SMED30007490

Overview
NameSMED30007490
Smed IDSMED30007490
Length (bp)1720
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007490 (SMED30007490) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007490 (SMED30007490) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007490 (SMED30007490) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007490 (SMED30007490) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007490

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cement glandSMED30007490h1SMcG0007941 GDAG01049680.1SmedSxl_ww_GDAG01.fsa_ntPMID:279364
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
head regionSMED30007490h1SMcG0007941 SmedASXL_014984SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30007490h1SMcG0007941 dd_Smed_v4_93926_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30007490h1SMcG0007941 dd_Smed_v4_93926_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007490h1SMcG0007941 dd_Smed_v4_93926_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007490h1SMcG0007941 Contig35162uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007490h1SMcG0007941 Contig35162newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30007490h1SMcG0007941 dd_Smed_v6_9227_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30007490h1SMcG0007941 Contig52632newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30007490h1SMcG0007941 Contig52632uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30007490h1SMcG0007941 dd_Smed_v4_9227_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007490 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.011229:2746..4466 -8588
Homology
BLAST of SMED30007490 vs. TrEMBL
Match: K1Q6I8 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10017507 PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.411e-13
Identity = 106/432 (24.54%), Postives = 180/432 (41.67%), Query Frame = 3
Query:  324 FKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKI----ELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNIN-------SHXXXXXXXXXXXXXXXXXASLLFQFTYIQ-TYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKK--NRLTNENCYKLSNQYVLTKSY--LFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYP 1571
            + Q ++++ V   V ++ + ++  D   DW  Y DV +   GL +G     V I L   ++ G+VI+LFE+I       G+ I      +  +    IV+WF+ IP + I+  I +C E  +S FQ+ +    I   LIR  V  V  C     ++SE+I+   S    N        H + R    +G+T+ ++ + L+F FT     +SG  EF  P  L         YF  V I+ ++G L  +  +          V   + + +  D +  V   +  +D  YL   K  +  K    I  +C+ +   +  N  ++  C K S    +TK+Y  +F  +YVSP  +   G I Y  +  +      + K      + D     S I+   F    ++Y   + Y        GY M  +N+   P
Sbjct:   10 YAQMRNNRAVQIFVFLVMVGLNIADLTNDWLLYHDVDAAEEGLAFGPTEESVRIALLFFSITGAVIFLFEIIN-----FGKDIFSGNPWLDMDIASAIVIWFEDIPQITISLVILACREEPISVFQLGKASVVILGALIRLFVGIVWAC----RRRSEEIECIYSSKGKNKDNCKKQKHPVIRFFMFLGLTLLVIGSCLIFLFTQTTWKHSGFLEFNEPHSLFEGEYVDEHYFQRVGIYGNIGFLDTRNKSKQNTPLEDQWVRMFEIHDLRHDYKSIVIALEFSNDRSYLQVTK--IIPKYNKTI--DCFNIDRTEGINITSDPPCSKSS---TMTKTYKIIFKFEYVSPTNHLILGDIVYNVMGTV------HDKCEQGSVTEDPATGNSYIMVGNFTTALRYYQVKDDYK-----GNGY-MIPSNSSSRP 413          

HSP 2 Score: 77.0258 bits (188), Expect = 6.854e-11
Identity = 98/434 (22.58%), Postives = 186/434 (42.86%), Query Frame = 3
Query:  363 VLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKI----ELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLN-----HYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYK---LSNQYVLTKSYLFYIKYV-SPDEYRQFGVIKYQSL-------------LAIYPEKYK----YKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYH-KNNMYSVRTIWKTGYAMCETNALQYP 1571
            + ++T  +  LD   DW F+ D+  ++PGLVYG + + +V       ++G+   +FE+       IG+++      I  + +    +W + IP LII   IA C E +VS FQI++    +  ++ R  + ++ Y         + ID  +S+     + L  +     I I I   S+     + +  +    F  PK +     N  RYF+ V+IF +  +      N     H++ + S+Y +    R   +   ++S +     + Y+  H + K   LK +     EC+++   +  +T +  Y    L N  V   S +F +K++ S    R+FG I+Y  L             +    E +K    Y +  + Y+ +  G      L    ++T +FY    ++  + T+W+TG++ C+      P
Sbjct:  481 LFLMTFGLDFLDFLVDWLFFRDIHGIKPGLVYGPIESSIVYSSLFFAIVGTFAIVFEI-----ANIGKELFFEPAWINPDIVSCFFLWIEDIPQLIIGVRIAMCREEAVSLFQIIKASALLYGIVGRISLLSIRYF------SKKGIDALQSMRKGWKYALKFMFLSGMIFILIGSVSVFVLTLFEREANESIRFNHPKSMFEEDYNEARYFDNVSIFFNHPVFDLNMYNETQRVHFVKLLSIYEI----RSRKSALFKIS-YDELTKTKYVIWH-EDKNGQLKAD-----ECYRINPMRGTVTFQQYYCDHFLWNDQV--SSLIFKLKFIPSSIPERRFGDIQYNVLVRELGTCHSPQMDIVTTIENHKGEGHYDQAVIHYYRTRPGISQDHHLIVGGHSTARFYMFDKDLIDIATVWRTGFSFCKGTGNLAP 890          
BLAST of SMED30007490 vs. TrEMBL
Match: A0A2C9L1I0 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106066829 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.985e-11
Identity = 59/214 (27.57%), Postives = 102/214 (47.66%), Query Frame = 3
Query:  408 DWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKF-NSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQ-TYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNH 1043
            DW FY+DV  M  G+VYG    D V  L   +++G++ ++FE+I +    I    E   + +SL  +V+WF  IP +II+  +  C E  +S FQ+ +    I  +++R +V+   +  D++ KK               HL +++  ++G+  +   A  +F     +   SG   F+ P  L  +  +  RYF  V+IF S    H  + N 
Sbjct:   24 DWLFYIDVKDMGTGIVYGEPDQDAVRALLAFSIIGTLAFIFEIIKLFLEFINEPDESWFYSDSLCALVIWFVDIPQMIISFSLILCREEPISVFQLGKACVIITGIVVRILVSLATFYNDKHNKK--------------HHLKFKVCFMLGMITEFSFAVTIFALNQTERNDSGTTSFRVPTTLLDDQFSDQRYFAHVSIFAS----HAHFFNQ 219          
BLAST of SMED30007490 vs. TrEMBL
Match: A0A0B7ACU3 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF106638 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.286e-10
Identity = 87/424 (20.52%), Postives = 167/424 (39.39%), Query Frame = 3
Query:  396 DTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKG------------ENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIK--YVSPD---EYRQFG-----VIKYQSLLAIYPEKYKYK---------KIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPI 1574
            D  +DW F+ DVV    GLVYG      V  L V +++G+  ++FE I +      R    +  + L  +V+W + +P +II+  +  C E  +S FQ+++ +  + +++IR +VT+V+YC  +  K    + +   I+            +  I     +  + F     +  +G   ++ P  +  +  +  RYF  V+IF            H  N+A+       Q   M   +RL+   S    D     F+ ++    G            +N   +EC+ +      +T  +    S     + S   ++K  +  P    + + FG     V K Q+ +      Y             +   Y+ ++      ++     +    FY  +++  V  +WKTG+  CE++    PI
Sbjct:   42 DFVSDWLFFTDVVIAESGLVYGPPEPVAVWFLLVFSIIGTFTFIFECINLWWEFF-RGNPWLDSDWLSAVVIWIEDVPQVIISLSLILCREEPISVFQLVKAIVIMISVVIRIVVTSVKYCNKKAIKSRHHVKIKAVIM------------LGIIVEAFAVGGIFFLTQTERNNAGDISYRVPSTVIEDGYDDQRYFRDVSIFF-----------HDRNLAN----QNPQTNSMVDWIRLATIYSIREGDQEASSFRVEYEQAGGSLNKLAISERDYDNWTLSECYSIDPNTKNITQVSNTICSQPGFFSNSTSLFMKLTFEKPGSIFKRKIFGDVHMNVKKLQNGVCTSVNDYADSIESSISNNIPLTFHYYRTNETLHPGNVKHLLQSGIPSFYTSSDIIDVSVVWKTGWKDCESSGHVAPI 437          
BLAST of SMED30007490 vs. TrEMBL
Match: A0A0B6YUH2 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF35296 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.024e-10
Identity = 81/321 (25.23%), Postives = 146/321 (45.48%), Query Frame = 3
Query:  342 SQKVYRIV-LVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEM-IFIICLLIGRKIEL-IKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQT-YSGHFEFKAPKQLTANYINPGRYFNTVNIFL-SMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKN----RLTNENCYKLSNQ 1277
            S +  RI  L++++ V+ LD  +DW FYV    +  GLVYG +    V  L V +++GSV ++ E+ IFI  L  G      +  + +  I++W + IP ++I+  IA C E +VS FQ+ +    +  ++I  I T V +C +   K        K I  +       +  ++GI  +   A+ +F  T  +   +G   F+ P  +   +++  RY   V+ FL +  +    YL+     AS+                L +F    L D  +L FK  + ++ G  +IN   W   + ++    + +   CY +++Q
Sbjct:    2 SARCCRIFFLIVSLIVNALDFISDWLFYVSAEKVEKGLVYGPVDPHQVTALLVFSIVGSVGFVVEIAIFIFELRKGEDHHGDLLLDLVSAIIIWVEDIPQVVISFSIALCREEAVSIFQLTKAGLVLVGVIIHIIATIVRFCRNFESK--------KQICGVR------LCLMLGIFTEGAFAAAIFYLTQTEPDGNGQPSFRVPTTIMDEHLSDSRYLTDVSTFLHNSDLFDSWYLDTNKKDASINW--------------LRLFSLDRLKDENHLSFKLNYEHV-GIQMINMALWIATSLQSTSAIKWSLSECYSINHQ 293          
BLAST of SMED30007490 vs. TrEMBL
Match: A0A2C9L714 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106067084 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.538e-10
Identity = 57/208 (27.40%), Postives = 96/208 (46.15%), Query Frame = 3
Query:  396 DTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTY---SGHFEFKAPKQLTANYINPGRYFNTVNIFLS 1010
            D  +DW FY +V  +  G+VYG      V  L    ++G++ ++ E++ +      RK   I  + L  +V+W + IP +II+  +  C E  +S FQ+++    I  + IR +V+ V+YC       ++D         I SH   +    I + +    A  L  F   QT    SG   F+ P  +  + ++  RYF  V+IF S
Sbjct:   43 DFISDWLFYAEVKGIGKGIVYGEPDQVAVWALLAFCIIGTITFILEIVNLWWESF-RKNAWIDSDLLSAVVIWIEDIPQVIISFCLILCREEPISVFQLVKASVIITGIFIRILVSLVKYC-------NKDA--------IRSHHHVKFKVCIMLGMIFEAAFALAIFVLTQTERNGSGTVSFRVPTTIIDDQLDDQRYFGNVSIFAS 234          
BLAST of SMED30007490 vs. Planmine SMEST
Match: SMESG000006820.1 (SMESG000006820.1)

HSP 1 Score: 851.277 bits (2198), Expect = 0.000e+0
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 3
Query:  297 MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHXXXXXXXXXXXXXXXXXASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL 1598
            MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHLLYRILTIIGITIQIVLASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL
Sbjct:    1 MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRPGLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSLMIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTAVEYCLDQNEKKSEDIDVAKSILNINSHLLYRILTIIGITIQIVLASLLFQFTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHYMNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGENVINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFGVIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHKNNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL 434          
The following BLAST results are available for this feature:
BLAST of SMED30007490 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1Q6I89.411e-1324.54Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A2C9L1I06.985e-1127.57Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A0B7ACU32.286e-1020.52Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A0B6YUH25.024e-1025.23Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A2C9L7145.538e-1027.40Uncharacterized protein OS=Biomphalaria glabrata O... [more]
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BLAST of SMED30007490 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007490 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000006820.10.000e+0100.00SMESG000006820.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007490 ID=SMED30007490|Name=SMED30007490|organism=Schmidtea mediterranea sexual|type=transcript|length=1720bp
TCTCACTATGGTTATTGTGAGAAACCAGTAAGCACTTTCGAAACAAATTC
CAAAAATGCAAGTTTTTTTGAAATGGTTAAGCAGTCTTTTACACAAATAC
AAATTATTATTATTCTATTTAATATTCGGCATTTTCATTTCATAGTTTGT
ATAGTACTTTATGTATATTTTCTTATTATTTACCAGATATGAACTAGATT
CATTTTAACGAATGAGTTATTTCTGCCAGCATTGTCAAATTTTAGAAATA
TTTAGAGTTAACGAAACAGGAAAAATATTCATTATTTCCATTTGGAATGA
ATTGTGGAGGAATTATTGATTGGTTCAAGCAATGGAAGCATTCTCAAAAG
GTCTATCGAATAGTCCTAGTCCTCACTATCTACGTATCGACTTTAGATAC
ATACACAGACTGGGGATTTTACGTTGATGTCGTATCAATGAGGCCAGGCC
TTGTCTATGGATACTTAACAAATGATGTGGTAATTCCATTATTTGTTTGC
ACTGTTCTTGGCTCTGTTATCTATTTATTTGAAATGATTTTCATAATATG
TCTGTTAATAGGCCGAAAAATAGAACTGATAAAGTTTAATTCTTTAATGA
TAATTGTCATGTGGTTTGATGTCATTCCTCATTTGATAATCAACTCTTTT
ATTGCGTCTTGTCATGAATATTCAGTTTCGTATTTCCAAATATTAAGAAC
TGTGACATCAATTGGAGCAATGCTGATAAGATTTATTGTAACAGCAGTCG
AATATTGCCTGGATCAAAATGAAAAGAAATCGGAAGATATTGATGTAGCC
AAATCGATTCTAAACATTAACAGCCATCTATTATACAGAATACTGACTAT
CATTGGCATAACGATTCAAATTGTTCTGGCGTCTTTACTATTTCAATTTA
CATATATTCAAACATATTCCGGCCATTTTGAATTCAAAGCCCCGAAGCAA
CTGACTGCAAATTACATTAACCCAGGTAGATATTTTAATACTGTCAATAT
ATTTTTATCTATGGGAATTCTACATCGAAAATACTTAAATCATTACATGA
ACGTTGCATCCTTGTACCACGTAATGTACTCTCAAAGATATGGAATGACT
TGCGATGTTAGATTAAGCGTTTTTCAATCTAAAAACTTATCTGATTATTT
GTATCTTCATTTTAAAAAGAAATTTGTAAATCTGAAAGGCGAAAATGTTA
TTAATAATGAATGTTGGAAGTTATACACGAAGAAAAATCGGTTAACCAAT
GAAAATTGTTATAAATTATCCAATCAATATGTACTAACTAAATCTTATCT
ATTCTACATAAAGTATGTCAGTCCTGATGAATATCGACAATTTGGAGTGA
TCAAATACCAATCGCTGTTGGCAATTTATCCAGAAAAATATAAATACAAA
AAAATTCGTCTAAAATATTTTTCTTCCGATAACGGTGGATTTCTATCGAG
CATTTTAGAAAAAAGATTTAATAATACTTTCAAATTTTACCACAAAAACA
ATATGTATTCGGTTAGGACAATTTGGAAAACTGGATATGCAATGTGTGAA
ACGAACGCCCTCCAATACCCGATTTATGTAAGAAGCAAGTTTACGCTTTG
AATAAAAAAAAACGTTTAGAAACTAACTTTAGTGTATTACGGAGTTTTGG
TTGTAGTAAAATTTCTAATTATCAACAATATTAAAAGGTGAGACAATAAA
AGAAAAAAAGTATTATTATC
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protein sequence of SMED30007490-orf-1

>SMED30007490-orf-1 ID=SMED30007490-orf-1|Name=SMED30007490-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=435bp
MNCGGIIDWFKQWKHSQKVYRIVLVLTIYVSTLDTYTDWGFYVDVVSMRP
GLVYGYLTNDVVIPLFVCTVLGSVIYLFEMIFIICLLIGRKIELIKFNSL
MIIVMWFDVIPHLIINSFIASCHEYSVSYFQILRTVTSIGAMLIRFIVTA
VEYCLDQNEKKSEDIDVAKSILNINSHLLYRILTIIGITIQIVLASLLFQ
FTYIQTYSGHFEFKAPKQLTANYINPGRYFNTVNIFLSMGILHRKYLNHY
MNVASLYHVMYSQRYGMTCDVRLSVFQSKNLSDYLYLHFKKKFVNLKGEN
VINNECWKLYTKKNRLTNENCYKLSNQYVLTKSYLFYIKYVSPDEYRQFG
VIKYQSLLAIYPEKYKYKKIRLKYFSSDNGGFLSSILEKRFNNTFKFYHK
NNMYSVRTIWKTGYAMCETNALQYPIYVRSKFTL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000224cement gland
PLANA:0002032epidermal cell
PLANA:0002089reproductive organ