SMED30007490
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007490 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 19
Alignments
SMED30007490 aligns in the following genomic locations:
Homology
BLAST of SMED30007490 vs. TrEMBL
Match: K1Q6I8 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10017507 PE=4 SV=1) HSP 1 Score: 83.1889 bits (204), Expect = 9.411e-13 Identity = 106/432 (24.54%), Postives = 180/432 (41.67%), Query Frame = 3 Query: 324 FKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKI----ELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNIN-------SHXXXXXXXXXXXXXXXXXASLLFQFTYIQ-TYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKK--NRLTNENCYKLSNQYVLTKSY--LFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYP 1571 + Q ++++ V V ++ + ++ D DW Y DV + GL +G V I L ++ G+VI+LFE+I G+ I + + IV+WF+ IP + I+ I +C E +S FQ+ + I LIR V V C ++SE+I+ S N H + R +G+T+ ++ + L+F FT +SG EF P L YF V I+ ++G L + + V + + + D + V + +D YL K + K I +C+ + + N ++ C K S +TK+Y +F +YVSP + G I Y + + + K + D S I+ F ++Y + Y GY M +N+ P Sbjct: 10 YAQMRNNRAVQIFVFLVMVGLNIADLTNDWLLYHDVDAAEEGLAFGPTEESVRIALLFFSITGAVIFLFEIIN-----FGKDIFSGNPWLDMDIASAIVIWFEDIPQITISLVILACREEPISVFQLGKASVVILGALIRLFVGIVWAC----RRRSEEIECIYSSKGKNKDNCKKQKHPVIRFFMFLGLTLLVIGSCLIFLFTQTTWKHSGFLEFNEPHSLFEGEYVDEHYFQRVGIYGNIGFLDTRNKSKQNTPLEDQWVRMFEIHDLRHDYKSIVIALEFSNDRSYLQVTK--IIPKYNKTI--DCFNIDRTEGINITSDPPCSKSS---TMTKTYKIIFKFEYVSPTNHLILGDIVYNVMGTV------HDKCEQGSVTEDPATGNSYIMVGNFTTALRYYQVKDDYK-----GNGY-MIPSNSSSRP 413 HSP 2 Score: 77.0258 bits (188), Expect = 6.854e-11 Identity = 98/434 (22.58%), Postives = 186/434 (42.86%), Query Frame = 3 Query: 363 VLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKI----ELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLN-----HYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYK---LSNQYVLTKSYLFYIKYV-SPDEYRQFGVIKYQSL-------------LAIYPEKYK----YKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYH-KNNMYSVRTIWKTGYAMCETNALQYP 1571 + ++T + LD DW F+ D+ ++PGLVYG + + +V ++G+ +FE+ IG+++ I + + +W + IP LII IA C E +VS FQI++ + ++ R + ++ Y + ID +S+ + L + I I I S+ + + + F PK + N RYF+ V+IF + + N H++ + S+Y + R + ++S + + Y+ H + K LK + EC+++ + +T + Y L N V S +F +K++ S R+FG I+Y L + E +K Y + + Y+ + G L ++T +FY ++ + T+W+TG++ C+ P Sbjct: 481 LFLMTFGLDFLDFLVDWLFFRDIHGIKPGLVYGPIESSIVYSSLFFAIVGTFAIVFEI-----ANIGKELFFEPAWINPDIVSCFFLWIEDIPQLIIGVRIAMCREEAVSLFQIIKASALLYGIVGRISLLSIRYF------SKKGIDALQSMRKGWKYALKFMFLSGMIFILIGSVSVFVLTLFEREANESIRFNHPKSMFEEDYNEARYFDNVSIFFNHPVFDLNMYNETQRVHFVKLLSIYEI----RSRKSALFKIS-YDELTKTKYVIWH-EDKNGQLKAD-----ECYRINPMRGTVTFQQYYCDHFLWNDQV--SSLIFKLKFIPSSIPERRFGDIQYNVLVRELGTCHSPQMDIVTTIENHKGEGHYDQAVIHYYRTRPGISQDHHLIVGGHSTARFYMFDKDLIDIATVWRTGFSFCKGTGNLAP 890
BLAST of SMED30007490 vs. TrEMBL
Match: A0A2C9L1I0 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106066829 PE=4 SV=1) HSP 1 Score: 75.8702 bits (185), Expect = 6.985e-11 Identity = 59/214 (27.57%), Postives = 102/214 (47.66%), Query Frame = 3 Query: 408 DWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKF-NSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQ-TYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNH 1043 DW FY+DV M G+VYG D V L +++G++ ++FE+I + I E + +SL +V+WF IP +II+ + C E +S FQ+ + I +++R +V+ + D++ KK HL +++ ++G+ + A +F + SG F+ P L + + RYF V+IF S H + N Sbjct: 24 DWLFYIDVKDMGTGIVYGEPDQDAVRALLAFSIIGTLAFIFEIIKLFLEFINEPDESWFYSDSLCALVIWFVDIPQMIISFSLILCREEPISVFQLGKACVIITGIVVRILVSLATFYNDKHNKK--------------HHLKFKVCFMLGMITEFSFAVTIFALNQTERNDSGTTSFRVPTTLLDDQFSDQRYFAHVSIFAS----HAHFFNQ 219
BLAST of SMED30007490 vs. TrEMBL
Match: A0A0B7ACU3 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF106638 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 2.286e-10 Identity = 87/424 (20.52%), Postives = 167/424 (39.39%), Query Frame = 3 Query: 396 DTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKG------------ENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIK--YVSPD---EYRQFG-----VIKYQSLLAIYPEKYKYK---------KIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPI 1574 D +DW F+ DVV GLVYG V L V +++G+ ++FE I + R + + L +V+W + +P +II+ + C E +S FQ+++ + + +++IR +VT+V+YC + K + + I+ + I + + F + +G ++ P + + + RYF V+IF H N+A+ Q M +RL+ S D F+ ++ G +N +EC+ + +T + S + S ++K + P + + FG V K Q+ + Y + Y+ ++ ++ + FY +++ V +WKTG+ CE++ PI Sbjct: 42 DFVSDWLFFTDVVIAESGLVYGPPEPVAVWFLLVFSIIGTFTFIFECINLWWEFF-RGNPWLDSDWLSAVVIWIEDVPQVIISLSLILCREEPISVFQLVKAIVIMISVVIRIVVTSVKYCNKKAIKSRHHVKIKAVIM------------LGIIVEAFAVGGIFFLTQTERNNAGDISYRVPSTVIEDGYDDQRYFRDVSIFF-----------HDRNLAN----QNPQTNSMVDWIRLATIYSIREGDQEASSFRVEYEQAGGSLNKLAISERDYDNWTLSECYSIDPNTKNITQVSNTICSQPGFFSNSTSLFMKLTFEKPGSIFKRKIFGDVHMNVKKLQNGVCTSVNDYADSIESSISNNIPLTFHYYRTNETLHPGNVKHLLQSGIPSFYTSSDIIDVSVVWKTGWKDCESSGHVAPI 437
BLAST of SMED30007490 vs. TrEMBL
Match: A0A0B6YUH2 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF35296 PE=4 SV=1) HSP 1 Score: 73.559 bits (179), Expect = 5.024e-10 Identity = 81/321 (25.23%), Postives = 146/321 (45.48%), Query Frame = 3 Query: 342 SQKVYRIV-LVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEM-IFIICLLIGRKIEL-IKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQT-YSGHFEFKAPKQLTANYINPGRYFNTVNIFL-SMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKN----RLTNENCYKLSNQ 1277 S + RI L++++ V+ LD +DW FYV + GLVYG + V L V +++GSV ++ E+ IFI L G + + + I++W + IP ++I+ IA C E +VS FQ+ + + ++I I T V +C + K K I + + ++GI + A+ +F T + +G F+ P + +++ RY V+ FL + + YL+ AS+ L +F L D +L FK + ++ G +IN W + ++ + + CY +++Q Sbjct: 2 SARCCRIFFLIVSLIVNALDFISDWLFYVSAEKVEKGLVYGPVDPHQVTALLVFSIVGSVGFVVEIAIFIFELRKGEDHHGDLLLDLVSAIIIWVEDIPQVVISFSIALCREEAVSIFQLTKAGLVLVGVIIHIIATIVRFCRNFESK--------KQICGVR------LCLMLGIFTEGAFAAAIFYLTQTEPDGNGQPSFRVPTTIMDEHLSDSRYLTDVSTFLHNSDLFDSWYLDTNKKDASINW--------------LRLFSLDRLKDENHLSFKLNYEHV-GIQMINMALWIATSLQSTSAIKWSLSECYSINHQ 293
BLAST of SMED30007490 vs. TrEMBL
Match: A0A2C9L714 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106067084 PE=4 SV=1) HSP 1 Score: 73.1738 bits (178), Expect = 5.538e-10 Identity = 57/208 (27.40%), Postives = 96/208 (46.15%), Query Frame = 3 Query: 396 DTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTY---SGHFEFKAPKQLTANYINPGRYFNTVNIFLS 1010 D +DW FY +V + G+VYG V L ++G++ ++ E++ + RK I + L +V+W + IP +II+ + C E +S FQ+++ I + IR +V+ V+YC ++D I SH + I + + A L F QT SG F+ P + + ++ RYF V+IF S Sbjct: 43 DFISDWLFYAEVKGIGKGIVYGEPDQVAVWALLAFCIIGTITFILEIVNLWWESF-RKNAWIDSDLLSAVVIWIEDIPQVIISFCLILCREEPISVFQLVKASVIITGIFIRILVSLVKYC-------NKDA--------IRSHHHVKFKVCIMLGMIFEAAFALAIFVLTQTERNGSGTVSFRVPTTIIDDQLDDQRYFGNVSIFAS 234
BLAST of SMED30007490 vs. Planmine SMEST
Match: SMESG000006820.1 (SMESG000006820.1) HSP 1 Score: 851.277 bits (2198), Expect = 0.000e+0 Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 3 Query: 297 MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL 1598 MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHLLYRILTIIGITIQIVLASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL Sbjct: 1 MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHLLYRILTIIGITIQIVLASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL 434 The following BLAST results are available for this feature:
BLAST of SMED30007490 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30007490 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30007490 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30007490 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30007490 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30007490 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007490 ID=SMED30007490|Name=SMED30007490|organism=Schmidtea mediterranea sexual|type=transcript|length=1720bpback to top protein sequence of SMED30007490-orf-1 >SMED30007490-orf-1 ID=SMED30007490-orf-1|Name=SMED30007490-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=435bp MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPback to top Annotated Terms
The following terms have been associated with this transcript:
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