SMED30007348

Overview
NameSMED30007348
Smed IDSMED30007348
Length (bp)1712
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007348 (SMED30007348) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007348 (SMED30007348) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007348 (SMED30007348) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007348 (SMED30007348) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007348

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30007348SMESG000002022.1 Contig20newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30007348SMESG000002945.1 Contig20newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30007348SMESG000043828.1 dd_Smed_v4_10959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007348SMESG000043828.1 dd_Smed_v4_10959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007348 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001891:12834..14545 +8362
Homology
BLAST of SMED30007348 vs. Planmine SMEST
Match: SMESG000043828.1 (SMESG000043828.1)

HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0
Identity = 402/407 (98.77%), Postives = 404/407 (99.26%), Query Frame = 2
Query:  203 GREIYFRPATFKSVDIRNEKMFIISNVVWGLLALVYPLTKIXXXXXXXXXXXXXXXXXXFLIIWLLMFYNKVPVTPSQNCTYRWAYVLNAVSVIYYTSSEMVWNINHKITQNSTNNGNCNSSIKDFLKGNLPNSLFETFFYSFCXXXXXXXXXXXXXXXFANQHYSSARKPAFYNFKSVKIRQFKDVIFGLLVSFGLISFTIYTLIENSKPYHCDTLIVYFYINSATYIIGIAASVALLFEFRKWKFMEHILSEIDRQILELGTIGQMIYAXXXXXXXXXXXXYQAHGDKQLLISSLICYILMILDLCVQRTTIIQILKLHSSGIVQQESNSKILLQICPGMILMTLNLCMIPTSFYFIVYPTDLIVLHSDLNPDLWIVFKYAKWPVMLLHRLHGTIVYGYYLYQTW 1423
            GREIYFRPATFKSVDIRNEKMFIISN VWGLLALVYP+TKIYLYN NVVVLSVRIVHIVFLIIWLLMFYNKVPVTPSQNCTYRWAYVLNAVSVIYYTSSEMVWNINHKITQNSTNNGNCNSSIKDFLKGNLPNSLFETFFYSFCLLS+VLLLNRLTILLFANQHYSSARKPAFYNFKSVKIRQFKDVIFGLLVSFGLISFTIYTLIENSKPYHCDTLIVYFYINSATYIIGIAASVALLFEFRKWKFMEHILSEIDRQILELGTIGQMIYAVSIVISTVIVLSYQAH DKQLLISSLICYILMILDLCVQRTTIIQILKLHSSGIVQQESNSKILLQICPGMILMTLNLCMIPTSFYFIVYPTDLIVLHSDLNPDLWIVFKYAKWPVMLLHRLHGTIVYGYYLYQTW
Sbjct:    5 GREIYFRPATFKSVDIRNEKMFIISNAVWGLLALVYPITKIYLYNCNVVVLSVRIVHIVFLIIWLLMFYNKVPVTPSQNCTYRWAYVLNAVSVIYYTSSEMVWNINHKITQNSTNNGNCNSSIKDFLKGNLPNSLFETFFYSFCLLSVVLLLNRLTILLFANQHYSSARKPAFYNFKSVKIRQFKDVIFGLLVSFGLISFTIYTLIENSKPYHCDTLIVYFYINSATYIIGIAASVALLFEFRKWKFMEHILSEIDRQILELGTIGQMIYAVSIVISTVIVLSYQAHLDKQLLISSLICYILMILDLCVQRTTIIQILKLHSSGIVQQESNSKILLQICPGMILMTLNLCMIPTSFYFIVYPTDLIVLHSDLNPDLWIVFKYAKWPVMLLHRLHGTIVYGYYLYQTW 411          
BLAST of SMED30007348 vs. Planmine SMEST
Match: SMESG000043633.1 (SMESG000043633.1)

HSP 1 Score: 137.502 bits (345), Expect = 7.914e-35
Identity = 124/428 (28.97%), Postives = 210/428 (49.07%), Query Frame = 2
Query:  209 EIYFRPATFKSVDIR-NEKMFIISNVVWGLLALVYPLTKIXXXXXXXXXXXXX--------XXXXXFLIIWLLMFYNKVPVTPSQNCT-------YRWAYVLNAVSVIYYTSSEMVWNINHKITQNSTNNGNCNSSIKDF----LKGNLPNSLFETFFYSFCXXXXXXXXXXXXXXXFANQHYSSARKPAFY--NFKSVKIRQFKDVIFGLLVSFGL-ISFTIYTLIENSKPYHCDTLIVYFYINSATYIIGIAASVALLFEFRKWKFMEHILSEIDRQILELGTIGQMIYAXXXXXXXXXXXXYQAHGDKQLLISSLICYILMILDLCVQRTTIIQILKLHSSGIVQQESNSKILLQICPGMILMTLNLCMIPTSFYFIVYPTDLIVLHSDLNPDLWIVFKYAKWPVMLLHRLHGTIVYGYYLYQTW 1423
            +I  R A +  V +R N  +F + N +WG+L++ Y + KI         L V         +++ + L  WL+  + K+P     NC        Y++A +LN V + Y    E + + +  +   S  + N  +  ++F    L+      +FE+FF SF   +L+L++N    L   +       KP FY    +  K +  ++ + G  +S  + I F    +     P     +++Y   N +  ++GI AS   ++E ++W+F   I S+ID  ILELGT  +++ A S     +     +  G   L    +I Y ++I+ LC+QR  IIQ+LKL    +   +S S  + Q+CP MILMTLN   +PT+++F ++P       + LN   W+++K  K P+ +LHR+HG IV+GY LY  W
Sbjct:   58 DIKHRRAEYDEVTLRTNTHIFYVINFLWGVLSVSYFIWKIIEIIQTSTKLLVLKVWIISVRMIYFLLLTFWLIYSFKKLP-----NCQKRIEYIGYKFAIILNIVFLSYIFCHEKMRSASDSLKIPSKEH-NTTAKCQNFHGIPLQMCQNQEVFESFFDSFLFTTLLLVVNLYETLTSTSTF--RKIKPEFYIKRNEGNKTKPARERLLGFFISMIITILFLTSIVYFYLHPKEDKAILLYICSNLSINVLGIIASFFFIYEMQEWQFFSAISSKIDLYILELGTANKLLMASSSAFVLLFFS--EKQGISSLYALEMINYSVLIIQLCLQRVLIIQLLKLSHRHL--PKSKSIFITQLCPCMILMTLNFFYVPTTYFFNLHPITHKATKTILNNYTWVIYKTFKTPISILHRMHGAIVFGYILYHMW 473          
BLAST of SMED30007348 vs. Planmine SMEST
Match: SMESG000079760.1 (SMESG000079760.1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.028e-6
Identity = 51/196 (26.02%), Postives = 98/196 (50.00%), Query Frame = 2
Query:  845 DTLIVYFYINSATYIIGIAASVALLFEFRKW-KFMEHILSEIDRQILELGTIGQMIYAXXXXXXXXXXXXYQAHGDKQLLISSLICYILMILDLCVQRTTIIQILKLHSSGIVQQESNSKILLQICPGMILMTLNLCMIPTSFYFIVYPTDLIVLHSDLNPDLWIVFKYAKWPV--MLLHRLHGTIVYGYYLYQTW 1423
            + LIV+    +  YI+G+  SV  + E R W K +E+    +D+ +L LG  GQ+++A+  +   V     Q+       +   IC I +I+ + +Q      ++ L +S  +Q+ SN  +  ++CP + L+ LNL  +  + Y  + P   +    D++ DL+  F    + +   L+HRLH T+ +  +L + +
Sbjct:  296 NALIVFSSCATVIYIVGMILSVIYIHEMRNWEKEIENNKYILDKFLLWLGCFGQIVFAIVQISMMV-----QSDNRNGTTVIMCICSIFLIIQISLQNI----VVNLIASRKIQRPSNESLFGKVCPPVGLLGLNLGCLLITIY--LSPLSNVFSFGDMSVDLF-HFGLHIFAICFTLIHRLHATVFFAIFLSKIY 479          
The following BLAST results are available for this feature:
BLAST of SMED30007348 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007348 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000043828.10.000e+098.77SMESG000043828.1[more]
SMESG000043633.17.914e-3528.97SMESG000043633.1[more]
SMESG000079760.11.028e-626.02SMESG000079760.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007348 ID=SMED30007348|Name=SMED30007348|organism=Schmidtea mediterranea sexual|type=transcript|length=1712bp
TGGACTTTGCCTTTTCAATCAAATCATTATCATTTGAAAAATGTTATTCA
TAATACTACAATGGATTGGAAAGGGTTCCTTCATTTTCTATACAATAATA
GCCTTCATTGCTATATTTTATCAAGTGGGTAAAAAATGGCAAGAATTATA
TCTATTAGAGCTCTTATTTTCAATCTTGTCGATCTTGTCCATGATTCTCA
TCGGAAGAGAGATTTATTTCCGGCCGGCAACTTTTAAATCCGTCGATATT
CGGAACGAAAAAATGTTTATAATATCAAACGTCGTCTGGGGATTATTAGC
GCTTGTGTATCCCTTAACGAAAATATATCTATACAATTACAATGTCGTCG
TCCTCTCAGTGAGAATCGTTCACATCGTCTTTCTAATAATTTGGCTTTTG
ATGTTCTACAACAAGGTTCCGGTGACCCCCTCTCAGAATTGCACTTACCG
TTGGGCATACGTATTAAATGCGGTATCGGTCATTTACTATACATCGAGTG
AAATGGTTTGGAATATTAATCATAAAATCACACAAAACTCGACAAATAAT
GGCAATTGCAATTCGAGTATAAAGGATTTCTTAAAAGGCAACCTTCCAAA
TAGCCTTTTTGAAACTTTCTTTTATTCGTTCTGTTTACTATCTTTAGTGT
TGCTGTTGAATCGTTTGACGATCCTTCTATTCGCCAATCAGCATTATTCT
TCAGCGCGAAAACCGGCATTCTATAATTTTAAATCAGTGAAAATTCGACA
GTTTAAAGACGTGATTTTTGGGCTGTTAGTGAGCTTTGGGTTGATTTCTT
TCACGATTTATACTTTGATAGAAAATTCCAAGCCATATCATTGTGATACG
TTAATCGTTTATTTTTATATCAATTCCGCAACATACATCATTGGAATTGC
AGCGTCGGTGGCGTTGTTATTCGAATTTAGGAAATGGAAATTCATGGAGC
ACATTTTATCGGAAATCGACAGACAGATTTTGGAACTTGGAACTATCGGT
CAAATGATCTACGCCGTGAGCATTGTCATTTCAACGGTTATTGTACTCAG
CTATCAAGCTCATGGGGATAAACAGCTTTTGATCAGTTCTCTGATTTGTT
ATATACTAATGATCCTTGATCTCTGCGTTCAAAGAACGACTATTATTCAA
ATCTTGAAACTTCATTCGAGTGGAATAGTACAACAGGAAAGCAACAGTAA
AATTCTCCTACAAATTTGTCCAGGCATGATTTTAATGACTTTAAATTTAT
GTATGATTCCGACGAGTTTTTATTTTATAGTTTACCCGACTGATTTAATC
GTTCTCCATTCGGATTTGAATCCGGATTTGTGGATTGTATTCAAATATGC
CAAATGGCCGGTAATGCTTCTTCACCGACTTCATGGGACTATTGTTTATG
GATATTACCTTTATCAAACTTGGTGATTCTTTCAATAGATTTCATTCACT
TTTTAATAAAACCCCTATAATTGTCATACTATGTATCACACAGTGCAAAT
CAAACGATAAAGAGATGTTTCATCAATAAATCAAAATTAAATACAAATTT
CGGTTTCATTTAAACAAAAATTGATCACTTTATTAAATATATTGTTGGGG
CTGTTGGTGAATTTAAATAGGATGTATTGTCAATCTGACTCCAAGCGTGC
TTTTAAACAACAGTAATGAACTCCAAACGATATAAACGTTATTGATTAAT
ACACTTTATCTC
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protein sequence of SMED30007348-orf-1

>SMED30007348-orf-1 ID=SMED30007348-orf-1|Name=SMED30007348-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=462bp
MLFIILQWIGKGSFIFYTIIAFIAIFYQVGKKWQELYLLELLFSILSILS
MILIGREIYFRPATFKSVDIRNEKMFIISNVVWGLLALVYPLTKIYLYNY
NVVVLSVRIVHIVFLIIWLLMFYNKVPVTPSQNCTYRWAYVLNAVSVIYY
TSSEMVWNINHKITQNSTNNGNCNSSIKDFLKGNLPNSLFETFFYSFCLL
SLVLLLNRLTILLFANQHYSSARKPAFYNFKSVKIRQFKDVIFGLLVSFG
LISFTIYTLIENSKPYHCDTLIVYFYINSATYIIGIAASVALLFEFRKWK
FMEHILSEIDRQILELGTIGQMIYAVSIVISTVIVLSYQAHGDKQLLISS
LICYILMILDLCVQRTTIIQILKLHSSGIVQQESNSKILLQICPGMILMT
LNLCMIPTSFYFIVYPTDLIVLHSDLNPDLWIVFKYAKWPVMLLHRLHGT
IVYGYYLYQTW*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 270..292
NoneNo IPR availableTMHMMTMhelixcoord: 241..260
NoneNo IPR availableTMHMMTMhelixcoord: 75..97
NoneNo IPR availableTMHMMTMhelixcoord: 313..335
NoneNo IPR availableTMHMMTMhelixcoord: 345..363
NoneNo IPR availableTMHMMTMhelixcoord: 38..60
NoneNo IPR availableTMHMMTMhelixcoord: 392..414
NoneNo IPR availableTMHMMTMhelixcoord: 193..215
NoneNo IPR availableTMHMMTMhelixcoord: 4..26
NoneNo IPR availableTMHMMTMhelixcoord: 104..123