Rho guanine nucleotide exchange factor 28

Overview
NameRho guanine nucleotide exchange factor 28
Smed IDSMED30007097
Length (bp)3968
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rho guanine nucleotide exchange factor 28 (SMED30007097) t-SNE clustered cells

Violin plots show distribution of expression levels for Rho guanine nucleotide exchange factor 28 (SMED30007097) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rho guanine nucleotide exchange factor 28 (SMED30007097) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rho guanine nucleotide exchange factor 28 (SMED30007097) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30007097

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007097SMESG000021797.1 dd_Smed_v6_5252_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30007097SMESG000021797.1 dd_Smed_v4_5252_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Rho guanine nucleotide exchange factor 28 vs. TrEMBL
Match: A0A4E0RMX9 (Rho guanine nucleotide exchange factor 28 OS=Fasciola hepatica OX=6192 GN=D915_001504 PE=4 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 5.928e-57
Identity = 170/665 (25.56%), Postives = 310/665 (46.62%), Query Frame = 3
Query:  540 VISKGEYLMKY-QWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDK--SNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYE---INSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSV-IKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELK-----------RLSASGRNSCTSTTTVTENDSKRD---QIDGYND-----------------EIL--DTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLC-SSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSK 2411
            ++S+ +YL KY  W  F G E   K+    I RND IWE++    G ++ L+++ DVY   +P   AG   +                   + D+  S++L+   DT+ PGL  L+    ++   L+  H    +  +  +G  L +  +DK  S LS+LYG F F +    +++   K+ P    ++ +C+ DP++ +K+L D    I   W ++   L+ II+ T+N   EV  L   +  ++ L+  AE +  +  +A  L    E  +   +  + +   ++   M +    L+  G ++      G       ++TG+AL NC F L+ + E G    +YQLF   +    P +L WG +YGYFR+   K    F F++++     ++     ST  E   W+  M  GF++W+  + + I+  +  L +    I  +W +T+  ++L++ LD+ L+     R      +++E LK           R +++G N+ +S + ++   S +     + G +                  E+L  +T+I  L+  E ++  L +F        +  + RL F+L  SS A ++RSAS++E RKRP  + ++K ETF+  DS ++   P  +A L      +K+D  R+S +   S+FR  S+
Sbjct:   96 LLSRSDYLQKYPSWLGFCGPENKPKFSPTFIARNDTIWELIFSERGYVDMLLMVHDVYMAPFPQFRAGEYDM-------------------LPDRMSSSSLY---DTLFPGLKELLAAHERLLRPLLALHEQAENHVVESLGPCLVKLFDDKTQSSLSQLYGGFLFAQNRIREELVVCKRCPAIAQFLEQCKHDPRSGRKSLEDCHMVIVQRWTKVETLLDAIIKNTLNRPAEVASLELARNSVRHLLKQAEAMMMRMEHAVKLAQFAELLQAPPVPLENQIETQLLHEMRSPETTLINYGPLRVTPIVAGQQTSNSNEVTGIALNNCFFMLRRMPETG----RYQLFTGTD---MPPVLRWGKVYGYFRKSVEK--VGFGFFILLHS-NAALTLFHCSTVDELARWEQIMNHGFAQWREASPITIEQLDSDLVKTRNAIAERWKHTEAVLDLLRELDRDLEQAWSVRMFACSALIQERLKMQVLDQQIATDRANSNGTNAASSQSDISRPRSSKTPVFPVSGLSPTTGSSCNQSESLAQITMELLHSNTYIDSLTPSEDLDQLLSLF--------RGNLARLSFALVGSSSACLSRSASDVEERKRPPAKPIRKNETFSVRDSISHANDPKTLAELG--RKAKKRDAHRLSTT-MASIFRSASR 717          
BLAST of Rho guanine nucleotide exchange factor 28 vs. TrEMBL
Match: A0A504YTM8 (Rho guanine nucleotide exchange factor 28 OS=Fasciola gigantica OX=46835 GN=FGIG_00188 PE=4 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 4.360e-55
Identity = 168/665 (25.26%), Postives = 308/665 (46.32%), Query Frame = 3
Query:  540 VISKGEYLMKY-QWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDK--SNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYE---INSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSV-IKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKR-----------LSASGRNSCTS--------------------TTTVTENDSKRDQIDGYNDEIL--DTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLC-SSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSK 2411
            ++S+ +YL KY  W  F G E   K+    I RND IWE++    G ++ L+++ DVY   +P   AG   +                   + D+  S++L+   DT+ PGL  L+    ++   L+  H    +  +  +G  L +  +DK  S LS+LYG F F +    +++   K+ P    ++ +C+ DP++ +K+L D    I   W ++   L+ II+ T+N   EV  L   +  ++ L+  AE +  +  +A  L    E  +   +  + +   ++   M +    L+  G ++      G       ++TG+AL NC F L+ + E G    +YQLF   +    P +L WG +YGYFR+   K    F F++++     ++     ST  E   W+  M  GF++W+  + + I+  +  L +    I  +W +T+  ++L++ LD+ L+     R      +++E LK             +++G N+ +S                    + T   + ++ + +     E+L  +T+I  L+  E ++  L +F        +  + RL F+L  SS A ++RSAS++E RKRP  + ++K ETF+  DS ++   P  +A L      +K+D  R+S +   S+FR  S+
Sbjct:   82 LLSRSDYLQKYPSWLGFCGPENKPKFSPTFIARNDTIWELIFSERGYVDMLLMVHDVYMAPFPQFRAGEYDM-------------------LPDRMSSSSLY---DTLFPGLKELLAAHERLLRPLLALHEQAENHVVESLGPCLVKLFDDKTQSSLSQLYGGFLFAQNRIREELVVCKRCPAIAQFLEQCKHDPRSGRKSLEDCHMVIVQRWTKVETLLDAIIKNTLNRPAEVASLELARNSVRHLLKQAEAMMMRMEHAVKLAQFAELLQAPPVPLENQIETQLLHEMRSPETTLINYGPLRVTPIVAGQQTSNSNEVTGIALNNCFFMLRRMPETG----RYQLFTGTD---MPPVLRWGKVYGYFRKSVEK--VGFGFFILLHS-NAALTLFHCSTVDELARWEQIMNHGFAQWREASPITIEQLDSDLVKTRNAIAERWKHTEAILDLLRELDRDLEQAWSVRMFACSALIQERLKMQVLDQQIVTDPANSNGTNAASSQSDISRPRPSKTPVFPMSGLSPTTGSSCNQSESLAQITMELLHSNTYIDSLTPSEDLDQLLSLF--------RGNLARLSFALVGSSSACLSRSASDVEERKRPPAKPIRKNETFSVRDSISHANDPKTLAELG--RKAKKRDAHRLSTT-MASIFRSASR 703          
BLAST of Rho guanine nucleotide exchange factor 28 vs. TrEMBL
Match: A0A1S8X1N0 (DH domain-containing protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_03587 PE=4 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 4.401e-54
Identity = 215/908 (23.68%), Postives = 405/908 (44.60%), Query Frame = 3
Query:  540 VISKGEYLMKY-QWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEI-NSDCKDSERVYEYMTTDNVILVFKGEVQFELRK-GGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSAS-----GRNSCTSTTTVTENDSKR-DQIDGYND------------EILD--TFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCS-SGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKS-------VKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFM--SSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAADDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNIN--QMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITXXXXXXXXXXXDLIEREKKHKEKCELLQRNMDMY----YQMHGINMEEVDL 3146
            ++ K EYL +Y  W  F+G+E  K++    + RND IWE++    G ++ L+++RDVY   +P  E  S     S    R++L                    + + PGLD LIK   +I   L+  H    D+ I  +G  L +  +      L++LYG F F ++   +++    ++P   ++  KC++D ++ +++L D    I   W +    ++ II  T+N+  EV  L   ++ ++ ++  AE++     +A+ L        +   +  +  ++   M + +  L+  G ++  L   G        D+ G+ L +C   LQ + E G    +YQLF+     K P +L WG +YGYFR    K   SF F+++++  E  +     ST  +   W+  +  GFS+WQ   ++  D    L E    I  +W  T+  ++L+  LD+++      R      ++EE+LK L +      G N   S  T + +DS++  +  G +             E+L+  TF+  L     ++    +F +         + RL FSL   S ++++RSAS++E RK    + V+K ETF++ D+  +   P D   L    + +K++  R+S S   SLFR  S+        V              + SIGG++       T    + +   S S  T     A+ L+            + + + +A    L + +Q   E+   + +   +N         +  + D +    L        + +S  +SC  S   + +   ++  +  ++ TE LRQ  E F  + ++WEKE+  Q+  +E+E   + E+ +  +K +++L  ++++++E C+ LQ+ +D++     +  GI M E+ L
Sbjct:   26 LLKKSEYLQQYPSWLGFVGTENKKQFAPTFLARNDTIWELIFSERGYVDMLLMVRDVYMLPFPDFEP-SQYDAASEGMTRAKLR-------------------EALFPGLDKLIKAHERILRPLLALHDQAEDKVIKHLGPCLAQLFDKTTQDSLARLYGGFMFAQRRIREQLVVCNRWPPVAAFFEKCKQDARSGRRSLGDCHTLIVQRWTKFEPLIDAIIRNTLNNPTEVEHLESVRKAVRCILKGAESVMTAREHAEKLNEFANGLLVPGGEDTEEHKLRAVMRSPSTTLINYGPLRIVLNPVGAQLSSPAVDVIGLGLNSCFCVLQKMPESG----RYQLFRG---TKMPPILWWGQVYGYFRRSVEK--GSFGFFILLQS-EAVLTLYRCSTVDDLVRWEKVINDGFSQWQTLQTLDHD----LTEAKSMIAARWQRTEAILQLLNELDENVLRAWSARNCVCLALIEEQLKILMSKNEADDGGNESDSRLTSSPSDSQKPPKAPGRSGRHTKAASPPTVSEVLEKNTFMKSLDNNSDLDQLFAMFQD--------TLARLHFSLVGRSPSSLSRSASDVEERKHTFSKPVRKNETFSARDNVNH---PGDSKDLARKSSSKKREANRLS-STMVSLFRTGSRDKPSADFFVSSSGQPPKGPSNQRVPSIGGSDASHTSSSTPLAPNMLPRPSFSFLTGTVGEALQLSVDGSVDGSSNSLSSQHSIEA-WTLLRNLDQLSEEFFPSVFRLRTKNDELDARVRCLETEKDKLET--LYKRAKQHGLIKSTCSSCGGSDGLDSSGADVSSALTVKQGTEKLRQDVENFRIKSEEWEKEYSRQRDALEREHTKLAEQWKRYEKAREELEHKQRQYEENCKTLQQQVDLHASRGVKFSGITMPEIRL 884          
BLAST of Rho guanine nucleotide exchange factor 28 vs. TrEMBL
Match: A0A075A423 (DH domain-containing protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_12415 PE=4 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 5.789e-54
Identity = 215/908 (23.68%), Postives = 405/908 (44.60%), Query Frame = 3
Query:  540 VISKGEYLMKY-QWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEI-NSDCKDSERVYEYMTTDNVILVFKGEVQFELRK-GGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSAS-----GRNSCTSTTTVTENDSKR-DQIDGYND------------EILD--TFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCS-SGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKS-------VKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFM--SSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAADDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNIN--QMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITXXXXXXXXXXXDLIEREKKHKEKCELLQRNMDMY----YQMHGINMEEVDL 3146
            ++ K EYL +Y  W  F+G+E  K++    + RND IWE++    G ++ L+++RDVY   +P  E  S     S    R++L                    + + PGLD LIK   +I   L+  H    D+ I  +G  L +  +      L++LYG F F ++   +++    ++P   ++  KC++D ++ +++L D    I   W +    ++ II  T+N+  EV  L   ++ ++ ++  AE++     +A+ L        +   +  +  ++   M + +  L+  G ++  L   G        D+ G+ L +C   LQ + E G    +YQLF+     K P +L WG +YGYFR    K   SF F+++++  E  +     ST  +   W+  +  GFS+WQ   ++  D    L E    I  +W  T+  ++L+  LD+++      R      ++EE+LK L +      G N   S  T + +DS++  +  G +             E+L+  TF+  L     ++    +F +         + RL FSL   S ++++RSAS++E RK    + V+K ETF++ D+  +   P D   L    + +K++  R+S S   SLFR  S+        V              + SIGG++       T    + +   S S  T     A+ L+            + + + +A    L + +Q   E+   + +   +N         +  + D +    L        + +S  +SC  S   + +   ++  +  ++ TE LRQ  E F  + ++WEKE+  Q+  +E+E   + E+ +  +K +++L  ++++++E C+ LQ+ +D++     +  GI M E+ L
Sbjct:   44 LLKKSEYLQQYPSWLGFVGTENKKQFAPTFLARNDTIWELIFSERGYVDMLLMVRDVYMLPFPDFEP-SQYDAASEGMTRAKLR-------------------EALFPGLDKLIKAHERILRPLLALHDQAEDKVIKHLGPCLAQLFDKTTQDSLARLYGGFMFAQRRIREQLVVCNRWPPVAAFFEKCKQDARSGRRSLGDCHTLIVQRWTKFEPLIDAIIRNTLNNPTEVEHLESVRKAVRCILKGAESVMTAREHAEKLNEFANGLLVPGGEDTEEHKLRAVMRSPSTTLINYGPLRIVLNPVGAQLSSPAVDVIGLGLNSCFCVLQKMPESG----RYQLFRG---TKMPPILWWGQVYGYFRRSVEK--GSFGFFILLQS-EAVLTLYRCSTVDDLVRWEKVINDGFSQWQTLQTLDHD----LTEAKSMIAARWQRTEAILQLLNELDENVLRAWSARNCVCLALIEEQLKILMSKNEADDGGNESDSRLTSSPSDSQKPPKAPGRSGRHTKAASPPTVSEVLEKNTFMKSLDNNSDLDQLFAMFQD--------TLARLHFSLVGRSPSSLSRSASDVEERKHTFSKPVRKNETFSARDNVNH---PGDSKDLARKSSSKKREANRLS-STMVSLFRTGSRDKPSADFFVSSSGQPPKGPSNQRVPSIGGSDASHTSSSTPLAPNMLPRPSFSFLTGTVGEALQLSVDGSVDGSSNSLSSQHSIEA-WTLLRNLDQLSEEFFPSVFRLRTKNDELDARVRCLETEKDKLET--LYKRAKQHGLIKSTCSSCGGSDGLDSSGADVSSALTVKQGTEKLRQDVENFRIKSEEWEKEYSRQRDALEREHTKLAEQWKRYEKAREELEHKQRQYEENCKTLQQQVDLHASRGVKFSGITMPEIRL 902          
BLAST of Rho guanine nucleotide exchange factor 28 vs. TrEMBL
Match: A0A5J4NSN7 (DH domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0011837 PE=4 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 1.119e-53
Identity = 164/622 (26.37%), Postives = 291/622 (46.78%), Query Frame = 3
Query:  513 VDEEEFIGSVISKGEYLMKY-QWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEIN-SDCKDSE--RVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAED-KLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGR----NSC------TSTTTVTENDSKRDQIDGY-------------NDEILD--TFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCSS-GANITRSASEIEARKRPSIQAVKKCETFTSHD 2285
            ++ E  +  +I   EYL KY  W  F G+E  KKY    I RND IWE++    G ++ L+++RDVY   +P  +      ++  + P   ++N++                + + PGLD LI+   ++   L+  H    D  +  +G  L +  +      LSKLYG F F ++   +++   K++P    +  KC++DP++ +++L D    I   W ++   L+ II+ T+N   EV  L   +  ++ L+  AE +     +   L    +N  +  S  +D+E  R+ + M + N  L+  G ++      G       ++ GVAL +C F LQ + E G     YQLF+     K P++L WG  YGYFR+   K   SF F++++   E  +     +T  E + W+  M  GF++W+    V  ++ D  L++ L     +W  T   + L+  LD  L+     R+     ++E+ L ++ A       NS        +   +T N   R +  GY               ++L   TF+ DL   +G +D     ++ V  +++  ++RL+F++  +    ++RSAS++E RKRP  +A++K ETF+  D
Sbjct:   93 MNPETSLHDLIRNSEYLQKYPSWLGFCGTENKKKYAPTFIARNDTIWELIFSERGYMDMLLMVRDVYMIPFPDFDP-----SQFDTGPDGGMTNAQLR--------------EALFPGLDQLIRAHERLLRPLLALHDKADDHVVKSLGSCLVKLFDRDTQLALSKLYGGFMFAQKRIRERLQICKRWPPVAQFFEKCKQDPRSARRSLEDCHMVIVQRWTKVETLLDAIIKNTLNHPVEVENLETARSTVRSLLKGAEAVMTAREHVDRLSDFADNLLVPVSSTEDTEEHRLRKEMKSPNTSLINYGPLRIVPYAIGQQAPAAVEVIGVALNSCFFVLQRLPESG----NYQLFKG---TKMPSILWWGQAYGYFRKSVEK--GSFGFFILLHS-ESVLTLFRCNTADELSRWEKIMNHGFAQWRETAPVTMESLDLDLSQTLSAAAQRWDRTNAVLNLLYDLDADLERAWAARSCVCLALIEDRLNQVKAKQNAMEDNSVDFDYLSITPPGLTGNFGSRPKSAGYIHRTPKAASTPTTTADLLSKYTFLTDL---DGKSD-----LDQVFALFQENLDRLRFAIMGAPPPGLSRSASDVEERKRPPSKAIRKTETFSVRD 677          
BLAST of Rho guanine nucleotide exchange factor 28 vs. Planmine SMEST
Match: SMESG000021797.1 (SMESG000021797.1)

HSP 1 Score: 2277.29 bits (5900), Expect = 0.000e+0
Identity = 1230/1245 (98.80%), Postives = 1232/1245 (98.96%), Query Frame = 3
Query:   87 MIXXXXXXXXXXPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRXXXXXXXXXXXXXXXRPTESDPTXXXXXXXXXXXXXXXXTLIDEPSFNNLVNQLSVPSXXXXXXXXXXXYYENNKMGSPKISTVENTDSVDEEEFIGSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCTSTTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAADDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITXXXXXXXXXXXDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKEXXXXXXXXXXXXXXXXXXXXXQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQTFFHQRLSIDIPYKSLLYRSLRWNKERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIXXXXXXXXXXXXXXXXXXYSNQQPNLGLPMELAA 3821
            MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSFNNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEF+GSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELK IEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSC STTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEW        VNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKA DDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKRSSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQTFFHQRLSIDIPYKSLLYRSLRWNKERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQ NL LP+ELAA
Sbjct:    1 MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSFNNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEFVGSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKNIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCASTTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEW--------VNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAVDDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKRSSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQTFFHQRLSIDIPYKSLLYRSLRWNKERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQANLNLPLELAA 1237          
BLAST of Rho guanine nucleotide exchange factor 28 vs. Planmine SMEST
Match: SMESG000021797.1 (SMESG000021797.1)

HSP 1 Score: 2232.22 bits (5783), Expect = 0.000e+0
Identity = 1213/1245 (97.43%), Postives = 1215/1245 (97.59%), Query Frame = 3
Query:   87 MIXXXXXXXXXXPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRXXXXXXXXXXXXXXXRPTESDPTXXXXXXXXXXXXXXXXTLIDEPSFNNLVNQLSVPSXXXXXXXXXXXYYENNKMGSPKISTVENTDSVDEEEFIGSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCTSTTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAADDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITXXXXXXXXXXXDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKEXXXXXXXXXXXXXXXXXXXXXQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQTFFHQRLSIDIPYKSLLYRSLRWNKERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIXXXXXXXXXXXXXXXXXXYSNQQPNLGLPMELAA 3821
            MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSFNNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEF+GSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELK IEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSC STTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKA DDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKRSSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQ                         ERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQ NL LP+ELAA
Sbjct:    1 MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSFNNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEFVGSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKNIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCASTTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAVDDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKRSSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQ-------------------------ERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQANLNLPLELAA 1220          
BLAST of Rho guanine nucleotide exchange factor 28 vs. Planmine SMEST
Match: SMESG000021797.1 (SMESG000021797.1)

HSP 1 Score: 2209.88 bits (5725), Expect = 0.000e+0
Identity = 1205/1245 (96.79%), Postives = 1207/1245 (96.95%), Query Frame = 3
Query:   87 MIXXXXXXXXXXPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRXXXXXXXXXXXXXXXRPTESDPTXXXXXXXXXXXXXXXXTLIDEPSFNNLVNQLSVPSXXXXXXXXXXXYYENNKMGSPKISTVENTDSVDEEEFIGSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKTIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCTSTTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAADDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITXXXXXXXXXXXDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKEXXXXXXXXXXXXXXXXXXXXXQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQTFFHQRLSIDIPYKSLLYRSLRWNKERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIXXXXXXXXXXXXXXXXXXYSNQQPNLGLPMELAA 3821
            MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSFNNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEF+GSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELK IEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSC STTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEW        VNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKA DDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKRSSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQ                         ERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQ NL LP+ELAA
Sbjct:    1 MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSEEDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSFNNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEFVGSVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNRLVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLFESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDKICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTLSDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAENIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQFELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDKFPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERNCWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKNIEMKWINTQHGIELIKSLDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCASTTTVTENDSKRDQIDGYNDEILDTFIPDLSTQEGINDWLDIFVEW--------VNRLKFSLCSSGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPPITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEKDAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAVDDAKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILVNSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANEKKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELLQRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKRSSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQ-------------------------ERQSLKTISSDNILDNRNKFSNSSYGAHNPKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVSTKSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQANLNLPLELAA 1212          
The following BLAST results are available for this feature:
BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RMX95.928e-5725.56Rho guanine nucleotide exchange factor 28 OS=Fasci... [more]
A0A504YTM84.360e-5525.26Rho guanine nucleotide exchange factor 28 OS=Fasci... [more]
A0A1S8X1N04.401e-5423.68DH domain-containing protein (Fragment) OS=Opistho... [more]
A0A075A4235.789e-5423.68DH domain-containing protein (Fragment) OS=Opistho... [more]
A0A5J4NSN71.119e-5326.37DH domain-containing protein OS=Paragonimus wester... [more]
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho guanine nucleotide exchange factor 28 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000021797.10.000e+098.80SMESG000021797.1[more]
SMESG000021797.10.000e+097.43SMESG000021797.1[more]
SMESG000021797.10.000e+096.79SMESG000021797.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007097 ID=SMED30007097|Name=Rho guanine nucleotide exchange factor 28|organism=Schmidtea mediterranea sexual|type=transcript|length=3968bp
ATATAAATCTTATATGAATAATCCGAATTTCAGTATTGTGAAAATATAAA
GATCAAAAACAAGGATTAAAATAATTTGCTCAAATTATGATTTCATCGTC
GGCAAGTGAAAAATCGTCCGAACCAACTCAGTTTAGAGCGGTTAGGAGAT
TTCCGGAAAATAACAGTGCATTGAATAACAATGTGTTGAAACCAGAACTT
ACTTACAATTCAAGCGTAAACAGACAGGATTCAGAGGAAGATAGGAAATC
GAAAAGTCTTCTATCGTCTTCAATAATGAAATCAAATCTCAGACCAACAG
AATCTGATCCTACGTCAAATATTTCAAACAGAAAAAGTCGGAGTTCCGAT
TCATCTTCAAATACTCTCATAGACGAACCGTCTTTCAATAACTTAGTGAA
TCAATTAAGTGTTCCATCAAAACTTCCACCAGCTAAATTACCAAAACCTA
AATATTATGAAAATAACAAAATGGGTTCACCAAAAATATCTACAGTAGAA
AATACGGATTCAGTAGATGAGGAAGAATTTATTGGTTCCGTTATCAGCAA
AGGCGAATACCTCATGAAATACCAATGGAATTCTTTTATTGGTTCCGAAG
AATTGAAGAAATACCCTAGAAAAGTTATCGAAAGAAATGATAATATATGG
GAAATGGTTTCATTACACTATGGATGTCTTAACAGATTAGTGATCCTTCG
TGATGTTTATGGAAAATCATATCCATATCTTGAAGCCGGGTCTAATCAAA
TCACTCGGAGTTCTTCTACTCCAAGAAGTGAGTTGTCAAACTCTGAAAGA
CATGGATCCATTTCTGATAAATCAAATAATTTATTTGAATCAATCGACAC
AATAGTTCCTGGCCTGGACCCTTTAATCAAATGCTTTGCTAAAATAACTA
GTAATCTTGTAAATAATCACCTAATCAGACCTGATCGCGCCATTACTGAT
ATTGGAAAAGTACTTAATGAGTCTATAAATGATAAAATTTGCTCAGATTT
GTCCAAGCTTTATGGACACTTTAATTTCCAGCGACAAATCGCTTCTCAAA
AAATCGCTCAATTCAAAAAATATCCCACATTTCTAAGTTATATTCATAAA
TGTGAAAGGGATCCAAAAACAGAAAAGAAAACCTTGTCTGACTATATACA
AGATATAAGCATGTTTTGGGCTAGGATGCTCATACCGTTAAATGTCATTA
TTGAAAAAACGATGAATGACTCTCAAGAAGTCAATTTTCTCAATAAATAC
AAAGAAAAAATTCAAAAACTCATAACCGATGCCGAAAATATTCAAGGCAA
ATTTGTGAATGCCCAAGTGTTGAAAACAATTGTAGAAAATTATGAGATAA
ACTCAGATTGTAAGGATAGTGAAAGAGTATATGAATATATGACAACGGAT
AATGTCATATTGGTTTTCAAAGGGGAAGTTCAATTTGAATTGAGAAAAGG
AGGTACAGGAAGAGTAGTCTATAGAGATTTAACAGGAGTAGCTTTAGAAA
ATTGTTTATTCTTTTTACAAACGATTTCTGAACCAGGAAAAAACGTTTGT
AAATATCAATTATTTCAAGACAGAGAAAGGGATAAATTTCCGGCTTTACT
TGAATGGGGACATTTATATGGATACTTCAGAGAAGAAGACTCTAAAAATA
CAAATTCATTCAGTTTCTATGTAATTATTGAAAAACCAGAACCATCAATG
AGACGTTTATTTGTTAGTACAACAACTGAACGGAATTGCTGGAAAAATTG
CATGATGCAAGGATTTTCGCGTTGGCAAAACAAAAATTCAGTGATCAAAG
ATGCAGAAGACAAACTGAATGAAGAACTAAAAACTATTGAAATGAAATGG
ATCAACACTCAGCATGGAATAGAATTAATTAAATCTCTTGATCAATCATT
AAAAGTTCAATTGGAATATCGGACTGTCGGTTTTCGTTGTATTCTGGAAG
AAGAGTTGAAGCGGTTGAGTGCATCTGGAAGGAATAGTTGTACTTCAACA
ACAACTGTTACTGAGAACGATTCGAAAAGAGATCAAATTGATGGCTACAA
TGACGAGATTTTAGATACCTTTATACCAGATTTATCAACCCAAGAAGGAA
TTAATGACTGGCTTGATATTTTCGTGGAATGGGTAATTGTAGTTTATAAA
CTAATAGTTAATAGACTGAAGTTCAGCCTATGCAGTAGTGGTGCAAACAT
TACGAGAAGCGCGTCTGAGATCGAAGCTCGAAAAAGACCATCAATCCAAG
CAGTGAAAAAATGTGAAACTTTCACAAGTCATGATTCACCAACTTACGAA
AAACGTCCAACGGATGTTGCCTTTCTTAATCCACCAATAACGAAACAAAA
GAAAGATCAGAGACGTATCAGTGTGAGTGGGTTTACGAGTTTATTTCGTA
TTAACAGTAAAAGTGTGAAACATGATGACTCTGATAAAACGAATGATCAT
TTAAGCACAATTGATTCTATTGGGGGTAACGAAAAAGACGCGGCTTATTT
CGAAACGGACGAAACTGAATCATTCATGTCTAGTTTATCAAAAGATACTG
TAGCTGCGTTCAATGCTATAAATTTAGCTGCAAAAAATGTAACGATGGAA
TTGATAGGATTGCGAACACAAAAAGCGGCTGATGATGCGAAAATTAAAAA
ACTTAGTGACGAGAATCAATCTTATAGAGAGTACGTAAAGAAAATACAAA
AGTGCTCTGCAGAAAACCAATCTTCATATCCAACAACCACTTCTGTTTAC
CAAAAGGTTGATTCTATCCATAATAACATTTTAGTGAATTCACCACCAAA
TGATATTATGACAGAATCCATGGAAAATTCATGCGAAAATTCAGGAAATT
ATAATATAAATCAAATGAATCAAATGGTAGAGTGTCGCAGACAGACCGAA
AATCTCCGACAGATGTATGAGCAATTTGCAAACGAGAAAAAGAAATGGGA
AAAAGAACATGAAACACAAAAGTTGAAAATCGAAAAAGAAAAGGATTCAA
TTACAGAAAAACAACAGGAATTGAAAAAAGAGAAACAAGACCTCATTGAA
CGAGAAAAGAAACACAAAGAGAAATGTGAGTTATTGCAAAGAAACATGGA
CATGTATTACCAAATGCATGGCATAAATATGGAAGAAGTCGATTTGCAAT
CTGATAAATCATCTAACAAAGTAAAGGAGTCTGTTCAATCATCTCCCCAT
TCTCGTTCACATAGTGACGATGCCAGTCACAAGCGCAGCTCACAAAGTCC
AATTTCACCACCACTTGCGTCAACAGAAAACGCAGCATATTCTGTAGATT
CCAGATCTTATCCAGCCGATTTCAGTCAACCAAACAAAATTCCAGATCAA
TTGCGAGGAAGCCTAATTAATCAGACTTTTTTTCACCAGAGATTATCGAT
TGACATCCCTTATAAGAGTTTACTGTACCGTAGCCTTCGGTGGAATAAGG
AGCGGCAATCTTTAAAGACTATCAGTTCGGATAATATATTGGATAATCGG
AATAAATTTTCGAATAGCAGTTACGGGGCACATAATCCAAAAATGACTCC
TTCGATAGATCGGAAGGAAAGATTTTCCAGTAATAACATGTGTTTCAGTA
TTGATGATGCTGAAGAGAAAAATTGGTCCAGTGATGTTGACGATGTGACT
TTTCAACAGAGAAAATCAGGGTCAAATGTGTCTACTAAATCCATATCACA
AACTGATCATAGCCTTATTAGGAATACATCTAATACCGTCACGTCAACAA
ATTCCAATCGCGGTGTTACCAACTACTCCAATCAGCAGCCAAATCTCGGT
TTACCTATGGAACTCGCTGCCAAATCCAAAATAAAAAAAGATAAAAGTCG
AAAGATTTTAAAACTGAAAACTAAAAATTGATATTGCTTAAATAAATAAA
TTTATATTTGAAATTAAGCACTTTCTACAAATTGTTGCTGGTGTTATGAA
ATAATACTGTTTTTTCAG
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protein sequence of SMED30007097-orf-1

>SMED30007097-orf-1 ID=SMED30007097-orf-1|Name=SMED30007097-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1265bp
MISSSASEKSSEPTQFRAVRRFPENNSALNNNVLKPELTYNSSVNRQDSE
EDRKSKSLLSSSIMKSNLRPTESDPTSNISNRKSRSSDSSSNTLIDEPSF
NNLVNQLSVPSKLPPAKLPKPKYYENNKMGSPKISTVENTDSVDEEEFIG
SVISKGEYLMKYQWNSFIGSEELKKYPRKVIERNDNIWEMVSLHYGCLNR
LVILRDVYGKSYPYLEAGSNQITRSSSTPRSELSNSERHGSISDKSNNLF
ESIDTIVPGLDPLIKCFAKITSNLVNNHLIRPDRAITDIGKVLNESINDK
ICSDLSKLYGHFNFQRQIASQKIAQFKKYPTFLSYIHKCERDPKTEKKTL
SDYIQDISMFWARMLIPLNVIIEKTMNDSQEVNFLNKYKEKIQKLITDAE
NIQGKFVNAQVLKTIVENYEINSDCKDSERVYEYMTTDNVILVFKGEVQF
ELRKGGTGRVVYRDLTGVALENCLFFLQTISEPGKNVCKYQLFQDRERDK
FPALLEWGHLYGYFREEDSKNTNSFSFYVIIEKPEPSMRRLFVSTTTERN
CWKNCMMQGFSRWQNKNSVIKDAEDKLNEELKTIEMKWINTQHGIELIKS
LDQSLKVQLEYRTVGFRCILEEELKRLSASGRNSCTSTTTVTENDSKRDQ
IDGYNDEILDTFIPDLSTQEGINDWLDIFVEWVIVVYKLIVNRLKFSLCS
SGANITRSASEIEARKRPSIQAVKKCETFTSHDSPTYEKRPTDVAFLNPP
ITKQKKDQRRISVSGFTSLFRINSKSVKHDDSDKTNDHLSTIDSIGGNEK
DAAYFETDETESFMSSLSKDTVAAFNAINLAAKNVTMELIGLRTQKAADD
AKIKKLSDENQSYREYVKKIQKCSAENQSSYPTTTSVYQKVDSIHNNILV
NSPPNDIMTESMENSCENSGNYNINQMNQMVECRRQTENLRQMYEQFANE
KKKWEKEHETQKLKIEKEKDSITEKQQELKKEKQDLIEREKKHKEKCELL
QRNMDMYYQMHGINMEEVDLQSDKSSNKVKESVQSSPHSRSHSDDASHKR
SSQSPISPPLASTENAAYSVDSRSYPADFSQPNKIPDQLRGSLINQTFFH
QRLSIDIPYKSLLYRSLRWNKERQSLKTISSDNILDNRNKFSNSSYGAHN
PKMTPSIDRKERFSSNNMCFSIDDAEEKNWSSDVDDVTFQQRKSGSNVST
KSISQTDHSLIRNTSNTVTSTNSNRGVTNYSNQQPNLGLPMELAAKSKIK
KDKSRKILKLKTKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0005085guanyl-nucleotide exchange factor activity
GO:0005089Rho guanyl-nucleotide exchange factor activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR035899DBL_dom_sf
IPR000219DH-domain
Vocabulary: biological process
TermDefinition
GO:0035023regulation of Rho protein signal transduction
GO:0043547positive regulation of GTPase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 930..1003
NoneNo IPR availableCOILSCoilCoilcoord: 385..405
NoneNo IPR availableCOILSCoilCoilcoord: 567..587
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1021..1061
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1035..1049
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 221..240
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..45
IPR035899Dbl homology (DH) domain superfamilyGENE3DG3DSA:1.20.900.10coord: 157..419
e-value: 6.5E-13
score: 50.6
IPR035899Dbl homology (DH) domain superfamilySUPERFAMILYSSF48065DBL homology domain (DH-domain)coord: 164..418