Protein LLP homolog
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007534 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Protein LLP homolog vs. Ensembl Mouse
Match: Llph (LLP homolog, long-term synaptic facilitation (Aplysia) [Source:MGI Symbol;Acc:MGI:1913475]) HSP 1 Score: 45.4394 bits (106), Expect = 2.410e-6 Identity = 38/105 (36.19%), Postives = 51/105 (48.57%), Query Frame = 1 Query: 31 MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSKLK------YPFITSKSFEESKSNEDVQEKNCXXXXXXXXXXXXXTNTKI-VKNLKNEHGNYPIWMN 324 MAK LRSK KRK++A +R +NAP L L VD L + +K +E E E C+ E ++ ET K K L ++HG YP+WMN Sbjct: 1 MAKSLRSKWKRKMRAEKRKKNAPRELNRLKSILRVDGDALMKDVEEIATVVVAKPRQEKMQCE---EGRCDGADEEKDDMKMETEIKRNRKTLLDQHGQYPVWMN 102
BLAST of Protein LLP homolog vs. Ensembl Mouse
Match: Llph (LLP homolog, long-term synaptic facilitation (Aplysia) [Source:MGI Symbol;Acc:MGI:1913475]) HSP 1 Score: 45.4394 bits (106), Expect = 2.410e-6 Identity = 38/105 (36.19%), Postives = 51/105 (48.57%), Query Frame = 1 Query: 31 MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSKLK------YPFITSKSFEESKSNEDVQEKNCXXXXXXXXXXXXXTNTKI-VKNLKNEHGNYPIWMN 324 MAK LRSK KRK++A +R +NAP L L VD L + +K +E E E C+ E ++ ET K K L ++HG YP+WMN Sbjct: 1 MAKSLRSKWKRKMRAEKRKKNAPRELNRLKSILRVDGDALMKDVEEIATVVVAKPRQEKMQCE---EGRCDGADEEKDDMKMETEIKRNRKTLLDQHGQYPVWMN 102
BLAST of Protein LLP homolog vs. TrEMBL
Match: A0A3S5ASF5 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS30091 PE=4 SV=1) HSP 1 Score: 58.151 bits (139), Expect = 7.149e-8 Identity = 38/98 (38.78%), Postives = 49/98 (50.00%), Query Frame = 1 Query: 31 MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSKLKYPFITSKSFEESKSNEDVQEKNCXXXXXXXXXXXXXTNTKIVKNLKNEHGNYPIWMN 324 MAK RSK KR +A+RRI+ P L K S++ S L P + K +E ++DV L N +I L+NEHGNYPIWMN Sbjct: 1 MAKSKRSKVKRHFRALRRIKRIPFELNRLKKIESINLSSLSMPSVNQKIIDEQCESKDVNP-------YSSDPLKSLENPQIKARLQNEHGNYPIWMN 91
BLAST of Protein LLP homolog vs. Planmine SMEST
Match: SMESG000010838.1 (SMESG000010838.1) HSP 1 Score: 164.466 bits (415), Expect = 4.519e-53 Identity = 97/98 (98.98%), Postives = 97/98 (98.98%), Query Frame = 1 Query: 31 MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSKLKYPFITSKSFEESKSNEDVQEKNCXXXXXXXXXXXXXTNTKIVKNLKNEHGNYPIWMN 324 MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSK KYPFITSKSFEESKSNEDVQEKNCEVPIEVVPELIEETNTKIVKNLKNEHGNYPIWMN Sbjct: 1 MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSKSKYPFITSKSFEESKSNEDVQEKNCEVPIEVVPELIEETNTKIVKNLKNEHGNYPIWMN 98 The following BLAST results are available for this feature:
BLAST of Protein LLP homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Protein LLP homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Protein LLP homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Protein LLP homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Protein LLP homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Protein LLP homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007534 ID=SMED30007534|Name=Protein LLP homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=486bpback to top protein sequence of SMED30007534-orf-1 >SMED30007534-orf-1 ID=SMED30007534-orf-1|Name=SMED30007534-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp MAKGLRSKSKRKVKAIRRIENAPIIRQTLLKTLSVDSSKLKYPFITSKSFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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