SMED30007155

Overview
NameSMED30007155
Smed IDSMED30007155
Length (bp)430
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007155

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30007155SMESG000021277.1 SmedASXL_018902SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30007155SMESG000021277.1 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007155SMESG000021277.1 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30007155SMESG000021277.1 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30007155SMESG000021277.1 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30007155SMESG000021277.1 dd_Smed_v6_16056_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30007155SMESG000021277.1 OX_Smed_1.0.10682ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30007155 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.008280:120..2017 -2113
Homology
BLAST of SMED30007155 vs. Planmine SMEST
Match: SMESG000021277.1 (SMESG000021277.1)

HSP 1 Score: 184.882 bits (468), Expect = 2.613e-63
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 3
Query:   60 ENMVDKGLRKVQISQFETVHSAEKSVLMKCELKFQDSYKRSSLVMVETIKMPSIPILKMDKEYITDKSEVTISCQTYGGNPTPSLTLYKDIRRSK 344
            ENMVDKGLRKVQISQFETVHSAEKSVLMKCELKFQDSYKRSSLVMVETIKMPSIPILKMDKEYITDKSEVTISCQTYGGNPTPSLTLYK  ++ K
Sbjct:  485 ENMVDKGLRKVQISQFETVHSAEKSVLMKCELKFQDSYKRSSLVMVETIKMPSIPILKMDKEYITDKSEVTISCQTYGGNPTPSLTLYKGHQKIK 579          

HSP 2 Score: 74.3294 bits (181), Expect = 2.613e-63
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 2
Query:  329 HQKIKTEFGKSDPNRITLTHKFIARKSMNLDVYR 430
            HQKIKTEFGKSDPNRITLTHKFIARKSMNLDVYR
Sbjct:  575 HQKIKTEFGKSDPNRITLTHKFIARKSMNLDVYR 608          
The following BLAST results are available for this feature:
BLAST of SMED30007155 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30007155 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000021277.12.613e-6394.74SMESG000021277.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007155 ID=SMED30007155|Name=SMED30007155|organism=Schmidtea mediterranea sexual|type=transcript|length=430bp
GTTTTAATTCATATTATTCTGATTCTACCTGATAAAGCTTAGAGGAAGAT
TTCCTTTAGGAAAACATGGTAGATAAAGGATTGCGAAAAGTACAAATATC
CCAATTTGAAACAGTTCATTCCGCCGAAAAATCCGTCTTGATGAAATGCG
AGTTAAAATTCCAAGATAGCTACAAACGCTCCAGTTTGGTTATGGTGGAG
ACAATAAAAATGCCGTCTATTCCTATATTGAAGATGGATAAAGAATATAT
AACGGATAAATCAGAAGTAACAATTAGTTGCCAAACTTATGGGGGTAACC
CAACACCATCACTTACTTTATATAAAGACATCAGAAGATCAAAACAGAGT
TCGGAAAAAGCGATCCAAATAGAATAACGTTAACTCACAAATTTATTGCC
AGAAAATCGATGAATTTGGACGTGTACCGG
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protein sequence of SMED30007155-orf-1

>SMED30007155-orf-1 ID=SMED30007155-orf-1|Name=SMED30007155-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=104bp
MVDKGLRKVQISQFETVHSAEKSVLMKCELKFQDSYKRSSLVMVETIKMP
SIPILKMDKEYITDKSEVTISCQTYGGNPTPSLTLYKDIRRSKQSSEKAI
QIE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000418head
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 50..103
e-value: 4.0E-5
score: 25.7
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 50..94