Coiled coil domain containing protein 58

Overview
NameCoiled coil domain containing protein 58
Smed IDSMED30007465
Length (bp)505
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Coiled coil domain containing protein 58 (SMED30007465) t-SNE clustered cells

Violin plots show distribution of expression levels for Coiled coil domain containing protein 58 (SMED30007465) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Coiled coil domain containing protein 58 (SMED30007465) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Coiled coil domain containing protein 58 (SMED30007465) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007465

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30007465h1SMcG0019611 SmedASXL_018873SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
muscle cellSMED30007465h1SMcG0019611 dd_Smed_v4_1983_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30007465h1SMcG0019611 dd_Smed_v4_1983_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30007465h1SMcG0019611 dd_Smed_v4_1983_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30007465h1SMcG0019611 dd_Smed_v4_1983_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Human
Match: CCDC58 (coiled-coil domain containing 58 [Source:HGNC Symbol;Acc:HGNC:31136])

HSP 1 Score: 105.916 bits (263), Expect = 3.277e-29
Identity = 50/131 (38.17%), Postives = 86/131 (65.65%), Query Frame = 2 
Query:   68 CDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            C++F +F++LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H  R++ IKNCI   ++ ++++    +KN  D  +  ++R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:   10 CEEFAEFQELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHASRDRVIKNCIAQTSAVVKNLREEREKNLDDLTLLKQLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRIHFKP 140
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Human
Match: CCDC58 (coiled-coil domain containing 58 [Source:HGNC Symbol;Acc:HGNC:31136])

HSP 1 Score: 100.908 bits (250), Expect = 2.693e-27
Identity = 51/135 (37.78%), Postives = 84/135 (62.22%), Query Frame = 2 
Query:   56 ELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            EL+   D    K+LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H  R++ IKNCI   ++ ++++    +KN  D  +  ++R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:    3 ELVSVMDLSLAKELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHASRDRVIKNCIAQTSAVVKNLREEREKNLDDLTLLKQLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRIHFKP 137
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Human
Match: CCDC58 (coiled-coil domain containing 58 [Source:HGNC Symbol;Acc:HGNC:31136])

HSP 1 Score: 97.8265 bits (242), Expect = 3.174e-26
Identity = 47/125 (37.60%), Postives = 81/125 (64.80%), Query Frame = 2 
Query:   86 FKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            +++LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H  R++ IKNCI   ++ ++++    +KN  D  +  ++R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:    2 WQELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHASRDRVIKNCIAQTSAVVKNLREEREKNLDDLTLLKQLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRIHFKP 126
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Fly
Match: Ccdc58 (gene:FBgn0036909 transcript:FBtr0074880)

HSP 1 Score: 87.4261 bits (215), Expect = 2.233e-22
Identity = 49/129 (37.98%), Postives = 78/129 (60.47%), Query Frame = 2 
Query:   68 CDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSS---DGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYY 445
            C DF  F++ L   R  DD+I   LN ALPT+SF  ++N++S C+  Y ++  SHL R+++I++CI    S L+ + +K  +   D   D++ R E   ++  +AEL  E+II+D S K F +RCR Y+
Sbjct:    5 CLDFLGFQEALKKMRDVDDKIIYALN-ALPTESFKGQVNSESTCRDLYAKLQESHLARQESIRSCITISASNLKKLREKRETQPDDVDTDNQFRAEQRKLRVLQAELNVEDIIKDRSYKTFNERCRSYF 132
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Fly
Match: Ccdc58 (gene:FBgn0036909 transcript:FBtr0074879)

HSP 1 Score: 77.7962 bits (190), Expect = 9.167e-19
Identity = 44/115 (38.26%), Postives = 71/115 (61.74%), Query Frame = 2 
Query:  110 RKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSS---DGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYY 445
            R  DD+I   LN ALPT+SF  ++N++S C+  Y ++  SHL R+++I++CI    S L+ + +K  +   D   D++ R E   ++  +AEL  E+II+D S K F +RCR Y+
Sbjct:    2 RDVDDKIIYALN-ALPTESFKGQVNSESTCRDLYAKLQESHLARQESIRSCITISASNLKKLREKRETQPDDVDTDNQFRAEQRKLRVLQAELNVEDIIKDRSYKTFNERCRSYF 115
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Fly
Match: Ccdc58 (gene:FBgn0036909 transcript:FBtr0344256)

HSP 1 Score: 77.7962 bits (190), Expect = 9.167e-19
Identity = 44/115 (38.26%), Postives = 71/115 (61.74%), Query Frame = 2 
Query:  110 RKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSS---DGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYY 445
            R  DD+I   LN ALPT+SF  ++N++S C+  Y ++  SHL R+++I++CI    S L+ + +K  +   D   D++ R E   ++  +AEL  E+II+D S K F +RCR Y+
Sbjct:    2 RDVDDKIIYALN-ALPTESFKGQVNSESTCRDLYAKLQESHLARQESIRSCITISASNLKKLREKRETQPDDVDTDNQFRAEQRKLRVLQAELNVEDIIKDRSYKTFNERCRSYF 115
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Zebrafish
Match: ccdc58 (coiled-coil domain containing 58 [Source:NCBI gene;Acc:393780])

HSP 1 Score: 109.383 bits (272), Expect = 1.014e-30
Identity = 59/145 (40.69%), Postives = 92/145 (63.45%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILK----KNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIK 463
            M     +L C+DF  F+++L   R  DDRI + LN+ +PT SFS K++A   C++ YE ++ +HL R+K IK+CI+ +TS + S L+    K+S +  V  ++R+E   +K  ++EL  EE++ D SLKVF +RCR +Y P  IK
Sbjct:    1 MAAPGGVLNCEDFSLFQEVLKVMRTIDDRIVHSLNTTVPTASFSGKVDATQTCKELYESLMEAHLSRDKAIKSCIK-ETSAVVSQLREERAKDSDNLSVIKQLRKEQTKLKLMQSELNVEEVVNDRSLKVFNERCRIHYTPPKIK 144
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Mouse
Match: Ccdc58 (coiled-coil domain containing 58 [Source:MGI Symbol;Acc:MGI:2146423])

HSP 1 Score: 107.842 bits (268), Expect = 4.779e-30
Identity = 51/131 (38.93%), Postives = 86/131 (65.65%), Query Frame = 2 
Query:   68 CDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            C++F +F++LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H+ R++ IKNCI   ++ ++S+    +KN  D  +   +R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:   10 CEEFAEFQELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHVSRDRVIKNCIAQTSAVVKSLREEREKNLDDLTLLKRLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRVHFKP 140
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Mouse
Match: Ccdc58 (coiled-coil domain containing 58 [Source:MGI Symbol;Acc:MGI:2146423])

HSP 1 Score: 98.2117 bits (243), Expect = 1.938e-26
Identity = 52/142 (36.62%), Postives = 83/142 (58.45%), Query Frame = 2 
Query:   35 LDMNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            +D  D NELL               R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H+ R++ IKNCI   ++ ++S+    +KN  D  +   +R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:    1 MDSTDANELLKV------------MRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHVSRDRVIKNCIAQTSAVVKSLREEREKNLDDLTLLKRLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRVHFKP 130
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Mouse
Match: Ccdc58 (coiled-coil domain containing 58 [Source:MGI Symbol;Acc:MGI:2146423])

HSP 1 Score: 62.7734 bits (151), Expect = 5.418e-13
Identity = 31/89 (34.83%), Postives = 57/89 (64.04%), Query Frame = 2 
Query:  197 CQKFYE-EMITSHLYREKTIKNCIRNKTSYLESILK---KNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            C++F E +++ +H+ R++ IKNCI   ++ ++S+ +   KN  D  +   +R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:   10 CEEFAEFQLMAAHVSRDRVIKNCIAQTSAVVKSLREEREKNLDDLTLLKRLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRVHFKP 98
[Close]
BLAST of Coiled coil domain containing protein 58 vs. UniProt/SwissProt
Match: sp|Q6PBK1|CCD58_DANRE (Coiled-coil domain-containing protein 58 OS=Danio rerio OX=7955 GN=ccdc58 PE=2 SV=2)

HSP 1 Score: 109.383 bits (272), Expect = 7.655e-30
Identity = 59/145 (40.69%), Postives = 92/145 (63.45%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILK----KNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIK 463
            M     +L C+DF  F+++L   R  DDRI + LN+ +PT SFS K++A   C++ YE ++ +HL R+K IK+CI+ +TS + S L+    K+S +  V  ++R+E   +K  ++EL  EE++ D SLKVF +RCR +Y P  IK
Sbjct:    1 MAAPGGVLNCEDFSLFQEVLKVMRTIDDRIVHSLNTTVPTASFSGKVDATQTCKELYESLMEAHLSRDKAIKSCIK-ETSAVVSQLREERAKDSDNLSVIKQLRKEQTKLKLMQSELNVEEVVNDRSLKVFNERCRIHYTPPKIK 144
[Close]
BLAST of Coiled coil domain containing protein 58 vs. UniProt/SwissProt
Match: sp|Q8R3Q6|CCD58_MOUSE (Coiled-coil domain-containing protein 58 OS=Mus musculus OX=10090 GN=Ccdc58 PE=1 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.343e-29
Identity = 51/131 (38.93%), Postives = 86/131 (65.65%), Query Frame = 2 
Query:   68 CDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            C++F +F++LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H+ R++ IKNCI   ++ ++S+    +KN  D  +   +R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:   10 CEEFAEFQELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHVSRDRVIKNCIAQTSAVVKSLREEREKNLDDLTLLKRLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRVHFKP 140
[Close]
BLAST of Coiled coil domain containing protein 58 vs. UniProt/SwissProt
Match: sp|B5X9S3|CCD58_SALSA (Coiled-coil domain-containing protein 58 OS=Salmo salar OX=8030 GN=ccdc58 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 8.216e-29
Identity = 52/144 (36.11%), Postives = 85/144 (59.03%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGPVDSEV---RREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIK 463
            M   +  L C+DF  F+ +L   R  DDRI + LN+ +PT SFS +++A   C++ YE ++ +HL R++ IK CI   +  +  + ++ + DG   + V   R+E   +K  ++EL  EE++ D SLKVF +RCR +Y P  +K
Sbjct:    1 MAAPDGTLNCEDFSMFQDVLKVMRNIDDRIVHSLNTTVPTVSFSGRVDASQTCRQLYESLMEAHLSRDRAIKTCIAQTSEVVGQLREQRAKDGDNMTTVKLLRKEQTKLKLMRSELNVEEVVNDRSLKVFSERCRTHYTPPTVK 144
[Close]
BLAST of Coiled coil domain containing protein 58 vs. UniProt/SwissProt
Match: sp|Q4VC31|CCD58_HUMAN (Coiled-coil domain-containing protein 58 OS=Homo sapiens OX=9606 GN=CCDC58 PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.574e-28
Identity = 50/131 (38.17%), Postives = 86/131 (65.65%), Query Frame = 2 
Query:   68 CDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            C++F +F++LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H  R++ IKNCI   ++ ++++    +KN  D  +  ++R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:   10 CEEFAEFQELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMAAHASRDRVIKNCIAQTSAVVKNLREEREKNLDDLTLLKQLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRIHFKP 140
[Close]
BLAST of Coiled coil domain containing protein 58 vs. UniProt/SwissProt
Match: sp|A4FUI1|CCD58_BOVIN (Coiled-coil domain-containing protein 58 OS=Bos taurus OX=9913 GN=CCDC58 PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.933e-28
Identity = 51/131 (38.93%), Postives = 85/131 (64.89%), Query Frame = 2 
Query:   68 CDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            C++F +F++LL   R  DDRI +ELN+ +PT SF+ KI+A   C++ YE ++ +H  R++ IKNCI   +S ++ +    +KN  D  +  ++R+E   +K  ++EL  EE++ D S KVF +RCR ++KP
Sbjct:   10 CEEFAEFQELLKVMRTIDDRIVHELNTTVPTASFAGKIDASQTCKQLYESLMEAHASRDRVIKNCIAQTSSVVKQLREEREKNLDDLTLLKQLRKEQTKLKWMQSELNVEEVVNDRSWKVFNERCRIHFKP 140
[Close]
BLAST of Coiled coil domain containing protein 58 vs. TrEMBL
Match: A0A0R3UM79 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8843 PE=4 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.932e-35
Identity = 63/139 (45.32%), Postives = 92/139 (66.19%), Query Frame = 2 
Query:   62 LPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESI---LKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKF 469
            +PC+DF +F KLL   RK+DDRINNELN+ALPT SFS+ ++A   C  FY +++  H  R   IK CI   T  ++ +   L+K+S    + + +R+E   ++QF +EL EEEIIQ+S+LKV Y+RCRD++     +KF
Sbjct:    9 IPCNDFLRFSKLLHTWRKSDDRINNELNAALPTASFSQSVDAAQQCSTFYNKLMDLHKRRFNAIKRCISVSTDRVKDLKQQLEKDSGSTSLSTAIRKEQLQLRQFNSELLEEEIIQNSALKVLYERCRDHFNSTAFEKF 147
[Close]
BLAST of Coiled coil domain containing protein 58 vs. TrEMBL
Match: A0A564YH00 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS6801 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 3.293e-35
Identity = 65/139 (46.76%), Postives = 92/139 (66.19%), Query Frame = 2 
Query:   62 LPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCI---RNKTSYLESILKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKF 469
             PCDDFF+F K L   RK+DDRINNELN+ALPT SFS  ++A   C  F+E+++  H  R   I+ CI    N+   L++  +K+SS   +   +R+E   ++QFK+EL EEEIIQ+S+LKV Y+RCRD++     +KF
Sbjct:    9 FPCDDFFRFSKALQTARKSDDRINNELNAALPTASFSNSVDATRQCSMFFEKLVDLHKRRFTAIRRCIDISTNRAGELKNQFEKDSSSTTLPVIIRKEQLKLRQFKSELLEEEIIQNSALKVLYERCRDHFSHPMFEKF 147
[Close]
BLAST of Coiled coil domain containing protein 58 vs. TrEMBL
Match: A0A2H1BVB8 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_14070 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.293e-33
Identity = 61/146 (41.78%), Postives = 94/146 (64.38%), Query Frame = 2 
Query:   47 DNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGP-----VDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKF 469
            D++  +PCDDF QF KLL   RK DDRI N+LN+ LPT SF+ K++ KS C  F +EM++ H  R   IK+C+    S LE + +  ++  P     V   +R++  L++QF++EL EEE+IQ +++KV Y+RCRD++     ++F
Sbjct:    4 DDDSTIPCDDFLQFTKLLGIRRKADDRIRNQLNTLLPTASFAGKVDFKSKCGDFLKEMLSYHEERNNAIKHCVSYAASRLEDLKELQANADPAEKHSVSRSLRKQQLLLRQFQSELLEEEVIQSAAVKVIYERCRDHFHHPVFERF 149
[Close]
BLAST of Coiled coil domain containing protein 58 vs. TrEMBL
Match: A0A068WSR5 (Coiled coil domain containing protein 58 OS=Echinococcus granulosus OX=6210 GN=EgrG_001142100 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.766e-33
Identity = 63/146 (43.15%), Postives = 95/146 (65.07%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCI---RNKTSYLESILKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKF 469
            M++  + +PCDDF  F KLL   RK+DDRINNELN+ALPT SFSE ++A   C  F++++   H  R   IK CI    N+ + L+   +K+ +   + + +R+E   ++QFK+E  EEEIIQ+++LKV Y+RCRD++     +KF
Sbjct:    1 MDEIADDIPCDDFLHFSKLLHAWRKSDDRINNELNAALPTASFSESVDAAKQCSLFFDKLTELHKRRFTAIKRCIDVSTNRVNDLKQRYEKDPTSTILPAAIRKEQLQLRQFKSETLEEEIIQNAALKVLYERCRDHFSSPMFEKF 146
[Close]
BLAST of Coiled coil domain containing protein 58 vs. TrEMBL
Match: A0A068YNC7 (Coiled coil domain containing protein 58 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001142100 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.826e-33
Identity = 63/146 (43.15%), Postives = 95/146 (65.07%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCI---RNKTSYLESILKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKF 469
            M++  + +PCDDF  F KLL   RK+DDRINNELN+ALPT SFSE ++A   C  F++++   H  R   IK CI    N+ + L+   +K+ +   + + +R+E   ++QFK+E  EEEIIQ+++LKV Y+RCRD++     +KF
Sbjct:    1 MDEIADDIPCDDFLHFSKLLHAWRKSDDRINNELNAALPTASFSESVDAAKQCSLFFDKLTELHKRRFTAIKRCIDVSTNRVNDLKQRYEKDPTSTILPASIRKEQLQLRQFKSETLEEEIIQNAALKVLYERCRDHFSSPMFEKF 146
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Cavefish
Match: ccdc58 (coiled-coil domain containing 58 [Source:NCBI gene;Acc:103044826])

HSP 1 Score: 101.679 bits (252), Expect = 9.279e-28
Identity = 49/140 (35.00%), Postives = 83/140 (59.29%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDG---PVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            M      L C+DF  F++ L   R  DDRI + LN+ +PT SFS K++A   C++ YE ++ +HL R+K IK CI   ++ +  + ++ + D     +  ++R+E   +K   +EL  EE+++D +LKVF +RCR ++ P
Sbjct:    1 MAAPGGTLNCEDFSMFQETLKVMRTIDDRIVHALNTTVPTASFSGKVDASQTCKELYESLLEAHLSRDKAIKACITETSAVVGQLREERAKDRDNLSIIKQLRKEQTKLKLMSSELNVEEVVKDRTLKVFVERCRIHFTP 140
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Cavefish
Match: ccdc58 (coiled-coil domain containing 58 [Source:NCBI gene;Acc:103044826])

HSP 1 Score: 88.1965 bits (217), Expect = 1.256e-22
Identity = 45/134 (33.58%), Postives = 76/134 (56.72%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDG---PVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRC 433
            M      L C+DF  F++ L   R  DDRI + LN+ +PT SFS K++A   C++ YE ++ +HL R+K IK CI   ++ +  + ++ + D     +  ++R+E   +K   +EL  EE+++D +LKV    C
Sbjct:    1 MAAPGGTLNCEDFSMFQETLKVMRTIDDRIVHALNTTVPTASFSGKVDASQTCKELYESLLEAHLSRDKAIKACITETSAVVGQLREERAKDRDNLSIIKQLRKEQTKLKLMSSELNVEEVVKDRTLKVSISVC 134
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Nematostella
Match: EDO38819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBS3])

HSP 1 Score: 75.0998 bits (183), Expect = 1.605e-17
Identity = 39/115 (33.91%), Postives = 69/115 (60.00%), Query Frame = 2 
Query:  119 DDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGPVDSE----VRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKP 451
            DD+I  +LN+++PT SF  ++NA+  C+  Y E++  +  RE  IK CI   ++ +  + +K   D P  +E    +R E  +++  ++EL  EE++++ SLKVF +RC   +KP
Sbjct:   72 DDKIIYKLNTSVPTASFEGEVNAEKRCRDLYGELLQGYSSRESAIKGCIHEVSNNIRDLREKREQD-PDSNEFSKKLRNEQTMLRLMQSELLVEEVVKERSLKVFNERCWKSFKP 185
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Medaka
Match: ccdc58 (coiled-coil domain containing 58 [Source:NCBI gene;Acc:101164684])

HSP 1 Score: 106.301 bits (264), Expect = 1.645e-29
Identity = 54/136 (39.71%), Postives = 83/136 (61.03%), Query Frame = 2 
Query:   62 LPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDG---PVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDI 460
            L C+DF  F+++L   R  DDRI + LNS +PT SFS K++A   C++ YE M+  HL R+K IK CI   +  +E + ++ + D     +  ++R+E   +K  K+EL  EE++ D SLKVF +RCR +Y P  +
Sbjct:    8 LNCEDFSLFQEVLKVMRTIDDRIIHALNSTVPTVSFSGKVDATQTCKQLYESMMEVHLNRDKAIKACIAQTSEVVEKMREQRAQDSENLGLIKQLRKEQTKLKLMKSELNVEEVVNDRSLKVFNERCRIHYTPPKV 143
[Close]
BLAST of Coiled coil domain containing protein 58 vs. Planmine SMEST
Match: SMESG000014824.1 (SMESG000014824.1)

HSP 1 Score: 293.508 bits (750), Expect = 1.672e-103
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 2 
Query:   41 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKFE 472
            MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKFE
Sbjct:    1 MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAKSVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGPVDSEVRREHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKFE 144
The following BLAST results are available for this feature:
BLAST of Coiled coil domain containing protein 58 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CCDC583.277e-2938.17coiled-coil domain containing 58 [Source:HGNC Symb... [more]
CCDC582.693e-2737.78coiled-coil domain containing 58 [Source:HGNC Symb... [more]
CCDC583.174e-2637.60coiled-coil domain containing 58 [Source:HGNC Symb... [more]
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ccdc582.233e-2237.98gene:FBgn0036909 transcript:FBtr0074880[more]
Ccdc589.167e-1938.26gene:FBgn0036909 transcript:FBtr0074879[more]
Ccdc589.167e-1938.26gene:FBgn0036909 transcript:FBtr0344256[more]
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc581.014e-3040.69coiled-coil domain containing 58 [Source:NCBI gene... [more]
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ccdc584.779e-3038.93coiled-coil domain containing 58 [Source:MGI Symbo... [more]
Ccdc581.938e-2636.62coiled-coil domain containing 58 [Source:MGI Symbo... [more]
Ccdc585.418e-1334.83coiled-coil domain containing 58 [Source:MGI Symbo... [more]
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BLAST of Coiled coil domain containing protein 58 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6PBK1|CCD58_DANRE7.655e-3040.69Coiled-coil domain-containing protein 58 OS=Danio ... [more]
sp|Q8R3Q6|CCD58_MOUSE3.343e-2938.93Coiled-coil domain-containing protein 58 OS=Mus mu... [more]
sp|B5X9S3|CCD58_SALSA8.216e-2936.11Coiled-coil domain-containing protein 58 OS=Salmo ... [more]
sp|Q4VC31|CCD58_HUMAN1.574e-2838.17Coiled-coil domain-containing protein 58 OS=Homo s... [more]
sp|A4FUI1|CCD58_BOVIN1.933e-2838.93Coiled-coil domain-containing protein 58 OS=Bos ta... [more]
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BLAST of Coiled coil domain containing protein 58 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3UM791.932e-3545.32Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A564YH003.293e-3546.76Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A2H1BVB81.293e-3341.78Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A068WSR52.766e-3343.15Coiled coil domain containing protein 58 OS=Echino... [more]
A0A068YNC72.826e-3343.15Coiled coil domain containing protein 58 OS=Echino... [more]
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ccdc589.279e-2835.00coiled-coil domain containing 58 [Source:NCBI gene... [more]
ccdc581.256e-2233.58coiled-coil domain containing 58 [Source:NCBI gene... [more]
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO388191.605e-1733.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Coiled coil domain containing protein 58 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc581.645e-2939.71coiled-coil domain containing 58 [Source:NCBI gene... [more]
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BLAST of Coiled coil domain containing protein 58 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000014824.11.672e-103100.00SMESG000014824.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007465 ID=SMED30007465|Name=Coiled coil domain containing protein 58|organism=Schmidtea mediterranea sexual|type=transcript|length=505bp
ATGTTTTGTTTAAATATTCACTAATTTGATAATTCTCGATATGAATGACA
ATAATGAGCTCTTGCCATGCGATGATTTCTTTCAATTTAAAAAACTGCTA
ACAAATCACAGAAAAAATGATGATCGAATCAACAATGAATTAAACAGTGC
TTTGCCTACTCAGTCTTTTAGTGAAAAGATAAATGCAAAGTCCGTATGTC
AAAAATTCTATGAAGAAATGATAACTTCTCATCTGTATCGCGAGAAAACC
ATTAAAAATTGTATTAGAAACAAAACATCTTATTTGGAATCCATTTTAAA
GAAAAATTCATCTGATGGTCCAGTTGACAGTGAAGTCAGGCGAGAGCATA
ATTTAGTGAAACAGTTTAAAGCAGAACTTTACGAGGAAGAAATTATTCAG
GATTCTTCATTAAAAGTTTTCTACCAGCGCTGTCGAGATTATTACAAACC
GAAAGATATCAAAAAGTTCGAATGAAATAATTTTATATTTAGGATTTCTA
GGATG
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protein sequence of SMED30007465-orf-1

>SMED30007465-orf-1 ID=SMED30007465-orf-1|Name=SMED30007465-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp
MNDNNELLPCDDFFQFKKLLTNHRKNDDRINNELNSALPTQSFSEKINAK
SVCQKFYEEMITSHLYREKTIKNCIRNKTSYLESILKKNSSDGPVDSEVR
REHNLVKQFKAELYEEEIIQDSSLKVFYQRCRDYYKPKDIKKFE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000429neoblast
PLANA:0002056medial muscle cell
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR019171Caffeine_induced_death_Cid2
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019171Caffeine-induced death protein 2PFAMPF09774Cid2coord: 10..132
e-value: 4.1E-10
score: 40.3
IPR019171Caffeine-induced death protein 2PANTHERPTHR31905FAMILY NOT NAMEDcoord: 1..138