Peptidase S1 domain-containing protein

Overview
NamePeptidase S1 domain-containing protein
Smed IDSMED30007489
Length (bp)927
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Peptidase S1 domain-containing protein (SMED30007489) t-SNE clustered cells

Violin plots show distribution of expression levels for Peptidase S1 domain-containing protein (SMED30007489) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007489

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30007489h1SMcG0015733 SmedASXL_003884SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30007489h1SMcG0015733 SmedASXL_003884SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30007489h1SMcG0015733 dd_Smed_v6_3728_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30007489h1SMcG0015733 OX_Smed_1.0.16093ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: KLK13 (kallikrein related peptidase 13 [Source:HGNC Symbol;Acc:HGNC:6361])

HSP 1 Score: 105.531 bits (262), Expect = 5.080e-26
Identity = 74/228 (32.46%), Postives = 113/228 (49.56%), Query Frame = 1
Query:  163 HQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYT-SEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            H  P   ++ +    +CGG L++   VLT AHC+ +              +++LG++ L  +   E+  +V   I HP Y  S     + +D+ L++    ++ T Y  I+   L  N    PG  C V GWG+   P  N  + LQ A++ + S+E+C +  Y  K  D+ +CAGT      +C+GD GGPL C          GIVS+G  PCGQP  P V+TR+S
Sbjct:   45 HSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE------------GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLTGY--IQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEEC-RQVYPGKITDNMLCAGTKEGGKDSCEGDSGGPLVCN-----RTLYGIVSWGDFPCGQPDRPGVYTRVS 252          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: CTRL (chymotrypsin like [Source:HGNC Symbol;Acc:HGNC:2524])

HSP 1 Score: 93.5893 bits (231), Expect = 1.183e-21
Identity = 78/244 (31.97%), Postives = 118/244 (48.36%), Query Frame = 1
Query:  115 AISTALKTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            A+S + + +NG   ++  +P  VS++     H CGG+L++ + V+T AHC       N    +++   + LGEYD        +   V++ I HPS+ S       NDV L+K     + T        +   N     G  C   GWG L   G  T   LQ+ +L +++   C + ++     DS ICAG   + AS+C+GD GGPL C  G N  V +GIVS+G   C     P V+TR+S
Sbjct:   27 ALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHC-------NVSPGRHF---VVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTT---MNNDVTLLKLASPAQYTT-RISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQC-RQYWGSSITDSMICAG--GAGASSCQGDSGGPLVCQKG-NTWVLIGIVSWGTKNC-NVRAPAVYTRVS 251          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: GZMM (granzyme M [Source:HGNC Symbol;Acc:HGNC:4712])

HSP 1 Score: 92.4337 bits (228), Expect = 2.735e-21
Identity = 73/255 (28.63%), Postives = 117/255 (45.88%), Query Frame = 1
Query:   94 FVVLSFCAISTA----LKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKT-FYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
             +VL+  A+S       + I G + I H  P M S++   +H+CGG L++   VLT AHC+           +  + R+ LG   LH +      F +   I HP Y    +    ND+AL++ DG ++ +        +     +   G RC + GWG L   G   +R+L+E  L V+    C+ + F+N     S +C    + D + CKGD GGPL C  G+   V   ++S+    C   + P V T ++
Sbjct:    8 LLVLALGALSVGSSFGTQIIGGREVIPHSRPYMASLQRNGSHLCGGVLVHPKWVLTAAHCLAQ---------RMAQLRLVLG---LHTLDSPGLTFHIKAAIQHPRYKPVPALE--NDLALLQLDGKVKPS-RTIRPLALPSKRQVVAAGTRCSMAGWG-LTHQGGRLSRVLRELDLQVLDTRMCNNSRFWNGSLSPSMVCLAADSKDQAPCKGDSGGPLVCGKGR---VLARVLSFSSRVCTDIFKPPVATAVA 243          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: CELA2A (chymotrypsin like elastase 2A [Source:HGNC Symbol;Acc:HGNC:24609])

HSP 1 Score: 90.1225 bits (222), Expect = 1.924e-20
Identity = 79/244 (32.38%), Postives = 117/244 (47.95%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN----HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGD-RCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKT-FYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYG-HVPCGQPYIPIVFTRIS 843
            + + G +   + +P  VS++   N    H CGG+L+  + VLT AHCI   R           +R+ LG ++L+          V+KI++H  + S      GND+AL+K    +  TD   I+   L   G   P +  C V GWG L   G     +LQ+  L+V+    CS + ++      S ICAG G+   S+C GD GGPL C     +    GIVS+G  + C   + P VFTR+S
Sbjct:   28 RVVGGEEARPNSWPWQVSLQYSSNGKWYHTCGGSLIANSWVLTAAHCISSSR----------TYRVGLGRHNLYVAESGSLAVSVSKIVVHKDWNSNQ-ISKGNDIALLKLANPVSLTDK--IQLACLPPAGTILPNNYPCYVTGWGRLQTNGA-VPDVLQQGRLLVVDYATCSSSAWWGSSVKTSMICAG-GDGVISSCNGDSGGPLNCQASDGRWQVHGIVSFGSRLGCNYYHKPSVFTRVS 256          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: KLK8 (kallikrein related peptidase 8 [Source:HGNC Symbol;Acc:HGNC:6369])

HSP 1 Score: 88.1965 bits (217), Expect = 8.544e-20
Identity = 79/264 (29.92%), Postives = 122/264 (46.21%), Query Frame = 1
Query:   64 KMSQKVVFHLFVVLSFCAISTAL--KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDI---NGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            + ++  +F L +  ++   S A   K + G +   H  P   ++      +CGG L+ GN VLT AHC            K  K+ + LG++ L    G E++  V + I HP Y S     + +D+ L++        D   +   V  I   +  ++PG +C V GWG++  P EN    L  A + +   + C    Y  +  D  +CAG+ +  A  C+GD GGPL C DG  Q    GI S+G  PCG+   P V+T I
Sbjct:    7 RAAKTWMFLLLLGGAWAGHSRAQEDKVLGGHECQPHSQPWQAALFQGQQLLCGGVLVGGNWVLTAAHC------------KKPKYTVRLGDHSLQNKDGPEQEIPVVQSIPHPCYNSSDVEDHNHDLMLLQL------RDQASLGSKVKPISLADHCTQPGQKCTVSGWGTVTSPRENFPDTLNCAEVKIFPQKKCEDA-YPGQITDGMVCAGS-SKGADTCQGDSGGPLVC-DGALQ----GITSWGSDPCGRSDKPGVYTNI 245          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: svh-1 (HGF/MSP/plasminogen-like protein [Source:UniProtKB/TrEMBL;Acc:Q17800])

HSP 1 Score: 65.0846 bits (157), Expect = 2.186e-11
Identity = 67/272 (24.63%), Postives = 120/272 (44.12%), Query Frame = 1
Query:   61 SKMSQKVVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSI--KLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYN-------VLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDC--SKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            S  SQ  + ++ V     A S   + + G + +   FP   ++  K    H CG ++L+   ++T AHC  +         +   + + +G++D +   G E+ F + +I  +P Y       + +D+A+++         Y  I++N       +   + +  PG +C V GWGS+   G   A  LQ A + +++  DC  S   Y+     S  CAG       +C+GD GGP  C       V  G++S+G   C Q   P ++T ++
Sbjct:  688 SSASQCGLRYVEVNARDAAKSRIARVVGGFETVPGAFPWTAALRNKATKAHHCGASILDKTHLITAAHCFEEDE-------RVSSYEVVVGDWDNNQTDGNEQIFYLQRIHFYPLYKD----IFSHDIAILEIP-------YPGIEFNEYAQPICLPSKDFVYTPGRQCVVSGWGSM---GLRYAERLQAALIPIINRFDCVNSSQIYS-SMSRSAFCAGYLEGGIDSCQGDSGGPFACRREDGAFVLAGVISWGD-GCAQKKQPGIYTMVA 936          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: try-1 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q23528])

HSP 1 Score: 63.1586 bits (152), Expect = 3.058e-11
Identity = 64/244 (26.23%), Postives = 114/244 (46.72%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSI-KLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSK--TFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQV-GIVSYGHVPCGQPYIPIVFTRISTVA 852
            + I GS+   H +P  V +   + +H CGG+L++ N VLT AHC    R       +   + + +G +      G     +VT + +HP Y   + F    D A+++    + NT        +  +  +      C V GWGS +     +A  L+E  + ++S   CS    +    ++ S +CAG       +C+GD GGPL C   ++ H ++ G+VS+G + C +P +P V+  + + +
Sbjct:   57 RLIGGSESSPHSWPWTVQLLSRLGHHRCGGSLIDPNFVLTAAHCFAKDR-------RPTSYSVRVGGHR----SGSGSPHRVTAVSIHPWY--NIGFPSSYDFAIMRIHPPV-NTSTTARPICLPSLPAVEN--RLCVVTGWGSTIEGSSLSAPTLREIHVPLLSTLFCSSLPNYIGRIHLPSMLCAGYSYGKIDSCQGDSGGPLMCA--RDGHWELTGVVSWG-IGCARPGMPGVYGNVHSAS 281          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 55.0694 bits (131), Expect = 1.666e-8
Identity = 51/177 (28.81%), Postives = 79/177 (44.63%), Query Frame = 1
Query:  358 EKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLL------PPGENTARLLQEASLIVMSNEDCSKTF-----YNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPC----GQPYIPIVFTRIS 843
            E+ F V +  +H  Y ++ +    ND+AL++    +       +   V D + + K      V+G+G  L       P    ++ LQ  S+ ++S++DC KT+      +VK     ICAG          GD GGPL       ++VQ+GI SYG         Q   P V+TRIS
Sbjct:  102 ERSFSVKEAYIHSGYNNQTA---DNDIALLRISSDLSKLGIKPVCL-VHDDSKLLKQYKNGVVIGYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGA--YLHGTAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRIS 272          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 54.6842 bits (130), Expect = 1.722e-8
Identity = 51/177 (28.81%), Postives = 79/177 (44.63%), Query Frame = 1
Query:  358 EKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLL------PPGENTARLLQEASLIVMSNEDCSKTF-----YNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPC----GQPYIPIVFTRIS 843
            E+ F V +  +H  Y ++ +    ND+AL++    +       +   V D + + K      V+G+G  L       P    ++ LQ  S+ ++S++DC KT+      +VK     ICAG          GD GGPL       ++VQ+GI SYG         Q   P V+TRIS
Sbjct:   52 ERSFSVKEAYIHSGYNNQTA---DNDIALLRISSDLSKLGIKPVCL-VHDDSKLLKQYKNGVVIGYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGA--YLHGTAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRIS 222          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: tpr (gene:FBgn0034661 transcript:FBtr0071743)

HSP 1 Score: 100.523 bits (249), Expect = 7.412e-24
Identity = 74/248 (29.84%), Postives = 123/248 (49.60%), Query Frame = 1
Query:  109 FCAISTALKTINGSQEI-MHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNV-LDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPL-FCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
             C I+   K I G QE  +HQ+P +  +       C  +LLN   +LT +HC++         F+  +  + L E+D    +  + D +V ++I HP Y +     Y ND+A++K D   E  +++ + + V +   G S  G+   V GWG+L   G  T+  LQE  + ++S ++C K+ Y  K  D+ +C G       +C+GD GGPL     G  +H   G+VS+G   C +   P V+ R++
Sbjct:  117 VCGIANIQKRIVGGQETEVHQYPWVAMLLYGGRFYCAASLLNDQFLLTASHCVYG--------FRKERISVRLLEHDRKMSHMQKIDRKVAEVITHPKYNAR---NYDNDIAIIKLD---EPVEFNEVLHPVCMPTPGRSFKGENGIVTGWGAL-KVGGPTSDTLQEVQVPILSQDECRKSRYGNKITDNMLCGGYDEGGKDSCQGDSGGPLHIVASGTREHQIAGVVSWGE-GCAKAGYPGVYARVN 348          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: CG18735 (gene:FBgn0042098 transcript:FBtr0071744)

HSP 1 Score: 87.4261 bits (215), Expect = 3.456e-19
Identity = 70/254 (27.56%), Postives = 126/254 (49.61%), Query Frame = 1
Query:  118 ISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP-------GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFY-NVKYIDSNICAG---TGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            I+T  + + G +  +H++P M+ +    N  CG +L+N    LT AHC+      N  Y +    R+   E++    +    D +V+++++HP Y++     + +D+AL++F+        + ++  + D++ +  P       G    V GWG+ L  G   +  LQE  + ++S E+C  + Y   K  D+ ICAG    G  D  +C+GD GGP+      + +   GIVS+G   C +P  P V+TR+ +
Sbjct:   77 INTRHRIVGGQETEVHEYPWMIMLMWFGNFYCGASLVNDQYALTAAHCV------NGFYHRLITVRLL--EHNRQDSHVKIVDRRVSRVLIHPKYSTR---NFDSDIALIRFN--------EPVRLGI-DMHPVCMPTPSENYAGQTAVVTGWGA-LSEGGPISDTLQEVEVPILSQEECRNSNYGESKITDNMICAGYVEQGGKD--SCQGDSGGPMHVLGSGDAYQLAGIVSWGEG-CAKPNAPGVYTRVGS 306          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: CG7432 (gene:FBgn0038727 transcript:FBtr0301213)

HSP 1 Score: 85.5001 bits (210), Expect = 3.914e-18
Identity = 68/204 (33.33%), Postives = 101/204 (49.51%), Query Frame = 1
Query:  253 AHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKD--FQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISK---PGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSN-ICAGTGNSDASACKGDLGGPLFCTDGKNQH-VQVGIVSYGHVPCGQPYIPIVFTRIS 843
            AHC  D R      F   +F + LG+ DL           F V ++  H  + S + FY  ND+A++  D  +  + Y         I    K   PG R  VVGWG+    G+ +    Q A L +  NEDC ++++  + I+ N ICAG  +    AC+GD GGPL      + H VQ+G+VS+G+  CG+P  P V+TR++
Sbjct:  518 AHCTRDSR---QKPFAARQFTVRLGDIDLSTDAEPSDPVTFAVKEVRTHERF-SRIGFY--NDIAILVLDKPVRKSKYVIPVCLPKGIRMPPKERLPGRRATVVGWGTTYYGGKESTSQRQ-AELPIWRNEDCDRSYF--QPINENFICAGYSDGGVDACQGDSGGPLMMR--YDSHWVQLGVVSFGNK-CGEPGYPGVYTRVT 709          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: CG9372 (gene:FBgn0036891 transcript:FBtr0074945)

HSP 1 Score: 81.6481 bits (200), Expect = 4.526e-17
Identity = 65/206 (31.55%), Postives = 100/206 (48.54%), Query Frame = 1
Query:  253 AHCIWDLRYWNNDYFKYYKFRIF--LGEYDLHYMYGIE-KDFQVTKIIMHPSYTSEMSFYYGNDVALVK------FDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYI-DSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            AHCI          +K  K  IF  LGEY+ H +     +DF++  +++H  Y  +    Y ND+A+V+      F+ YI       +  +  D N I        V GWG+    G ++  +L E +L V    DC  +F  V+++ D+ +CAG       +C+GD GGPL       + V +GIVS+G V CGQ   P ++TR+
Sbjct:  215 AHCI----------YKKNKEDIFVRLGEYNTHMLNETRARDFRIANMVLHIDYNPQ---NYDNDIAIVRIDRATIFNTYIWPVCMPPVNEDWSDRNAI--------VTGWGTQKFGGPHS-NILMEVNLPVWKQSDCRSSF--VQHVPDTAMCAGFPEGGQDSCQGDSGGPLLVQLPNQRWVTIGIVSWG-VGCGQRGRPGIYTRV 395          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: lambdaTry (gene:FBgn0043470 transcript:FBtr0088121)

HSP 1 Score: 79.337 bits (194), Expect = 9.300e-17
Identity = 68/245 (27.76%), Postives = 112/245 (45.71%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCE------VVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            + + G    + Q+P  +S++   NH CGGT+   N +++ AHC+  L    N         I  G  ++ +  G +++ +V +II+HP Y +  + Y   D A++  DG  E  D          +  I    +R +      V GWG+    G   + +LQE S+ V+ N +C K  Y++      +CAG       AC+GD GGPL        +  +GIVS+G   C +   P V+  +  V
Sbjct:   35 RIVGGQDTNITQYPHQISMRYRGNHRCGGTIYRSNQIISAAHCVNTLSGPEN-------LTIVAGSSNIWFPTGPQQELEVREIIIHPKYRTLNNDY---DAAILILDGDFEFNDA---------VQPIELAKERPDHDTPVTVTGWGTT-SEGGTISDVLQEVSVNVVDNSNC-KNAYSIMLTSRMLCAGVNGGGKDACQGDSGGPLVY-----NNTLLGIVSWG-TGCAREKYPGVYCSVPDV 252          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:112285 (zgc:112285 [Source:ZFIN;Acc:ZDB-GENE-050913-132])

HSP 1 Score: 103.605 bits (257), Expect = 3.245e-25
Identity = 87/278 (31.29%), Postives = 135/278 (48.56%), Query Frame = 1
Query:   61 SKMSQKVVFHLFVV----LSFCAISTALKTINGSQEIMHQFPQMVSIKLMDN------HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS-KPGDRCEVVGWGSLLPPGENT--ARLLQEASLIVMSNEDC-SKTFYNVKYIDSNICAGTGNSDA--SACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            S++ Q  + HL       L+    +T  + ++G++   H +P  VS+++         H+CGGTL++ N VLT AHC     +          +RI LG++ L      E+ F V +I  H  +          D+ALVK    I+ +++  I+Y  L    I+  PG  C V GWG      EN   A  L +A L ++  + C  K F+  +  DS ICAG  +++   +AC+GD GGPL C  G+++    GIVS+G + C     P VFTR + 
Sbjct:   30 SRLQQHKILHLDWPKDCGLAHFKPNTVERIVSGNEARPHSWPWQVSLQVRPRGSKHYVHVCGGTLIHKNWVLTAAHC-----FQKGKAEDASSWRIVLGKHQLKRSETAERFFPVKRIYRHEHFRYPAHSELDYDIALVKAATDIQPSNF--IRYACLPRKQINLNPGHYCWVTGWGDTRGGKENVSLAEALNQARLPIIDYKTCRQKKFWGDRVRDSMICAGFRDTEGTPAACQGDSGGPLLCQVGRDRWEVHGIVSFGPIGCTVENKPSVFTRTAA 300          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch73-182e20.4 (si:ch73-182e20.4 [Source:ZFIN;Acc:ZDB-GENE-131127-155])

HSP 1 Score: 93.9745 bits (232), Expect = 5.843e-22
Identity = 85/252 (33.73%), Postives = 119/252 (47.22%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVL-DINGISKPGDRCEVVGWGSLLPPGENTAR-------------LLQEASLIVMSNEDCSKTFYNVKYIDSN-ICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            + + G       +P MVS++   NHICGG+L+N   VLT AHC+                 ++LG++  +     E    V+ II HPSY S     Y ND+AL++    +  +  DYIK   L D      PG R    GWG +   G+   R             +LQE  L V SN DC+   +    I+ N ICAGT +   +   GD GGPL  +   +  VQ G+VS+G+  C QP +P VF R+S
Sbjct:   15 RIVGGLNSTEGAWPWMVSLRYYGNHICGGSLINNEWVLTAAHCV---------NLTRSNMLVYLGKWRRYAADVNEITRTVSNIIPHPSYNSTT---YDNDIALLQLSSTVHYS--DYIKPVCLADEQSNFPPGTRSWATGWGRIGVSGKGGIRGRTTVSVPLPPPGILQEVKLKVYSNADCNSICHG--RINPNMICAGTRSGGKATFSGDSGGPLV-SKQCSVWVQAGVVSHGYG-CAQPNLPEVFIRVS 248          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch73-182e20.4 (si:ch73-182e20.4 [Source:ZFIN;Acc:ZDB-GENE-131127-155])

HSP 1 Score: 89.3521 bits (220), Expect = 1.862e-20
Identity = 83/237 (35.02%), Postives = 114/237 (48.10%), Query Frame = 1
Query:  178 MVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVL-DINGISKPGDRCEVVGWGSLLPPGENTAR-------------LLQEASLIVMSNEDCSKTFYNVKYIDSN-ICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            MVS++   NHICGG+L+N   VLT AHC+                 ++LG++  +     E    V+ II HPSY S     Y ND+AL++    +  +DY  IK   L D      PG R    GWG +   G+   R             +LQE  L V SN DC+   +    I+ N ICAGT +   +   GD GGPL  +   +  VQ G+VS+G+  C QP +P VF R+S
Sbjct:    1 MVSLRYYGNHICGGSLINNEWVLTAAHCV---------NLTRSNMLVYLGKWRRYAADVNEITRTVSNIIPHPSYNSTT---YDNDIALLQLSSTVHYSDY--IKPVCLADEQSNFPPGTRSWATGWGRIGVSGKGGIRGRTTVSVPLPPPGILQEVKLKVYSNADCNSICHG--RINPNMICAGTRSGGKATFSGDSGGPLV-SKQCSVWVQAGVVSHGY-GCAQPNLPEVFIRVS 219          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: ctrl (chymotrypsin-like [Source:ZFIN;Acc:ZDB-GENE-040912-147])

HSP 1 Score: 88.9669 bits (219), Expect = 3.657e-20
Identity = 76/239 (31.80%), Postives = 114/239 (47.70%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP-GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            + +NG   +   +P  VS++  +  H CGG+L+N   V+T AHC     Y       +Y   + LGE+D        +   + K I HP Y S+    + ND+ L+K     + T    I    L  +  S P G RC   GWG       ++ R+LQ+ +L ++S   C + +   +  D+ ICAG   S  S+C+GD GGPL C +      QVGIVS+G   C     P V+ R+S
Sbjct:   31 RIVNGENAVSGSWPWQVSLQQSNGFHFCGGSLINQYWVVTAAHCRVQAGY-------HY---VILGEHDRGSSAESVQVKSIAKAITHPYYNSQN---FNNDITLLKLSSPAQLTSR--ISPVCLAASSTSIPSGTRCVTTGWGKT--GSTSSPRILQQTALPLLSPAQCKQYWGQNRITDAMICAGA--SGVSSCQGDSGGPLVC-ESSGAWYQVGIVSWGTSDC-NVRTPAVYARVS 248          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:55888 (zgc:55888 [Source:ZFIN;Acc:ZDB-GENE-040426-834])

HSP 1 Score: 88.9669 bits (219), Expect = 2.753e-19
Identity = 74/240 (30.83%), Postives = 113/240 (47.08%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYM----YGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            + I G +   H +P  VS++  D   CGG +L+   V+T  HC          + +Y K  ++     LH +        +  QV KI  H +Y  + +    ND+AL+K    +    +      +   N    P   C V GWGS+   G   +RL QE ++ V   + C++ FY  K + S ICAG       AC+GD GGPL C DG+ ++   G+VS+G V CG+   P V+T +
Sbjct:   56 RIIGGKEAWAHSWPWQVSLQYNDVPTCGGAILDQLWVITAGHC----------FKRYKKPSMWNAVVGLHNLDNANESSRESIQVQKIFSHKNYNQKTN---ENDIALLKLQSPLV---FSKFVRPIGVFNNDLPPLVTCTVTGWGSVTENGPQASRL-QEVNVTVYEPQKCNR-FYRGKVLKSMICAGANEGGMDACQGDSGGPLSCFDGE-RYKLAGVVSWG-VGCGRAQKPGVYTTL 275          

HSP 2 Score: 59.6918 bits (143), Expect = 1.683e-9
Identity = 65/246 (26.42%), Postives = 104/246 (42.28%), Query Frame = 1
Query:  127 ALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSY---TSEMSFYYGNDVALVK---FDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            A K  N S+   + +P   S++  D H C G L++   VL   HC+           K     + LG +DL++M G   D +  + + H       S++S  Y    A +    F   I + D + +       NG S     C   GWG      +    +L  A +  +S + C   + +     S++C  T  + +++C GD G PL C      H+ VG+ ++G   C QP  P VFTR+S 
Sbjct:  324 AFKVENVSEACPNAWPWQASLQNDDTHYCSGVLVHPRWVLAPRHCL----------VKAGDV-VVLGAHDLNFMSGQTVDVESVQSLSHNGRNRTVSDLSMIYLTVPARIGPLIFPVCITDKDDELV-------NGDSS---SCVTTGWGPRKATLDLQPEILHMARVKPLSEDTCRTGWGDGFNRQSHLC--THAAASTSCLGDSGAPLVCAKNGIYHL-VGLTTWGSKKC-QPQKPAVFTRVSA 544          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: noxa1 (NADPH oxidase activator 1 [Source:Xenbase;Acc:XB-GENE-6257646])

HSP 1 Score: 98.9821 bits (245), Expect = 1.691e-23
Identity = 86/274 (31.39%), Postives = 135/274 (49.27%), Query Frame = 1
Query:   85 FHLFVVLSF---CAIS-----TALKTINGSQEIMHQFPQMVSIKLMDN------HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS-KPGDRCEVVGWGSLLPPGENT--ARLLQEASLIVMSNEDCS-KTFYNVKYIDSNICAGTGN--SDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            F+LF+ L +   C +S     TA + ++G++   + +P  VS+++         H+CGGTL++ + +LT AHC     +          +RI +G+++L+     EK + V +I  H  ++         D+ALVK    I  T +  I Y  L    ++  PG  C V GWG       N   + +L +A L ++  + C  K F+  +  +S ICAG  N     +AC+GD GGPL C DG+ +    GIVS+G V C     P VFT+ ST
Sbjct:   12 FYLFIFLDWPGDCGVSFFQQNTAGRIVSGNEVRPYSWPWQVSLQVRPRGGKKYVHVCGGTLIHKSWILTAAHC-----FQKGKAEDAANWRIVVGKHNLNRTEATEKVYSVKRIYRHERFSYPQLNDLDYDIALVKPAEDIITTHF--IHYACLPKKEMAMHPGHFCWVTGWGDTRGGQGNVTLSEVLNQARLPIIDTKTCRHKKFWGDRIRESMICAGFRNVGGPPAACQGDSGGPLVCQDGRGRWEVHGIVSFGPVGCTVENKPSVFTKTST 278          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: noxa1 (NADPH oxidase activator 1 [Source:Xenbase;Acc:XB-GENE-6257646])

HSP 1 Score: 97.0561 bits (240), Expect = 1.149e-22
Identity = 82/260 (31.54%), Postives = 129/260 (49.62%), Query Frame = 1
Query:  103 LSFCAISTALKTINGSQEIMHQFPQMVSIKLMDN------HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS-KPGDRCEVVGWGSLLPPGENT--ARLLQEASLIVMSNEDCS-KTFYNVKYIDSNICAGTGN--SDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            +SF   +TA + ++G++   + +P  VS+++         H+CGGTL++ + +LT AHC     +          +RI +G+++L+     EK + V +I  H  ++         D+ALVK    I  T +  I Y  L    ++  PG  C V GWG       N   + +L +A L ++  + C  K F+  +  +S ICAG  N     +AC+GD GGPL C DG+ +    GIVS+G V C     P VFT+ ST
Sbjct:   40 VSFFQQNTAGRIVSGNEVRPYSWPWQVSLQVRPRGGKKYVHVCGGTLIHKSWILTAAHC-----FQKGKAEDAANWRIVVGKHNLNRTEATEKVYSVKRIYRHERFSYPQLNDLDYDIALVKPAEDIITTHF--IHYACLPKKEMAMHPGHFCWVTGWGDTRGGQGNVTLSEVLNQARLPIIDTKTCRHKKFWGDRIRESMICAGFRNVGGPPAACQGDSGGPLVCQDGRGRWEVHGIVSFGPVGCTVENKPSVFTKTST 292          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000015681.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:2721982:2727059:1 gene:ENSXETG00000009946.1 transcript:ENSXETT00000015681.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 96.2857 bits (238), Expect = 2.009e-22
Identity = 82/256 (32.03%), Postives = 127/256 (49.61%), Query Frame = 1
Query:  112 CAISTALKTINGSQEI-MHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP-GDRCEVVGWGSLLPPGE-NTARLLQEASLIVMSNEDCSKTFYNVKYIDSN--------ICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            C     L  I G Q+     +P  V I      +CGG+L+  N V++ AHC        N       + ++LG Y +  + G E    + + I+HP+YT   +   G+D+ALV+  G I  T++  I+   L   G++ P G +C V GWG++   G+      LQE ++ ++ N+ C+  F     ID          +CAG  +    +C+GD GGPL C +  N+   VGIVS+G V CGQP  P V+TR++ 
Sbjct:   19 CGRRQLLSRIMGGQDSKAGMWPWQVMIYSEAFELCGGSLITNNWVVSAAHCF-------NRTKPPSFYTVYLGVYQISVLNGNEVAMPIKQFIVHPNYTVPEN---GSDIALVELSGDIIYTNH--IQPVCLPTGGVNLPTGLQCWVTGWGNIASNGDLPEPNTLQEVAVPLIGNQQCNALFQVPSPIDPKTYVISNDMLCAGFIDGGKDSCQGDSGGPLVCAE-ANRWYLVGIVSFG-VGCGQPNRPGVYTRLNA 260          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: ctrl (chymotrypsin-like [Source:NCBI gene;Acc:548358])

HSP 1 Score: 93.2041 bits (230), Expect = 1.214e-21
Identity = 72/240 (30.00%), Postives = 112/240 (46.67%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            + +NG   +   +P  VS++     H CGG++++   V+T AHC                 R+ LGEYD        +   + K+  HP+Y S   F   ND+ L+K       ++       V   +     G+RC   GWG +      T   LQ+ +L ++SN +C + ++  K +++ +CAG   S AS+C GD GGPL C       V  GIVS+G   C  P  P V+ R+ST+
Sbjct:   33 RIVNGENAVPGSWPWQVSLQDSTGFHFCGGSVISDFWVVTAAHC-----------GVTTAHRVILGEYDRSSPAEPIQTKTIAKVFRHPNYNS---FTIANDITLLKLSSPASFSNI-VAPVCVASSSDAFNGGERCVTTGWGYVDAASRLTPNKLQQVALPLLSNTECQR-YWGSKILNTMVCAGA--SGASSCMGDSGGPLVCQR-NGAWVLAGIVSWGSSTC-SPSSPGVYARVSTL 252          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: TMPRSS9 (transmembrane serine protease 9 [Source:NCBI gene;Acc:100489279])

HSP 1 Score: 96.6709 bits (239), Expect = 1.537e-21
Identity = 79/242 (32.64%), Postives = 123/242 (50.83%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLM-DNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYG-IEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVL-DINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            K + GS  +  ++P  VS+ L    H CG  L++   +L+ AHC        + Y     +  +LG   L+ + G +EK F++ K   HP Y     +   NDVAL++    +  T+   I+   L DI+ I   G RC + GWGS    G   +R LQ+AS+ ++ ++ C K FY ++     +CAG       +C GD GGPL C +   +    GI S+G+  C +PY P V+TRI++V
Sbjct:  861 KIVGGSGSVRGEWPWQVSLWLRRKEHKCGAVLISDRWLLSAAHCF-------DIYSDPKLWAAYLGTPFLNGVEGRVEKIFRIHK---HPFYNV---YTLDNDVALLELPSPLTYTNL--IRPICLPDISHIFPEGTRCFITGWGST-KEGGAMSRQLQKASVSIVGDQTCKK-FYPIQISPRMLCAGFMQGGVDSCSGDAGGPLACREPSGRWFLAGITSWGYG-CARPYFPGVYTRITSV 1084          

HSP 2 Score: 89.3521 bits (220), Expect = 3.787e-19
Identity = 65/245 (26.53%), Postives = 111/245 (45.31%), Query Frame = 1
Query:  115 AISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            A+    + + GS     +FP  VS++  + H CG T++    +++ AHC        ND+     +  ++    L           +  II HPSY  + + Y   DVA+++ D  ++   Y      + D   +   G +C + GWG L         +LQ+A++ +M    C+  + NV   +  +CAG       +C+GD GGPL C +   +    GIVS+G V C +   P V+ R+S +
Sbjct:  231 AMQKTNRIVGGSDATKGEFPWQVSLRENNEHFCGATVIGDKWLVSAAHCF-------NDFQDPAVWVAYIATTSLSGTDSSTVKATIRNIIKHPSYDPDTADY---DVAVLELDSPLKFNKYTQ-PVCLPDPTHVFPVGKKCIITGWGYLKEDNLVKPEVLQKATVAIMDQSLCNSLYSNV-VTERMLCAGYLEGKIDSCQGDSGGPLVCEEPSGKFFLAGIVSWG-VGCAEARRPGVYVRVSKI 462          

HSP 3 Score: 75.0998 bits (183), Expect = 2.106e-14
Identity = 61/240 (25.42%), Postives = 110/240 (45.83%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP-GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            K + G   +  + P   S+K    H CG T++    +++ AHC         +  K  +    +G   L     I     + ++I HP + + ++  +  DVA+++    +  T   Y++   L       P G +C + GWG++     +   +LQ+AS+ ++  + CS   YN    +  ICAG  +    +C+GD GGPL C +        GIVS+G + C Q   P V++R++ +
Sbjct:  530 KIVGGLDAVRGEIPWQASLKEGSRHFCGATIIGDRWLVSAAHCF--------NQTKVDQVTAHMGSTALSGADTIAIKISLKRVIQHPHF-NPLTLDF--DVAVLELASSL--TFNKYVQPVCLPSALQKFPAGWKCMISGWGNIKEGNVSKPEVLQKASVGIIDQKICS-VLYNFSITERMICAGFLDGKVDSCQGDSGGPLACEESPGIFFLAGIVSWG-IGCAQAKKPGVYSRVTKL 754          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Gzmm (granzyme M (lymphocyte met-ase 1) [Source:MGI Symbol;Acc:MGI:99549])

HSP 1 Score: 101.679 bits (252), Expect = 6.831e-25
Identity = 76/240 (31.67%), Postives = 116/240 (48.33%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP--GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKT-FYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            + I G + + H  P M S++   +H+CGG L++   VLT AHC+ +              ++ LG ++LH +      F + + I HP Y  +    Y ND+AL+K D  ++ +    +K   L     SKP  G  C   GWG +   G   AR LQE  L V+  + C+ + F+N   IDS +C   G+   + CKGD GGPL C  G+      GI+S+    C   + P V T ++
Sbjct:   26 QIIGGREAVPHSRPYMASLQKAKSHVCGGVLVHRKWVLTAAHCLSE---------PLQNLKLVLGLHNLHDLQDPGLTFYIREAIKHPGYNHK----YENDLALLKLDRRVQPSKN--VKPLALPRKPRSKPAEGTWCSTAGWG-MTHQGGPRARALQELDLRVLDTQMCNNSRFWNGVLIDSMLCLKAGSKSQAPCKGDSGGPLVCGKGQVD----GILSFSSKTCTDIFKPPVATAVA 245          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Klk8 (kallikrein related-peptidase 8 [Source:MGI Symbol;Acc:MGI:1343327])

HSP 1 Score: 100.138 bits (248), Expect = 2.425e-24
Identity = 80/258 (31.01%), Postives = 121/258 (46.90%), Query Frame = 1
Query:   73 QKVVFHLFVVLSFCAISTAL--KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            Q  +  L  + ++  ++ A   K + G + I H  P   ++   +  ICGG L+    VLT AHC            K  K+ + LG++ L      E++ QV + I HP Y +     + +D+ L++          D +K   L  N   K G +C + GWG++  P EN    L  A + + S   C +  Y  K  +  +CAG+ N  A  C+GD GGPL C DG  Q    GI S+G  PCG+P  P V+T+I
Sbjct:   10 QPWILLLLFMGAWAGLTRAQGSKILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHC------------KKQKYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQNSANLG--DKVKPVQL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERA-YPGKITEGMVCAGSSNG-ADTCQGDSGGPLVC-DGMLQ----GITSWGSDPCGKPEKPGVYTKI 245          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Klk13 (kallikrein related-peptidase 13 [Source:MGI Symbol;Acc:MGI:3615275])

HSP 1 Score: 94.3597 bits (233), Expect = 4.234e-22
Identity = 69/230 (30.00%), Postives = 114/230 (49.57%), Query Frame = 1
Query:  157 IMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSY-TSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            + H  P   ++ +    +CGG L++   VLT AHC  D             + + LG++ L  +   E+  +V + I HP Y  +     + +D+ L++    ++ + +  ++   L  +     G  C V GWG+   P  N  + LQ A++ + S+E+C +  Y  K   + +CAGT      +C+GD GGPL C +GK      GI+S+G  PCGQP  P V+TR+S
Sbjct:   44 LPHSQPWQAALLIRGRLLCGGVLVHPKWVLTAAHCRKD------------GYTVHLGKHALGRVENGEQAMEVVRSIPHPEYQVTPTHLNHDHDIMLLELKSPVQLSSH--VRTLKLSADDCLPTGTCCRVSGWGTTTSPQVNYPKTLQCANIELRSDEEC-RQVYPGKITANMLCAGTKEGGKDSCEGDSGGPLIC-NGK----LYGIISWGDFPCGQPNRPGVYTRVS 253          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Klk1b9 (kallikrein 1-related peptidase b9 [Source:MGI Symbol;Acc:MGI:95293])

HSP 1 Score: 93.9745 bits (232), Expect = 5.229e-22
Identity = 84/262 (32.06%), Postives = 121/262 (46.18%), Query Frame = 1
Query:   91 LFVVLSFCAISTAL----KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEM--------SFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            LF+ LS   I  A     + + G +   +  P  V++   + +ICGG LL+ N VLT AHC             Y + ++ LG+ +L+      +   V+K  +HP Y   +         + Y ND+ L++     + TD   +K   L      K G  C   GWGS  P     A+ LQ  +L ++ NEDC K     K  D  +CAG  +     CKGD GGPL C DG    V  GI S+G  PCG+P  P V+T++
Sbjct:    6 LFLALSLGGIDAAPPVHSRIVGGFKCEKNSQPWHVAVYRYNEYICGGVLLDANWVLTAAHCY------------YEENKVSLGKNNLYEEEPSAQHRLVSKSFLHPGYNRSLHRNHIRHPEYDYSNDLMLLRLSKPADITDV--VKPIALPTEE-PKLGSTCLASGWGSTTPFKFQNAKDLQCVNLKLLPNEDCGKAHIE-KVTDVMLCAGETDGGKDTCKGDSGGPLIC-DG----VLQGITSWGFTPCGEPKKPGVYTKL 246          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Tmprss9 (transmembrane protease, serine 9 [Source:MGI Symbol;Acc:MGI:3612246])

HSP 1 Score: 96.2857 bits (238), Expect = 1.602e-21
Identity = 79/243 (32.51%), Postives = 123/243 (50.62%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMD-NHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYG-IEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP--GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            + + GS   + ++P  VS+ L    H CG  L+    +L+ AHC +D+      Y    ++  FLG   L    G +E+   V +I  HP Y      Y   DVAL++  G +  +         + + G ++P  G RC + GWGSL   G + AR LQ+A++ V+S + C + FY V+     +CAG       +C GD GGPL C +   Q V  G+ S+G+  CG+P+ P V+TR++ V
Sbjct:  867 RIVGGSAASLGEWPWQVSLWLRRREHRCGAVLVAERWLLSAAHC-FDI------YGDPMQWAAFLGTPFLSSTEGQLER---VARIYRHPFYNIYTLDY---DVALLELAGPVRRSRL----VRPICLPGPARPPDGARCVITGWGSL-REGGSMARQLQKAAVRVLSEQTCRR-FYPVQISSRMLCAGFPQGGVDSCSGDAGGPLACREPSGQWVLTGVTSWGY-GCGRPHFPGVYTRVAAV 1089          

HSP 2 Score: 76.6406 bits (187), Expect = 4.822e-15
Identity = 62/249 (24.90%), Postives = 113/249 (45.38%), Query Frame = 1
Query:  115 AISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVK------FDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            A  +A + + G +    +FP  VS++    H CG T++    +++ AHC        N++    ++    G   L          +V +I  HP+Y ++ + +   DVA+++      F  Y++        +       +  PG +C + GWG L         +LQ+A++ ++    CS + Y     D  +CAG  +    +C+GD GGPL C +   +    GIVS+G + C +   P V+TR++
Sbjct:  232 AWRSAGRIVGGVEAAPGEFPWQVSLRENHEHFCGATIIGARWLVSAAHCF-------NEFQDPAQWAAQAGSVHLSGSEASAVRTRVLRIAKHPAYDADTADF---DVAVLELARPLPFGRYVQPACLPAATH-------VFPPGKKCLISGWGYLKEDFLVKPEVLQKATVELLDQSLCS-SLYGHSLTDRMVCAGYLDGKVDSCQGDSGGPLVCEEPSGRFFLAGIVSWG-IGCAEARRPGVYTRVT 461          

HSP 3 Score: 75.485 bits (184), Expect = 1.211e-14
Identity = 65/251 (25.90%), Postives = 109/251 (43.43%), Query Frame = 1
Query:  115 AISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVK------FDGYIENTDYDYI--KYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            A+    + + G   +  + P   S+K    H CG T++    +L+ AHC         ++ K  + +  LG   L  + G      + ++ +HP Y   +  +   DVAL++      F+ YI+         K+ V         G +C + GWG++         +LQ+AS+ ++  + C    YN    D  +CAG       +C+GD GGPL C +        GIVS+G + C Q   P V+ RI+
Sbjct:  533 AMDKPTRIVGGISAVSGEVPWQASLKEGPRHFCGATVVGDRWLLSAAHCF--------NHTKVEQVQAHLGTVSLLGVGGSPVKLGLRRVALHPRYNPGILDF---DVALLELAQPLVFNKYIQPVCLPLAIHKFPV---------GRKCMISGWGNMQEGNATKPDILQKASVGIIEQKMCG-ALYNFSLTDRMLCAGFLEGRVDSCQGDSGGPLACEETPGVFYLAGIVSWG-IGCAQAKKPGVYARIT 761          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9UKR3|KLK13_HUMAN (Kallikrein-13 OS=Homo sapiens OX=9606 GN=KLK13 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 2.440e-25
Identity = 74/228 (32.46%), Postives = 113/228 (49.56%), Query Frame = 1
Query:  163 HQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYT-SEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            H  P   ++ +    +CGG L++   VLT AHC+ +              +++LG++ L  +   E+  +V   I HP Y  S     + +D+ L++    ++ T Y  I+   L  N    PG  C V GWG+   P  N  + LQ A++ + S+E+C +  Y  K  D+ +CAGT      +C+GD GGPL C          GIVS+G  PCGQP  P V+TR+S
Sbjct:   45 HSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE------------GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLTGY--IQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEEC-RQVYPGKITDNMLCAGTKEGGKDSCEGDSGGPLVCN-----RTLYGIVSWGDFPCGQPDRPGVYTRVS 252          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q61955|KLK8_MOUSE (Kallikrein-8 OS=Mus musculus OX=10090 GN=Klk8 PE=1 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.696e-23
Identity = 80/258 (31.01%), Postives = 121/258 (46.90%), Query Frame = 1
Query:   73 QKVVFHLFVVLSFCAISTAL--KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            Q  +  L  + ++  ++ A   K + G + I H  P   ++   +  ICGG L+    VLT AHC            K  K+ + LG++ L      E++ QV + I HP Y +     + +D+ L++          D +K   L  N   K G +C + GWG++  P EN    L  A + + S   C +  Y  K  +  +CAG+ N  A  C+GD GGPL C DG  Q    GI S+G  PCG+P  P V+T+I
Sbjct:   10 QPWILLLLFMGAWAGLTRAQGSKILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHC------------KKQKYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQNSANLG--DKVKPVQL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERA-YPGKITEGMVCAGSSNG-ADTCQGDSGGPLVC-DGMLQ----GITSWGSDPCGKPEKPGVYTKI 245          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q03238|GRAM_RAT (Granzyme M (Fragment) OS=Rattus norvegicus OX=10116 GN=Gzmm PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.043e-22
Identity = 75/238 (31.51%), Postives = 112/238 (47.06%), Query Frame = 1
Query:  139 INGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP--GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKT-FYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            I G + + H  P MVS++   +H+CGG L++   VLT AHC+ +            + ++  G + LH        F + + I HP Y    +  Y ND+AL+K DG ++ +    +K   L      KP  G RC   GWG     G+  A+ LQE  L ++    C+ + F+N    DS +C   G    + CKGD GGPL C  GK      GI+S+    C   + P V T ++
Sbjct:   22 IGGREAVPHSRPYMVSLQNTKSHMCGGVLVHQKWVLTAAHCLSE---------PLQQLKLVFGLHSLHDPQDPGLTFYIKQAIKHPGY----NLKYENDLALLKLDGRVKPSKN--VKPLALPRKPRDKPAEGSRCSTAGWGITHQRGQ-LAKSLQELDLRLLDTRMCNNSRFWNGVLTDSMLCLKAGAKGQAPCKGDSGGPLVCGKGKVD----GILSFSSKNCTDIFKPTVATAVA 239          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|P00774|CEL2A_RAT (Chymotrypsin-like elastase family member 2A OS=Rattus norvegicus OX=10116 GN=Cela2a PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.361e-22
Identity = 80/244 (32.79%), Postives = 119/244 (48.77%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN----HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGD-RCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSN-ICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGH-VPCGQPYIPIVFTRIS 843
            + + G +   + +P  VS++ + +    H CGG+L+  N VLT AHCI + R           +R+ LG + L          QV+K+++H  + ++     GND+ALVK    +  T    I+   L   G   P +  C V GWG L   G  T  +LQ+  L+V+    CS   +    + +N +CAG G+   S+C GD GGPL C     Q    GIVS+G  + C  P  P VFTR+S
Sbjct:   30 RVVGGQEASPNSWPWQVSLQYLSSGKWHHTCGGSLVANNWVLTAAHCISNSR----------TYRVLLGRHSLSTSESGSLAVQVSKLVVHEKWNAQ-KLSNGNDIALVKLASPVALTSK--IQTACLPPAGTILPNNYPCYVTGWGRLQTNGA-TPDVLQQGRLLVVDYATCSSASWWGSSVKTNMVCAG-GDGVTSSCNGDSGGPLNCQASNGQWQVHGIVSFGSTLGCNYPRKPSVFTRVS 258          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|O88780|KLK8_RAT (Kallikrein-8 OS=Rattus norvegicus OX=10116 GN=Klk8 PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.894e-22
Identity = 73/255 (28.63%), Postives = 119/255 (46.67%), Query Frame = 1
Query:   79 VVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDI-NGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRI 840
            ++F L    +    +   K + G +   H  P   ++   +  +CGG L+    VLT AHC            K  K+ + LG++ L      E++ QV + I HP + S     + +D+ L++    ++N+     K   +++ N   K G +C + GWG++  P EN    L  A + + S   C +  Y  K  +  +CAG+ N  A  C+GD GGPL C       V  GI ++G  PCG+P  P V+T+I
Sbjct:   14 LLFLLMGAWAGLTRAQGSKILEGQECKPHSQPWQTALFQGERLVCGGVLVGDRWVLTAAHC------------KKDKYSVRLGDHSLQKRDEPEQEIQVARSIQHPCFNSSNPEDHSHDIMLIR----LQNSANLGDKVKPIELANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERA-YPGKITEGMVCAGSSNG-ADTCQGDSGGPLVCNG-----VLQGITTWGSDPCGKPEKPGVYTKI 245          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: H2DRI7 (Trypsin-like serine protease OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 3.139e-87
Identity = 120/194 (61.86%), Postives = 152/194 (78.35%), Query Frame = 1
Query:  271 LRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 852
            ++ WN + FKYY+  ++L EYDL+ MYG+EK FQVTK I+HPSY  E ++  GNDVAL+KFDG+I N  YDYI YN LD+N I KPGDRCEVVGWGS+LPPG++ +R LQ+A++ ++SN +C + F NV  + SNICAG+G    S+C+GD GGPLFCTDGK  H+QVGIVSYG VPCGQP IP V+TR+S VA
Sbjct:    1 MKSWNLNTFKYYQMEVYLAEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGFISNGQYDYIHYNELDVNAIFKPGDRCEVVGWGSMLPPGQDISRFLQKAAINIISNSECGRIFSNVADVRSNICAGSGRLSTSSCRGDSGGPLFCTDGKRHHIQVGIVSYGTVPCGQPNIPSVYTRVSEVA 194          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A1I8JAW5 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007344g1 PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 1.796e-34
Identity = 98/262 (37.40%), Postives = 144/262 (54.96%), Query Frame = 1
Query:   91 LFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGI-EKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDY----IKYNVLDINGISKP--GDRCEVVGWGSLLPPGE--NTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCT-DGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            LF  LS       L  I+G +    Q P +VS++   NH CGGTL +  TV+T AHC     Y N+  F      +  GE DL        + ++V+++I+HP Y S   +  G+D+A++K      ++D D     IK    +  G+  P  G  C+V GWG++ P G   +T +++   S I  +N DC++ F  V      +CAG G++  +AC+GD GGPL+CT +GK +  QVGIVSYG VPCGQ  IP V+TR++T
Sbjct:    6 LFACLSVANAYDRL-IIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCF----YGNSGTFG---LAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILKL-----SSDADLGTATIKAGDFEDAGVDIPPEGSSCKVAGWGAIDPSGRYAHTRKMIGGVSSI--TNNDCARVFNRVLGNTKQVCAG-GDTTTNACRGDSGGPLYCTYNGKTR--QVGIVSYGRVPCGQVGIPTVYTRVAT 249          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A1I8GT16 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010631g1 PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.341e-34
Identity = 98/262 (37.40%), Postives = 143/262 (54.58%), Query Frame = 1
Query:   91 LFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGI-EKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDY----IKYNVLDINGISKP--GDRCEVVGWGSLLPPGE--NTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCT-DGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            LF  LS       L  I+G +    Q P +VS++   NH CGGTL +  TV+T AHC     Y N+  F      +  GE DL        + ++V+++I+HP Y S   +  G+D+A++K      ++D D     IK    +  G+  P  G  C+V GWG++ P G   +T +++   S I  +N DC++ F  V      +CAG G +  +AC+GD GGPL+CT +GK +  QVGIVSYG VPCGQ  IP V+TR++T
Sbjct:    6 LFACLSVANAYDRL-IIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCF----YGNSGTFG---LAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILKL-----SSDADLGTATIKAGDFEDAGVDIPPAGSSCKVAGWGAIDPSGRYAHTRKMIGGVSSI--TNNDCARVFNRVLGNTKQVCAG-GGTTTNACRGDSGGPLYCTYNGKTR--QVGIVSYGRVPCGQVGIPTVYTRVAT 249          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: U5EP40 (Putative trypsin-like serine protease OS=Corethrella appendiculata OX=1370023 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.418e-25
Identity = 83/259 (32.05%), Postives = 128/259 (49.42%), Query Frame = 1
Query:  103 LSFCAISTALKTINGSQEIMHQFPQMVSIKLMDN----------HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKF-DGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSL-LPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            LS  ++    K + GS    +Q+P  +S++   N          H CGG+++N N +LT AHC    +  N   F + +  +  GE+ L +    E+  QV + I+H SY   +     ND+A++K  + ++ N +   I +   D    S P     + GWGS+ +    N   +LQ   L ++  E+CS  +   +   SN+CAG  N   S CKGD GGPL  TDG    VQ+G+VS+G  PCG    P +F R+S
Sbjct:   21 LSLYSLEADHKIVGGSNAEPNQYPYQISLQWNFNETVANPRRPFHFCGGSVINANWILTAAHC----KVANLSEFGFIE--VVAGEHHLAFNDTTEQRRQVDRFILHESYGGGVG---PNDIAVIKVNESFVLNENVTRINFPERD----SIPSGNATLTGWGSMSITERPNAPNILQTVRLPIIEYEECSNLWNTTQLAASNVCAGELNGSKSGCKGDSGGPLVQTDGDLNVVQIGVVSWGSFPCGAEDKPGIFVRVS 266          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A0L8HIV6 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22013567mg PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.297e-25
Identity = 80/251 (31.87%), Postives = 131/251 (52.19%), Query Frame = 1
Query:   97 VVLSFCAISTALKTINGSQEIMHQFPQMVSIKLM--DNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            + ++ C ++T    + G      ++P MVS++L     H+CGGTL++   V+T AHC++      N + K   +R  LGE+ L    G E   ++  I+ HP++ +  +  Y ND+AL++ D   +   +   +  + D N   +PGD C  +GWG     G+ +  +LQE  + V SN +CS  +   + ++S+IC   GN D  AC GD GGPL C      ++ VGI S+G   C     P V+TR++
Sbjct:  146 IPIAECGVATTHMIVGGIPSKEGKWPWMVSLRLTWAKRHVCGGTLIHPRWVITAAHCVF-----GNQFEKESDWRAILGEFQLGVNTGKEMHRKIDLILRHPNFINGGN--YPNDIALMRLDHPADVAGFYVRQACIPDKNAGFRPGDECWTMGWGETKDYGDQS--ILQELQVHVRSNSECSIRWGRRRILNSHICV--GNGDNGACNGDSGGPLVCVRNGYYYL-VGITSWGVSGCRTSGYPSVYTRVA 384          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000043830.1 (elastase-1-like [Source:NCBI gene;Acc:103036513])

HSP 1 Score: 103.605 bits (257), Expect = 1.355e-25
Identity = 82/218 (37.61%), Postives = 120/218 (55.05%), Query Frame = 1
Query:  205 HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDC-SKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFC-TDGKNQ-HVQVGIVSYGHVP-CGQPYIPIVFTRIST 846
            HICGG+L++   V+T AHCI D++       ++Y  R+ LG+YDL+   G E+   V++IIMHP +T ++    GND+AL+K    +    Y  I  N    +     G  C + GWG LL  G  T  +LQEA + V+ +  C S  +++     + +CAG G+   S C+GD GGPL C TDG  + H   G+VS+G    C     P VFTR+S+
Sbjct:   62 HICGGSLISSLYVMTAAHCILDMQ------LRFY--RVVLGDYDLYKYEGGEQPRSVSRIIMHPGWTGDL--VNGNDIALLKLSSPVYGNSYTEIA-NFPYPDQTLPNGFTCYITGWG-LLATGGTTPDILQEAPIPVVEHSVCASPGWWSTAVKKTMVCAG-GDGKTSGCQGDSGGPLNCYTDGAWRVH---GVVSFGPASNCNIYTKPTVFTRVSS 263          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000039451.1 (trypsin-like [Source:NCBI gene;Acc:111192060])

HSP 1 Score: 94.3597 bits (233), Expect = 5.915e-22
Identity = 77/243 (31.69%), Postives = 116/243 (47.74%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS-KPGDRCEVVGWGSL-----LPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            K + G+  +   +P  VSI+ + +H CGG+L+N N +L+ AHC     + ++        R+ LG+  L           V +II HPSY S     + ND+AL++    +  T  DYI+   L   G +   G    V GWG +     LP  +N    LQE  L ++SN  C+  + +     + +CAG       +C+GD GGPL          Q GIVS+G+  C  P IP V+ R+S
Sbjct:   36 KIVGGNTAVSGSWPWQVSIQSLGSHFCGGSLINNNWILSAAHC-----FQSSQTSTLAFLRVSLGKQSLQTSELNSLTITVAQIINHPSYVSST---HDNDIALLQLSSSV--TFSDYIQPVCLAAAGSTLGSGTLVWVTGWGDISSDVSLPDPQN----LQEVELPIVSNTACTSAYGSGSITSNMMCAGLSQGGKDSCQGDSGGPLVIKQSTG-WTQAGIVSFGY-GCALPKIPGVYARVS 262          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000048530.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:20283923:20290642:1 gene:ENSAMXG00000040955.1 transcript:ENSAMXT00000048530.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.2781 bits (225), Expect = 4.337e-21
Identity = 66/242 (27.27%), Postives = 119/242 (49.17%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENT--ARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 852
            + + G+  +  ++P   S+++   HICGG L+    V++ AHC +D R ++   +      ++LG+  L      E+  +++ I +H  Y  E   Y   D+AL++ D  +               + + +PG  C V GWG++   GE    + +LQ+  + ++S E C ++ Y        +CAG  +    AC+GD GGPL C +   +    G+VS+G   CG+P    V+TRI+ ++
Sbjct:   28 RVVGGADAVEGEWPWQASLQVSGRHICGGALIASQWVVSAAHCFYDDRLYSPSVWT-----VYLGKLRLRGTSQTEEALRLSHIHIHQYYDDENHDY---DLALLRLDRPVSAGTLAQPACLPSHTHQL-EPGLLCWVTGWGAMKEGGEKRGPSNVLQKVDVRLVSEEACVRS-YGYIITPRMLCAGYRSGGKDACQGDSGGPLVCQEPSGRWFLAGVVSWGR-GCGRPDYYGVYTRITRLS 258          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: zgc:112160 (chymotrypsin B-like [Source:NCBI gene;Acc:103028134])

HSP 1 Score: 89.3521 bits (220), Expect = 1.860e-20
Identity = 78/240 (32.50%), Postives = 115/240 (47.92%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIK-LMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKF--DGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            + +NG + + H +P   S++     H CGG+L+N N V+T AHC  ++R            RI LGE++        +  +V K+  HP++  +      ND+ALVK      IE+T        +   N +  PG +C   GWG L      T   LQ+ +L ++SN+DC K  +      + ICAG     AS+C GD GGPL C    N    VGIVS+G   C     P V+ R++
Sbjct:   33 RIVNGEEAVPHSWPWQASLQDFTGFHFCGGSLINENWVVTAAHC--NVRT---------SHRIILGEHNKAKSGEKIQTLRVNKVFTHPNWNPKT---IENDIALVKLASPASIESTVSPVC---LAGSNDVFAPGLKCVTSGWGLLRHNALFTPDKLQQTALPLLSNDDC-KNHWGSNITGTMICAGA--DGASSCMGDSGGPLVCQK-DNVWTLVGIVSWGSSTCST-TTPAVYARVT 250          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ctrb1 (chymotrypsin A-like [Source:NCBI gene;Acc:103027485])

HSP 1 Score: 88.5817 bits (218), Expect = 3.338e-20
Identity = 79/246 (32.11%), Postives = 115/246 (46.75%), Query Frame = 1
Query:  118 ISTALKTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIE-NTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            IS   + +NG + + H +P  VS++     H CGG+L+N   V+T AHC  ++R            R+ LGE+D        +   V K+  HP Y     F   ND+ L+K     + N     +       N     G +C   GWG       +T   LQ+ASL +++N+DC K F+  +  D  ICAG   S AS+C GD GGPL C         VGIVS+G   C    +P V+ R++ +
Sbjct:   28 ISGYSRIVNGEEAVPHSWPWQVSLQDYTGFHFCGGSLINEWWVVTAAHC--NVRT---------SHRVILGEHDRSSNAESIQTMSVGKVFKHPQYNG---FTINNDILLIKLATPAQMNPRVSPVCVAATSDN--FPGGMKCVTSGWGLTKHNAPDTPAKLQQASLPLLTNDDC-KRFWGSQISDVMICAGA--SGASSCMGDSGGPLVCQK-SGAWTLVGIVSWGSGTC-TTSMPGVYARVTKL 252          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008289.1 (pep scaffold:Pmarinus_7.0:GL480450:20964:30734:1 gene:ENSPMAG00000007473.1 transcript:ENSPMAT00000008289.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 94.7449 bits (234), Expect = 4.139e-23
Identity = 70/239 (29.29%), Postives = 113/239 (47.28%), Query Frame = 1
Query:  139 INGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIE-NTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSN-ICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            + GS+   H  P   S++    HICG  L+  N V+T+AHC          Y      ++ LG++ +    G EK F V++++ H  + +  +    NDV LVK    +  N +   +      +     PG  C V GWG+L       + +L+  ++ ++SN  C+ T Y    I  N +CAG       AC+GD GGPL C D K +    G+VS+G + C +P    ++T +S +
Sbjct:   27 VGGSEVAPHAIPSQASLRYNGVHICGAALIRTNWVITSAHC----------YKAAQNLKVVLGDHSMSKADGTEKTFSVSRVVRHEKFNNVTN---DNDVMLVKLSANVALNANIQLVALQTSAL----PPGTLCNVSGWGTLQESSNKGSDVLRAVTVPIVSNALCNSTLYYRGLITVNMLCAGFDQGGKDACQGDSGGPLIC-DNKLK----GLVSWG-LGCARPRYFGIYTDVSRL 242          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006511.1 (pep scaffold:Pmarinus_7.0:GL476457:197255:208031:1 gene:ENSPMAG00000005872.1 transcript:ENSPMAT00000006511.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 86.2705 bits (212), Expect = 4.888e-20
Identity = 76/259 (29.34%), Postives = 122/259 (47.10%), Query Frame = 1
Query:   88 HLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDN----HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGD-RCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSN-ICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            HL  +     +S   + +NG +   H +P   S++        H+CGG+L+    V+T AHCI   R           +R+ LG+++L      + +  +   ++HPS+ +  +  +  D+AL+K       ++   IK  VL   G     +  C   GWG L   G+  + +LQEA L  ++   CS+  +    +++N ICAG    D + C GD GGPL C +     V  GI S+GH  C   + P VFTR+S 
Sbjct:   14 HLTSLTYLWHVSAQNRIVNGVEAAPHTWPWQASLQAQTGTGWYHVCGGSLVQQQWVMTAAHCIQADRV----------YRMVLGKHNLRINEPAQFESTIALSVVHPSWNNHPTRGW-FDIALMKLSSAAPLSEQ--IKLAVLPRVGQELWDNVTCMATGWGLLASDGKFPS-VLQEAKLPFVNYSTCSQQEWWWYGVNTNLICAG---DDHAVCNGDSGGPLSCQEPNGSWVVYGITSFGHKNCSVFHKPSVFTRVSA 255          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005493.1 (pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-1 gene:ENSPMAG00000004970.1 transcript:ENSPMAT00000005493.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 84.3445 bits (207), Expect = 2.307e-19
Identity = 73/244 (29.92%), Postives = 116/244 (47.54%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS---KPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNV-KYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            + +NG + +   +P  VS++     H CGG+L++   V+T AHC               + R+ LGE+D        +   + K I HP +   MS    NDVAL+K     + TD      + + ++  S    PG  C   GWG       +T   LQ+ +L +++   C +   N+ K  D  ICA  G + A++C+ D GGPL C      ++ VGIVS+G+  C    IP V+TR++ +
Sbjct:   26 RIVNGEEAMPGSWPWQVSLQSKTGFHFCGGSLIDPYWVVTAAHC----------GISPSEHRVILGEHDRSSSSEKVQTMTIAKAINHPQW-DNMSI--NNDVALIKLSQPAQMTD----GVSAVCLSSTSDNFPPGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLLANMNKITDVMICA--GGAGATSCRRDSGGPLVCEKDGAWNL-VGIVSWGNSSCST-RIPAVYTRVTAI 248          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002946.1 (pep scaffold:Pmarinus_7.0:GL477988:107792:112981:-1 gene:ENSPMAG00000002665.1 transcript:ENSPMAT00000002946.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.8037 bits (203), Expect = 1.126e-18
Identity = 73/244 (29.92%), Postives = 119/244 (48.77%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN---HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP-GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNV-KYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTV 849
            + + G + +   +P  VS  L +N   H CGG+L++   ++T AHC            +  + R+ LGE+D        +   + K+I HP +  ++SF   NDVAL+K     + TD   +    L     + P G  C   GWG       +T   LQ+ +L +++   C + F ++ K  D  ICAG   + AS+C+ D GGPL C      ++ VGIVS+G   C    +P+V+TR++ +
Sbjct:   35 RIVKGEEAMPGSWPWQVS--LQNNTGFHFCGGSLIDPYWIVTAAHC----------KIRPSEHRVILGEHDRSSSSEKVQTMTIAKVINHPQW-DDVSF--NNDVALIKLPQPAQMTDG--VSAVCLSSTSDNVPAGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLFASMNKITDVMICAG--GAGASSCRCDSGGPLVCKKNGAWNL-VGIVSWGSSVCST-SMPVVYTRVTAI 257          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: zmp:0000001088 (zmp:0000001088 [Source:ZFIN;Acc:ZDB-GENE-140106-48])

HSP 1 Score: 79.337 bits (194), Expect = 4.669e-18
Identity = 56/182 (30.77%), Postives = 85/182 (46.70%), Query Frame = 1
Query:  307 KFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS-KPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDC-SKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
               + LGE+ L    G E+  +V+K+I HPSY +       NDV L+K       +   Y+    L   G S   G  C V GWG+    G +    L    + V+S   C + + Y  +   + +CAG       +C+GD GGP+ C  G   H   G+VS+G+  C QP  P V+ R+++
Sbjct:    3 SMTVVLGEHSLSLNQGTEQVLKVSKVISHPSYNANT---MNNDVLLIKLQ--TAASLNSYVGTVGLPSQGASLSDGTSCRVSGWGNTRASGSSYPDKLMAVDVPVISTTTCNAGSAYGGQITANMVCAGFMAGGKDSCQGDSGGPMVC--GGLLH---GVVSWGY-GCAQPNKPGVYARVAS 173          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO44207 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW60])

HSP 1 Score: 99.3673 bits (246), Expect = 8.730e-25
Identity = 70/240 (29.17%), Postives = 122/240 (50.83%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASA-CKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            + ING     H +P  +S++     H+CGG L++   V+T +HCI       +D      + + +G + +     +EK  +V++++ HP Y S+      ND+AL++    +E      +    L  N    PG +C + GWG++    + +  +LQ+A L + S+E+C + + +V    +++CAG   SDA+  C GD GGPL C D  +  +  G VSYG   C   +   VF R+++
Sbjct:    2 RIINGQNAQPHSWPWQISLRQGRRFHLCGGALISDRWVVTASHCI-------HDDLNPGSYMVVVGAHRMSGSTSVEKTIRVSQVLEHPRYDSQRDV---NDIALIELATPVEFDSAGKVGTVCL-TNQRPAPGKQCYITGWGAVNGHTQQSPDILQQAMLPIASHENCRRKYGDVSST-AHLCAGEARSDAAGGCNGDSGGPLVCEDNGSWFLH-GAVSYGMKYCPTTHY-TVFARVAS 227          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO30908 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZF8])

HSP 1 Score: 98.5969 bits (244), Expect = 2.017e-24
Identity = 79/243 (32.51%), Postives = 127/243 (52.26%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVL---DINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDC-SKTFYNVKYID-SNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            + +NG     H +P  +S+++   HICGG+L+    VLT AHC+        D+     + + +G + +     I+++F++TK+  H  ++  MS +  NDVAL+K D  I+ +D    K N +   + N  ++ G +C + GWG L+  G+  + +LQ+A L V S+  C  K  Y V   + S ICAG        C+GD GGP  C +G  + V  G VS+G   CG  +   VF R+S+
Sbjct:   10 RVVNGDNAAQHSWPWQISLRVNGRHICGGSLITPEWVLTAAHCV-------EDFPHPSGYTVVVGAHRIKERSAIQQEFRLTKLFKHKDFS--MS-HLKNDVALLKLDNPIQPSD----KVNTVCLPERNSRAQVGAQCFITGWGRLVGGGQ-PSEILQQAMLPVASHSACDRKNSYLVPVEEKSMICAGG--MGMGGCQGDSGGPFVCNEG-GRWVLRGAVSWGQPMCGTDHY-TVFARVSS 233          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO33459 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SS64])

HSP 1 Score: 97.4413 bits (241), Expect = 6.150e-24
Identity = 72/247 (29.15%), Postives = 121/247 (48.99%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLM-----DNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISK----PGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            + ING     H +P  +S+++M     D H CGG+L++   VLT AHC+  +R          ++ +++G ++L     +E+   ++KI  H  Y+S +     +DVAL+K    +  + +      V   +G+S      G +C + GWG ++  G   A  LQ+A L+V S+ DC      +  +D       G+     C+GD GGP  C +G  + V  G VS+GHV C   +   VF R+++
Sbjct:    2 RVINGVDATAHAWPWQISLRMMSKKGDDYHFCGGSLIDSEWVLTAAHCVAGIR-------NPRRYSVYVGAHELDGTTQVEEKISISKIYSHEKYSSSL---LTSDVALIKLSKAVSLSKH---VNTVCLPSGLSSDEAPAGSKCFITGWGRMVAGGSG-ANTLQQADLLVASHSDCQARMGYMLSVDKATMICAGSQGKGGCQGDSGGPFVCEEG-GKWVLRGAVSWGHVNCLTDHY-TVFARVNS 232          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO34771 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNF5])

HSP 1 Score: 97.4413 bits (241), Expect = 6.822e-24
Identity = 73/240 (30.42%), Postives = 117/240 (48.75%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASA-CKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            + ING     H +P  +S++     H CGGTL++   V+T +HC+               + + +G ++ +    +++   V+ +I HP Y         ND+AL++    ++      +    L  N    PG RC + GWGS +  G N+ R+LQ+A L + S+ DC   +Y V    +++CAG   S AS  C GD GGPL C D    ++  G VSYG + C   Y   VF R+++
Sbjct:   16 RVINGQNAQPHSWPWQISLRPYGRYHSCGGTLISDRWVVTASHCV--------HKNPRPSYTVVVGAHERNGKTAVQESIPVSHVIEHPEYDDRK---IKNDIALLELSRPVKFDREGKVGTACL-TNQQPTPGKRCYITGWGSTIGTG-NSPRILQQAMLPIASHNDCKNKYYGVSST-AHLCAGEARSGASGGCNGDSGGPLVCEDNGRWYLH-GAVSYGKLHCPTTYY-TVFARVAS 239          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO44208 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW61])

HSP 1 Score: 92.0485 bits (227), Expect = 3.999e-22
Identity = 69/240 (28.75%), Postives = 120/240 (50.00%), Query Frame = 1
Query:  133 KTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDAS-ACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            + ING     H +P  +S++     H CGG+L++   V+T AHCI    + N +   Y    + +G +       +++  ++++II HP Y         ND+AL++    ++      +    L  +    PG RC + GWG++    + +  +LQ+A L + S+E+C + + +V    +++CAG   SDA+  C GD GGPL C D  +  +  G VSYG   C   +   VF R+++
Sbjct:    2 RIINGQNAQPHSWPWQISLRGRSGFHTCGGSLISDRWVVTAAHCI----HRNKNPGGYT---VVVGAHKKRGSTSVQQSLRLSQIIEHPKYNDRRIV---NDIALIELATPVQFDSAGKVGTVCL-TDQRPAPGKRCYITGWGAINGDTQQSPDILQQAMLPIASHENCRRKYGDVSST-AHLCAGEARSDAAGGCNGDSGGPLVCEDNGSWFLH-GAVSYGLRNCPTTHY-TVFARVAS 227          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: GZMM (duodenase-1 [Source:NCBI gene;Acc:101164468])

HSP 1 Score: 104.375 bits (259), Expect = 3.879e-26
Identity = 83/255 (32.55%), Postives = 122/255 (47.84%), Query Frame = 1
Query:   91 LFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDI-NGISKPGDRCEVVGWGSLLPPGENT--ARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            +FV+L F   +     + G     H  P M S+++   HICGG L+  + VLT AHC  DL            F + LG   L       + F+V + I HP+Y +E+S    ND+ L+K DG    T     +  V+ + +G   P  +C   GWG +   G+N   A  LQE  + +++   C + + NV+ + S +C     S    C GD GGP+ C DG+      GIVS+    CG P  P V+TRIS+
Sbjct:    7 MFVLLFFMGGAAGSHIVGGRDAAPHSRPYMASLQVRGQHICGGALVREDFVLTAAHC--DL---------PIPFTVVLGADLLQENEPSRQTFRVLRSIPHPNY-NELS----NDIMLLKLDGRARLTP----EVQVIPLKSGTVGPASQCITAGWGDI---GDNRTIANRLQEVDVTILAQRTCRQRWGNVRVLRSMVCGVGARSFQGFCSGDSGGPMIC-DGE----AAGIVSFSGRRCGDPSTPDVYTRISS 233          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: zgc:112285 (elastase-1 [Source:NCBI gene;Acc:101161025])

HSP 1 Score: 97.4413 bits (241), Expect = 5.892e-23
Identity = 80/260 (30.77%), Postives = 128/260 (49.23%), Query Frame = 1
Query:  103 LSFCAISTALKTINGSQEIMHQFPQMVSIKLMDN------HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGIS-KPGDRCEVVGWGSLLPPGENT--ARLLQEASLIVMSNEDC-SKTFYNVKYIDSNICAGTGNSD--ASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIST 846
            +++   + A + ++G++   H +P  VS+++         H+CGGTL++ N VLT AHC     +          +RI LG++ L      E+ F V +I  H S+          D+ALVK    I  +++  I+Y  L       +PG  C V GWG      +N   A  L +A L ++  + C  K F+  +  DS ICAG  +++   +AC+GD GGPL C   +++    G+VS+G + C     P VFTR +T
Sbjct:   67 MAYFKPNMAERIVSGNEARPHSWPWQVSLQVRPRGSKHYVHVCGGTLIHKNWVLTAAHC-----FQKGKAEDAGSWRIVLGKHQLKRSETAERIFPVKRIYRHESFRYPTHSELDYDIALVKAGTDIPPSNF--IRYACLPRKQTGLRPGHYCWVTGWGDTRGGKDNVSLAEALNQARLPIIDFKTCRQKKFWGDRVRDSMICAGFRDTEDPPAACQGDSGGPLLCQLDRDRWEVHGVVSFGPIGCTVENKPSVFTRTAT 319          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: zgc:112160 (chymotrypsin B [Source:NCBI gene;Acc:105356289])

HSP 1 Score: 92.0485 bits (227), Expect = 1.899e-21
Identity = 81/262 (30.92%), Postives = 124/262 (47.33%), Query Frame = 1
Query:   91 LFVVLSFCA-------ISTALKTINGSQEIMHQFPQMVSIKLMDN-HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQV---TKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRIS 843
            LFV+L  C        ++   + +NG + + H +P  VS++  +  H CGG+L+N N V+T AHC         +   Y+  R+  GE++     G  +D Q+    ++  HP +         ND+AL+K      N         + + +    PG  C   GWG       +T   LQ+ASL ++SN+DC K ++     D  ICA  G + A++C GD GGPL CT   N    VGIVS+G   C     P V+ R++
Sbjct:   15 LFVLLPGCGTPSIPPRVTHQARIVNGEEAVPHSWPWQVSLQQTNGFHFCGGSLINENWVVTAAHC---------NVRTYH--RVIAGEHN--KGAGSNEDIQILTPAQVFTHPQWNPST---INNDIALIKL-ASPANLGAHVSPVCLAESSDYFAPGSTCVTSGWGLTHHLAPSTPNNLQQASLPLLSNDDC-KLYWGSNISDVMICA--GGAGATSCMGDSGGPLVCTK-DNVWTLVGIVSWGSSSCST-NTPAVYARVT 254          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ela2l (chymotrypsin-like elastase family member 2A [Source:NCBI gene;Acc:101168781])

HSP 1 Score: 92.0485 bits (227), Expect = 2.529e-21
Identity = 85/268 (31.72%), Postives = 134/268 (50.00%), Query Frame = 1
Query:   79 VVFHLFVVLSF-CAIST---ALKTINGSQEIM-HQFPQMVSIKLMDN----HICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKP-GDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKT-FYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGH-VPCGQPYIPIVFTRIST 846
            +V  LFV  ++ C + T    L  + G Q++  + +P  +S++   N    H CGGTL++   VLT AHCI   R          ++R+ LG+++L              II+H +++S    +  ND+AL+K +  +E +D   I    L   G   P  + C V GWG +   G   A +LQ+A L V+ +  CSK+ ++  +  ++ +CAG G+   S C GD GGPL C          GIVS+G  + C  P  P VFT++S+
Sbjct:    4 LVLALFVAGAYGCGLPTFPPILGRVVGGQDVRPNSWPWQISLQYNSNGEWRHTCGGTLISDQWVLTAAHCISSGR----------EYRVALGKHNLPETEDGSVFKSTANIIVHENWSS---LFIRNDIALIKLESPVEFSDS--IMAACLPAAGFLLPHNESCFVTGWGRIYTGGP-IADILQQALLPVVDHATCSKSDWWGFQVTETMVCAG-GDGVVSGCNGDSGGPLNCQAADGSWEVHGIVSFGSGLSCNYPKKPTVFTQVSS 254          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000023230.2 (tryptase [Source:NCBI gene;Acc:101157190])

HSP 1 Score: 89.3521 bits (220), Expect = 2.094e-20
Identity = 76/246 (30.89%), Postives = 117/246 (47.56%), Query Frame = 1
Query:  139 INGSQEIMHQFPQMVSIKLMDNHI----CXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSL-----LPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHV-PCGQPYIPIVFTRIST 846
            I G      ++P MV + +  + +    CGG+L+    VLT AHC WD            +  +++G  DL +  G E+  +VT +I HP Y      Y  ND+AL++ D  +  +       +++  +    P   C + GWG +     LP  E     LQ+  + ++   DC +T+  +   D  +CAG+      ACKGD GGPL C   +   VQVGI+S G    CG P  P V+TR+S+
Sbjct:   32 IGGEDAAEGKWPWMVHLNITSDGLKRWRCGGSLVAKQWVLTAAHC-WDPSL----SVNLRRSGVWVGARDLSH--GAERYRRVTHVISHPGYEGGGGVYK-NDLALLRLDKALAFSQR-VAPLSLVQTDDAFGPSSECWITGWGLVGNGVPLPDPET----LQQLQISIVPQRDCRQTYPWL--TDDMLCAGSPAGGKDACKGDYGGPLVCRTPRG-FVQVGIMSSGSPDGCGLPGRPGVYTRVSS 261          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000042386.1 (SMESG000042386.1)

HSP 1 Score: 517.694 bits (1332), Expect = 0.000e+0
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 1
Query:   67 MSQKVVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 852
            MSQKVVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICGGTLLNGNTVLTTAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA
Sbjct:    1 MSQKVVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICGGTLLNGNTVLTTAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 262          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000042388.1 (SMESG000042388.1)

HSP 1 Score: 325.094 bits (832), Expect = 2.679e-112
Identity = 152/231 (65.80%), Postives = 193/231 (83.55%), Query Frame = 1
Query:  160 MHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 852
            MH++PQMVSIK+  +H CGGTLLNGNTVLT AHC+W +R WN + FK Y  +I+LGEYDL+ MYG+EK FQVTK+I+HPSYTS+ ++ YGNDVAL+KFDGY+ NT Y+YIKY  LD+N I+KPGDRCEVVGWGS+LPPG++T+R LQ+ ++ ++SN +CS+ F NV+Y+ SN+CAG+G+   SACKGD GGPLFCTDG+  H+QVGIVSYG VPCGQ  +P VFTR+S VA
Sbjct:    1 MHEYPQMVSIKVQGSHFCGGTLLNGNTVLTAAHCVWSMRNWNLNTFKSYSMKIYLGEYDLYQMYGVEKIFQVTKVIIHPSYTSDFAYVYGNDVALIKFDGYVSNTQYNYIKYGELDLNSITKPGDRCEVVGWGSMLPPGQDTSRFLQKTAINILSNSECSRIFTNVRYVASNVCAGSGSLSTSACKGDSGGPLFCTDGRRHHIQVGIVSYGRVPCGQANVPTVFTRVSEVA 231          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000042349.1 (SMESG000042349.1)

HSP 1 Score: 321.242 bits (822), Expect = 1.117e-110
Identity = 151/231 (65.37%), Postives = 192/231 (83.12%), Query Frame = 1
Query:  160 MHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 852
            MH++PQMVSIK+  +H CGGTLLNGNTVLT AHC+W +R WN + FK Y  +I+LGEYDL+ MYG+EK FQVTK+I+HPSYTS+ ++ YGNDVAL+KFDGY+ NT Y+YIKY  LD+N I+KPGDRCEVVGWGS+LPPG++T+R LQ+A++ ++S  +CS+ F NV Y+ SN+CAG+G+   SAC+GD GGPLFCTDG+  H+QVGIVSYG VPCGQ  +P VFTR+S VA
Sbjct:    1 MHEYPQMVSIKVQGSHSCGGTLLNGNTVLTAAHCVWSMRNWNLNTFKSYSMKIYLGEYDLYQMYGVEKIFQVTKVIIHPSYTSDFAYVYGNDVALIKFDGYVSNTQYNYIKYGELDLNSITKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINILSTSECSRIFTNVWYVASNVCAGSGSLSTSACRGDSGGPLFCTDGRRHHIQVGIVSYGQVPCGQANVPTVFTRVSEVA 231          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000042389.1 (SMESG000042389.1)

HSP 1 Score: 270.396 bits (690), Expect = 1.189e-90
Identity = 137/231 (59.31%), Postives = 178/231 (77.06%), Query Frame = 1
Query:  160 MHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVA 852
            MHQ+PQ+VSI+L  +HICGGTLLNGNTVLT AHC+WD+RY+  D+F+Y+K ++ LGEYDLH++ G EK  QV KI+MHP+Y    ++  GNDVAL+KFDG+I N + +Y+KY  LD +    PG RCEVVGWGS+ P GE+T++ LQ+A L ++S E+CSKT+ NV+ + SNICAG+G+   SACKGD GGPLFC D  N+ +QVGIVSY  +PCGQ   P V+TRIS VA
Sbjct:    1 MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVA 231          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000042396.1 (SMESG000042396.1)

HSP 1 Score: 174.096 bits (440), Expect = 1.224e-53
Identity = 96/180 (53.33%), Postives = 123/180 (68.33%), Query Frame = 1
Query:   79 VVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICXXXXXXXXXXXXXAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIV 618
            +VF +F+      +S   K ING+Q I+H++PQMVSI+L+  HICGGTLLN NTVLT AHCIW +  W  +  K YK  I LGEYDL  + G EK FQVTK ++HP YT EMS+  GNDV L+KFDG ++N ++ YIKY         + G+RCEVVGWGS+ P G  ++R LQ+ASL V
Sbjct:    8 IVFLIFI----STVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNV 183          
The following BLAST results are available for this feature:
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KLK135.080e-2632.46kallikrein related peptidase 13 [Source:HGNC Symbo... [more]
CTRL1.183e-2131.97chymotrypsin like [Source:HGNC Symbol;Acc:HGNC:252... [more]
GZMM2.735e-2128.63granzyme M [Source:HGNC Symbol;Acc:HGNC:4712][more]
CELA2A1.924e-2032.38chymotrypsin like elastase 2A [Source:HGNC Symbol;... [more]
KLK88.544e-2029.92kallikrein related peptidase 8 [Source:HGNC Symbol... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
svh-12.186e-1124.63HGF/MSP/plasminogen-like protein [Source:UniProtK... [more]
try-13.058e-1126.23TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-31.666e-828.81TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-31.722e-828.81TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tpr7.412e-2429.84gene:FBgn0034661 transcript:FBtr0071743[more]
CG187353.456e-1927.56gene:FBgn0042098 transcript:FBtr0071744[more]
CG74323.914e-1833.33gene:FBgn0038727 transcript:FBtr0301213[more]
CG93724.526e-1731.55gene:FBgn0036891 transcript:FBtr0074945[more]
lambdaTry9.300e-1727.76gene:FBgn0043470 transcript:FBtr0088121[more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:1122853.245e-2531.29zgc:112285 [Source:ZFIN;Acc:ZDB-GENE-050913-132][more]
si:ch73-182e20.45.843e-2233.73si:ch73-182e20.4 [Source:ZFIN;Acc:ZDB-GENE-131127-... [more]
si:ch73-182e20.41.862e-2035.02si:ch73-182e20.4 [Source:ZFIN;Acc:ZDB-GENE-131127-... [more]
ctrl3.657e-2031.80chymotrypsin-like [Source:ZFIN;Acc:ZDB-GENE-040912... [more]
zgc:558882.753e-1930.83zgc:55888 [Source:ZFIN;Acc:ZDB-GENE-040426-834][more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
noxa11.691e-2331.39NADPH oxidase activator 1 [Source:Xenbase;Acc:XB-G... [more]
noxa11.149e-2231.54NADPH oxidase activator 1 [Source:Xenbase;Acc:XB-G... [more]
ENSXETT00000015681.12.009e-2232.03pep primary_assembly:Xenopus_tropicalis_v9.1:9:272... [more]
ctrl1.214e-2130.00chymotrypsin-like [Source:NCBI gene;Acc:548358][more]
TMPRSS91.537e-2132.64transmembrane serine protease 9 [Source:NCBI gene;... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gzmm6.831e-2531.67granzyme M (lymphocyte met-ase 1) [Source:MGI Symb... [more]
Klk82.425e-2431.01kallikrein related-peptidase 8 [Source:MGI Symbol;... [more]
Klk134.234e-2230.00kallikrein related-peptidase 13 [Source:MGI Symbol... [more]
Klk1b95.229e-2232.06kallikrein 1-related peptidase b9 [Source:MGI Symb... [more]
Tmprss91.602e-2132.51transmembrane protease, serine 9 [Source:MGI Symbo... [more]
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BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9UKR3|KLK13_HUMAN2.440e-2532.46Kallikrein-13 OS=Homo sapiens OX=9606 GN=KLK13 PE=... [more]
sp|Q61955|KLK8_MOUSE1.696e-2331.01Kallikrein-8 OS=Mus musculus OX=10090 GN=Klk8 PE=1... [more]
sp|Q03238|GRAM_RAT1.043e-2231.51Granzyme M (Fragment) OS=Rattus norvegicus OX=1011... [more]
sp|P00774|CEL2A_RAT1.361e-2232.79Chymotrypsin-like elastase family member 2A OS=Rat... [more]
sp|O88780|KLK8_RAT2.894e-2228.63Kallikrein-8 OS=Rattus norvegicus OX=10116 GN=Klk8... [more]
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BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DRI73.139e-8761.86Trypsin-like serine protease OS=Dugesia japonica O... [more]
A0A1I8JAW51.796e-3437.40Peptidase S1 domain-containing protein OS=Macrosto... [more]
A0A1I8GT162.341e-3437.40Peptidase S1 domain-containing protein OS=Macrosto... [more]
U5EP401.418e-2532.05Putative trypsin-like serine protease OS=Corethrel... [more]
A0A0L8HIV64.297e-2531.87Uncharacterized protein OS=Octopus bimaculoides OX... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000043830.11.355e-2537.61elastase-1-like [Source:NCBI gene;Acc:103036513][more]
ENSAMXT00000039451.15.915e-2231.69trypsin-like [Source:NCBI gene;Acc:111192060][more]
ENSAMXT00000048530.14.337e-2127.27pep primary_assembly:Astyanax_mexicanus-2.0:13:202... [more]
zgc:1121601.860e-2032.50chymotrypsin B-like [Source:NCBI gene;Acc:10302813... [more]
ctrb13.338e-2032.11chymotrypsin A-like [Source:NCBI gene;Acc:10302748... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000008289.14.139e-2329.29pep scaffold:Pmarinus_7.0:GL480450:20964:30734:1 g... [more]
ENSPMAT00000006511.14.888e-2029.34pep scaffold:Pmarinus_7.0:GL476457:197255:208031:1... [more]
ENSPMAT00000005493.12.307e-1929.92pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-... [more]
ENSPMAT00000002946.11.126e-1829.92pep scaffold:Pmarinus_7.0:GL477988:107792:112981:-... [more]
zmp:00000010884.669e-1830.77zmp:0000001088 [Source:ZFIN;Acc:ZDB-GENE-140106-48... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO442078.730e-2529.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO309082.017e-2432.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO334596.150e-2429.15Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347716.822e-2430.42Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO442083.999e-2228.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GZMM3.879e-2632.55duodenase-1 [Source:NCBI gene;Acc:101164468][more]
zgc:1122855.892e-2330.77elastase-1 [Source:NCBI gene;Acc:101161025][more]
zgc:1121601.899e-2130.92chymotrypsin B [Source:NCBI gene;Acc:105356289][more]
ela2l2.529e-2131.72chymotrypsin-like elastase family member 2A [Sourc... [more]
ENSORLT00000023230.22.094e-2030.89tryptase [Source:NCBI gene;Acc:101157190][more]
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BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042386.10.000e+0100.00SMESG000042386.1[more]
SMESG000042388.12.679e-11265.80SMESG000042388.1[more]
SMESG000042349.11.117e-11065.37SMESG000042349.1[more]
SMESG000042389.11.189e-9059.31SMESG000042389.1[more]
SMESG000042396.11.224e-5353.33SMESG000042396.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007489 ID=SMED30007489|Name=Peptidase S1 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=927bp
CGGAATATCGTCAAAAAGTATTTAATCACAAACAACTAATCATAATTATT
TTATCTCTACTCTAAAATGAGTCAAAAAGTAGTTTTCCATTTATTTGTTG
TCTTGTCGTTTTGTGCAATTTCAACAGCACTGAAAACAATTAACGGCAGT
CAGGAAATAATGCACCAATTTCCTCAGATGGTCTCCATAAAACTGATGGA
TAACCATATCTGTGGAGGGACTTTACTGAATGGCAACACTGTTCTGACCA
CTGCTCACTGTATTTGGGATCTGCGTTATTGGAACAATGACTACTTTAAA
TACTATAAATTTCGAATCTTTTTAGGAGAATACGATTTACACTATATGTA
TGGCATAGAAAAAGATTTCCAAGTCACAAAGATAATTATGCATCCTTCAT
ATACTAGCGAAATGTCATTTTATTATGGGAATGACGTCGCACTGGTTAAA
TTTGACGGATATATAGAGAATACGGATTATGATTACATTAAATATAATGT
TTTGGATATTAATGGAATATCTAAACCGGGCGATCGTTGTGAAGTTGTTG
GTTGGGGAAGTTTGTTACCCCCCGGAGAAAATACTGCTCGACTTTTACAG
GAAGCTAGTCTTATAGTTATGTCCAATGAAGATTGTTCCAAAACGTTTTA
TAACGTCAAGTACATTGATAGTAATATTTGTGCTGGTACAGGAAATTCTG
ACGCTTCTGCTTGTAAGGGTGATTTAGGAGGACCTCTTTTTTGCACCGAC
GGAAAAAATCAACATGTTCAAGTTGGAATAGTAAGTTATGGACATGTACC
TTGTGGACAGCCATACATTCCAATAGTTTTCACTCGAATTTCAACTGTTG
CAAAATGGATTATTGACAGTTCTAAATAAATATTAATATCAAAATTTAAA
TAACTTCGTGGAATTATAATAACAAAA
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protein sequence of SMED30007489-orf-1

>SMED30007489-orf-1 ID=SMED30007489-orf-1|Name=SMED30007489-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=271bp
MSQKVVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICG
GTLLNGNTVLTTAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKD
FQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDING
ISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYI
DSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPY
IPIVFTRISTVAKWIIDSSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0008236serine-type peptidase activity
GO:0016787hydrolase activity
GO:0004252serine-type endopeptidase activity
GO:0008233peptidase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR001314Peptidase_S1A
IPR001254Trypsin_dom
IPR009003Peptidase_S1_PA
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001314Peptidase S1A, chymotrypsin familyPRINTSPR00722CHYMOTRYPSINcoord: 213..225
score: 54.62
coord: 119..133
score: 25.82
coord: 50..65
score: 53.21
IPR001254Serine proteases, trypsin domainSMARTSM00020trypsin_2coord: 23..265
e-value: 1.1E-55
score: 201.0
IPR001254Serine proteases, trypsin domainPFAMPF00089Trypsincoord: 25..265
e-value: 2.1E-44
score: 152.0
IPR001254Serine proteases, trypsin domainPROSITEPS50240TRYPSIN_DOMcoord: 24..270
score: 27.797
IPR001254Serine proteases, trypsin domainCDDcd00190Tryp_SPccoord: 25..265
e-value: 5.14471E-59
score: 185.558
NoneNo IPR availableGENE3DG3DSA:2.40.10.10coord: 36..265
e-value: 3.1E-55
score: 189.2
NoneNo IPR availableGENE3DG3DSA:2.40.10.10coord: 25..259
e-value: 3.1E-55
score: 189.2
NoneNo IPR availablePANTHERPTHR24253FAMILY NOT NAMEDcoord: 24..267
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.818
NoneNo IPR availableTMHMMTMhelixcoord: 5..22
IPR009003Peptidase S1, PA clanSUPERFAMILYSSF50494Trypsin-like serine proteasescoord: 9..267