MFS domain-containing protein

Overview
NameMFS domain-containing protein
Smed IDSMED30007407
Length (bp)1656
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MFS domain-containing protein (SMED30007407) t-SNE clustered cells

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30007407) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MFS domain-containing protein (SMED30007407) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30007407) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007407

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30007407h1SMcG0002133 dd_Smed_v4_14310_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007407h1SMcG0002133 dd_Smed_v4_14310_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30007407h1SMcG0002133 dd_Smed_v4_14310_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A419Q2S4 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_11621s PE=4 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.535e-37
Identity = 120/457 (26.26%), Postives = 216/457 (47.26%), Query Frame = 3
Query:  111 ITIFCAVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCA-IIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXXXXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEV-----PHAPEIRNFFFNPETWNVLRESSSI-IWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFL 1460
            + +F +    I I GK R FG+ +A  H     S+ +  S+S LN+IGD   ++     PF T  ++RL  P    R ++  A + I   C+++  +  P  L +T +I+ GIG+++++   ++   + F + +++HVL +AF   GP+G ++  PLF   +    WQ+ ++F  IL +                    T+    +F            D Q  S  E+   E  E+  +       +   + K+PS+A+ +     P A + R + F   + + LR +  + +W  ER LH +++Y +++NL  Y+   +   L + + + + FG GE I F I   +GD++R  L  +Y I  G   +  +   I Q  + D       + L+ + G  +   + + YA AE+V+   G IA+P+T+    +G+I AP  SG VI    Y+ FF+
Sbjct:   38 VVLFVSAVNVIFIGGKRRAFGIFVAALH-----SSYNETSMSELNWIGDSYASLGFFLMPFATTAIVRLNRPY---RFTMFLAGVTIFTSCMTSASVPDPGYLFLTHTILHGIGSTLVLCGCSLVTGDYFDKTHRFHVLATAFVSGGPYGVLLFGPLFSYWIHDYSWQEAFVFSGILFLFV------------------TWLGAVTFLPR---------DMQEYSRAELELAEPLEDRDA----GKKDELRKPKKPSVATNIYLHDQPKASDGRGWAFC--SVDHLRNNPQVFLWAFERLLHNVVIYGLLMNLTAYVTQSLNNELIRGAKVNLYFGIGESIIFTIGALIGDRIRGKLPIVYLIGAGFSALFLI---IVQRSYTDI---NVVYVLSGLTGGAVGVGNTFLYATAEEVMLVHGSIAFPMTKMVAGVGMIIAPAFSGSVIDGYGYRGFFI 447          
BLAST of MFS domain-containing protein vs. TrEMBL
Match: H2KPP0 (Monocarboxylate transporter 9 OS=Clonorchis sinensis OX=79923 GN=CLF_102350 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 3.488e-37
Identity = 115/457 (25.16%), Postives = 212/457 (46.39%), Query Frame = 3
Query:  111 ITIFCAVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCAIIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXX-XXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEV-----PHAPEIRNFFFNPETWNVLRESSSI-IWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFL 1460
            + +F +    I I GK R FG+ +A  H     S+ +  S+S LN+IGD   ++     PF T  ++RL  P     I     + I   C+++  +  P  L +T +I+ GIG+++++   ++   + F + +++HVL +AF   GP+G ++  PLF   +    WQ+ ++F  IL +    +      P++  + + +   + +      + K                                DE  +  K+PS+A+ +     P A + R + F   + + LR +  + +W  ER LH +++Y +++NL  Y+   +   L + + + + FG GE I F I   +GD++R  L  +Y I  G   +  +   I Q  + D       + L+ + G  +   + + YA AE+V+   G IA+P+T+    +G+I AP  SG VI    Y+ FF+
Sbjct:   38 VVLFVSAVNVIFIGGKRRAFGIFVAALH-----SSYNETSMSELNWIGDSYASLGFFLMPFATTAIVRLNRPYR--FIMFLAGVTIFTSCMTSASVPDPGYLFLTHTILHGIGSTLVLCGCSLVTGDYFDKTHRFHVLATAFVSGGPYGVLLFGPLFSYWIHDYSWQEAFVFSGILFLFVTWLGAVTFLPRDMQEYSRAELELAEPLADRDAGK-------------------------------KDELRKP-KKPSVATNIYLHDQPKASDGRGWAFC--SVDHLRNNPQVFLWAFERLLHNVVIYGLLMNLTAYVTQSLNNELIRGAKVNLYFGIGESIIFTIGALIGDRIRGKLPIVYLIGAGFSALFLI---IVQRSYTDI---NVVYVLSGLTGGAVGVGNTFLYATAEEVMLVHGSIAFPMTKMVAGVGMIIAPAFSGSVIDGYGYRGFFI 447          
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A4S2MEA9 (MFS domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000598 PE=4 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 4.532e-36
Identity = 114/452 (25.22%), Postives = 208/452 (46.02%), Query Frame = 3
Query:  111 ITIFCAVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCA-IIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXX-XXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFL 1460
            + +F +    I I GK R FG+ +A  H     S+ +  S+S LN+IGD   ++     PF T  ++RL  P    R ++  A + I   C+++  +  P  L +T +I+ GIG+++++   ++   + F + +++HVL +AF   GP+G ++  PLF   +    WQ+ ++F  IL +    +      P++  + + +   + +        K      +Q K    V  ++             D+      R      V H   +RN   NP+ +         +W  ER LH +++Y +++NL  Y+   +   L + + + + FG GE I F I   +GD++R  L  +Y I      +  +   I Q  + D       + L+ + G  +   + + YA AE+V+   G IA+P+T+    +G+I AP  SG VI    Y+ FF+
Sbjct:   38 VVLFVSAINVIFIGGKRRAFGIFVAALH-----SSYNETSMSELNWIGDSYASLGFFLMPFATTAIVRLNRPY---RFTMFLAGVTIFTSCMTSASVPDPGYLFLTHTILHGIGSTLVLCGCSLVTGDYFDKTHRFHVLATAFVSGGPYGVLLFGPLFSYWIHDYSWQEAFIFSGILFLFVTWLGAVTFLPRDMHEYSRAELTLSEPLAERDVGK--KDSLKQSKKQSVVANVDL-----------RDQPKASDGRGWAFCSVDH---LRN---NPQVF---------LWAFERLLHNVVVYGLLMNLTAYVTQSLNNELTRGAKVNLYFGIGESIIFTIGALIGDRIRGKLPLVYLIGAAFSALFLI---IVQRSYTDI---NVVYVLSGLTGGAVGVGNTFLYATAEEVMLVHGSIAFPMTKMVAGVGMIIAPAFSGSVIDGYGYRGFFI 447          
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A5J4NWZ5 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0013706 PE=4 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 9.311e-36
Identity = 115/443 (25.96%), Postives = 205/443 (46.28%), Query Frame = 3
Query:  141 IIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCA-IIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXXXXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPS--IASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFL 1460
            I I GK R FG+ +A  H     +A +  S++ LN+IGD   ++     PF T  +I L  P    R+++  A +II + C+++  +  P  L +T +I+ GIG+++I+   ++   + F + ++YHVL +AF   GP+G +I  PLF   +    WQQ +++  IL ++   +               TF     F                  Y E  +    EN+    +V   E   +  RP+  +   +P A + R + F     ++      ++W  ER L  +++Y +++NL  Y+   +   + + + + + FG GE   F +   +GD++R HL  +Y I      +      I Q  + D       + LA   G  +   + + YA AE+V+   G IA+P+T+    +G++ AP+ SG VI    Y+ FF+
Sbjct:   48 ICIGGKRRAFGIFIAALH-----TAYNETSMTELNWIGDSYASLGFFLMPFATTAIITLNRPY---RMTMFLAGLIIFVSCMTSAAVPDPGYLFLTHTILHGIGSTLILCGTSLVTGDYFDKTHRYHVLATAFVSGGPYGVLIFGPLFSYWIHDYSWQQAFVYSGILFLLTTWL------------GAITFLPRYMF-----------------DYMEAVDGNGSENEM---AVKKPETKPDNSRPTPILLRNLPKASDGRGWAFC-SVEHIRHNPQIVLWSFERLLQNVVVYGLLMNLTSYVSEALNNEIIRGAKVNLYFGIGESFVFTVGALIGDRIRGHLPLVYLIGAAFAALFLF---IVQRSYTDI---NVIYLLAGFTGAAVGVGNTFLYATAEEVMLVHGSIAFPMTKMVAGVGMLIAPVFSGGVIDKFGYRGFFI 443          
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1S8WM16 (Transporter, major facilitator family protein OS=Opisthorchis viverrini OX=6198 GN=X801_08712 PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 1.553e-35
Identity = 117/454 (25.77%), Postives = 212/454 (46.70%), Query Frame = 3
Query:  111 ITIFCAVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCA-IIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXX-XXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPD--HPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFL 1460
            + +F +    I I GK R FG+ +A  H     S+ +  S+S LN+IGD   ++     PF T  ++RL  P    R ++  A + I   C+++  +  P  L +T +I+ GIG+++++   ++   + F + +++HVL +AF   GP+G ++  PLF   +    WQ  ++F  IL +    +   I  P++  + + +   + +      + K  D   P +Q  +              +IY     +AS+   R      V H   +R    NP+ +         +W  ER LH +++Y +++NL  Y+   +   L + + + + FG GE I F I   +GD++R  L  +Y I      +  +   I Q  + D       + L+ + G  +   + + YA AE+V+   G IA+P+T+    +G+I AP  SG VI    Y+ FF+
Sbjct:   38 VVLFVSAINVIFIGGKRRAFGIFVAALH-----SSYNETSMSELNWIGDSYASLGFFLMPFATTAIVRLNRPY---RFTMFLAGVTIFTSCMTSASVPDPSYLFLTHTILHGIGSTLVLCGCSLVTGDYFDKTHRFHVLATAFVSGGPYGVLLFGPLFSYWIHDYSWQDAFIFSGILFLFVTWLGAVIFLPRDMHEYSRAELTLSEPLADRDAGKKDDMRKPKKQSAA-------------ANIYLRDQPKASD--GRGWAFCSVDH---LRT---NPQVF---------LWAFERLLHNIVIYGLLMNLTAYVTQSLNNELIRGAKVNLYFGIGESIIFTIGALIGDRIRGKLPIVYLIGAAFSALFLI---IVQRSYTDI---NVVYVLSGLTGGAVGVGNTFLYATAEEVMLVHGSIAFPMTKMVAGVGMIIAPAFSGSVIDGYGYRGFFI 447          
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000065239.1 (SMESG000065239.1)

HSP 1 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 3
Query:  567 MCPLFGVILQTTHWQQVYLFFXXXXXXXXXXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFLIQXXXXXXXXXXXXXXXXXXXQNKLVHSESCLPK 1562
            MCPLFGVILQTTHWQQVYLFFAILLVIAIIITSIVEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFLIQTILTFIRLVLLIVLIFKLKQNKLVHSESCLPK
Sbjct:    1 MCPLFGVILQTTHWQQVYLFFAILLVIAIIITSIVEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFLIQTILTFIRLVLLIVLIFKLKQNKLVHSESCLPK 332          
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000014825.1 (SMESG000014825.1)

HSP 1 Score: 259.61 bits (662), Expect = 3.726e-80
Identity = 152/463 (32.83%), Postives = 263/463 (56.80%), Query Frame = 3
Query:   90 FTNLR--KHITIFC-AVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCAIIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXXXXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQA-IHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFLIQ 1466
            F +L+  K I+I C  + + ++++GK RCF V+ A   N+TC +    C +++LN+I  L   I + G+PF+T   IR+ +  LGG+++ Q  +I+ LG L T F+  P+SL VTD++IQGI TSV++ +++ +L  ++P +N  H  V   ++  PW  VI+CPL+G ++    WQ  YL  + L+++  ++  I+EPK      VS          E   +  D  +   ++ EE++   ++  D+S Y    D    E + PS+               N     V+ +S  ++W IERAL  +  +N+++NLV YM   + E + +AS+IM++ G G ++GF+    LG ++R +L  IY +A+ +L + F+ W I  +   +  F+  DAHF+ ++ G   A ++  +Y +AED++ RP II+  LT+  Q+LGL+  P++SG++IQ D + SFF +Q
Sbjct:   18 FASLKSLKVISIVCLTISLCVVLNGKTRCFSVLAAELQNQTCNTIKDTCGMATLNWIAFLSEMIIVGGNPFITNMFIRVHSGVLGGKVTFQFCLILTLGTLLTGFVGDPESLFVTDTLIQGISTSVLLGLVSFKLVHMYPDDNPNHKTVLLLSEIAPWFGVILCPLYGTLVSVYGWQSSYLLISFLIMVICVLAGIIEPK------VSA--------TEEQEENEDSDNSSIQNTEEIDSQTYF--DKSDYWEIEDVGEMECQHPSLL----------QLLLNVNISQVVGKSEVVVWFIERALMIIAEFNLIINLVHYMTSVLLEPILQASMIMITLGAGNIMGFLTIYVLGSKIRKYLPRIYILASVMLVISFIVWQIYGSQTDLILFTVPDAHFVVIVIGAFSAISTAQSYYVAEDIIERPRIISVSLTKLAQSLGLLIGPISSGMLIQIDFFHSFFSVQ 454          
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000011418.1 (SMESG000011418.1)

HSP 1 Score: 125.946 bits (315), Expect = 2.831e-31
Identity = 115/458 (25.11%), Postives = 200/458 (43.67%), Query Frame = 3
Query:  105 KHITIFCAVFISI---IISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCAIIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXXXXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKSYEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNPETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYM--VVEIQESLEKASLIMMSFGGGEVIGFVIAVGLGDQLRHHL--LHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFF 1457
            K ++IF  V  +I    I GK R FG+ ++ F      S  +  S+  LN++GD   A+A    PF+T F+ R ++   G    +  +I++   C+ T+F+     L +T +++ GIG++ I+S  A+ + E F + ++YHVL ++ T  GP+G++I  PL+G ++Q   W+  +                       +     F +E S    +    PD        Y++  EIE +E                             P ++    N + +         +W I+R   + ILY +++NL D +  ++   E+LE  + I +  G GE I F I+  +GD++R  L   H+   A  +L +I M         I +FS        +  G      +   YA +E++    G I+ PL+    A G+I AP+ SGL+I    +   F
Sbjct:   15 KQLSIFILVVATINIIFIGGKRRTFGIFISAFR-----STFNGTSMVELNWVGDSYSAVAFFLMPFITSFVHRNRSYQKG---MLAASILVFCSCMLTSFVTNAAELFITHTVLHGIGSAFILSTSALIIGEYFDKNHRYHVLATSLTTGGPYGSLIFGPLYGFMVQHYDWRFTF-----------------------RVIAILFFVEISAGILAYVPKPD--------YQKSEEIENYEGA--------------------------FPSLKYLIQNKQIF---------LWGIDRICTSSILYGLLMNLTDLLRSMLNGTENLEGGAKINLMMGVGESIIFTISAVIGDKIRGKLPFFHLIGAAFTLLMLILMKTVSYYPYAIIAFS--------IFLGAGAGVGNCMLYASSEEITFLHGSISLPLSNMMMAFGMIIAPIFSGLLIDMYGFLGLF 390          
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000046854.1 (SMESG000046854.1)

HSP 1 Score: 75.0998 bits (183), Expect = 4.366e-14
Identity = 46/185 (24.86%), Postives = 88/185 (47.57%), Query Frame = 3
Query:   69 PWIQSQNFTNLRKHITIFCAVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCAIIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYL 623
            PWI           I +F A+ + I + GK R FG+ +A  HN    +     S++ LN+IGD   A+    +   T  +IR        +    C I+  + CL++ ++     L +T ++  GIG+S+I+SV+ + + E F   +KYH+L +     G   +++    +  ++    W++ ++
Sbjct:   36 PWI-----------ILLFSALNV-IFLGGKRRAFGIFIAELHNGVFNTT----SLAELNWIGDSYAALGYLTTTISTAVIIRFHRKYRVFQFIGACCIL--MACLASAYVTSAHWLFLTHTVFHGIGSSLILSVVGLVVGEHFDLNHKYHILATTLVSGGSLASIVFVQFYAYLIDQYGWRKAFV 202          

HSP 2 Score: 57.3806 bits (137), Expect = 1.594e-8
Identity = 41/171 (23.98%), Postives = 84/171 (49.12%), Query Frame = 3
Query:  966 IIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGE----VIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFLIQ 1466
            I+W ++R + +++ Y ++LNL DY+           SL M+ F  GE    + G +++          L ++  I++ ++ +   +W I  +    +F+   +  L+   G  +  +  + +   E++   PG IAYP +     LG++ +P+ S ++ Q  +YK FFL+Q
Sbjct:  252 ILWTLDRIVTSIVTYGMLLNLADYLRRNSSSLNTSTSLTML-FAAGEATTYIQGAIVSALTKKWFASKLKYLLLISSLLMVICLFAWEILAS----NFTW--SCILSYCSGFCLGPSITFLFPAGEELTTLPGEIAYPFSLAGMGLGMLLSPVLSAVIAQKYKYKWFFLVQ 415          
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000026519.1 (SMESG000026519.1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.670e-13
Identity = 53/233 (22.75%), Postives = 104/233 (44.64%), Query Frame = 3
Query:   63 KWPWIQSQNFTNLRKHITIFCAVFISIIISGKGRCFGVVLAHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPALGGRISVQCAIIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIRLTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFXXXXXXXXXXXXXXEPKNYDQPAVSTFRIEKSFKAESSSKCPDH 761
            K PW    N  ++   + +F A    I + GK R FG  +A              +++ LN+IGD   A+    S      ++  +     G      AI +++ C++++F+  P  L +T ++  GIG+S+I+S + + + + F  ++ YH+L +     G   ++I   LF  ++Q+  W+  ++    L  I     ++   K+ +Q       +E + K   S +C  H
Sbjct:    7 KLPW----NRASVLPWVILFFAALNVIFLGGKRRAFGSFVAEMRRD-----YPNITLTELNWIGDSYAAVGYMTSGISIAIILFFKRRY--GMFQFMGAIWVLVSCVTSSFVPNPHWLFLTHTLFHGIGSSLIISTVGLVVNDHFDSKHPYHILATTLVSGGSVASIIFVTLFASLIQSYGWRYAFIILGCLYFIVNASAALFFRKSNNQ-------VEDTNKKTVSCRCEGH 221          

HSP 2 Score: 57.7658 bits (138), Expect = 1.139e-8
Identity = 36/170 (21.18%), Postives = 81/170 (47.65%), Query Frame = 3
Query:  969 IWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLIMMSFGGGEVIGFVIAVGLG----DQLRHHLLHIYTIATGILTVIFMSWHITQAIHIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFCQALGLIFAPLTSGLVIQFDQYKSFFLIQ 1466
            +W I+R + +++ Y ++LNL DYM    +++L +++ +   F  GE I ++I   +     D  +  L +I    +  +++  ++    + I          + L  + G  +  +  + +   E++   PG +AYP++      G++ +PL +  + +   YK FF +Q
Sbjct:  232 LWFIDRVMTSVVTYGLLLNLTDYMY-RTEKNLVRSTTLTTLFASGEAITYLIGATITAITRDFFKDKLRYILLGTSFAMSICLIALEYFKGII------SVCYILCFLAGFTLGPSITFLFPAGEELTRLPGHMAYPISLAGMGFGMLVSPLLTAKIAEIFLYKWFFFVQ 394          
The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A419Q2S41.535e-3726.26Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
H2KPP03.488e-3725.16Monocarboxylate transporter 9 OS=Clonorchis sinens... [more]
A0A4S2MEA94.532e-3625.22MFS domain-containing protein OS=Opisthorchis feli... [more]
A0A5J4NWZ59.311e-3625.96Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A1S8WM161.553e-3525.77Transporter, major facilitator family protein OS=O... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000065239.10.000e+0100.00SMESG000065239.1[more]
SMESG000014825.13.726e-8032.83SMESG000014825.1[more]
SMESG000011418.12.831e-3125.11SMESG000011418.1[more]
SMESG000046854.14.366e-1424.86SMESG000046854.1[more]
SMESG000026519.11.670e-1322.75SMESG000026519.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007407 ID=SMED30007407|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1656bp
GAAAATACTTGACACATTTTTCTAGTTGAAAAATGAAAGTTCTTGAGTAT
CTGTCAACGTTGAAATGGCCGTGGATACAATCGCAAAATTTCACGAATCT
AAGAAAACACATTACGATATTCTGTGCTGTATTTATCAGTATAATCATAT
CGGGTAAAGGTAGATGCTTCGGAGTGGTGTTGGCGCATTTTCATAATAAG
ACTTGTACATCTGCCGCACATAGATGCTCGATCTCTTCGCTTAATTTCAT
TGGAGATTTAGGCGTTGCAATAGCTTTGGCTGGTAGTCCCTTTGTCACTG
GATTTTTAATCAGACTTCAAACTCCAGCTTTAGGAGGACGGATTTCAGTT
CAATGTGCGATAATTATTATTTTAGGTTGTTTATCGACGAATTTCATTAT
TCAACCAGACTCTCTTATTGTTACGGATTCGATTATCCAAGGAATCGGAA
CATCAGTTATAGTATCTGTTATTGCTATTCGACTTACCGAAATATTCCCC
AGGGAGAATAAGTATCATGTACTGGTTAGTGCATTCACTGATGCAGGGCC
ATGGGGAACGGTTATTATGTGTCCTCTTTTCGGAGTAATTCTACAAACAA
CACATTGGCAACAAGTCTACCTATTTTTTGCGATTCTTTTAGTAATTGCA
ATCATTATTACTAGCATAGTTGAACCGAAAAATTACGATCAACCTGCAGT
TTCAACGTTTCGAATAGAAAAATCCTTTAAAGCAGAATCATCATCGAAGT
GTCCAGATCATCCAGATCAACAGTGCAAATCATATGAAGAAGTAAATGAA
ATAGAATTTTGGGAAAATGACCAATCAATTTATTCCGTAAGTTCTGATGA
AGCTAGTGAAGAAGTAAAAAGACCTTCAATTGCATCTGAAGTCCCACACG
CCCCAGAAATAAGAAATTTCTTTTTTAATCCCGAAACTTGGAATGTTCTC
CGAGAAAGTTCATCGATTATATGGTTGATTGAGAGAGCTCTACATACAAT
GATTTTGTACAATGTCGTGTTGAATTTGGTTGACTACATGGTAGTGGAAA
TTCAAGAAAGTCTGGAAAAGGCTTCGTTAATAATGATGTCCTTTGGTGGT
GGAGAGGTAATTGGCTTTGTAATTGCTGTTGGTCTGGGTGATCAATTACG
TCACCACCTTTTACATATTTATACTATAGCCACTGGAATTTTGACTGTGA
TATTCATGAGTTGGCACATTACTCAGGCCATTCATATTGATTCATTTTCA
AATGGAGATGCTCACTTTTTGGCATTGATATGTGGTCTTGTAATGGCTTT
CACTAGTGGATATACATATGCTCTCGCTGAAGACGTTTTATGTCGACCAG
GAATAATTGCTTACCCTCTCACAAGATTTTGCCAAGCTTTAGGGCTTATT
TTTGCTCCTCTTACATCTGGTTTAGTCATACAGTTTGATCAATACAAATC
TTTCTTCCTGATTCAAACAATTCTAACCTTTATAAGACTAGTTCTCCTCA
TAGTACTGATATTCAAACTCAAACAAAATAAATTAGTTCATAGTGAATCA
TGTCTGCCGAAATGATACTTAAACATGAAAATGAATGGAAAATAGTCGAT
GGTATTAGGACTTATTTATAAAAATAGAATTTATTGATACGAATGTTCAT
GGAGAG
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protein sequence of SMED30007407-orf-1

>SMED30007407-orf-1 ID=SMED30007407-orf-1|Name=SMED30007407-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=511bp
MKVLEYLSTLKWPWIQSQNFTNLRKHITIFCAVFISIIISGKGRCFGVVL
AHFHNKTCTSAAHRCSISSLNFIGDLGVAIALAGSPFVTGFLIRLQTPAL
GGRISVQCAIIIILGCLSTNFIIQPDSLIVTDSIIQGIGTSVIVSVIAIR
LTEIFPRENKYHVLVSAFTDAGPWGTVIMCPLFGVILQTTHWQQVYLFFA
ILLVIAIIITSIVEPKNYDQPAVSTFRIEKSFKAESSSKCPDHPDQQCKS
YEEVNEIEFWENDQSIYSVSSDEASEEVKRPSIASEVPHAPEIRNFFFNP
ETWNVLRESSSIIWLIERALHTMILYNVVLNLVDYMVVEIQESLEKASLI
MMSFGGGEVIGFVIAVGLGDQLRHHLLHIYTIATGILTVIFMSWHITQAI
HIDSFSNGDAHFLALICGLVMAFTSGYTYALAEDVLCRPGIIAYPLTRFC
QALGLIFAPLTSGLVIQFDQYKSFFLIQTILTFIRLVLLIVLIFKLKQNK
LVHSESCLPK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
GO:0015667site-specific DNA-methyltransferase (cytosine-N4-specific) activity
GO:0003677DNA binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
IPR017985MeTrfase_CN4_CS
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
GO:0090124N-4 methylation of cytosine
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 1..313
NoneNo IPR availableCDDcd17352MFS_MCT_SLC16coord: 127..314
e-value: 9.95235E-4
score: 38.3025
NoneNo IPR availableTMHMMTMhelixcoord: 266..288
NoneNo IPR availableTMHMMTMhelixcoord: 232..254
NoneNo IPR availableTMHMMTMhelixcoord: 298..316
NoneNo IPR availableTMHMMTMhelixcoord: 13..35
NoneNo IPR availableTMHMMTMhelixcoord: 198..217
NoneNo IPR availableTMHMMTMhelixcoord: 169..191
NoneNo IPR availableTMHMMTMhelixcoord: 137..159
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 3..322