IQ domain-containing protein E-like

Overview
NameIQ domain-containing protein E-like
Smed IDSMED30007517
Length (bp)1283
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of IQ domain-containing protein E-like (SMED30007517) t-SNE clustered cells

Violin plots show distribution of expression levels for IQ domain-containing protein E-like (SMED30007517) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of IQ domain-containing protein E-like (SMED30007517) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for IQ domain-containing protein E-like (SMED30007517) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007517

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30007517SMESG000013899.1 SMESG000013896.1 SmedASXL_014741SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v6_7345_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30007517SMESG000013899.1 SMESG000013896.1 dd_Smed_v4_7345_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Human
Match: IQCE (IQ motif containing E [Source:HGNC Symbol;Acc:HGNC:29171])

HSP 1 Score: 63.5438 bits (153), Expect = 3.997e-10
Identity = 44/202 (21.78%), Postives = 104/202 (51.49%), Query Frame = 3 
Query:   21 FISMRKSDSNSYELWLDFINHKPGTFSK-FSSPNLKQYENNKINA-------EDF-----GLQKSETNSKMKH---YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
             IS+ ++     +LWL     KPG+ ++  +SP   ++    +         + F      L++S +N  +     Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++      + +R ++    +   +I  L+Q I +++ + K+K+  I  ++++ +   + E+++  E Y +E+  L+  
Sbjct:   15 LISLSRASLTPQKLWLG--TAKPGSLTQALNSPLTWEHAWTGVPGGTPDCLTDTFRVKRPHLRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGTDFVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTL 214
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Human
Match: IQCE (IQ motif containing E [Source:HGNC Symbol;Acc:HGNC:29171])

HSP 1 Score: 60.077 bits (144), Expect = 5.225e-9
Identity = 42/189 (22.22%), Postives = 98/189 (51.85%), Query Frame = 3 
Query:   60 LWLDFINHKPGTFSK-FSSPNLKQYENNKINA-------EDF-----GLQKSETNSKMKH---YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            LWL     KPG+ ++  +SP   ++    +         + F      L++S +N  +     Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++      + +R ++    +   +I  L+Q I +++ + K+K+  I  ++++ +   + E+++  E Y +E+  L+  
Sbjct:   79 LWLG--TAKPGSLTQALNSPLTWEHAWTGVPGGTPDCLTDTFRVKRPHLRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGTDFVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTL 265
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Human
Match: IQCE (IQ motif containing E [Source:HGNC Symbol;Acc:HGNC:29171])

HSP 1 Score: 59.6918 bits (143), Expect = 5.644e-9
Identity = 42/189 (22.22%), Postives = 98/189 (51.85%), Query Frame = 3 
Query:   60 LWLDFINHKPGTFSK-FSSPNLKQYENNKINA-------EDF-----GLQKSETNSKMKH---YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            LWL     KPG+ ++  +SP   ++    +         + F      L++S +N  +     Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++      + +R ++    +   +I  L+Q I +++ + K+K+  I  ++++ +   + E+++  E Y +E+  L+  
Sbjct:   63 LWLG--TAKPGSLTQALNSPLTWEHAWTGVPGGTPDCLTDTFRVKRPHLRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGTDFVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTL 249
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Human
Match: IQCE (IQ motif containing E [Source:HGNC Symbol;Acc:HGNC:29171])

HSP 1 Score: 59.6918 bits (143), Expect = 6.589e-9
Identity = 33/141 (23.40%), Postives = 81/141 (57.45%), Query Frame = 3 
Query:  165 LQKSETNSKMKH---YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            L++S +N  +     Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++      + +R ++    +   +I  L+Q I +++ + K+K+  I  ++++ +   + E+++  E Y +E+  L+  
Sbjct:  125 LRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGTDFVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTL 265
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Human
Match: IQCE (IQ motif containing E [Source:HGNC Symbol;Acc:HGNC:29171])

HSP 1 Score: 59.6918 bits (143), Expect = 7.179e-9
Identity = 42/189 (22.22%), Postives = 98/189 (51.85%), Query Frame = 3 
Query:   60 LWLDFINHKPGTFSK-FSSPNLKQYENNKINA-------EDF-----GLQKSETNSKMKH---YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            LWL     KPG+ ++  +SP   ++    +         + F      L++S +N  +     Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++      + +R ++    +   +I  L+Q I +++ + K+K+  I  ++++ +   + E+++  E Y +E+  L+  
Sbjct:   63 LWLG--TAKPGSLTQALNSPLTWEHAWTGVPGGTPDCLTDTFRVKRPHLRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGTDFVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTL 249
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Zebrafish
Match: iqce (IQ motif containing E [Source:ZFIN;Acc:ZDB-GENE-090312-217])

HSP 1 Score: 71.2478 bits (173), Expect = 7.683e-13
Identity = 51/190 (26.84%), Postives = 94/190 (49.47%), Query Frame = 3 
Query:   57 ELWLDFINHKPGTFSK-------------FSSPNLKQYENNKINAEDFGLQKSETNS---KMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIR---DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            ++WL   +   G+ SK              SS    +Y    +  +     +S +N        YK  EE YD+II  KK I   K E+D L++KLR+ EE   KKD+++++++   ++P   R   D   +   +I  L+Q I +++ + K+KEN +  ++S+ +   + E+K+  E Y +E++ L+  
Sbjct:   88 DVWLHLQDDGHGSLSKSPKVMDCSVTQSSISSARTPEYLKEALGMKKPKYSRSASNGYVPGTPDYKDKEEMYDEIIDLKKTIQTQKAESDKLRAKLRRFEEENCKKDRQIEQLLDPAKDPEYARSLVDRKTDPRSIINGLKQRILRLEQQCKEKENALSQLQSDHKTTNMQEMKITVETYFEEVQRLRTL 277
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Zebrafish
Match: iqce (IQ motif containing E [Source:ZFIN;Acc:ZDB-GENE-090312-217])

HSP 1 Score: 71.2478 bits (173), Expect = 8.167e-13
Identity = 51/190 (26.84%), Postives = 94/190 (49.47%), Query Frame = 3 
Query:   57 ELWLDFINHKPGTFSK-------------FSSPNLKQYENNKINAEDFGLQKSETNS---KMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIR---DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            ++WL   +   G+ SK              SS    +Y    +  +     +S +N        YK  EE YD+II  KK I   K E+D L++KLR+ EE   KKD+++++++   ++P   R   D   +   +I  L+Q I +++ + K+KEN +  ++S+ +   + E+K+  E Y +E++ L+  
Sbjct:   87 DVWLHLQDDGHGSLSKSPKVMDCSVTQSSISSARTPEYLKEALGMKKPKYSRSASNGYVPGTPDYKDKEEMYDEIIDLKKTIQTQKAESDKLRAKLRRFEEENCKKDRQIEQLLDPAKDPEYARSLVDRKTDPRSIINGLKQRILRLEQQCKEKENALSQLQSDHKTTNMQEMKITVETYFEEVQRLRTL 276
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Xenopus
Match: aldh16a1 (aldehyde dehydrogenase 16 family member A1 [Source:Xenbase;Acc:XB-GENE-5726619])

HSP 1 Score: 66.6254 bits (161), Expect = 4.134e-11
Identity = 37/126 (29.37%), Postives = 76/126 (60.32%), Query Frame = 3 
Query:  201 YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIR---DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            YK  E+ YD II  KK +   K E DI+K+KLR+ EE  N+KD+++++++    + +  R   D   ++ +++  L+Q + +++ + K+K++ +  ++SE +   + E+K+  E Y +EI+ L+  
Sbjct:  152 YKEKEDMYDQIIDLKKMLQAQKTEADIMKTKLRRLEEENNRKDRQIEQLLDPSRSSDFARSLVDTKSDSSLVVNNLRQKVLKLEKQCKEKDSALSKLQSELKTTSLEEMKIAMETYYEEIQRLQTL 277
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Mouse
Match: Iqce (IQ motif containing E [Source:MGI Symbol;Acc:MGI:1921489])

HSP 1 Score: 72.4034 bits (176), Expect = 3.680e-13
Identity = 47/189 (24.87%), Postives = 100/189 (52.91%), Query Frame = 3 
Query:   60 LWLDFINHKPGTFSKFSSPNLKQ-------------YENNKINAEDFGLQKSETN---SKMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            LWL   + K G+ ++  SP L               Y    + A+   L++S ++   S    Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++     P+ +R ++    +   +I  L+Q IF+++ + K+K+N I  ++++ +   + E+++  E Y +EI  L+  
Sbjct:   79 LWLG--SSKQGSVAQPPSPTLTSEHAWTHPPSCTPDYLTEAVRAKRADLRRSGSHGHVSGTSVYREKEDMYDEIIELKKSLHMQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGPDFVRTLAEKKPDTGWVITGLKQRIFRLEQQCKEKDNTINKLQTDMKTTNLEEMRIAMETYYEEIHRLQTL 265
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Mouse
Match: Iqce (IQ motif containing E [Source:MGI Symbol;Acc:MGI:1921489])

HSP 1 Score: 68.5514 bits (166), Expect = 7.806e-12
Identity = 34/126 (26.98%), Postives = 77/126 (61.11%), Query Frame = 3 
Query:  201 YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++     P+ +R ++    +   +I  L+Q IF+++ + K+K+N I  ++++ +   + E+++  E Y +EI  L+  
Sbjct:   95 YREKEDMYDEIIELKKSLHMQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGPDFVRTLAEKKPDTGWVITGLKQRIFRLEQQCKEKDNTINKLQTDMKTTNLEEMRIAMETYYEEIHRLQTL 220
[Close]
BLAST of IQ domain-containing protein E-like vs. UniProt/SwissProt
Match: sp|Q6PCQ0|IQCE_MOUSE (IQ domain-containing protein E OS=Mus musculus OX=10090 GN=Iqce PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.574e-12
Identity = 47/189 (24.87%), Postives = 100/189 (52.91%), Query Frame = 3 
Query:   60 LWLDFINHKPGTFSKFSSPNLKQ-------------YENNKINAEDFGLQKSETN---SKMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            LWL   + K G+ ++  SP L               Y    + A+   L++S ++   S    Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++     P+ +R ++    +   +I  L+Q IF+++ + K+K+N I  ++++ +   + E+++  E Y +EI  L+  
Sbjct:   79 LWLG--SSKQGSVAQPPSPTLTSEHAWTHPPSCTPDYLTEAVRAKRADLRRSGSHGHVSGTSVYREKEDMYDEIIELKKSLHMQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGPDFVRTLAEKKPDTGWVITGLKQRIFRLEQQCKEKDNTINKLQTDMKTTNLEEMRIAMETYYEEIHRLQTL 265
[Close]
BLAST of IQ domain-containing protein E-like vs. UniProt/SwissProt
Match: sp|Q6IPM2|IQCE_HUMAN (IQ domain-containing protein E OS=Homo sapiens OX=9606 GN=IQCE PE=1 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 3.164e-8
Identity = 33/141 (23.40%), Postives = 81/141 (57.45%), Query Frame = 3 
Query:  165 LQKSETNSKMKH---YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVS---VNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            L++S +N  +     Y+  E+ YD+II  KK +   K + D++++KLR+ EE  ++KD+++++++      + +R ++    +   +I  L+Q I +++ + K+K+  I  ++++ +   + E+++  E Y +E+  L+  
Sbjct:  125 LRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGTDFVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTL 265
[Close]
BLAST of IQ domain-containing protein E-like vs. TrEMBL
Match: A0A2B4RXX7 (IQ domain-containing protein E OS=Stylophora pistillata OX=50429 GN=Iqce PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.318e-12
Identity = 49/141 (34.75%), Postives = 82/141 (58.16%), Query Frame = 3 
Query:  201 YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRK--SEEILNKKDKELKEVIQCFENPNIIR----DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFNTNFQYSTLNNS 605
            YK  E+YYD+II  KK I  LK EN+IL +KLR+   E +L  KD+++++++   + P  +R    D   +   ++  L+Q +  ++  +KDKE E+  +K + +   I EL++Q+E Y QE++ L+      Q S LN S
Sbjct:  188 YKPQEDYYDEIIELKKVINTLKAENNILNTKLRRVEGENVL--KDRKMEDLLNTRKQPEDVRRTLTDKKGDTSAIVNSLKQKLHAVERTVKDKEAELSKLKKDIKMTNIEELQIQSETYYQEVQRLQLALIEMQQSQLNGS 326
[Close]
BLAST of IQ domain-containing protein E-like vs. TrEMBL
Match: A0A1I8HGL8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 7.890e-12
Identity = 70/313 (22.36%), Postives = 146/313 (46.65%), Query Frame = 3 
Query:  198 HYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVSVNN---YVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFN------TNFQYSTLNNSNDVAPHPSGRK----SSKSLPLHEKYKALMKKNKEISMXXXXXXXXXXXXXXXXSKEEVKSSGKNEEFVDEINRLKASHTKL--------------------IDEK--------TEMQSKLEATMLENKNLQKENEAKSKECEGLRKKFEKMNSKEVQKETKPKEVDK 1013
            +YK+ EEYYD++I  K+++  L+ E   +KSK R+ EE   K++K+L+ +    +N  + R ++  N    ++++ L+Q IF+++ +++DKE+ +  ++S+ +  K  E+K Q E+  +EI  L+  N      +N Q        +  P  + +K    ++    + ++  AL  +N ++      L+E+    +   +  E        + + +I  L+  + +L                    + EK        TE+ S+L A    NK L +E ++   + +  ++  E++N    +     KEVD+
Sbjct: 1671 NYKSAEEYYDEVIALKRELKALRAEMSSMKSKNRRLEEDYQKREKQLESLFDPTKNAELRRALADKNGESALVVQSLKQRIFKLEQQLRDKESALNKLQSDMKVTKTAEMKTQLEVLYKEIVRLRSANPADVLYSNEQPGGAGIDQEQMPKKTAQKLDALTATLRKVTDQKNALAAENAQLKKSEASLQEQLDELEGTTAIPESYRDMNRNQLLSKIRTLEQENDRLRVAPIGSEDIVKSKISLTGNVREKLELLDRRETELLSELAARTDANKRLMEERDSYKLQLQEAKRDIERLN---FENAGLSKEVDQ 1980
[Close]
BLAST of IQ domain-containing protein E-like vs. TrEMBL
Match: A0A1S3I136 (IQ domain-containing protein E-like OS=Lingula unguis OX=7574 GN=LOC106159708 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.985e-11
Identity = 42/143 (29.37%), Postives = 84/143 (58.74%), Query Frame = 3 
Query:  201 YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIR---DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFNTNFQYSTLNNSNDVAP 620
            YKTPEEYYD+I+  +K I  +  EN   K+++R+ EE   KK++E++ ++   ++  + R   D   +   +I  ++Q + +++ +++DKE+ +  ++++ +  K+ EL+LQ E + QE+  L+  NT    +T  +S    P
Sbjct:  168 YKTPEEYYDEILELRKQIAAISSENSTQKARVRRLEEDNLKKEREIETLLDPSKSEEMRRTLGDRKPDTGAVIHSMKQKVLKLEQQLRDKESNLTKLQADLKTTKVEELRLQMEAFYQEVVRLQ--NTRAAPATQQSSQSSKP 308
[Close]
BLAST of IQ domain-containing protein E-like vs. TrEMBL
Match: A0A1I8IZW7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.767e-10
Identity = 40/128 (31.25%), Postives = 80/128 (62.50%), Query Frame = 3 
Query:  198 HYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVSVNN---YVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFN 572
            +YK+ EEYYD++I  K+++  L+ E   +KSK R+ EE   K++K+L+ +    +N  + R ++  N    ++++ L+Q IF+++ +++DKE+ +  ++S+ +  K  E+K Q E+  +EI  L+  N
Sbjct:   99 NYKSAEEYYDEVIALKRELKALRAEMSSMKSKNRRLEEDYQKREKQLESLFDPTKNAELRRALADKNGESALVVQSLKQRIFKLEQQLRDKESALNKLQSDMKVTKTAEMKTQLEVLYKEIVRLRSAN 226
[Close]
BLAST of IQ domain-containing protein E-like vs. TrEMBL
Match: A0A267FFQ5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027365g1 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.811e-10
Identity = 40/128 (31.25%), Postives = 80/128 (62.50%), Query Frame = 3 
Query:  198 HYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVSVNN---YVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFN 572
            +YK+ EEYYD++I  K+++  L+ E   +KSK R+ EE   K++K+L+ +    +N  + R ++  N    ++++ L+Q IF+++ +++DKE+ +  ++S+ +  K  E+K Q E+  +EI  L+  N
Sbjct:   99 NYKSAEEYYDEVIALKRELKALRAEMSSMKSKNRRLEEDYQKREKQLESLFDPTKNAELRRALADKNGESALVVQSLKQRIFKLEQQLRDKESALNKLQSDMKVTKTAEMKTQLEVLYKEIVRLRSAN 226
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Medaka
Match: ENSORLT00000031579.1 (IQ motif containing E [Source:NCBI gene;Acc:101167345])

HSP 1 Score: 61.6178 bits (148), Expect = 7.203e-10
Identity = 35/126 (27.78%), Postives = 72/126 (57.14%), Query Frame = 3 
Query:  201 YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIR---DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            ++  E+ +D+II  KK +  L  +   +K+KLR+ EE + K++K+++E++   +     R   D      V++  L+Q I +++ + ++KEN +  ++SE R   + ELK+  + Y +EI+ L+  
Sbjct:  106 HRDKEDMHDEIIHLKKSLQALNSDKLQMKAKLRRLEEDIAKREKQIEELLDPIKGSEFTRSLVDKKREGSVVLNGLKQRILKLEQQCREKENALCKLQSELRTTNLEELKITAKTYSEEIQRLRML 231
[Close]
BLAST of IQ domain-containing protein E-like vs. Ensembl Medaka
Match: ENSORLT00000012070.2 (IQ motif containing E [Source:NCBI gene;Acc:101167345])

HSP 1 Score: 61.6178 bits (148), Expect = 7.203e-10
Identity = 35/126 (27.78%), Postives = 72/126 (57.14%), Query Frame = 3 
Query:  201 YKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIR---DVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQF 569
            ++  E+ +D+II  KK +  L  +   +K+KLR+ EE + K++K+++E++   +     R   D      V++  L+Q I +++ + ++KEN +  ++SE R   + ELK+  + Y +EI+ L+  
Sbjct:  106 HRDKEDMHDEIIHLKKSLQALNSDKLQMKAKLRRLEEDIAKREKQIEELLDPIKGSEFTRSLVDKKREGSVVLNGLKQRILKLEQQCREKENALCKLQSELRTTNLEELKITAKTYSEEIQRLRML 231
[Close]
BLAST of IQ domain-containing protein E-like vs. Planmine SMEST
Match: SMESG000013896.1 (SMESG000013896.1)

HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0
Identity = 309/312 (99.04%), Postives = 309/312 (99.04%), Query Frame = 3 
Query:   30 MRKSDSNSYELWLDFINHKPGTFSKFSSPNLKQYENNKINAEDFGLQKSETNSKMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVSVNNYVLIKKLQQNIFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFNTNFQYSTLNNSNDVAPHPSGRKSSKSLPLHEKYKALMKKNKEISMXXXXXXXXXXXXXXXXSKEEVKSSGKNEEFVDEINRLKASHTKLIDEKTEMQSKLEATMLENKNLQKENEAKSKECEGLRKKFEKM 965
            MRKSDSNSYELWLDFINHKPGTFSKFSSPNLKQYENNKINAEDFGLQKSE NSKMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVSVNNYVLIKKLQQN FQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFNTNFQYSTLNNSNDVAP PSGRKSSKSLPLHEKYKALMKKNKEISMENKLLKEKYKLKQNELSKEEVKSSGKNEEFVDEINRLKASHTKLIDEKTEMQSKLEATMLENKNLQKENEAKSKECEGLRKKFEKM
Sbjct:    1 MRKSDSNSYELWLDFINHKPGTFSKFSSPNLKQYENNKINAEDFGLQKSENNSKMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKDKELKEVIQCFENPNIIRDVSVNNYVLIKKLQQNFFQMKSEIKDKENEILTIKSETRYVKINELKLQNELYLQEIRSLKQFNTNFQYSTLNNSNDVAPRPSGRKSSKSLPLHEKYKALMKKNKEISMENKLLKEKYKLKQNELSKEEVKSSGKNEEFVDEINRLKASHTKLIDEKTEMQSKLEATMLENKNLQKENEAKSKECEGLRKKFEKM 312
[Close]
BLAST of IQ domain-containing protein E-like vs. Planmine SMEST
Match: SMESG000013899.1 (SMESG000013899.1)

HSP 1 Score: 139.813 bits (351), Expect = 1.367e-40
Identity = 66/66 (100.00%), Postives = 66/66 (100.00%), Query Frame = 3 
Query:  966 NSKEVQKETKPKEVDKNKPPENKNLLTVGMSCLAYLNRQKILDDYSTSEYPPNSKGMVLSAAVGFM 1163
            NSKEVQKETKPKEVDKNKPPENKNLLTVGMSCLAYLNRQKILDDYSTSEYPPNSKGMVLSAAVGFM
Sbjct:   10 NSKEVQKETKPKEVDKNKPPENKNLLTVGMSCLAYLNRQKILDDYSTSEYPPNSKGMVLSAAVGFM 75
The following BLAST results are available for this feature:
BLAST of IQ domain-containing protein E-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
IQCE3.997e-1021.78IQ motif containing E [Source:HGNC Symbol;Acc:HGNC... [more]
IQCE5.225e-922.22IQ motif containing E [Source:HGNC Symbol;Acc:HGNC... [more]
IQCE5.644e-922.22IQ motif containing E [Source:HGNC Symbol;Acc:HGNC... [more]
IQCE6.589e-923.40IQ motif containing E [Source:HGNC Symbol;Acc:HGNC... [more]
IQCE7.179e-922.22IQ motif containing E [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of IQ domain-containing protein E-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of IQ domain-containing protein E-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of IQ domain-containing protein E-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
iqce7.683e-1326.84IQ motif containing E [Source:ZFIN;Acc:ZDB-GENE-09... [more]
iqce8.167e-1326.84IQ motif containing E [Source:ZFIN;Acc:ZDB-GENE-09... [more]
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BLAST of IQ domain-containing protein E-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
aldh16a14.134e-1129.37aldehyde dehydrogenase 16 family member A1 [Source... [more]
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BLAST of IQ domain-containing protein E-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Iqce3.680e-1324.87IQ motif containing E [Source:MGI Symbol;Acc:MGI:1... [more]
Iqce7.806e-1226.98IQ motif containing E [Source:MGI Symbol;Acc:MGI:1... [more]
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BLAST of IQ domain-containing protein E-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q6PCQ0|IQCE_MOUSE2.574e-1224.87IQ domain-containing protein E OS=Mus musculus OX=... [more]
sp|Q6IPM2|IQCE_HUMAN3.164e-823.40IQ domain-containing protein E OS=Homo sapiens OX=... [more]
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BLAST of IQ domain-containing protein E-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2B4RXX75.318e-1234.75IQ domain-containing protein E OS=Stylophora pisti... [more]
A0A1I8HGL87.890e-1222.36Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1S3I1366.985e-1129.37IQ domain-containing protein E-like OS=Lingula ung... [more]
A0A1I8IZW72.767e-1031.25Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FFQ52.811e-1031.25Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of IQ domain-containing protein E-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of IQ domain-containing protein E-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of IQ domain-containing protein E-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of IQ domain-containing protein E-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of IQ domain-containing protein E-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000031579.17.203e-1027.78IQ motif containing E [Source:NCBI gene;Acc:101167... [more]
ENSORLT00000012070.27.203e-1027.78IQ motif containing E [Source:NCBI gene;Acc:101167... [more]
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BLAST of IQ domain-containing protein E-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000013896.10.000e+099.04SMESG000013896.1[more]
SMESG000013899.11.367e-40100.00SMESG000013899.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007517 ID=SMED30007517|Name=IQ domain-containing protein E-like|organism=Schmidtea mediterranea sexual|type=transcript|length=1283bp
CTATATAAATCTTATATGCATTTATTTCTATGCGGAAAAGTGATAGTAAT
AGTTATGAACTTTGGTTAGATTTTATAAATCATAAACCGGGTACTTTTTC
TAAATTTTCTTCTCCGAATCTAAAACAGTATGAAAATAATAAAATCAATG
CTGAGGATTTTGGTTTACAGAAAAGTGAAACTAATTCTAAAATGAAACAC
TATAAAACACCAGAAGAATACTACGATGATATTATTTTATTCAAGAAAGA
TATTGTGAAACTTAAAGGCGAAAATGACATTCTTAAATCTAAATTGCGTA
AAAGCGAGGAGATTTTAAATAAAAAGGATAAAGAACTGAAGGAAGTAATT
CAATGTTTCGAAAATCCAAATATTATTCGAGATGTGTCAGTTAACAATTA
TGTATTAATAAAAAAGCTACAACAAAATATTTTTCAAATGAAATCAGAAA
TAAAAGATAAAGAAAATGAAATTTTAACCATTAAATCGGAAACTCGGTAC
GTAAAAATAAATGAATTGAAATTGCAAAACGAATTGTACCTGCAAGAAAT
TAGAAGCTTAAAACAGTTTAATACCAATTTTCAATATTCTACATTAAATA
ATTCAAATGATGTTGCTCCTCATCCATCAGGTAGAAAATCATCGAAATCA
TTGCCGTTACATGAAAAATATAAAGCATTAATGAAAAAAAATAAGGAAAT
TTCAATGGAAAATAAATTGCTTAAAGAAAAGTATAAATTAAAACAAAACG
AACTGTCGAAAGAGGAAGTAAAATCTTCAGGAAAAAACGAAGAATTCGTT
GATGAAATTAATCGACTAAAAGCATCGCATACAAAATTAATTGACGAAAA
AACTGAAATGCAATCTAAATTGGAGGCTACTATGTTGGAGAACAAAAATT
TACAAAAAGAAAATGAAGCCAAATCAAAAGAATGTGAAGGATTGAGAAAA
AAATTTGAAAAAATGAATTCCAAAGAAGTGCAAAAGGAAACGAAACCAAA
AGAAGTAGATAAAAATAAACCTCCCGAAAATAAGAATCTCCTAACGGTTG
GAATGAGCTGTTTGGCCTATTTGAATCGGCAAAAAATTCTTGATGATTAT
TCAACCTCTGAATATCCCCCGAATTCTAAAGGCATGGTCTTGTCGGCGGC
AGTTGGATTCATGTTACGAGAAAAACATTTAAAACATTAAAAATATAAAA
AACGCTATGAAATACATTTATTGAAACAAAAAAAAATATTGCAACTAAAT
AGTCGTTTTTTTAATAAAATTACTTATCTTGAC
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protein sequence of SMED30007517-orf-1

>SMED30007517-orf-1 ID=SMED30007517-orf-1|Name=SMED30007517-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=387bp
MRKSDSNSYELWLDFINHKPGTFSKFSSPNLKQYENNKINAEDFGLQKSE
TNSKMKHYKTPEEYYDDIILFKKDIVKLKGENDILKSKLRKSEEILNKKD
KELKEVIQCFENPNIIRDVSVNNYVLIKKLQQNIFQMKSEIKDKENEILT
IKSETRYVKINELKLQNELYLQEIRSLKQFNTNFQYSTLNNSNDVAPHPS
GRKSSKSLPLHEKYKALMKKNKEISMENKLLKEKYKLKQNELSKEEVKSS
GKNEEFVDEINRLKASHTKLIDEKTEMQSKLEATMLENKNLQKENEAKSK
ECEGLRKKFEKMNSKEVQKETKPKEVDKNKPPENKNLLTVGMSCLAYLNR
QKILDDYSTSEYPPNSKGMVLSAAVGFMLREKHLKH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 218..252
NoneNo IPR availableCOILSCoilCoilcoord: 257..319
NoneNo IPR availableCOILSCoilCoilcoord: 79..106
NoneNo IPR availableCOILSCoilCoilcoord: 131..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 314..336
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 314..337
NoneNo IPR availablePANTHERPTHR14952:SF7IQ DOMAIN-CONTAINING PROTEIN Ecoord: 61..239
NoneNo IPR availablePANTHERPTHR14952FAMILY NOT NAMEDcoord: 61..239