SMED30007161

Overview
NameSMED30007161
Smed IDSMED30007161
Length (bp)1982
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30007161 (SMED30007161) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30007161 (SMED30007161) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30007161 (SMED30007161) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30007161 (SMED30007161) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007161

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30007161SMESG000070072.1 SmedASXL_070308SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30007161SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SmedASXL_017630SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30007161SMESG000070072.1 SMESG000013579.1 SmedASXL_017634SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30007161SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007161SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30007161SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007161SMESG000070072.1 dd_Smed_v4_1789_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007161SMESG000070072.1 Contig47911newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30007161SMESG000070072.1 Contig47911uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30007161SMESG000070072.1 dd_Smed_v6_1789_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30007161SMESG000070072.1 dd_Smed_v6_1789_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007161 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000067:13302..16181 +9062
v31.017317:12062..14854 +8123
Homology
[Close]
BLAST of SMED30007161 vs. Planmine SMEST
Match: SMESG000070072.1 (SMESG000070072.1)

HSP 1 Score: 709.909 bits (1831), Expect = 0.000e+0
Identity = 341/345 (98.84%), Postives = 343/345 (99.42%), Query Frame = 3 
Query:  114 MLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNREAHNKYLEIFYFSASNALERKKYLKNLTLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1148
            ML+LTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNR AHNKYLEIFYFSASNALERKKYLKNL+LAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIP KGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT
Sbjct:    1 MLILTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNRAAHNKYLEIFYFSASNALERKKYLKNLSLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 345

HSP 2 Score: 327.405 bits (838), Expect = 2.599e-104
Identity = 169/175 (96.57%), Postives = 170/175 (97.14%), Query Frame = 2 
Query: 1451 NPMEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILNQMEAMRKEHQEIRKAFLYFQWNFNTTTYNIQKTQTFTSAFPLSVRFQTEFVEKDHKLFKCGLIVRNDNPQMSVNLIVTYECWISAKSSPLKLIFNVKVKPLXXXXXXXXXXIFPSSKIFGQNLYQTKLSVGCHFIKFQ 1975
            NPMEICGEKCLNSRIFEIEE VKENVRLNEDIKLILNQMEAMRKEHQEIRKAFLYFQWNFNTTTY IQ TQTFTSAFPLSVRFQTEFVEKDHKLFKCGLIVRNDNPQMSVNL VTYECWISAKSSPLKLIFNVKVKPLEENKE+KNFFIFPSSKIFGQNL QTKLSVGCHFIKFQ
Sbjct:  347 NPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILNQMEAMRKEHQEIRKAFLYFQWNFNTTTYEIQTTQTFTSAFPLSVRFQTEFVEKDHKLFKCGLIVRNDNPQMSVNLTVTYECWISAKSSPLKLIFNVKVKPLEENKEKKNFFIFPSSKIFGQNLSQTKLSVGCHFIKFQ 521
[Close]
BLAST of SMED30007161 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 505.753 bits (1301), Expect = 3.377e-176
Identity = 240/349 (68.77%), Postives = 283/349 (81.09%), Query Frame = 3 
Query:  108 IQMLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNREAHNKYLEIFYFSASNALERKKYLKNLTLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQP-IKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1151
            ++M +LT+  F L L+ A+++I LFV+SVSKFPH IG+AFSLDIQSR+ENGKLSSDPSDTM IR+VDLNFTEIERA+LVYG L FMDDEFKG LMD+K+WEKN   T ++ISIIF REAHNKYLEIFYFSA N   R+KYLKNL+LAKRLFHELIDLFIPSTL L++LPIKVDR++IIINDMVMASC+AFGGIPQEKPRFEDHA LNVTEIIYN+TANEI MNLR SL DHLTNFSC AGNVTETF I IHHHP+ ++I+P IK   Q+N  I+CY  DGYP P +++QLI+GP NA+ +Y   + G +IYI SN+ T S W F+C M S +        K I+F I S
Sbjct:    1 MKMQMLTVLLFILHLLFAEAEIALFVRSVSKFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKDIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKCLMISTIAGVAMTKEKNINFRIAS 349
[Close]
BLAST of SMED30007161 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 504.982 bits (1299), Expect = 8.280e-176
Identity = 240/347 (69.16%), Postives = 281/347 (80.98%), Query Frame = 3 
Query:  114 MLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNREAHNKYLEIFYFSASNALERKKYLKNLTLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQP-IKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1151
            M +LT+  F L L+ A+++I LFV+SVSKFPH IG+AFSLDIQSR+ENGKLSSDPSDTM IR+VDLNFTEIERA+LVYG L FMDDEFKG LMD+K+WEKN   T ++ISIIF REAHNKYLEIFYFSA N   R+KYLKNL+LAKRLFHELIDLFIPSTL L++LPIKVDR++IIINDMVMASC+AFGGIPQEKPRFEDHA LNVTEIIYN+TANEI MNLR SL DHLTNFSC AGNVTETF I IHHHP+ ++I+P IK   Q+N  I+CY  DGYP P +++QLI+GP NA+ +Y   + G +IYI SN+ T S W F+C M S +        K I+F I S
Sbjct:    1 MQMLTVLLFILHLLFAEAEIALFVRSVSKFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKDIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKCLMISTIAGVAMTKEKNINFRIAS 347
[Close]
BLAST of SMED30007161 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 502.671 bits (1293), Expect = 4.493e-175
Identity = 239/345 (69.28%), Postives = 280/345 (81.16%), Query Frame = 3 
Query:  120 VLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNREAHNKYLEIFYFSASNALERKKYLKNLTLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQP-IKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1151
            +LT+  F L L+ A+++I LFV+SVSKFPH IG+AFSLDIQSR+ENGKLSSDPSDTM IR+VDLNFTEIERA+LVYG L FMDDEFKG LMD+K+WEKN   T ++ISIIF REAHNKYLEIFYFSA N   R+KYLKNL+LAKRLFHELIDLFIPSTL L++LPIKVDR++IIINDMVMASC+AFGGIPQEKPRFEDHA LNVTEIIYN+TANEI MNLR SL DHLTNFSC AGNVTETF I IHHHP+ ++I+P IK   Q+N  I+CY  DGYP P +++QLI+GP NA+ +Y   + G +IYI SN+ T S W F+C M S +        K I+F I S
Sbjct:    1 MLTVLLFILHLLFAEAEIALFVRSVSKFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKDIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKCLMISTIAGVAMTKEKNINFRIAS 345
[Close]
BLAST of SMED30007161 vs. Planmine SMEST
Match: SMESG000014563.1 (SMESG000014563.1)

HSP 1 Score: 504.982 bits (1299), Expect = 2.401e-173
Identity = 241/347 (69.45%), Postives = 280/347 (80.69%), Query Frame = 3 
Query:  114 MLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNREAHNKYLEIFYFSASNALERKKYLKNLTLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQP-IKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1151
            M +LT+  F L L+ A+++I LFV+SVS FPH IG+AFSLDIQSR+ENGKLSSDPSDTM IRIVDLNFTEIERA+LVYG L FMDDEFKG LMD+K+WEKN   T ++ISIIF REAHNKYLEI YFSA N   R+KYLKNL+LAKRLFHELIDLFIPSTL L+VLPIKVDR+EIIIND+VMASC+AFGGIPQEKPRFEDHA LNVTEIIYN+TANEI MNLR SL DHLTNFSC AGNVTETF IRIHHHP+ ++I+P IK   Q+N  I+CY  DGYP P +++QLI+ P NA+ +Y   + G +IYI S++ TGS W F+C MKS +        K I F I S
Sbjct:    1 MQMLTVLLFILHLLFAEAEIALFVRSVSNFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDIVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKSPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNIPFRIAS 347

HSP 2 Score: 178.718 bits (452), Expect = 1.325e-48
Identity = 91/135 (67.41%), Postives = 105/135 (77.78%), Query Frame = 3 
Query:  750 KTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQPI-KKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1151
            K+  EI+ ++++ L +      CV  N T +F  RI +HPTALYIQPI KKRF+SNSTIKC+T DGYPDPEINWQLIEGP NA+ FYNIP KGDQIYINSNS  GSKWT +CTMKS LVNT K LNKTISF+I S
Sbjct:  399 KSEWEIKCSVKSCLEN-----VCVILNRTISF--RIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPSNADRFYNIPGKGDQIYINSNSPVGSKWTLKCTMKSILVNTKKSLNKTISFSIAS 526

HSP 3 Score: 73.1738 bits (178), Expect = 2.407e-13
Identity = 58/191 (30.37%), Postives = 84/191 (43.98%), Query Frame = 3 
Query:  603 SVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASL-TDHLTNFSC--------VAGNVTETFTIRIHHHPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1148
            S + IK D ++I+  +  +  C A  G PQ    F+     +     Y    N   + +R+S  T     F C        VA    +    RI  HPT++ I P+               DG  +P      I G  +A+L+YNI R G QI++  N  T S+W  +C++KS L N   ILN+TISF I 
Sbjct:  253 SKIHIKPDIKDILQANRAI-QCYAQDGYPQPSLHFQLIKSPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNIPFRIASHPTSILIMPVP--------------DGIMEPNSTIAFIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIA 428
The following BLAST results are available for this feature:
BLAST of SMED30007161 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007161 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070072.12.599e-10498.84SMESG000070072.1[more]
SMESG000014554.13.377e-17668.77SMESG000014554.1[more]
SMESG000014554.18.280e-17669.16SMESG000014554.1[more]
SMESG000014554.14.493e-17569.28SMESG000014554.1[more]
SMESG000014563.12.401e-17369.45SMESG000014563.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007161 ID=SMED30007161|Name=SMED30007161|organism=Schmidtea mediterranea sexual|type=transcript|length=1982bp
CGGAAGTTGAAAATTGAGGTTGATTAACCCTTGTCCTTTGATCAGTTTAT
GTTGGCTTTCAATCATTATAAATTGCGACCTCCTTCATAAACACATTCTA
ATTCAATATTCAAATGCTAGTTCTAACACTTTATTGTTTCTTTTTACCTT
TGATATTGGCGCAATCTCAAATTCCATTGTTTGTCAAATCGGTCTCGAAA
TTTCCACATTTAATTGGTGACGCCTTTTCACTGGATATCCAATCACGAAC
CGAGAATGGCAAGTTGAGTTCTGATCCGTCAGATACGATGGCCATACGTA
TTGTGGATCTCAATTTTACTGAGATAGAAAGAGCGTTTCTTGTTTATGGG
GTTCTAAATTTTATGGATGACGAGTTCAAAGGGAAATTAATGGACGTCAA
AGTATGGGAAAAGAATCCTACGTTAACAGCAATGAATATATCCATAATAT
TCAATCGAGAGGCCCATAACAAATATCTAGAAATATTTTACTTTTCAGCT
TCCAATGCACTTGAACGAAAAAAATATTTGAAGAATCTCACCCTTGCCAA
GCGGTTATTTCATGAACTGATTGACCTGTTTATCCCATCCACATTGACAC
TCTCGGTGCTCCCTATCAAGGTTGACAGAAGAGAAATAATAATAAATGAT
ATGGTAATGGCATCCTGCAAAGCATTCGGTGGGATACCACAAGAAAAACC
GAGATTCGAGGATCACGCTCACTTGAATGTGACAGAGATAATATATAATA
AAACGGCCAATGAGATCAGAATGAATTTGCGTGCATCTCTCACTGACCAC
CTCACTAATTTCAGCTGCGTCGCAGGAAATGTCACGGAAACGTTCACAAT
CCGTATTCATCATCACCCGACCGCGTTATATATTCAGCCAATCAAAAAAA
GATTTCAATCAAACTCAACAATAAAATGCTATACAAATGATGGATACCCA
GATCCAGAAATAAATTGGCAATTAATTGAAGGCCCCCCAAATGCTAACTT
GTTTTACAATATTCCTCGAAAGGGTGACCAAATCTACATCAATAGCAACA
GCCAAACCGGTTCAAAATGGACGTTCAGATGCACAATGAAAAGCTTTCTC
GTGAATACCAATAAAATTTTGAATAAAACTATTTCATTTGCTATTACTAG
TAAGCTTTTGATTGGATACTATTTCTTTGTTTTGATTGGGTAATATTTGT
TGGTTTTGATTGGATATGATCTGGCGGTGGATATGATCATGATTAATATT
GATACCCTTGTCATAATTATTTTAGGGAGCCCTCAATAATGAATTATCCA
GTCAACATGGTAAAATCCAAACTGCAAATATTATATATTCTTTTCGTTAT
TTCACTGTTACTTCTCTTTTTTACTGATTATATTTTGAATTGATACTTTG
CAAATTTCTATTCTGTTTGATTTGTTTTAGTATTAATCGTTATTTTAGGG
AATCCGATGGAAATTTGTGGGGAGAAATGTTTAAATTCACGGATTTTTGA
AATTGAAGAAGCGGTAAAGGAGAACGTGAGGCTAAATGAGGATATCAAGT
TGATATTGAATCAAATGGAAGCCATGAGAAAGGAACACCAAGAAATAAGA
AAGGCGTTTCTATATTTTCAATGGAATTTTAATACCACAACTTATAATAT
ACAAAAAACCCAAACTTTTACTTCAGCGTTCCCATTGTCTGTTCGATTCC
AAACGGAATTCGTGGAAAAGGACCACAAACTATTCAAATGCGGATTGATA
GTTAGGAATGACAATCCACAAATGTCTGTAAACTTGATAGTGACATACGA
ATGTTGGATATCTGCAAAATCGTCGCCCCTCAAATTGATATTTAATGTTA
AAGTCAAACCTCTAGAAGAAAATAAAGAAGAAAAAAATTTCTTCATATTT
CCTTCTAGTAAGATATTTGGCCAAAACCTTTATCAAACGAAACTCTCTGT
TGGATGTCATTTCATTAAATTTCAATAATAAA
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protein sequence of SMED30007161-orf-1

>SMED30007161-orf-1 ID=SMED30007161-orf-1|Name=SMED30007161-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
MEICGEKCLNSRIFEIEEAVKENVRLNEDIKLILNQMEAMRKEHQEIRKA
FLYFQWNFNTTTYNIQKTQTFTSAFPLSVRFQTEFVEKDHKLFKCGLIVR
NDNPQMSVNLIVTYECWISAKSSPLKLIFNVKVKPLEENKEEKNFFIFPS
SKIFGQNLYQTKLSVGCHFIKFQ*
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protein sequence of SMED30007161-orf-2

>SMED30007161-orf-2 ID=SMED30007161-orf-2|Name=SMED30007161-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=360bp
MLVLTLYCFFLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGK
LSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEK
NPTLTAMNISIIFNREAHNKYLEIFYFSASNALERKKYLKNLTLAKRLFH
ELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFED
HAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHH
HPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNI
PRKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITSKLLI
GYYFFVLIG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 252..341
score: 7.322
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.887