Myosin light chain 1

Overview
NameMyosin light chain 1
Smed IDSMED30007110
Length (bp)349
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Myosin light chain 1 (SMED30007110) t-SNE clustered cells

Violin plots show distribution of expression levels for Myosin light chain 1 (SMED30007110) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myosin light chain 1 (SMED30007110) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myosin light chain 1 (SMED30007110) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007110

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30007110 SmedASXL_005380SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30007110 dd_Smed_v4_4478_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30007110 dd_Smed_v4_4478_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007110 dd_Smed_v4_4478_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30007110 dd_Smed_v6_4478_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Human
Match: CALML5 (calmodulin like 5 [Source:HGNC Symbol;Acc:HGNC:18180])

HSP 1 Score: 47.3654 bits (111), Expect = 3.512e-7
Identity = 22/63 (34.92%), Postives = 42/63 (66.67%), Query Frame = 3 
Query:   63 EMDFHKLFTMIDQDSSGSISINELKSALKQSGE---EFLMDEILNGLDINGDGEITYMEYCSA 242
            E  + K F+ +D D +G+I+  EL +ALK +G+   E  + ++++ +D +GDGEI++ E+ +A
Sbjct:   10 EAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTA 72
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9])

HSP 1 Score: 45.4394 bits (106), Expect = 8.222e-7
Identity = 22/65 (33.85%), Postives = 42/65 (64.62%), Query Frame = 3 
Query:   69 DFHKLFTMIDQDSSGSISINELKSALKQSGE---EFLMDEILNGLDINGDGEITYMEYCSALDLI 254
            +F + F + D+D +G+ISI EL  A++  G+   E  M EI++ +D++G+G++ + E+C  +  I
Sbjct:   30 EFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVDLDGNGQVEFPEFCVMMKRI 94
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Celegans
Match: cal-3 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:U4PQW9])

HSP 1 Score: 45.8246 bits (107), Expect = 8.659e-7
Identity = 22/65 (33.85%), Postives = 42/65 (64.62%), Query Frame = 3 
Query:   69 DFHKLFTMIDQDSSGSISINELKSALKQSGE---EFLMDEILNGLDINGDGEITYMEYCSALDLI 254
            +F + F + D+D +G+ISI EL  A++  G+   E  M EI++ +D++G+G++ + E+C  +  I
Sbjct:  100 EFKEAFLLFDKDGNGTISIKELGVAMRALGQNPTEQQMMEIIHDVDLDGNGQVEFPEFCVMMKRI 164
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 45.4394 bits (106), Expect = 8.808e-7
Identity = 23/62 (37.10%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:   69 DFHKLFTMIDQDSSGSISINELKSALKQSGEEFLMDEIL---NGLDINGDGEITYMEYCSAL 245
            +F + F M D+D +G+IS  EL  A++  G+     EIL   N +DI+G+G+I + E+C  +
Sbjct:   25 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMM 86
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 45.4394 bits (106), Expect = 9.184e-7
Identity = 23/62 (37.10%), Postives = 38/62 (61.29%), Query Frame = 3 
Query:   69 DFHKLFTMIDQDSSGSISINELKSALKQSGEEFLMDEIL---NGLDINGDGEITYMEYCSAL 245
            +F + F M D+D +G+IS  EL  A++  G+     EIL   N +DI+G+G+I + E+C  +
Sbjct:   44 EFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEFCVMM 105
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Zebrafish
Match: calm3a (calmodulin 3a (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030131-5590])

HSP 1 Score: 43.5134 bits (101), Expect = 7.662e-6
Identity = 26/75 (34.67%), Postives = 40/75 (53.33%), Query Frame = 3 
Query:   63 EMDFHKLFTMIDQDSSGSISINELKSALKQSGEEFL---MDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRK 278
            E +  + F + D+D +G IS  EL+  +   GE+     +DE++   DI+GDG++ Y   CSA   I   GL  K
Sbjct:   83 EEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEGECSAAIHIACVGLRLK 157
[Close]
BLAST of Myosin light chain 1 vs. UniProt/SwissProt
Match: sp|Q38868|CDPK9_ARATH (Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 1.270e-7
Identity = 29/74 (39.19%), Postives = 47/74 (63.51%), Query Frame = 3 
Query:   30 TRHLQLLQSIYEMDFHKLFTMIDQDSSGSISINELKSALKQS--GEEFLMDEILNGLDINGDGEITYMEYCSAL 245
            T H   L+S    + +K F   D+DSSG I+I+EL+SALK+   G++  + E+L+ +D + DG I Y E+C+ +
Sbjct:  457 TMHRHRLES--NENLYKAFQHFDKDSSGYITIDELESALKEYGMGDDATIKEVLSDVDSDNDGRINYEEFCAMM 528
[Close]
BLAST of Myosin light chain 1 vs. UniProt/SwissProt
Match: sp|Q2QVG8|CDPKT_ORYSJ (Calcium-dependent protein kinase 29 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK29 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 6.145e-7
Identity = 25/66 (37.88%), Postives = 42/66 (63.64%), Query Frame = 3 
Query:   36 HLQLLQSIYEMDFHKLFTMIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEY 233
            HL+ ++S  +    K+F+  D++ SG I I ELK AL   G++  +D+I   +DI+ DG+I+Y E+
Sbjct:  452 HLKKIRS--DEHLPKVFSFFDKNGSGYIEIEELKEALSPRGDQKSIDDIFLDVDIDKDGKISYEEF 515
[Close]
BLAST of Myosin light chain 1 vs. UniProt/SwissProt
Match: sp|Q2QX45|CDPKS_ORYSJ (Calcium-dependent protein kinase 28 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK28 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.577e-6
Identity = 24/58 (41.38%), Postives = 38/58 (65.52%), Query Frame = 3 
Query:   78 KLFTMIDQDSSGSISINELKSALKQSGEEFLMDEI---LNGLDINGDGEITYMEYCSA 242
            +LF MID D+SG+I+ +ELK+ LK+ G + +  EI   ++  DI+  G I Y E+ +A
Sbjct:  357 ELFKMIDTDNSGTITYDELKNGLKRVGSDLMEPEIQALMDAADIDNSGTIDYGEFLAA 414
[Close]
BLAST of Myosin light chain 1 vs. UniProt/SwissProt
Match: sp|Q9NZT1|CALL5_HUMAN (Calmodulin-like protein 5 OS=Homo sapiens OX=9606 GN=CALML5 PE=1 SV=2)

HSP 1 Score: 47.3654 bits (111), Expect = 1.687e-6
Identity = 22/63 (34.92%), Postives = 42/63 (66.67%), Query Frame = 3 
Query:   63 EMDFHKLFTMIDQDSSGSISINELKSALKQSGE---EFLMDEILNGLDINGDGEITYMEYCSA 242
            E  + K F+ +D D +G+I+  EL +ALK +G+   E  + ++++ +D +GDGEI++ E+ +A
Sbjct:   10 EAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTA 72
[Close]
BLAST of Myosin light chain 1 vs. UniProt/SwissProt
Match: sp|Q9C6P3|CDPKX_ARATH (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 3.292e-6
Identity = 27/74 (36.49%), Postives = 45/74 (60.81%), Query Frame = 3 
Query:   30 TRHLQLLQSIYEMDFHKLFTMIDQDSSGSISINELKSALKQSG--EEFLMDEILNGLDINGDGEITYMEYCSAL 245
            T H   L+S    + +K F   D+D SG I+ +EL++ALK+ G  ++  + EIL+ +D + DG I Y E+C+ +
Sbjct:  439 TMHRHRLES--NENVYKAFQHFDKDGSGYITTDELEAALKEYGMGDDATIKEILSDVDADNDGRINYDEFCAMM 510
[Close]
BLAST of Myosin light chain 1 vs. TrEMBL
Match: A0A1I8HCC1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017071g1 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.169e-8
Identity = 34/79 (43.04%), Postives = 46/79 (58.23%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKSALKQSG-EEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSHKIGQ 299
            MDFH+ F  ID D SG IS+ EL+   K+   ++  + + LN  D +  G ITY EYC+ L LIP   +V K  HK+ Q
Sbjct:    5 MDFHQAFQAIDTDHSGEISVEELEDYCKKMNYKDTFVKKWLNLFDEDESGTITYDEYCNTLGLIPKQEVVEK--HKVQQ 81
[Close]
BLAST of Myosin light chain 1 vs. TrEMBL
Match: A0A1I8I3J0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017071g2 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.169e-8
Identity = 34/79 (43.04%), Postives = 46/79 (58.23%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKSALKQSG-EEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSHKIGQ 299
            MDFH+ F  ID D SG IS+ EL+   K+   ++  + + LN  D +  G ITY EYC+ L LIP   +V K  HK+ Q
Sbjct:    5 MDFHQAFQAIDTDHSGEISVEELEDYCKKMNYKDTFVKKWLNLFDEDESGTITYDEYCNTLGLIPKQEVVEK--HKVQQ 81
[Close]
BLAST of Myosin light chain 1 vs. TrEMBL
Match: A0A1I8IIW6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.621e-8
Identity = 35/79 (44.30%), Postives = 47/79 (59.49%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKS-ALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSHKIGQ 299
            MDFHK F  ID D SG I+++EL+S   K + +E  + + L   D + +G ITY EYC+AL L P   +V K  HK  Q
Sbjct:    9 MDFHKAFLSIDTDQSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVVAK--HKSQQ 85
[Close]
BLAST of Myosin light chain 1 vs. TrEMBL
Match: A0A267FI43 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004334g1 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.633e-8
Identity = 35/79 (44.30%), Postives = 47/79 (59.49%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKS-ALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSHKIGQ 299
            MDFHK F  ID D SG I+++EL+S   K + +E  + + L   D + +G ITY EYC+AL L P   +V K  HK  Q
Sbjct:    9 MDFHKAFLSIDTDQSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVVAK--HKSQQ 85
[Close]
BLAST of Myosin light chain 1 vs. TrEMBL
Match: A0A1I8FHH7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 7.004e-8
Identity = 34/79 (43.04%), Postives = 47/79 (59.49%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKS-ALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSHKIGQ 299
            MDFHK F  ID D SG I+++EL+S   K + +E  + + L   D + +G ITY EYC+AL L P   ++ K  HK  Q
Sbjct:    9 MDFHKAFLSIDTDHSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVIAK--HKSQQ 85
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Yeast
Match: MLC1 (Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition [Source:SGD;Acc:S000003074])

HSP 1 Score: 42.743 bits (99), Expect = 1.180e-6
Identity = 20/59 (33.90%), Postives = 38/59 (64.41%), Query Frame = 3 
Query:   69 DFHKLFTMIDQDSSGSISINELKSALKQSGEEFL---MDEILNGLDINGDGEITYMEYC 236
            DF K F + D++S+G +S+ +L+  L   GE+     +DE+L G++++ +GEI Y ++ 
Sbjct:   85 DFVKAFQVFDKESTGKVSVGDLRYMLTGLGEKLTDAEVDELLKGVEVDSNGEIDYKKFI 143
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Nematostella
Match: EDO39381 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAE1])

HSP 1 Score: 54.6842 bits (130), Expect = 4.917e-10
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKSALKQSGEEF---LMDEILNGLDINGDGEITYMEYCSALDLIPDPGL---VRKFSHKIG 296
            +   K+F  ID+D+SG I  NE+ +ALK+         +D IL G+D+N D  I + E+C+    IP P L    +K+S  +G
Sbjct:   60 LSLEKIFQEIDEDNSGYIDHNEISNALKKLDIHLPSRQIDGILKGMDLNNDNRIDFDEFCAFFSDIPSPNLQLIAKKWSSGVG 142
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Nematostella
Match: EDO29140 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4H4])

HSP 1 Score: 53.5286 bits (127), Expect = 1.197e-9
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKSALKQSGEEF---LMDEILNGLDINGDGEITYMEYCSALDLIPDPGL---VRKFSHKIG 296
            +   K+F  ID+D+SG I  NE+ +ALK+         +D IL G+D+N D  I + E+C+    IP P L    +K+S  +G
Sbjct:   68 LRLEKIFKEIDEDNSGYIDHNEISNALKKLDINLPSRQIDGILKGMDLNNDNRIDFDEFCAFFSDIPSPNLQLIAKKWSSGVG 150
[Close]
BLAST of Myosin light chain 1 vs. Ensembl Nematostella
Match: EDO48530 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ74])

HSP 1 Score: 41.2022 bits (95), Expect = 9.495e-6
Identity = 18/51 (35.29%), Postives = 33/51 (64.71%), Query Frame = 3 
Query:   81 LFTMIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEY 233
            +F   D+D+SGSI   E K  + ++G +  +D+ L  LD +G+G+I++ E+
Sbjct:   84 MFKKYDKDASGSIDREEFKQLMHETGNKVNIDKALKKLDKDGNGQISFTEF 134
[Close]
BLAST of Myosin light chain 1 vs. Planmine SMEST
Match: SMESG000018597.1 (SMESG000018597.1)

HSP 1 Score: 139.428 bits (350), Expect = 1.366e-44
Identity = 69/70 (98.57%), Postives = 69/70 (98.57%), Query Frame = 3 
Query:   90 MIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSHKIGQ 299
            MIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFS KIGQ
Sbjct:    1 MIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKFSRKIGQ 70
[Close]
BLAST of Myosin light chain 1 vs. Planmine SMEST
Match: SMESG000018602.1 (SMESG000018602.1)

HSP 1 Score: 54.6842 bits (130), Expect = 3.890e-11
Identity = 26/72 (36.11%), Postives = 40/72 (55.56%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKF 281
            MD H++F  ID D +G I+  E   A K  G+E  ++ +    D NG   ITY +YCSA+ + P+  + + F
Sbjct:    1 MDRHQIFVKIDHDKNGLITKKEFDEAQKSFGKEETIEILKKCFDSNGGSSITYADYCSAVGISPNASIQKSF 72
[Close]
BLAST of Myosin light chain 1 vs. Planmine SMEST
Match: SMESG000019835.1 (SMESG000019835.1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.882e-9
Identity = 26/64 (40.62%), Postives = 39/64 (60.94%), Query Frame = 3 
Query:   69 DFHKLFTMIDQDSSGSISINELKS-ALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIP 257
            DFHK F  ID+D SG I+++EL+   +K + ++  + + L   D N  G+ITY  YC  L L+P
Sbjct:    3 DFHKAFEAIDEDGSGEITVDELQHYMIKNNYKDSFVSKWLTLFDSNQSGKITYKHYCDTLGLVP 66
[Close]
BLAST of Myosin light chain 1 vs. Planmine SMEST
Match: SMESG000078154.1 (SMESG000078154.1)

HSP 1 Score: 49.2914 bits (116), Expect = 8.508e-8
Identity = 27/74 (36.49%), Postives = 41/74 (55.41%), Query Frame = 3 
Query:   39 LQLLQSIYEMDFHKLFTMIDQDSSGSISINELKSALKQSG-EEFLMDEILNGLDINGDGEITYMEYCSALDLIP 257
            L++ + +  +DF K F  ID D SG IS  E+ + +++   E   +D+ L   + N DG IT   YC +L LIP
Sbjct:  233 LKISKKVMSLDFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIP 306
[Close]
BLAST of Myosin light chain 1 vs. Planmine SMEST
Match: SMESG000018598.1 (SMESG000018598.1)

HSP 1 Score: 46.2098 bits (108), Expect = 8.667e-8
Identity = 24/72 (33.33%), Postives = 37/72 (51.39%), Query Frame = 3 
Query:   66 MDFHKLFTMIDQDSSGSISINELKSALKQSGEEFLMDEILNGLDINGDGEITYMEYCSALDLIPDPGLVRKF 281
            MD H  F  ID + +G I++ E + A K  G+    + I    + N    ITY E+C+   + PD G ++KF
Sbjct:    1 MDHHATFLKIDSNKNGIITLKEFEDAKKSIGKA-DAEAIRKQFESNSGNNITYAEFCNLFGIAPDDGTMKKF 71
The following BLAST results are available for this feature:
BLAST of Myosin light chain 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CALML53.512e-734.92calmodulin like 5 [Source:HGNC Symbol;Acc:HGNC:181... [more]
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BLAST of Myosin light chain 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
cal-38.222e-733.85CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
cal-38.659e-733.85CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
cal-18.808e-737.10Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-19.184e-737.10Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
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BLAST of Myosin light chain 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myosin light chain 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
calm3a7.662e-634.67calmodulin 3a (phosphorylase kinase, delta) [Sourc... [more]
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BLAST of Myosin light chain 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myosin light chain 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myosin light chain 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q38868|CDPK9_ARATH1.270e-739.19Calcium-dependent protein kinase 9 OS=Arabidopsis ... [more]
sp|Q2QVG8|CDPKT_ORYSJ6.145e-737.88Calcium-dependent protein kinase 29 OS=Oryza sativ... [more]
sp|Q2QX45|CDPKS_ORYSJ1.577e-641.38Calcium-dependent protein kinase 28 OS=Oryza sativ... [more]
sp|Q9NZT1|CALL5_HUMAN1.687e-634.92Calmodulin-like protein 5 OS=Homo sapiens OX=9606 ... [more]
sp|Q9C6P3|CDPKX_ARATH3.292e-636.49Calcium-dependent protein kinase 33 OS=Arabidopsis... [more]
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BLAST of Myosin light chain 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HCC12.169e-843.04Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I3J02.169e-843.04Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IIW63.621e-844.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FI433.633e-844.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8FHH77.004e-843.04Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Myosin light chain 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myosin light chain 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myosin light chain 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MLC11.180e-633.90Essential light chain for Myo1p; light chain for M... [more]
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BLAST of Myosin light chain 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO393814.917e-1036.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO291401.197e-936.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485309.495e-635.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Myosin light chain 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myosin light chain 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018597.11.366e-4498.57SMESG000018597.1[more]
SMESG000018602.13.890e-1136.11SMESG000018602.1[more]
SMESG000019835.11.882e-940.63SMESG000019835.1[more]
SMESG000078154.18.508e-836.49SMESG000078154.1[more]
SMESG000018598.18.667e-833.33SMESG000018598.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007110 ID=SMED30007110|Name=Myosin light chain 1|organism=Schmidtea mediterranea sexual|type=transcript|length=349bp
AAATTTTATTTCGGTTTAACTAAATCGATACTCGACATTTACAGCTACTC
CAGTCAATTTATGAAATGGATTTTCATAAACTTTTTACGATGATAGATCA
AGACTCAAGTGGATCAATTTCGATAAATGAATTAAAATCCGCTCTAAAAC
AGTCTGGGGAGGAGTTTTTGATGGATGAAATACTAAATGGTCTGGATATC
AACGGGGATGGAGAAATAACCTACATGGAATATTGTTCGGCACTCGATTT
GATTCCGGATCCAGGATTGGTTAGAAAATTTAGCCACAAAATTGGACAGT
GAACTAATTTTTTTGAACTATTATTTAAATAAAATATAATTGATATTTA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000420parapharyngeal region
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0000166nucleotide binding
GO:0004672protein kinase activity
GO:0004674protein serine/threonine kinase activity
GO:0004683calmodulin-dependent protein kinase activity
GO:0005516calmodulin binding
GO:0005524ATP binding
GO:0009931calcium-dependent protein serine/threonine kinase activity
GO:0016301kinase activity
GO:0016740transferase activity
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005886plasma membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0007165signal transduction
GO:0009738abscisic acid-activated signaling pathway
GO:0016310phosphorylation
GO:0018105peptidyl-serine phosphorylation
GO:0035556intracellular signal transduction
GO:0046777protein autophosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 2..30
e-value: 0.0049
score: 26.1
coord: 35..63
e-value: 0.22
score: 20.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 3..59
e-value: 2.9E-9
score: 37.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..33
score: 12.393
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 36..66
score: 8.962
IPR002048EF-hand domainCDDcd00051EFhcoord: 2..60
e-value: 3.98685E-10
score: 48.3129
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..58
e-value: 9.9E-14
score: 53.1
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 11..23
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 44..56
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 2..61