SMEDWI-1
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Expression Details SMEDWI-1 ( piwi-1 ) is expressed during Stage 2, Stage 3, Stage 4, Stage 5, Stage 6, Stage 7 and Stage 8 in the neoblast and blastomere
Click to see image symbols and abbreviations
piwi-1 is expressed in all undifferentiated blastomeres of S3 embryos. piwi-1 is expressed in all undifferentiated blastomeres of S3 embryos. SPIM reconstructed S3 embryo costained with piwi-1 (red) and EF1a-like-1 (green). piwi-1 is expressed in all undifferentiated blastomeres in the embryonic wall (piwi-1+, EF1a-like-1+ cells). piwi-1 is not expressed in differentiated tissues marked by EF1a-like-1 alone, including the primitive ectoderm and temporary embryonic pharynx (green). Several fluorescent beads used for 3-dimensional reconstruction are visible (red).
Cell cycle activity is restricted to piwi-1 blastomeres, and all blastomeres are cycling. SPIM reconstructed S4 embryo costained with piwi-1 (red) and PCNA (green). PCNA expression is restricted to piwi-1+ blastomeres, and all piwi-1+ cells co-express PCNA.
Cell cycle activity is restricted to piwi-1 blastomeres, and all blastomeres are cycling. SPIM reconstructed S4 embryo costained with piwi-1 (red) and RNR M2-2 (green). RNR M2-2 expression is restricted to piwi-1+ blastomeres, and all piwi-1+ cells co-express RNR M2-2. Several fluorescent beads used for 3-dimensional reconstruction are visible (red).
Mitotic activity is restricted to piwi-1+ blastomeres, which cycle asynchronously. SPIM reconstructed S4 embryo constained with piwi-1 and LYAG-like (both in green) and H3S10p antibodies (red). LYAG-like marks the temporary embryonic pharynx and is not expressed in piwi-1+ blastomeres. Several examples of piwi-1+, H3S10p+ cells are evident.
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-2. SPIM reconstructed S4 embryo costained with piwi-1 (red) and piwi-2 (green). piwi-1+ blastomeres co-express the nuage factor piwi-2, and virtually all piwi-2+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (green).
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-3. SPIM reconstructed S4 embryo costained with piwi-1 (red) and piwi-3 (green). piwi-1+ blastomeres co-express the nuage factor piwi-3, and virtually all piwi-3+ cells co-express piwi-1.
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene tud-1. SPIM reconstructed S4 embryo costained with piwi-1 (red) and tud-1 (green). piwi-1+ blastomeres co-express the nuage factor tud-1, and virtually all tud-1+ cells co-express piwi-1.
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene bruli-1. SPIM reconstructed S4 embryo costained with piwi-1 (red) and bruli-1(green). piwi-1+ blastomeres co-express the stem cell maintenance gene bruli-1, and virtually all bruli-1+ cells co-express piwi-1. Several fluorescent beads used 3-dimensional reconstruction are visible (red).
piwi-1+ blastomeres possess chromatoid bodies. SPIM reconstructed S4 embryo costained with piwi-1 (red) and Y12 antibodies (green). Y12 antibody staining was restricted to, and present throughout, the piwi-1+ blastomere population.
Definitive epidermal progenitors arise in the piwi-1+ blastomere population during S5. SPIM reconstructed S5 embryo costained with piwi-1 (red) and p53 (green). Definitive epidermal progenitors, coexpressing piwi-1 and p53, are dispersed in the embryonic wall. As definitive epidermal progenitors differentiate, they are predicted to downregulate piwi-1 and retain expression of p53.
Definitive gut progenitors arise in the piwi-1+ blastomere population during S5. SPIM reconstructed S5 embryo costained with piwi-1 and VAL-like (both in red) and gata456a (green). Definitive gut progenitors, coexpressing piwi-1 and gata456a, are dispersed in the embryonic wall. As definitive gut progenitors differentiate, they are predicted to downregulate piwi-1 and retain expression of gata456a. VAL-like is expressed the temporary embryonic pharynx (at bottom) and is not detected in piwi-1+ blastomeres
Muscle progenitors arise in the piwi-1+ blastomere population during S5. SPIM reconstructed S5 embryo costained with piwi-1 and VAL-like (both in red) and myoD (green). Muscle progenitors, coexpressing piwi-1 and myoD, are dispersed in the embryonic wall. As muscle progenitors differentiate, they are predicted to downregulate piwi-1 and retain expression of myoD. VAL-like is expressed the temporary embryonic pharynx (at bottom) and is not detected in piwi-1+ blastomeres
Neural progenitors arise in the piwi-1+ blastomere population during S5. SPIM reconstructed S5 embryo costained with piwi-1 and VAL-like (both in red) and pax6a (green). Neural progenitors, coexpressing piwi-1 and pax6a, are dispersed in the embryonic wall. As neural progenitors differentiate, they are predicted to downregulate piwi-1 and retain expression of pax6a. VAL-like is expressed the temporary embryonic pharynx (at bottom) and is not detected in piwi-1+ blastomeres
Cell cycle activity is restricted to piwi-1 blastomeres, and all blastomeres are cycling. SPIM reconstructed S3 embryo costained with piwi-1 (red) and PCNA (green). PCNA expression is restricted to piwi-1+ blastomeres, and all piwi-1+ cells co-express PCNA.
Cell cycle activity is restricted to piwi-1 blastomeres, and all blastomeres are cycling. SPIM reconstructed S5 embryo costained with piwi-1 (red) and PCNA (green). PCNA expression is restricted to piwi-1+ blastomeres, and all piwi-1+ cells co-express PCNA.
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-2. SPIM reconstructed S3 embryo costained with piwi-1 (green) and piwi-2 (red). piwi-1+ blastomeres co-express the nuage factor piwi-2, and virtually all piwi-2+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (green).
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-2. SPIM reconstructed S5 embryo costained with piwi-1 (green) and piwi-2 (red). piwi-1+ blastomeres co-express the nuage factor piwi-2, and virtually all piwi-2+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (green).
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-3. SPIM reconstructed S3 embryo costained with piwi-1 (green) and piwi-3 (red). piwi-1+ blastomeres co-express the nuage factor piwi-3, and virtually all piwi-3+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (red).
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-3. SPIM reconstructed S5 embryo costained with piwi-1 (green) and piwi-3 (red). piwi-1+ blastomeres co-express the nuage factor piwi-3, and virtually all piwi-3+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (red).
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene tud-1. SPIM reconstructed S5 embryo costained with piwi-1 (red) and tud-1 (green). piwi-1+ blastomeres co-express the nuage factor tud-1, and virtually all tud-1+ cells co-express piwi-1.
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene tud-1. SPIM reconstructed S3 embryo costained with piwi-1 (red) and tud-1 (green). piwi-1+ blastomeres co-express the nuage factor tud-1, and virtually all tud-1+ cells co-express piwi-1. Anatomical Expression
PAGE et. al., 2020SMED30007406 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 85
Homology
BLAST of SMEDWI-1 vs. Ensembl Human
Match: PIWIL1 (piwi like RNA-mediated gene silencing 1 [Source:HGNC Symbol;Acc:HGNC:9007]) HSP 1 Score: 200.29 bits (508), Expect = 2.349e-67 Identity = 143/507 (28.21%), Postives = 237/507 (46.75%), Query Frame = -2
Query: 54 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSN--EVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMD-SISSCRSNEVEDALTDFIRV------SKVHMALVFIPD---DKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1517
++ IP ELCYL G +D R++ N+ K+L + P +R ++ + + + ++ WG+ + ++ GR L I N + D K + + ++ RN N+ I+++ ++ M + EV+D ++RV + + + + DK A K +QCV R + + +A K +QM K+G + W +++ + MIVG+D +H T G+RS+ V SI+ +++ S E + L AL + + MP R+I+YRDGVGD QL +E + ++ I P++ +VVKKR++ +FF NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ D S + P +Q++TY L H+Y+NW G IRVP P YAH+LA LVG+ IHR N + RL+YL
Sbjct: 380 AMLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNL------FKVTPAMGMQMRKAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQ--QTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHR-----EPNLSLSNRLYYL 861 HSP 2 Score: 77.7962 bits (190), Expect = 2.349e-67 Identity = 72/286 (25.17%), Postives = 126/286 (44.06%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSD-----EDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV------------YRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEV---QLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G +V L +N+ + + Y Y + +ME + +R+ L + + G+ FDG LF + T N +DV I+I L S Q NI+F + M ++IGR ++ ++ P I G++T+I + N S +L + ++VL V+ ++ +LIG ++TKYNNKTYR+ DI + D N + + ++S+L Y++++YN + + KQP ++S+ KR
Sbjct: 104 KTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSAL----LFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNG--EDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPND---------PIDIPSHRLVIWPGFTTSILQYEN-----SIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKR 368
BLAST of SMEDWI-1 vs. Ensembl Human
Match: PIWIL1 (piwi like RNA-mediated gene silencing 1 [Source:HGNC Symbol;Acc:HGNC:9007]) HSP 1 Score: 200.29 bits (508), Expect = 2.349e-67 Identity = 143/507 (28.21%), Postives = 237/507 (46.75%), Query Frame = -2
Query: 54 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSN--EVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMD-SISSCRSNEVEDALTDFIRV------SKVHMALVFIPD---DKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1517
++ IP ELCYL G +D R++ N+ K+L + P +R ++ + + + ++ WG+ + ++ GR L I N + D K + + ++ RN N+ I+++ ++ M + EV+D ++RV + + + + DK A K +QCV R + + +A K +QM K+G + W +++ + MIVG+D +H T G+RS+ V SI+ +++ S E + L AL + + MP R+I+YRDGVGD QL +E + ++ I P++ +VVKKR++ +FF NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ D S + P +Q++TY L H+Y+NW G IRVP P YAH+LA LVG+ IHR N + RL+YL
Sbjct: 380 AMLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNL------FKVTPAMGMQMRKAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQ--QTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYN-PRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN----------SGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHR-----EPNLSLSNRLYYL 861 HSP 2 Score: 77.7962 bits (190), Expect = 2.349e-67 Identity = 72/286 (25.17%), Postives = 126/286 (44.06%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSD-----EDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV------------YRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEV---QLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G +V L +N+ + + Y Y + +ME + +R+ L + + G+ FDG LF + T N +DV I+I L S Q NI+F + M ++IGR ++ ++ P I G++T+I + N S +L + ++VL V+ ++ +LIG ++TKYNNKTYR+ DI + D N + + ++S+L Y++++YN + + KQP ++S+ KR
Sbjct: 104 KTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSAL----LFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNG--EDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPND---------PIDIPSHRLVIWPGFTTSILQYEN-----SIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKR 368
BLAST of SMEDWI-1 vs. Ensembl Human
Match: PIWIL3 (piwi like RNA-mediated gene silencing 3 [Source:HGNC Symbol;Acc:HGNC:18443]) HSP 1 Score: 195.667 bits (496), Expect = 2.614e-62 Identity = 152/528 (28.79%), Postives = 249/528 (47.16%), Query Frame = -2
Query: 54 SSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA---HRFGVLVV---DRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDD--KVYAKVKNFTMS-TGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF-KDGAN---PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1589
S RW T+ EP L IP +LC++ G +D + ++ K L + PR R + +++F K ++ ++ W + + +++ GR L +I + DW EI+ ++P A H + +L R ++ V +G M + +N D L + R + + M + +P+D + Y +K + + + +QCV ++ R T+ K QM K+G W + + TM VG+D FH + +S+ V S +A+ +++ S K E + L AL + ++MP +I+YRDGVGD QL E + ++ I N + ++VVKKRI+ +FF K G+N P PGTV+D ++ + +Y+F++VSQ GT +PT+YNV+ DT +SP +Q++TY L H+Y+N G IRVP P HYAH+LA LVG+ IH+ N + RLFYL
Sbjct: 270 SQGRWKKGLTGTQREP--------ILLIP-QLCHMTGLTDEICKDYSIVKELAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGRRMVKANSQGDWS-REIR-ELPLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDG-DANSYIDTLRKYTRPT-LQMVICILPNDDKRRYDSIKRYLCTKCPIPSQCVVKKTLEKVQAR---TIVTKIAQQMNCKMGGALWKVETDVQRTMFVGIDCFHDIVNRQKSIAGFVASTNAELTKWYSQCVIQKTGEELVKELEICLKAALDVWCKNESSMPHSVIVYRDGVGDGQLQALLDHEAKKMSTYLKTISPNNF--TLAFIVVKKRINTRFFLKHGSNFQNPPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDT----------IGLSPDTVQRLTYCLCHMYYNLPGIIRVPAPCHYAHKLAYLVGQSIHQ-----EPNRSLSTRLFYL 764 HSP 2 Score: 65.4698 bits (158), Expect = 2.614e-62 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = -1
Query: 1591 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVV----MENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTS-------KWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNE----NGTASTLLYLER----INRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISR 2367
K G +G VV L +N+ + + Y Y+V +E+ ++RT +L + + G+ FDG L + +W S +N+V VE S L T S + + NI+F + E++GR ++ R G S +I GY T++ + N + LL +E I R + ++ R + ++LIG ++TKYNNKTYR+ DI + + D D KI+Y++Y++Q++ + KQP ++S+
Sbjct: 8 KTGSEGTVVQLLANHFRVISRPQWVAYKYNVDYKPDIEDGNLRT----ILLDQHRRKFGERHIFDGNSLLLSRPLKERRVEWLSTTKDKNIVKITVEFSKELTPT----SPDCLRYYNILFRRTFKLLDFEQVGRNYYTKKKAIQL-YRHGTSL------EIWLGYVTSVLQYENSITLCADVSHKLLRIETAYDFIKRTSAQAQTGNI-REEVTNKLIGSIVLTKYNNKTYRVDDIDWKQNPEDTFNKSDGSKITYIDYYRQQHKEIVTVKKQPLLVSQ 271
BLAST of SMEDWI-1 vs. Ensembl Human
Match: PIWIL3 (piwi like RNA-mediated gene silencing 3 [Source:HGNC Symbol;Acc:HGNC:18443]) HSP 1 Score: 195.667 bits (496), Expect = 2.614e-62 Identity = 152/528 (28.79%), Postives = 249/528 (47.16%), Query Frame = -2
Query: 54 SSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA---HRFGVLVV---DRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDD--KVYAKVKNFTMS-TGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF-KDGAN---PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1589
S RW T+ EP L IP +LC++ G +D + ++ K L + PR R + +++F K ++ ++ W + + +++ GR L +I + DW EI+ ++P A H + +L R ++ V +G M + +N D L + R + + M + +P+D + Y +K + + + +QCV ++ R T+ K QM K+G W + + TM VG+D FH + +S+ V S +A+ +++ S K E + L AL + ++MP +I+YRDGVGD QL E + ++ I N + ++VVKKRI+ +FF K G+N P PGTV+D ++ + +Y+F++VSQ GT +PT+YNV+ DT +SP +Q++TY L H+Y+N G IRVP P HYAH+LA LVG+ IH+ N + RLFYL
Sbjct: 270 SQGRWKKGLTGTQREP--------ILLIP-QLCHMTGLTDEICKDYSIVKELAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGRRMVKANSQGDWS-REIR-ELPLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDG-DANSYIDTLRKYTRPT-LQMVICILPNDDKRRYDSIKRYLCTKCPIPSQCVVKKTLEKVQAR---TIVTKIAQQMNCKMGGALWKVETDVQRTMFVGIDCFHDIVNRQKSIAGFVASTNAELTKWYSQCVIQKTGEELVKELEICLKAALDVWCKNESSMPHSVIVYRDGVGDGQLQALLDHEAKKMSTYLKTISPNNF--TLAFIVVKKRINTRFFLKHGSNFQNPPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDT----------IGLSPDTVQRLTYCLCHMYYNLPGIIRVPAPCHYAHKLAYLVGQSIHQ-----EPNRSLSTRLFYL 764 HSP 2 Score: 65.4698 bits (158), Expect = 2.614e-62 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = -1
Query: 1591 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVV----MENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTS-------KWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNE----NGTASTLLYLER----INRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISR 2367
K G +G VV L +N+ + + Y Y+V +E+ ++RT +L + + G+ FDG L + +W S +N+V VE S L T S + + NI+F + E++GR ++ R G S +I GY T++ + N + LL +E I R + ++ R + ++LIG ++TKYNNKTYR+ DI + + D D KI+Y++Y++Q++ + KQP ++S+
Sbjct: 8 KTGSEGTVVQLLANHFRVISRPQWVAYKYNVDYKPDIEDGNLRT----ILLDQHRRKFGERHIFDGNSLLLSRPLKERRVEWLSTTKDKNIVKITVEFSKELTPT----SPDCLRYYNILFRRTFKLLDFEQVGRNYYTKKKAIQL-YRHGTSL------EIWLGYVTSVLQYENSITLCADVSHKLLRIETAYDFIKRTSAQAQTGNI-REEVTNKLIGSIVLTKYNNKTYRVDDIDWKQNPEDTFNKSDGSKITYIDYYRQQHKEIVTVKKQPLLVSQ 271
BLAST of SMEDWI-1 vs. Ensembl Human
Match: PIWIL3 (piwi like RNA-mediated gene silencing 3 [Source:HGNC Symbol;Acc:HGNC:18443]) HSP 1 Score: 190.274 bits (482), Expect = 1.648e-60 Identity = 154/536 (28.73%), Postives = 250/536 (46.64%), Query Frame = -2
Query: 54 SSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA---HRFGVLVV---DRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVS---KVHMALVF-----IPDD--KVYAKVKNFTMS-TGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF-KDGAN---PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1589
S RW T+ EP L IP +LC++ G +D + ++ K L + PR R + +++F K ++ ++ W + + +++ GR L +I + DW EI+ ++P A H + +L R ++ V +G M + +N D L + R + + LVF +P+D + Y +K + + + +QCV ++ R T+ K QM K+G W + + TM VG+D FH + +S+ V S +A+ +++ S K E + L AL + ++MP +I+YRDGVGD QL E + ++ I N + ++VVKKRI+ +FF K G+N P PGTV+D ++ + +Y+F++VSQ GT +PT+YNV+ DT +SP +Q++TY L H+Y+N G IRVP P HYAH+LA LVG+ IH+ N + RLFYL
Sbjct: 379 SQGRWKKGLTGTQREP--------ILLIP-QLCHMTGLTDEICKDYSIVKELAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGRRMVKANSQGDWS-REIR-ELPLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDG-DANSYIDTLRKYTRPTLQMGMSCLLVFKVICILPNDDKRRYDSIKRYLCTKCPIPSQCVVKKTLEKVQAR---TIVTKIAQQMNCKMGGALWKVETDVQRTMFVGIDCFHDIVNRQKSIAGFVASTNAELTKWYSQCVIQKTGEELVKELEICLKAALDVWCKNESSMPHSVIVYRDGVGDGQLQALLDHEAKKMSTYLKTISPNNF--TLAFIVVKKRINTRFFLKHGSNFQNPPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDT----------IGLSPDTVQRLTYCLCHMYYNLPGIIRVPAPCHYAHKLAYLVGQSIHQ-----EPNRSLSTRLFYL 882 HSP 2 Score: 65.0846 bits (157), Expect = 1.648e-60 Identity = 74/280 (26.43%), Postives = 128/280 (45.71%), Query Frame = -1
Query: 1591 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVV----MENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTS-------KWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNE----NGTASTLLYLER----INRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISR 2367
K G +G VV L +N+ + + Y Y+V +E+ ++RT +L + + G+ FDG L + +W S +N+V VE S L T S + + NI+F + E++GR ++ R G S +I GY T++ + N + LL +E I R + ++ R + ++LIG ++TKYNNKTYR+ DI + + D D KI+Y++Y++Q++ + KQP ++S+
Sbjct: 117 KTGSEGTVVQLLANHFRVISRPQWVAYKYNVDYKPDIEDGNLRT----ILLDQHRRKFGERHIFDGNSLLLSRPLKERRVEWLSTTKDKNIVKITVEFSKELTPT----SPDCLRYYNILFRRTFKLLDFEQVGRNYYTKKKAIQL-YRHGTSL------EIWLGYVTSVLQYENSITLCADVSHKLLRIETAYDFIKRTSAQAQTGNI-REEVTNKLIGSIVLTKYNNKTYRVDDIDWKQNPEDTFNKSDGSKITYIDYYRQQHKEIVTVKKQPLLVSQ 380
BLAST of SMEDWI-1 vs. Ensembl Celegans
Match: prg-1 (Piwi-like protein [Source:UniProtKB/TrEMBL;Acc:P90786]) HSP 1 Score: 199.519 bits (506), Expect = 7.860e-59 Identity = 151/542 (27.86%), Postives = 253/542 (46.68%), Query Frame = -2
Query: 54 TENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDF-CKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNN------FIEDVKR--EIGRLRINYTMD-SISSCRSNEVEDALTDFIRV---SKVHMALVFIPDDKV--YAKVKNF-TMSTGLLTQCVTQRN--GSNRDDRRRKTVAD---KSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFN-TMPLRLIIYRDGVGDSQLAFTKKFE-----------TDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA------NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1556
+E +P+ P E Q I ELC+ G +D R + + K + + P++RL + R + K + K WG+ + + ++ R L + + + +W G + + +G+ +++ P N+ FIE+ +R +I ++++ M I N+ + + I+ +HM +V + DD Y +K F + + QCV R G ++D K + K V+QM K G W +N+ + TMIVG D +H T K ++V A V + S F+Q+ S + + NL AL + + + T+P RLI+YRDG GD Q+ + K E TD ++ K+ E L +++V KR++++ K G+ NP PGTVVD + +P +FYLV Q +GT +P +YN++ D + + P QQ+ + L HLY+NW GT+RVP P YAH+LA L + +H N +R++LF+L
Sbjct: 306 SEGKPKQPGEPPQVSYIVPELCFPTGLTDEMRKDFKMMKEIAKHTRMSPQQRLVESRKLIVDLSKNEKVMECFKYWGISLGQDLANVQARVLKSEPLQGKKTYEGKQA--EWARGVKECGIYRGSNMTNWIVI---GPGSGNSGLLSQKFIEEARRLGKILQVQLGEPMCVPIRGISPNDYLEGVKGAIKQVDGEDIHMLVVMLADDNKTRYDSLKKFLCVECPIPNQCVNLRTLAGKSKDGGENKNLGSIVLKIVLQMICKTGGALWKVNIPLKNTMIVGYDLYHDSTLKGKTVGACVSTTSNDFTQFYSQTRPHENPTQLGNNLTHFVRKALKQYYDSNDQTLPSRLILYRDGAGDGQIPYIKNTEVKLVRDACDAVTDKAAELSNKVQEKIKLA---FIIVTKRVNMRILKQGSSLDNAINPQPGTVVDTTVTRPERMDFYLVPQFVNQGTVTPVSYNIIHDD----------TDLGPDKHQQLAFKLCHLYYNWQGTVRVPAPCQYAHKLAFLTAQSLHDDA-----NGCLRDKLFFL 824 HSP 2 Score: 48.521 bits (114), Expect = 7.860e-59 Identity = 80/303 (26.40%), Postives = 133/303 (43.89%), Query Frame = -1
Query: 1585 ALQPDTVTDKIG--KDGRVVSLKSNYANFNIKVEG-----PFYMYDVVMENKSIRTKL--ELLLAYANSKNN-GQPFCFDGRRLFTTSKWHSDEDTE-NLVIKDVEISIRLLTT--FLKNSEEYYQMINIV----FNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRD-------------QIDQLIG-RDIITKYNNKTYRIS--DIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVK 2394
+QPD + G K G + +ANF I +E Y Y V E ++ +K E +L N + G F FDG L+ +W ++ E I I IR T FL + + + N + F+ +Q+ ++GR +F G R P + N IL GY T I R EN +L +E +++ E ++ ++ ++ +++++ G IIT+YNNK +R + D +++ V+ G + I+ YFK +Y I + D QP +IS K
Sbjct: 28 TIQPDLFIRQQGQSKTGSSGQPQKCFANF-IPIEMTQSDYSIYQYHVEFE-PTVDSKANREKMLRDNNVTDEIGHHFVFDGMILYLKEEWEQNQMIEVQHPIDRSLICIRFKQTNRFLVDDPQTINIFNTIIRRSFDALQL----TQLGRNYF-----NWGDSRAVPDY----NMSILPGYETAI-RMYEEN----FMLCVENRFKMVREESMYILFHKELRSCQNNPQRVQEKMNEMYGGTTIITRYNNKLHRYTRLDYSISPLSEFVKDG-QSITLKEYFKNQYGIEITVDDQPIIISEGK 309
BLAST of SMEDWI-1 vs. Ensembl Celegans
Match: alg-2 (pep chromosome:WBcel235:II:874160:879001:1 gene:WBGene00000106.1 transcript:T07D3.7b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-2) HSP 1 Score: 124.02 bits (310), Expect = 1.124e-28 Identity = 127/499 (25.45%), Postives = 212/499 (42.48%), Query Frame = -2
Query: 132 IPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGK--SKDYMKSWGMCIEEQPITIRGREL-APVDIFSNEVKINTKIPDD--WKFGEIKFDVPKGTAHRFGVLVVDRNPSHF-NNFIEDVKREIGRLRINYTMDSISS---CR-SNEVEDALTDFIRV----SKVHMALVFIPDD-KVYAKVKNFTMST-GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKM----------APTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMK---MIEKIYENQTLPQIIYVVVKKRISVKFF-KDGA-------NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLA 1508
+PG+ C L +D + S T++K R R+ CK + + + +G+ I ++GR L AP ++ + T +P+ W +F G R + SH N + ++ R+ + M I + C+ ++ VE F + S + + +V +P +YA+VK + G+ TQCV +N R ++M KLG G+N + P + +G D H G K S+ A V S+ A S+Y + V + + E +L L F P R+++YRDGV + QL ++E A+ + M+E Y+ P I ++ V+KR + F D A N PGT VD I P ++F+L S +GT+ P++Y+VL D N+++ LQQ+TY + H Y ++ +P P +YAH +A
Sbjct: 376 VPGQRC-LKKLTDVQTS---------TMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHT--GMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAI----RTTPQTLSNLCLKMNVKLG----GVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQ----PGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWD-------DNDLTA---DELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVA 840
BLAST of SMEDWI-1 vs. Ensembl Celegans
Match: alg-2 (pep chromosome:WBcel235:II:873176:878945:1 gene:WBGene00000106.1 transcript:T07D3.7a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-2) HSP 1 Score: 123.635 bits (309), Expect = 1.693e-28 Identity = 127/499 (25.45%), Postives = 212/499 (42.48%), Query Frame = -2
Query: 132 IPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGK--SKDYMKSWGMCIEEQPITIRGREL-APVDIFSNEVKINTKIPDD--WKFGEIKFDVPKGTAHRFGVLVVDRNPSHF-NNFIEDVKREIGRLRINYTMDSISS---CR-SNEVEDALTDFIRV----SKVHMALVFIPDD-KVYAKVKNFTMST-GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKM----------APTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMK---MIEKIYENQTLPQIIYVVVKKRISVKFF-KDGA-------NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLA 1508
+PG+ C L +D + S T++K R R+ CK + + + +G+ I ++GR L AP ++ + T +P+ W +F G R + SH N + ++ R+ + M I + C+ ++ VE F + S + + +V +P +YA+VK + G+ TQCV +N R ++M KLG G+N + P + +G D H G K S+ A V S+ A S+Y + V + + E +L L F P R+++YRDGV + QL ++E A+ + M+E Y+ P I ++ V+KR + F D A N PGT VD I P ++F+L S +GT+ P++Y+VL D N+++ LQQ+TY + H Y ++ +P P +YAH +A
Sbjct: 395 VPGQRC-LKKLTDVQTS---------TMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHT--GMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAI----RTTPQTLSNLCLKMNVKLG----GVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQ----PGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWD-------DNDLTA---DELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVA 859
BLAST of SMEDWI-1 vs. Ensembl Celegans
Match: alg-1 (pep chromosome:WBcel235:X:13951262:13956261:1 gene:WBGene00000105.1 transcript:F48F7.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-1) HSP 1 Score: 119.398 bits (298), Expect = 4.016e-27 Identity = 94/320 (29.38%), Postives = 144/320 (45.00%), Query Frame = -2
Query: 132 SKVHMALVFIPDD-KVYAKVKNFTMST-GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKM----------APTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMK---MIEKIYENQTLPQIIYVVVKKRISVKFF----KD----GANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLA 1013
S + + +V +P VYA+VK + G+ TQCV +N R ++M KLG G+N + P + G D H G K S+ A V S+ A S+Y + V + + E +L L F P R+++YRDGV + Q ++E A+ + M+E+ Y+ P I ++ V+KR + F KD N PGT VD I P ++FYL S +GT+ P++Y+VL D N +E LQQ+TY + H Y ++ +P P +YAH +A
Sbjct: 656 SGIQLVVVVLPGKTPVYAEVKRVGDTVLGIATQCVQAKNAI----RTTPQTLSNLCLKMNVKLG----GVNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIISDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQ----PGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDN--NLTADE--------LQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVA 953
BLAST of SMEDWI-1 vs. Ensembl Celegans
Match: alg-1 (pep chromosome:WBcel235:X:13949114:13956261:1 gene:WBGene00000105.1 transcript:F48F7.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-1) HSP 1 Score: 119.398 bits (298), Expect = 4.030e-27 Identity = 94/320 (29.38%), Postives = 144/320 (45.00%), Query Frame = -2
Query: 132 SKVHMALVFIPDD-KVYAKVKNFTMST-GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKM----------APTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMK---MIEKIYENQTLPQIIYVVVKKRISVKFF----KD----GANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLA 1013
S + + +V +P VYA+VK + G+ TQCV +N R ++M KLG G+N + P + G D H G K S+ A V S+ A S+Y + V + + E +L L F P R+++YRDGV + Q ++E A+ + M+E+ Y+ P I ++ V+KR + F KD N PGT VD I P ++FYL S +GT+ P++Y+VL D N +E LQQ+TY + H Y ++ +P P +YAH +A
Sbjct: 677 SGIQLVVVVLPGKTPVYAEVKRVGDTVLGIATQCVQAKNAI----RTTPQTLSNLCLKMNVKLG----GVNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIISDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQ----PGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDN--NLTADE--------LQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVA 974
BLAST of SMEDWI-1 vs. Ensembl Fly
Match: piwi (gene:FBgn0004872 transcript:FBtr0080166) HSP 1 Score: 211.075 bits (536), Expect = 5.183e-70 Identity = 144/477 (30.19%), Postives = 233/477 (48.85%), Query Frame = -2
Query: 120 IPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDF-CKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWK--FGEIK-FDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGR--LRINYTMD-SISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKV--YAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSIS-AKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTL--PQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVG 1508
IP ELC + G + RSN L + + + + P++R + +R F + +S ++ W M +++ ++GR + +I + K+ DW+ F + + P R+ V+ RN ++ + R LRI + I R+ A+ D +R S + L +P+D Y+ +K + + TQ VT + NR ++A K +Q+ KLGY PW I L ++ M +G D S + KR+ A + S+ + S Y S V + L AL +Q++ +P R++ YRDGV L +FE +++ ++ Y L PQ+ Y+VV + ++ +FF +G NP PGT+VD+ I P Y+FYLVSQ+ +GT SPT+YNVL + +SP +Q++TY + HLY+NW GT RVP YA +LA LVG
Sbjct: 364 IP-ELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRAMDDCVR-SDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRS---LMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMG----------LSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVG 825 HSP 2 Score: 75.0998 bits (183), Expect = 5.183e-70 Identity = 75/283 (26.50%), Postives = 130/283 (45.94%), Query Frame = -1
Query: 1591 QPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMEN--KSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIK-DVE--ISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFF-LGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRD----------IITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISR 2388
+P + K G DG V L++N+ K E Y V E ++ R ++ +L +AN +G + FDG +LFTT K+ + + K D+E ISI+ + + Q++N++ + E +GR F + E IRE FKM+ + GY T+I + + LL E ++V+ + + RR + +D ++T YNN+TYRI+D+ + IS++ Y+ +YNI +++ QP +IS+
Sbjct: 82 RPAELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVRMGVLSNHANLLGSG--YLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDPRAKIE---IRE----FKME---LWPGYETSIRQHEKD-----ILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISK 347
BLAST of SMEDWI-1 vs. Ensembl Fly
Match: piwi (gene:FBgn0004872 transcript:FBtr0340227) HSP 1 Score: 211.075 bits (536), Expect = 5.183e-70 Identity = 144/477 (30.19%), Postives = 233/477 (48.85%), Query Frame = -2
Query: 120 IPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDF-CKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWK--FGEIK-FDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGR--LRINYTMD-SISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKV--YAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSIS-AKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTL--PQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVG 1508
IP ELC + G + RSN L + + + + P++R + +R F + +S ++ W M +++ ++GR + +I + K+ DW+ F + + P R+ V+ RN ++ + R LRI + I R+ A+ D +R S + L +P+D Y+ +K + + TQ VT + NR ++A K +Q+ KLGY PW I L ++ M +G D S + KR+ A + S+ + S Y S V + L AL +Q++ +P R++ YRDGV L +FE +++ ++ Y L PQ+ Y+VV + ++ +FF +G NP PGT+VD+ I P Y+FYLVSQ+ +GT SPT+YNVL + +SP +Q++TY + HLY+NW GT RVP YA +LA LVG
Sbjct: 364 IP-ELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRAMDDCVR-SDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRS---LMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMG----------LSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVG 825 HSP 2 Score: 75.0998 bits (183), Expect = 5.183e-70 Identity = 75/283 (26.50%), Postives = 130/283 (45.94%), Query Frame = -1
Query: 1591 QPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMEN--KSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIK-DVE--ISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFF-LGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRD----------IITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISR 2388
+P + K G DG V L++N+ K E Y V E ++ R ++ +L +AN +G + FDG +LFTT K+ + + K D+E ISI+ + + Q++N++ + E +GR F + E IRE FKM+ + GY T+I + + LL E ++V+ + + RR + +D ++T YNN+TYRI+D+ + IS++ Y+ +YNI +++ QP +IS+
Sbjct: 82 RPAELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVRMGVLSNHANLLGSG--YLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDPRAKIE---IRE----FKME---LWPGYETSIRQHEKD-----ILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISK 347
BLAST of SMEDWI-1 vs. Ensembl Fly
Match: aub (gene:FBgn0000146 transcript:FBtr0112793) HSP 1 Score: 196.438 bits (498), Expect = 9.137e-67 Identity = 145/485 (29.90%), Postives = 239/485 (49.28%), Query Frame = -2
Query: 117 DQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGK-SKDYMKSWGMCIEEQPITIRGRELAPVDI-FSNEVKINTKIPDDWKFGEIKFDVPKGTAH--RFGVLVVDRNPSHFNNFIEDVKREIGRLRINYT---MDSISSCRSNEVEDALTDFIRVS-KVHMALVFIPDDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRR--KTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSISAKFS-QYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYEN---QTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK 1523
DQ++ I EL G +D+ R++ + + + P R+ +R F K + K S + +KSW + ++ + I R L P I F N+ DW E + H R+ V+ RN F++ R +++N + I R+ A+ + ++ M ++ P+++ Y+ +K T + + +Q VT + + R + ++A K V+QM +KL PW + + + M VG D HS K +S A V ++ K S +Y S VN E E + N AL ++Q + ++P R++ +RDGVGD QL E + + +++IY++ Q ++ +++V KRI+ ++F NP PGTVVD+ I P Y+F+LVSQ GT SPT+YNV+ D N LQ ++Y +TH+Y+N+ GTIRVP HYAH+LA LV +
Sbjct: 305 DQAIMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSWNIELDSALVEIPARVLPPEKILFGNQKIFVCDARADWT-NEFRTCSMFKNVHINRWYVITPSRNLRETQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSCIKKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGFDVCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPERILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYFTGHRNPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADK----------LQMLSYKMTHMYYNYSGTIRVPAVCHYAHKLAFLVAE 778 HSP 2 Score: 78.9518 bits (193), Expect = 9.137e-67 Identity = 74/296 (25.00%), Postives = 139/296 (46.96%), Query Frame = -1
Query: 1555 QPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTE---NLVIK-----DVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFK--ILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQID-------QLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEKN 2388
+P +T K G G +++++NY + Y Y V T+L Y + G + FDG +F +++ + +D+ LV K ++EI I+ + + E +Q++N++ + + + R ++ + + ++NF+ + GY T+I + N+ S + + ++ R N++S RD D ++G I+T YNNKTYRI D+ + + + D +ISY++Y+K+RYNI +++ KQP V+S RP +KN
Sbjct: 25 RPPGMTSKKGVVGTHITVQANYFKVLKRPNWTIYQYRVDFTPDVEATRLRRSFLYEHKGILGG-YIFDGTNMFCINQFKAVQDSPYVLELVTKSRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYY-----------DPQAKINLENFRMQLWPGYQTSIRQHENDILLCSEICH--KVMRTETLYNILSDAIRDSDDYQSTFKRAVMGMVILTDYNNKTYRIDDVDFQSTPLCKFKTNDGEISYVDYYKKRYNIIIRDLKQPLVMS-------RPTDKN 299
BLAST of SMEDWI-1 vs. Ensembl Fly
Match: aub (gene:FBgn0000146 transcript:FBtr0080165) HSP 1 Score: 196.438 bits (498), Expect = 1.026e-66 Identity = 145/485 (29.90%), Postives = 239/485 (49.28%), Query Frame = -2
Query: 117 DQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGK-SKDYMKSWGMCIEEQPITIRGRELAPVDI-FSNEVKINTKIPDDWKFGEIKFDVPKGTAH--RFGVLVVDRNPSHFNNFIEDVKREIGRLRINYT---MDSISSCRSNEVEDALTDFIRVS-KVHMALVFIPDDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRK--TVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFS-QYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYEN---QTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK 1523
DQ++ I EL G +D+ R++ + + + P R+ +R F K + K S + +KSW + ++ + I R L P I F N+ DW E + H R+ V+ RN F++ R +++N + I R+ A+ + ++ M ++ P+++ Y+ +K T + + +Q VT + + R + ++A K V+QM +KL PW + + + M VG D HS K +S A V ++ K S +Y S VN E E + N AL ++Q + ++P R++ +RDGVGD QL E + + +++IY++ Q ++ +++V KRI+ ++F NP PGTVVD+ I P Y+F+LVSQ GT SPT+YNV+ D N LQ ++Y +TH+Y+N+ GTIRVP HYAH+LA LV +
Sbjct: 376 DQAIMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSWNIELDSALVEIPARVLPPEKILFGNQKIFVCDARADWT-NEFRTCSMFKNVHINRWYVITPSRNLRETQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSCIKKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGFDVCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPERILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYFTGHRNPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADK----------LQMLSYKMTHMYYNYSGTIRVPAVCHYAHKLAFLVAE 849 HSP 2 Score: 78.5666 bits (192), Expect = 1.026e-66 Identity = 74/296 (25.00%), Postives = 139/296 (46.96%), Query Frame = -1
Query: 1555 QPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTE---NLVIK-----DVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFK--ILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQID-------QLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEKN 2388
+P +T K G G +++++NY + Y Y V T+L Y + G + FDG +F +++ + +D+ LV K ++EI I+ + + E +Q++N++ + + + R ++ + + ++NF+ + GY T+I + N+ S + + ++ R N++S RD D ++G I+T YNNKTYRI D+ + + + D +ISY++Y+K+RYNI +++ KQP V+S RP +KN
Sbjct: 96 RPPGMTSKKGVVGTHITVQANYFKVLKRPNWTIYQYRVDFTPDVEATRLRRSFLYEHKGILGG-YIFDGTNMFCINQFKAVQDSPYVLELVTKSRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYY-----------DPQAKINLENFRMQLWPGYQTSIRQHENDILLCSEICH--KVMRTETLYNILSDAIRDSDDYQSTFKRAVMGMVILTDYNNKTYRIDDVDFQSTPLCKFKTNDGEISYVDYYKKRYNIIIRDLKQPLVMS-------RPTDKN 370
BLAST of SMEDWI-1 vs. Ensembl Fly
Match: AGO3 (gene:FBgn0250816 transcript:FBtr0299882) HSP 1 Score: 205.682 bits (522), Expect = 7.354e-55 Identity = 151/516 (29.26%), Postives = 254/516 (49.22%), Query Frame = -2
Query: 54 KKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCK-WTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHF-NNFIEDVKREIGRLRINY---TMDSISSCRSNEVEDALTDFIRV-SKVHMALVFIPDDKVYAKVKNFTMS-TGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1562
KK+ P E Q IP ELCYL G D RS+ L + + T + P +R + F + + +++ + SWG+ + I GR++ I+ +++ ++ ++ + ++ K +++ RN + ++++K+ L +N TM S+ R + AL I + +++ + + D YA +K S + +Q + + N D + ++V K V+QM KLG W + + MI G+D++H + + SV A V SI++ +SQ+ S + E L +F AL ++ + +P +IIYRDG+GD QL +E + E + N+ +I Y+VV+KRI+ + F NP PGTVVD+ I K N Y+F+LVSQ +GT +PT+Y VL D P ++Q+++Y L LY+NW GT+R+P YAH+LA L+G+ I R V + + E+LFYL
Sbjct: 377 KKSRGIP-AERENLQFCLIP-ELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQN--DLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYE----IPQFEMVCGNRI--KISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDD----------CNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVA-----EALSEKLFYL 867 HSP 2 Score: 65.4698 bits (158), Expect = 1.893e-10 Identity = 54/201 (26.87%), Postives = 93/201 (46.27%), Query Frame = -1
Query: 1558 KDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKK--ISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 2121
+DVE+ IR+L + Q+ NI+F+ + + K R+ F S P + ++ GY T + + +L + +R+L + V+ + Y+ L+G ++T+YNN+TY+I+DI D N Q K SY+ Y+KQ +NIN+K+ QP + S +K+S P E+
Sbjct: 202 EDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSR---------PKIIPLAKLEVWPGYVTAV-----DEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDIC-FDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYS-IKKSRGIPAER 386
BLAST of SMEDWI-1 vs. Ensembl Zebrafish
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200]) HSP 1 Score: 215.312 bits (547), Expect = 1.775e-75 Identity = 152/502 (30.28%), Postives = 244/502 (48.61%), Query Frame = -2
Query: 54 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDI-RDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTAHRFG---VLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV------SKVHMALVFIP---DDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK--DG--ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1499
E CYL G +D R++ N+ K+L + + P +R I R G ++ + +WG+ E + +++ GR L I DW E++ +P + + RN + ++ + + G + I + E ED +R + M +V +P DK K + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H + GKRS+ A V S++ S++ S E + L + AL + N++P R+I+YRDGVGD L +E +M+ I+ + ++ P++ VVVKKRIS +FF DG ANP PGTV+D ++ +P +Y+F++VSQ G +PT+YNV+ D S + P +Q++TY L H+Y+NW G +RVP P YAH+LA LVG+ IH+ P +N + + L+YL
Sbjct: 382 EFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWS-KEMR-GLPLISCMSLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIR-----MQRAVMIEYEDRQESLLRALQQNVARETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISK--PQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHK---EPNMN--LDDFLYYL 858 HSP 2 Score: 89.3521 bits (220), Expect = 1.775e-75 Identity = 84/307 (27.36%), Postives = 136/307 (44.30%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVY---RRDQIDQ---------LIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L++N+ + Y Y V ME++ +R+ L + + + G+ FDG LF +K + E +E + VEI++ L +S Q NI+F I + ++IGR ++ + P I G+ TTI + +S +L + ++VL V+ R+ DQ L+G I+TKYNNKTYRI DI + N+ + GD +IS+ NYFK +Y +++ + Q ++S VKR
Sbjct: 77 RGGRRRDFHDSGVHTRQLMEHVKESKTGVSGTAIELRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSAL----LFQHEETLGKAHTFDGAILFLPNKLRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDD---------PFNIPQHRLTIWPGFMTTILQYE-----SSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKELVGLIILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKR 365
BLAST of SMEDWI-1 vs. Ensembl Zebrafish
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200]) HSP 1 Score: 215.312 bits (547), Expect = 1.775e-75 Identity = 152/502 (30.28%), Postives = 244/502 (48.61%), Query Frame = -2
Query: 54 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDI-RDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTAHRFG---VLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV------SKVHMALVFIP---DDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK--DG--ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1499
E CYL G +D R++ N+ K+L + + P +R I R G ++ + +WG+ E + +++ GR L I DW E++ +P + + RN + ++ + + G + I + E ED +R + M +V +P DK K + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H + GKRS+ A V S++ S++ S E + L + AL + N++P R+I+YRDGVGD L +E +M+ I+ + ++ P++ VVVKKRIS +FF DG ANP PGTV+D ++ +P +Y+F++VSQ G +PT+YNV+ D S + P +Q++TY L H+Y+NW G +RVP P YAH+LA LVG+ IH+ P +N + + L+YL
Sbjct: 382 EFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWS-KEMR-GLPLISCMSLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIR-----MQRAVMIEYEDRQESLLRALQQNVARETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISK--PQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHK---EPNMN--LDDFLYYL 858 HSP 2 Score: 89.3521 bits (220), Expect = 1.775e-75 Identity = 84/307 (27.36%), Postives = 136/307 (44.30%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVY---RRDQIDQ---------LIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L++N+ + Y Y V ME++ +R+ L + + + G+ FDG LF +K + E +E + VEI++ L +S Q NI+F I + ++IGR ++ + P I G+ TTI + +S +L + ++VL V+ R+ DQ L+G I+TKYNNKTYRI DI + N+ + GD +IS+ NYFK +Y +++ + Q ++S VKR
Sbjct: 77 RGGRRRDFHDSGVHTRQLMEHVKESKTGVSGTAIELRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSAL----LFQHEETLGKAHTFDGAILFLPNKLRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDD---------PFNIPQHRLTIWPGFMTTILQYE-----SSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKELVGLIILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKR 365
BLAST of SMEDWI-1 vs. Ensembl Zebrafish
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 174.096 bits (440), Expect = 5.048e-44 Identity = 139/538 (25.84%), Postives = 244/538 (45.35%), Query Frame = -2
Query: 54 NNHLLYRESSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA--HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVE-------DALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1613
N + +E + K + P G T + L +P EL ++ G + R + K+L + ++ ++ + ++ + WG+ I ++ + +GR L I + T DW E+ D T + + V R + R G + + R + E LT RV V + DD A K + + + +Q + R S ++ ++VA K ++Q+ KLG + W +N+ + M++G+D H + K RSV V S+++ +++ S V E L AL + + P +++IYRDGV D QL + +E ++K E I YE P++ ++VV+KRIS + G++ P+PGTV+D + ++ +FYL++ +G PT+Y + +T + ++P LQ++T+ + HLY+NW GTIRVP P YAH+LA L G+ +H + PAI ++ E+LF+L
Sbjct: 532 NYGITIKELDQPLLIHRPKERSRPGGKVITGEILLLP-ELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICLHSASFVTSPAVDWSR-ELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQ--PQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETIPNYE----PKLAFIVVQKRISTTLYSYGSDHFGTPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNT----------ANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLH---SEPAI--QLSEKLFFL 1046 HSP 2 Score: 69.3218 bits (168), Expect = 1.835e-11 Identity = 61/280 (21.79%), Postives = 118/280 (42.14%), Query Frame = -1
Query: 1585 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDG------RRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKI-SYLNYFKQRYNINLKNDKQPFVISRVK 2367
KIG G + + SNY K + + Y V + + + G+ FDG +RL ++ T+N ++++I I+L +S+ N+V + +G + +GR + + ++ GYST+I ++ L ++ ++VL ++V+ V ++ + + +G +IT+YNN+TYRI DI+ D L D + ++++Y+++ Y I +K QP +I R K
Sbjct: 289 KIGTKGSPIPIGSNYIPICCKNDA-VFQYHVTFTPNVESLSMRFGMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDN---QEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAV---------VILGKHRLQVWPGYSTSI-----KHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQGSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPK 550
BLAST of SMEDWI-1 vs. Ensembl Zebrafish
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 174.096 bits (440), Expect = 5.048e-44 Identity = 139/538 (25.84%), Postives = 244/538 (45.35%), Query Frame = -2
Query: 54 NNHLLYRESSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA--HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVE-------DALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1613
N + +E + K + P G T + L +P EL ++ G + R + K+L + ++ ++ + ++ + WG+ I ++ + +GR L I + T DW E+ D T + + V R + R G + + R + E LT RV V + DD A K + + + +Q + R S ++ ++VA K ++Q+ KLG + W +N+ + M++G+D H + K RSV V S+++ +++ S V E L AL + + P +++IYRDGV D QL + +E ++K E I YE P++ ++VV+KRIS + G++ P+PGTV+D + ++ +FYL++ +G PT+Y + +T + ++P LQ++T+ + HLY+NW GTIRVP P YAH+LA L G+ +H + PAI ++ E+LF+L
Sbjct: 532 NYGITIKELDQPLLIHRPKERSRPGGKVITGEILLLP-ELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICLHSASFVTSPAVDWSR-ELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQ--PQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETIPNYE----PKLAFIVVQKRISTTLYSYGSDHFGTPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNT----------ANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLH---SEPAI--QLSEKLFFL 1046 HSP 2 Score: 69.3218 bits (168), Expect = 1.835e-11 Identity = 61/280 (21.79%), Postives = 118/280 (42.14%), Query Frame = -1
Query: 1585 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDG------RRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKI-SYLNYFKQRYNINLKNDKQPFVISRVK 2367
KIG G + + SNY K + + Y V + + + G+ FDG +RL ++ T+N ++++I I+L +S+ N+V + +G + +GR + + ++ GYST+I ++ L ++ ++VL ++V+ V ++ + + +G +IT+YNN+TYRI DI+ D L D + ++++Y+++ Y I +K QP +I R K
Sbjct: 289 KIGTKGSPIPIGSNYIPICCKNDA-VFQYHVTFTPNVESLSMRFGMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDN---QEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAV---------VILGKHRLQVWPGYSTSI-----KHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQGSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPK 550
BLAST of SMEDWI-1 vs. Ensembl Zebrafish
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 124.02 bits (310), Expect = 2.228e-28 Identity = 108/462 (23.38%), Postives = 197/462 (42.64%), Query Frame = -2
Query: 279 NNHLLYRESSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA--HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVE-------DALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDT 1613
N + +E + K + P G T + L +P EL ++ G + R + K+L + ++ ++ + ++ + WG+ I ++ + +GR L I + T DW E+ D T + + V R + R G + + R + E LT RV V + DD A K + + + +Q + R S ++ ++VA K ++Q+ KLG + W +N+ + M++G+D H + K RSV V S+++ +++ S V E L AL + + P +++IYRDGV D QL + +E ++K E I YE P++ ++VV+KRIS + G++ P+PGTV+D + ++ +FYL++ +G PT+Y + +T
Sbjct: 532 NYGITIKELDQPLLIHRPKERSRPGGKVITGEILLLP-ELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICLHSASFVTSPAVDWSR-ELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQ--PQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETIPNYE----PKLAFIVVQKRISTTLYSYGSDHFGTPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNT 985 HSP 2 Score: 69.707 bits (169), Expect = 1.347e-11 Identity = 61/280 (21.79%), Postives = 118/280 (42.14%), Query Frame = -1
Query: 1585 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDG------RRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKI-SYLNYFKQRYNINLKNDKQPFVISRVK 2367
KIG G + + SNY K + + Y V + + + G+ FDG +RL ++ T+N ++++I I+L +S+ N+V + +G + +GR + + ++ GYST+I ++ L ++ ++VL ++V+ V ++ + + +G +IT+YNN+TYRI DI+ D L D + ++++Y+++ Y I +K QP +I R K
Sbjct: 289 KIGTKGSPIPIGSNYIPICCKNDA-VFQYHVTFTPNVESLSMRFGMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDN---QEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAV---------VILGKHRLQVWPGYSTSI-----KHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQGSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPK 550
BLAST of SMEDWI-1 vs. Ensembl Xenopus
Match: cd74 (CD74 molecule [Source:Xenbase;Acc:XB-GENE-922847]) HSP 1 Score: 218.394 bits (555), Expect = 1.598e-69 Identity = 159/520 (30.58%), Postives = 255/520 (49.04%), Query Frame = -2
Query: 54 ENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPD--DWKFGEIKFDVPKGTAH--RFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPD---DKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1553
+N P GP L IP E C+L G +D RS+ N+ K+L + P +R + F K S + ++ WG+ + + + GR +AP + K P DW E++ H + +L RN N +++ + G++ I ++ EV+D +++V + V M + + DK A K + + +QCV + + + +VA K +QM K+G + W + + + MIVG+D +H + +GKRS+ A V S++ +++ S K E + L AL + N ++P R+IIYRDGVGD QL ++E + I E P++ +VVKKR+S +FF NP PGT+VD +I +P +Y+F+++SQ G+ SPT+YNV+ D+ + P +Q++TY L HLY+NW G IRVP P YAH+LA LVG+ IHR P + + +RL+YL
Sbjct: 373 QNVPAGPI-----LLIP-EFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGR-VAPAEKILQAGKTADYNPQFADWS-RELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIK-----MNRAVMVEVDDTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNK--PQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSA-EKDYSPKLTVIVVKKRVSARFFAHVGGRLQNPPPGTIVDVEITRPEWYDFFIISQSVRVGSVSPTHYNVVYDS----------GALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHR---EPHLT--LSDRLYYL 861 HSP 2 Score: 67.0106 bits (162), Expect = 1.598e-69 Identity = 68/284 (23.94%), Postives = 124/284 (43.66%), Query Frame = -1
Query: 1579 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVI------KDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSN---CESGGIREGPSFFKM-----DNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGD---KKISYLNYFKQRYNINLKNDKQPFVISRVKRS 2367
K G G ++ L +N+ + + + Y + ME++ +R L + + + G+ FDG LF + D N V + V+I+I L S +Q NI+F + M ++IGR ++ ++ S G+ P F +N + S + R + + L Y+ +N+ + + + +D LIG+ ++TKYNNKTYRI DI D E +IS+++Y++ +YN + + QP ++ K+
Sbjct: 105 KTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCAL----LFQHEELIGKARAFDGTMLFLPKRL----DKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLR------SETVLDYMYNVNQKVESHKFHDICSKD----LIGQIVLTKYNNKTYRIDDI-NWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNPKKP 369
BLAST of SMEDWI-1 vs. Ensembl Xenopus
Match: cd74 (CD74 molecule [Source:Xenbase;Acc:XB-GENE-922847]) HSP 1 Score: 218.394 bits (555), Expect = 1.639e-69 Identity = 159/520 (30.58%), Postives = 255/520 (49.04%), Query Frame = -2
Query: 54 ENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPD--DWKFGEIKFDVPKGTAH--RFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPD---DKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1553
+N P GP L IP E C+L G +D RS+ N+ K+L + P +R + F K S + ++ WG+ + + + GR +AP + K P DW E++ H + +L RN N +++ + G++ I ++ EV+D +++V + V M + + DK A K + + +QCV + + + +VA K +QM K+G + W + + + MIVG+D +H + +GKRS+ A V S++ +++ S K E + L AL + N ++P R+IIYRDGVGD QL ++E + I E P++ +VVKKR+S +FF NP PGT+VD +I +P +Y+F+++SQ G+ SPT+YNV+ D+ + P +Q++TY L HLY+NW G IRVP P YAH+LA LVG+ IHR P + + +RL+YL
Sbjct: 353 QNVPAGPI-----LLIP-EFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGR-VAPAEKILQAGKTADYNPQFADWS-RELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIK-----MNRAVMVEVDDTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNK--PQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSA-EKDYSPKLTVIVVKKRVSARFFAHVGGRLQNPPPGTIVDVEITRPEWYDFFIISQSVRVGSVSPTHYNVVYDS----------GALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHR---EPHLT--LSDRLYYL 841 HSP 2 Score: 67.0106 bits (162), Expect = 1.639e-69 Identity = 68/283 (24.03%), Postives = 124/283 (43.82%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVI------KDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSN---CESGGIREGPSFFKM-----DNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQL---GDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G ++ L +N+ + + + Y + ME++ +R L + + + G+ FDG LF + D N V + V+I+I L S +Q NI+F + M ++IGR ++ ++ S G+ P F +N + S + R + + L Y+ +N+ + + + +D LIG+ ++TKYNNKTYRI DI D E +IS+++Y++ +YN + + QP ++ K+
Sbjct: 85 KTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCAL----LFQHEELIGKARAFDGTMLFLPKRL----DKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLR------SETVLDYMYNVNQKVESHKFHDICSKD----LIGQIVLTKYNNKTYRIDDI-NWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNPKK 348
BLAST of SMEDWI-1 vs. Ensembl Xenopus
Match: piwil3 (piwi-like RNA-mediated gene silencing 3 [Source:NCBI gene;Acc:100496286]) HSP 1 Score: 202.216 bits (513), Expect = 8.974e-69 Identity = 152/517 (29.40%), Postives = 244/517 (47.20%), Query Frame = -2
Query: 54 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDI---------------FSNEVKINTKIP----DDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV---------SKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1499
ELC L G +D R++ + ++L + P +R + F + ++ + ++ WG+ ++Q + GR P I +S E++ T I D+W P+ LV N V ++IG +R+N + R EV+D +I+V V L I +K + K + +QCV R S + +VA K MQM K+G + W + + + M++G+D +H + +GKRS+ V S++ +++ S K E + L +L + N +P R++IYRDGVGD QL +E + I+ + P++ +VVKKRI+ +FF NP PGTVVD ++ +P +Y+F++VSQ +GT SPT+YNV+ D+ S + P +Q++TY L HLY+NW G IRVP P YAH+LA LVG+ IH+ P + + RL+YL
Sbjct: 393 ELCNLTGLTDRMRNDFGVMRDLAVHTRLPPDQREKQVARFLNYIHKDENVQKELRDWGLNFDKQLLPFVGRVSPPERILQAGRTADYNPQFADWSREMRGQTLISVRHLDNWMLL-----YPRRCYDAAQTLVQTLN---------KVAQQIG-MRMN-------NVRMFEVDDRANSYIQVLNAEVNPDTQMVVCVLSSIHKEKYDSIKKYLCLQIPTPSQCVVARTLSK--PQTVLSVATKIAMQMNCKMGGELWSVEIPLGEMMVIGIDCYHDTLSGKRSIAGFVASMNRNLTRWYSRCVLQDQKQEIVDGLKVCLQASLHAWFNCNKNLPSRILIYRDGVGDGQLKTLVNYEIPQFLDCIKSAGRDYC-PKLSIIVVKKRINARFFAHLGGRIQNPPPGTVVDVEVTRPEWYDFFIVSQSVRQGTVSPTHYNVVYDS----------SGLKPDYMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHK---EPHLA--LSNRLYYL 869 HSP 2 Score: 80.4925 bits (197), Expect = 8.974e-69 Identity = 76/287 (26.48%), Postives = 134/287 (46.69%), Query Frame = -1
Query: 1567 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED--TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV----YR----RDQID----QLIGRDIITKYNNKTYRISDI-KEMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRP 2367
K G G + L +N+ + + Y Y + ME++ +RT L Y + + G+ FDG LF + + + ++ ++V+I+I L S +Q NI+F + M ++IGR ++ S+ P N I G++T+I + S +L L+ ++VL V+ V YR RD D +L+G+ ++TKYNNKTYRI +I M + D IS+++Y++ +YN +++ +Q + + +R VRP
Sbjct: 115 KTGVSGSTIQLSTNHIKLVSRPQWVLYQYHIDYNPQMESRRLRTAL----LYQHEELIGKARAFDGTMLFAPKRLNKATEVFSQTRNGENVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPSD---------PIEISQHNLTIWPGFTTSILQYE-----TSIMLSLDVSHKVLRSETVLDVMTKLYRMHGDRDFADAVTKELLGQIVLTKYNNKTYRIDEICWNMTPQSTFKKSDGSDISFVDYYRMQYNKEIRDLRQALIANIPRR--VRP 381
BLAST of SMEDWI-1 vs. Ensembl Xenopus
Match: bckdhb (branched chain keto acid dehydrogenase E1, beta polypeptide [Source:Xenbase;Acc:XB-GENE-953785]) HSP 1 Score: 211.46 bits (537), Expect = 5.273e-67 Identity = 153/527 (29.03%), Postives = 254/527 (48.20%), Query Frame = -2
Query: 54 AQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREP---RERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDV-PKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPDDK--VYAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKR-SVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQT--LPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1571
A K K + P+ P +++ + ELCYL G S S+ L K+L + +P ++RL + D + T ++ ++ WG+ +++Q I + GR P IF + P DW V + +L RN N ++ + + + + M I +VED F+R + + + IP ++ Y +K F ++ + +QCV R + + +VA+K MQM KLG + W + + + M+VG+D K SV V S +A+ +++ S K ++F + L A+ +Q N +P R+++YRDGVGD QL +E + +++ E +T P++ +VV+K+ +F+ NP GT+VD + +P +Y+FYL+SQ +GT +PT YNVL+D + + P +Q++TY L HLY+NW G IRVP P YAH+L LVG+ IHR +P + +LFYL
Sbjct: 328 ALKAKKSDNPDAP----RAVHLIPELCYLTGLSSRALSDFRLMKDLSMETQLDPERRQQRLQRLADSIQRTPEASAE--LEKWGLRMDKQ-IPVTGRIAPPERIFMRDNVCTPGSPADWARDTRNEKVICVAPLQNWLMLFSYRNADVAENLLQCLMKVASGMGFHMDMPRIV-----QVEDNPVSFLRTLQKNVTPDLQLVFCVIPTNQKDSYDSIKKFLSLDQPVPSQCVQNRTLGRQ--QMMMSVANKIAMQMICKLGGELWAVEIPLKALMVVGIDINKDAVNKSMSVVGFVASTNARLTRWHSRCIIQKTTSDFADCLKMCMKGAIQNWQRCNNDLPNRIVVYRDGVGDGQLKMVVDYE---IPQLLSSFRETKTSYCPRLSVIVVRKKCISRFYTQTGRGIQNPVLGTIVDSEATRPEWYDFYLISQNARQGTVNPTYYNVLLDE----------NGLKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLTFLVGQSIHREPSP-----ELSHKLFYL 822 HSP 2 Score: 65.4698 bits (158), Expect = 5.273e-67 Identity = 60/275 (21.82%), Postives = 116/275 (42.18%), Query Frame = -1
Query: 1585 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVV----SVYRRDQID--------QLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVK 2367
K G G V L +N + + Y Y V + KL+ L Y++ + G+ F G LF + + V+I+I L +S Q I+F + + ++GR ++ ++ P + G+ +I + ++ + + + +VL V+ ++Y + + +L+G ++T+YNNKTYR+ DI D D +I+Y +Y+ Q+Y+IN+ + +QP ++S +K
Sbjct: 70 KTGTSGAPVKLMTNLFGLTLPTQWKLYQYRVDFSPEFASRKLKTALLYSHGETLGRARAFHGEILFLSRNIGKVNAFTTRKGETVKITITLTNQLPADSPVCIQFFGILFKKVFKSLNMYQVGRNYYNPAD---------PVIIPQHRLMLWPGFVLSIMKLESQLALCADVSH-----KVLRNETVLDSMKALYSKVPKERFTDICEKELLGLVVLTRYNNKTYRVDDIDWSTKPTDSFPRKDGSEITYTDYYSQQYSINISDLQQPMLVSALK 330
BLAST of SMEDWI-1 vs. Ensembl Xenopus
Match: piwil3 (piwi-like RNA-mediated gene silencing 3 [Source:NCBI gene;Acc:100496286]) HSP 1 Score: 189.119 bits (479), Expect = 7.150e-65 Identity = 151/517 (29.21%), Postives = 239/517 (46.23%), Query Frame = -2
Query: 54 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDI---------------FSNEVKINTKIP----DDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV---------SKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1499
ELC L G +D R++ + ++L + P +R + F + ++ + ++ WG+ ++Q + GR P I +S E++ T I D+W P+ LV N V ++IG +R+N + R EV+D +I+V V L I +K + K + +QCV R S + +VA K MQM K+G + W + + + M++G+D +H + +GKRS+ A FS + F+ L +L + N +P R++IYRDGVGD QL +E + I+ + P++ +VVKKRI+ +FF NP PGTVVD ++ +P +Y+F++VSQ +GT SPT+YNV+ D+ S + P +Q++TY L HLY+NW G IRVP P YAH+LA LVG+ IH+ P + + RL+YL
Sbjct: 403 ELCNLTGLTDRMRNDFGVMRDLAVHTRLPPDQREKQVARFLNYIHKDENVQKELRDWGLNFDKQLLPFVGRVSPPERILQAGRTADYNPQFADWSREMRGQTLISVRHLDNWMLL-----YPRRCYDAAQTLVQTLN---------KVAQQIG-MRMN-------NVRMFEVDDRANSYIQVLNAEVNPDTQMVVCVLSSIHKEKYDSIKKYLCLQIPTPSQCVVARTLSK--PQTVLSVATKIAMQMNCKMGGELWSVEIPLGEMMVIGIDCYHDTLSGKRSI--------AGFSSCVIFI------------LYFLLPASLHAWFNCNKNLPSRILIYRDGVGDGQLKTLVNYEIPQFLDCIKSAGRDYC-PKLSIIVVKKRINARFFAHLGGRIQNPPPGTVVDVEVTRPEWYDFFIVSQSVRQGTVSPTHYNVVYDS----------SGLKPDYMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHK---EPHLA--LSNRLYYL 859 HSP 2 Score: 80.8777 bits (198), Expect = 7.150e-65 Identity = 76/287 (26.48%), Postives = 134/287 (46.69%), Query Frame = -1
Query: 1567 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED--TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV----YR----RDQID----QLIGRDIITKYNNKTYRISDI-KEMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRP 2367
K G G + L +N+ + + Y Y + ME++ +RT L Y + + G+ FDG LF + + + ++ ++V+I+I L S +Q NI+F + M ++IGR ++ S+ P N I G++T+I + S +L L+ ++VL V+ V YR RD D +L+G+ ++TKYNNKTYRI +I M + D IS+++Y++ +YN +++ +Q + + +R VRP
Sbjct: 125 KTGVSGSTIQLSTNHIKLVSRPQWVLYQYHIDYNPQMESRRLRTAL----LYQHEELIGKARAFDGTMLFAPKRLNKATEVFSQTRNGENVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPSD---------PIEISQHNLTIWPGFTTSILQYET-----SIMLSLDVSHKVLRSETVLDVMTKLYRMHGDRDFADAVTKELLGQIVLTKYNNKTYRIDEICWNMTPQSTFKKSDGSDISFVDYYRMQYNKEIRDLRQALIANIPRR--VRP 391
BLAST of SMEDWI-1 vs. Ensembl Mouse
Match: Piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:MGI Symbol;Acc:MGI:1928897]) HSP 1 Score: 201.06 bits (510), Expect = 4.513e-67 Identity = 142/506 (28.06%), Postives = 236/506 (46.64%), Query Frame = -2
Query: 54 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSN--EVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV------SKVHMALVFIPD---DKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1517
++ IP ELCYL G +D R++ N+ K+L + P +R ++ + + + ++ WG+ + ++ GR L I N + D K + + ++ RN N+ I+++ + + I + EV+D ++R S + + + DK A K +QCV R + + +A K +QM K+G + W +++ + MIVG+D +H T G+RS+ V SI+ +++ S E + L AL + MP R+I+YRDGVGD QL +E + ++ + P++ +VVKKR++ +FF NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ D+ S + P +Q++TY L H+Y+NW G IRVP P YAH+LA LVG+ IHR N + RL+YL
Sbjct: 381 AMLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQ-----MKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQ--QTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYN-PRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDS----------SGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHR-----EPNLSLSNRLYYL 862 HSP 2 Score: 75.485 bits (184), Expect = 4.513e-67 Identity = 73/286 (25.52%), Postives = 131/286 (45.80%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSD-----EDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVV----SVYRR-------DQID-QLIGRDIITKYNNKTYRISDIKEMDVNDEV---QLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G +V L +N+ + + Y Y + +ME + +R+ L + + G+ FDG LF + T N + V I+I L S Q NI+F + M ++IGR ++ S+ P I G++T+I + N + +L + ++VL V+ ++Y++ +Q+ +LIG ++TKYNNKTYR+ DI + D N + + ++S+L Y++++YN + + KQP ++S+ KR
Sbjct: 105 KTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSAL----LFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNG--EHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSD---------PIDIPNHRLVIWPGFTTSILQYEN-----NIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKR 369
BLAST of SMEDWI-1 vs. Ensembl Mouse
Match: Piwil4 (piwi-like RNA-mediated gene silencing 4 [Source:MGI Symbol;Acc:MGI:3041167]) HSP 1 Score: 181.8 bits (460), Expect = 3.119e-63 Identity = 135/522 (25.86%), Postives = 241/522 (46.17%), Query Frame = -2
Query: 54 KKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREP---RERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTA-HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVH------MALVFIPDDK--VYAKVKNFTMS-TGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQAS-VFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDG----ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1562
K+ N+ P Q + + ELC+L G S S+ L K + + P +++L + D + + + +++WG+ Q +++ GR + I + DW V +R+ ++ +R F+ ++R G + N I +V++ F+R +VH + + +P ++ Y +K + S + +QCV R + + +VA K MQM KLG + W + + + M+VG+D K V V SI+++ +++ S + + + L AL + + +P R+++YRDGVG+ QL ++E ++K + + + ++ VVV+KR ++ F NP GTVVD + +P +Y+FYL+SQ +GT SPT+YNV+ D + + P +Q++T+ L HLY+NW G I VP P YAH+L LV + +H+ P++ + LFYL
Sbjct: 356 KRKRNDNSEP----QMVHLMPELCFLTGLSSQATSDFRLMKAVAEETRLSPVGRQQQLARLVDDIQRNPVARFE--LETWGLHFGSQ-LSLTGRVVPSEKILLQDHTCQPAFAADWSKDMRSCKVLSSQPLNRWLIVCCNRAEHLIEAFLSCLRRVGGSMGFNVGYPKII-----KVDETPAAFLRAIQVHGDPDVQLVMCILPSNQKNYYDSIKKYLSSDCPVPSQCVLTRTLNKQGTML--SVATKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDALNKNVVVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNRWYRHNHDLPARIVVYRDGVGNGQLKAVLEYEVPQLLKSVTECGSDARSCRLSVVVVRKRCLLRLFASTDHTVQNPPLGTVVDSEATRPEWYDFYLISQTANRGTVSPTHYNVIYDD----------NALKPDHMQRLTFKLCHLYYNWQGLISVPAPCQYAHKLTFLVAQSVHK---EPSLE--LANNLFYL 848 HSP 2 Score: 82.0333 bits (201), Expect = 3.119e-63 Identity = 72/280 (25.71%), Postives = 124/280 (44.29%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWH---SDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERIN-RVLNENNVV------------SVYRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G V L +N N ++ + Y Y V +L + L Y +S + + FDG LF + K ++ +E + ++I++ L + NS Q N++F I + +IGR F+ S P + G++ +++ S LL+ +N +VL V+ S + QL+G ++T+YNNKTYRI DI + Q D +++Y++Y+KQ+Y+I L + QP ++S +KR
Sbjct: 93 KTGSSGIPVRLVTNLFNLDLPQDWQLYQYHVTYSPDLASRRLRIALLYNHSILSDKAKAFDGASLFLSEKLDQKVTELTSETQRGETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQIGRNFYKPSE---------PVEIPQYKLSLWPGFAISVSH------FESKLLFNADVNYKVLRNETVLDFMTDLCLRTGMSCFTEMCHKQLVGLVVLTRYNNKTYRIDDIDWSVKPTQAFQKRDGSEVTYVDYYKQQYDITLSDLNQPVLVSLLKR 357
BLAST of SMEDWI-1 vs. Ensembl Mouse
Match: Piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:MGI Symbol;Acc:MGI:1928897]) HSP 1 Score: 166.392 bits (420), Expect = 1.270e-56 Identity = 120/462 (25.97%), Postives = 210/462 (45.45%), Query Frame = -2
Query: 183 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKS-KDYMKSWGMCIEEQPITIRGRELAPVDIFSN--EVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV------SKVHMALVFIPD---DKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNW 1517
++ IP ELCYL G +D R++ N+ K+L + P +R ++ + + + ++ WG+ + ++ GR L I N + D K + + ++ RN N+ I+++ + + I + EV+D ++R S + + + DK A K +QCV R + + +A K +QM K+G + W +++ + MIVG+D +H T G+RS+ V SI+ +++ S E + L AL + MP R+I+YRDGVGD QL +E + ++ + P++ +VVKKR++ +FF NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ D+ S + P +Q++TY L H+Y+NW
Sbjct: 381 AMLIP-ELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQ-----MKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQ--QTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYN-PRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDS----------SGLKPDHIQRLTYKLCHVYYNW 823 HSP 2 Score: 75.0998 bits (183), Expect = 1.270e-56 Identity = 73/286 (25.52%), Postives = 131/286 (45.80%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSD-----EDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVV----SVYRR-------DQID-QLIGRDIITKYNNKTYRISDIKEMDVNDEV---QLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G +V L +N+ + + Y Y + +ME + +R+ L + + G+ FDG LF + T N + V I+I L S Q NI+F + M ++IGR ++ S+ P I G++T+I + N + +L + ++VL V+ ++Y++ +Q+ +LIG ++TKYNNKTYR+ DI + D N + + ++S+L Y++++YN + + KQP ++S+ KR
Sbjct: 105 KTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSAL----LFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNG--EHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSD---------PIDIPNHRLVIWPGFTTSILQYEN-----NIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDI-DWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKR 369
BLAST of SMEDWI-1 vs. Ensembl Mouse
Match: Piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:MGI Symbol;Acc:MGI:1930036]) HSP 1 Score: 161.384 bits (407), Expect = 5.151e-52 Identity = 116/477 (24.32%), Postives = 220/477 (46.12%), Query Frame = -2
Query: 114 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGK-SKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGT--AHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV----SKVHMALVFI--PDDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKI 1499
EL ++ G + + + K+L + P++ + + + + + + WG+ + + I GR L I T +W E+ D T H + + R + +++ G + + + + + + +E + + K+ M + I D +Y +K + + + +Q + R R ++VA K ++QM KLG + WG+++ + M++G+D +H + G RSV V SI+ +++ S V E ++L + +L + + +P ++++YRDGV D QL +E + K E ++N P+++ VV+K+IS + + P+PGTVVD I + +FYL++ +G PT+Y +++T + +SP +Q++T+ L H+Y+NW GTIRVP P YAH+LA L G+I
Sbjct: 494 ELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNW-VKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQ--PTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEA-FDNYH-PKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNT----------ANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQI 955 HSP 2 Score: 65.0846 bits (157), Expect = 5.151e-52 Identity = 53/192 (27.60%), Postives = 92/192 (47.92%), Query Frame = -1
Query: 1591 DVEISIRL-LTTFLKN-SEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERI-NRVLNENNVVSVYR----------RDQIDQL-IGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISR 2118
D EISI++ LT L+ S+ N+VF + + + +GR F+ ++ + +I GY+ +I R T L L + ++V+ ++V+ V +D+ +L +G +IT+YNN+TYRI D+ D + D K+I++L Y+ + Y I +K D QP +I R
Sbjct: 297 DAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSA---------MVLQQHRLQIWPGYAASIRR------TDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHR 473
BLAST of SMEDWI-1 vs. Ensembl Mouse
Match: Piwil4 (piwi-like RNA-mediated gene silencing 4 [Source:MGI Symbol;Acc:MGI:3041167]) HSP 1 Score: 129.028 bits (323), Expect = 6.093e-30 Identity = 97/387 (25.06%), Postives = 172/387 (44.44%), Query Frame = -2
Query: 54 HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVH------MALVFIPDDK--VYAKVKNFTMS-TGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQAS-VFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1181
+R+ ++ +R F+ ++R G + N I +V++ F+R +VH + + +P ++ Y +K + S + +QCV R + + +VA K MQM KLG + W + + + M+VG+D K V V SI+++ +++ S + + + L AL + + +P R+++YRDGVG+ QL ++E ++K + + G++ Y+FYL+SQ +GT SPT+YNV+ D + + P +Q++T+ L HLY+NW G I VP P YAH+L LV + +H+ P++ + LFYL
Sbjct: 553 NRWLIVCCNRAEHLIEAFLSCLRRVGGSMGFNVGYPKII-----KVDETPAAFLRAIQVHGDPDVQLVMCILPSNQKNYYDSIKKYLSSDCPVPSQCVLTRTLNKQGTML--SVATKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDALNKNVVVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNRWYRHNHDLPARIVVYRDGVGNGQLKAVLEYEVPQLLKSVTEC-------------------------GSDAR--------------YDFYLISQTANRGTVSPTHYNVIYDD----------NALKPDHMQRLTFKLCHLYYNWQGLISVPAPCQYAHKLTFLVAQSVHK---EPSLE--LANNLFYL 878 HSP 2 Score: 77.0258 bits (188), Expect = 8.852e-14 Identity = 74/272 (27.21%), Postives = 123/272 (45.22%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWH---SDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSF----FKMD-NFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
K G G V L +N N ++ + Y Y V +L + L Y +S + + FDG LF + K ++ +E + ++I++ L + NS Q N++F I + +IGR F+ S E P + F D N+K+L NE + L ++ L +S + QL+G ++T+YNNKTYRI DI + Q D +++Y++Y+KQ+Y+I L + QP ++S +KR
Sbjct: 156 KTGSSGIPVRLVTNLFNLDLPQDWQLYQYHVTYSPDLASRRLRIALLYNHSILSDKAKAFDGASLFLSEKLDQKVTELTSETQRGETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQIGRNFYKPSE-----PVEIPQYNKLLFNADVNYKVL----------RNE----TVLDFMTD----LCLRTGMSCFTEMCHKQLVGLVVLTRYNNKTYRIDDIDWSVKPTQAFQKRDGSEVTYVDYYKQQYDITLSDLNQPVLVSLLKR 404
BLAST of SMEDWI-1 vs. UniProt/SwissProt
Match: sp|Q2Q5Y9|PIWI1_SCHMD (Piwi-like protein 1 OS=Schmidtea mediterranea OX=79327 GN=wi-1 PE=2 SV=1) HSP 1 Score: 1049.27 bits (2712), Expect = 0.000e+0 Identity = 503/504 (99.80%), Postives = 504/504 (100.00%), Query Frame = -2
Query: 54 KKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1565
K+KTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL
Sbjct: 305 KEKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 808 HSP 2 Score: 627.091 bits (1616), Expect = 0.000e+0 Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = -1
Query: 1558 MDSTNVTSDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 2478
MDSTNVT+DPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK
Sbjct: 1 MDSTNVTTDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 307
BLAST of SMEDWI-1 vs. UniProt/SwissProt
Match: sp|Q2PC95|PIWIL_DUGJA (Piwi-like protein OS=Dugesia japonica OX=6161 GN=iwi PE=2 SV=1) HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0 Identity = 298/509 (58.55%), Postives = 386/509 (75.83%), Query Frame = -2
Query: 54 KKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKS-WGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK--VHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQ--TLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1565
K+K NE E P + D +L+I GELC+LCGFSD E+SNI+LQKNLG VLKR PRERL+DI FC W + M + WG+ I+ P+ I GREL D+ S +IN KI DDWKFG+++F+V KG H V++VDRN S + NF+ DV+ EI +LR + + + C +N+VE +L D +R +ALVF+PDD+VYAKVK+FT+STGLLTQCVT RNG+NR+D+RR+ V+ K+VMQ+FSK GYDPW I++KM PTMIVG+DTFH+K K+S+ ASVFSI++ FSQY+SF NS KGK EFH+ L NF ALT F+ + +P+R+++YRDGVGDSQL FTK+FE DA+ ++E IY+ +PQIIYV+VKKRI K F G N NPGT+VD++IVKPNF+EF+LVSQ+ T+GTASPTNYNVL DT++ N K + ++P+ LQ+ITY+LTHLYFNWMGTIRVPVP HYAH+LAEL+GK+H+G TP IN+RIR RLFYL
Sbjct: 311 KRKEHNENEAPEDMDLTLNITGELCFLCGFSDQEKSNIDLQKNLGCVLKRGPRERLDDIPSFCDWIKTNNNSQGMSNKWGLKIDNTPLEIEGRELPHCDVISGGQRINEKIKDDWKFGKVQFNVQKGVKHEIDVVIVDRNDSQYGNFLNDVEYEIKQLRFDAKIGRVLVCSANDVERSLNDVMRSGSGCAKIALVFVPDDRVYAKVKSFTISTGLLTQCVTTRNGTNRNDKRRQVVSSKTVMQIFSKFGYDPWTIDIKMRPTMIVGMDTFHNKGSKKSIHASVFSINSTFSQYMSFANSPKGKQEFHDTLSGNFKAALTEFKRIYKILPVRIMVYRDGVGDSQLQFTKQFEVDAMKPLVENIYKENGCQVPQIIYVIVKKRIGTKLFNRGDNANPGTIVDKEIVKPNFFEFFLVSQRVTRGTASPTNYNVLEDTRYQN-KAGIIEAITPNELQRITYTLTHLYFNWMGTIRVPVPVHYAHKLAELIGKVHKGSTPALINERIRNRLFYL 818 HSP 2 Score: 317.005 bits (811), Expect = 0.000e+0 Identity = 153/297 (51.52%), Postives = 211/297 (71.04%), Query Frame = -1
Query: 1558 RRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSI--RTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIRE--GPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 2436
RRGGLQ+RF I+EP LQP+ DKIG G+VV L+SN+ F++ + MYD + + KL++L+ N FC+DGRRLFT+ W D + I + ++S+RL++TFL ++EEYYQMIN++ NN+QI MGQEKIG+ +FLGSN + + GP+FF+ +NFK+LGG+ TT+ RGT NG +TLLY+ERINRVLN+N+V+ Y R IDQLIGRDI+TKYNNKTYRIS+IKEM++N+ ++ + +SY YFK+RYNINL ++QPFV++RVK+ R K K
Sbjct: 17 RRGGLQKRFRIVEPDLQPNETKDKIGNVGKVVRLQSNFTKFSVNKSEKYIMYDTTFSKMGLSPKLKLQILVRICKEYNLPGSFCYDGRRLFTSDNWTEGSDIKEFTIDEKKVSLRLVSTFLSDTEEYYQMINVLLNNLQIIMGQEKIGKGYFLGSNSNKDNVVKNIGPTFFETNNFKVLGGFGTTLQRGTRPNGQLTTLLYIERINRVLNDNSVLVTYNRKNIDQLIGRDILTKYNNKTYRISEIKEMNINETFEMVGRTLSYAQYFKERYNINLTTEQQPFVLTRVKKIARRDKRK 313
BLAST of SMEDWI-1 vs. UniProt/SwissProt
Match: sp|Q2Q5Y8|PIWI2_SCHMD (Piwi-like protein 2 OS=Schmidtea mediterranea OX=79327 GN=wi-2 PE=2 SV=1) HSP 1 Score: 629.017 bits (1621), Expect = 0.000e+0 Identity = 294/496 (59.27%), Postives = 381/496 (76.81%), Query Frame = -2
Query: 54 DQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYM-KSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK--VHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISF-VNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQT--LPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1523
D +L+IPGELC+LCGFSD E+SN++LQKNLG VLKREPRERL+DI +C W + M W + I+ +P+ I GREL P D+ S KIN K+ DDWKFG ++FD+ + H V++VDRN + NF+ DV++E+ +RI+ + +++C N+VE L D R MALVF+PDD+VYAKVK+FTMSTGLLTQCVT RNG+NR+D+RRK V+ K+VMQ+F+K GYDPW + +K+ PTMIVG+DT+H+K+ K+S+QASVFSI++ F+QY+SF VNS KG+ EFHE LGKNF AL F+ +++ +P R++++RDGVGD+QL FTK FE DA+ +IE IY+ +PQIIYV+VKKR+ K F G NPNPGTVVD++IVKPNFYEF+LVSQ+TTKGTA+PTNYNVL DT+ KK + M+P+ LQ+ITY+LTHLYFNWMGTIRVPVP HYAHRLAELVGK+H+G P AIN+RIR RLFYL
Sbjct: 339 DMTLNIPGELCFLCGFSDQEKSNMDLQKNLGCVLKREPRERLDDIPAYCNWIKNSDAATGMCNKWQLKIDNKPLEIEGRELPPCDVISGGSKINEKMGDDWKFGRVQFDIKRDRKHEIDVVIVDRNDFQYKNFMNDVEQELRNMRIDARVGKVNTCGPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRKVVSSKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSINSTFTQYMSFVVNSPKGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKGNGFPVPQIIYVIVKKRVGTKLFNRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKK-GTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHKGSNPVAINERIRNRLFYL 833 HSP 2 Score: 300.442 bits (768), Expect = 0.000e+0 Identity = 144/292 (49.32%), Postives = 201/292 (68.84%), Query Frame = -1
Query: 1570 RGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQP--FCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGI--REGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVR 2433
RGGL+ F I+EP LQP +T+K G GR V L+SN+ F++ F MYD V + K++L + N P FCFDGRRLFT+ KWH + D + + ++++RL++T L +EEYYQMIN++ NN+Q+ +GQE+IG+ +FL N + GP+FF+ ++FK+LGG+ TT+ RGT G +TLLY+ERINRVLN+N+V+ Y R IDQLIGRDIITKYNNKTYRIS+IKEM+V+++ ++G + +SY YFK+RYNI L QPFV++RVK+ M R
Sbjct: 25 RGGLRGTFRIVEPNLQPADITEKTGNIGRTVKLQSNFTKFSVLRSEKFMMYDSVFSQVGLSPKVKLQFIVRVCQENKLPGAFCFDGRRLFTSEKWHGESDIQEFSHDEKKMTLRLVSTILPETEEYYQMINVLLNNLQVMLGQERIGKGYFLSPNITKDEVPRNSGPTFFETNSFKVLGGFGTTLQRGTKPTGELTTLLYIERINRVLNDNSVMKAYNRRSIDQLIGRDIITKYNNKTYRISEIKEMNVDEKFEMGGRTLSYAEYFKERYNIRLTQGDQPFVLTRVKKPMRR 316
BLAST of SMEDWI-1 vs. UniProt/SwissProt
Match: sp|Q8UVX0|PIWL1_DANRE (Piwi-like protein 1 OS=Danio rerio OX=7955 GN=piwil1 PE=1 SV=1) HSP 1 Score: 215.312 bits (547), Expect = 1.096e-74 Identity = 152/502 (30.28%), Postives = 244/502 (48.61%), Query Frame = -2
Query: 54 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDI-RDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTAHRFG---VLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRV------SKVHMALVFIP---DDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK--DG--ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1499
E CYL G +D R++ N+ K+L + + P +R I R G ++ + +WG+ E + +++ GR L I DW E++ +P + + RN + ++ + + G + I + E ED +R + M +V +P DK K + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H + GKRS+ A V S++ S++ S E + L + AL + N++P R+I+YRDGVGD L +E +M+ I+ + ++ P++ VVVKKRIS +FF DG ANP PGTV+D ++ +P +Y+F++VSQ G +PT+YNV+ D S + P +Q++TY L H+Y+NW G +RVP P YAH+LA LVG+ IH+ P +N + + L+YL
Sbjct: 382 EFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWS-KEMR-GLPLISCMSLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIR-----MQRAVMIEYEDRQESLLRALQQNVARETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISK--PQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYE-PKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDN----------SGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHK---EPNMN--LDDFLYYL 858 HSP 2 Score: 89.3521 bits (220), Expect = 1.096e-74 Identity = 84/307 (27.36%), Postives = 136/307 (44.30%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVY---RRDQIDQ---------LIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L++N+ + Y Y V ME++ +R+ L + + + G+ FDG LF +K + E +E + VEI++ L +S Q NI+F I + ++IGR ++ + P I G+ TTI + +S +L + ++VL V+ R+ DQ L+G I+TKYNNKTYRI DI + N+ + GD +IS+ NYFK +Y +++ + Q ++S VKR
Sbjct: 77 RGGRRRDFHDSGVHTRQLMEHVKESKTGVSGTAIELRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSAL----LFQHEETLGKAHTFDGAILFLPNKLRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDD---------PFNIPQHRLTIWPGFMTTILQYE-----SSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKELVGLIILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKR 365
BLAST of SMEDWI-1 vs. UniProt/SwissProt
Match: sp|A8D8P8|SIWI_BOMMO (Piwi-like protein Siwi OS=Bombyx mori OX=7091 GN=Siwi PE=1 SV=1) HSP 1 Score: 213.386 bits (542), Expect = 1.110e-73 Identity = 140/478 (29.29%), Postives = 236/478 (49.37%), Query Frame = -2
Query: 120 ELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCK-WTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIP-----DDW-KFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSN---EVEDALTDFIRVSKVHMALVFIP---DDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF----KDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVG 1499
ELC G SD R+N L ++L K P +R+ + +F + +T + + + +W + + ++ + I+GR+L P +I N K P + W + K + + + V+ +R +FI+ + + G + + R + E + + I + L + D+ A K T+ + TQ V RN S++ ++A K +Q+ KLG PW +++ + M+VG D H +++ ++S A V ++ + +QY S VN+ E ++G N +A+ F+ K T P R+ IYRDGVGD Q+ + E + K + +IY + ++ +++V KRI+ + F + G NP PGTV+D+ + P Y+FYLVSQ +GT +PT+YNV+ DT N P +Q++TY LTHLYFN +RVP YAH+LA L
Sbjct: 421 ELCRQTGLSDEMRANFKLMRSLDVHTKIGPDKRIEKLNNFNRRFTSTPEVVEELATWSLKLSKELVKIKGRQLPPENIIQAN---NVKYPAGDTTEGWTRDMRSKHLLAIAQLNSWVVITPERQRRDTESFIDLIIKTGGGVGFRMRSPDLVVIRHDGPIEYANMCEEVIARKNPALILCVLARNYADRYEAIKKKCTVDRAVPTQVVCARNMSSKSA---MSIATKVAIQINCKLGGSPWTVDIPLPSLMVVGYDVCHDTRSKEKSFGAFVATLDKQMTQYYSIVNAHTSGEELSSHMGFNIASAVKKFREKNGTYPARIFIYRDGVGDGQIPYVHSHEVAEIKKKLAEIYAGVEI-KLAFIIVSKRINTRIFVQRGRSGENPRPGTVIDDVVTLPERYDFYLVSQNVREGTIAPTSYNVIEDTTGLN----------PDRIQRLTYKLTHLYFNCSSQVRVPSVCQYAHKLAFLAA 881 HSP 2 Score: 87.8113 bits (216), Expect = 1.110e-73 Identity = 73/292 (25.00%), Postives = 133/292 (45.55%), Query Frame = -1
Query: 1570 QPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWH-------SDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVR 2388
+P+ VT K G G + L +NY + Y Y V + + T + L +SK G + FDG L+T ++ H SD T+N + + I I+L Y Q+ NI+ + + +GR +F + + P F +I GY TTI + + LL E ++VL + V+ + Y++ ++ ++G+ ++T YN +TYR+ D+ + ++ D+ I+Y+ Y+ ++YN+ +++ QP +ISR K +R
Sbjct: 136 RPEAVTSKKGTSGTPLDLLANYFTVETTPKWGLYQYHVDISPEEDSTGVRKALMRVHSKTLGG-YLFDGTVLYTVNRLHPDPMELYSDRKTDN---ERMRILIKLTCEVSPGDYHYIQIFNIIIRKCFNLLKLQLMGRDYF-----DPEAKIDIPEF----KLQIWPGYKTTINQYEDR-----LLLVTEIAHKVLRMDTVLQMLSEYAATKGNNYKKIFLEDVVGKIVMTDYNKRTYRVDDVAWNVSPKSTFKMRDENITYIEYYYKKYNLRIQDPGQPLLISRSKPREIR 409
BLAST of SMEDWI-1 vs. TrEMBL
Match: D2Z0D8 (DjPiwiA OS=Dugesia japonica OX=6161 GN=DjpiwiA PE=2 SV=1) HSP 1 Score: 963.37 bits (2489), Expect = 0.000e+0 Identity = 452/504 (89.68%), Postives = 483/504 (95.83%), Query Frame = -2
Query: 54 KKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1565
K+K+ENEPEGP E DQSLSIPGELCYLCGFSDSERSN+NLQK+LG VLKREPRERLNDI+DFCKWTG GKSK++M +W M IEEQPITIRGREL PVDI SN+VKIN KIPDDWKFGE+KFDVPKG AHRFGVLVVDRNP+HFNNFI+DVKREIGRLRINYT+D+ISSCR NEVE+AL++F+RV+KVH+ALVFIPDDK+YAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSIS+KFSQYISFVNSSKGKNEFHENLGKNFLTA+TTFQ KF TMP R IIYRDGVGDSQLAFTKKFET+AVMKMIEKIYENQ LPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQ+TTKGTASPTNYNVLMDTK+ NKKTNE+S M+PSVLQQIT+SLTHLYFNWMGTIRVPVPTHYAHRLAELVGK+HRG TPP IN+RIRERLFYL
Sbjct: 306 KEKSENEPEGPKEMDQSLSIPGELCYLCGFSDSERSNLNLQKSLGGVLKREPRERLNDIKDFCKWTGGGKSKNFMTNWNMSIEEQPITIRGRELPPVDIASNDVKINAKIPDDWKFGEVKFDVPKGIAHRFGVLVVDRNPNHFNNFIDDVKREIGRLRINYTIDNISSCRGNEVEEALSEFVRVNKVHLALVFIPDDKIYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISSKFSQYISFVNSSKGKNEFHENLGKNFLTAITTFQQKFGTMPHRFIIYRDGVGDSQLAFTKKFETEAVMKMIEKIYENQNLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQRTTKGTASPTNYNVLMDTKYNNKKTNEISPMAPSVLQQITFSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKVHRGATPPTINERIRERLFYL 809 HSP 2 Score: 534.258 bits (1375), Expect = 7.006e-174 Identity = 258/309 (83.50%), Postives = 281/309 (90.94%), Query Frame = -1
Query: 1555 MDSTNVTSDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVR-PKEKN 2478
MD TNV DP RPRRGGLQRRFAIIEPALQP+T+ DK+GKDGRVV+LKSNYANF+IKVEGPFYMYDVVME KSIR+KLELLLAY +K +GQPFCFDGRRLFTT+KW+ +D E + +VEISIRLLTTFLKNSEEYYQMINIVFNNIQ+FMGQEKIGRQFFLGSNCESGGIREGPSFFKM+NFKILGGYSTTITRGTN++GTASTLLYLERINRVLNEN+V++VYRRDQIDQLIGRDIITKYNNKTYRISDIKEMD NDE+ L KISY NYFKQRYNINLK DKQPFVISR+KRS PKEK+
Sbjct: 1 MDITNVPRDPASRPRRGGLQRRFAIIEPALQPETIIDKVGKDGRVVNLKSNYANFSIKVEGPFYMYDVVMEPKSIRSKLELLLAYGKTKGDGQPFCFDGRRLFTTTKWNRTDDVEKFTVNNVEISIRLLTTFLKNSEEYYQMINIVFNNIQLFMGQEKIGRQFFLGSNCESGGIREGPSFFKMENFKILGGYSTTITRGTNDDGTASTLLYLERINRVLNENSVLAVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDFNDEIDLNGSKISYSNYFKQRYNINLKTDKQPFVISRIKRSAAHNPKEKS 309
BLAST of SMEDWI-1 vs. TrEMBL
Match: T1E104 (PIWI-1 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1) HSP 1 Score: 832.787 bits (2150), Expect = 0.000e+0 Identity = 380/505 (75.25%), Postives = 444/505 (87.92%), Query Frame = -2
Query: 54 QKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1568
+K+ ENEP+ P E DQSLSIPGELC+LCGFSD+ERSNINLQK+LG+VLKREPRERLNDI DFC WT GK+ +MK WG+ IE+QPITI+GREL VD+FSN +INTK DDWKFGE+KFD+P+G HRFG+LV+DRN + FN FI+D+KRE+ RLRIN T++++SSCR+NE+EDALTDF+RV KVHMA+VFI DDKVYA+VKNFT+STGLLTQCVT RN +NRDDRRRK VADKSVMQMF+KLGYDPWG+ LKM PTM+VGLDTFHSKTGKRS+QASVFSIS KFSQY+SFVNSSKGKNE+H+NL +NF +A+ TF++KFNT+P R IIYRDGVGDSQL FTKK+ETD V+K++EK+YEN LPQIIY++VKKRISVKFFKDG NPNPGT+VDE +VKPNFYEFYLVSQ+TTKGTA+PTNYNVLMDT+ TN + + P LQ ITY+LTHLYFNWMGTIRVPVPTHYAHRLAELVG +HRG PP I+DRIRERLFYL
Sbjct: 298 RKENIENEPDAPKEADQSLSIPGELCFLCGFSDNERSNINLQKSLGSVLKREPRERLNDIADFCHWTKEGKADSFMKKWGVEIEDQPITIKGRELNGVDVFSNRQRINTKSSDDWKFGEVKFDIPQGAQHRFGILVIDRNANQFNQFIDDMKREMSRLRINNTIEAVSSCRANEIEDALTDFVRVQKVHMAIVFIGDDKVYARVKNFTISTGLLTQCVTIRNATNRDDRRRKVVADKSVMQMFAKLGYDPWGVELKMVPTMVVGLDTFHSKTGKRSIQASVFSISHKFSQYLSFVNSSKGKNEYHDNLVQNFTSAIKTFKDKFNTLPQRFIIYRDGVGDSQLYFTKKYETDEVLKLMEKLYENMRLPQIIYIIVKKRISVKFFKDGNNPNPGTIVDEMVVKPNFYEFYLVSQRTTKGTATPTNYNVLMDTQLTNNAGVKAPI-PPGQLQHITYALTHLYFNWMGTIRVPVPTHYAHRLAELVGTVHRGTNPPVISDRIRERLFYL 801 HSP 2 Score: 409.453 bits (1051), Expect = 0.000e+0 Identity = 193/293 (65.87%), Postives = 244/293 (83.28%), Query Frame = -1
Query: 1561 RRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVME-NKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKE 2436
+RGGLQ+RF IIEP LQP + +K+GK+G++VSLKSNYANF +K +GPF MYDV M KS+R KLE+L A A K +P+CFDGRRLFT S+WH+ E ++ +K+ E+S+R LT+FL+N+EEYYQM+NI+FNN+Q FMGQE+IGR FFLGSNC+ R GP+FFK DNFKILGG+STT+TRGTN +GTASTLLYLERINRVLNEN+V ++Y ++Q+D LIGRD+ITKYNNKTYRISDIKE+D+ DE++L DKKISY +YFKQRYNI L + KQPFVISR++R ++ KE
Sbjct: 9 KRGGLQKRFYIIEPVLQPAEIVEKVGKEGKIVSLKSNYANFKLKPKGPFCMYDVKMSVGKSLRGKLEILGAVAALKEPNEPYCFDGRRLFTASEWHNSE-IQSFTVKEEEVSLRFLTSFLENTEEYYQMVNIIFNNVQSFMGQERIGRAFFLGSNCDEVTSRYGPTFFKKDNFKILGGFSTTLTRGTNNDGTASTLLYLERINRVLNENSVYNIYNKNQLDLLIGRDVITKYNNKTYRISDIKEIDIKDEIELNDKKISYADYFKQRYNITLNHAKQPFVISRMRRIAIQRKE 300
BLAST of SMEDWI-1 vs. TrEMBL
Match: G9B7B7 (Piwi A (Fragment) OS=Phagocata kawakatsui OX=1051007 GN=piwiA PE=2 SV=1) HSP 1 Score: 723.005 bits (1865), Expect = 0.000e+0 Identity = 324/465 (69.68%), Postives = 396/465 (85.16%), Query Frame = -2
Query: 171 KKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTI 1565
K+ EN+P+ P E D SLSIPGELC+LCGFSDSERSN+NLQK+LG+VLKREPR+RL DI DFC WT G++ ++ WG+ ++ QP+TI GREL +D+FSN+ IN + DDWKFGEIKFD+P G HRF VLVV+RNP+ F FI+D+KREIGRLR+ YT+D++ SC+SNE+ED L+DF+R+ KVHMA+VFI DDK YA+VKNFT++TGLLTQCVT RN +NRDDRRRK VADK+VMQ+F+KLGYDPW I LKM PTM+VG+DTFHSKTG+RS+QA+VFSIS++FSQY+SFVNS KG+NE+HENL +NF A+ TF++KFNT+P R +IYRDGVGDSQL FTKK+ETDAVM+++E IYEN+ LPQI+YV+VKKRI+VKFFKDG NPNPGT+VDEKIVKPNFYEFYLVSQ+TTKGT++PTNYNVLMDT N+ T M+P LQ TY LTHLYFNWMGTI
Sbjct: 71 KENLENQPDTPREADLSLSIPGELCFLCGFSDSERSNVNLQKSLGSVLKREPRDRLRDISDFCSWTNDGRAAPFIHQWGLMLDNQPVTIIGRELKGIDVFSNKKPINGRQMDDWKFGEIKFDIPVGAQHRFAVLVVERNPAQFTQFIDDMKREIGRLRVTYTIDTVQSCKSNEIEDYLSDFVRIQKVHMAVVFIGDDKNYARVKNFTITTGLLTQCVTVRNATNRDDRRRKVVADKTVMQIFAKLGYDPWSIELKMVPTMVVGMDTFHSKTGRRSIQAAVFSISSRFSQYLSFVNSPKGRNEYHENLSQNFKAAIKTFRDKFNTLPQRFVIYRDGVGDSQLYFTKKYETDAVMRIVESIYENRMLPQIVYVIVKKRIAVKFFKDGNNPNPGTIVDEKIVKPNFYEFYLVSQRTTKGTSTPTNYNVLMDTGLPNQTTVVRVPMTPGQLQHFTYVLTHLYFNWMGTI 535 HSP 2 Score: 81.6481 bits (200), Expect = 3.223e-12 Identity = 41/72 (56.94%), Postives = 54/72 (75.00%), Query Frame = -1
Query: 1561 LIGRDIITKYNN-KTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKE 1773
L+GRD++T+ K ISDIKE+DV +E+ L KISY++YFKQRYNI L N KQPFVIS+++R M+ KE
Sbjct: 1 LLGRDVLTQIQIIKHTEISDIKEIDVTEEIDLNGTKISYMDYFKQRYNITLTNGKQPFVISKMRRVMINVKE 72
BLAST of SMEDWI-1 vs. TrEMBL
Match: G9B7C2 (Piwi B (Fragment) OS=Seidlia auriculata OX=1051009 GN=piwiB PE=2 SV=1) HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0 Identity = 306/493 (62.07%), Postives = 391/493 (79.31%), Query Frame = -2
Query: 54 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTG-AGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK--VHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQT--LPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1517
+L+IPGELC+LCGFSD E+ NI+LQKNLG +LKREPR+RL+DI FC W G K +MK WG+ +E +P+ I GREL PVD+ S K+N +IPDDWKFG ++F ++ +++VDRN ++ FI+DVK E+ L +N + S+ C N++E+ LT +R S+ V MA+VF+PDDKVYA+VKNFTMSTGLLTQCVT RNG+NR D+RR+ VA KSVMQ+FSK GYDPW + +K+ PTMIVGLDT+H+KTGK+S+QASV+SI++ F+QY+SFVNS G+ EFHE LG+NF AL F +K+ P R++IYRDGVGDSQ+ FTK+FE DA+ ++EK+Y+++ +PQIIYVVVKKRIS KFFK+G NP PGT+VD+++VKPNFYEF+LVSQ+TTKGTASPTNYNVL DTK KK + M+P+ LQ+ITY+LTHLYFNWMGTIRVP PTHYAHRLAEL+GKIH+G PP INDRIR+RLFYL
Sbjct: 294 TLNIPGELCFLCGFSDQEKGNIDLQKNLGGILKREPRQRLDDIPAFCDWIGKCEKVNSFMKEWGLRVENRPLEIEGRELPPVDVVSGGQKMNERIPDDWKFGRVRFATSSRKSYEIDIVIVDRNDFNYQKFIQDVKTELKNLNVNAKVTSVEKCGPNDIENILTSIMRQSRDAVKMAMVFVPDDKVYARVKNFTMSTGLLTQCVTTRNGTNRMDKRRQVVASKSVMQIFSKFGYDPWSVEIKLRPTMIVGLDTYHNKTGKKSIQASVYSINSTFTQYLSFVNSPHGRQEFHEVLGRNFAAALQQFHSKYEIYPQRIMIYRDGVGDSQMFFTKQFEADALKPIMEKLYQDKGFPIPQIIYVVVKKRISTKFFKNGQNPEPGTIVDKEVVKPNFYEFFLVSQRTTKGTASPTNYNVLEDTKLLTKK-GSMEPMAPNELQKITYTLTHLYFNWMGTIRVPAPTHYAHRLAELIGKIHKGTNPPVINDRIRDRLFYL 785 HSP 2 Score: 296.59 bits (758), Expect = 0.000e+0 Identity = 140/273 (51.28%), Postives = 202/273 (73.99%), Query Frame = -1
Query: 1561 IGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSI--RTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKD-VEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREG--PSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKE 2364
+G G+ V L SN+A F I +YMYDVV+ + + + KL+++ + PFCFDGRRLFT KWH++ D +D +++IRL+T +EEYYQM+N++FNNIQ+FMGQE+IGR +FLG+N + I +G P++F+++NF++LGG+STT+ RGT +GT+STLLY+ERINRVLNEN+VV+ Y + + D L+GRD+ITKYNNKTYRISDIKEM V ++ L +K+SY +YFK+RYNI L++ QPFV++R++R++VR K+
Sbjct: 1 MGNIGKTVKLASNFAKFQICQLETYYMYDVVLSDLGVAPKAKLKIISKVQEMNDLKGPFCFDGRRLFTGEKWHNESDIREFAAEDDKKVTIRLITNVTAGTEEYYQMVNVMFNNIQVFMGQERIGRAYFLGANTDKRDIAKGSGPTYFEVNNFRVLGGFSTTLQRGTRPDGTSSTLLYIERINRVLNENSVVNAYSKGRADTLVGRDVITKYNNKTYRISDIKEMSVGEQFDLSGRKLSYYDYFKERYNIKLQHSNQPFVLTRMRRTIVRKKK 273
BLAST of SMEDWI-1 vs. TrEMBL
Match: D2Z0D9 (DjPiwiB OS=Dugesia japonica OX=6161 GN=DjpiwiB PE=2 SV=1) HSP 1 Score: 642.884 bits (1657), Expect = 0.000e+0 Identity = 296/500 (59.20%), Postives = 388/500 (77.60%), Query Frame = -2
Query: 54 GPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYM-KSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK--VHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQ--TLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1538
P + D +L+IPGELC+LCGFSD E+SN++LQK+LG+VLKREPRERL+DI FC W + + + M W + IE +P+ I GREL P D+ S KIN KIPDDWKFG ++FD+ + H V++VDRN + NF+ DV++E+ +RI+ + +++C N+VE L D R MALVF+PDD+VYAKVK+FTMSTGLLTQCVT RNG+NR+D+RRK V++K+VMQ+F+K GYDPW + +K+ PTMIVG+DT+H+K+ K+S+QASVFSI++ F+QY+SFVN KG+ EFHE LGKNF AL F+ +++ +P R++++RDGVGD+QL FTK FE DA+ +IE IY++ +PQIIYV+VKKR+ K F G NPNPGTVVD++IVKPNFYEF+LVSQ+TTKGTA+PTNYNVL DT+ KK + M+P+ LQ+ITY+LTHLYFNWMGTIRVPVP HYAHRLAELVGK+H+G +P IN+RIR RLFYL
Sbjct: 330 APQDKDMTLNIPGELCFLCGFSDQEKSNMDLQKSLGSVLKREPRERLDDIPAFCNWIKSSDNANGMCNKWQLKIENKPLEIEGRELPPCDVISGNNKINEKIPDDWKFGRVQFDIKRDRKHEIDVVIVDRNDFQYKNFMSDVEQELRNMRIDARVGKVNTCGPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRKVVSNKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSINSTFTQYMSFVNQPKGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKSNGFPVPQIIYVIVKKRVGTKLFNRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKK-GTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHKGNSPAVINERIRNRLFYL 828 HSP 2 Score: 293.123 bits (749), Expect = 0.000e+0 Identity = 143/289 (49.48%), Postives = 193/289 (66.78%), Query Frame = -1
Query: 1558 FAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQP--FCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGI--REGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 2412
F I+EP LQP + +K G GR V L+SN+ F++ F MYD V I K++L + N P FCFDGRRLFT+ KWH + D + D ++++RL+ T L +EEYYQMIN++ NN+QI MGQE+IG+ +FL N + GP+FF+ ++FK+LGG+ TT+ RGT G +TLLY+ERINRVLN+N+V+ Y R IDQLIGRDIITKYNNKTYRIS+IKEM V+++ ++ + +SY YFK RYNI L QPFV++RVK+ M R + K
Sbjct: 24 FRIVEPNLQPAEIIEKTGNIGRTVKLQSNFTKFSVLRSEKFMMYDSVFTQVGIPPKVKLQFIVRVCQENRLPGAFCFDGRRLFTSEKWHGESDIQEFTYDDKKMTLRLVATILPETEEYYQMINVLLNNLQIMMGQERIGKGYFLSPNITKDEVPRNSGPTFFETNSFKVLGGFGTTLQRGTKPTGELTTLLYIERINRVLNDNSVMKAYNRRSIDQLIGRDIITKYNNKTYRISEIKEMSVDEKFEMSGRTLSYAEYFKDRYNIRLSQGDQPFVLTRVKKPMRRERRK 312
BLAST of SMEDWI-1 vs. Ensembl Cavefish
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:103023762]) HSP 1 Score: 218.394 bits (555), Expect = 2.047e-75 Identity = 159/508 (31.30%), Postives = 256/508 (50.39%), Query Frame = -2
Query: 54 LSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFC-KWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTA---HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIP---DDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYIS-FVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK--DG--ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1514
L IP E CYL G +D R++ N+ K+L + + P +R + + F + ++++ + +WG+ E + + + GR L I + DW E++ +P ++ + + RN + ++ + R G + I + E ED +R + M +V +P DK K + +QCV R S + TVA K +QM K+G + W I + + MIVG+D +H + GKRS+ A V S++ S++ S V S+G+ E + L AL + N +P R+I+YRDGVGD L +E +M+ I+ + E+ +P++ VVVKKRI+ +FF DG NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ D S + P +Q++TY L H+Y+NW G +RVP P YAH+LA LVG+ IH+ P IN + + L+YL
Sbjct: 376 LLIP-EFCYLTGLTDKMRNDFNIMKDLSSHTRLTPEQRESRLNRFITNISRNTEAQNELSTWGLSFENKLLGLTGRVLPAERILQGSRTYDYNPWAADWS-KEMR-GLPLISSMPLESWLMFYTRRNSDVAQSLLQTLNRVSGPMGIR-----MQRAVMIEYEDRQESLLRALQQNVGPDTQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTLSR--PQALMTVATKIALQMNCKMGGELWSIEIPLKQLMIVGIDCYHDTAAGKRSIGALVASLNHGMSRWFSKCVLQSRGQ-EIIDGLKGALKGALKAYWKYNNCLPSRIIVYRDGVGDGMLQSVVNYEVPQIMQSIKTMGED-YMPKLTVVVVKKRIASRFFARIDGKLTNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVFDN----------SGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHK---EPNIN--LDDFLYYL 856 HSP 2 Score: 85.5001 bits (210), Expect = 2.047e-75 Identity = 79/307 (25.73%), Postives = 135/307 (43.97%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVY---RRDQIDQ---------LIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L++N+ + + Y Y V ME++ +R+ L + + + G+ FDG LF + H+ E +E + V+I++ L +S Q NI+F I + ++IGR ++ + P I G+ T I + +S +L + ++VL V+ R+ DQ L+G ++TKYNNKTYRI DI + N+ + GD IS+ +Y+K +Y +++ + Q ++S VKR
Sbjct: 75 RGGRRRDFHDAGVHTRQLMEHVKESKTGVSGSKIELRANFMRLLSRPQWALYQYHVDFKPPMESRRLRSAL----LFQHDETLGKARTFDGAILFLPHRLHNTETVLCSETRHGERVQITVTLTNELPPSSPVCLQFYNIIFRRILRMLNMQQIGRHYYNPDD---------PLNIPQHRLTIWPGFVTNILQYE-----SSIMLCTDVSHKVLRSETVLDFMYNLRKQCGDQRFPDACTKELVGLIVLTKYNNKTYRIDDIAWDHTPNNTFKRGDADISFKDYYKTQYGLDITDGNQVLLVSEVKR 363
BLAST of SMEDWI-1 vs. Ensembl Cavefish
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:103024883]) HSP 1 Score: 159.458 bits (402), Expect = 2.535e-52 Identity = 122/524 (23.28%), Postives = 237/524 (45.23%), Query Frame = -2
Query: 54 FAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIK-FDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDAL----TDFIRVSKVHMALVFIPD--DKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1574
+ K ++P G + + EL ++ G + R + K+L + + + I+ + + ++ + ++ WG+ I + + +GR L I + DW ++ + + + V R + + G + I R + E + + V + + I D +Y+ +K + + +Q + R S ++ +++A K ++QM KLG + W +++ + M++G+D H + K RSV V S+++ +++ S V E L AL + ++ P +++IYRDGV D QL +++E ++K E YE P++ ++VV+KRIS + G P PGTV+D + ++ +F+L++ +G PT+Y + +T + ++P LQ++T+ + HLY+NW GTIRVP P YAH+LA L G+ +H + PAI ++ ++L++L
Sbjct: 602 LVHRPKERSKPGGKQIITGEILLLPELSFMTGIPEKMRKDFRAMKDLTVHINVSAEQHTHSIKQLLQNINSNPEALNELQRWGLEISPEILMTQGRTLPMETICLQSASFVSSSSADWSREVVRDASISSIPLYCWAVFYPRRAAEQAEELVTMFGKVAGPIGIKLERPIRVELRDDRTETYVKSIQSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTISQ--PQKLRSIAQKILLQMNCKLGGELWTVSVPLKHLMVIGVDVHHDVSKKKRSVMGFVASLNSLLTRWYSRVTFQMPNEEIINGFRVCLLAALQKYYEMNHSFPEKIVIYRDGVSDGQLKTVEQYEIPQILKCFETFPNYE----PKLTFIVVQKRISTTLYSYGGERFGTPPPGTVLDHTVTNKDWVDFFLMAHSIRQGCGLPTHYISVYNT----------ANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLH---SEPAI--QLADKLYFL 1104 HSP 2 Score: 67.3958 bits (163), Expect = 2.535e-52 Identity = 62/292 (21.23%), Postives = 122/292 (41.78%), Query Frame = -1
Query: 1585 IEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDG------RRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVK 2403
+E A +P K G G +++ +NY K E + Y V + + + G+ FDG +RL ++ T+N ++++I I++ + + N+V + +G + +GR + SN ++ GYST I + L ++ ++VL ++V+++ ++ + + +G +IT+YNN+TYRI DIK D L D + ++++Y+++ Y I +K QP ++ R K
Sbjct: 337 VEAAREP---LKKAGTKGNSITIGANYIPIRCKNEA-VFQYHVTFTPNVEALSMRFGMMKDHRSTTGEVVAFDGSILYLPKRLDEVVHLKAERKTDN---QEIDIKIQMTKVLPPHCDLCIPFYNVVLRRVMKIVGLKLVGRDHYDPSNA---------IVLGKHRLQVWPGYSTCI-----KETDGGLYLGVDVSHKVLRNDSVLNIMGVIYQQSPEHFQDECTKEFVGAIVITRYNNRTYRIDDIKWSKSPKDSFTLADGSETTFIDYYRKNYGITVKEIDQPLLVHRPK 607
BLAST of SMEDWI-1 vs. Ensembl Cavefish
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:103024883]) HSP 1 Score: 158.303 bits (399), Expect = 6.356e-52 Identity = 122/508 (24.02%), Postives = 234/508 (46.06%), Query Frame = -2
Query: 54 TDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIK-FDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDAL----TDFIRVSKVHMALVFIPD--DKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1526
T + L +P EL ++ G + R + K+L + + + I+ + + ++ + ++ WG+ I + + +GR L I + DW ++ + + + V R + + G + I R + E + + V + + I D +Y+ +K + + +Q + R S ++ +++A K ++QM KLG + W +++ + M++G+D H + K RSV V S+++ +++ S V E L AL + ++ P +++IYRDGV D QL +++E ++K E YE P++ ++VV+KRIS + G P PGTV+D + ++ +F+L++ +G PT+Y + +T + ++P LQ++T+ + HLY+NW GTIRVP P YAH+LA L G+ +H + PAI ++ ++L++L
Sbjct: 649 TGEILLLP-ELSFMTGIPEKMRKDFRAMKDLTVHINVSAEQHTHSIKQLLQNINSNPEALNELQRWGLEISPEILMTQGRTLPMETICLQSASFVSSSSADWSREVVRDASISSIPLYCWAVFYPRRAAEQAEELVTMFGKVAGPIGIKLERPIRVELRDDRTETYVKSIQSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTISQ--PQKLRSIAQKILLQMNCKLGGELWTVSVPLKHLMVIGVDVHHDVSKKKRSVMGFVASLNSLLTRWYSRVTFQMPNEEIINGFRVCLLAALQKYYEMNHSFPEKIVIYRDGVSDGQLKTVEQYEIPQILKCFETFPNYE----PKLTFIVVQKRISTTLYSYGGERFGTPPPGTVLDHTVTNKDWVDFFLMAHSIRQGCGLPTHYISVYNT----------ANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLH---SEPAI--QLADKLYFL 1134 HSP 2 Score: 67.3958 bits (163), Expect = 6.356e-52 Identity = 65/294 (22.11%), Postives = 124/294 (42.18%), Query Frame = -1
Query: 1585 IEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDG------RRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYL--ERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVK 2403
+E A +P K G G +++ +NY K E + Y V + + + G+ FDG +RL ++ T+N ++++I I++ + + N+V + +G + +GR + SN ++ GYST I + G LYL + ++VL ++V+++ ++ + + +G +IT+YNN+TYRI DIK D L D + ++++Y+++ Y I +K QP ++ R K
Sbjct: 367 VEAAREP---LKKAGTKGNSITIGANYIPIRCKNEA-VFQYHVTFTPNVEALSMRFGMMKDHRSTTGEVVAFDGSILYLPKRLDEVVHLKAERKTDN---QEIDIKIQMTKVLPPHCDLCIPFYNVVLRRVMKIVGLKLVGRDHYDPSNA---------IVLGKHRLQVWPGYSTCIKE--TDGG-----LYLGVDVSHKVLRNDSVLNIMGVIYQQSPEHFQDECTKEFVGAIVITRYNNRTYRIDDIKWSKSPKDSFTLADGSETTFIDYYRKNYGITVKEIDQPLLVHRPK 637
BLAST of SMEDWI-1 vs. Ensembl Cavefish
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:103024883]) HSP 1 Score: 158.303 bits (399), Expect = 1.724e-47 Identity = 122/508 (24.02%), Postives = 234/508 (46.06%), Query Frame = -2
Query: 54 TDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIK-FDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDAL----TDFIRVSKVHMALVFIPD--DKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGAN----PNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1526
T + L +P EL ++ G + R + K+L + + + I+ + + ++ + ++ WG+ I + + +GR L I + DW ++ + + + V R + + G + I R + E + + V + + I D +Y+ +K + + +Q + R S ++ +++A K ++QM KLG + W +++ + M++G+D H + K RSV V S+++ +++ S V E L AL + ++ P +++IYRDGV D QL +++E ++K E YE P++ ++VV+KRIS + G P PGTV+D + ++ +F+L++ +G PT+Y + +T + ++P LQ++T+ + HLY+NW GTIRVP P YAH+LA L G+ +H + PAI ++ ++L++L
Sbjct: 618 TGEILLLP-ELSFMTGIPEKMRKDFRAMKDLTVHINVSAEQHTHSIKQLLQNINSNPEALNELQRWGLEISPEILMTQGRTLPMETICLQSASFVSSSSADWSREVVRDASISSIPLYCWAVFYPRRAAEQAEELVTMFGKVAGPIGIKLERPIRVELRDDRTETYVKSIQSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTISQ--PQKLRSIAQKILLQMNCKLGGELWTVSVPLKHLMVIGVDVHHDVSKKKRSVMGFVASLNSLLTRWYSRVTFQMPNEEIINGFRVCLLAALQKYYEMNHSFPEKIVIYRDGVSDGQLKTVEQYEIPQILKCFETFPNYE----PKLTFIVVQKRISTTLYSYGGERFGTPPPGTVLDHTVTNKDWVDFFLMAHSIRQGCGLPTHYISVYNT----------ANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLH---SEPAI--QLADKLYFL 1103 HSP 2 Score: 52.373 bits (124), Expect = 1.724e-47 Identity = 43/170 (25.29%), Postives = 81/170 (47.65%), Query Frame = -1
Query: 1585 NIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYL--ERINRVLNENNVVSV-----------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVK 2049
++ F ++I +G + +GR + SN ++ GYST I + G LYL + ++VL ++V+++ ++ + + +G +IT+YNN+TYRI DIK D L D + ++++Y+++ Y I +K QP ++ R K
Sbjct: 454 HVTFTVMKI-VGLKLVGRDHYDPSNA---------IVLGKHRLQVWPGYSTCIKE--TDGG-----LYLGVDVSHKVLRNDSVLNIMGVIYQQSPEHFQDECTKEFVGAIVITRYNNRTYRIDDIKWSKSPKDSFTLADGSETTFIDYYRKNYGITVKEIDQPLLVHRPK 606
BLAST of SMEDWI-1 vs. Ensembl Cavefish
Match: ago2 (argonaute RISC catalytic component 2 [Source:NCBI gene;Acc:103030212]) HSP 1 Score: 113.235 bits (282), Expect = 3.803e-25 Identity = 97/336 (28.87%), Postives = 145/336 (43.15%), Query Frame = -2
Query: 111 VHMALVFIPDD-KVYAKVKNFTMST-GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMA-----------------PTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLM-DTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLA-----ELVGKIH 1007
+ + +V +P VYA+VK + G+ TQCV +N K+ Q S L IN+K+ P + +G D H G K S+ A V S+ A S+Y + V + + + ++L L F P R+I YRDG+ + Q + E A+ + K+ E P I +VVV+KR + F K G P GT VD KI P+ ++FYL S +GT+ P++Y+VL D FT+ + LQ +TY L H Y ++ +P P +YAH +A LV K H
Sbjct: 528 LQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKN------------VQKTTPQTLSNLCLK---INVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLAAMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFNQVLQHELLAIREACIKL-EKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPA-GTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDE-----------LQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEH 835
BLAST of SMEDWI-1 vs. Ensembl Sea Lamprey
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 191.045 bits (484), Expect = 5.191e-61 Identity = 146/508 (28.74%), Postives = 253/508 (49.80%), Query Frame = -2
Query: 54 QSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAG-KSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPD-DWKFGEIKFDVPKGTAHR-FGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIR-----VSKVHMALVFIP---DDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIE--KIYENQTLPQIIYVVVKKRISVKFFKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1520
Q++ + EL Y+ G +D RS+ ++ K + ++ P R N + +F +++ + +WG+ ++ + GR+L P +I + K P+ DW + A + + VL R+ + F+ + + + + + +I N+ D IR V M + P DD+ Y+ VK + + +Q V R S + +R ++VA K V+QM KLG + W +++ + M+VG+D FH+ GK+SV V S++ S++ S V E + L +A+ + + +P ++I+YRDGV D+QLA + FE VM++ + ++ P+++ VVV+KRIS F + + N G+V+D +++F+LVSQ +GT SPT+Y V+ F + K +S +Q++TY +THLY+NW GT+RVP P YAH+LA LVG+ P + D +LF+L
Sbjct: 290 QTICLIPELAYMTGITDLMRSDFSMMKAISEHMRLGPSLRHNSLMEFISNVKRNPEAQQELSNWGLQMDSDIYKLSGRQLPP-EIIRLQYKAFAFSPEADWTRHIVNTPCITAVAMKNWQVLCTHRDSFLVHEFVTMMCNVGPPMGLVHQLPNIIEL-PNDHTKTYIDCIRKNYSYSQGVQMVMCIFPTLRDDR-YSTVKQVCCVELAVPSQVVQTRTISQQ--KRLRSVAQKVVLQMNCKLGGELWILDIPLKNLMVVGVDVFHNVVLGKQSVVGFVASMNRSVSRWYSRVVRQSTSQELVDGLNLCMTSAIKKYYELNHYLPEKIIMYRDGVSDNQLAIVRDFE---VMQLTNGFRTFDLSYQPKMLVVVVQKRISTNIFLNNSGAMENAPSGSVLDTVATSQEWFDFFLVSQHVRQGTVSPTHYVVI----FNSLK------LSADHVQRLTYKMTHLYYNWSGTVRVPAPCQYAHKLASLVGENLHSDPDPGLCD----KLFFL 775 HSP 2 Score: 62.7734 bits (151), Expect = 5.191e-61 Identity = 69/285 (24.21%), Postives = 116/285 (40.70%), Query Frame = -1
Query: 1582 KIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENK--SIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIK------DVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-------------YRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQL--GDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2367
KIG G + + +NY E Y Y V S T+ LL + + N + FDG L+ K +D NL K ++ +SI+ +S+ + NI+F I + + IG+ + R+ P I GY + + +L L+ +RVL ++V+ V ++ + L+G +IT+YNN+TYRI DI L K +S++ Y+K +Y I + + QP ++ R K+
Sbjct: 15 KIGTYGTPLEIATNYIRLRCSNEA-IYQYHVTFSPDVDSTNTRFGLLNEHKSVIGNTK--AFDGCVLYLPRKL---DDNLNLTSKRISDGVEITLSIKCTKIIKPDSDLCLPIYNIIFKKIMRLLELKLIGKSYL--------NPRQ-PIVINRYRIHIWPGYVVAV-----QKKDGGLMLMLDVSHRVLRMDSVLEVMTHLYESCQSSANFQEECTKALVGHVVITRYNNRTYRIDDIAWDKTPKSTFLNSAGKTMSFVEYYKTQYEITIDDLGQPLLLHRAKQ 279
BLAST of SMEDWI-1 vs. Ensembl Sea Lamprey
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200]) HSP 1 Score: 155.992 bits (393), Expect = 2.581e-50 Identity = 145/509 (28.49%), Postives = 235/509 (46.17%), Query Frame = -2
Query: 54 LSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFC-KWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIP--DDKVYAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFH-SKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENL-----GKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDG----ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1514
L + E+ YL G SD RS+ +L K+LG + P +R + F K ++ + WG+ + Q +++ GR + ++ + + DW + + +L+ N S+ I D ++G + I+ EV D F++ + M + +P Y +K F + +QCV R S R +VA K +QM KLG + W + + + M+VG+D++H S T +SV V S++ +++ S E + L KNF+ +L + + M +IIY L+F Y + P++ VVVKKR +FF+ G NP+PGTV+D + + +Y+FYLVSQ +GT +PT+YNV+ DT S M P +Q++ Y + HLY+NW G IRVP P HYAH+LA LVG+ IH+ P+++ + +RL+YL
Sbjct: 399 LHLLPEISYLTGLSDKMRSDFHLMKDLGLQTQVGPDKRAMTTQKFIEKLQSNPETCNEFAKWGLSFDRQLLSLTGRVVPCEKLYQKDQSYQYNQRQADWSRESRNMKLMTPISLEEWMLLFTHNNSNEARAIVDNLMQVGPPMGMH----INKPEMFEVLDRAQSFVQTLQQNLNHHTQMVMCVLPTIHKGKYDSIKQFLCVERPFPSQCVLARTLSKPQMIR--SVATKIALQMNCKLGGELWSVEIPIKQLMVVGVDSYHDSITRGQSVCGFVASLNQTLTRWYSRCIFQHAAQEMIDGLKVCMQCKNFI-SLQIASHSNDVMINVVIIYF-------LSFLNS-------------YCCGSRPKVTVVVVKKRGIARFFEFGRSGLQNPSPGTVIDTEATRHEWYDFYLVSQSVRQGTVTPTHYNVVYDT----------SNMKPDHMQRLAYKMCHLYYNWPGVIRVPAPCHYAHKLAFLVGQSIHK---EPSLD--LSDRLYYL 865 HSP 2 Score: 62.003 bits (149), Expect = 2.581e-50 Identity = 60/274 (21.90%), Postives = 123/274 (44.89%), Query Frame = -1
Query: 1582 GRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWH--SDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRR--DQIDQ----------LIGRDIITKYNNKTYRISDIKEMDVNDEVQL---GDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2352
G+ V L +NY + Y Y V + +L + L + +++ G+ FDG L+ K S+ ++ + ++I+I L +S + NI+F + + ++IGR ++ + + + G+ T+I ++ +L + +++L +V+ +QI++ L+G+ I+T+YNN+TYRI DI + N + +++Y Y+ + Y++ +K+ +QP +ISR KR
Sbjct: 129 GQPVKLLTNYFKLMSRKMWMVYQYHVTFSPELESKRLRIALLHEHNELLGETRAFDGCILYLPKKLEKVSEVWSKTRQEQKIKITITLTNELPPSSPTAIVLFNIIFRKVLKMVNMQQIGRNYYNPDDAFT---------IPQHRLHVWPGFVTSIMCYESD-----VMLCADVSHKILRNESVLDFMYTLFEQIEERHFHDACQKELVGQVILTRYNNRTYRIDDI-SWNRNPSMTFLKSDGTEVTYYEYYTKHYSLEVKDMQQPLLISRSKR 387
BLAST of SMEDWI-1 vs. Ensembl Sea Lamprey
Match: ago2 (argonaute RISC catalytic component 2 [Source:ZFIN;Acc:ZDB-GENE-110606-6]) HSP 1 Score: 105.145 bits (261), Expect = 3.162e-25 Identity = 78/244 (31.97%), Postives = 110/244 (45.08%), Query Frame = -2
Query: 111 PTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFET----DAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIH 794
P + +G + H G K S+ A V S+ A S+Y + V + E ++LG L F P R+I YRDGV + Q E DA +K+ EK Y+ P I YV V+KR + F K G P T VD KI P+ ++FYL S +GT+ P++Y+VL D N+ S LQ +TY L H Y ++ +P P +YA RL + H
Sbjct: 9 PVIFLGAEVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQHHRQEIIQDLGTMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLFHELLAIRDACIKL-EKDYQ----PGITYVAVQKRHHTRLFCADRNERIGKSGNIPAGTTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWD---DNR-------FSADELQVLTYQLCHTYVRCTRSVSIPAPAYYA-RLVAFRARYH 236
BLAST of SMEDWI-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009610.1 (pep scaffold:Pmarinus_7.0:GL477777:44890:66775:1 gene:ENSPMAG00000008651.1 transcript:ENSPMAT00000009610.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.9214 bits (141), Expect = 4.232e-9 Identity = 53/211 (25.12%), Postives = 89/211 (42.18%), Query Frame = -2
Query: 420 VHMALVFIPDD-KVYAKVKNFTMST-GLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGIN----------LKMAPTMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF 1007
+ + +V +P VYA+VK + G+ TQCV +N + +++ + K+ G+N + P + +G D H G K S+ A V S+ A S+Y + V + + E +L L F P R+I YRDGV + Q E A+ + ++ E + P I ++VV+KR + F
Sbjct: 516 LQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVLK--------TSPQTLSNLCLKINVKLGGVNNILVPHIRPAVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQEIIHDLSSMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLYHELLAIREACIRL-EKEYRPGITFIVVQKRHHTRLF 717
BLAST of SMEDWI-1 vs. Ensembl Nematostella
Match: EDO49931 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFC2]) HSP 1 Score: 202.601 bits (514), Expect = 2.794e-67 Identity = 148/514 (28.79%), Postives = 255/514 (49.61%), Query Frame = -2
Query: 54 DQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDF-CKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPD-DWKFGEIKFDVPKGTAHRFGVLVV--DRNPSHFNNFIEDVKREIGRLRINYT---MDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKV--YAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSIS---AKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA---------NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1523
D L +P ELC+L G SD R++ ++ K+L + +P+ R D+ + + + WG+ + GR L IF N DW EI+ T + ++V +R+ +F + + R G + IN T M ++ R++ AL++ I+ + + +P ++ Y +K + G+ +Q V R S + + +V K +Q+ KLG + W +++ + MIVG+DT+H + K RSV V S++ + +Y S E + L AL +Q +P ++I++RDGVGD QL + E ++ ++ +++ P+ + VVKKRI + F A NP PGTVVD+++ + +++F++VSQ +GT SPT+YNV+ DT + + P QQ++Y LTHLY+NW GT+RVP P YAH+LA LVG+ +H+ P++ ++ ++L++L
Sbjct: 378 DPVLLLP-ELCFLTGLSDDMRADFSVMKDLAVHTRLDPKARSKDLNKLISEINNNPDASGELSGWGLRFGRNLLDFDGRILPGEKIFQKNKGSNYDPKSADWS-REIRGAPLHSTVNLENWVIVHTNRDSGVATDFQQTLARVCGPMGINTTKPRMVPLNDDRTDSYLKALSNEIQADFPQIVVTIVPSNRKDRYDSIKKLCCLEKGVPSQVVVSRTLSKK--QMLMSVCTKIGIQLNCKLGGEAWAVDIPLKNLMIVGIDTYHDSSQKGRSVGGFVASLNKPCTRQREYYSRCTFQHSGQELVDGLKVCMTAALRQWQQINGVLPDKIIVFRDGVGDGQLRAVIEHEVPQILSTFKQ-FQDGYDPKFGFTVVKKRIQTRLFMREARDPDMRNLMNPPPGTVVDKEVTRSEWFDFFIVSQSVRQGTVSPTHYNVIHDT----------TGLRPDHWQQLSYKLTHLYYNWPGTVRVPAPCQYAHKLAFLVGQSLHK---EPSL--KLADKLYFL 871 HSP 2 Score: 72.7886 bits (177), Expect = 2.794e-67 Identity = 68/294 (23.13%), Postives = 124/294 (42.18%), Query Frame = -1
Query: 1582 QPDTVTDKIGKDGRV---VSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIK--------DVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSV-------------YRRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2388
+P T D K+G V V + SNY + + Y Y+V + K L + G FDG LF K D+ T+ + ++ VEI+I L +S + NI+ I ++ +++GR ++ N + ++ GY+T+I + + S LL + ++VL + V+ + + +L+G ++T+YNNKTYR+ DI N +IS+ +Y+K+ Y+ + + +QP + S+ K+
Sbjct: 94 EPTTRRDGFNKEGSVGTKVGIVSNYFRLETRPQAAIYQYNVSFSPEVDFKKARFALIGEQRELLGNIRAFDGMVLFLPKKL-PDQVTKIMAVRTRRETPDEPVEITITLTNELPADSPTCLHLFNIILKRILKYIDMKQVGRNYYYIKNAIA---------IPKHGLELWPGYTTSILKYEH-----SILLGADVSHKVLRKQTVLDFLYELYNKMQGRGNFHDEAAKKLVGEIVLTRYNNKTYRVDDIDWNKRPNSTFTTPKGEISFNDYYKKNYDFTITDQEQPLLFSKPKK 372
BLAST of SMEDWI-1 vs. Ensembl Nematostella
Match: EDO34027 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQI3]) HSP 1 Score: 201.06 bits (510), Expect = 1.668e-62 Identity = 142/515 (27.57%), Postives = 243/515 (47.18%), Query Frame = -2
Query: 54 KKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFC-KWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGT-AHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVS---KVHMALVFIP---DDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDG---ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1562
KKTE + G E L IP ELC L G +D+ R + + K++ + P +R ++ F ++ + + +W + ++ + GR+ P I + DW + V + + V R+ N+F++ + + + I ++ R + E L IR + +V + +V P DD+ A K + + + +Q + + SN R +V K +Q+ KLG + W +++ M+ G+D +H K G RSV V S++ +++ S V E + L + + + + N +P R+I+YRDGVGD QL +E + E+ P++ +VV+KRI+ + F NP PG+V+D + + ++ +F+LVSQ +GT +PT+Y V+ DT S P ++Q+++Y LTHLY+NW GT+RVP P YAH+LA VG+ G + D RLF+L
Sbjct: 254 KKTEQKRGGVIEV--ILLIP-ELCKLTGLNDAMRKDFRVMKDIAAHTRVNPMQRQEAMKKFVMSINSTPEALEQLTAWNLRLDTSTLMTEGRQFPPEKIIYGNTTGSAGDQADWGRDATREKVISAVNLNNWMVFFTQRDSGKANDFVQTMNKCAPAMGIQVNQPKMNQLRDDRTETYLRS-IRENLHPQVQVVVVIFPTSRDDRYAAVKKLCCVESPVPSQVIISKTISNPTKLR--SVVQKIALQINVKLGGELWALDIPSRSLMVCGIDVYHDKARGGRSVGGLVCSMNRSLTRWYSDVCFQSPGQELIDGLKMLLIKGIRKWHDVNNALPERIIVYRDGVGDGQLKVVAGYEVQQFEECFASFGESYK-PKLAVLVVQKRINTRIFSAEPRLGNPGPGSVLDHTVTRKDWNDFFLVSQHVRQGTVTPTHYVVVYDT----------SNWKPDIMQKLSYKLTHLYYNWPGTVRVPAPCQYAHKLAYQVGQSIHGQPSQELCD----RLFFL 747 HSP 2 Score: 58.5362 bits (140), Expect = 1.668e-62 Identity = 46/185 (24.86%), Postives = 89/185 (48.11%), Query Frame = -1
Query: 1579 LTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINR-------VLNENNVVSVYRRDQID-QLIGRDIITKYNNKTYRISDIKEMDVNDEVQ-----LGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRS 2094
T + + +Q+ NI+F + + +++GR F+ N ++ P+ N + GY T++ E G R+ R +LN ++ V RD++ +LIG ++T YNNKTYR+ DI D N ++++++ Y+K+ Y I +++ QP +++R K++
Sbjct: 86 FTRVVPPDDAIFQVYNILFRRVMKILDMQQVGRYFY---NPKT------PAHIPQHNLVLWPGYITSLQH--YEGGLMLLCDVHHRLLRTDSCLDYMLNMHSKVGDRFRDEVSKELIGSVVLTTYNNKTYRVDDI---DWNKRPTSTFTFHNGEQMTFVEYYKKSYEIEIRDLDQPMIVNRPKKT 256
BLAST of SMEDWI-1 vs. Ensembl Nematostella
Match: EDO46431 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQ46]) HSP 1 Score: 116.701 bits (291), Expect = 1.086e-26 Identity = 100/327 (30.58%), Postives = 147/327 (44.95%), Query Frame = -2
Query: 132 FIRVSKVH----MALVFIPDD-KVYAKVKNF--TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAP----------TMIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF---------KDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLA 1025
F R+ + H M LV +P VYA+VK TM G++TQC+ G N +T+++ +++ +KLG G+N +AP + +G D H G + SV A V S+ A S+Y + V + E L L F P R++ YRDGV + Q E A+ + K+ E P I ++VV+KR + F K G P PGT VD I P ++FYL S +GT+ P++Y+VL D N +E LQ +TY L H Y + +P P +YAH +A
Sbjct: 499 FRRLKEAHPDLQMILVVLPGKTPVYAEVKRVGDTM-LGVITQCI---QGKNVTKPSPQTLSN-LCLKINAKLG----GVNNILAPEIRPPVFREPVIFLGADVTHPAAGDDKRPSVAAVVGSMDAHPSRYYASVRVQTHRQEIIAELAAMVRELLVQFYRSTRHKPQRIVFYRDGVSEGQFRQVLVHELKAIREACIKL-EVGYQPGISFIVVQKRHHTRLFCQRDQDKCGKSGNVP-PGTTVDRGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDD--NNFSADE--------LQALTYQLCHTYVRCTRAVSIPAPAYYAHLVA 804
BLAST of SMEDWI-1 vs. Ensembl Nematostella
Match: EDO28124 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7C9]) HSP 1 Score: 48.9062 bits (115), Expect = 1.512e-6 Identity = 42/175 (24.00%), Postives = 70/175 (40.00%), Query Frame = -2
Query: 420 MIVGLDTFHSKT-GKRSVQASVFSISAKFSQYISFVNSS---------------KGKNEFHENLGK----NFLT--------------------------------ALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF 788
M+ G+D +H K G RSV V S++ +++ S V KG ++H+ + K +LT A+ + + N +P R+I+YRDGVGD QL +E + E+ P++ +VV+KRI+ + F
Sbjct: 4 MVCGIDVYHDKARGGRSVGGLVCSMNRSLTRWYSDVCFQSPGQELIDGLKMLLIKGIRKWHDAIRKWHDVCYLTMFVSLSRPSGSGMMPSGSGMMYVIFNVCVLIKAIRKWHDVNNALPERIIVYRDGVGDGQLKVVAGYEVQQFEECFASFGESYK-PKLAVLVVQKRINTRIF 177
BLAST of SMEDWI-1 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 220.705 bits (561), Expect = 2.803e-76 Identity = 159/508 (31.30%), Postives = 256/508 (50.39%), Query Frame = -2
Query: 54 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCK-WTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTA---HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPDDKV--YAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK----DGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1517
++ IP ELCYL G +D RS+ + ++L + P +R++ + F + G ++ +K WG+ + + + R LAP IF + + DW E++ +P ++ + + + RN + + ++ + + G L + + E ED +R K M +V +P ++ Y VK + + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H GKRS+ A V S++ S++ S E + L F AL + N +P R+I+YRDGVGD QL FE +++ I+ + ++ P++ VVVKKRIS +FF +NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ DT S + P +Q++TY L H+Y+NW G IRVP P YAH+LA LVG+ IHR N ++ LFYL
Sbjct: 374 AMLIP-ELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDICGNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWS-REMR-GIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLR-----LQRAVMIEYEDHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSR--PQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAFTGALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYN-PKVSVVVVKKRISSRFFAMINDKVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDT----------SGLKPDHMQRLTYKLCHMYYNWQGLIRVPAPCQYAHKLAFLVGQSIHR-----EPNIKLDNYLFYL 855 HSP 2 Score: 86.2705 bits (212), Expect = 2.803e-76 Identity = 83/307 (27.04%), Postives = 132/307 (43.00%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVS--VYRRDQI----------DQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L +N+ + + Y Y V ME++ +RT L Y +++ G FDG L + H E +E + V I++ L S Q NI+F + + ++IGR ++ N P N I GYSTTI + +S +L + ++VL V+ V R + +LIG ++TKYNNKTYRI DI + + GD IS++NY+K +Y +++ + Q ++S VK+
Sbjct: 73 RGGHRRDFNDFGVNTRQLMEHVKESKCGTSGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTAL----LYRHAEVIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRVLRLLEMQQIGRNYYNPKN---------PLNIPEHNLTIWPGYSTTILQYE-----SSIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQRFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKK 361
BLAST of SMEDWI-1 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 218.779 bits (556), Expect = 1.255e-74 Identity = 162/511 (31.70%), Postives = 257/511 (50.29%), Query Frame = -2
Query: 54 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLND----IRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTA---HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPDDKV--YAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK----DGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1517
++ IP ELCYL G +D RS+ + ++L + P +R++ I D C G ++ +K WG+ + + + R LAP IF + + DW E++ +P ++ + + + RN + + ++ + + G L + + E ED +R K M +V +P ++ Y VK + + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H GKRS+ A V S++ S++ S E + L F TAL + N +P R+I+YRDGVGD QL FE +++ I+ + ++ P++ VVVKKRIS +FF +NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ DT S + P +Q++TY L H+Y+NW G IRVP P YAH+LA LVG+ IHR N ++ LFYL
Sbjct: 341 AMLIP-ELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDIC---GNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWS-REMR-GIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLR-----LQRAVMIEYEDHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSR--PQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAF-TALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYN-PKVSVVVVKKRISSRFFAMINDKVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDT----------SGLKPDHMQRLTYKLCHMYYNWQGLIRVPAPCQYAHKLAFLVGQSIHR-----EPNIKLDNYLFYL 821 HSP 2 Score: 82.8037 bits (203), Expect = 1.255e-74 Identity = 83/307 (27.04%), Postives = 132/307 (43.00%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVS--VYRRDQI----------DQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L +N+ + + Y Y V ME++ +RT L Y +++ G FDG L + H E +E + V I++ L S Q NI+F + + ++IGR ++ N P N I GYSTTI + +S +L + ++VL V+ V R + +LIG ++TKYNNKTYRI DI + + GD IS++NY+K +Y +++ + Q ++S VK+
Sbjct: 43 RGGHRRDFNDFGVNTRQLMEHVKESKCGTSGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTAL----LYRHAEVIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRL---LEMQQIGRNYYNPKN---------PLNIPEHNLTIWPGYSTTILQYE-----SSIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQRFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKK 328
BLAST of SMEDWI-1 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 182.956 bits (463), Expect = 2.034e-64 Identity = 140/473 (29.60%), Postives = 233/473 (49.26%), Query Frame = -2
Query: 165 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLND----IRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTA---HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPDDKV--YAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK----DGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRV 1517
++ IP ELCYL G +D RS+ + ++L + P +R++ I D C G ++ +K WG+ + + + R LAP IF + + DW E++ +P ++ + + + RN + + ++ + + G L + + E ED +R K M +V +P ++ Y VK + + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H GKRS+ A V S++ S++ S E + L F AL + N +P R+I+YRDGVGD QL FE +++ I+ + ++ P++ VVVKKRIS +FF +NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ DT S + P +Q++TY L H+Y+NW +I +
Sbjct: 374 AMLIP-ELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDIC---GNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWS-REMR-GIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLRLQRAVMI-----EYEDHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSR--PQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAFTGALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYN-PKVSVVVVKKRISSRFFAMINDKVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDT----------SGLKPDHMQRLTYKLCHMYYNWQVSISL 822 HSP 2 Score: 84.7297 bits (208), Expect = 2.034e-64 Identity = 83/307 (27.04%), Postives = 132/307 (43.00%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVS--VYRRDQID----------QLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L +N+ + + Y Y V ME++ +RT L Y +++ G FDG L + H E +E + V I++ L S Q NI+F + + ++IGR ++ N P N I GYSTTI + + S +L + ++VL V+ V R + +LIG ++TKYNNKTYRI DI + + GD IS++NY+K +Y +++ + Q ++S VK+
Sbjct: 73 RGGHRRDFNDFGVNTRQLMEHVKESKCGTSGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTAL----LYRHAEVIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRVLRLLEMQQIGRNYYNPKN---------PLNIPEHNLTIWPGYSTTILQYES-----SIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQRFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKK 361
BLAST of SMEDWI-1 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 182.956 bits (463), Expect = 2.034e-64 Identity = 140/473 (29.60%), Postives = 233/473 (49.26%), Query Frame = -2
Query: 165 SLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLND----IRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKIN-TKIPDDWKFGEIKFDVPKGTA---HRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK------VHMALVFIPDDKV--YAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHS-KTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFK----DGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRV 1517
++ IP ELCYL G +D RS+ + ++L + P +R++ I D C G ++ +K WG+ + + + R LAP IF + + DW E++ +P ++ + + + RN + + ++ + + G L + + E ED +R K M +V +P ++ Y VK + + +QCV R S + TVA K +QM K+G + W + + + MIVG+D +H GKRS+ A V S++ S++ S E + L F AL + N +P R+I+YRDGVGD QL FE +++ I+ + ++ P++ VVVKKRIS +FF +NP PGTV+D ++ +P +Y+F++VSQ G+ SPT+YNV+ DT S + P +Q++TY L H+Y+NW +I +
Sbjct: 374 AMLIP-ELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDIC---GNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWS-REMR-GIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLRLQRAVMI-----EYEDHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSR--PQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAFTGALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYN-PKVSVVVVKKRISSRFFAMINDKVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDT----------SGLKPDHMQRLTYKLCHMYYNWQVSISL 822 HSP 2 Score: 84.7297 bits (208), Expect = 2.034e-64 Identity = 83/307 (27.04%), Postives = 132/307 (43.00%), Query Frame = -1
Query: 1582 RGGLQRRF---AIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDV----VMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDED---TENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVS--VYRRDQID----------QLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKR 2433
RGG +R F + L K G G + L +N+ + + Y Y V ME++ +RT L Y +++ G FDG L + H E +E + V I++ L S Q NI+F + + ++IGR ++ N P N I GYSTTI + + S +L + ++VL V+ V R + +LIG ++TKYNNKTYRI DI + + GD IS++NY+K +Y +++ + Q ++S VK+
Sbjct: 73 RGGHRRDFNDFGVNTRQLMEHVKESKCGTSGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTAL----LYRHAEVIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRVLRLLEMQQIGRNYYNPKN---------PLNIPEHNLTIWPGYSTTILQYES-----SIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQRFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKK 361
BLAST of SMEDWI-1 vs. Ensembl Medaka
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:100301588]) HSP 1 Score: 156.762 bits (395), Expect = 1.654e-51 Identity = 131/534 (24.53%), Postives = 239/534 (44.76%), Query Frame = -2
Query: 54 FAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGK-SKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWK--------FGEIKFDV-----PKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIP----DDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKI--YENQTLPQIIYVVVKKRISVKFFKDGANP----NPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGK-IHRGVTPPAINDRIRERLFYL 1574
+ K + P G + L +P EL +L G + +++ K+L + + + I+ K + + ++ WG+ + + I GR L I T W I ++ P+ +A + L+ S F+ + + R D + + LT S+ H+ LV D +Y+ +K + + +Q + R S + ++ + +A K ++Q+ SKLG + W + + + M+VG+D H + K +SV V S+++ + + S V E FL+ L + + +P ++++YRDGV D QL + +E ++K E YE P+++++VV+KRI+ + AN GTV+D + ++ +FYL + +G PT+Y L +T + ++P LQ++T+ + HLY+NW GTIRVP P YAH+LA L G+ +H + PAI ++ ++LF+L
Sbjct: 533 LVHRPKERSNPGGKVISGHILLVP-ELSFLTGIPEKMKNDYRAMKDLKIHINVSSDQHTHAIKQLLKNISSKEDCTKALEGWGLELGSDVMMITGRMLPFETICLKSSSFATGANVSWSREVVNGASISSIPLNIWVVFYPRRSAEQAVELI-----STFHKVAGPIGLRLQRPITVELRDDRTETYVKSIHSQLT-----SEPHLQLVVCVIEGNRDDLYSAIKKLCCLKCPVPSQVINVRTISQQ--QKLRGIAQKILLQLNSKLGGELWTVGIPLKSLMVVGVDVHHDTSKKHQSVMGFVASVNSSLTHWYSRVTFQTPTEELISGFRACFLSTLQKYYELNHDLPQKIVVYRDGVSDGQLKMVELYEIPQLIKCFETFPNYE----PKLVFIVVQKRINTILYSSSANGFSSVPAGTVLDHTLTNKDWVDFYLTAHDIRQGVGLPTHYVTLYNT----------ANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQFLH---SEPAI--QLSDKLFFL 1034 HSP 2 Score: 67.3958 bits (163), Expect = 1.654e-51 Identity = 65/289 (22.49%), Postives = 124/289 (42.91%), Query Frame = -1
Query: 1585 EPALQPDT-VTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKD---VEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVV----SVYRRDQ-------IDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGD-KKISYLNYFKQRYNINLKNDKQPFVISRVK 2400
EP ++ ++ +K G G +++ SN+ + K + Y Y V + + + G+ FDG L+ + + +++ D +EI+I++ NS+ N+VF + +G +GR + N ++ GYST I R L ++ ++VL ++V+ ++Y++ + +LIG IT+YNN+TYRI DI+ + D L D + ++L Y+ + Y I ++ QP ++ R K
Sbjct: 265 EPTVETESKPLNKAGVKGASITIGSNHIPISCKNQA-VYQYHVTFNPNVESMSMRFGMMRDHRSTTGEVIAFDGSILYLPVQLCDVVNLKSVRRTDDEEIEITIKMTKVLPPNSDLCIPFYNVVFRRVMKVIGLRLVGRNHYDPENA---------VILGKQRLQVWPGYSTAIKRMD-----GGLYLAVDVSHKVLRNDSVLDLMNTLYQQTKENFKDACTKELIGSIAITRYNNRTYRIDDIEWDKSPKDSFTLMDGTETTFLEYYSKNYGITVQETNQPLLVHRPK 538
BLAST of SMEDWI-1 vs. Planmine SMEST
Match: SMESG000036375.1 (SMESG000036375.1) HSP 1 Score: 1049.65 bits (2713), Expect = 0.000e+0 Identity = 503/504 (99.80%), Postives = 504/504 (100.00%), Query Frame = -2
Query: 54 KKKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1565
K+KTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL
Sbjct: 305 KEKTENEPEGPTETDQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 808 HSP 2 Score: 627.861 bits (1618), Expect = 0.000e+0 Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = -1
Query: 1558 MDSTNVTSDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 2478
MDSTNVTSDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK
Sbjct: 1 MDSTNVTSDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQPFCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEK 307
BLAST of SMEDWI-1 vs. Planmine SMEST
Match: SMESG000069984.1 (SMESG000069984.1) HSP 1 Score: 634.024 bits (1634), Expect = 0.000e+0 Identity = 294/495 (59.39%), Postives = 381/495 (76.97%), Query Frame = -2
Query: 54 DQSLSIPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFCKWTGAGKSKDYM-KSWGMCIEEQPITIRGRELAPVDIFSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSK--VHMALVFIPDDKVYAKVKNFTMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGKRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQT--LPQIIYVVVKKRISVKFFKDGANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1523
D +L+IPGELC+LCGFSD E+SN++LQKNLG VLKREPRERL+DI +C W + M W + I+ +P+ I GREL P D+ S KIN K+ DDWKFG ++FD+ + H V++VDRN + NF+ DV++E+ +RI+ + +++C N+VE L D R MALVF+PDD+VYAKVK+FTMSTGLLTQCVT RNG+NR+D+RRK V+ K+VMQ+F+K GYDPW + +K+ PTMIVG+DT+H+K+ K+S+QASVFSI++ F+QY+SFVNS KG+ EFHE LGKNF AL F+ +++ +P R++++RDGVGD+QL FTK FE DA+ +IE IY+ +PQIIYV+VKKR+ K F G NPNPGTVVD++IVKPNFYEF+LVSQ+TTKGTA+PTNYNVL DT+ KK + M+P+ LQ+ITY+LTHLYFNWMGTIRVPVP HYAHRLAELVGK+H+G P AIN+RIR RLFYL
Sbjct: 343 DMTLNIPGELCFLCGFSDQEKSNMDLQKNLGCVLKREPRERLDDIPAYCNWIKNSDAATGMCNKWQLKIDNKPLEIEGRELPPCDVISGGSKINEKMGDDWKFGRVQFDIKRDRKHEIDVVIVDRNDFQYKNFMNDVEQELRNMRIDARVGKVNTCGPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRKVVSSKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSINSTFTQYMSFVNSPKGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKGNGFPVPQIIYVIVKKRVGTKLFNRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKK-GTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHKGSNPVAINERIRNRLFYL 836 HSP 2 Score: 303.138 bits (775), Expect = 0.000e+0 Identity = 145/292 (49.66%), Postives = 202/292 (69.18%), Query Frame = -1
Query: 1570 RGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSNYANFNIKVEGPFYMYDVVMENKSIRTKLELLLAYANSKNNGQP--FCFDGRRLFTTSKWHSDEDTENLVIKDVEISIRLLTTFLKNSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGI--REGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVYRRDQIDQLIGRDIITKYNNKTYRISDIKEMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVR 2433
RGGL+R F I+EP LQP +T+K G GR V L+SN+ F++ F MYD V + K++L + N P FCFDGRRLFT+ KWH + D + + ++++RL++T L +EEYYQMIN++ NN+Q+ +GQE+IG+ +FL N + GP+FF+ ++FK+LGG+ TT+ RGT G +TLLY+ERINRVLN+N+V+ Y R IDQLIGRDIITKYNNKTYRIS+IKEM+V+++ ++G + +SY YFK+RYNI L QPFV++RVK+ M R
Sbjct: 25 RGGLRRTFRIVEPNLQPADITEKTGNIGRTVKLQSNFTKFSVLRSEKFMMYDSVFSQVGLSPKVKLQFIVRVCQENKLPGAFCFDGRRLFTSEKWHGESDIQEFSHDEKKMTLRLVSTILPETEEYYQMINVLLNNLQVMLGQERIGKGYFLSPNITKDEVPRNSGPTFFETNSFKVLGGFGTTLQRGTKPTGELTTLLYIERINRVLNDNSVMKAYNRRSIDQLIGRDIITKYNNKTYRISEIKEMNVDEKFEMGGRTLSYAEYFKERYNIRLTQGDQPFVLTRVKKPMRR 316
BLAST of SMEDWI-1 vs. Planmine SMEST
Match: SMESG000081970.1 (SMESG000081970.1) HSP 1 Score: 194.512 bits (493), Expect = 5.461e-60 Identity = 145/516 (28.10%), Postives = 247/516 (47.87%), Query Frame = -2
Query: 54 PEGPTETDQSLS--IPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFC-KWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDI-FSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNP-SHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIR-----VSKVHMALVFIPDDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA-------NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 1544
P+G E D L IP ELC L G +D + L + L + P++R + +F ++K + WG+ IE+ + I ++ P I F NE ++ ++K V G +L+ ++ + N ++ + + R+ + + I ++ E+ L + R V V L + +D+ Y +K F + + +Q V + + ++ + +VA +Q+ +KLG + W + + + M+VGLD +H G+ RSV A V S +A +++ S + + E L L+ L + + N +P + +YRDGV D QL + +K E + K+ E + P ++Y VV+KRI+ + + NPNPGT+VD + + N Y+F+LVSQ +GT +PT+Y VL D S +P +Q + Y H+Y+NW GT+RVP P YAH+LA +VG+ + + D RLFYL
Sbjct: 478 PKGSKEVDGGLVYLIP-ELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDR-YNAIKKFCYVDCPVPSQVVLAQ--TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFES-FSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDN----------SKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCD----RLFYL 974 HSP 2 Score: 57.7658 bits (138), Expect = 5.461e-60 Identity = 46/176 (26.14%), Postives = 82/176 (46.59%), Query Frame = -1
Query: 1591 NSEEYYQMINIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVY-------------RRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISR 2076
N E + N++F I+ + KIGR +F E +I GY T+I +N LL + ++V+ ++V + + I+QL+G+ ++T +NN+ YRI+DI + E G +KISY +YFK Y + +++ +QP ++S+
Sbjct: 306 NFSECPMVYNVLFKRIENSLRMVKIGRDYFYP---------EKKIVLDRRRMEIWPGYVTSI-----QNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSK 467
BLAST of SMEDWI-1 vs. Planmine SMEST
Match: SMESG000050994.1 (SMESG000050994.1) HSP 1 Score: 117.087 bits (292), Expect = 2.458e-26 Identity = 102/353 (28.90%), Postives = 162/353 (45.89%), Query Frame = -2
Query: 102 VEDALTDFIRVS-----KVHMALVFIPDDKVYAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPT------------MIVGLDTFHSKTGK----RSVQASVFSISAKFSQYISFVNSSKGKNEFH--------ENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFF----KDG----ANPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGV 1046
+E TD IR S K+ + + + +D+ Y +VK F + G++TQC+ + R+ D+ + + K+ G+N +++P M+ G+D H + + +SV A V S+ + Y + + K E E++ N L +T ++ P RLI YRDGV + Q K E A+ +K+ E P I Y+VV+KR ++FF KDG N GTVVD+ I ++FYL S + +GT+ P +Y+VL D N ++E LQ TY L H+Y ++ P PT+YAH LA G+ G+
Sbjct: 888 IEKVATDTIRNSEKEKKKLDLLVFVVINDEDYKEVKKFGDVLFGIVTQCMKYATLN------RQKFLDQYLGNIMLKVNGKLGGVNWELSPIRNRLFTPIKDSFMVFGIDVTHPASTRNDLSKSVAAMVGSLDNNATMYSALIRQQLSKKERAIIEIVMEMEDMVYNLL--MTFYKANKQLKPKRLIFYRDGVSEGQFDIVFKHEIIAIQSACQKL-EKDYQPPITYIVVQKRHKIRFFPQNEKDGVGRSGNVPSGTVVDKDITHCREFDFYLCSHEGIQGTSKPAHYHVLFDE--NNWGSDE--------LQSFTYFLCHIYARCTRSVSYPAPTYYAH-LAAFRGRTLIGL 1220
BLAST of SMEDWI-1 vs. Planmine SMEST
Match: SMESG000051000.1 (SMESG000051000.1) HSP 1 Score: 110.538 bits (275), Expect = 2.270e-24 Identity = 116/470 (24.68%), Postives = 205/470 (43.62%), Query Frame = -2
Query: 114 ERLNDIRDFCKWTGAGKSKDYMKSWGMCIEEQPITIRGREL-APVDIFSNEVKINTKIPD------DWKFGEIKFDVPKGTAHRFGVLVVDRNPSHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIRVSKVHMALVFIPDDKVYAKVKNF-TMSTGLLTQCVTQRNGSNRDDRRRKTVAD---KSVMQMFSKLGYDPWGI-NLKMAPT----MIVGLDTFHSKTG---KRSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFN------------TMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKF----FKDGA----NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMDTKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKI 1406
ER ++++ ++ Y++S+ + I Q I ++GR L P +F ++ K+P + KF + D K FG N + ++ + ++ + D+ SN+ + I+ + + +V + +D Y VK+ + G++TQC+ N R+K ++ ++++ +KLG W I N++ AP M+ G+D H +SV A V SI+ + Y + + K+ GK+ + + ++ + +P LI YRDGV + Q E A+ EK +N P+I Y++V+KR +++F +D N PG V+D I ++FYL S + +GT+ P +Y V++D N LQ+ TY L ++ F +I P PTHYAH LA G+I
Sbjct: 469 ERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDK---EEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNTFCYVSNDDIKKSIEKIK-EDLDLLVVVVRNDVEYKLVKDIGDIELGIVTQCICYENLC-----RQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKD------GKDAVETVVEMEDMVHRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYK-PKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSND----------LQEFTYFLCYVCFRCTRSISYPAPTHYAH-LAAFRGRI 911 The following BLAST results are available for this feature:
BLAST of SMEDWI-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SMEDWI-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMEDWI-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of SMEDWI-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMEDWI-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of SMEDWI-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMEDWI-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007406 ID=SMED30007406|Name=SMEDWI-1|organism=Schmidtea mediterranea sexual|type=transcript|length=2730bpback to top protein sequence of SMED30007406-orf-1 >SMED30007406-orf-1 ID=SMED30007406-orf-1|Name=SMED30007406-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=309bp MDSTNVTSDPNLRPRRGGLQRRFAIIEPALQPDTVTDKIGKDGRVVSLKSback to top protein sequence of SMED30007406-orf-2 >SMED30007406-orf-2 ID=SMED30007406-orf-2|Name=SMED30007406-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=523bp MTNNHLLYRESSDRWFAQKKKTENEPEGPTETDQSLSIPGELCYLCGFSDback to top Aliases
The feature 'SMEDWI-1' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
