DUF4515 domain-containing protein

Overview
NameDUF4515 domain-containing protein
Smed IDSMED30007557
Length (bp)1042
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DUF4515 domain-containing protein (SMED30007557) t-SNE clustered cells

Violin plots show distribution of expression levels for DUF4515 domain-containing protein (SMED30007557) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DUF4515 domain-containing protein (SMED30007557) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DUF4515 domain-containing protein (SMED30007557) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007557

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30007557h1SMcG0004703 SmedASXL_011449SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30007557h1SMcG0004703 dd_Smed_v6_13384_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30007557h1SMcG0004703 dd_Smed_v4_13384_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of DUF4515 domain-containing protein vs. TrEMBL
Match: A0A267FKG6 (DUF4515 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001659g1 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 4.015e-29
Identity = 78/253 (30.83%), Postives = 153/253 (60.47%), Query Frame = 3 
Query:  180 APTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFR 938
            AP      +   DKE  LKE+L  + KE+ED K ++ ++K +N+ L+ E ++ R E+  ++ Y+    ++R+  L+TL+DYNE  I+   + R EML+    +K +L+     K   ++  +DE+    E+  ++++Q + I+ LE + +N+R Q + +L+++K   + E ++FQ+ES +K++++++ AYKEA+ SL       + +NR+LR+++L  I+Q +LL E K +L  ++ +L +E +++ D    R
Sbjct:   27 APEVEQPQEEVVDKEQQLKEELAKVIKEVEDAKKKLSELKSENEWLQEESQRIRVESHEYMGYMEKRADKRQKALITLSDYNEHEIKVLLQQREEMLEAYSYQKEELKQTLLSKQNELSKARDELDELAEFKKLKDEQAEMIKHLEKEAQNLRIQHSEQLQQLKAKFMREKREFQEESEQKVHQLSKEAYKEAITSLIDHSNTVKLQNRKLRKDLLHMIQQTRLLQEHKRKLEIQHDELLKEQEFSKDIRKLR 279
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BLAST of DUF4515 domain-containing protein vs. TrEMBL
Match: A0A267FI61 (DUF4515 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001659g2 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.253e-29
Identity = 78/253 (30.83%), Postives = 153/253 (60.47%), Query Frame = 3 
Query:  180 APTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFR 938
            AP      +   DKE  LKE+L  + KE+ED K ++ ++K +N+ L+ E ++ R E+  ++ Y+    ++R+  L+TL+DYNE  I+   + R EML+    +K +L+     K   ++  +DE+    E+  ++++Q + I+ LE + +N+R Q + +L+++K   + E ++FQ+ES +K++++++ AYKEA+ SL       + +NR+LR+++L  I+Q +LL E K +L  ++ +L +E +++ D    R
Sbjct:   27 APEVEQPQEEVVDKEQQLKEELAKVIKEVEDAKKKLSELKSENEWLQEESQRIRVESHEYMGYMEKRADKRQKALITLSDYNEHEIKVLLQQREEMLEAYSYQKEELKQTLLSKQNELSKARDELDELAEFKKLKDEQAEMIKHLEKEAQNLRIQHSEQLQQLKAKFMREKREFQEESEQKVHQLSKEAYKEAITSLIDHSNTVKLQNRKLRKDLLHMIQQTRLLQEHKRKLEIQHDELLKEQEFSKDIRKLR 279
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BLAST of DUF4515 domain-containing protein vs. TrEMBL
Match: A0A267H0B3 (DUF4515 domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001659g4 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 4.051e-28
Identity = 78/253 (30.83%), Postives = 153/253 (60.47%), Query Frame = 3 
Query:  180 APTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFR 938
            AP      +   DKE  LKE+L  + KE+ED K ++ ++K +N+ L+ E ++ R E+  ++ Y+    ++R+  L+TL+DYNE  I+   + R EML+    +K +L+     K   ++  +DE+    E+  ++++Q + I+ LE + +N+R Q + +L+++K   + E ++FQ+ES +K++++++ AYKEA+ SL       + +NR+LR+++L  I+Q +LL E K +L  ++ +L +E +++ D    R
Sbjct:   47 APEGEQLQEEVVDKEQQLKEELAKVIKEVEDAKKKLSELKSENEWLQEESQRIRVESHEYMGYMEKRADKRQKALITLSDYNEHEIKVLLQQRDEMLEAYNYQKEELKQTLLSKQNELSKARDELDELAEFKKLKDEQAEMIKHLEKEAQNLRIQHSEQLQQLKAKFMREKREFQEESEQKVHQLSKEAYKEAITSLIDHSNTVKLQNRKLRKDLLHMIQQTRLLQEHKKKLEIQHDELLKEQEFSKDIRKLR 299
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BLAST of DUF4515 domain-containing protein vs. TrEMBL
Match: C3Z7V0 (DUF4515 domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_119043 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.397e-27
Identity = 76/259 (29.34%), Postives = 149/259 (57.53%), Query Frame = 3 
Query:  180 APTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFRQIRQEK 956
            AP+    +   ED+E LL ++L  +N E+ + K  +E+++++N+ L+ E  + R E+  +++Y+     +R++ +V+L+D N+  IE  ++ + E+L + E KK  L+ +  +++  +   K E++  DE+  ++  Q   I+DLE ++  +R   +  L+++K   + E   +Q ES+++I  + + A KEA   L +  +  + ENR+LR E+L  I++ + L+E K+ L  + ++L  E QYA D A  R  RQ K
Sbjct:   34 APSAEPPEG--EDREELLAKQLQSVNDELAEKKRRVEELRKENEWLQQEAHQTRVESHEYMSYMAKKTHKRQSTIVSLSDQNQKEIEKLQQEKSEVLAEYEQKKAGLRQLLLERENELAKTKKELQELDEFKKLQADQISRIKDLEREVLKMRGVHSDTLQQLKAQFLKEKSDYQHESDQRITSMGKLANKEAARCLDEHTQMIKLENRQLRHELLNLIKETRALHEHKLRLEDQRKELLREQQYAKDLAVLRSARQHK 290
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BLAST of DUF4515 domain-containing protein vs. TrEMBL
Match: A0A1S3HZW2 (uncharacterized protein LOC106159718 OS=Lingula unguis OX=7574 GN=LOC106159718 PE=4 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 4.314e-25
Identity = 74/247 (29.96%), Postives = 150/247 (60.73%), Query Frame = 3 
Query:  216 DKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFRQIRQEK 956
            +KE LLK++L  + +E+E  K ++++++++N+ L+ E  K R E+  ++NY+     +R++ +++L+D N+  I+   + +  ML++ E KK  L+ I  +K+  ++  K E+    EY  +++ Q  +I++LE +++ +R   +  ++K+K   + + ++F Q+S  +I+ +   A KEAL+ L++  K  + ENRELR+++   I++ + L+E K +L  + ++L  E QYA D    RQ RQ K
Sbjct:   32 EKEVLLKQELDNLTEELERVKKKVDEMRQENEWLQQEAHKVRVESHEYMNYMQKKTSKRQSLIISLSDQNQQEIQNILEQKANMLQEYEEKKAGLRSILMEKENLLSKTKQELEDLQEYKNLQKDQLSKIKELEKEVQKMRGVHSDTIQKLKSEFLKDKREFNQDSQNRISSLKAQADKEALQCLNEHTKRIKEENRELRKKLFRLIQKTRALHEHKKQLEEQKKELLREQQYARDLKKLRQTRQHK 278
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BLAST of DUF4515 domain-containing protein vs. Ensembl Cavefish
Match: ccdc166 (stress response protein nst1-like [Source:NCBI gene;Acc:103047699])

HSP 1 Score: 56.225 bits (134), Expect = 1.069e-8
Identity = 52/205 (25.37%), Postives = 106/205 (51.71%), Query Frame = 3 
Query:  291 KVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEE 905
            ++++ N+ L +E  K R ET+ +++ +    + R++ + TL D  +  +E   + R E L+K + +  +L+    KK+  +  LK EI    E+  +R++Q   I DLE +   +    A  L  +K   + E ++++ +  +K+    +AA KEA   L    +    EN+ LR+E+   I +   L ++K+ L++  RD +++
Sbjct:   39 QLRKDNEFLHNEIYKTRMETQEYVSCMFKQTQDRQSAVDTLKDQTQKKLEDLRRQREEALEKYQEQTTELKREILKKEHELALLKTEIAELKEFEGVRQQQLDRIADLEQEKAAMHQHHAESLAALKASFLKEKEQYEAQVKDKMQAFTQAADKEATHCLHSRTQEVSQENQLLRKELQQLISRACALRKKKL-LMTCCRDPQQQ 242
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BLAST of DUF4515 domain-containing protein vs. Ensembl Nematostella
Match: EDO44923 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUL4])

HSP 1 Score: 68.5514 bits (166), Expect = 6.658e-14
Identity = 42/135 (31.11%), Postives = 77/135 (57.04%), Query Frame = 3 
Query:  546 DEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFRQIRQ 950
             E+   +EY ++++ Q  EI  LE +++ +R + +  ++K+K   + E + +Q+ES+ KI E+A+ A +EA   L    +  + EN+ LR E+L  IR+ + L+E + EL ++++ L  E QY  D       RQ
Sbjct:    4 GELEDLEEYQVLQKDQEVEIARLEEEVQTMRRKHSEAIQKLKAQFLREKRGYQKESDSKIQEMAKQATQEAKHCLDDHTQKIKEENKVLRHELLMLIRRTRALHEHREELQAQHKALLREVQYGRDLKKLSGTRQ 138
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BLAST of DUF4515 domain-containing protein vs. Planmine SMEST
Match: SMESG000013289.1 (SMESG000013289.1)

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 3 
Query:  105 MPPXXXXXXXXXXXXXXXQEKDIENAPTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFRQIRQEKPKQLEEMNKNKQ 992
            MPPKGKKKSKKKGNTSGKQEKDIENAPTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKE LESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFRQI QEKPKQLEEMNKNKQ
Sbjct:    1 MPPKGKKKSKKKGNTSGKQEKDIENAPTTSDTDDIFEDKEALLKEKLHGINKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNLVTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEIRTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQQESNEKINEIARAAYKETLESLSKACKNTESENRELRQEMLWTIRQNKLLNERKIELLSENRDLKEEYQYALDFASFRQIGQEKPKQLEEMNKNKQ 296
The following BLAST results are available for this feature:
BLAST of DUF4515 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FKG64.015e-2930.83DUF4515 domain-containing protein OS=Macrostomum l... [more]
A0A267FI616.253e-2930.83DUF4515 domain-containing protein OS=Macrostomum l... [more]
A0A267H0B34.051e-2830.83DUF4515 domain-containing protein (Fragment) OS=Ma... [more]
C3Z7V01.397e-2729.34DUF4515 domain-containing protein OS=Branchiostoma... [more]
A0A1S3HZW24.314e-2529.96uncharacterized protein LOC106159718 OS=Lingula un... [more]
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BLAST of DUF4515 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc1661.069e-825.37stress response protein nst1-like [Source:NCBI gen... [more]
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BLAST of DUF4515 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO449236.658e-1431.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DUF4515 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4515 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000013289.10.000e+099.32SMESG000013289.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007557 ID=SMED30007557|Name=DUF4515 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1042bp
GCAACATTCAAATAATTTATTTGAATGAATTACTGAAAAGAATTTAATTA
ATTAATATTTGATTCAAAGACAAGAAAAATATTTTCAATTTATAAAAAAT
AAATATGCCACCAAAGGGAAAGAAAAAAAGTAAAAAGAAAGGAAATACTT
CTGGAAAGCAAGAAAAAGATATTGAAAATGCACCAACAACGTCTGATACT
GATGACATTTTTGAAGACAAAGAAGCCCTTCTAAAAGAAAAATTACACGG
AATAAATAAAGAAATTGAAGATACGAAATTGGAAATGGAAAAAGTAAAGG
AACAAAATAAAACTTTGGAATCTGAATGTGAAAAAGCCAGATGTGAAACG
GAAGTTTTCATAAATTATTTGAATGATTTAGAAGAGCAAAGAAAAACTAA
TTTGGTAACTCTCAATGATTATAATGAAAGCATGATTGAGTATTTCGAAA
AAATGAGACTTGAAATGTTAAAAAAATCTGAAAATAAAAAAAATGATTTA
CAAGTTATTTTTGAAAAAAAAGATAAAACTATCAATAATCTCAAGGATGA
AATACGAACATATGATGAATATTTGATAATTCGTGAAAAACAGCACAAAG
AAATTAGGGATCTGGAAAATCAAATTAAAAATATTCGTTGCCAGCAGGCG
GTTGAACTCGAGAAGATGAAAGTCCACCTAATCACAGAACATCAAAAGTT
CCAGCAGGAATCCAATGAAAAAATTAATGAAATCGCTAGGGCTGCTTACA
AAGAAGCACTGGAGAGTCTGTCCAAAGCATGTAAGAACACAGAATCAGAA
AATCGTGAACTAAGGCAAGAAATGTTATGGACAATCAGACAAAACAAATT
GTTAAATGAAAGAAAAATTGAATTGCTCAGTGAAAACAGAGATCTCAAAG
AAGAATATCAGTACGCATTGGATTTTGCATCATTTAGACAGATTCGTCAA
GAAAAACCGAAACAATTAGAAGAGATGAATAAAAATAAACAGTAGAAATA
ACTTGCAATTATTTTTTTTCTGTAATAAATTATCAAAGTCGG
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protein sequence of SMED30007557-orf-1

>SMED30007557-orf-1 ID=SMED30007557-orf-1|Name=SMED30007557-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=297bp
MPPKGKKKSKKKGNTSGKQEKDIENAPTTSDTDDIFEDKEALLKEKLHGI
NKEIEDTKLEMEKVKEQNKTLESECEKARCETEVFINYLNDLEEQRKTNL
VTLNDYNESMIEYFEKMRLEMLKKSENKKNDLQVIFEKKDKTINNLKDEI
RTYDEYLIIREKQHKEIRDLENQIKNIRCQQAVELEKMKVHLITEHQKFQ
QESNEKINEIARAAYKEALESLSKACKNTESENRELRQEMLWTIRQNKLL
NERKIELLSENRDLKEEYQYALDFASFRQIRQEKPKQLEEMNKNKQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR032777DUF4515
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 153..187
NoneNo IPR availableCOILSCoilCoilcoord: 47..81
NoneNo IPR availableCOILSCoilCoilcoord: 247..267
NoneNo IPR availableCOILSCoilCoilcoord: 212..239
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..37
NoneNo IPR availablePANTHERPTHR14845FAMILY NOT NAMEDcoord: 4..288
IPR032777Domain of unknown function DUF4515PFAMPF14988DUF4515coord: 81..271
e-value: 3.9E-15
score: 56.3