SMED30007111
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007111 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14Note: Hover over icons to view figure legend
Alignments
SMED30007111 aligns in the following genomic locations:
Homology
BLAST of SMED30007111 vs. TrEMBL
Match: A0A504YHQ6 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_00202 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 3.591e-14 Identity = 42/117 (35.90%), Postives = 66/117 (56.41%), Query Frame = 1 Query: 1672 QVDHSEHAEPRKYLTMKQLMHCRMTSRPCCIPVHEWSKVAIAGTRRNLRPELFCSYHKNSHCAYQVLAHKYLHRHGISIAKHLERYEILEIINKSVQENAYIRIQNSLLKCLMRVLE 2022 + D +EH E MH R + RP +P +W + ++ + L+ + F K HC + +L K H+ G+SIA HL R E++E INKS+Q+ AY RIQ S+ +CL +V++ Sbjct: 2185 KADAAEHEE---------WMHTRGSPRPSGVPPGQWRLLGMSKGLKKLQTDNFHQIFKGVHCRHALLVQKGFHKQGLSIAAHLARMELVECINKSIQQRAYRRIQTSMKECLKKVIQ 2292
BLAST of SMED30007111 vs. TrEMBL
Match: G7YSZ3 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_109827 PE=4 SV=1) HSP 1 Score: 85.1149 bits (209), Expect = 4.512e-13 Identity = 59/189 (31.22%), Postives = 94/189 (49.74%), Query Frame = 1 Query: 1459 KNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVK--AIMENQKIXXXXXXXXXXXXXXXXGSKKITEVMQVDHSEHAEPRKYLTMKQLMHCRMTSRPCCIPVHEWSKVAIAGTRRNLRPELFCSYHKNSHCAYQVLAHKYLHRHGISIAKHLERYEILEIINKSVQENAYIRIQNSLLKCLMRVL 2019 + +T LP I + L N LD R L + PG L + A + ++K + + ++ +S + E Q + A+ QL R++SRP IP+ +W + I ++F S + HC Y LA K H+ +SIA HL R E++ INK++QE AY RIQ+S+ +CL +V+ Sbjct: 2089 RKSTDLPTITSVEKLTAN-LDTVTRYLKSVMPPGNLTRLLAQLRSEKTSAEAVAELESA---------LIEATQKARTSEAD-------AQLDFVRLSSRPSKIPLSQWKVLDIPQKIERSPSDMFQSETQGVHCRYAQLARKGFHKQDLSIAAHLMRMELVNCINKAIQERAYQRIQSSMRECLKKVI 2260
BLAST of SMED30007111 vs. TrEMBL
Match: A0A4S2L931 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009011 PE=4 SV=1) HSP 1 Score: 83.9593 bits (206), Expect = 9.540e-13 Identity = 39/99 (39.39%), Postives = 59/99 (59.60%), Query Frame = 1 Query: 1723 QLMHCRMTSRPCCIPVHEWSKVAIAGTRRNLRPELFCSYHKNSHCAYQVLAHKYLHRHGISIAKHLERYEILEIINKSVQENAYIRIQNSLLKCLMRVL 2019 Q R++SRP IP+ +W + I ++F S + HC Y LA K H+ +SIA HL R E++ INK++QE AY RIQ+S+++CL +V+ Sbjct: 2115 QTEFVRLSSRPSKIPLSQWYVLDIPQKIERSPSDMFQSETQGVHCRYAQLARKGFHKQDLSIAAHLTRMELMNCINKAIQERAYRRIQSSMMECLKKVI 2213
BLAST of SMED30007111 vs. TrEMBL
Match: A0A1S8WUT2 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_05859 PE=4 SV=1) HSP 1 Score: 69.707 bits (169), Expect = 2.501e-8 Identity = 52/171 (30.41%), Postives = 79/171 (46.20%), Query Frame = 1 Query: 1459 KNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVK--AIMENQKIXXXXXXXXXXXXXXXXGSKKITEVMQVDHSEHAEPRKYLTMKQLMHCRMTSRPCCIPVHEWSKVAIAGTRRNLRPELFCSYHKNSHCAYQVLAHKYLHRHGISIAKHLERYEILEIINKSVQE 1965 K +T LP I + L N LD R L + PG L + A + N+K + + ++ E +D ++ A R QL R++SRP IP+ +W + I ++F S + HC Y LA K H+ +SIA HL R E++ INK++QE Sbjct: 1236 KKSTDLPTITSVEELTAN-LDTVTRYLKSVMPPGNLTRLLAQLRNEKTSAEAVAEL--------------EGALIDAAQKA--RNSEADAQLDFVRLSSRPSKIPLSQWKVLDIPQKIERGPSDMFQSETQGVHCRYAQLARKGFHKQDLSIAAHLTRMELVNCINKAIQE 1389
BLAST of SMED30007111 vs. TrEMBL
Match: A0A075ABZ3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_12614 PE=4 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 3.811e-8 Identity = 53/176 (30.11%), Postives = 80/176 (45.45%), Query Frame = 1 Query: 1459 KNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVK--AIMENQKIXXXXXXXXXXXXXXXXGSKKITEVMQVDHSEHAEPRKYLTMKQLMHCRMTSRPCCIPVHEWSKVAIAGTRRNLRPELFCSYHKNSHCAYQVLAHKYLHRHGISIAKHLERYEILEIINKSVQENAYIR 1980 K +T LP I + L N LD R L + PG L + A + N+K + + ++ E +D ++ A R QL R++SRP IP+ +W + I ++F S + HC Y LA K H+ +SIA HL R E++ INK++QE R Sbjct: 2188 KKSTDLPTITSVEELTAN-LDTVTRYLKSVMPPGNLTRLLAQLRNEKTSAEAVAEL--------------EGALIDAAQKA--RNSEADAQLDFVRLSSRPSKIPLSQWKVLDIPQKIERGPSDMFQSETQGVHCRYAQLARKGFHKQDLSIAAHLTRMELVNCINKAIQERVSRR 2346
BLAST of SMED30007111 vs. Planmine SMEST
Match: SMESG000018075.1 (SMESG000018075.1) HSP 1 Score: 528.865 bits (1361), Expect = 0.000e+0 Identity = 276/277 (99.64%), Postives = 276/277 (99.64%), Query Frame = 1 Query: 877 MNPLMDFSGYPSVVSKSKPESLVSYVSRPGSPSIKLSKNSVLPPIGKSKRKLNDMILQNAHRPDYFHKIEMLEDHDKAKQREEKRASRARKLSQLKKIAKNLKYKVSTEMKVHAKVERRLTNYGQPDIAELIAKKTEYYGSIDALRTKIKDEISKEYSNFVKRIDFDASDSQQESNMMSEPALIQTKKFQKFYGKNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVKAIMENQKIXXXXXXXXXXXXXXXXGSKKITEVMQVDHSEHAEPRK 1707 MNPLMDFSGYPSVVSKSKPESLVSYVSRPGSPSIKLSKNSVLPPIGKSKRKLNDMILQNAHRPDYFHKIEMLEDHDKAKQREEKRASRARKLSQLKKIAKNLKYKVSTEMKVHAKVERRLTNYGQPDIAELIAKKTEYYGSIDALRTKIKDEISKEYSNFVKRIDFDASDSQQESNMMSEPALIQTKKFQKFYGKNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVKAIMENQKI TSSDSKYDSDDWWTDGSKKITEVMQVDHSEHAEPRK Sbjct: 1 MNPLMDFSGYPSVVSKSKPESLVSYVSRPGSPSIKLSKNSVLPPIGKSKRKLNDMILQNAHRPDYFHKIEMLEDHDKAKQREEKRASRARKLSQLKKIAKNLKYKVSTEMKVHAKVERRLTNYGQPDIAELIAKKTEYYGSIDALRTKIKDEISKEYSNFVKRIDFDASDSQQESNMMSEPALIQTKKFQKFYGKNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVKAIMENQKIATSSDSKYDSDDWWTDGSKKITEVMQVDHSEHAEPRK 277
BLAST of SMED30007111 vs. Planmine SMEST
Match: SMESG000018075.1 (SMESG000018075.1) HSP 1 Score: 519.62 bits (1337), Expect = 0.000e+0 Identity = 272/273 (99.63%), Postives = 272/273 (99.63%), Query Frame = 1 Query: 889 MDFSGYPSVVSKSKPESLVSYVSRPGSPSIKLSKNSVLPPIGKSKRKLNDMILQNAHRPDYFHKIEMLEDHDKAKQREEKRASRARKLSQLKKIAKNLKYKVSTEMKVHAKVERRLTNYGQPDIAELIAKKTEYYGSIDALRTKIKDEISKEYSNFVKRIDFDASDSQQESNMMSEPALIQTKKFQKFYGKNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVKAIMENQKIXXXXXXXXXXXXXXXXGSKKITEVMQVDHSEHAEPRK 1707 MDFSGYPSVVSKSKPESLVSYVSRPGSPSIKLSKNSVLPPIGKSKRKLNDMILQNAHRPDYFHKIEMLEDHDKAKQREEKRASRARKLSQLKKIAKNLKYKVSTEMKVHAKVERRLTNYGQPDIAELIAKKTEYYGSIDALRTKIKDEISKEYSNFVKRIDFDASDSQQESNMMSEPALIQTKKFQKFYGKNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVKAIMENQKI TSSDSKYDSDDWWTDGSKKITEVMQVDHSEHAEPRK Sbjct: 1 MDFSGYPSVVSKSKPESLVSYVSRPGSPSIKLSKNSVLPPIGKSKRKLNDMILQNAHRPDYFHKIEMLEDHDKAKQREEKRASRARKLSQLKKIAKNLKYKVSTEMKVHAKVERRLTNYGQPDIAELIAKKTEYYGSIDALRTKIKDEISKEYSNFVKRIDFDASDSQQESNMMSEPALIQTKKFQKFYGKNTTLLPFIGKSKSLVGNVLDQGLRALHFNLGPGTLVKAIMENQKIATSSDSKYDSDDWWTDGSKKITEVMQVDHSEHAEPRK 273
BLAST of SMED30007111 vs. Planmine SMEST
Match: SMESG000027823.1 (SMESG000027823.1) HSP 1 Score: 307.76 bits (787), Expect = 2.465e-100 Identity = 202/206 (98.06%), Postives = 203/206 (98.54%), Query Frame = 1 Query: 148 DPLVDNEEIMEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNXXXXXXXXXXXXXXFKDEIQTFSPLYPDEVDFSKNPLSQLTIGEHXXXXXXXXXXXXXXXXFLRNVNTLKPMDQEKGYEMGLMIRHSKSDDDNLAITSVGSSKKLASPIYPTMFNVTKAQSKKLNADYSXXXXXXXXXXXXXNVIKMHERKIDMMIHT 765 DPLVDNEEIMEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNLLLLLDIGVEDLDDFKDEIQTFSPLYPDEVDFSKNPLSQLTIGEHKLKISKNDIIIDDKLNFLRNVNTLKPMDQEKGYEMGLMIRHSKSDD NLAITSVGSSKKLASPIYPTMFNVTKAQSKKLNADYSKKEELKLKITEIENVIKMHERKIDM+ T Sbjct: 27 DPLVDNEEIMEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNLLLLLDIGVEDLDDFKDEIQTFSPLYPDEVDFSKNPLSQLTIGEHKLKISKNDIIIDDKLNFLRNVNTLKPMDQEKGYEMGLMIRHSKSDDGNLAITSVGSSKKLASPIYPTMFNVTKAQSKKLNADYSKKEELKLKITEIENVIKMHERKIDMVSFT 232
BLAST of SMED30007111 vs. Planmine SMEST
Match: SMESG000027823.1 (SMESG000027823.1) HSP 1 Score: 290.426 bits (742), Expect = 3.441e-94 Identity = 193/197 (97.97%), Postives = 194/197 (98.48%), Query Frame = 1 Query: 175 MEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNXXXXXXXXXXXXXXFKDEIQTFSPLYPDEVDFSKNPLSQLTIGEHXXXXXXXXXXXXXXXXFLRNVNTLKPMDQEKGYEMGLMIRHSKSDDDNLAITSVGSSKKLASPIYPTMFNVTKAQSKKLNADYSXXXXXXXXXXXXXNVIKMHERKIDMMIHT 765 MEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNLLLLLDIGVEDLDDFKDEIQTFSPLYPDEVDFSKNPLSQLTIGEHKLKISKNDIIIDDKLNFLRNVNTLKPMDQEKGYEMGLMIRHSKSDD NLAITSVGSSKKLASPIYPTMFNVTKAQSKKLNADYSKKEELKLKITEIENVIKMHERKIDM+ T Sbjct: 1 MEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNLLLLLDIGVEDLDDFKDEIQTFSPLYPDEVDFSKNPLSQLTIGEHKLKISKNDIIIDDKLNFLRNVNTLKPMDQEKGYEMGLMIRHSKSDDGNLAITSVGSSKKLASPIYPTMFNVTKAQSKKLNADYSKKEELKLKITEIENVIKMHERKIDMVSFT 197 The following BLAST results are available for this feature:
BLAST of SMED30007111 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30007111 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30007111 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30007111 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30007111 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30007111 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007111 ID=SMED30007111|Name=SMED30007111|organism=Schmidtea mediterranea sexual|type=transcript|length=2082bpback to top protein sequence of SMED30007111-orf-1 >SMED30007111-orf-1 ID=SMED30007111-orf-1|Name=SMED30007111-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=619bp MEMIASNSFSNLITDMQNILSQSLKQYDIPISLDRSTTNLLLLLDIGVEDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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