Rep

Overview
NameRep
Smed IDSMED30007492
Length (bp)2363
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rep (SMED30007492) t-SNE clustered cells

Violin plots show distribution of expression levels for Rep (SMED30007492) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rep (SMED30007492) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rep (SMED30007492) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007492

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30007492 dd_Smed_v4_23932_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rep vs. TrEMBL
Match: Q77I17 (Rep OS=Planaria asexual strain-specific virus-like element type 1 OX=159252 GN=rep PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.340e-24
Identity = 72/235 (30.64%), Postives = 117/235 (49.79%), Query Frame = 2 
Query: 1577 GFRHMQMYVEVYTNMSVYAFRAKLNVLFVSGVAGPDRPHAIEYCTKSRSRIDNGV------RVRIEQPSNFTIKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFIKKKTIME-SLIQKFGINTKSNFVN----------FLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSVIDI 2230
            GFRH+Q+++      +      K  V FV  V   D    ++YCTK  +R++NGV      R R+ Q   F  K            LNV + +E + ++E+  P  +I+ +  +E +L+ K G  T++ F +          +  P+ D    +S +FVG+SGIGKTNFALSHF++P+HV   Q L ++       DG V DD++  ++ + E   ++DC F   + + Y    I
Sbjct:    5 GFRHIQLFITCKYATTASNVAKKFGVGFVGVVESHDVQKWVDYCTKVDTRVENGVTIKHGERARLSQDQEFWAK-----------FLNVNSEREGMEMVERERPLDYIRFREKLEGALLHKLGSVTQAKFKSGWRWPYYGFMYRDPETDEVSMRSWVFVGKSGIGKTNFALSHFEQPLHVTEKQELRKLGSTKFPYDGFVLDDVHLAEWFVGEAQNVLDCRFACTMRVLYGTAHI 228
[Close]
BLAST of Rep vs. TrEMBL
Match: Q910M2 (Rep OS=Planaria asexual strain-specific virus-like element type 2 OX=159253 GN=rep PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.340e-24
Identity = 72/235 (30.64%), Postives = 117/235 (49.79%), Query Frame = 2 
Query: 1577 GFRHMQMYVEVYTNMSVYAFRAKLNVLFVSGVAGPDRPHAIEYCTKSRSRIDNGV------RVRIEQPSNFTIKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFIKKKTIME-SLIQKFGINTKSNFVN----------FLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSVIDI 2230
            GFRH+Q+++      +      K  V FV  V   D    ++YCTK  +R++NGV      R R+ Q   F  K            LNV + +E + ++E+  P  +I+ +  +E +L+ K G  T++ F +          +  P+ D    +S +FVG+SGIGKTNFALSHF++P+HV   Q L ++       DG V DD++  ++ + E   ++DC F   + + Y    I
Sbjct:    5 GFRHIQLFITCKYATTASNVAKKFGVGFVGVVESHDVQKWVDYCTKVDTRVENGVTIKHGERARLSQDQEFWAK-----------FLNVNSEREGMEMVERERPLDYIRFREKLEGALLHKLGSVTQAKFKSGWRWPYYGFMYRDPETDEVSMRSWVFVGKSGIGKTNFALSHFEQPLHVTEKQELRKLGSTKFPYDGFVLDDVHLAEWFVGEAQNVLDCRFACTMRVLYGTAHI 228
[Close]
BLAST of Rep vs. TrEMBL
Match: A0A075LYR7 (Replication-associated protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-2 OX=1521386 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.120e-15
Identity = 65/254 (25.59%), Postives = 118/254 (46.46%), Query Frame = 2 
Query: 1514 EVFKDTEFTRFVVGQEEQGDDGFRHMQMYVEVYTNMSVYAFRAKLNVLFVSGVAGPDRPH----------AIEYCTKSRSRIDN-GVRVRIEQPSNFTIKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFI----KKKTIMESLIQKFGINTKSNFVNFLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSVIDI 2230
            E+ K  +   + +GQ E G +G  H QMY E  T +S+  ++  L         G  R H          AIEYC K  +R+   G      + +  ++K+ +     +  ALN  +  +++ I++   P  ++    +  + ++++ +     T     N      +  + ++ IF+G SG+GKT FA+SHFK P+ V H+  L ++     + DGI+FDD+ F  +     + L D      +N+KY  ++I
Sbjct:   19 ELQKWNQSLTYGIGQLELGLNGSTHWQMYFENNTAISLTQWKQLL---------GCKRAHVETRKGTALLAIEYCKKEETRLHGPGTSFEFGKTTEESVKRKSPD-SPYKKALNCLSYADSIAIIKDEAPRDYVIYHNQVTSTLKAIFKPVWNKTPGLVFNLEPFPEETLNTRAVIFMGRSGLGKTQFAISHFKDPLIVSHIDDLKKLN---PTIDGIIFDDMNFQHWPPTACIHLCDMELPRSINVKYGTVEI 259
[Close]
BLAST of Rep vs. TrEMBL
Match: V4AAM0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_172863 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.600e-15
Identity = 51/143 (35.66%), Postives = 77/143 (53.85%), Query Frame = -2 
Query:  305 VKYKGEMHVPGYNFCGPGTSTDLRLDQNGMPETTSLPVNAIDRLCVDHDKDYSNKNRQVRYQSDLRLIKALNDMDTTNMSKQDMLVRKLIIYVFKLKTNF----EVLAGLIKNIEADMKSIKLNMHDIELVKRFSDNIKVAQD 721
             KY GE H+PG+N+ GPGT  DLRLD+N  P+    PVN +D   + HD  Y NK+   R+Q+D ++I  L ++   N + ++ L R LII + K K         L  LI  ++  +  +K++      V+R  DN  V +D
Sbjct:   31 AKYPGEKHLPGHNYTGPGTRLDLRLDENDNPKPGEEPVNRVDAAALKHDILYRNKDITFRHQADKQMIDELENI--PNPTFKEKLQRALIIKLLKAKVKLVNKTSQLQNLISKLDDKITKVKID------VQRLIDNPNVNED 165
[Close]
BLAST of Rep vs. TrEMBL
Match: A0A5Q2F4S9 (Replication associated protein OS=Antarctic virus COCH21_51 OX=2664243 GN=rep PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 8.439e-15
Identity = 65/244 (26.64%), Postives = 114/244 (46.72%), Query Frame = 2 
Query: 1544 FVVGQEEQGDDGFRHMQMYVEVYTNMSVYAFRAKLNVLFVSGVAGPDRPH----------AIEYCTKSRSRIDNGVRVRIEQPSNFT-IKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFI----KKKTIMESLIQKFGINTKSNFVNFLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSVIDI 2230
            + +GQ E G +G  H QMY E  T +S+  ++  L         G  R H          AIEYC K  +R+  G     E  +    +KK       +  ALN  +  +++ I++   P  ++    +  + ++++ +     T     N      +  + ++ IF+G SG+GKT FA+SHFK+P+ V H+  L ++  ++   DGI+FDD+ F  +     + L D      +N+KY  ++I
Sbjct:   29 YGIGQLELGLNGSIHWQMYFENNTAISLTQWKQLL---------GSKRAHVETRKGTALLAIEYCKKEETRMQ-GPGTSFEFGNVMEELKKRKSPDSPYKKALNCLSYADSIAIIKDEAPRDYVIYHNQVTSTLKAIFKPVWNKTPGLVFNLEPFPEETLNTRAVIFMGRSGLGKTQFAISHFKEPLIVSHIDDLKKLNPNI---DGIIFDDMNFQHWPPTACIHLCDMELPRSINVKYGTVEI 259
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000055972.1 (SMESG000055972.1)

HSP 1 Score: 194.126 bits (492), Expect = 9.136e-57
Identity = 105/203 (51.72%), Postives = 135/203 (66.50%), Query Frame = 2 
Query: 1619 MSVYAFRAKLNVLFVSGVAGPDRPHAIEYCTKSRSRIDNGVRVRIEQPSNFTIKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFIKK-KTIMESLIQKFGINTKSNFV-NFLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSV 2221
            MSV+ FRA+  + FV G+AGPDR  A  Y TK   R+  G+ ++I Q + ++        DNF +AL  KT++EA+ IL   E FH++ K KTI+E+LI  FG +T  N   N L+PK++L DWKSH+F+G+S IGKT FALSHF KPVHVKHLQMLN +T ++  CDGIVF DLYFNK            A+EN VNIKYSV
Sbjct:    1 MSVFIFRARSGIPFVRGIAGPDRQWASLYSTKDDLRVLGGLSIKIVQLNKYS--SLVQATDNFKMALEAKTMKEALNILMTKESFHYMTKGKTILETLINIFGNSTTHNLSENVLIPKMNLLDWKSHVFLGDSVIGKTYFALSHFNKPVHVKHLQMLNIITSNVDECDGIVFHDLYFNK------------AYENVVNIKYSV 189
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000012349.1 (SMESG000012349.1)

HSP 1 Score: 176.407 bits (446), Expect = 7.256e-52
Identity = 83/107 (77.57%), Postives = 92/107 (85.98%), Query Frame = -2 
Query:  383 MHVPGYNFCGPGTSTDLRLDQNGMPETTSLPVNAIDRLCVDHDKDYSNKNRQVRYQSDLRLIKALNDMDTTNMSKQDMLVRKLIIYVFKLKTNFEVLAGLIKNIEAD 703
            MHVPGYNFCGPGT TDLRLD+N  P  TSLP+N+ID LCV HD +Y N+NR VRY+SDL+LIKAL DM T NMSKQD LVRKLIIYVFKLKTNFEVLAGLIK++E D
Sbjct:    1 MHVPGYNFCGPGTRTDLRLDKNKKPVPTSLPINSIDELCVAHDIEYGNQNRLVRYKSDLKLIKALQDMATENMSKQDKLVRKLIIYVFKLKTNFEVLAGLIKDVEKD 107
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000000949.1 (SMESG000000949.1)

HSP 1 Score: 108.997 bits (271), Expect = 1.853e-27
Identity = 59/145 (40.69%), Postives = 90/145 (62.07%), Query Frame = -2 
Query:  284 KYKGEMHVPGYNFCGPGTSTDLRLDQNGMPETTSLPVNAIDRLCVDHDKDYSNKNRQVRYQSDLRLIKALNDMDTTNMSKQDMLVRKLIIYVFKLKTNFEVLAGLIKNIEADMKSIKLNMHDIELVKRFSDNIKVAQDLRLFLKT 718
            K KGEMH+PGYNF GPGT TDLRLD++  P + S P+N ID  C+ HD  Y++K+++VRY SD +LI  ++ +   + + ++ +V  LIIYVF+LK  FE   G+++N         L   + E +K +  N++ +  +  FL T
Sbjct:    3 KLKGEMHIPGYNFWGPGTRTDLRLDEHKKPVSWSKPINKIDEQCLTHDIAYNSKDKRVRYDSDRQLI--MDILYLRHKTPEEEVVGNLIIYVFELKNIFEKKLGIVENQH-------LTKEEQEQMKNYVRNLEYSTTIAKFLGT 138
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000059230.1 (SMESG000059230.1)

HSP 1 Score: 107.071 bits (266), Expect = 1.819e-23
Identity = 63/171 (36.84%), Postives = 97/171 (56.73%), Query Frame = 2 
Query: 1721 SRIDNGVRVRIEQPSNFTIKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFIKKKTIMES-LIQKFGINTKSNFVNFLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSVIDI 2230
            +R+D GV + I  P   T+  T D    +   L +   +EA+  L++ +P  +I ++  +E+ LI++   N  + F +F  P ID+ D K+HIF+G +GIGKTNFAL+HFKKP+     Q   ++     S DG+V DD+ F  +     L +VDC F+  VN+KY V  I
Sbjct:  538 TRVDGGVVLSIGTPPK-TLPHTKDI---YRQLLQIDNPEEAMEFLKEKDPQTYILQRQKIEACLIREARRNVNAKFTSFNRPFIDVNDKKTHIFIGGTGIGKTNFALAHFKKPILCSDRQDFGKIGA---STDGLVIDDMTFRSWLPTNLLHIVDCEFDRSVNVKYGVSTI 701
[Close]
BLAST of Rep vs. Planmine SMEST
Match: SMESG000069836.1 (SMESG000069836.1)

HSP 1 Score: 76.6406 bits (187), Expect = 9.423e-16
Identity = 44/135 (32.59%), Postives = 77/135 (57.04%), Query Frame = 2 
Query: 1817 LNVKTIQEAVLILEKSEPFHFIKKKTIMESLIQKFGINTKSNFVNFLVPKIDLYDWKSHIFVGESGIGKTNFALSHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCAFENDVNIKYSV 2221
            L+  T+++ +  +E+  P  FI+       +IQ    N+K N   + +P  DL D ++ + +G +G+GKTN+ LSHFK P+HV   Q  +++  D    DG+V DD+  +K+  EE + L D  + + +N+KY +
Sbjct:   30 LSSSTVEDGMRRVEEENPLSFIQ-------IIQ----NSKFN-EGYRIPYQDLKD-RTLVLIGGTGVGKTNYTLSHFKDPLHVTIRQDFDKMGPDNHVYDGVVIDDVALSKWDPEEVIYLCDRNYSSSINVKYGI 151
The following BLAST results are available for this feature:
BLAST of Rep vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q77I172.340e-2430.64Rep OS=Planaria asexual strain-specific virus-like... [more]
Q910M22.340e-2430.64Rep OS=Planaria asexual strain-specific virus-like... [more]
A0A075LYR72.120e-1525.59Replication-associated protein OS=McMurdo Ice Shel... [more]
V4AAM05.600e-1535.66Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A5Q2F4S98.439e-1526.64Replication associated protein OS=Antarctic virus ... [more]
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BLAST of Rep vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rep vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000055972.19.136e-5751.72SMESG000055972.1[more]
SMESG000012349.17.256e-5277.57SMESG000012349.1[more]
SMESG000000949.11.853e-2740.69SMESG000000949.1[more]
SMESG000059230.11.819e-2336.84SMESG000059230.1[more]
SMESG000069836.19.423e-1632.59SMESG000069836.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007492 ID=SMED30007492|Name=Rep|organism=Schmidtea mediterranea sexual|type=transcript|length=2363bp
TTGGGGATGAGTCACAAAACATAAATTAAATTTATTATATTTAATTAACA
AAATTTAAGTTCCAGATAAACCGGCATTCATATCAAATGATATATAACAA
GGATATTTGAATCTGGCTGCGTTATGCTTCATCATAGGATATAATTCATG
TTTATCAGCCTCTGTTCCTTCATCTTTGATGTACAGTTGTTGTTGCAAAT
GTATTGAATATTTGTGCAGTTGCATTTATGGCGTTTAATCTATCCTTTTC
AATAGCGCCAACACTGCCTTGTTGTTCGAAATGTGTTTTCAAAAATAAGC
GAAGGTCTTGTGCAACTTTTATATTATCAGAAAAACGTTTTACTAATTCA
ATATCGTGCATATTTAATTTGATAGACTTCATATCTGCCTCAATATTTTT
AATTAATCCAGCCAAAACTTCAAAGTTTGTTTTTAATTTAAAAACATAGA
TAATGAGTTTTCTAACTAACATGTCTTGTTTCGACATGTTCGTTGTATCC
ATGTCATTCAATGCTTTAATCAATCGTAAGTCCGACTGGTAACGGACTTG
TCTATTCTTGTTTGAATAGTCTTTATCATGATCTACACAGAGTCTATCTA
TTGCATTAACTGGCAAGCTGGTAGTTTCTGGCATTCCGTTTTGGTCCAAT
CGCAAGTCGGTGCTTGTGCCTGGACCACAAAAGTTGTATCCTGGCACATG
CATCTCACCTTTATATTTAACAGACATCTAAAAAATCATAAATGTTTTCT
AACATAGTTACAGATACTTACTTTAATATTCACTATAACAATTGCAAAAA
AATTAAACAATATTTGGATAAACCGCACCAAAATTACTTGGAATATAATT
TAAACAATTTTTTTATAATCAGAAATCAAGTAGTAACTTGCGAGCCGGTT
TATTACCTAGCAAATTTATTTGAATCATATAAAAATCAATTAGACAAGTC
TCATCCTATTTTGATTGGGCAGCATGCGAATCAATTATTTGTCATCTTAG
ATAAAATAGATACTGTTTTATTGGAATTCGATCATACGACATTATGGAAA
CATTTTCAGAAAAATCCTGTGATCTCGCTGAACATACTGAACCTATCTCC
CCAGTTCAGAGAGCATGTCGGCGGAGCAGAAGCTCAAGTATTAATCGAGG
AAATAGAGATGCTTTATCTCATCGGTCAAAGGCCTCAAGTCGCAATGTTT
ACAATGAAGGAAGCTTAAACCAGAATAATACCAATGTGCATAAGGCATAT
ATGCCCAATATGCGAGCTAATATTTCGCGTCAATCAATATCTGAAGTCTC
TCGACCAAATGAACGGGAGTTATCGGCATCTGCTGAATTCATTACAGGAC
ACGATATTATTGATGGAGATAGTGAAATGTCTACAACAGACAATCTTCAC
ATGGAAGTTAATGAGGATGCTCAGCAACGCGATTACCCAGGAAGCCGAAG
ATTTAATTCCTTCCAGTTCAATCCATCTATGCGCAAGTATAGAGAACTGG
AGGGCCGCTTTCGGAGGTTTTCAAAGACACTGAGTTTACCCGCTTCGTGG
TTGGACAGGAAGAACAGGGTGATGATGGATTCCGGCATATGCAGATGTAT
GTCGAAGTTTATACTAACATGTCAGTTTATGCCTTTAGGGCTAAACTTAA
TGTCCTATTTGTTTCAGGGGTCGCTGGGCCTGACAGACCCCACGCAATCG
AATATTGCACAAAATCAAGATCAAGAATCGATAACGGAGTCAGAGTCAGA
ATCGAACAGCCCAGTAATTTCACAATAAAGAAAACTAATGATAAAGTAGA
CAATTTTTCTTTGGCGCTCAATGTAAAAACCATACAGGAAGCAGTTCTAA
TTTTGGAAAAATCAGAACCATTCCATTTCATAAAGAAAAAAACAATCATG
GAAAGTTTAATACAAAAATTTGGTATCAATACAAAGTCTAATTTTGTTAA
TTTCCTTGTCCCGAAAATTGATTTGTATGATTGGAAGTCCCATATCTTCG
TGGGTGAATCTGGAATTGGCAAAACAAACTTTGCCCTTAGTCATTTCAAA
AAACCAGTGCATGTAAAACATTTACAGATGCTTAATAGAGTGACATGTGA
TATGAAAAGTTGTGATGGCATAGTATTTGATGACTTATATTTTAATAAAT
TTCGTCTTGAAGAGTTCTTGTGTCTAGTGGATTGCGCATTTGAAAATGAC
GTTAACATCAAATACTCAGTAATTGATATTTATTAATTATTATTTTCCAG
TCTGATACAACTCTTGTATAAATTTGCCGAAACCAAGAACATCGTTTACT
AATCTGAGATGTGACAAATGCAACAGTTATTTGCGATTATACATTGGCAT
ATGTAAAACCAAC
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protein sequence of SMED30007492-orf-1

>SMED30007492-orf-1 ID=SMED30007492-orf-1|Name=SMED30007492-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
MFSNIVTDTYFNIHYNNCKKIKQYLDKPHQNYLEYNLNNFFIIRNQVVTC
EPVYYLANLFESYKNQLDKSHPILIGQHANQLFVILDKIDTVLLEFDHTT
LWKHFQKNPVISLNILNLSPQFREHVGGAEAQVLIEEIEMLYLIGQRPQV
AMFTMKEA*
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protein sequence of SMED30007492-orf-2

>SMED30007492-orf-2 ID=SMED30007492-orf-2|Name=SMED30007492-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=216bp
MQMYVEVYTNMSVYAFRAKLNVLFVSGVAGPDRPHAIEYCTKSRSRIDNG
VRVRIEQPSNFTIKKTNDKVDNFSLALNVKTIQEAVLILEKSEPFHFIKK
KTIMESLIQKFGINTKSNFVNFLVPKIDLYDWKSHIFVGESGIGKTNFAL
SHFKKPVHVKHLQMLNRVTCDMKSCDGIVFDDLYFNKFRLEEFLCLVDCA
FENDVNIKYSVIDIY*
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protein sequence of SMED30007492-orf-3

>SMED30007492-orf-3 ID=SMED30007492-orf-3|Name=SMED30007492-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=186bp
MSVKYKGEMHVPGYNFCGPGTSTDLRLDQNGMPETTSLPVNAIDRLCVDH
DKDYSNKNRQVRYQSDLRLIKALNDMDTTNMSKQDMLVRKLIIYVFKLKT
NFEVLAGLIKNIEADMKSIKLNMHDIELVKRFSDNIKVAQDLRLFLKTHF
EQQGSVGAIEKDRLNAINATAQIFNTFATTTVHQR*
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protein sequence of SMED30007492-orf-4

>SMED30007492-orf-4 ID=SMED30007492-orf-4|Name=SMED30007492-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=245bp
METFSEKSCDLAEHTEPISPVQRACRRSRSSSINRGNRDALSHRSKASSR
NVYNEGSLNQNNTNVHKAYMPNMRANISRQSISEVSRPNERELSASAEFI
TGHDIIDGDSEMSTTDNLHMEVNEDAQQRDYPGSRRFNSFQFNPSMRKYR
ELEGRFRRFSKTLSLPASWLDRKNRVMMDSGICRCMSKFILTCQFMPLGL
NLMSYLFQGSLGLTDPTQSNIAQNQDQESITESESESNSPVISQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006259DNA metabolic process
GO:0006260DNA replication
GO:0018142protein-DNA covalent cross-linking
Vocabulary: molecular function
TermDefinition
GO:0016779nucleotidyltransferase activity
GO:0016888endodeoxyribonuclease activity, producing 5'-phosphomonoesters
GO:0042624ATPase activity, uncoupled