SMED30007172

Overview
NameSMED30007172
Smed IDSMED30007172
Length (bp)947
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007172 (SMED30007172) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007172 (SMED30007172) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007172 (SMED30007172) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007172 (SMED30007172) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007172

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30007172SMESG000020303.1 SmedASXL_009077SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007172 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002047:73128..74263 +4626
Homology
[Close]
BLAST of SMED30007172 vs. Ensembl Human
Match: MPHOSPH10 (M-phase phosphoprotein 10 [Source:HGNC Symbol;Acc:HGNC:7213])

HSP 1 Score: 53.5286 bits (127), Expect = 2.631e-7
Identity = 55/206 (26.70%), Postives = 96/206 (46.60%), Query Frame = 1 
Query:  172 KKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVN---FANHVRKVKPTESQ---TNEIVISGEKSKLSLADEYVNIMKREEDFLKL--------QGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLD 747
            K W ++GEV A    +N   E  ++      +H V      V  +E    +E+ +++ +    + + VRK KP E        + +  EKSKLSLA+ Y      E++++KL        +  +    +K M SLF++LD L + ++IP     E+KV+  N      EE+    + +   LAP+E+    K  ++K   E++  D
Sbjct:  382 KPWQLQGEVTAQKRPENSLLEETLH-----FDHAVRMAP--VITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIY------EQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVS-NLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATD 573
[Close]
BLAST of SMED30007172 vs. Ensembl Fly
Match: CG13097 (gene:FBgn0032051 transcript:FBtr0079697)

HSP 1 Score: 54.6842 bits (130), Expect = 6.214e-8
Identity = 63/238 (26.47%), Postives = 106/238 (44.54%), Query Frame = 1 
Query:  172 KKWFMKGEVFAS---------DTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDAT----------RKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ*IILTAL 828
            K W +KGEV A+         + +D ++   P     E  N  +     +   D+    +E  V + VN     RK         ++V+  EKSK SL+  Y    +RE + L    D  D T          ++ M+SLF++LD L + ++ P   A E+K++  N      EE+    + +   LAP+E+    K + + G TER + D+ R+++        KK ++Q  I +AL
Sbjct:  362 KPWQLKGEVKAANRPQNSLLEEILDFDSTTRPTAPITEEDNRSIEDIIKQRIRDKAWDDVERTV-RPVNTPQEYRK---------QLVLDQEKSKQSLSQIYEAQYQREMEKLDPNRDADDKTGPEPKEHQEIKRAMRSLFLKLDALSNFHFTPKPVAPEIKIVT-NTPAVHMEEVAPLAVSDAKLLAPEEVFRGPKHAPL-GKTERDRTDKNRERR--------KKKQKQRAIHSAL 579
[Close]
BLAST of SMED30007172 vs. Ensembl Mouse
Match: Mphosph10 (M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) [Source:MGI Symbol;Acc:MGI:1915223])

HSP 1 Score: 55.0694 bits (131), Expect = 6.232e-8
Identity = 56/208 (26.92%), Postives = 96/208 (46.15%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVN---FANHVRKVKPTESQ---TNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDA--------TRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLD 747
            D K W ++GEV A    +N   E  ++      +H V      V  +E   ++E+ +++ +    + +  RK KP E        + +  EKSKLSLA+ Y      E+++LKL     +          +K M SLF++LD L + ++IP     E+KV+  N      EE+    + +   LAP+E+    K  ++K   E++  D
Sbjct:  381 DKKPWQLQGEVTAQKRPENSLLEETLH-----FDHAVRMAP--VITEETTLHLEDIIKQRIRDQAWDDVERKEKPKEDAYEYKKRLTLDHEKSKLSLAEIY------EQEYLKLNQQKTEEEDNPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVS-NLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDLKTAAEKTATD 574
[Close]
BLAST of SMED30007172 vs. UniProt/SwissProt
Match: sp|Q810V0|MPP10_MOUSE (U3 small nucleolar ribonucleoprotein protein MPP10 OS=Mus musculus OX=10090 GN=Mphosph10 PE=1 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 4.359e-7
Identity = 56/208 (26.92%), Postives = 96/208 (46.15%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVN---FANHVRKVKPTESQ---TNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDA--------TRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLD 747
            D K W ++GEV A    +N   E  ++      +H V      V  +E   ++E+ +++ +    + +  RK KP E        + +  EKSKLSLA+ Y      E+++LKL     +          +K M SLF++LD L + ++IP     E+KV+  N      EE+    + +   LAP+E+    K  ++K   E++  D
Sbjct:  381 DKKPWQLQGEVTAQKRPENSLLEETLH-----FDHAVRMAP--VITEETTLHLEDIIKQRIRDQAWDDVERKEKPKEDAYEYKKRLTLDHEKSKLSLAEIY------EQEYLKLNQQKTEEEDNPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVS-NLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDLKTAAEKTATD 574
[Close]
BLAST of SMED30007172 vs. UniProt/SwissProt
Match: sp|O00566|MPP10_HUMAN (U3 small nucleolar ribonucleoprotein protein MPP10 OS=Homo sapiens OX=9606 GN=MPHOSPH10 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.264e-6
Identity = 55/206 (26.70%), Postives = 96/206 (46.60%), Query Frame = 1 
Query:  172 KKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVN---FANHVRKVKPTESQ---TNEIVISGEKSKLSLADEYVNIMKREEDFLKL--------QGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLD 747
            K W ++GEV A    +N   E  ++      +H V      V  +E    +E+ +++ +    + + VRK KP E        + +  EKSKLSLA+ Y      E++++KL        +  +    +K M SLF++LD L + ++IP     E+KV+  N      EE+    + +   LAP+E+    K  ++K   E++  D
Sbjct:  382 KPWQLQGEVTAQKRPENSLLEETLH-----FDHAVRMAP--VITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIY------EQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVS-NLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATD 573
[Close]
BLAST of SMED30007172 vs. TrEMBL
Match: H2KSR1 (U3 small nucleolar RNA-associated protein MPP10 (Fragment) OS=Clonorchis sinensis OX=79923 GN=CLF_109974 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 7.114e-12
Identity = 62/206 (30.10%), Postives = 103/206 (50.00%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDF-DATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQ----EEIGVAGIPNLLKLAPQELL-PNKKISEMKGTTERSKLDRKRKKK 765
            D K W++KGEV A D  +N   E  ++     +  H        + D+I+ +I++A+ K  +F + V K K   SQ+    +     + SL ++Y N+  ++    K Q D   ++ + EM+ LF +LD+L H +++P   + E  VL     QS Q    EE G A       LAP+E+  P  ++  +KG TE +  DR+R +K
Sbjct:   14 DDKPWYLKGEVVAQDRAENTVLEEYLD-----VQRHGKFKPPPADEDKILEFIKKAI-KEQSFDSPVFKCKEKTSQSTSQAVPITAQQKSLVEDYENLFAKKNLLEKEQEDPVKNSIQAEMRELFEKLDSLSHLHFVPYKHSPEATVL-----QSKQAMVMEEAGPAAASTADLLAPEEVFAPRGEV--LKGATELTSTDRRRHRK 206
[Close]
BLAST of SMED30007172 vs. TrEMBL
Match: A0A1S8WRH1 (Mpp10 protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_07199 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.007e-11
Identity = 63/208 (30.29%), Postives = 107/208 (51.44%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKV--KPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDF-DATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQ----EEIGVAGIPNLLKLAPQELL-PNKKISEMKGTTERSKLDRKRKKK 765
            D K W++KGEV A D  +N   E  ++     +  H        + D+I+ +I++A+ K  +F + V K   KP++S    + I+ ++   SL ++Y N+  ++    K Q D   ++ + EM+ LF +LD+L H +++P   + E  VL     QS Q    EE G A       LAP+E+  P  ++  +KG TE +  DR+R +K
Sbjct:   22 DDKPWYLKGEVVAQDRAENTVLEEYLD-----VQRHGKFKPPPADEDKILEFIKKAI-KEQSFDSPVFKCKEKPSQSTGQAVPITAQQK--SLVEDYENLFAKKNLLEKEQEDPVKNSIQAEMRELFEKLDSLSHLHFVPYKHSPEATVL-----QSKQAMVMEEAGPAATSTADLLAPEEVFAPRGEV--LKGATELTSTDRRRHRK 214
[Close]
BLAST of SMED30007172 vs. TrEMBL
Match: A0A4S2LGT4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007309 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.096e-11
Identity = 63/206 (30.58%), Postives = 104/206 (50.49%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDF-DATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQ----EEIGVAGIPNLLKLAPQELL-PNKKISEMKGTTERSKLDRKRKKK 765
            D K W++KGEV A D  +N   E  ++     +  H        + D+I+ +I++A+ K  +F + V K K   SQ+   V+     + SL ++Y N+  ++    K Q D   ++ + EM+ LF +LD+L H +++P   + E  VL     QS Q    EE G A       LAP+E+  P  ++  +KG TE +  DR+R +K
Sbjct:   12 DDKPWYLKGEVVAQDRAENTVLEEYLD-----VQRHGKFKPPPADEDKILEFIKKAI-KEQSFDSPVFKRKEKTSQSTSQVVPITAQQKSLVEDYENLFAKKNLLEKEQEDPVKNSIQAEMRELFEKLDSLSHLHFVPYKHSPEATVL-----QSKQAMVMEEAGPAAASTADLLAPEEVFAPRGEV--LKGETELTSTDRRRHRK 204
[Close]
BLAST of SMED30007172 vs. TrEMBL
Match: A0A4S2LN98 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007309 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.373e-11
Identity = 63/206 (30.58%), Postives = 104/206 (50.49%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDF-DATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQ----EEIGVAGIPNLLKLAPQELL-PNKKISEMKGTTERSKLDRKRKKK 765
            D K W++KGEV A D  +N   E  ++     +  H        + D+I+ +I++A+ K  +F + V K K   SQ+   V+     + SL ++Y N+  ++    K Q D   ++ + EM+ LF +LD+L H +++P   + E  VL     QS Q    EE G A       LAP+E+  P  ++  +KG TE +  DR+R +K
Sbjct:   36 DDKPWYLKGEVVAQDRAENTVLEEYLD-----VQRHGKFKPPPADEDKILEFIKKAI-KEQSFDSPVFKRKEKTSQSTSQVVPITAQQKSLVEDYENLFAKKNLLEKEQEDPVKNSIQAEMRELFEKLDSLSHLHFVPYKHSPEATVL-----QSKQAMVMEEAGPAAASTADLLAPEEVFAPRGEV--LKGETELTSTDRRRHRK 228
[Close]
BLAST of SMED30007172 vs. TrEMBL
Match: A0A3R7FIX3 (U3 small nucleolar ribonucleoprotein MPP10 OS=Clonorchis sinensis OX=79923 GN=Mphosph10 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.926e-11
Identity = 62/206 (30.10%), Postives = 103/206 (50.00%), Query Frame = 1 
Query:  166 DGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDF-DATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQ----EEIGVAGIPNLLKLAPQELL-PNKKISEMKGTTERSKLDRKRKKK 765
            D K W++KGEV A D  +N   E  ++     +  H        + D+I+ +I++A+ K  +F + V K K   SQ+    +     + SL ++Y N+  ++    K Q D   ++ + EM+ LF +LD+L H +++P   + E  VL     QS Q    EE G A       LAP+E+  P  ++  +KG TE +  DR+R +K
Sbjct:  146 DDKPWYLKGEVVAQDRAENTVLEEYLD-----VQRHGKFKPPPADEDKILEFIKKAI-KEQSFDSPVFKCKEKTSQSTSQAVPITAQQKSLVEDYENLFAKKNLLEKEQEDPVKNSIQAEMRELFEKLDSLSHLHFVPYKHSPEATVL-----QSKQAMVMEEAGPAAASTADLLAPEEVFAPRGEV--LKGATELTSTDRRRHRK 338
[Close]
BLAST of SMED30007172 vs. Ensembl Medaka
Match: mphosph10 (M-phase phosphoprotein 10 [Source:NCBI gene;Acc:101169524])

HSP 1 Score: 51.6026 bits (122), Expect = 6.573e-7
Identity = 55/206 (26.70%), Postives = 96/206 (46.60%), Query Frame = 1 
Query:  172 KKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVN---FANHVRKVKPTES---QTNEIVISGEKSKLSLADEYVN--IMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKK 765
            K W + GEV A    +N    + + E VE         +     +E    +E+ +++ +    F + VRK KP E        + +  EKSK SLA+ Y    I + +++  K +       +K M +LF++LD L + ++ P     E+KV+  N      EE+      +   LAP+E+    K  ++ G  ER+K D+KR+++
Sbjct:  374 KPWQLLGEVTAQTRPEN----SMLEEDVEFEQASRAAPAI---TEETTLQLEDIIKQRIKDQAFDDVVRKEKPKEEVFEYKKRLTLDHEKSKQSLAEIYEQEYIKQTQKNTEKEENPAHVEIQKLMDTLFLKLDALSNFHFTPKPPVPEVKVVS-NLPSITMEEVAPVSTSDATLLAPEEIKDKNKAGDILGDIERTKTDKKRERR 571
[Close]
BLAST of SMED30007172 vs. Planmine SMEST
Match: SMESG000020303.1 (SMESG000020303.1)

HSP 1 Score: 463.381 bits (1191), Expect = 5.837e-167
Identity = 228/230 (99.13%), Postives = 229/230 (99.57%), Query Frame = 1 
Query:  118 SN*ARNQLKTFNKLKKDGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ 807
            S+ ARNQLKTFNKLKKDGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ
Sbjct:    2 SSKARNQLKTFNKLKKDGKKWFMKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ 231
[Close]
BLAST of SMED30007172 vs. Planmine SMEST
Match: SMESG000020303.1 (SMESG000020303.1)

HSP 1 Score: 422.55 bits (1085), Expect = 3.661e-151
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 1 
Query:  184 MKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ 807
            MKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ
Sbjct:    1 MKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEAVEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFLKLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQEEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHLESKKAKEQ 208
The following BLAST results are available for this feature:
BLAST of SMED30007172 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MPHOSPH102.631e-726.70M-phase phosphoprotein 10 [Source:HGNC Symbol;Acc:... [more]
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BLAST of SMED30007172 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG130976.214e-826.47gene:FBgn0032051 transcript:FBtr0079697[more]
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BLAST of SMED30007172 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mphosph106.232e-826.92M-phase phosphoprotein 10 (U3 small nucleolar ribo... [more]
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BLAST of SMED30007172 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q810V0|MPP10_MOUSE4.359e-726.92U3 small nucleolar ribonucleoprotein protein MPP10... [more]
sp|O00566|MPP10_HUMAN1.264e-626.70U3 small nucleolar ribonucleoprotein protein MPP10... [more]
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BLAST of SMED30007172 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2KSR17.114e-1230.10U3 small nucleolar RNA-associated protein MPP10 (F... [more]
A0A1S8WRH11.007e-1130.29Mpp10 protein (Fragment) OS=Opisthorchis viverrini... [more]
A0A4S2LGT41.096e-1130.58Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2LN981.373e-1130.58Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A3R7FIX33.926e-1130.10U3 small nucleolar ribonucleoprotein MPP10 OS=Clon... [more]
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BLAST of SMED30007172 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007172 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mphosph106.573e-726.70M-phase phosphoprotein 10 [Source:NCBI gene;Acc:10... [more]
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BLAST of SMED30007172 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000020303.15.837e-16799.13SMESG000020303.1[more]
SMESG000020303.13.661e-151100.00SMESG000020303.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007172 ID=SMED30007172|Name=SMED30007172|organism=Schmidtea mediterranea sexual|type=transcript|length=947bp
GTTCAATTTCAAAGGAATGATAAAATTAAAAAGGTTTGTTTAAATATTCA
GAATTTTAAAACCTTTACGATGTCTTCAAAAGTATGTTTGACATAGCTAA
TAAAAGCAAATAACCATTCTAATTAGGCTCGTAACCAATTAAAAACTTTT
AATAAATTAAAAAAGGATGGTAAAAAATGGTTTATGAAAGGAGAGGTATT
TGCGAGTGATACTATAGACAATGAAACTGGCGAAGCTCCTATTAATGAAA
CTGTTGAAATGATGAATCATCATGTTGGGCATACTTCATTTAAAGTTAAT
TACGACGAAATAGTTGCATATATTGAAGAAGCAGTAGAAAAAAATGTCAA
TTTTGCTAATCATGTTCGTAAAGTAAAACCAACTGAATCTCAGACAAATG
AAATAGTAATTTCCGGTGAAAAATCTAAGTTATCTCTAGCTGATGAGTAT
GTAAACATTATGAAAAGAGAAGAAGATTTTCTCAAATTGCAAGGTGATGA
TTTTGACGCGACGAGAAAAGAAATGAAGAGTTTATTTATTGAACTCGATA
CTCTGTTCCATAATAATTACATTCCTATAAATTCTGCAATTGAATTGAAA
GTACTGGGCAAAAATGCCGATCAATCTGGACAGGAGGAAATCGGTGTTGC
CGGCATTCCTAACTTATTGAAACTGGCTCCACAAGAGTTACTACCGAACA
AAAAAATCAGCGAAATGAAAGGCACGACAGAACGCAGCAAATTAGATAGA
AAGAGAAAGAAGAAAATCAATGGTCAGCATCTCGAAAGTAAAAAGGCCAA
AGAACAATAAATTATTTTGACTGCTTTGATTTGTTATTATTTTTGTTAAT
ATTATACAAATAAATAATTCATAAATAAAAAGAAGCGATAAAGCTATCAA
TGGATCCAAATCAAATTATTTTTTCTTTACTTTCATTTGATTGCTCG
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protein sequence of SMED30007172-orf-1

>SMED30007172-orf-1 ID=SMED30007172-orf-1|Name=SMED30007172-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp
MKGEVFASDTIDNETGEAPINETVEMMNHHVGHTSFKVNYDEIVAYIEEA
VEKNVNFANHVRKVKPTESQTNEIVISGEKSKLSLADEYVNIMKREEDFL
KLQGDDFDATRKEMKSLFIELDTLFHNNYIPINSAIELKVLGKNADQSGQ
EEIGVAGIPNLLKLAPQELLPNKKISEMKGTTERSKLDRKRKKKINGQHL
ESKKAKEQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR012173Mpp10
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005732small nucleolar ribonucleoprotein complex
GO:0034457Mpp10 complex
GO:0005730nucleolus
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: biological process
TermDefinition
GO:0006364rRNA processing
GO:0010923negative regulation of phosphatase activity
GO:0042254ribosome biogenesis
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012173U3 small nucleolar ribonucleoprotein complex, subunit Mpp10PFAMPF04006Mpp10coord: 54..205
e-value: 1.1E-14
score: 53.9
IPR012173U3 small nucleolar ribonucleoprotein complex, subunit Mpp10PANTHERPTHR17039U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10coord: 55..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..208