DUF862 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007270 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A164QG11 (DUF862 domain-containing protein OS=Daphnia magna OX=35525 GN=APZ42_028510 PE=4 SV=1) HSP 1 Score: 66.6254 bits (161), Expect = 1.661e-9 Identity = 39/121 (32.23%), Postives = 64/121 (52.89%), Query Frame = 1 Query: 307 LVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKS----MKELVAHXXXXXXXXXXXXXXNLHNCKGFAD 657 LVD+T+ I V YYK PL + QL + +HAF++ +T ++S+EKND G+ +Q+S++ V + +R + VK S + EL+ HI + + +NC+ FAD Sbjct: 50 LVDVTERIIEVRYYKHPLGSWQLMDNVLHHAFIVFETNAWWWSIEKNDEGVTIQRSKDFDSVCHRYRRTKRKTSIGKGVEMVKKASGSATVMELINHIWRKNYLNQEYNFLT-YNCQHFAD 169
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A3M6TN79 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00005107 PE=4 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 1.177e-8 Identity = 39/121 (32.23%), Postives = 65/121 (53.72%), Query Frame = 1 Query: 307 LVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNV---SDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFADR 660 L+D ++ I N+W +K+PLS Q TQL H F+++KT+ ++S+EKN IL+Q+S++ V+ +R V K+MK+L+ E + N++NCK FA + Sbjct: 68 LIDFSENILNIWVFKSPLSDWQRTQLFLNHQFVVIKTKNWWWSIEKNSEVILIQRSKHFKGYGGVLDCANNNKRKTPLLRVSSDVGQKTMKDLLEF-LHEEDELNKTYFWNVNNCKHFAKK 187
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A164JSN2 (Uncharacterized protein OS=Daphnia magna OX=35525 GN=APZ42_000269 PE=4 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 1.414e-7 Identity = 37/123 (30.08%), Postives = 65/123 (52.85%), Query Frame = 1 Query: 307 LVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQR----NAVTKLNEKKVK---NKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFA 654 L+D + I Y PLS+LQ T + YHAF++++T ++S+EKN GI +Q+S+N+ V + K+R ++T + K N ++ EL+ ++ + + NC+GFA Sbjct: 47 LIDTEERIQKASVYSNPLSSLQATNALLYHAFIVIETNDWWWSIEKNTEGITIQRSKNLKSVRDMYQRKKRTTGWTSLTSIRNNKTAQGGNTTISELINYVWRKDCVTNVYHVLS-ANCQGFA 168
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: E9GQZ7 (Uncharacterized protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_305262 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 1.879e-7 Identity = 26/80 (32.50%), Postives = 51/80 (63.75%), Query Frame = 1 Query: 280 WSYGTEIRYLVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQR 519 W +I L D ++ I V YY PL+ Q+++ + +HAF++L+TE ++S+EK++ GI +Q+S+++ +V+ + R Sbjct: 37 WELKEKIENLPDSSEKINEVRYYTHPLNWWQMSKYVLHHAFIVLETESWWWSIEKDEGGITIQRSKHLENVLDQCRRHGR 116
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: E9GQ32 (Uncharacterized protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_320721 PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 4.188e-7 Identity = 39/119 (32.77%), Postives = 67/119 (56.30%), Query Frame = 1 Query: 328 ITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQRNAVTKLN------EKK---VKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFAD 657 I V YY PL +Q+TQL+ +HAF++ TE ++S+E+N+ G+ +Q+S+ + V K ++++ T L EK+ + N ++KEL+ HI + E +NCK FA+ Sbjct: 54 ILEVRYYTNPLYGVQVTQLVLHHAFIVFLTERAWWSIERNELGVTIQRSKKIEFVRDKF--RRQDRTTDLFGSGIHWEKRCFGITNVTLKELLTHICREDYLNEDYDLLE-NNCKHFAE 169
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024772.1 (SMESG000024772.1) HSP 1 Score: 114.775 bits (286), Expect = 3.077e-32 Identity = 72/73 (98.63%), Postives = 73/73 (100.00%), Query Frame = 1 Query: 463 MQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFADRALKLLDS 681 MQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHIEESEIGIEYISSSN+HNCKGFADRALKLLDS Sbjct: 1 MQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHIEESEIGIEYISSSNIHNCKGFADRALKLLDS 73
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024661.1 (SMESG000024661.1) HSP 1 Score: 95.1301 bits (235), Expect = 1.234e-23 Identity = 53/140 (37.86%), Postives = 85/140 (60.71%), Query Frame = 1 Query: 250 NLSANSDTYSWSYGTEIRYLVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFADRALK 669 N SA +D + G+E+R L+D ++ I W YK L Q+T YHAF+I +T+ Y+S+EKN SGI +QKS++ +++V+K G +RN+ T L+ + ++ EL+ +E +E+ Y NC+GFADRA + Sbjct: 23 NTSAPNDVFK---GSEMRKLIDTSETIIQAWKYKQKLVWFQITSFFLYHAFVIFETKDWYWSIEKNGSGIWIQKSKSKNELVTKNGGNERNSTTLLDSEFGNFITIGELIEWVENNELINRYFYIETYFNCQGFADRAYQ 159
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024663.1 (SMESG000024663.1) HSP 1 Score: 94.3597 bits (233), Expect = 1.910e-23 Identity = 52/138 (37.68%), Postives = 87/138 (63.04%), Query Frame = 1 Query: 250 NLSANSDTYSWSYGTEIRYLVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFADRA 663 N SA ++ ++ G+E+R L+D ++ I VW YK L Q+T YHAF+I +T Y+S+EKN SGI +QKS++ ++V+K+ G +RN+ T L+ + + ++K ++ IE+ E+ Y ++ NC+GFADR Sbjct: 23 NTSAPNEVFN---GSEMRKLIDTSETIVKVWKYKEKLVWWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDNLVAKIGGNERNSTTLLDSCQGRFVTIKNIIEWIEKEELKSAYWYMNSDDNCQGFADRG 157
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024649.1 (SMESG000024649.1) HSP 1 Score: 92.4337 bits (228), Expect = 1.139e-22 Identity = 51/134 (38.06%), Postives = 86/134 (64.18%), Query Frame = 1 Query: 286 YGTEIRYLVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFADRALKLLDS*F 687 YG++++ + +L++ I VW YK PL + QLT +IFYH FL+L+T+ +S+EKN +GI +QKS N ++++ K G +R +V+ L + +K L I ++E+ I+Y + +NCKGF +RA K + + F Sbjct: 28 YGSQMKQMGNLSERIQQVWRYKEPLKSWQLTSVIFYHIFLVLQTDKWNWSIEKNGTGIWVQKSENHNELLKKKGGVERKSVSLLGHRIGNFVRIKSLFDWIIKNELKIKYYWLDSYYNCKGFVERAFKFITNTF 161
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024669.1 (SMESG000024669.1) HSP 1 Score: 89.7373 bits (221), Expect = 1.044e-21 Identity = 49/125 (39.20%), Postives = 77/125 (61.60%), Query Frame = 1 Query: 289 GTEIRYLVDLTKAITNVWYYKTPLSALQLTQLIFYHAFLILKTEGHYYSLEKNDSGILMQKSRNVSDVVSKVEGKQRNAVTKLNEKKVKNKSMKELVAHXXXXXXXXXXXXXXNLHNCKGFADRA 663 G E++ DL+K I +VW YKTP+ +QLT FYH F++++T+ ++S+EKN+ GI +QK R +DV+SK+ G++RN V L + + + LV IE +E+ Y + N KGF D+ Sbjct: 27 GGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKV 151 The following BLAST results are available for this feature:
BLAST of DUF862 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of DUF862 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DUF862 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007270 ID=SMED30007270|Name=DUF862 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=912bpback to top protein sequence of SMED30007270-orf-1 >SMED30007270-orf-1 ID=SMED30007270-orf-1|Name=SMED30007270-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=175bp MSFGSQSNSKSTGSSRSNCPSTSGDSRSTANLSANSDTYSWSYGTEIRYLback to top Annotated Terms
The following terms have been associated with this transcript:
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