Sperm surface protein Sp17

Overview


Name	Sperm surface protein Sp17
Smed ID	SMED30007142
Length (bp)	1792

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Sperm surface protein Sp17 (SMED30007142) t-SNE clustered cells

Violin plots show distribution of expression levels for Sperm surface protein Sp17 (SMED30007142) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sperm surface protein Sp17 (SMED30007142) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sperm surface protein Sp17 (SMED30007142) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30007142

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 48

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30007142	SMESG000046919.1	Contig367	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30007142	SMESG000046919.1	Contig367	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30007142	SMESG000062750.1	Contig367	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30007142	SMESG000062750.1	Contig367	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
head region	SMED30007142	SMESG000046919.1	SmedASXL_015865	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30007142	SMESG000046919.1	SmedASXL_015863	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30007142		SmedASXL_074411	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig26910	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig26910	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig3808	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig3808	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig10753	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig10753	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig36377	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig36377	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig31036	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig31036	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000004166.1	Contig3808	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000004166.1	Contig3808	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
pharynx	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig9154	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30007142	SMESG000046919.1	Contig9154	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30007142	SMESG000046919.1	dd_Smed_v6_2279_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30007142	SMESG000046919.1	dd_Smed_v6_4783_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
reproductive organ	SMED30007142	SMESG000046919.1	Contig26910	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30007142	SMESG000046919.1	Contig26910	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
epidermis	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30007142	SMESG000046919.1	dd_Smed_v4_2279_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
epidermis	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30007142	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Sperm surface protein Sp17 vs. Ensembl Human
Match: SPA17 (sperm autoantigenic protein 17 [Source:HGNC Symbol;Acc:HGNC:11210])

HSP 1 Score: 97.4413 bits (241), Expect = 3.252e-23
Identity = 63/136 (46.32%), Postives = 88/136 (64.71%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 479
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAK 135

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Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 479
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAK 135

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BLAST of Sperm surface protein Sp17 vs. Ensembl Human
Match: CABYR (calcium binding tyrosine phosphorylation regulated [Source:HGNC Symbol;Acc:HGNC:15569])

HSP 1 Score: 51.6026 bits (122), Expect = 5.920e-8
Identity = 25/51 (49.02%), Postives = 31/51 (60.78%), Query Frame = 3

Query:   96 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYD 248
            S  RL VP G + +LEG++R VL+  P NI  FAA YF+EL   R  T  D
Sbjct:    4 SKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMD 54

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Query:   96 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYD 248
            S  RL VP G + +LEG++R VL+  P NI  FAA YF+EL   R  T  D
Sbjct:    4 SKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMD 54

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Query:   96 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYD 248
            S  RL VP G + +LEG++R VL+  P NI  FAA YF+EL   R  T  D
Sbjct:    4 SKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMD 54

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BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Match: igl (gene:FBgn0013467 transcript:FBtr0087362)

HSP 1 Score: 45.8246 bits (107), Expect = 9.649e-6
Identity = 19/28 (67.86%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  759 IDIDLTDPDVEKAAVKIQSAFKGFKTRK 842
            +DIDLTDP++ KAA KIQ++F+G KTRK
Sbjct:   90 LDIDLTDPELNKAATKIQASFRGHKTRK 117

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BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Match: igl (gene:FBgn0013467 transcript:FBtr0332267)

HSP 1 Score: 45.8246 bits (107), Expect = 9.649e-6
Identity = 19/28 (67.86%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  759 IDIDLTDPDVEKAAVKIQSAFKGFKTRK 842
            +DIDLTDP++ KAA KIQ++F+G KTRK
Sbjct:   90 LDIDLTDPELNKAATKIQASFRGHKTRK 117

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BLAST of Sperm surface protein Sp17 vs. Ensembl Zebrafish
Match: spa17 (sperm autoantigenic protein 17 [Source:ZFIN;Acc:ZDB-GENE-061027-337])

HSP 1 Score: 106.301 bits (264), Expect = 1.641e-23
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 290
            MAVP+SNT LR+P+GF N+LEGL REVLR QPE+I +FAA YF ELL+ R+E+G  D A  GAKLEDRFY
Sbjct:    1 MAVPFSNTHLRIPRGFGNLLEGLTREVLREQPEDIATFAAVYFTELLKAREESGL-DPAEWGAKLEDRFY 69

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BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
Match: mettl4 (methyltransferase like 4 [Source:Xenbase;Acc:XB-GENE-992320])

HSP 1 Score: 95.9005 bits (237), Expect = 2.415e-20
Identity = 59/148 (39.86%), Postives = 85/148 (57.43%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEE--------QQSAAVKIQSEYRRFAAKK-------EVDTKKEEEAAIVIQASFRGHFERQNQK 479
            M++P+SNT  R+P+GF N+LEGL REVLR QP++I  F A YF +LL+ RQ+T +D  A  GA LEDRFYNN+ F++        E        ++S ++   S+    +          E D  ++ +AA  IQA+FRG+  R   K
Sbjct:    1 MSIPFSNTHYRIPRGFANLLEGLTREVLRHQPKDIPLFGAKYFSQLLQQRQDTDFDP-AQWGAALEDRFYNNYDFQHTELKKYTSEPSELKKSKRKSFSMLDNSDTYPLSPSTSLEQKEIETDDMRKMQAATTIQAAFRGYNVRHKVK 147

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Spa17 (sperm autoantigenic protein 17 [Source:MGI Symbol;Acc:MGI:1333778])

HSP 1 Score: 97.4413 bits (241), Expect = 2.670e-23
Identity = 66/151 (43.71%), Postives = 96/151 (63.58%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQS-AAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTN 530
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK+EDRFYNNH+FK        E++ + ++ + ++    F    E + ++EE AA+ IQ+ FRGH  R        EE+ ++ SD   N
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDP-AEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVAR--------EEVKKMKSDKNEN 142

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Spa17 (sperm autoantigenic protein 17 [Source:MGI Symbol;Acc:MGI:1333778])

HSP 1 Score: 93.5893 bits (231), Expect = 3.069e-22
Identity = 61/131 (46.56%), Postives = 89/131 (67.94%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQS-AAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQ 470
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK+EDRFYNNH+FK        E++ + ++ + ++    F    E + ++EE AA+ IQ+ FRGH  R+
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDP-AEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVARE 130

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Cabyr (calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) [Source:MGI Symbol;Acc:MGI:1918382])

HSP 1 Score: 53.9138 bits (128), Expect = 6.361e-8
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:   96 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDI 254
            S  RL VP G + +LEG++R +L+  P NI  FAA YF+EL+  R+     DI
Sbjct:    4 SKPRLVVPYGLKTLLEGVSRAILKTNPTNITQFAAVYFKELIVFREGNSSLDI 56

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Cabyr (calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) [Source:MGI Symbol;Acc:MGI:1918382])

HSP 1 Score: 53.9138 bits (128), Expect = 1.123e-7
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:   96 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDI 254
            S  RL VP G + +LEG++R +L+  P NI  FAA YF+EL+  R+     DI
Sbjct:    4 SKPRLVVPYGLKTLLEGVSRAILKTNPTNITQFAAVYFKELIVFREGNSSLDI 56

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Cabyr (calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) [Source:MGI Symbol;Acc:MGI:1918382])

HSP 1 Score: 54.6842 bits (130), Expect = 2.359e-7
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 3

Query:   96 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDI 254
            S  RL VP G + +LEG++R +L+  P NI  FAA YF+EL+  R+     DI
Sbjct:    4 SKPRLVVPYGLKTLLEGVSRAILKTNPTNITQFAAVYFKELIVFREGNSSLDI 56

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|P36425|SP17_RABIT (Sperm surface protein Sp17 OS=Oryctolagus cuniculus OX=9986 GN=SPA17 PE=1 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 4.200e-28
Identity = 65/133 (48.87%), Postives = 90/133 (67.67%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 479
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK++DRFYNNH+F+   +     E++S +V  + E        E D  KEE AA+ IQA+FRGH  R++ K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTNFDP-AEWGAKVDDRFYNNHAFQEHESEKCEAEEKSQSV-TEEETPVLTIDSEDDKDKEEMAALKIQAAFRGHLAREDVK 131

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|O19021|SP17_MACFA (Sperm surface protein Sp17 OS=Macaca fascicularis OX=9541 GN=SPA17 PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 5.203e-23
Identity = 66/161 (40.99%), Postives = 95/161 (59.01%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQ----------SAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQ 533
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       ++ +Q           A+V I         K+EV       AA+ IQA+FRGH  R        EE+ ++ +D+  N+
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQIPGKEEEASVTILDSSEEDKEKEEV-------AAVKIQAAFRGHVAR--------EEVKKMKTDSLQNE 145

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|Q95230|SP17_PAPHA (Sperm surface protein Sp17 OS=Papio hamadryas OX=9557 GN=SPA17 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 8.740e-23
Identity = 66/154 (42.86%), Postives = 96/154 (62.34%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQ 533
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R        EE+ ++ +D+  N+
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQVSGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHVAR--------EEVKKMKTDSLQNE 145

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|Q15506|SP17_HUMAN (Sperm surface protein Sp17 OS=Homo sapiens OX=9606 GN=SPA17 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.562e-22
Identity = 63/136 (46.32%), Postives = 88/136 (64.71%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 479
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAK 135

[Close]

BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|Q62252|SP17_MOUSE (Sperm surface protein Sp17 OS=Mus musculus OX=10090 GN=Spa17 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.867e-22
Identity = 66/151 (43.71%), Postives = 96/151 (63.58%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQS-AAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTN 530
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK+EDRFYNNH+FK        E++ + ++ + ++    F    E + ++EE AA+ IQ+ FRGH  R        EE+ ++ SD   N
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDP-AEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVAR--------EEVKKMKSDKNEN 142

[Close]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A210PVC9 (Sperm surface protein Sp17 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT22352 PE=4 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 7.012e-58
Identity = 164/382 (42.93%), Postives = 231/382 (60.47%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKEN-DMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTR---KANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIK 1214
            MAVP+SNT+LRVPKGFQN+LEGLAREVLR QPENIY F A YF++LLR+R ETG+D  A+ GA++EDRFYNN +F++P  ++ + +QQ AA+KIQ+EYRR +AKKEVD  +EE+AA  IQA  RGH +RQ        ++D I       +    DI        N+++PE+    E+  +    G    ++ Y          +++  +KE+ + + E  +  +E +DIDL DP+V KAA KIQ+ F+G KTR   K++ED P     ++ EN EP+++          IDL DPEV KA  KIQ+ FKG+++RK +      +  Q L       +K  +       +  DID NDPDV KAA KIQ+ +KG + RK I+
Sbjct:    1 MAVPFSNTKLRVPKGFQNILEGLAREVLRNQPENIYEFGAKYFDQLLRVRDETGHDP-AVHGARMEDRFYNNDAFRSPGVDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREEDAATTIQAGVRGHQDRQ--------KVDEIKEGEARLKSAEVDI--------NLEDPEV----EAAAVKIQAG----FKGY----------KARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAP----KSETENAEPEKID---------IDLNDPEVAKAATKIQAGFKGYKTRKEM------NGKQPLS------DKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIR 322

HSP 2 Score: 97.4413 bits (241), Expect = 3.469e-17
Identity = 112/318 (35.22%), Postives = 158/318 (49.69%), Query Frame = 3

Query:  348 AAVKIQSEYRRFAAKKEVDTKKE--------------------------EEAAIVIQASFRGHFERQNQKPN-SNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPE----PKRLQXXXXXXXX-XXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRK 1205
            AA KIQ+ ++ +  +KE++ K+                            +AA  IQA+F+GH  R+  + N S  E DR  +D   +   +   ++N  +  ++ +PE+           A      Y+          + E KS         E +   EEIIDIDLTDP+VEKAAVKIQS+FKG + RK+   T  A  T+ E  P     P  L    E +   DIDL DPEVEKA +KIQ+TFKG +SR+     +    + +L  ++      DKV     EE IDIDLNDP+V KAA+KIQS +KG ++RK
Sbjct:  253 AATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKA--------ATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSK--TASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRF--GAKKSSPTNNLNNMKPVTPPVDKVPV---EEEIDIDLNDPEVAKAALKIQSTFKGLKSRK 555

HSP 3 Score: 92.4337 bits (228), Expect = 1.295e-15
Identity = 74/155 (47.74%), Postives = 91/155 (58.71%), Query Frame = 3

Query:  759 IDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSR-----KTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKT 1208
            IDIDL DP+VEKAAVKIQ+ FKG K+RK         + N    P P     I  +E IDIDL DPEVEKA +KIQ+TFKG +SR     K +  ++    S H++     +E+ D            IDL DPDVEKAAVKIQ+ +KG R RKT
Sbjct:  702 IDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPP--TVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEID------------IDLTDPDVEKAAVKIQASFKGLRARKT 842

HSP 4 Score: 92.0485 bits (227), Expect = 1.711e-15
Identity = 69/159 (43.40%), Postives = 98/159 (61.64%), Query Frame = 3

Query:  747 SEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXX-XXXXXXXXVEKATIKIQSTFKGFRSRKT--LEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIK 1214
            SEE IDIDL DP+VEKAAVKIQ+ FKG K+RK  +  P+AP+   ++ P    +     +    DIDLTDP+VEKA +KIQ++FKG R+RKT   +E    +  +  E  EE ++               IDLNDP+  +AA+KIQ+ ++G++ RK +K
Sbjct:  754 SEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPL---KQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEID---------------IDLNDPETAQAALKIQAGFRGYKTRKEVK 894

HSP 5 Score: 85.8853 bits (211), Expect = 1.564e-13
Identity = 98/338 (28.99%), Postives = 162/338 (47.93%), Query Frame = 3

Query:  336 EQQSAAVKIQSEY-----RRFAAKKEVDTKKE----------------------------EEAAIVIQASFRGHFERQ-NQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVE--KATIKIQSTFKGFRSRKTLEEM--QTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXX------LNDPDVEKAAVKIQSVYKGFRNRKTIKG 1217
            E + AA+KIQS +     R+F AKK   T  E                            E+AA+ IQA+F+G   R+   K +S   +++IP+   T      +I     +  ++++PE+    ++     A  +    +++        L +  S+    D K  E+    E IDIDLTDPDVEKAAVKIQ++FKG + RK          T +++   PK  ++  E +  +ID+   + E  +A +KIQ+ F+G+++RK ++E     GD+++  +  EE     +       +     +      LNDP+V KAA KIQ+ +KG++ RK +KG
Sbjct:  652 EVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPT-----VNIPSEEEIDIDLNDPEV---EKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSS-NHVDAKSSEV----EEIDIDLTDPDVEKAAVKIQASFKGLRARK----------TKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 966

HSP 6 Score: 83.9593 bits (206), Expect = 6.132e-13
Identity = 100/319 (31.35%), Postives = 150/319 (47.02%), Query Frame = 3

Query:  336 EQQSAAVKIQSEYRRFAAKKEVDTKKEEE----------------------AAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTN----DEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVE--ENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKT 1208
            E ++AAVKIQ+ ++ + A+KEV   KE +                      AA  IQA FRGH  R+  K  S+E       DA  ++  N + E+   +  ++++PE+           A      ++ Y            K  L + D +   L   +   DID  DPDV KAA KIQ+ FKG + RK       AP T+    D    + K + +   +EI         VEKA  KIQ+ +KG ++R+ L+ +Q   +S  + + +  EN+   +++        IDIDL DP+VEKAAVKIQS +KG R RK+
Sbjct:  154 EVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK--SSE-------DAPKSETENAEPEK---IDIDLNDPEVAKA--------ATKIQAGFKGYKTRKEMN----GKQPLSDKDSRTSGLDKCD-TDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPE-VEKAATKIQAGYKGLKTRRELKSIQE-PKSTSIPVRDSMENVPTEEEI--------IDIDLTDPEVEKAAVKIQSSFKGLRARKS 437

HSP 7 Score: 80.4925 bits (197), Expect = 8.609e-12
Identity = 77/160 (48.12%), Postives = 95/160 (59.38%), Query Frame = 3

Query:  762 DIDLTDPDVEKAAVKIQSAFKGFKTRK--ANEDTPIAPVTNDEENPEPKRLQXXXXXXXXX--XXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIKG-PVA 1226
            DIDL DP+VEKAA+KIQS FKG K+RK  A + +P    TN E    P     +      +  IDL DPEVEKA +KIQ+TFKG +SRK       G +     +V +       V     EE IDIDLNDP+VEKAAVKIQ+ +KG ++RK  K  PVA
Sbjct:  645 DIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSP----TNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRK------FGSKKSSPSLVNKIPTPPPTVN-IPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVA 793

HSP 8 Score: 77.7962 bits (190), Expect = 4.545e-11
Identity = 107/344 (31.10%), Postives = 154/344 (44.77%), Query Frame = 3

Query:  336 EQQSAAVKIQSEYRRFAAKKE------------------------VDTKKEEE------------AAIVIQASFRG----HFERQNQKPNSN--------EEIDRIPSDAQTN-QHNNTDIEENTL----VHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRK-ANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRK 1205
            E + AAVKIQS ++   A+K                         V T+++EE            AA+ IQA+F+G     F  +   P +N          +D++P + + +   N+ ++ +  L        +   +      S+  PP +  N                            P      E+ IDIDL DP+V KAAVKIQS FKG K+RK   + +  +P +N E +P       +  +E +DIDL DPEVEKA +KIQSTFKG +SRK         +S    I   +            +E IDIDLNDP+VEKAAVKIQ+ +KG ++RK
Sbjct:  417 EVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPS------------------------PPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPP--PPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKF-----GAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRK 729

[Close]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: K1PZL4 (Sperm surface protein Sp17 OS=Crassostrea gigas OX=29159 GN=CGI_10005609 PE=4 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.488e-45
Identity = 136/387 (35.14%), Postives = 216/387 (55.81%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQ------------NQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRK 1205
            MAVP+SNT+LRVPKGFQN+LEGLARE LR QPENIY F A YF++L+++R++TG+D  A+ GA+LEDRFYNN SF++P+T+  + +QQ AA+KIQ+E+RR  A++EV+  KEE+AA+ IQ+ FRG  +RQ             QK  ++E + +  S       ++ DI        ++D+PE+ +         A      ++ Y    ++  + + K     +     +L + +E+ D          AA KIQ+ F+G KTR+  ++       +                  +  ++   E+E A + IQ+ F+G+++RK ++ +    +SQ  +  EE ++               IDLNDPDVE+AA+KIQ+ +KG++ RK
Sbjct:    1 MAVPFSNTKLRVPKGFQNILEGLARECLRSQPENIYEFGAKYFQQLIQVREQTGHDP-AVHGARLEDRFYNNDSFQSPSTDPGDPQQQEAALKIQTEFRRHHAEQEVEHMKEEDAAVKIQSGFRGFQDRQKVLEMKDPEAYAKQKEEADERLSKGGSS-----KDSVDI--------DLDDPEVAE--------AAVKIQAGFKGY---KARKEVKDKKEKASTDSKSDSQLPSEKEMAD----------AATKIQAGFRGHKTRQDMKEKKEKGKED------------------MLSEINKSELENAVVHIQAGFRGYQARKEMKHLM---DSQPAKQPEEEVD---------------IDLNDPDVEQAALKIQAGFKGYKTRK 316

HSP 2 Score: 92.4337 bits (228), Expect = 6.718e-16
Identity = 100/316 (31.65%), Postives = 157/316 (49.68%), Query Frame = 3

Query:  336 EQQSAAVKIQSEYRRFAAKKEVD-------TKKEEE-------------AAIVIQASFRGHFERQ---NQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIK 1214
            E ++A V IQ+ +R + A+KE+         K+ EE             AA+ IQA F+G+  R+   ++K  S+E I+     A  N+    DI        ++++P         D+  A  + Q      I+  ++  D +KS++ + +  P   Q SEE+IDIDL DP+VEKAA+KIQ+ FKGF  RKA + T        E  PE          E       D E E+A +KIQ+ F+G+++R+ ++E    +E +  E  E +++                 LNDPDVE+AA+KIQ+ +KG++ RK IK
Sbjct:  252 ELENAVVHIQAGFRGYQARKEMKHLMDSQPAKQPEEEVDIDLNDPDVEQAALKIQAGFKGYKTRKEFESKKMESSEVIN-----AGKNEEEEVDI--------DLNDP---------DVANAAVKIQAGFRAHIKAKKS--DTAKSSVTK-ESSPVAEQESEEVIDIDLNDPEVEKAALKIQAGFKGF--RKAKKATTQDQTDKTESKPE----------EETSSKAADKETEEAALKIQAGFRGYKTRQEVKEQMAKEEKKGEEQEEVDID-----------------LNDPDVEQAALKIQAGFKGYQARKEIK 513

[Close]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A1I8IBG4 (RIIa domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 5.836e-42
Identity = 141/413 (34.14%), Postives = 205/413 (49.64%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEE--QQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDM---KPEELQ------------------NSEEIIDIDLTDPDVEKAAVKIQSAFKGF----KTRKANEDTPIAPVTNDEE------NPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIKGP 1220
            M+ P SN+RLRVP GFQ +LEG  REVLR QP +IYSF A YFEE+LR R+ETG +D A L  +L+DR  N   F +     +  +  QQ A+V IQS++RR AA++E +  + + AA  IQA+ RGH ER+N              D+ T   ++ D  E   V  ++++PE+           A      ++ Y         D+ K +  E D+    PE  +                  N+ + +DIDLTDP+V  AA KIQ+ FKG+    + ++  ++    P   D E      +PE                     V  A  KIQ+ FKG+++RK +++ Q  DE Q  E       K+D+       E +DIDL DP+V  AA KIQ+ +KG++ RK +K P
Sbjct:    1 MSAPISNSRLRVPHGFQALLEGFTREVLRNQPSDIYSFGALYFEEMLRRREETGEEDPAQLALRLQDRLRNGAKFGDGRVIGDASDARQQQASVVIQSDFRRRAAQREAEQLRRDRAAGDIQAAVRGHQERRN-------------GDSAT--RDDQDAAEEDEVDIDLNDPEVA--------AAAEKIQAGFKGYKTRKQMKNQDKKKPDEDEVDIDLNDPEGYKTRKQMKQQQQQSSKPSGENATDEVDIDLTDPEVGAAAEKIQAGFKGYKTRKQMKQQKQEQQTKPAEADTEVDIDLTDPE---------------------VGAAAEKIQAGFKGYKTRKQMKQQQ--DEKQQSEA------KADE-------EEVDIDLTDPEVGAAAEKIQAGFKGYKTRKQMKQP 354

[Close]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A1I8H9A3 (RIIa domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.482e-41
Identity = 137/402 (34.08%), Postives = 200/402 (49.75%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEE--QQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKEN------------DMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGF----KTRKANEDTPIAPVTNDEE------NPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIK 1214
            M+ P SN+RLRVP GFQ +LEG  REVLR QP +IYSF A YFEE+LR R+ETG +D A L  +L+DR  N   F +     +  +  QQ A+V IQS++RR AA++E +  + + AA  IQA+ RGH ER+N              D+ T   ++ D  E   V  ++++PE+           A      ++ Y         D+ K +  E                    +N+ + +DIDLTDP+V  AA KIQ+ FKG+    + ++  ++    P   D E      +PE                     V  A  KIQ+ FKG+++RK +++ Q  DE Q  E       K+D+       E +DIDL DP+V  AA KIQ+ +KG++ RK +K
Sbjct:    1 MSAPISNSRLRVPHGFQALLEGFTREVLRNQPSDIYSFGALYFEEMLRRREETGEEDPAQLALRLQDRLRNGAKFGDGRVVGDASDARQQQASVVIQSDFRRRAAQREAEQLRRDRAAGDIQAAVRGHQERRN-------------GDSAT--RDDQDAAEEDEVDIDLNDPEVA--------AAAEKIQAGFKGYKTRKQMKNQDKKKPDEDEGYKTRKQMKQQQQQSSKPSGENATDEVDIDLTDPEVGAAAEKIQAGFKGYKTRKQMKQQKQEQQTKPAEADTEVDIDLTDPE---------------------VGAAAEKIQAGFKGYKTRKQMKQQQ--DEKQQSEA------KADE-------EEVDIDLTDPEVGAAAEKIQAGFKGYKTRKQMK 343

[Close]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A1I8I308 (RIIa domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 3.338e-41
Identity = 140/411 (34.06%), Postives = 204/411 (49.64%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEE--QQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDM---KPEELQ------------------NSEEIIDIDLTDPDVEKAAVKIQSAFKGF----KTRKANEDTPIAPVTNDEE------NPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIK 1214
            M+ P SN+RLRVP GFQ +LEG  REVLR QP +IYSF A YFEE+LR R+ETG +D A L  +L+DR  N   F +     +  +  QQ A+V IQS++RR AA++E +  + + AA  IQA+ RGH ER+N              D+ T   ++ D  E   V  ++++PE+           A      ++ Y         D+ K +  E D+    PE  +                  N+ + +DIDLTDP+V  AA KIQ+ FKG+    + ++  ++    P   D E      +PE                     V  A  KIQ+ FKG+++RK +++ Q  DE Q  E       K+D+       E +DIDL DP+V  AA KIQ+ +KG++ RK +K
Sbjct:    1 MSAPISNSRLRVPHGFQALLEGFTREVLRNQPSDIYSFGALYFEEMLRRREETGEEDPAQLALRLQDRLRNGAKFGDGRVIGDVSDARQQQASVVIQSDFRRRAAQREAEQLRRDRAAGDIQAAVRGHQERRN-------------GDSAT--RDDQDAAEEDEVDIDLNDPEVA--------AAAEKIQAGFKGYKTRKQMKNQDKKKPDEDEVDIDLNDPEGYKTRKQMKQQQQQSSKPSGENATDEVDIDLTDPEVGAAAEKIQAGFKGYKTRKQMKQQKQEQQTKPAEADTEVDIDLTDPE---------------------VGAAAEKIQAGFKGYKTRKQMKQQQ--DEKQQSEA------KADE-------EEVDIDLTDPEVGAAAEKIQAGFKGYKTRKQMK 352

[Close]

BLAST of Sperm surface protein Sp17 vs. Ensembl Cavefish
Match: ENSAMXT00000046795.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:22320681:22325023:1 gene:ENSAMXG00000038900.1 transcript:ENSAMXT00000046795.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.8265 bits (242), Expect = 3.507e-23
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 290
            M+VP+SNT LRVP GF N+LEGLAREVLR QP++I +FAA YF  LL+ R E+G D  A  GA+LEDRFY
Sbjct:    1 MSVPFSNTNLRVPHGFGNILEGLAREVLREQPDDIPTFAAVYFTALLKQRDESGLDP-AEWGARLEDRFY 69

[Close]

BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
Match: EDO46037 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR24])

HSP 1 Score: 102.449 bits (254), Expect = 1.177e-25
Identity = 62/138 (44.93%), Postives = 85/138 (61.59%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKK-----EEEAAIVIQASFRGHFERQNQK 479
            MAV Y+ T+LRVP GFQN+LEGLAREVLR QP++I  FAA YF+  L +R+ETG DD     AK  +        +  + + ++ E Q AA KIQ+ +R    ++++   K     EE AA+ IQ+SFRGH  R+  K
Sbjct:    1 MAVQYAPTKLRVPPGFQNLLEGLAREVLREQPDDIVQFAAQYFKNQLSIREETGKDD-----AKRGENLEKLMKGEEVDIDIDDPEVQKAATKIQASFRGHKTREQIKQDKQQKDEEEAAALKIQSSFRGHKAREEVK 133

HSP 2 Score: 49.6766 bits (117), Expect = 2.773e-7
Identity = 35/100 (35.00%), Postives = 52/100 (52.00%), Query Frame = 3

Query:  732 EELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEM 1031
            E+L   EE+ DID+ DP+V+KAA KIQ++F+G KTR   E         DEE                         E A +KIQS+F+G ++R+ ++E+
Sbjct:   65 EKLMKGEEV-DIDIDDPEVQKAATKIQASFRGHKTR---EQIKQDKQQKDEE-------------------------EAAALKIQSSFRGHKAREEVKEL 135

[Close]

BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
Match: EDO35043 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMM3])

HSP 1 Score: 71.633 bits (174), Expect = 6.507e-15
Identity = 35/62 (56.45%), Postives = 45/62 (72.58%), Query Frame = 3

Query:  105 RLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 290
            +L VP GF+N+LE L  E+LR +P+NI SFAAD+F E L  RQ  GYDD  LLG+++ D FY
Sbjct:    7 KLSVPSGFRNLLESLTVEILRVRPDNIPSFAADFFRERLAARQRNGYDD-CLLGSQI-DGFY 66

[Close]

BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
Match: ENSORLT00000038228.1 (sperm autoantigenic protein 17 [Source:NCBI gene;Acc:105355434])

HSP 1 Score: 83.9593 bits (206), Expect = 1.784e-16
Identity = 39/70 (55.71%), Postives = 49/70 (70.00%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 290
            M+  +    LRVP+G   V+EGLAREVLR QPENI  +AA+YF  LLR R+E+G  D A   AKLEDR++
Sbjct:    1 MSAHFFTAHLRVPRGLGAVIEGLAREVLRDQPENIPEYAANYFNGLLRQREESGV-DPAAWAAKLEDRYH 69

[Close]

BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
Match: ENSORLT00000038630.1 (sperm autoantigenic protein 17 [Source:NCBI gene;Acc:105355434])

HSP 1 Score: 83.9593 bits (206), Expect = 1.800e-16
Identity = 39/70 (55.71%), Postives = 49/70 (70.00%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 290
            M+  +    LRVP+G   V+EGLAREVLR QPENI  +AA+YF  LLR R+E+G  D A   AKLEDR++
Sbjct:    1 MSAHFFTAHLRVPRGLGAVIEGLAREVLRDQPENIPEYAANYFNGLLRQREESGV-DPAAWAAKLEDRYH 69

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 337/426 (79.11%), Postives = 338/426 (79.34%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIKGPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 1358
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKS+LKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENP                                           EEMQTGDESQHLEIVEENLE                                            GPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENP-------------------------------------------EEMQTGDESQHLEIVEENLE--------------------------------------------GPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 339

HSP 2 Score: 59.6918 bits (143), Expect = 5.055e-9
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 3

Query: 1359 THEDNPESGGPTNDTDEAATSRTEPDE 1439
            THEDNPESGGPTNDTDEAATSRTEPDE
Sbjct:  596 THEDNPESGGPTNDTDEAATSRTEPDE 622

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 512.301 bits (1318), Expect = 2.581e-176
Identity = 295/426 (69.25%), Postives = 296/426 (69.48%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIKGPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 1358
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKS+LKENDM                                          DTPIAPVTNDEENPE                                           EMQTGDESQHLEIVEENLE                                            GPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDM------------------------------------------DTPIAPVTNDEENPE-------------------------------------------EMQTGDESQHLEIVEENLE--------------------------------------------GPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 297

HSP 2 Score: 59.3066 bits (142), Expect = 4.969e-9
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 3

Query: 1359 THEDNPESGGPTNDTDEAATSRTEPDE 1439
            THEDNPESGGPTNDTDEAATSRTEPDE
Sbjct:  554 THEDNPESGGPTNDTDEAATSRTEPDE 580

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 509.605 bits (1311), Expect = 6.071e-175
Identity = 301/432 (69.68%), Postives = 307/432 (71.06%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLE------KSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIKGPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 1358
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKS+LKENDM                                          DTPIAPVTNDEENPE                                           EMQTGDESQHLEIVEENLE        D V+        +I L D   EKAAVKIQSVYKG+R RK                ++KVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDM------------------------------------------DTPIAPVTNDEENPE-------------------------------------------EMQTGDESQHLEIVEENLEGPVAGGHGDYVETH-----EEIHLYDETSEKAAVKIQSVYKGYRTRK----------------NKKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 326

HSP 2 Score: 60.077 bits (144), Expect = 3.382e-9
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 3

Query: 1359 THEDNPESGGPTNDTDEAATSRTEPDE 1439
            THEDNPESGGPTNDTDEAATSRTEPDE
Sbjct:  583 THEDNPESGGPTNDTDEAATSRTEPDE 609

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 492.656 bits (1267), Expect = 5.378e-168
Identity = 295/469 (62.90%), Postives = 296/469 (63.11%), Query Frame = 3

Query:   81 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID-------------------------------------------RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIKGPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 1358
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID                                           RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKS+LKENDM                                          DTPIAPVTNDEENPE                                           EMQTGDESQHLEIVEENLE                                            GPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDM------------------------------------------DTPIAPVTNDEENPE-------------------------------------------EMQTGDESQHLEIVEENLE--------------------------------------------GPVAGGHGDYVETHEKVNEDLEFDHENQEVPTDEITNVTDNMINEAEQ 340

HSP 2 Score: 59.6918 bits (143), Expect = 4.406e-9
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 3

Query: 1359 THEDNPESGGPTNDTDEAATSRTEPDE 1439
            THEDNPESGGPTNDTDEAATSRTEPDE
Sbjct:  597 THEDNPESGGPTNDTDEAATSRTEPDE 623

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000038137.1 (SMESG000038137.1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.744e-7
Identity = 80/314 (25.48%), Postives = 148/314 (47.13%), Query Frame = 3

Query:  348 AAVKIQSEYRRFAAKKEVDTKKE------EEAAIVIQASFRG----HFERQNQKPNSN-----EEIDRIPSDAQTNQHNNTDIEENTL--VHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRK--ANEDTPI---APVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKATIKIQSTFKGFRSRKTLEE---MQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKTIK 1214
            AA KIQ+ +R F  +KE++  KE      ++  + I  S +     H E Q++ P  N     +E   I +  Q  Q+ + ++E  T    ++N ++    DK          G         ++ ++  + +SK +      +PE L  SE+  D ++ D    KAA KIQ+ F+G+ TR+   ++ +P    AP   +E+  EPKR+           D  +  +E A + IQ++F+G+++R TL+     +T +E +      +N E +++ K             D ++EKA + IQ+ ++G+R R ++K
Sbjct:  131 AATKIQAAFRGFQTRKEIEKAKENNKPVSKKHTVYIPKSIKPKNTEHHE-QDKNPKINYSKVKKEEKEIQAKPQKTQNKSRNVESKTTNPKNTNPNKTNADDKRNKAATKIQAGFRGYQTRQRLKFNKPEIKQSKISQNPKKSQPESL-TSEKESDYNIKD----KAAAKIQAGFRGYHTRQILKSQVSPKPYKAPEKKNEKKQEPKRV-------NETNDNNNKAMETAAVIIQASFRGYQTRHTLQNKVPRKTNNEPEKQN--GKNQEPTNENK-------------DKEIEKAVITIQAGFRGYRIRNSLK 416

The following BLAST results are available for this feature:

BLAST of Sperm surface protein Sp17 vs. Ensembl Human
BLAST of Sperm surface protein Sp17 vs. Ensembl Celegans
BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
BLAST of Sperm surface protein Sp17 vs. Ensembl Zebrafish
BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
BLAST of Sperm surface protein Sp17 vs. TrEMBL
BLAST of Sperm surface protein Sp17 vs. Ensembl Cavefish
BLAST of Sperm surface protein Sp17 vs. Ensembl Sea Lamprey
BLAST of Sperm surface protein Sp17 vs. Ensembl Yeast
BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
BLAST of Sperm surface protein Sp17 vs. Planmine SMEST

BLAST of Sperm surface protein Sp17 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SPA17	3.252e-23	46.32	sperm autoantigenic protein 17 [Source:HGNC Symbol...	[more]
SPA17	3.252e-23	46.32	sperm autoantigenic protein 17 [Source:HGNC Symbol...	[more]
CABYR	5.920e-8	49.02	calcium binding tyrosine phosphorylation regulated...	[more]
CABYR	5.920e-8	49.02	calcium binding tyrosine phosphorylation regulated...	[more]
CABYR	5.920e-8	49.02	calcium binding tyrosine phosphorylation regulated...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
igl	9.649e-6	67.86	gene:FBgn0013467 transcript:FBtr0087362	[more]
igl	9.649e-6	67.86	gene:FBgn0013467 transcript:FBtr0332267	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
spa17	1.641e-23	68.57	sperm autoantigenic protein 17 [Source:ZFIN;Acc:ZD...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
mettl4	2.415e-20	39.86	methyltransferase like 4 [Source:Xenbase;Acc:XB-GE...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Spa17	2.670e-23	43.71	sperm autoantigenic protein 17 [Source:MGI Symbol;...	[more]
Spa17	3.069e-22	46.56	sperm autoantigenic protein 17 [Source:MGI Symbol;...	[more]
Cabyr	6.361e-8	45.28	calcium-binding tyrosine-(Y)-phosphorylation regul...	[more]
Cabyr	1.123e-7	45.28	calcium-binding tyrosine-(Y)-phosphorylation regul...	[more]
Cabyr	2.359e-7	45.28	calcium-binding tyrosine-(Y)-phosphorylation regul...	[more]

BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P36425\|SP17_RABIT	4.200e-28	48.87	Sperm surface protein Sp17 OS=Oryctolagus cuniculu...	[more]
sp\|O19021\|SP17_MACFA	5.203e-23	40.99	Sperm surface protein Sp17 OS=Macaca fascicularis ...	[more]
sp\|Q95230\|SP17_PAPHA	8.740e-23	42.86	Sperm surface protein Sp17 OS=Papio hamadryas OX=9...	[more]
sp\|Q15506\|SP17_HUMAN	1.562e-22	46.32	Sperm surface protein Sp17 OS=Homo sapiens OX=9606...	[more]
sp\|Q62252\|SP17_MOUSE	1.867e-22	43.71	Sperm surface protein Sp17 OS=Mus musculus OX=1009...	[more]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A210PVC9	7.012e-58	42.93	Sperm surface protein Sp17 OS=Mizuhopecten yessoen...	[more]
K1PZL4	1.488e-45	35.14	Sperm surface protein Sp17 OS=Crassostrea gigas OX...	[more]
A0A1I8IBG4	5.836e-42	34.14	RIIa domain-containing protein OS=Macrostomum lign...	[more]
A0A1I8H9A3	1.482e-41	34.08	RIIa domain-containing protein OS=Macrostomum lign...	[more]
A0A1I8I308	3.338e-41	34.06	RIIa domain-containing protein OS=Macrostomum lign...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSAMXT00000046795.1	3.507e-23	65.71	pep primary_assembly:Astyanax_mexicanus-2.0:2:2232...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Sperm surface protein Sp17 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO46037	1.177e-25	44.93	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35043	6.507e-15	56.45	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSORLT00000038228.1	1.784e-16	55.71	sperm autoantigenic protein 17 [Source:NCBI gene;A...	[more]
ENSORLT00000038630.1	1.800e-16	55.71	sperm autoantigenic protein 17 [Source:NCBI gene;A...	[more]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000046919.1	5.055e-9	79.11	SMESG000046919.1	[more]
SMESG000046919.1	2.581e-176	69.25	SMESG000046919.1	[more]
SMESG000046919.1	6.071e-175	69.68	SMESG000046919.1	[more]
SMESG000046919.1	5.378e-168	62.90	SMESG000046919.1	[more]
SMESG000038137.1	1.744e-7	25.48	SMESG000038137.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30007142 ID=SMED30007142|Name=Sperm surface protein Sp17|organism=Schmidtea mediterranea sexual|type=transcript|length=1792bp
GTTGCTTAGTTACAAATAGTAACGTAAATTAATATCGAAAAATTTGTTGA
TTACTTATTAAAAGATTTTTATAATTAAATATGGCAGTTCCATATTCAAA
CACAAGATTAAGAGTGCCAAAAGGTTTTCAAAATGTGTTAGAAGGTCTTG
CACGTGAAGTCTTAAGATGCCAACCAGAAAATATTTATAGTTTTGCTGCT
GATTATTTTGAAGAGTTATTAAGAATGCGTCAAGAAACTGGTTATGACGA
TATAGCACTGTTAGGAGCGAAATTAGAAGACAGATTTTATAATAATCATA
GTTTTAAAAATCCTAATACAAATACAAATAATGAAGAACAACAATCGGCT
GCTGTTAAAATTCAATCGGAATACCGTCGATTTGCCGCGAAAAAAGAAGT
CGATACGAAGAAAGAAGAAGAGGCTGCAATCGTTATACAAGCTTCATTCA
GAGGGCATTTTGAAAGGCAGAACCAAAAACCAAATTCAAATGAAGAAATT
GATAGAATTCCTTCCGATGCTCAAACTAATCAGCACAATAATACAGATAT
AGAAGAAAATACATTGGTACATTCAAACATTGATGAGCCAGAAATTATGG
ATAAACATGAATCTGAAGATCTTCCTCCGGCACATGGTGAAAATCAAAAT
TATCAAGAATATGATATAGAAGACTCTCAAACTATGCTCGATGAATCTAA
ATCAAATCTCAAAGAAAACGACATGAAGCCTGAGGAGCTTCAAAATTCTG
AAGAAATTATTGACATAGATCTTACTGATCCCGATGTTGAAAAAGCTGCG
GTAAAAATCCAATCTGCCTTCAAAGGATTCAAAACTCGCAAAGCAAATGA
GGATACTCCAATAGCTCCGGTAACTAACGATGAAGAAAATCCTGAGCCTA
AAAGACTTCAAGAAATAAAAGAAGATGAAATAATTGATATAGATTTAACC
GATCCAGAGGTTGAGAAGGCAACAATAAAAATACAGTCGACATTTAAAGG
TTTTCGAAGTCGAAAGACATTAGAGGAAATGCAAACTGGAGATGAATCTC
AACATCTTGAAATAGTCGAGGAAAATCTTGAAAAAAGTGATAAAGTTAAA
GCATTCATCGATGAAGAGATCATTGATATAGATCTGAACGATCCTGACGT
AGAGAAAGCAGCTGTCAAAATACAATCGGTTTATAAAGGTTTTCGAAATC
GTAAAACTATTAAGGGACCAGTAGCTGGCGGCCATGGAGATTATGTTGAA
ACGCACGAAAAAGTTAATGAAGATTTAGAATTCGATCACGAAAATCAAGA
AGTTCCAACAGATGAGATTACCAATGTTACAGATAACATGATAAATGAAG
CAGAACAGACTCACGAAGACAATCCGGAATCAGGAGGCCCCACAAACGAC
ACAGATGAGGCTGCGACAAGTCGTACGGAACCAGATGAATAATGATGGAC
ATTTTTTATGAGTTTTCAATTATTTAAGACAGTATTGTTAAGAAAGTGGT
CACTTGTTATTTTAATAATATTGACAATAAATGAGATTATTGGATACGAG
GGAGGCTGACATGATATTTAGATTTTTTTTAGTTAGAAATTTTACTCGCA
TTTAGTTAGTAATATTTTTTACGAGTTTTCCTTTCGAGAAATATTGCAAA
TCCTTAATGACAGTGAATACCTTTTCACATATTACTTTGCCGGTTTCCTT
TTCGCTCTGGTTTCCTTATTTTAGAATTTACTCATACAACTATTTCATGT
CTAAACATTTTGTGTACAACTATAAATTTGCTAAGAATAGAC

protein sequence of SMED30007142-orf-1

>SMED30007142-orf-1 ID=SMED30007142-orf-1|Name=SMED30007142-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=454bp
MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMR
QETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRR
FAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDRIPSDAQTN
QHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQ
TMLDESKSNLKENDMKPEELQNSEEIIDIDLTDPDVEKAAVKIQSAFKGF
KTRKANEDTPIAPVTNDEENPEPKRLQEIKEDEIIDIDLTDPEVEKATIK
IQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFIDEEIIDI
DLNDPDVEKAAVKIQSVYKGFRNRKTIKGPVAGGHGDYVETHEKVNEDLE
FDHENQEVPTDEITNVTDNMINEAEQTHEDNPESGGPTNDTDEAATSRTE
PDE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0005777	peroxisome

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000070	intestinal phagocyte
PLANA:0000099	neuron
PLANA:0000106	ciliated epithelial cell
PLANA:0000418	head
PLANA:0002032	epidermal cell
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR003117	cAMP_dep_PK_reg_su_I/II_a/b
IPR000048	IQ_motif_EF-hand-BS

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003117	cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain	SMART	SM00394	akam_3	coord: 14..51 e-value: 4.1E-13 score: 59.6
IPR003117	cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain	PFAM	PF02197	RIIa	coord: 14..50 e-value: 5.1E-14 score: 51.7
IPR000048	IQ motif, EF-hand binding site	SMART	SM00015	iq_5	coord: 235..257 e-value: 0.29 score: 20.2 coord: 293..315 e-value: 0.027 score: 23.6 coord: 111..133 e-value: 3.3E-4 score: 30.0 coord: 86..108 e-value: 0.9 score: 18.6 coord: 356..378 e-value: 0.12 score: 21.5
IPR000048	IQ motif, EF-hand binding site	PFAM	PF00612	IQ	coord: 295..313 e-value: 5.6E-4 score: 19.5 coord: 114..131 e-value: 0.0026 score: 17.4 coord: 358..376 e-value: 0.0014 score: 18.2 coord: 89..106 e-value: 0.043 score: 13.6 coord: 237..254 e-value: 1.9E-4 score: 20.9
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 295..323 score: 9.359
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 237..265 score: 7.455
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 87..116 score: 7.821
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 112..141 score: 9.304
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 358..386 score: 7.895
None	No IPR available	GENE3D	G3DSA:1.20.890.10		coord: 8..51 e-value: 4.1E-14 score: 53.7
None	No IPR available	GENE3D	G3DSA:1.20.5.190		coord: 87..131 e-value: 1.9E-12 score: 48.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 125..164
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 141..161
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 400..453
None	No IPR available	PANTHER	PTHR10699	NEUROMODULIN	coord: 1..95 coord: 265..322 coord: 336..447 coord: 207..257
None	No IPR available	CDD	cd12100	DD_CABYR_SP17	coord: 12..50 e-value: 4.78231E-19 score: 78.0461
None	No IPR available	SUPERFAMILY	SSF47391	Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit	coord: 8..50

Main menu

Sperm surface protein Sp17

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 48

Owlery

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