Ligand of Numb protein X 2

Overview
NameLigand of Numb protein X 2
Smed IDSMED30007523
Length (bp)721
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ligand of Numb protein X 2 (SMED30007523) t-SNE clustered cells

Violin plots show distribution of expression levels for Ligand of Numb protein X 2 (SMED30007523) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ligand of Numb protein X 2 (SMED30007523) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ligand of Numb protein X 2 (SMED30007523) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007523

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30007523h1SMcG0017999 dd_Smed_v6_10539_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
pharynx musculatureSMED30007523h1SMcG0017999 dd_Smed_v4_10539_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: TRAF4 (TNF receptor associated factor 4 [Source:HGNC Symbol;Acc:HGNC:12034])

HSP 1 Score: 50.0618 bits (118), Expect = 4.628e-7
Identity = 31/119 (26.05%), Postives = 57/119 (47.90%), Query Frame = 1 
Query:  124 DHKFEGVNEFNNELICKICQNLMINA-HNAPCGCRYCLECITTYLDSSDRFCPGNSVDCK-IELLNLHRNIYVDQPINNKISKVTVKC--PEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            D+KF  + +    L+C +C   M      + CG R+C  C+  +L      CP + +     +  + +  IY D  +  ++  + ++C   E  C ++  LR++Q H+  C+F  IPCP
Sbjct:    5 DYKF--LEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKFPHPYPQIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCP 121
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: TRAF4 (TNF receptor associated factor 4 [Source:HGNC Symbol;Acc:HGNC:12034])

HSP 1 Score: 50.0618 bits (118), Expect = 6.224e-7
Identity = 31/118 (26.27%), Postives = 55/118 (46.61%), Query Frame = 1 
Query:  124 DHKFEGVNEFNNELICKICQNLMINA-HNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKC--PEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            D+KF  + +    L+C +C   M      + CG R+C  C+  +L      CP + +         +  IY D  +  ++  + ++C   E  C ++  LR++Q H+  C+F  IPCP
Sbjct:    5 DYKF--LEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLD------YAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCP 114
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: TRAF6 (TNF receptor associated factor 6 [Source:HGNC Symbol;Acc:HGNC:12036])

HSP 1 Score: 50.8322 bits (120), Expect = 9.774e-7
Identity = 33/100 (33.00%), Postives = 47/100 (47.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            C IC   +  A   PCG R+C  CI   +  +   CP   VD +I L N    ++ D     +I  + VKCP   C    ELR++++H   C F  + CP
Sbjct:   70 CPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCP---VDNEILLEN---QLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: TRAF6 (TNF receptor associated factor 6 [Source:HGNC Symbol;Acc:HGNC:12036])

HSP 1 Score: 50.8322 bits (120), Expect = 9.774e-7
Identity = 33/100 (33.00%), Postives = 47/100 (47.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            C IC   +  A   PCG R+C  CI   +  +   CP   VD +I L N    ++ D     +I  + VKCP   C    ELR++++H   C F  + CP
Sbjct:   70 CPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCP---VDNEILLEN---QLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: TRAF4 (TNF receptor associated factor 4 [Source:HGNC Symbol;Acc:HGNC:12034])

HSP 1 Score: 50.0618 bits (118), Expect = 1.283e-6
Identity = 31/118 (26.27%), Postives = 55/118 (46.61%), Query Frame = 1 
Query:  124 DHKFEGVNEFNNELICKICQNLMINA-HNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKC--PEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            D+KF  + +    L+C +C   M      + CG R+C  C+  +L      CP + +         +  IY D  +  ++  + ++C   E  C ++  LR++Q H+  C+F  IPCP
Sbjct:    5 DYKF--LEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLD------YAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCP 114
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 56.9954 bits (136), Expect = 5.389e-9
Identity = 37/119 (31.09%), Postives = 58/119 (48.74%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEH 525
            C IC   + +A   PCG R+C  CI   +  + + CP ++     E+L L   ++ D     +I  +TVKC    C    ELR ++ H+  C F + PCP     C++  VP+  + EH
Sbjct:   71 CPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVDN-----EVL-LEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCPQ----CQE-SVPISHLDEH 178
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 56.9954 bits (136), Expect = 5.389e-9
Identity = 37/119 (31.09%), Postives = 58/119 (48.74%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEH 525
            C IC   + +A   PCG R+C  CI   +  + + CP ++     E+L L   ++ D     +I  +TVKC    C    ELR ++ H+  C F + PCP     C++  VP+  + EH
Sbjct:   71 CPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVDN-----EVL-LEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCPQ----CQE-SVPISHLDEH 178
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 56.9954 bits (136), Expect = 5.389e-9
Identity = 37/119 (31.09%), Postives = 58/119 (48.74%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEH 525
            C IC   + +A   PCG R+C  CI   +  + + CP ++     E+L L   ++ D     +I  +TVKC    C    ELR ++ H+  C F + PCP     C++  VP+  + EH
Sbjct:   71 CPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVDN-----EVL-LEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCPQ----CQE-SVPISHLDEH 178
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx1 (ligand of numb-protein X 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9439])

HSP 1 Score: 50.0618 bits (118), Expect = 1.056e-6
Identity = 27/93 (29.03%), Postives = 46/93 (49.46%), Query Frame = 1 
Query:  121 KDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSV-----DCKIELLNLHRNIYVDQPINNKISKVTVKCP 384
            ++H++    + +++L+C IC   +I   + PCG  YC EC+T +L  SD FCP +        C+   L +H+           + K+ V CP
Sbjct:   41 ENHEYTYKEDVDDDLMCHICLQPLIRPLDTPCGHTYCQECLTNFLLESD-FCPVDRTPLMLQKCRKSSLLVHK----------LLDKLMVSCP 122
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx2b (ligand of numb-protein X 2b [Source:ZFIN;Acc:ZDB-GENE-040426-2500])

HSP 1 Score: 50.0618 bits (118), Expect = 1.105e-6
Identity = 33/105 (31.43%), Postives = 56/105 (53.33%), Query Frame = 1 
Query:  127 HKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVD-CKIELLNLHRNIYVDQPINNKISKVTVKCP-EIQCEFNDELRYIQNHI 435
            H FE  +E ++EL+C IC   ++   + PCG  YC +C++ +L   D FCP   VD  +I+L     +  +   + N + K+ V CP   +CE + +   +Q H+
Sbjct:   32 HLFEYQDEVDDELVCHICLQPLLQPMDTPCGHTYCFQCLSNFLHDQD-FCP---VDRQRIQLQQCRASSLL---VRNLLDKLAVLCPFRSECELSMQRCELQPHL 129
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: sytl2 (synaptotagmin like 2 [Source:Xenbase;Acc:XB-GENE-945155])

HSP 1 Score: 51.9878 bits (123), Expect = 3.327e-7
Identity = 28/120 (23.33%), Postives = 57/120 (47.50%), Query Frame = 1 
Query:  118 MKDHKFEGVNEFNNELICKICQNLMINA-HNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKC--PEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            M  + ++ + +   + +C +C   M      + CG R+C  C+  +L      CP + +         +  IY D+ +  ++  ++++C   E  C ++ +L+ +Q H+ IC F  IPCP
Sbjct:    1 MPGYDYKFLEKLRRKFLCPLCGKAMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLD------YAKIYPDRDLETQVMGLSIRCIHSEEGCRWSGQLKQLQPHLNICAFNVIPCP 114
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: LNX1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:100487348])

HSP 1 Score: 50.447 bits (119), Expect = 9.988e-7
Identity = 29/88 (32.95%), Postives = 47/88 (53.41%), Query Frame = 1 
Query:  121 KDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCP 384
            ++H +    E +++L+C IC + +I   + PCG  YC  C+T++L   D FCP   VD K  LL   +   +   + N + K+ V CP
Sbjct:   30 ENHSYTYTEEVDDDLLCNICLHALIQPLDTPCGHTYCTLCLTSFLVHED-FCP---VDRKHILLQTCKKSTI--LVKNLLDKLPVICP 111
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: egf (epidermal growth factor [Source:Xenbase;Acc:XB-GENE-951823])

HSP 1 Score: 48.521 bits (114), Expect = 4.004e-6
Identity = 30/99 (30.30%), Postives = 46/99 (46.46%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            C IC   +  A   PCG R+C  CI   +  +   CP   VD +  + N    ++ D     +I  + VKCP   C    ELR+++ H+  C+F  + C
Sbjct:   72 CPICLMALREAVQTPCGHRFCKACILKSIRDAGHKCP---VDNESLMEN---QLFPDNFAKREILSLRVKCPSQGCTETMELRHLERHLVRCDFAGVEC 164
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Traf6 (TNF receptor-associated factor 6 [Source:MGI Symbol;Acc:MGI:108072])

HSP 1 Score: 51.9878 bits (123), Expect = 2.617e-7
Identity = 34/100 (34.00%), Postives = 48/100 (48.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            C IC   +  A   PCG R+C  CI   +  +   CP   VD +I L N    ++ D     +I  +TVKCP   C    ELR++++H   C F  + CP
Sbjct:   70 CPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCP---VDNEILLEN---QLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP 163
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Traf4 (TNF receptor associated factor 4 [Source:MGI Symbol;Acc:MGI:1202880])

HSP 1 Score: 50.0618 bits (118), Expect = 1.029e-6
Identity = 30/118 (25.42%), Postives = 55/118 (46.61%), Query Frame = 1 
Query:  124 DHKFEGVNEFNNELICKICQNLMINA-HNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKC--PEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            D+KF  + +    L+C +C   M      + CG R+C  C+  +L      CP + +         +  IY D  +  ++  + ++C   E  C ++  LR++Q H+  C+F  +PCP
Sbjct:    5 DYKF--LEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLD------YAKIYPDPELEVQVLGLAIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVVPCP 114
[Close]
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q6IWL4|TRAF6_DANRE (TNF receptor-associated factor 6 OS=Danio rerio OX=7955 GN=traf6 PE=1 SV=2)

HSP 1 Score: 56.9954 bits (136), Expect = 4.068e-8
Identity = 37/119 (31.09%), Postives = 58/119 (48.74%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEH 525
            C IC   + +A   PCG R+C  CI   +  + + CP ++     E+L L   ++ D     +I  +TVKC    C    ELR ++ H+  C F + PCP     C++  VP+  + EH
Sbjct:   71 CPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVDN-----EVL-LEEQLFPDNFAKREILSLTVKCSNFGCSEKMELRQLEKHLSQCRFATAPCPQ----CQE-SVPISHLDEH 178
[Close]
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q6DJN2|TRF6B_XENLA (TNF receptor-associated factor 6-B OS=Xenopus laevis OX=8355 GN=traf6-b PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.152e-7
Identity = 33/99 (33.33%), Postives = 47/99 (47.47%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            C IC   +  A   PCG R+C  CI   L  +   CP   VD +I + N    ++ D     +I  + VKCP   C    ELR+++ H+  C F S+ C
Sbjct:   72 CPICLMALREAVQTPCGHRFCKACIVKSLRDAGHKCP---VDNEILMEN---QLFPDNFAKREILSLKVKCPSQGCTETMELRHLERHLGQCQFASVEC 164
[Close]
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q3MV19|TRF6A_XENLA (TNF receptor-associated factor 6-A OS=Xenopus laevis OX=8355 GN=traf6-a PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.426e-7
Identity = 31/99 (31.31%), Postives = 51/99 (51.52%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            C IC   +  A   PCG R+C  CI   L ++   CP ++     E+L + + ++ D     +I  + VKCP + C    ELR++++H+  C F S+ C
Sbjct:   72 CPICLMALREAVQTPCGHRFCKACILKSLRNAGHKCPVDN-----EIL-MEKQLFPDNFAKREILSLRVKCPNLGCTDTMELRHLEHHLVQCQFASVEC 164
[Close]
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|A7XUJ6|TRAF6_PIG (TNF receptor-associated factor 6 OS=Sus scrofa OX=9823 GN=TRAF6 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.516e-7
Identity = 34/100 (34.00%), Postives = 49/100 (49.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            C IC   +  A   PCG R+C  CI   +  +   CP   VD +I L N    ++ D     +I  +TVKCP + C    ELR++++H   C F  + CP
Sbjct:   71 CPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCP---VDNEILLEN---QLFPDNFAKREILSLTVKCPNVGCLHKMELRHLEDHQAHCEFALMNCP 164
[Close]
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|B5DF45|TRAF6_RAT (TNF receptor-associated factor 6 OS=Rattus norvegicus OX=10116 GN=Traf6 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.004e-7
Identity = 35/100 (35.00%), Postives = 49/100 (49.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            C IC   +  A   PCG R+C  CIT  +  +   CP   VD +I L N    ++ D     +I  +TVKCP   C    ELR++++H   C F  + CP
Sbjct:   70 CPICLMALREAVQTPCGHRFCKACITKSIRDAGHKCP---VDNEILLEN---QLFPDNFAKREILSLTVKCPNKGCVQKMELRHLEDHQVHCEFALVICP 163
[Close]
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: I1ZID8 (TNF receptor associated factor-2 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.137e-33
Identity = 63/179 (35.20%), Postives = 105/179 (58.66%), Query Frame = 1 
Query:   91 LKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLK 627
            +  E++ +++  + FE      + L C +C+NL++NA  + CGCR+C +CI  Y+   + +CPG + +CK E L +  NI+ D  +N KI+K+ VKCPE  C+   EL  I+ H+R+C  +S  CP+F +GC   K+ +  +  HL      HS+L++D I N+RND   +     +L+
Sbjct:    1 MTAEKINRLLITYNFEVEGGVPDNLKCDLCKNLLVNACQSRCGCRFCSKCIMEYVSRGNLYCPGGTSECKEENLTICSNIHTDYRVNRKIAKMRVKCPEETCKVYCELVDIEEHLRLCTMKSQNCPFFVLGCNQPKMVIQDLTNHLQNGTFQHSKLIIDIISNMRNDFFGLTTKEVELR 179
[Close]
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: T1E101 (TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 2.298e-26
Identity = 57/187 (30.48%), Postives = 111/187 (59.36%), Query Frame = 1 
Query:   82 MVNLKYEELWKMMKDHKFEGVNE-FNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDV----DAMKKSIADLK 627
            M+  K + + +++  +  + V+E  NN+L+C +C  LM +A  + CGC YC  CI  YL  +  +CPG+  +CK + LNL  +++ +  ++ +IS++ VKCP I+C +  EL  ++ H+  C  +S+ CP   IGC++  +  D + +HL  ++  H++L++   + L++++    +  K+ I+ LK
Sbjct:    1 MIPSKLDNVQELLSKYNVKRVDESVNNDLLCILCNKLMRDACESACGCHYCYTCIRIYLSQNHNYCPGDKTECKEDYLNLEGDVFRNIRLSTRISRIVVKCPNIRCNYTSELLRLKEHLDKCTPESVECPCVTIGCEEEPLLKDEMAKHLTDQVLRHTQLIMSFCNELKSEINHNNETTKEDISHLK 187
[Close]
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: A0A0F7GWW3 (TNF receptor associated factor 4 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 4.099e-19
Identity = 47/125 (37.60%), Postives = 77/125 (61.60%), Query Frame = 1 
Query:  316 LHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRLNQR 690
            L+ NI VD P+N KI+K+ VKC E  C++ DELR +++H R C ++S+ CPY +IGC    +  D + +HL  EI  H++LL+D + N  N+++ +K +   L   N  L+  I++  L+   +R
Sbjct:   12 LNENICVDYPMNMKIAKLQVKCKEKSCDYVDELRKMEDHSRTCKYRSVACPYSNIGCDKCNISQDKVTDHLQSEIFVHTKLLMDFVVNFNNEMEMLKANNNGLSPVNELLRNEINELKLDGDRRR 136
[Close]
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: C3Y9A7 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_118654 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.508e-11
Identity = 52/204 (25.49%), Postives = 90/204 (44.12%), Query Frame = 1 
Query:  154 NNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQP----INNKISKVTVKCPEIQ--CEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVD-----AICEH-------------LDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRLNQRN 693
            + E IC IC+ +M +    PCG  +C +CI  +L S    CP           N  ++ ++ +P    + N IS +T++C   Q  CE   +L Y  NH   C++ S+PCP  + GC    + ++      +C++             L  E  GHS     CI  L+  ++  ++    L+ +N+ L+  +D+        RN
Sbjct:   14 DQEFICSICRCVMEDPQECPCGHVFCKDCIQQWLRSHST-CP-----------NCRKHCHMIKPVLPMVRNLISHLTIRCENHQAGCEKRVQLEYYDNHKLTCDYASVPCP--NEGCDTQVLRINMDDHRGVCDYHRETCLKGCGISILRKEQAGHS-----CILELKKKLEEQEQEKRSLQQQNAELQTRVDNLKFLCWRSRN 198
[Close]
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: T1DBK6 (TRAF-4 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.512e-10
Identity = 41/118 (34.75%), Postives = 67/118 (56.78%), Query Frame = 1 
Query:  112 KMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            K+ KD+K+      N+ L C +CQ+++++A    CGCRYC ECI  YL++ ++ C     DC+  + +    IY D+  N +IS + VKC    C F D L+ I+ H+  C +++  C
Sbjct:   14 KLYKDNKWRFT--INDNLKCVMCQDILLDAIQTDCGCRYCKECIDNYLENGEKECVAEYEDCRSVITSY---IY-DRATNKEISLLKVKCLYEGCVFEDTLKSIKGHLDFCEYRNEMC 125
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: traf1 (TNF receptor-associated factor 1-like [Source:NCBI gene;Acc:103045252])

HSP 1 Score: 58.151 bits (139), Expect = 1.756e-9
Identity = 48/186 (25.81%), Postives = 75/186 (40.32%), Query Frame = 1 
Query:  163 LICKICQNLMINAHNAPCGCRYCLECITTYL-DSSDRFC------PGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC--------------PYFDIGCKDHKVPVDAICEHLD-CEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKEN 654
             +C  C N++  AH   CG RYC  C+   L ++ D  C      PG+  D  + L   H   + D  IN +I ++ V C    C +   L+  ++H   C +  IPC               + + GC ++K      C  L   E++ HS     C +    D+   +K   D K E+   KE 
Sbjct:   27 FLCSSCNNVLNEAHQTQCGHRYCRACVMWLLRNNKDLICTKCKEDPGSESDSSMMLTLDH--FFSDNAINKEIQELRVHCVNQGCGWRSTLKDFEDHQSQCEYALIPCNIGCGLMVLRKTLATHLEKGCPNNKTTCQVCCSALTPAELQKHS-----CSNGTEKDLSKAEKRKKDSKQESPKRKET 205
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826])

HSP 1 Score: 56.9954 bits (136), Expect = 4.239e-9
Identity = 30/93 (32.26%), Postives = 50/93 (53.76%), Query Frame = 1 
Query:  121 KDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSV-----DCKIELLNLHRNIYVDQPINNKISKVTVKCP 384
            ++H++    E +++L+C IC   +I   + PCG  YC EC+T++L  SD FCP + V     +C+   L +H+           + K+TV CP
Sbjct:  270 ENHEYNYKEEADDDLLCHICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCPVDRVPLLLQNCRKSSLLVHK----------LLDKLTVGCP 351
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826])

HSP 1 Score: 56.9954 bits (136), Expect = 4.279e-9
Identity = 30/93 (32.26%), Postives = 50/93 (53.76%), Query Frame = 1 
Query:  121 KDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSV-----DCKIELLNLHRNIYVDQPINNKISKVTVKCP 384
            ++H++    E +++L+C IC   +I   + PCG  YC EC+T++L  SD FCP + V     +C+   L +H+           + K+TV CP
Sbjct:  270 ENHEYNYKEEADDDLLCHICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCPVDRVPLLLQNCRKSSLLVHK----------LLDKLTVGCP 351
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826])

HSP 1 Score: 56.9954 bits (136), Expect = 4.279e-9
Identity = 30/93 (32.26%), Postives = 50/93 (53.76%), Query Frame = 1 
Query:  121 KDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSV-----DCKIELLNLHRNIYVDQPINNKISKVTVKCP 384
            ++H++    E +++L+C IC   +I   + PCG  YC EC+T++L  SD FCP + V     +C+   L +H+           + K+TV CP
Sbjct:  270 ENHEYNYKEEADDDLLCHICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCPVDRVPLLLQNCRKSSLLVHK----------LLDKLTVGCP 351
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: traf6 (TNF receptor associated factor 6 [Source:NCBI gene;Acc:103021325])

HSP 1 Score: 53.9138 bits (128), Expect = 3.870e-8
Identity = 32/100 (32.00%), Postives = 52/100 (52.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCP 468
            C IC   + +A   PCG R+C  CI   +  + + CP ++     E+L     ++ D     +I  +TV+C  + C    ELR+++NHI  C F ++PCP
Sbjct:   70 CPICLMGLRSAVQTPCGHRFCNCCIRKSIRDTGQKCPVDN-----EVLT-EDQLFPDNFAKREILSLTVRCRNVGCMEKMELRHLENHIPHCKFATVPCP 163
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 49.2914 bits (116), Expect = 3.283e-7
Identity = 29/100 (29.00%), Postives = 42/100 (42.00%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRN-IYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            C IC   +      PCG R+C  CI   +   D         C ++   LH   ++ D     +I  +TV+CP   C    ELR +Q H   C +  +PC
Sbjct:   65 CPICLMALREPVQTPCGHRFCRACILHSMRLRD-----AGPKCPVDNAVLHEGQLFPDNFAKREILSLTVRCPNKPCGHRGELRALQEHQLRCEYAQVPC 159
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO41243 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z7])

HSP 1 Score: 51.6026 bits (122), Expect = 9.507e-8
Identity = 32/110 (29.09%), Postives = 51/110 (46.36%), Query Frame = 1 
Query:  136 EGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            E V     E  C+IC   +       CG R+C EC    LD + R           E L+L +++++D+     I    VKCP   C++  ELR ++ H R C+++ + C
Sbjct:   14 EFVIAVAEEYTCRICHLPLRVPVQTKCGHRFCKEC----LDEASRRKSRAECPMDREPLDLKKDVFLDKAAERTILSFLVKCPCKGCKWEAELRDLEAHARNCDYELVTC 119
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO48394 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJK8])

HSP 1 Score: 50.0618 bits (118), Expect = 3.080e-7
Identity = 32/106 (30.19%), Postives = 52/106 (49.06%), Query Frame = 1 
Query:  154 NNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSV-DCKIELLNLH-RNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            N+ELIC IC  ++       CG  YC  CI       +++C G  V  C ++   L    I+ D+  +  +  + VKC    C +  ELR  Q H++IC+ +++ C
Sbjct:   25 NDELICAICHMVVNEPVLTSCGHSYCKYCI-------EKWCNGKEVVTCPVDNEQLKPSQIFPDKKTDRIVLSLEVKCTNNPCRWKGELRQKQKHLKICSHKAVKC 123
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO37750 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SER6])

HSP 1 Score: 47.7506 bits (112), Expect = 1.779e-6
Identity = 36/121 (29.75%), Postives = 53/121 (43.80%), Query Frame = 1 
Query:  118 MKDHKFEGVNEFNNELICKICQNLMINA-HNAPCGCRYCLECITTYLDSSDRFCP--GNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQ--CEFNDELRYIQNHIRICNFQSIPC 465
            M  HK   V      L+C+IC   + NA   + CG R+C  C+   L  +   CP    ++D        +  +Y D   N  I  +TV C   Q  C++  +LR +Q H+  C F S  C
Sbjct:    1 MPGHKSFFVPAVRENLLCRICHLPVRNAVQVSVCGHRFCDSCLNHALGETRLRCPVDNTAID--------YSQVYADDVTNQMIMSLTVICDYYQAGCKWKGQLRKLQGHLAECKFVSADC 113
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:101156395])

HSP 1 Score: 55.0694 bits (131), Expect = 2.321e-8
Identity = 22/56 (39.29%), Postives = 38/56 (67.86%), Query Frame = 1 
Query:  112 KMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCP 279
            K+ ++H++   +E +++L+C+IC   +I   + PCG  YC EC+T++L  SD FCP
Sbjct:   47 KLEENHEYLYQDEVDDDLVCQICLQPLIRPLDTPCGHTYCQECLTSFLLESD-FCP 101
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: traf6 (TNF receptor associated factor 6 [Source:NCBI gene;Acc:101161132])

HSP 1 Score: 53.5286 bits (127), Expect = 5.979e-8
Identity = 30/99 (30.30%), Postives = 50/99 (50.51%), Query Frame = 1 
Query:  169 CKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPC 465
            C IC   + NA    CG R+C  CI   +  + + CP ++     E+L+    ++ D     +I  +TV+CP   C    ELR+++ H+  C F ++PC
Sbjct:   78 CPICLMALRNAIQTRCGHRFCKSCIEKSIRDTGQRCPVDN-----EMLS-EDQLFPDNFAKREILSLTVRCPNSDCGEKMELRHLEKHLAQCQFATVPC 170
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: ENSORLT00000010826.2 (E3 ubiquitin-protein ligase RBBP6 [Source:NCBI gene;Acc:101160962])

HSP 1 Score: 47.7506 bits (112), Expect = 5.786e-6
Identity = 29/69 (42.03%), Postives = 38/69 (55.07%), Query Frame = 1 
Query:  157 NELICKICQNLMINAHNAPC-GCRYCLECI-TTYLDSSDRFCPG-NSVDCKIELLNLHRNIYVDQPINN 354
            +EL+C IC +LM +A   PC G  YC ECI T  LDS +  C   N  D   +  NL  N ++ Q +NN
Sbjct:  219 DELLCPICNDLMTDAVVIPCCGNSYCDECIRTALLDSEEHICSSCNQSDVSPD--NLIANKFLRQAVNN 285
[Close]
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: ENSORLT00000042274.1 (E3 ubiquitin-protein ligase RBBP6 [Source:NCBI gene;Acc:101160962])

HSP 1 Score: 47.7506 bits (112), Expect = 5.792e-6
Identity = 29/69 (42.03%), Postives = 38/69 (55.07%), Query Frame = 1 
Query:  157 NELICKICQNLMINAHNAPC-GCRYCLECI-TTYLDSSDRFCPG-NSVDCKIELLNLHRNIYVDQPINN 354
            +EL+C IC +LM +A   PC G  YC ECI T  LDS +  C   N  D   +  NL  N ++ Q +NN
Sbjct:  219 DELLCPICNDLMTDAVVIPCCGNSYCDECIRTALLDSEEHICSSCNQSDVSPD--NLIANKFLRQAVNN 285
[Close]
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000001730.1 (SMESG000001730.1)

HSP 1 Score: 413.69 bits (1062), Expect = 5.091e-149
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 1 
Query:   82 MVNLKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRLNQRN 693
            MVNLKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRLNQRN
Sbjct:    1 MVNLKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRLNQRN 204
[Close]
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000067216.1 (SMESG000067216.1)

HSP 1 Score: 200.29 bits (508), Expect = 1.438e-61
Identity = 88/194 (45.36%), Postives = 134/194 (69.07%), Query Frame = 1 
Query:  100 EELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRL 681
            E LW+ MK   F      N+EL C IC++++INAH+ PCGC YC ECI TYL    RFCPGN+ +C+++ LN++ +I VD+  N  +SK+ VKCP   C F DELR I+NH+RICN +   CPYF+IGCK+ ++  + +  H+  EI  HS+LLL+ IDNLRN+V+++K+++  L++E   L E ++  +++++
Sbjct:    2 ESLWETMKKMAFVSCQPVNHELTCLICKSIVINAHSGPCGCLYCFECIVTYLSKGIRFCPGNTEECQVKTLNINEDIVVDRSSNVSVSKLVVKCPNKNCSFKDELRQIENHLRICNMKCEKCPYFEIGCKESEISTEELSGHILQEIVNHSKLLLESIDNLRNEVESLKRTVEKLQVEKKQLTEEVELVMIDKI 195
[Close]
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000026111.1 (SMESG000026111.1)

HSP 1 Score: 198.749 bits (504), Expect = 4.990e-61
Identity = 90/192 (46.88%), Postives = 134/192 (69.79%), Query Frame = 1 
Query:  100 EELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLN 675
            E +WK MK +  E V E  NE+ C +C+ +++NAH+APCGCR+CL CI+ YL     +CPG++ DCK+EL+++  NI VD+P+N +ISK+ VKCPEI CEF  ELR +++HIR+CN+QS  CPY +IGC   K+  D I +HL  EI  H++LL+D + N  N+++ +K +  +L+ EN  L+  I++  LN
Sbjct:    6 ENVWKFMKTYNLETVVEIENEMSCLLCKKVLVNAHSAPCGCRFCLTCISQYLCDRKIYCPGDTDDCKLELISMSENIIVDRPVNVRISKLIVKCPEILCEFQSELRKMEDHIRMCNYQSSQCPYSNIGCDKSKISSDKIADHLQSEILMHTKLLMDFVGNFHNEMNTLKTNNNELREENKLLRIEINELKLN 197
[Close]
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000020135.1 (SMESG000020135.1)

HSP 1 Score: 196.438 bits (498), Expect = 3.126e-60
Identity = 91/197 (46.19%), Postives = 137/197 (69.54%), Query Frame = 1 
Query:   82 MVNLKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLE-------NSYLKE 651
            M   K+E +WK+M+++ F GV E ++++IC +C  +MIN+HNAPCGCR+CL+CI+ YL+  +  CPGN  DCK+EL++L  NI +D+P+NNKIS++ +KCPE  C++ DELR I+ HI  CN Q+I CPY  IGC +  V +  + +HL  +I  H++LL+DCIDNL N+++ +K  + + K +        SYL E
Sbjct:    1 MAKFKFENIWKIMENYDFSGV-EVDSDMICGLCGKVMINSHNAPCGCRFCLDCISNYLNGRENLCPGNKDDCKLELISLEENIAIDRPMNNKISRLIIKCPENSCDYVDELRKIELHISQCNSQTIICPYIKIGCNESDVSLGKMPDHLRNDIHNHTKLLMDCIDNLNNEMNEIKIIVGNFKGQFDKNTNDQSYLAE 196
[Close]
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000014923.1 (SMESG000014923.1)

HSP 1 Score: 187.193 bits (474), Expect = 9.621e-57
Identity = 86/182 (47.25%), Postives = 128/182 (70.33%), Query Frame = 1 
Query:   82 MVNLKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYCLECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKCPEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLDCEIEGHSRLLLDCIDNLRNDVDAMKKSIADLK 627
            M NL+YE +W+++ +++   + E +++LIC IC+NL+ NA N PCGCRYC ECI +YL   ++FCP N  +CK ELLN+ RNI +D   N++IS++ VKCPE  CEF+DELR I +HI  C+ + + CPYFDIGC + KV  + I +HL  +   H++ L++CI NL+N++  MK+   +LK
Sbjct:    1 MSNLRYENIWRILSEYRVNSMTEISSDLICVICKNLLKNACNGPCGCRYCFECIQSYLGQVEKFCPQNFDECKEELLNIDRNILLDNAANSRISRIIVKCPEPSCEFHDELRKIGDHINACDKKIVKCPYFDIGCSEGKVASERINDHLIVDNYTHAKTLVNCIQNLKNEMALMKECFEELK 182
The following BLAST results are available for this feature:
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRAF44.628e-726.05TNF receptor associated factor 4 [Source:HGNC Symb... [more]
TRAF46.224e-726.27TNF receptor associated factor 4 [Source:HGNC Symb... [more]
TRAF69.774e-733.00TNF receptor associated factor 6 [Source:HGNC Symb... [more]
TRAF69.774e-733.00TNF receptor associated factor 6 [Source:HGNC Symb... [more]
TRAF41.283e-626.27TNF receptor associated factor 4 [Source:HGNC Symb... [more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf65.389e-931.09TNF receptor-associated factor 6 [Source:NCBI gene... [more]
traf65.389e-931.09TNF receptor-associated factor 6 [Source:NCBI gene... [more]
traf65.389e-931.09TNF receptor-associated factor 6 [Source:NCBI gene... [more]
lnx11.056e-629.03ligand of numb-protein X 1 [Source:ZFIN;Acc:ZDB-GE... [more]
lnx2b1.105e-631.43ligand of numb-protein X 2b [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
sytl23.327e-723.33synaptotagmin like 2 [Source:Xenbase;Acc:XB-GENE-9... [more]
LNX19.988e-732.95ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
egf4.004e-630.30epidermal growth factor [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Traf62.617e-734.00TNF receptor-associated factor 6 [Source:MGI Symbo... [more]
Traf41.029e-625.42TNF receptor associated factor 4 [Source:MGI Symbo... [more]
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BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6IWL4|TRAF6_DANRE4.068e-831.09TNF receptor-associated factor 6 OS=Danio rerio OX... [more]
sp|Q6DJN2|TRF6B_XENLA2.152e-733.33TNF receptor-associated factor 6-B OS=Xenopus laev... [more]
sp|Q3MV19|TRF6A_XENLA2.426e-731.31TNF receptor-associated factor 6-A OS=Xenopus laev... [more]
sp|A7XUJ6|TRAF6_PIG2.516e-734.00TNF receptor-associated factor 6 OS=Sus scrofa OX=... [more]
sp|B5DF45|TRAF6_RAT3.004e-735.00TNF receptor-associated factor 6 OS=Rattus norvegi... [more]
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BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZID81.137e-3335.20TNF receptor associated factor-2 (Fragment) OS=Sch... [more]
T1E1012.298e-2630.48TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
A0A0F7GWW34.099e-1937.60TNF receptor associated factor 4 (Fragment) OS=Sch... [more]
C3Y9A73.508e-1125.49Uncharacterized protein OS=Branchiostoma floridae ... [more]
T1DBK62.512e-1034.75TRAF-4 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf11.756e-925.81TNF receptor-associated factor 1-like [Source:NCBI... [more]
lnx14.239e-932.26ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
lnx14.279e-932.26ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
lnx14.279e-932.26ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
traf63.870e-832.00TNF receptor associated factor 6 [Source:NCBI gene... [more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
traf63.283e-729.00TNF receptor-associated factor 6 [Source:NCBI gene... [more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO412439.507e-829.09Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO483943.080e-730.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377501.779e-629.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
lnx12.321e-839.29ligand of numb-protein X 1 [Source:NCBI gene;Acc:1... [more]
traf65.979e-830.30TNF receptor associated factor 6 [Source:NCBI gene... [more]
ENSORLT00000010826.25.786e-642.03E3 ubiquitin-protein ligase RBBP6 [Source:NCBI gen... [more]
ENSORLT00000042274.15.792e-642.03E3 ubiquitin-protein ligase RBBP6 [Source:NCBI gen... [more]
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BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000001730.15.091e-149100.00SMESG000001730.1[more]
SMESG000067216.11.438e-6145.36SMESG000067216.1[more]
SMESG000026111.14.990e-6146.88SMESG000026111.1[more]
SMESG000020135.13.126e-6046.19SMESG000020135.1[more]
SMESG000014923.19.621e-5747.25SMESG000014923.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007523 ID=SMED30007523|Name=Ligand of Numb protein X 2|organism=Schmidtea mediterranea sexual|type=transcript|length=721bp
AAAATATGAGTATTAATTTTAAGTTGTATTATTTTAATTTCGTCTTTCTA
TTTTATAAATTTTTTATATAATAACAAATCAATGGTGAATCTAAAATATG
AGGAATTATGGAAAATGATGAAAGACCATAAATTTGAAGGAGTCAATGAA
TTTAATAATGAATTAATTTGTAAAATATGTCAGAATCTCATGATTAATGC
GCATAATGCTCCGTGTGGTTGTCGTTATTGCTTAGAGTGCATAACGACGT
ATTTAGATAGTTCCGATAGATTTTGTCCTGGAAATTCAGTGGATTGTAAG
ATTGAGTTGTTAAATTTACACAGAAATATTTATGTTGATCAACCAATTAA
TAATAAAATATCAAAAGTAACAGTCAAGTGCCCTGAAATACAGTGTGAAT
TTAATGATGAATTGAGGTACATTCAAAATCATATCAGAATTTGTAACTTT
CAATCTATACCATGTCCATACTTCGATATAGGATGCAAGGATCACAAAGT
TCCTGTAGATGCTATTTGTGAACATCTAGACTGTGAAATTGAGGGTCATT
CTCGATTATTATTGGATTGTATTGATAATCTCAGAAATGATGTTGACGCT
ATGAAAAAAAGTATAGCTGATCTGAAATTAGAGAACAGTTATTTAAAAGA
AAATATAGACGACTTTGTTTTAAACCGACTTAATCAAAGAAATTAGTACA
ATGAAATTTTTTAGGTTTAAA
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protein sequence of SMED30007523-orf-1

>SMED30007523-orf-1 ID=SMED30007523-orf-1|Name=SMED30007523-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=205bp
MVNLKYEELWKMMKDHKFEGVNEFNNELICKICQNLMINAHNAPCGCRYC
LECITTYLDSSDRFCPGNSVDCKIELLNLHRNIYVDQPINNKISKVTVKC
PEIQCEFNDELRYIQNHIRICNFQSIPCPYFDIGCKDHKVPVDAICEHLD
CEIEGHSRLLLDCIDNLRNDVDAMKKSIADLKLENSYLKENIDDFVLNRL
NQRN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0031017exocrine pancreas development
GO:0070936protein K48-linked ubiquitination
GO:0006955immune response
GO:0007165signal transduction
GO:0016567protein ubiquitination
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
GO:0004842ubiquitin-protein transferase activity
GO:0046872metal ion binding