SMED30007360
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007360 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30007360 aligns in the following genomic locations:
Homology
BLAST of SMED30007360 vs. TrEMBL
Match: A0A1Z1R2Q3 (Estrella OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 142.895 bits (359), Expect = 3.667e-35 Identity = 85/283 (30.04%), Postives = 146/283 (51.59%), Query Frame = 1 Query: 151 IQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKINNLKYLMTTDTNITTK---ILPPTGVSSFQINWRRPGKS--SEHIELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKRLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIMLSDFEIEPIL---SCKNGYDSNCYIFLNSAKRFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIPKHFIQ 975 +QVN+SN+ Y +L + +A PL ILDV L+ C++N T ++ +PIIFW+ SY+ + N M+ N ++ ILPP+G S+++NWRR + I +D+S+ + ++Y LP F++EF LC E KN L+ E Q E V+ F I + I + D + + C G S C + + A++++Y +++ ++K +S +++LQ IY + L LC KV S+ +R+ + ++ L C IP++ Q Sbjct: 30 VQVNISNIIYKQLMTTKDNVLAASPLAILDVNLLKKHKCYKNIRTNSWSFMMPIIFWDDSYMTNVANSIQKMSKLKNFVSEKPFILPPSGFRSYRLNWRRDLNDVFNNPIRIDSSWYPANIDYVLPKWQNFLIEFVSDQNYTGLCVETKNRLLNESFQSFEFVIEFARNVRKTNNIQEVVIKIKDIQTVKMFNESGCDMGQSSRCTVSSDYAEKWAYDVSVEIMKNVSSNSLSLQSDIYEKVLQYLCKDKVPIESVGVKRL----KKIDKDELACQIPQNMTQ 308
BLAST of SMED30007360 vs. TrEMBL
Match: A0A1P8CUY3 (Estrella/neura-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 107.842 bits (268), Expect = 2.121e-23 Identity = 67/232 (28.88%), Postives = 117/232 (50.43%), Query Frame = 1 Query: 304 IPIIFWNYSYLQKINNLKYLMTTDTNITTK---ILPPTGVSSFQINWRRPGKS--SEHIELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKRLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIMLSDFEIEPIL---SCKNGYDSNCYIFLNSAKRFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIPKHFIQ 975 +PIIFW+ SY+ + N M+ N ++ ILPP+G S+++NWRR + I +D+S+ + ++Y LP F++EF LC E KN L+ E Q E V+ F I + I + D + + C G S C + + A++++Y +++ ++K +S +++LQ IY + L LC KV S+ +R+ + ++ L C IP++ Q Sbjct: 2 MPIIFWDDSYMTNVANSIQKMSKLKNFVSEKPFILPPSGFRSYRLNWRRDLNDVFNNPIRIDSSWYPANIDYVLPKWQNFLIEFVSDQNYTGLCVETKNRLLNESFQSFEFVIEFARNVRKTNNIQEVVIKIKDIQTVKMFNESGCDMGQSSRCTVSSDYAEKWAYDVSVEIMKNVSSNSLSLQSDIYEKVLQYLCKDKVPIESVGVKRL----KKIDKDELACQIPQNMTQ 229
BLAST of SMED30007360 vs. Planmine SMEST
Match: SMESG000013666.1 (SMESG000013666.1) HSP 1 Score: 634.41 bits (1635), Expect = 0.000e+0 Identity = 310/317 (97.79%), Postives = 314/317 (99.05%), Query Frame = 1 Query: 121 GKTQSKFPPTIQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKINNLKYLMTTDTNITTKILPPTGVSSFQINWRRPGKSSEHIELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKRLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIMLSDFEIEPILSCKNGYDSNCYIFLNSAKRFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIPKHFIQYIDRRISDKTIFTIDNLLSNVKEHEYSIPIFN 1071 GKTQSKFPPTIQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKINNLKYLMTTDTNITTKILPPTGVSSFQINWRRPGKSSEH+ELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSK+LCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINI LSDFEIEPILSC+NGYDSNCYI+LNSAK FSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIPKHFIQYIDRRISDKTIFTID LLSNVKEHEYSIPIFN Sbjct: 17 GKTQSKFPPTIQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKINNLKYLMTTDTNITTKILPPTGVSSFQINWRRPGKSSEHMELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKQLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIKLSDFEIEPILSCRNGYDSNCYIYLNSAKTFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIPKHFIQYIDRRISDKTIFTIDTLLSNVKEHEYSIPIFN 333
BLAST of SMED30007360 vs. Planmine SMEST
Match: SMESG000041686.1 (SMESG000041686.1) HSP 1 Score: 146.747 bits (369), Expect = 2.621e-38 Identity = 84/282 (29.79%), Postives = 153/282 (54.26%), Query Frame = 1 Query: 151 IQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKI------NNLKYLMTTDTNITTKILPPTGVSSFQINWRR---PGKSSEHIELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKRLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIMLSDFEIEPIL---SCKNGYDSNCYIFLNSAKRFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIP 960 ++VN+S+ Y KL I SA PL +LDV L+ + C++N + + L IP+IFW Y+++I NL T D ILPP+GV +F+I WRR + +I LDTS+Q + +Y LPM H+F++EF++ K+LC + KN L+ + +Q E+ ++F+ + IP I I +++ + +CK + C++ A+++SY ++ +++ + ++NLQ I+ + LC ++ I + V ++ ++++ + C IP Sbjct: 335 VRVNISDFVYKKLMISKDNILSASPLALLDVKLLKKYHCYKNIRSSLWALPIPVIFWTDLYMERIMASVKKGNLLRNFTGDK---VSILPPSGVIAFRITWRRNYIENSENTNISLDTSWQDTQTDYVLPMRHSFLMEFSDNGDGKKLCVDTKNKLLNDSMQISEMTIDFQKTAGRKRNIPDIQIKINNIRSVKMYNESNCKTQSEDKCFVSKEFAEKWSYDVSTRMMENVEIIHLNLQKDIFDAVFEYLCKNRMSIFEIELKTVRRLSKGIDNE-MACKIP 612
BLAST of SMED30007360 vs. Planmine SMEST
Match: SMESG000041686.1 (SMESG000041686.1) HSP 1 Score: 146.747 bits (369), Expect = 2.745e-38 Identity = 84/282 (29.79%), Postives = 153/282 (54.26%), Query Frame = 1 Query: 151 IQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKI------NNLKYLMTTDTNITTKILPPTGVSSFQINWRR---PGKSSEHIELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKRLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIMLSDFEIEPIL---SCKNGYDSNCYIFLNSAKRFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIP 960 ++VN+S+ Y KL I SA PL +LDV L+ + C++N + + L IP+IFW Y+++I NL T D ILPP+GV +F+I WRR + +I LDTS+Q + +Y LPM H+F++EF++ K+LC + KN L+ + +Q E+ ++F+ + IP I I +++ + +CK + C++ A+++SY ++ +++ + ++NLQ I+ + LC ++ I + V ++ ++++ + C IP Sbjct: 343 VRVNISDFVYKKLMISKDNILSASPLALLDVKLLKKYHCYKNIRSSLWALPIPVIFWTDLYMERIMASVKKGNLLRNFTGDK---VSILPPSGVIAFRITWRRNYIENSENTNISLDTSWQDTQTDYVLPMRHSFLMEFSDNGDGKKLCVDTKNKLLNDSMQISEMTIDFQKTAGRKRNIPDIQIKINNIRSVKMYNESNCKTQSEDKCFVSKEFAEKWSYDVSTRMMENVEIIHLNLQKDIFDAVFEYLCKNRMSIFEIELKTVRRLSKGIDNE-MACKIP 620
BLAST of SMED30007360 vs. Planmine SMEST
Match: SMESG000026791.1 (SMESG000026791.1) HSP 1 Score: 140.584 bits (353), Expect = 9.515e-38 Identity = 84/283 (29.68%), Postives = 144/283 (50.88%), Query Frame = 1 Query: 151 IQVNLSNVAYVKLEQISSKIFSAPPLVILDVVKLRNFTCFRNFYTMKYTLGIPIIFWNYSYLQKINNLKYLMTTDTNITTK---ILPPTGVSSFQINWRRPGKS--SEHIELDTSFQRSYLNYFLPMSHTFIVEFNEGNTSKRLCNEIKNSLITEPIQRQELVVNFKGIKTIEEKIPSINIMLSDFEIEPIL---SCKNGYDSNCYIFLNSAKRFSYLLAINVLKRMSKYNINLQMQIYSRALDELCGIKVIYSSISNQRVVGTAEVVNSQILVCSIPKHFIQ 975 +QVN+SN+ Y +L + +A PL ILDV L+ C++N T ++ +PIIFW+ SY+ + N M+ N ++ ILPP+G S+++NWRR + I +D+S+ + ++Y LP F++E LC E KN L+ E Q E V+ F I + I + D + + C G S C + + A++++Y +++ ++K S +++LQ IY + L LC KV S+ +R+ + ++ L C IP++ Q Sbjct: 30 VQVNISNIIYKQLMTTKDNVLAASPLAILDVNLLKKHKCYKNIRTNSWSFMMPIIFWDDSYMTNVANSIQKMSKLKNFVSEKPFILPPSGFRSYRLNWRRDLNDVFNNPIRIDSSWYPANIDYVLPKWQNFLIELVSDQNYTGLCVETKNRLLNESFQSFEFVIEFARNVRKTNNIQEVVIKIKDIQTVKMFNESGCDMGQSSRCTVSSDYAEKWAYDVSVEIMKNFSSNSLSLQSDIYEKVLQYLCKDKVPIESVGVKRL----KKIDKDELACQIPQNMTQ 308 The following BLAST results are available for this feature:
BLAST of SMED30007360 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30007360 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30007360 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of SMED30007360 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30007360 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30007360 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007360 ID=SMED30007360|Name=SMED30007360|organism=Schmidtea mediterranea sexual|type=transcript|length=1247bpback to top protein sequence of SMED30007360-orf-1 >SMED30007360-orf-1 ID=SMED30007360-orf-1|Name=SMED30007360-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=334bp MKTLLLVLVIIIYTLTGKTQSKFPPTIQVNLSNVAYVKLEQISSKIFSAPback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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