYHQTSKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMALLFLSINLFLAQCWSWGCCCFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLAICAALQVVLGLPFLLENPIGYLSRSFDLGRQFLFQWTVNWRFLPETIFLHR 165

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
Match: sp|Q8K2A8|ALG3_MOUSE (Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg3 PE=1 SV=2)

HSP 1 Score: 321.242 bits (822), Expect = 7.341e-105
Identity = 182/341 (53.37%), Postives = 245/341 (71.85%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM +VEGFINGT DY +LQG TGPLVYPAGF+Y ++ L++ TD+GT+I  AQ IF   Y++TL L+F IY+Q + +P +VF FMCC SYR+HSI++LRLFNDP+AM  L+ +IN F++  +SWG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L+ C  +Q+VLG+PFL+ N   YL+R+F+  RQFL++WTVNWR +PE+ FL+R FHL LLA HL LLL+                +++ S + +   A   N +  ++F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST +SS 
Sbjct:   67 TEIDWKAYMAQVEGFINGTYDYTQLQGDTGPLVYPAGFLYIFTGLFYATDRGTDIPMAQNIFAVLYLVTLVLVFLIYHQTSKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMALLFLSINLFLAQCWSWGCCCFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLAICAALQVVLGLPFLLENPIGYLSRSFDLGRQFLFQWTVNWRFLPETIFLHRAFHLALLAAHLSLLLLFALCRWHRTGESILALLKDPSKRKVPPQALTPNQIVSILFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSFSSA 407

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
Match: sp|Q92685|ALG3_HUMAN (Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Homo sapiens OX=9606 GN=ALG3 PE=1 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 3.967e-104
Identity = 181/341 (53.08%), Postives = 235/341 (68.91%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXR-----------KFMRNQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPN--ISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG INGT DY +LQG TGPLVYPAGFVY +  LY+ T +GT+I  AQ IF   Y+ TL L+F IY+Q   +P +VF FMCC SYR+HSI++LRLFNDP+AM+ L+ +IN  ++ R+ WG   FS+ VS+KMN+LLFAPGLL + L   GF   +  L  C  +Q+VLG+PFL+ N + YL+R+F+  RQFL+ WTVNWR +PE+ FL+R FHL LL  HL LLL+                +R+ S + +       N +   +F SNFIGI  SRSLHYQFY+WY+HTLP +LW++P   ++ + RLL++GLIEL WNTYPST  SS 
Sbjct:   67 TEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSA 407

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
Match: sp|Q55F69|ALG3_DICDI (Probable Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg3 PE=3 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 1.701e-89
Identity = 162/375 (43.20%), Postives = 225/375 (60.00%), Query Frame = 1

Query:  166 VLFYYTKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQ------------------------------FTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQS------LKSISVTANKTNLA-----LVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            ++  Y K TEIDW  Y+Q+VE F+ G  DY K++G TGP VYPAG +Y +S LY LT+ G NIL AQ+IF   Y++   ++F IY +                              F  IP Y+ +F+C  S RIHSI+ LR+FND I+M+  Y ++   +  R++ G + FS  VS+KMN+LL++P LL + L   G W T+  L  CG VQ++L +PFL+ N   YL RAFEF RQFLYKWTVNWR +PE  FLN+ + L LL++HL+ +++   K+++ +       LK  S+  N   L+     L+MF  N IG+  SRSLHYQFY+WY+HTLP +LWS  N +   ++  M + E  WNTYPST  SS
Sbjct:   35 IIIDYVKYTEIDWSTYVQQVEVFLKGIYDYTKIEGDTGPCVYPAGHLYVFSLLYNLTENGLNILKAQYIFAAIYILLTFIVFSIYGESIKESIKQITNKSNNNNNNNNNNNNNNNNVFLKIPFYIIIFLCL-SKRIHSIFALRMFNDCISMLLFYISLYLIIKGRWNLGCLFFSCSVSVKMNVLLYSPALLFLLLSTFGIWRTIPKLMICGLVQVLLAIPFLMVNPQGYLLRAFEFSRQFLYKWTVNWRFLPEHLFLNKSWALFLLSIHLLFIILFFIKYIKKEQGGISNILKRGSINQNPKLLSVNYILLIMFTINLIGVTFSRSLHYQFYLWYFHTLPFLLWS-TNYNLFLKIGFMCITEYAWNTYPSTSTSS 407

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
Match: sp|P82149|NT53_DROME (Lethal(2)neighbour of tid protein 2 OS=Drosophila melanogaster OX=7227 GN=l(2)not2 PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.569e-72
Identity = 125/232 (53.88%), Postives = 164/232 (70.69%), Query Frame = 1

Query:  163 IVLFYYTKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYF 858
            +V+      TEIDW+AYMQE EGF+NGT +Y  L+G TGPLVYPA FVY YSALY++T  GTN+  AQ+IF G Y++ L L+ R+Y++   +P YV V    TSYRIHSIY+LRLFNDP+A++ LYAA+N F+  R++ GS  FS+ V +KMNILLFAP LLL YL N G   T+  L+ CG +QL+LG PFL+ +  +YL  +F+  R F +KWTVN+R +    F NR F
Sbjct:   61 VVVIQRVPYTEIDWVAYMQECEGFLNGTTNYSLLRGDTGPLVYPAAFVYIYSALYYVTSHGTNVRLAQYIFAGIYLLQLALVLRLYSKSRKVPPYVLVLSAFTSYRIHSIYVLRLFNDPVAVLLLYAALNLFLDRRWTLGSTFFSLAVGVKMNILLFAPALLLFYLANLGLLRTILQLAVCGVIQLLLGAPFLLTHPVEYLRGSFDLGRIFEHKWTVNYRFLSRDVFENRTF 292

HSP 2 Score: 91.6633 bits (226), Expect = 1.299e-18
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1

Query:  961 TNLALV-MFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            T LAL+  F+ N +G+ACSRSLHYQFY+WY+H+LP + WS P    + R LI+GLIE CWNTYPST +SS 
Sbjct:  402 TQLALLPFFLCNLVGVACSRSLHYQFYVWYFHSLPYLAWSTPYSLGV-RCLILGLIEYCWNTYPSTNFSSA 471

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
Match: sp|Q27333|NT56_DROME (Lethal(2)neighbour of tid protein OS=Drosophila melanogaster OX=7227 GN=l(2)not PE=1 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 4.119e-72
Identity = 125/232 (53.88%), Postives = 164/232 (70.69%), Query Frame = 1

Query:  163 IVLFYYTKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYF 858
            +V+      TEIDW+AYMQE EGF+NGT +Y  L+G TGPLVYPA FVY YSALY++T  GTN+  AQ+IF G Y++ L L+ R+Y++   +P YV V    TSYRIHSIY+LRLFNDP+A++ LYAA+N F+  R++ GS  FS+ V +KMNILLFAP LLL YL N G   T+  L+ CG +QL+LG PFL+ +  +YL  +F+  R F +KWTVN+R +    F NR F
Sbjct:   61 VVVIQRVPYTEIDWVAYMQECEGFLNGTTNYSLLRGDTGPLVYPAAFVYIYSALYYVTSHGTNVRLAQYIFAGIYLLQLALVLRLYSKSRKVPPYVLVLSAFTSYRIHSIYVLRLFNDPVAVLLLYAALNLFLDRRWTLGSTFFSLAVGVKMNILLFAPALLLFYLANLGLLRTILQLAVCGVIQLLLGAPFLLTHPVEYLRGSFDLGRIFEHKWTVNYRFLSRDVFENRTF 292

HSP 2 Score: 91.6633 bits (226), Expect = 1.334e-18
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1

Query:  961 TNLALV-MFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            T LAL+  F+ N +G+ACSRSLHYQFY+WY+H+LP + WS P    + R LI+GLIE CWNTYPST +SS 
Sbjct:  402 TQLALLPFFLCNLVGVACSRSLHYQFYVWYFHSLPYLAWSTPYSLGV-RCLILGLIEYCWNTYPSTNFSSA 471

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
Match: A0A419QE81 (Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1 OS=Clonorchis sinensis OX=79923 GN=ALG3 PE=4 SV=1)

HSP 1 Score: 385.956 bits (990), Expect = 3.088e-128
Identity = 198/339 (58.41%), Postives = 248/339 (73.16%), Query Frame = 1

Query:  184 KNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRK----------FMRNQSLKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            ++TEIDWIAYMQEVEGF+NGTLDY+KL+GQTGP VYPAGF+Y Y  LY +T +GT I  AQ+IF+GFY++TL L+F IY     +P YVFVFMC  SYRIHSIY+LRLFNDP+AM+ LY  +N  +  +++   I FS+GVSIKMNILLFAPGL LI L+  G  ET GHL EC  VQLVLG PFL HN   Y++RAF F RQF+YKWTVNWR+IPE  FL+R FH +LL +H   L++L+ K          F++ Q         + + +   MF+SNFIG+  SRSLHYQFY+WY+HT+P +LW+    SS  +LLI GLIE+CWNTYPST++SSG
Sbjct:   14 QDTEIDWIAYMQEVEGFVNGTLDYDKLEGQTGPCVYPAGFLYIYWMLYQITSRGTLIYLAQYIFIGFYLLTLVLVFNIYRLSCQVPPYVFVFMCVISYRIHSIYVLRLFNDPLAMIALYTCVNFLLYGKFTLACIFFSLGVSIKMNILLFAPGLFLILLYYRGILETAGHLCECIIVQLVLGAPFLFHNYEAYISRAFNFGRQFMYKWTVNWRIIPEDVFLDRRFHAVLLGLHTAFLILLLFKWIRYRGGFSEFLKLQIPPDRDPRKDISGIIYPMFLSNFIGVTFSRSLHYQFYVWYFHTIPYLLWTTCLPSSPLKLLIFGLIEVCWNTYPSTVFSSG 352

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
Match: A0A4V3SEY5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005350 PE=4 SV=1)

HSP 1 Score: 383.259 bits (983), Expect = 9.607e-127
Identity = 196/340 (57.65%), Postives = 247/340 (72.65%), Query Frame = 1

Query:  181 TKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXX----------RKFMRNQSLKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
             K TEIDWIAYMQEVEGF+NGTLDY+KL+GQTGP VYPAGF+Y Y  LY +T +GT I  AQ+IF+G Y++TL L+F IY     +P YVFVFMC  SYRIHSIY+LRLFNDP+AM+ LYA +N  +  +++   I FS+GVS+KMNILLFAPGL LI L+  G  ET GHL EC  VQLVLG PFL HN   Y++RAF F RQF+YKWTVNWR+IPE  FL+R FH +LL +H   L++L+           +F++ Q         + + +   MF+SNFIG+  SRSLHYQFY+WY+HT+P +LW+    S+  +LLI GLIE+CWNTYPST++SSG
Sbjct:   42 VKYTEIDWIAYMQEVEGFVNGTLDYDKLEGQTGPCVYPAGFLYIYWMLYQITSRGTLIHLAQYIFIGLYLLTLVLVFNIYRLSCQVPPYVFVFMCVISYRIHSIYVLRLFNDPLAMIALYACVNFLLYGKFTLACIFFSLGVSVKMNILLFAPGLFLILLYYRGILETAGHLCECIIVQLVLGAPFLFHNYEAYISRAFNFGRQFMYKWTVNWRIIPEDVFLDRRFHAVLLGLHAAFLIILLFRWIRYRGGFSQFLKLQIPPDRDPRKDMSGVIYPMFLSNFIGVTFSRSLHYQFYVWYFHTIPYLLWTTSLPSNPLKLLIFGLIEVCWNTYPSTVFSSG 381

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
Match: A0A504YLI2 (Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase OS=Fasciola gigantica OX=46835 GN=FGIG_02728 PE=4 SV=1)

HSP 1 Score: 379.022 bits (972), Expect = 3.826e-125
Identity = 190/339 (56.05%), Postives = 250/339 (73.75%), Query Frame = 1

Query:  181 TKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQ-------SLKSISVTANKTNLALV--MFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
             K TEIDW+AYMQEVE F+NGTLDY++++GQTGP VYPAGF+Y YS LY +T  G NIL +Q+IF+ FY++TL L+F IY   + +P Y  VFMC  SYRIHSIYILRLFNDP+AM+ +YA+IN  +  +++  SIL S+GVSIKMN+LLF PG LL+ L + G  +TVGHL ECG VQLVLG PFL HN   Y+  AF F RQF+Y+WTVNWR+IPE  FL+R FH++L+ +HL  + + + +F+R++       SL     T      +++  +F+ NF+G+A SRSLHYQFY+WY+HTL  +LWS   + +  R L++GLIELCWNTYPST+YSSG
Sbjct:   42 VKYTEIDWVAYMQEVEHFLNGTLDYDQIKGQTGPCVYPAGFLYIYSLLYKITGGGANILLSQYIFMFFYILTLLLVFNIYRISSRVPPYALVFMCIISYRIHSIYILRLFNDPLAMIAVYASINALLYGQFTLASILMSLGVSIKMNVLLFFPGFLLVILLHRGILQTVGHLCECGIVQLVLGAPFLFHNPESYVTSAFNFGRQFMYQWTVNWRIIPEYLFLDRRFHMMLMVLHLFFVALTLFRFIRSRGGLTRFLSLHKPKCTTRPDAASVLYPLFVCNFVGVAFSRSLHYQFYVWYFHTLLYLLWSNGQLPTPVRFLLLGLIELCWNTYPSTVYSSG 380

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
Match: A0A074ZDK6 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_08463 PE=4 SV=1)

HSP 1 Score: 377.481 bits (968), Expect = 3.459e-124
Identity = 196/344 (56.98%), Postives = 247/344 (71.80%), Query Frame = 1

Query:  184 KNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVY-----PAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRK----------FMRNQSLKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            ++TEIDWIAYMQEVEGF+NGTLDY+KL+GQTGP VY     PAGF+Y Y  LY +T +GT I  AQ+IF+G Y++TL L+F IY     +P YVFVFMC  SYRIHSIY+LRLFNDP+AM+ LYA +N  +  +++   I FS+GVS+KMNILLFAPGL LI L+  G  ET GHL EC  VQLV+G PFL HN   Y++RAF F RQF+YKWTVNWR+IPE  FL+R FH +LL +H   L++L+ K          F+  Q         + + +   MF+SNFIG+  SRSLHYQFY+WY+HT+P +LW+    SS  +LLI GLIE+CWNTYPST++SSG
Sbjct:   58 QDTEIDWIAYMQEVEGFVNGTLDYDKLEGQTGPCVYVRRLYPAGFLYIYWMLYQITSRGTLIHLAQYIFIGLYLLTLVLVFNIYRLSCQVPPYVFVFMCVISYRIHSIYVLRLFNDPLAMIALYACVNFLLYGKFTLACIFFSLGVSVKMNILLFAPGLFLILLYYRGILETAGHLCECIIVQLVVGAPFLFHNYEAYISRAFNFGRQFMYKWTVNWRIIPEDVFLDRRFHAVLLGLHATFLIILLFKWVRYRGGFSEFLELQVPPDRDPRKDMSGVIYPMFISNFIGVTFSRSLHYQFYVWYFHTIPYLLWTTSLPSSPLKLLIFGLIEVCWNTYPSTVFSSG 401

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
Match: A0A4Z2CND4 (Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase OS=Schistosoma japonicum OX=6182 GN=EWB00_009220 PE=4 SV=1)

HSP 1 Score: 376.711 bits (966), Expect = 3.869e-124
Identity = 200/336 (59.52%), Postives = 250/336 (74.40%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQS-----LKSISVTAN-KTNLALV---MFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDWIAYMQ+V GF+NGTLDY+KL+GQTGP VYPAG +Y Y+ L+W+TD G+ I  AQF+FLG Y+ITL L+F IY      P Y   FMC  SYRIHSIY+LRLFNDP+AM FLYA+IN F+ NR++  SILFS+GVS+KMNILLF P  L++ +++ G  ET+GHL EC  VQL +G PFL HNS  YL+ AF F RQF+YKWTVNWR +PES FL+R FHL+LL  HL LLL+   KF+R++      L       N K + A V   MF+ NF+G+  SRSLHYQFYIWY+HT+P +LW + + S+  +LLI+GLIE+ WNTYPSTI+SSG
Sbjct:   45 TEIDWIAYMQQVSGFLNGTLDYDKLEGQTGPCVYPAGHLYVYTFLHWITDGGSVIKTAQFVFLGLYIITLMLVFNIYRLSFQAPPYSLFFMCIVSYRIHSIYLLRLFNDPVAMFFLYASINAFLYNRFTVASILFSLGVSVKMNILLFLPAFLIVLVWHKGLLETIGHLCECFVVQLAVGTPFLFHNSWAYLSSAFNFSRQFMYKWTVNWRFLPESIFLDRQFHLILLIFHLCLLLIFFVKFIRSRGGLWNYLCIFDPERNPKFDPAAVLYPMFVCNFVGVVVSRSLHYQFYIWYFHTIPYLLWCVKSFSTPVKLLILGLIEISWNTYPSTIWSSG 380

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Cavefish
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:NCBI gene;Acc:103037087])

HSP 1 Score: 343.199 bits (879), Expect = 1.052e-114
Identity = 184/340 (54.12%), Postives = 242/340 (71.18%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXX-XXXRKFMRNQS-----------LKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS--LPNISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            TEIDW AYM EVEG INGT DY +L+G TGPLVYPAGFVY ++ALY++TD+G NI   Q++F  FY+ITL L+FRIY++   +P YVF F+CC SYRIHSI++LRLFNDP+AMM L+ A+N F+   ++ G  L+S+ VS+KMN++LFAPGLL + L   G  +T+  L  C  +QL+LG+PFL+ N   Y++RAF+  RQFL+KWTVNWR +PE  FL+RYFHL LL  HL  L+   +R++ R  S            K  +   N   +  V+F SNFIG+   RSLHYQFY+WY+HTLP +LWS  +  ++ + R+LI+GLIEL WNTYPST YSS
Sbjct:   61 TEIDWKAYMDEVEGVINGTYDYTELKGDTGPLVYPAGFVYIFTALYYITDRGLNIRLGQYVFAVFYLITLLLVFRIYHRTKKVPPYVFFFVCCASYRIHSIFVLRLFNDPVAMMLLFGAVNLFLDAHWTLGCALYSLAVSVKMNVMLFAPGLLFLLLSEFGLIKTLPRLMLCAVIQLLLGLPFLLVNPVGYMSRAFDLGRQFLFKWTVNWRFLPEEIFLSRYFHLALLLAHLTTLVFFALRRWKRPGSSIWALLKEPSERKETAQKLNSDQIITVLFTSNFIGMCFCRSLHYQFYVWYFHTLPYLLWSGGVKKLAHLLRVLILGLIELSWNTYPSTNYSS 400

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Cavefish
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:NCBI gene;Acc:103037087])

HSP 1 Score: 342.043 bits (876), Expect = 4.113e-114
Identity = 184/340 (54.12%), Postives = 242/340 (71.18%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXX-XXXRKFMRNQS-----------LKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS--LPNISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            TEIDW AYM EVEG INGT DY +L+G TGPLVYPAGFVY ++ALY++TD+G NI   Q++F  FY+ITL L+FRIY++   +P YVF F+CC SYRIHSI++LRLFNDP+AMM L+ A+N F+   ++ G  L+S+ VS+KMN++LFAPGLL + L   G  +T+  L  C  +QL+LG+PFL+ N   Y++RAF+  RQFL+KWTVNWR +PE  FL+RYFHL LL  HL  L+   +R++ R  S            K  +   N   +  V+F SNFIG+   RSLHYQFY+WY+HTLP +LWS  +  ++ + R+LI+GLIEL WNTYPST YSS
Sbjct:   61 TEIDWKAYMDEVEGVINGTYDYTELKGDTGPLVYPAGFVYIFTALYYITDRGLNIRLGQYVFAVFYLITLLLVFRIYHRTKKVPPYVFFFVCCASYRIHSIFVLRLFNDPVAMMLLFGAVNLFLDAHWTLGCALYSLAVSVKMNVMLFAPGLLFLLLSEFGLIKTLPRLMLCAVIQLLLGLPFLLVNPVGYMSRAFDLGRQFLFKWTVNWRFLPEEIFLSRYFHLALLLAHLTTLVFFALRRWKRPGSSIWALLKEPSERKETAQKLNSDQIITVLFTSNFIGMCFCRSLHYQFYVWYFHTLPYLLWSGGVKKLAHLLRVLILGLIELSWNTYPSTNYSS 400

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Cavefish
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:NCBI gene;Acc:103037087])

HSP 1 Score: 339.347 bits (869), Expect = 3.666e-113
Identity = 181/330 (54.85%), Postives = 241/330 (73.03%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQSLKSISVTANK--TNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS--LPNISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            TEIDW AYM EVEG INGT DY +L+G TGPLVYPAGFVY ++ALY++TD+G NI   Q++F  FY+ITL L+FRIY++   +P YVF F+CC SYRIHSI++LRLFNDP+AMM L+ A+N F+   ++ G  L+S+ VS+KMN++LFAPGLL + L   G  +T+  L  C  +QL+LG+PFL+ N   Y++RAF+  RQFL+KWTVNWR +PE  FL+RYFHL LL  HL  L+    +  ++ +L  + V      + +  V+F SNFIG+   RSLHYQFY+WY+HTLP +LWS  +  ++ + R+LI+GLIEL WNTYPST YSS
Sbjct:   61 TEIDWKAYMDEVEGVINGTYDYTELKGDTGPLVYPAGFVYIFTALYYITDRGLNIRLGQYVFAVFYLITLLLVFRIYHRTKKVPPYVFFFVCCASYRIHSIFVLRLFNDPVAMMLLFGAVNLFLDAHWTLGCALYSLAVSVKMNVMLFAPGLLFLLLSEFGLIKTLPRLMLCAVIQLLLGLPFLLVNPVGYMSRAFDLGRQFLFKWTVNWRFLPEEIFLSRYFHLALLLAHLTTLVFFALRRWKSHALFLLCVFLFNWLSQIITVLFTSNFIGMCFCRSLHYQFYVWYFHTLPYLLWSGGVKKLAHLLRVLILGLIELSWNTYPSTNYSS 390

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Sea Lamprey
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:ZFIN;Acc:ZDB-GENE-050522-334])

HSP 1 Score: 286.189 bits (731), Expect = 4.593e-94
Identity = 156/319 (48.90%), Postives = 212/319 (66.46%), Query Frame = 1

Query:  259 KLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQS------LKSISVTANKTN------LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSI----WRLLIMGLIELCWNTYPSTIYSS 1167
            KLQG TGPLVYPAGFVY YS LY +T++G ++  AQ+IF   Y++TL L+FRIY +   +P Y   FMCC SYR+HSI++LRLFNDP+AM  L+A++N  +  R++ G ++FS+ VS+KMN+LLFAPGLL + L   G    V  L+ C  +Q+VLG+PFL+ N   Y  RAF+  R FL++WTVNWRL+PE  FL+R FH  LLA HL +LL+   +            LK+ +    +        +A V+F SN +G+  +RSLHYQFY+WY+H+LP +LW  P  S       R+L++G+IEL WNTYPST  SS
Sbjct:    2 KLQGGTGPLVYPAGFVYVYSLLYHVTERGADVRRAQYIFAVLYLVTLVLVFRIYARVRRVPPYALFFMCCASYRVHSIFVLRLFNDPVAMAILFASVNLLLDRRWTLGCLVFSLAVSVKMNVLLFAPGLLFVLLRELGPARAVPRLAACAALQVVLGLPFLLANPLGYATRAFDLGRSFLFEWTVNWRLLPEWLFLHRGFHAALLAAHLAVLLLYAHRHWSRPGEGLLGLLKAPTKKKKRDPMTDDHIMAFVLFSSNLVGVCFARSLHYQFYVWYFHSLPFLLWCCPLGSMAPLLRHRVLVLGVIELAWNTYPSTKISS 320

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Yeast
Match: ALG3 (Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant [Source:SGD;Acc:S000000178])

HSP 1 Score: 179.874 bits (455), Expect = 3.885e-52
Identity = 134/356 (37.64%), Postives = 193/356 (54.21%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGF-INGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAI-------NCFMSNRYSWG-----SILFSIGVSIKMNILLFAPGLL--LIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXX-XRKFMR----------NQSLKSISVTANKTN-LALVMFMSNFIGIACSRSLHYQFYIWYYHTLP-LILWS-LP-NISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            TEID+ AYM+++E   ++G LDY ++ G TGPLVYPAG V  Y  +YWLT+   ++   Q  F   Y++TL L    Y    H+P +  V + C S R+HSIY+LRLFND    +F+   +        C    +         S  +S+ VSIKMN LL+ P ++  L  L +     T+  L      Q+ + +PFL     QYL+ AF F R+F+Y+W++NW+++ E  F ++ FHL LL  HL+ L  L V ++ R          +   K+  + AN    +  V+  SNFIG+  SRSLHYQF  WY+ TLP LI WS +P  +  IW +    L E CWN+YP    +S
Sbjct:   68 TEIDYKAYMEQIEMIQLDGMLDYSQVSGGTGPLVYPAGHVLIYKMMYWLTEGMDHVERGQVFFRYLYLLTLALQMACY-YLLHLPPWCVV-LACLSKRLHSIYVLRLFNDCFTTLFMVVTVLGAIVASRCHQRPKLKKSLALVISATYSMAVSIKMNALLYFPAMMISLFILNDANVILTLLDLVAMIAWQVAVAVPFLRSFPQQYLHCAFNFGRKFMYQWSINWQMMDEEAFNDKRFHLALLISHLIALTTLFVTRYPRILPDLWSSLCHPLRKNAVLNANPAKTIPFVLIASNFIGVLFSRSLHYQFLSWYHWTLPILIFWSGMPFFVGPIWYV----LHEWCWNSYPPNSQAS 417

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Nematostella
Match: EDO46580 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPJ4])

HSP 1 Score: 355.14 bits (910), Expect = 1.803e-120
Identity = 190/340 (55.88%), Postives = 246/340 (72.35%), Query Frame = 1

Query:  163 IVLFYYTKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQ---SLKSISVTANKTN--LALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSS 1167
            I + +  K TEIDW AYM EVEGF+NGT DY KLQG TGPLVYPAGFVY +S LY LT KGTNI  AQ++F   Y++TL  +F IYN+   +P YVF FMCC SYRIHSI+ILRLFNDP+AM+FLY ++N F++N ++ G +LFS GVS+KMN+LLFAPGLL + L   G W T+  L+ CG  Q++LG+PFL+ N   Y++R+F   RQF + WTVNWR +PE  FLNRYFH  LL +HL+ L        R +   +L+S+  +   +N  L  ++F SNF+G+  SRSLHYQFY+WY+HT+P +LW+    +S  R+LI+G+IELCWNTYPSTI SS
Sbjct:   24 IAVIWKIKYTEIDWEAYMSEVEGFLNGTYDYTKLQGATGPLVYPAGFVYIFSGLYHLTSKGTNIRMAQYLFAALYLLTLISVFSIYNKTKKVPPYVFFFMCCASYRIHSIFILRLFNDPVAMIFLYCSVNAFLANNWNLGCLLFSFGVSVKMNVLLFAPGLLFLLLKKFGAWRTIPKLAICGVPQVLLGLPFLMDNPVGYISRSFNLGRQFFFIWTVNWRFLPEWLFLNRYFHAALLLLHLLTLFCFYLYKWRGKRLYALRSLKKSGAHSNDLLVTILFTSNFLGMVFSRSLHYQFYVWYFHTIPYLLWTTRLWTSA-RILILGVIELCWNTYPSTIASS 362

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Medaka
Match: alg3 (ALG3 alpha-1,3- mannosyltransferase [Source:NCBI gene;Acc:101167399])

HSP 1 Score: 342.428 bits (877), Expect = 4.976e-114
Identity = 194/335 (57.91%), Postives = 245/335 (73.13%), Query Frame = 1

Query:  190 TEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXX--XRKFMRNQS----LKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWS--LPNISSIWRLLIMGLIELCWNTYPSTIYSSG 1170
            TEIDW AYM EVEG INGT DY +L+G TGPLVYPAGFVY ++ALY++T+ GTNI   Q+IF  FY+ITL L+FRIYN+   +P YVF F+CC SYRIHSI++LRLFNDP+AMM L+A+IN F+   ++ G  L+S+ VS+KMN+LLFAPGLL + L+  G   T+ +LS C  +QL+LG+PFL+ N   Y++RAF+  RQFL+KWTVNWR +PE  FLNRYFHL LLA HL+ LL+    R     QS    LK  S         LV+F SNFIG+  SRSLHYQFY+WY+HTLP +LWS  +  ++ + R+LI+GLIEL WNTYPST  SS 
Sbjct:   80 TEIDWKAYMDEVEGVINGTYDYTQLKGDTGPLVYPAGFVYVFTALYYITNHGTNIRLGQYIFAVFYLITLLLVFRIYNRTKKVPPYVFFFVCCASYRIHSIFVLRLFNDPVAMMLLFASINLFIDGYWTVGCGLYSLAVSVKMNVLLFAPGLLFLLLWEFGLMRTIPNLSLCAGIQLLLGLPFLLENPVGYVSRAFDLGRQFLFKWTVNWRFLPEWLFLNRYFHLALLAAHLLALLLFALTRWKRPGQSVFELLKEPSKRKVPAQKILVLFTSNFIGMCFSRSLHYQFYVWYFHTLPYLLWSGGVEKLAHLLRVLILGLIELSWNTYPSTNSSSA 414

[Close]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Planmine SMEST
Match: SMESG000008818.1 (SMESG000008818.1)

HSP 1 Score: 668.692 bits (1724), Expect = 0.000e+0
Identity = 362/366 (98.91%), Postives = 364/366 (99.45%), Query Frame = 1

Query:  181 TKNTEIDWIAYMQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFXXXXXXXXXXXXXXXXRKFMRNQSLKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSGXXXXXXXXXXXXXXKGNGFNYITSMVAISETKQKLK 1278
             K TEIDWIAYMQEVEGF+NGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFHLLLLAVHLVLLLVLVRKFMRNQSLKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFY+WYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSGLLHLCHLFVLFHWLKGNGFNYITSMVAISETKQKLK
Sbjct:   40 VKYTEIDWIAYMQEVEGFLNGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFAQFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFNDPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLFNCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTVNWRLIPESFFLNRYFHLLLLAVHLVLLLVLVRKFMRNQSLKSISVTANKTNLALVMFMSNFIGIACSRSLHYQFYVWYYHTLPLILWSLPNISSIWRLLIMGLIELCWNTYPSTIYSSGLLHLCHLFVLFHWLKGNGFNYITSMVAISETKQKLK 405

HSP 2 Score: 55.0694 bits (131), Expect = 5.574e-8
Identity = 39/41 (95.12%), Postives = 41/41 (100.00%), Query Frame = 2

Query:   71 MSSFCEKLEWIFYNKSWKRTXXXXXXXXXXXXSYFIILKIQ 193
            MSSFCEKLEWIFYNKSWKRTLMLLLLFLEAGLSYFIILK++
Sbjct:    1 MSSFCEKLEWIFYNKSWKRTLMLLLLFLEAGLSYFIILKVK 41

The following BLAST results are available for this feature:

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Human
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Celegans
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Fly
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Zebrafish
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Xenopus
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Cavefish
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Sea Lamprey
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Yeast
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Nematostella
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Medaka
BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Planmine SMEST

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
ALG3	8.044e-106	52.77	ALG3 alpha-1,3- mannosyltransferase [Source:HGNC S...	[more]
ALG3	9.019e-106	52.92	ALG3 alpha-1,3- mannosyltransferase [Source:HGNC S...	[more]
ALG3	8.261e-105	53.08	ALG3 alpha-1,3- mannosyltransferase [Source:HGNC S...	[more]
ALG3	1.869e-67	49.81	ALG3 alpha-1,3- mannosyltransferase [Source:HGNC S...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
algn-3	5.490e-87	43.55	pep chromosome:WBcel235:II:14127687:14132833:-1 ge...	[more]
algn-3	4.512e-82	42.82	pep chromosome:WBcel235:II:14127687:14131752:-1 ge...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Alg3	3.856e-73	53.88	gene:FBgn0011297 transcript:FBtr0072095	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
alg3	1.075e-124	56.76	ALG3 alpha-1,3- mannosyltransferase [Source:ZFIN;A...	[more]
alg3	2.426e-31	54.55	ALG3 alpha-1,3- mannosyltransferase [Source:ZFIN;A...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
gcn1	1.027e-99	51.16	GCN1, eIF2 alpha kinase activator homolog [Source:...	[more]
gcn1	1.242e-99	51.16	GCN1, eIF2 alpha kinase activator homolog [Source:...	[more]
gcn1	1.833e-99	51.16	GCN1, eIF2 alpha kinase activator homolog [Source:...	[more]
gcn1	2.047e-99	51.16	GCN1, eIF2 alpha kinase activator homolog [Source:...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Alg3	1.050e-105	53.37	asparagine-linked glycosylation 3 (alpha-1,3-manno...	[more]
Alg3	4.709e-101	52.55	asparagine-linked glycosylation 3 (alpha-1,3-manno...	[more]
Alg3	2.100e-74	50.70	asparagine-linked glycosylation 3 (alpha-1,3-manno...	[more]
Alg3	3.172e-62	56.82	asparagine-linked glycosylation 3 (alpha-1,3-manno...	[more]
Alg3	2.506e-53	53.66	asparagine-linked glycosylation 3 (alpha-1,3-manno...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8K2A8\|ALG3_MOUSE	7.341e-105	53.37	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosy...	[more]
sp\|Q92685\|ALG3_HUMAN	3.967e-104	53.08	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosy...	[more]
sp\|Q55F69\|ALG3_DICDI	1.701e-89	43.20	Probable Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,...	[more]
sp\|P82149\|NT53_DROME	3.569e-72	53.88	Lethal(2)neighbour of tid protein 2 OS=Drosophila ...	[more]
sp\|Q27333\|NT56_DROME	4.119e-72	53.88	Lethal(2)neighbour of tid protein OS=Drosophila me...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A419QE81	3.088e-128	58.41	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1 OS=Clonor...	[more]
A0A4V3SEY5	9.607e-127	57.65	Uncharacterized protein OS=Opisthorchis felineus O...	[more]
A0A504YLI2	3.826e-125	56.05	Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosy...	[more]
A0A074ZDK6	3.459e-124	56.98	Uncharacterized protein OS=Opisthorchis viverrini ...	[more]
A0A4Z2CND4	3.869e-124	59.52	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannos...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
alg3	1.052e-114	54.12	ALG3 alpha-1,3- mannosyltransferase [Source:NCBI g...	[more]
alg3	4.113e-114	54.12	ALG3 alpha-1,3- mannosyltransferase [Source:NCBI g...	[more]
alg3	3.666e-113	54.85	ALG3 alpha-1,3- mannosyltransferase [Source:NCBI g...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
alg3	4.593e-94	48.90	ALG3 alpha-1,3- mannosyltransferase [Source:ZFIN;A...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
ALG3	3.885e-52	37.64	Dolichol-P-Man dependent alpha(1-3) mannosyltransf...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO46580	1.803e-120	55.88	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
alg3	4.976e-114	57.91	ALG3 alpha-1,3- mannosyltransferase [Source:NCBI g...	[more]

BLAST of Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000008818.1	5.574e-8	98.91	SMESG000008818.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30007572 ID=SMED30007572|Name=Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1, 3-mannosyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1300bp
TTTTAATATTAAACAAATAATTGTCTTTTATCCTTAAATTTTTCATTAAA
TCGAAAATCATTTTAGCTTAATGAGTTCTTTTTGCGAAAAGTTAGAATGG
ATATTCTATAATAAATCCTGGAAAAGAACTTTGATGCTTCTTTTACTTTT
TCTAGAAGCTGGATTGTCTTATTTTATTATACTAAAAATACAGAAATTGA
TTGGATTGCATACATGCAAGAAGTAGAAGGATTTATTAATGGAACTTTAG
ATTATGAAAAATTACAGGGACAAACTGGTCCGTTAGTATATCCCGCAGGA
TTTGTATACTTTTATAGTGCTTTATATTGGTTAACTGACAAAGGAACAAA
TATTTTATTTGCACAGTTTATATTTTTAGGATTTTATGTAATAACGTTAG
GATTAATATTTCGAATATATAATCAATTTACACATATCCCAAACTATGTT
TTCGTATTCATGTGTTGCACGTCATATAGAATTCATTCAATCTATATTCT
ACGTTTATTTAATGACCCAATTGCGATGATGTTTCTTTATGCAGCTATAA
ATTGCTTCATGAGTAACAGATATAGTTGGGGTTCAATTTTGTTTAGTATT
GGAGTTTCTATTAAAATGAATATTTTATTATTCGCTCCCGGATTATTATT
GATTTATCTATTTAATTGCGGGTTCTGGGAGACCGTCGGTCACCTATCTG
AGTGTGGAACTGTACAACTCGTTCTAGGAATGCCGTTCTTGATTCACAAT
TCTGCGCAATACCTAAACCGTGCGTTTGAATTCAAACGGCAATTTCTCTA
TAAATGGACCGTCAATTGGCGATTAATTCCGGAATCATTCTTTCTCAATC
GATATTTCCACCTTTTACTACTAGCTGTTCATTTGGTACTGCTATTAGTG
CTGGTGAGAAAGTTTATGAGGAACCAATCTCTGAAAAGCATTTCTGTCAC
GGCTAACAAAACAAATCTCGCGTTAGTTATGTTCATGAGTAATTTTATCG
GAATAGCTTGCAGTCGATCTCTCCACTATCAATTTTATATCTGGTATTAC
CATACCTTGCCCTTGATACTATGGTCATTACCGAATATTTCATCAATCTG
GCGATTGCTAATAATGGGTCTGATTGAACTATGCTGGAATACATATCCGT
CGACAATTTATAGCAGTGGGCTTTTACACTTGTGCCATCTTTTTGTTTTA
TTTCATTGGTTGAAAGGAAACGGTTTCAACTATATTACTAGTATGGTTGC
TATTAGTGAAACCAAGCAGAAACTTAAATAAAAATCATTCTTTTTTGTTG

protein sequence of SMED30007572-orf-1

>SMED30007572-orf-1 ID=SMED30007572-orf-1|Name=SMED30007572-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=356bp
MQEVEGFINGTLDYEKLQGQTGPLVYPAGFVYFYSALYWLTDKGTNILFA
QFIFLGFYVITLGLIFRIYNQFTHIPNYVFVFMCCTSYRIHSIYILRLFN
DPIAMMFLYAAINCFMSNRYSWGSILFSIGVSIKMNILLFAPGLLLIYLF
NCGFWETVGHLSECGTVQLVLGMPFLIHNSAQYLNRAFEFKRQFLYKWTV
NWRLIPESFFLNRYFHLLLLAVHLVLLLVLVRKFMRNQSLKSISVTANKT
NLALVMFMSNFIGIACSRSLHYQFYIWYYHTLPLILWSLPNISSIWRLLI
MGLIELCWNTYPSTIYSSGLLHLCHLFVLFHWLKGNGFNYITSMVAISET
KQKLK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0000030	mannosyltransferase activity
GO:0016758	transferase activity, transferring hexosyl groups
GO:0016740	transferase activity
GO:0016757	transferase activity, transferring glycosyl groups

Vocabulary: **cellular component**
Term	Definition
GO:0030176	integral component of endoplasmic reticulum membrane
GO:0016021	integral component of membrane
GO:0005783	endoplasmic reticulum
GO:0016020	membrane

Vocabulary: **biological process**
Term	Definition
GO:0097502	mannosylation

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002056	medial muscle cell

Vocabulary: **INTERPRO**
Term	Definition
IPR007873	Glycosyltransferase_ALG3

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007873	Glycosyltransferase, ALG3	PFAM	PF05208	ALG3	coord: 1..318 e-value: 9.9E-124 score: 413.4
IPR007873	Glycosyltransferase, ALG3	PANTHER	PTHR12646	NOT56 - RELATED	coord: 1..335
None	No IPR available	PANTHER	PTHR12646:SF0	DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE	coord: 1..335
None	No IPR available	TMHMM	TMhelix		coord: 24..41
None	No IPR available	TMHMM	TMhelix		coord: 311..333
None	No IPR available	TMHMM	TMhelix		coord: 90..112
None	No IPR available	TMHMM	TMhelix		coord: 48..70
None	No IPR available	TMHMM	TMhelix		coord: 209..231
None	No IPR available	TMHMM	TMhelix		coord: 125..147
None	No IPR available	TMHMM	TMhelix		coord: 274..296