U2 snRNP auxiliary factor large subunit

Overview


Name	U2 snRNP auxiliary factor large subunit
Smed ID	SMED30007069
Length (bp)	3712

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of U2 snRNP auxiliary factor large subunit (SMED30007069) t-SNE clustered cells

Violin plots show distribution of expression levels for U2 snRNP auxiliary factor large subunit (SMED30007069) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of U2 snRNP auxiliary factor large subunit (SMED30007069) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for U2 snRNP auxiliary factor large subunit (SMED30007069) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30007069

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X2 cell	SMED30007069	SMESG000078029.1	SmedASXL_005625	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 152.525 bits (384), Expect = 4.239e-40
Identity = 100/184 (54.35%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  126 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 307

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 152.525 bits (384), Expect = 1.747e-38
Identity = 100/184 (54.35%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  290 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 471

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: U2AF2 (U2 small nuclear RNA auxiliary factor 2 [Source:HGNC Symbol;Acc:HGNC:23156])

HSP 1 Score: 147.902 bits (372), Expect = 5.923e-37
Identity = 102/190 (53.68%), Postives = 132/190 (69.47%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKN------PXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN      P+T   T + +     +     MG  G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  290 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMG--GHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042])

HSP 1 Score: 70.4774 bits (171), Expect = 1.040e-11
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ P+++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SET KA++AL GR F+GR VV   YD ER+ N
Sbjct:  413 QESTVMVLRNMVDPKDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDN 508

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042])

HSP 1 Score: 70.4774 bits (171), Expect = 1.046e-11
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ P+++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SET KA++AL GR F+GR VV   YD ER+ N
Sbjct:  405 QESTVMVLRNMVDPKDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDN 500

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 132.109 bits (331), Expect = 3.761e-32
Identity = 91/179 (50.84%), Postives = 119/179 (66.48%), Query Frame = 3

Query: 1704 SSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
             S G SKGYAF EY D ++TD+A  GL GMQLG+K++VVQ A    +       T L  +     G+D S G  G+ TEILCL NM+T +EL  D+EYEEI+EDV++ECSKYG V SL+IPRP   E+    GVGK++VEF++ S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:  303 DSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQR----HNTNLPNSASAIAGIDLSQG-AGRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY--EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 474

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 132.109 bits (331), Expect = 3.842e-32
Identity = 91/179 (50.84%), Postives = 119/179 (66.48%), Query Frame = 3

Query: 1704 SSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
             S G SKGYAF EY D ++TD+A  GL GMQLG+K++VVQ A    +       T L  +     G+D S G  G+ TEILCL NM+T +EL  D+EYEEI+EDV++ECSKYG V SL+IPRP   E+    GVGK++VEF++ S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:  300 DSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQR----HNTNLPNSASAIAGIDLSQG-AGRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY--EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 471

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 132.109 bits (331), Expect = 5.096e-32
Identity = 91/179 (50.84%), Postives = 119/179 (66.48%), Query Frame = 3

Query: 1704 SSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
             S G SKGYAF EY D ++TD+A  GL GMQLG+K++VVQ A    +       T L  +     G+D S G  G+ TEILCL NM+T +EL  D+EYEEI+EDV++ECSKYG V SL+IPRP   E+    GVGK++VEF++ S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:  325 DSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQR----HNTNLPNSASAIAGIDLSQG-AGRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY--EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 496

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 100.523 bits (249), Expect = 2.083e-24
Identity = 74/150 (49.33%), Postives = 99/150 (66.00%), Query Frame = 3

Query: 1791 MQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            MQLG+K++VVQ A    +       T L  +     G+D S G  G+ TEILCL NM+T +EL  D+EYEEI+EDV++ECSKYG V SL+IPRP   E+    GVGK++VEF++ S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:    1 MQLGDKQLVVQLACANQQR----HNTNLPNSASAIAGIDLSQGA-GRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY--EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 143

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Match: uaf-1 (Splicing factor U2AF 65 kDa subunit [Source:UniProtKB/Swiss-Prot;Acc:P90978])

HSP 1 Score: 100.523 bits (249), Expect = 2.083e-24
Identity = 74/150 (49.33%), Postives = 99/150 (66.00%), Query Frame = 3

Query: 1791 MQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            MQLG+K++VVQ A    +       T L  +     G+D S G  G+ TEILCL NM+T +EL  D+EYEEI+EDV++ECSKYG V SL+IPRP   E+    GVGK++VEF++ S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:    1 MQLGDKQLVVQLACANQQR----HNTNLPNSASAIAGIDLSQGA-GRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY--EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 143

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307177)

HSP 1 Score: 154.451 bits (389), Expect = 1.789e-40
Identity = 96/183 (52.46%), Postives = 132/183 (72.13%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEY D S+TD++  GL GMQLG+K+++VQRAS+GAKN    A         Q  G+  ++ T G  TE+LCL NM+TP+EL D+EEYE+I+ED+KEEC+KYG V S++IPRP    E    G GK++VEF++  +  KA +AL GR FS RVVVTS++DP++Y    F
Sbjct:  182 LVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKN-AQNAANTTQSVMLQVPGLS-NVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVP--GCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 360

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307178)

HSP 1 Score: 154.066 bits (388), Expect = 7.483e-40
Identity = 96/183 (52.46%), Postives = 132/183 (72.13%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEY D S+TD++  GL GMQLG+K+++VQRAS+GAKN    A         Q  G+  ++ T G  TE+LCL NM+TP+EL D+EEYE+I+ED+KEEC+KYG V S++IPRP    E    G GK++VEF++  +  KA +AL GR FS RVVVTS++DP++Y    F
Sbjct:  238 LVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKN-AQNAANTTQSVMLQVPGLS-NVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVP--GCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0074234)

HSP 1 Score: 154.066 bits (388), Expect = 7.483e-40
Identity = 96/183 (52.46%), Postives = 132/183 (72.13%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEY D S+TD++  GL GMQLG+K+++VQRAS+GAKN    A         Q  G+  ++ T G  TE+LCL NM+TP+EL D+EEYE+I+ED+KEEC+KYG V S++IPRP    E    G GK++VEF++  +  KA +AL GR FS RVVVTS++DP++Y    F
Sbjct:  238 LVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKN-AQNAANTTQSVMLQVPGLS-NVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVP--GCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: U2af50 (gene:FBgn0005411 transcript:FBtr0307176)

HSP 1 Score: 154.451 bits (389), Expect = 8.506e-40
Identity = 96/183 (52.46%), Postives = 132/183 (72.13%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEY D S+TD++  GL GMQLG+K+++VQRAS+GAKN    A         Q  G+  ++ T G  TE+LCL NM+TP+EL D+EEYE+I+ED+KEEC+KYG V S++IPRP    E    G GK++VEF++  +  KA +AL GR FS RVVVTS++DP++Y    F
Sbjct:  249 LVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKN-AQNAANTTQSVMLQVPGLS-NVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVP--GCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 427

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Match: LS2 (gene:FBgn0034834 transcript:FBtr0072052)

HSP 1 Score: 141.739 bits (356), Expect = 2.185e-35
Identity = 87/178 (48.88%), Postives = 121/178 (67.98%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY 2225
            ++ D++T L+KG+AF EY D SVTD A  GL GM LG++R+VVQR+  G KN        +L         DP     G  TE LCL NM+ PEEL DDEE+E+I  D+K+EC+K+G V S++IPRP G  +  + G GK++V+F +  ++ KA+KAL+GR FSGR+V+TS+YDPE+Y
Sbjct:  273 LVMDTNTNLNKGFAFFEYCDPSVTDHAIAGLHGMLLGDRRLVVQRSIPGGKNAFPGHLPTVLQVPGISTLQDP-----GSPTETLCLLNMVRPEELLDDEEFEDIRTDIKQECAKFGEVRSIKIPRPIG--QFPKRGCGKVFVQFESVEDSQKALKALSGRKFSGRIVMTSYYDPEKY 443

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881])

HSP 1 Score: 147.517 bits (371), Expect = 5.165e-37
Identity = 100/186 (53.76%), Postives = 130/186 (69.89%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGM--DPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +V D+A  GL GMQL +K+++VQRAS+GAKN  T  +        Q  G+  +P +   G  TE+LCL NM+ PEEL DDEEYEEIVEDVKEECSKYG V S++IPRP    +    G GKI+VEF++  ++ KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  298 LVKDSATGLSKGYAFCEYVDVNVNDQAIAGLNGMQLADKKLLVQRASVGAKN-ATMTSINETPVTLQVPGLTSNPMIQMGGIPTEVLCLMNMVAPEELIDDEEYEEIVEDVKEECSKYGQVKSIEIPRPVDGLDIP--GTGKIFVEFTSVYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 480

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881])

HSP 1 Score: 147.517 bits (371), Expect = 5.365e-37
Identity = 100/186 (53.76%), Postives = 130/186 (69.89%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGM--DPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +V D+A  GL GMQL +K+++VQRAS+GAKN  T  +        Q  G+  +P +   G  TE+LCL NM+ PEEL DDEEYEEIVEDVKEECSKYG V S++IPRP    +    G GKI+VEF++  ++ KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  293 LVKDSATGLSKGYAFCEYVDVNVNDQAIAGLNGMQLADKKLLVQRASVGAKN-ATMTSINETPVTLQVPGLTSNPMIQMGGIPTEVLCLMNMVAPEELIDDEEYEEIVEDVKEECSKYGQVKSIEIPRPVDGLDIP--GTGKIFVEFTSVYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 475

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131])

HSP 1 Score: 146.747 bits (369), Expect = 8.453e-37
Identity = 97/185 (52.43%), Postives = 130/185 (70.27%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQ-ETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +++D+A  GL GMQLG+K+++VQRAS+G+KN T        +  Q    M+ S+   G   TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    +    G GKI+VEF +  ++ KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  283 LVKDSATGLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTTLTGINQTPVTLQVPGLMNSSVNQMGGIPTEVLCLMNMVAPEELLDDEEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLDIP--GTGKIFVEFMSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 465

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131])

HSP 1 Score: 146.747 bits (369), Expect = 8.782e-37
Identity = 97/185 (52.43%), Postives = 130/185 (70.27%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQ-ETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +++D+A  GL GMQLG+K+++VQRAS+G+KN T        +  Q    M+ S+   G   TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    +    G GKI+VEF +  ++ KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  288 LVKDSATGLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTTLTGINQTPVTLQVPGLMNSSVNQMGGIPTEVLCLMNMVAPEELLDDEEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLDIP--GTGKIFVEFMSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 470

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Match: u2af2b (U2 small nuclear RNA auxiliary factor 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1881])

HSP 1 Score: 107.842 bits (268), Expect = 1.728e-25
Identity = 76/130 (58.46%), Postives = 95/130 (73.08%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGM--DPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPT 2075
            +++DS+TGLSKGYAFCEY D +V D+A  GL GMQL +K+++VQRAS+GAKN  T  +        Q  G+  +P +   G  TE+LCL NM+ PEEL DDEEYEEIVEDVKEECSKYG V S++IPRP 
Sbjct:   74 LVKDSATGLSKGYAFCEYVDVNVNDQAIAGLNGMQLADKKLLVQRASVGAKN-ATMTSINETPVTLQVPGLTSNPMIQMGGIPTEVLCLMNMVAPEELIDDEEYEEIVEDVKEECSKYGQVKSIEIPRPV 202

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616])

HSP 1 Score: 150.984 bits (380), Expect = 1.627e-38
Identity = 98/184 (53.26%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DD+EYEEIVEDV++EC KYG+V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  238 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDDEYEEIVEDVRDECGKYGAVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDGYHRRDFW 419

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616])

HSP 1 Score: 151.369 bits (381), Expect = 2.941e-38
Identity = 98/184 (53.26%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DD+EYEEIVEDV++EC KYG+V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  275 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDDEYEEIVEDVRDECGKYGAVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDGYHRRDFW 456

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: mbp (myelin basic protein [Source:Xenbase;Acc:XB-GENE-959616])

HSP 1 Score: 150.984 bits (380), Expect = 3.957e-38
Identity = 98/184 (53.26%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DD+EYEEIVEDV++EC KYG+V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  288 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDDEYEEIVEDVRDECGKYGAVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDGYHRRDFW 469

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: puf60 (poly(U) binding splicing factor 60KDa [Source:NCBI gene;Acc:100145091])

HSP 1 Score: 70.8626 bits (172), Expect = 6.581e-12
Identity = 44/101 (43.56%), Postives = 61/101 (60.40%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ P ++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SET KA++AL GR F+GR VV   YD ER+ N
Sbjct:  361 QESTVMVLRNMVDPRDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMSSETQKAIQALNGRWFAGRKVVAEVYDQERFDN 456

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Match: puf60 (poly(U) binding splicing factor 60KDa [Source:NCBI gene;Acc:100145091])

HSP 1 Score: 70.4774 bits (171), Expect = 1.213e-11
Identity = 44/101 (43.56%), Postives = 61/101 (60.40%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ P ++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SET KA++AL GR F+GR VV   YD ER+ N
Sbjct:  420 QESTVMVLRNMVDPRDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMSSETQKAIQALNGRWFAGRKVVAEVYDQERFDN 515

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 152.525 bits (384), Expect = 2.910e-40
Identity = 100/184 (54.35%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  126 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 307

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 152.525 bits (384), Expect = 1.199e-38
Identity = 100/184 (54.35%), Postives = 129/184 (70.11%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN T +      +  Q    M   +   G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  290 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 471

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: U2af2 (U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 [Source:MGI Symbol;Acc:MGI:98886])

HSP 1 Score: 147.902 bits (372), Expect = 3.705e-37
Identity = 102/190 (53.68%), Postives = 132/190 (69.47%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKN------PXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN      P+T   T + +     +     MG  G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  290 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMG--GHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: Puf60 (poly-U binding splicing factor 60 [Source:MGI Symbol;Acc:MGI:1915209])

HSP 1 Score: 70.0922 bits (170), Expect = 1.190e-11
Identity = 44/101 (43.56%), Postives = 61/101 (60.40%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ P+++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SET KA++AL GR F GR VV   YD ER+ N
Sbjct:  447 QESTVMVLRNMVDPKDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDN 542

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Match: Puf60 (poly-U binding splicing factor 60 [Source:MGI Symbol;Acc:MGI:1915209])

HSP 1 Score: 70.0922 bits (170), Expect = 1.306e-11
Identity = 44/101 (43.56%), Postives = 61/101 (60.40%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ P+++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SET KA++AL GR F GR VV   YD ER+ N
Sbjct:  464 QESTVMVLRNMVDPKDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDN 559

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|Q24562|U2AF2_DROME (Splicing factor U2AF 50 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af50 PE=1 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 7.501e-39
Identity = 96/183 (52.46%), Postives = 132/183 (72.13%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEY D S+TD++  GL GMQLG+K+++VQRAS+GAKN    A         Q  G+  ++ T G  TE+LCL NM+TP+EL D+EEYE+I+ED+KEEC+KYG V S++IPRP    E    G GK++VEF++  +  KA +AL GR FS RVVVTS++DP++Y    F
Sbjct:  238 LVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKN-AQNAANTTQSVMLQVPGLS-NVVTSGPPTEVLCLLNMVTPDELRDEEEYEDILEDIKEECTKYGVVRSVEIPRPIEGVEVP--GCGKVFVEFNSVLDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF 416

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P26369|U2AF2_MOUSE (Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3)

HSP 1 Score: 147.902 bits (372), Expect = 2.592e-36
Identity = 102/190 (53.68%), Postives = 132/190 (69.47%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKN------PXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN      P+T   T + +     +     MG  G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  290 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSLPSTINQTPVTLQVPGLMSSQVQMG--GHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P26368|U2AF2_HUMAN (Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4)

HSP 1 Score: 147.902 bits (372), Expect = 2.844e-36
Identity = 102/190 (53.68%), Postives = 132/190 (69.47%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKN------PXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +VTD+A  GL GMQLG+K+++VQRAS+GAKN      P+T   T + +     +     MG  G  TE+LCL NM+ PEEL DDEEYEEIVEDV++ECSKYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  290 LVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMG--GHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVP--GCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 475

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P90978|U2AF2_CAEEL (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 PE=2 SV=2)

HSP 1 Score: 132.109 bits (331), Expect = 6.483e-31
Identity = 91/179 (50.84%), Postives = 119/179 (66.48%), Query Frame = 3

Query: 1704 SSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
             S G SKGYAF EY D ++TD+A  GL GMQLG+K++VVQ A    +       T L  +     G+D S G  G+ TEILCL NM+T +EL  D+EYEEI+EDV++ECSKYG V SL+IPRP   E+    GVGK++VEF++ S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:  325 DSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQQR----HNTNLPNSASAIAGIDLSQG-AGRATEILCLMNMVTEDELKADDEYEEILEDVRDECSKYGIVRSLEIPRPY--EDHPVPGVGKVFVEFASTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 496

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Match: sp|P90727|U2AF2_CAEBR (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis briggsae OX=6238 GN=uaf-1 PE=3 SV=2)

HSP 1 Score: 129.798 bits (325), Expect = 3.931e-30
Identity = 92/179 (51.40%), Postives = 118/179 (65.92%), Query Frame = 3

Query: 1704 SSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
             S G SKGYAF EY D ++TD+A  GL GMQLG+K++VVQ A        T   T L  +     G+D S G  G+ TEILCL NM+T +EL  DE+YEEI+EDV+EECSKYG V SL+IPRP   ++    GVGK++VEF+  S+  +A  AL GR F+ R VVTS+YD ++Y N  F
Sbjct:  316 DSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACAN----QTRHNTHLPNSASAIAGIDLSQG-AGRATEILCLMNMVTEDELRSDEDYEEILEDVREECSKYGIVRSLEIPRPY--DDHPVPGVGKVFVEFATTSDCQRAQAALTGRKFANRTVVTSYYDVDKYHNRQF 487

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A0B7A573 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF97036 PE=4 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 7.043e-43
Identity = 106/183 (57.92%), Postives = 133/183 (72.68%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGL+KG+AFCEYAD SVTD+AC GL GMQLG+K+++VQRAS+GAKN            + Q  G++ + G  G  TE+LCL NMITPEEL D++EYE+IVEDVKEEC +YG V SL+IPRP    E    GVGKI+VEF++  E  KA  AL GR FS RVVVTS+YDP+RY    F
Sbjct:   19 LVKDSATGLTKGFAFCEYADVSVTDQACAGLNGMQLGDKKLIVQRASVGAKN-----AQQQTPVQLQVPGLNLNQGA-GPATEVLCLMNMITPEELIDEDEYEDIVEDVKEECGRYGIVMSLEIPRPIKGVEVP--GVGKIFVEFNSIIECQKAQAALTGRKFSNRVVVTSYYDPDRYHRREF 193

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A1V9XTQ2 (Splicing factor U2AF 50 kDa subunit-like (Fragment) OS=Tropilaelaps mercedesae OX=418985 GN=BIW11_07505 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.375e-41
Identity = 97/184 (52.72%), Postives = 138/184 (75.00%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSM-GTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEYAD ++TD+A  GL GMQLGEK+++VQRAS+GAKN     ++     + Q  GM   + G+ G  TE+LCL N++TP+EL D+EEYE+I+ED++EECSKYG V S++IPRP    +   NGVGK++VEF++ ++  KA +AL GR FS RVVVTS+++P++Y    F
Sbjct:   38 LVKDTATGLSKGYAFCEYADAAITDEAIAGLNGMQLGEKKLIVQRASVGAKN-----SSMAAPVQIQVPGMASGVPGSSGPATEVLCLMNLVTPDELRDEEEYEDILEDIQEECSKYGHVKSIEIPRPIDGVD--VNGVGKVFVEFNSIADCQKAQQALTGRKFSNRVVVTSYFEPDKYHRRQF 214

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A2C9JG43 (U2 snRNP auxiliary factor large subunit OS=Biomphalaria glabrata OX=6526 GN=106069459 PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.635e-40
Identity = 108/183 (59.02%), Postives = 133/183 (72.68%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGL+KG+AFCEYAD SVTD+AC GL GMQLG+K+++VQRAS+GAKN            + Q  G++ + G  G  TE+LCL NMITPEEL D+EEYE+IVEDVKEEC KYG V SL+IPRP    E    GVGKI+VEF++  E  KA  AL GR FS RVVVTS+YDP+RY    F
Sbjct:  267 LVKDSATGLTKGFAFCEYADVSVTDQACAGLNGMQLGDKKLIVQRASVGAKN-----AQQQAPVQLQVPGLNLNQG-AGPATEVLCLMNMITPEELVDEEEYEDIVEDVKEECGKYGIVMSLEIPRPIKGVEVP--GVGKIFVEFNSIIECQKAQAALTGRKFSNRVVVTSYYDPDRYHRREF 441

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A1D2N903 (U2 snRNP auxiliary factor large subunit OS=Orchesella cincta OX=48709 GN=Ocin01_04946 PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.968e-40
Identity = 102/183 (55.74%), Postives = 135/183 (73.77%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGLSKGYAFCEYAD S+TD A  GL GMQLG+K+++VQRAS+GAKN   A   A+ I          SMGT G  TE+LCL NM+TP+EL D+EEY++I+ED+KEEC KYG V S++IPRP   E     G GK++VEF+++++  KA +ALAGR FS RVVVTS++DP++Y    F
Sbjct:  268 LVKDSATGLSKGYAFCEYADISITDHAIAGLNGMQLGDKKLIVQRASIGAKNSANAGLGAVAIQVPGMPMNMVSMGTSGPATEVLCLMNMVTPDELKDEEEYDDILEDIKEECGKYGIVRSIEIPRPI--EGVDVPGCGKVFVEFNSQADCQKAQQALAGRRFSNRVVVTSYFDPDKYHRREF 448

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Match: A0A2S2P6Q0 (Splicing factor U2AF subunit OS=Schizaphis graminum OX=13262 GN=U2af50_5 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 3.140e-40
Identity = 96/183 (52.46%), Postives = 130/183 (71.04%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++D++TGLSKGYAFCEYAD  +TD+A  GL GMQLGEK+++VQRAS+GAKNP        +      +     +GT G  TE+LCL NM+TP+EL D+EEYE+I+ED++EEC+KYG V SL+IPRP   E     G GK+++EF+   +  KA +ALAGR F+ RVVVTSF +P++Y    F
Sbjct:   32 LVKDAATGLSKGYAFCEYADVVMTDQAIAGLNGMQLGEKKLIVQRASIGAKNPGLGQVPVTIQVPGLTV-----VGTAGPPTEVLCLLNMVTPDELKDEEEYEDILEDIREECNKYGVVRSLEIPRPI--EGIDVPGCGKVFIEFNTIVDCQKAQQALAGRKFNNRVVVTSFMEPDKYHRREF 207

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2b (splicing factor U2AF 65 kDa subunit [Source:NCBI gene;Acc:103026862])

HSP 1 Score: 150.214 bits (378), Expect = 4.494e-38
Identity = 97/184 (52.72%), Postives = 128/184 (69.57%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQ-ETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGLSKGYAFCEY D ++ D+A  GL GMQLG+K+++VQRAS+GAKN T  +     +  Q    M+ SM   G   TE+LCL NM+ PEEL DD+EYEEIVEDV++ECSKYG V S++IPRP    +    G GKI+VEF+   ++ KAM+ L GR F+ RVVVT + DP+ Y    F
Sbjct:  274 LVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGAKNATMTSINETPVTLQVPGLMNTSMNQMGGIPTEVLCLMNMVAPEELLDDDEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLDIP--GTGKIFVEFTTLYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDF 455

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2b (splicing factor U2AF 65 kDa subunit [Source:NCBI gene;Acc:103026862])

HSP 1 Score: 149.828 bits (377), Expect = 6.797e-38
Identity = 97/184 (52.72%), Postives = 128/184 (69.57%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQ-ETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGLSKGYAFCEY D ++ D+A  GL GMQLG+K+++VQRAS+GAKN T  +     +  Q    M+ SM   G   TE+LCL NM+ PEEL DD+EYEEIVEDV++ECSKYG V S++IPRP    +    G GKI+VEF+   ++ KAM+ L GR F+ RVVVT + DP+ Y    F
Sbjct:  281 LVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGAKNATMTSINETPVTLQVPGLMNTSMNQMGGIPTEVLCLMNMVAPEELLDDDEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLDIP--GTGKIFVEFTTLYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDF 462

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: u2af2a (splicing factor U2AF 65 kDa subunit-like [Source:NCBI gene;Acc:103024792])

HSP 1 Score: 145.976 bits (367), Expect = 1.230e-36
Identity = 99/187 (52.94%), Postives = 132/187 (70.59%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXG--MDPSMGTCGQ-ETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D +++D+A  GL GMQLG+K+++VQRAS+G+KN +   T         Q+   M+ SM   G   TE+LCL NM++PEEL DD+EYEEIVEDV+EECSKYG V S++IPRP    E    G GKI+VEF +  ++ KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  286 LVKDSATGLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTSLVGTGMNQTPVTLQVPGLMNSSMNQMGGIPTEVLCLMNMVSPEELLDDDEYEEIVEDVREECSKYGQVKSIEIPRPVDGLE--VPGTGKIFVEFMSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 470

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: puf60a (poly(U)-binding-splicing factor PUF60 [Source:NCBI gene;Acc:103042138])

HSP 1 Score: 65.0846 bits (157), Expect = 3.093e-10
Identity = 42/101 (41.58%), Postives = 59/101 (58.42%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ PE++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SE  KA++AL  R F GR V+   YD ER+ N
Sbjct:  415 QESTVMVLRNMVGPEDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEVIVKIFVEFSAASEMNKAIQALNNRWFGGRKVIAEVYDQERFDN 510

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Match: puf60a (poly(U)-binding-splicing factor PUF60 [Source:NCBI gene;Acc:103042138])

HSP 1 Score: 65.0846 bits (157), Expect = 3.267e-10
Identity = 42/101 (41.58%), Postives = 59/101 (58.42%), Query Frame = 3

Query: 1929 QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRN 2231
            QE+ ++ L NM+ PE++ DD E E   E     C K+G+VN + I +    EE+    + KI+VEFS  SE  KA++AL  R F GR V+   YD ER+ N
Sbjct:  454 QESTVMVLRNMVGPEDIDDDLEGEVTEE-----CGKFGAVNRVIIYQEKQGEEEDAEVIVKIFVEFSAASEMNKAIQALNNRWFGGRKVIAEVYDQERFDN 549

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2a [Source:ZFIN;Acc:ZDB-GENE-050706-131])

HSP 1 Score: 146.362 bits (368), Expect = 1.637e-37
Identity = 92/179 (51.40%), Postives = 125/179 (69.83%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYR 2228
            +++DS+T  SKGYAFCEYAD +VTD+A  GL GMQLG+K+++VQRAS+GAKN          +  Q        +   G  TE+LCL NM+TPEEL DDEEYE+I+ED++EEC KYGSV S++IPRP    +    G GKI+VEF++  +  +A +AL+GR F+ RVVVT +Y+ E+Y 
Sbjct:  280 LVKDSATCFSKGYAFCEYADVNVTDQAIAGLNGMQLGDKKLIVQRASVGAKNANPTVVVQTPVTLQVPGLASSQVQHSGLPTEVLCLMNMVTPEELVDDEEYEDILEDIREECGKYGSVRSVEIPRPVNGLDIP--GCGKIFVEFASLLDCQRAQQALSGRKFANRVVVTQYYNLEKYH 456

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO48329 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJT7])

HSP 1 Score: 148.673 bits (374), Expect = 3.222e-39
Identity = 103/187 (55.08%), Postives = 130/187 (69.52%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAK-NPXXXXXXXXXXXXXXXXGMDPSMGTCG--QETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPT-GSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGLSKGYAFCEY D  +TD A  GL GMQLG+K+++VQRAS+GAK N        ++ A+ Q  G+D SM   G    TE+L L NM+TP+EL DDEE+EEI +DV+EECSKYG V S++IPRP  G  E    GVGKI+VEFS+  +  KA  AL GR F+ RVVVTS+Y PE Y    F
Sbjct:  147 LVKDSATGLSKGYAFCEYVDLGITDVAIQGLNGMQLGDKKLIVQRASVGAKQNLNNPQAMNMVPAQLQIPGLDISMAVPGAVAATEVLALMNMVTPDELGDDEEFEEIYDDVREECSKYGRVKSMEIPRPMEGLMEPP--GVGKIFVEFSSIDDAKKAAAALGGRKFANRVVVTSYYPPEEYHRRIF 331

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Match: EDO46306 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQK4])

HSP 1 Score: 51.6026 bits (122), Expect = 1.821e-6
Identity = 25/66 (37.88%), Postives = 39/66 (59.09%), Query Frame = 3

Query: 2028 CSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY 2225
            CSK+G+V+ + I +    EE+    + KI+VEF++  ET KA   L GR F GR V    Y+ +++
Sbjct:  432 CSKFGTVSRVVIYKEKQGEEEDAEVIVKIFVEFTSPDETDKATSNLDGRWFGGRAVKAEAYNEDKF 497

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2 [Source:NCBI gene;Acc:101165173])

HSP 1 Score: 144.821 bits (364), Expect = 1.634e-36
Identity = 98/185 (52.97%), Postives = 129/185 (69.73%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTC-GQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D ++ D+A  GL GMQLG+K+++VQRAS+G+KN  T  +        Q  G++ S+    G  TE+LCL NM+ PEEL DDEEYEEIVEDV+EEC KYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  258 LVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGSKN-ATLTSINQTPVTLQVPGLNSSVTQMGGLPTEVLCLMNMVAPEELLDDEEYEEIVEDVREECGKYGQVKSIEIPRPVDGLEVP--GTGKIFVEFTSVFDAQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 439

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: u2af2a (U2 small nuclear RNA auxiliary factor 2 [Source:NCBI gene;Acc:101165173])

HSP 1 Score: 144.821 bits (364), Expect = 3.097e-36
Identity = 98/185 (52.97%), Postives = 129/185 (69.73%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTC-GQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY-RNGFF 2240
            +++DS+TGLSKGYAFCEY D ++ D+A  GL GMQLG+K+++VQRAS+G+KN  T  +        Q  G++ S+    G  TE+LCL NM+ PEEL DDEEYEEIVEDV+EEC KYG V S++IPRP    E    G GKI+VEF++  +  KAM+ L GR F+ RVVVT + DP+ Y R  F+
Sbjct:  289 LVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGSKN-ATLTSINQTPVTLQVPGLNSSVTQMGGLPTEVLCLMNMVAPEELLDDEEYEEIVEDVREECGKYGQVKSIEIPRPVDGLEVP--GTGKIFVEFTSVFDAQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW 470

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: uhmk1 (U2AF homology motif kinase 1 [Source:NCBI gene;Acc:101158012])

HSP 1 Score: 63.929 bits (154), Expect = 6.142e-10
Identity = 30/71 (42.25%), Postives = 44/71 (61.97%), Query Frame = 3

Query: 2028 CSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            C KYGSV SL IP+          G G+++VE++N S++ +A + L GRTF G+ VV +FY    Y+ G+F
Sbjct:  375 CQKYGSVVSLLIPK-------ENPGKGQVFVEYANSSDSKEAQRLLTGRTFDGKFVVATFYPLSAYKRGYF 438

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: uhmk1 (U2AF homology motif kinase 1 [Source:NCBI gene;Acc:101158012])

HSP 1 Score: 63.5438 bits (153), Expect = 6.859e-10
Identity = 30/71 (42.25%), Postives = 44/71 (61.97%), Query Frame = 3

Query: 2028 CSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            C KYGSV SL IP+          G G+++VE++N S++ +A + L GRTF G+ VV +FY    Y+ G+F
Sbjct:  342 CQKYGSVVSLLIPK-------ENPGKGQVFVEYANSSDSKEAQRLLTGRTFDGKFVVATFYPLSAYKRGYF 405

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Match: PUF60 (poly(U) binding splicing factor 60 [Source:NCBI gene;Acc:101163935])

HSP 1 Score: 60.8474 bits (146), Expect = 6.226e-9
Identity = 40/98 (40.82%), Postives = 58/98 (59.18%), Query Frame = 3

Query: 1932 ETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY 2225
            E+ ++ L NM+ PE++ DD E E   E     C K+G+VN + I +    EE+  + + KI+VEFS  SE  KA++AL  R F GR VV   YD +R+
Sbjct:  410 ESTVMVLRNMVGPEDIDDDLEGEVTEE-----CGKFGTVNRVIIYQEKQGEEEDADIIVKIFVEFSAASEMNKAIQALNDRWFGGRKVVAEVYDQDRF 502

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000078029.1 (SMESG000078029.1)

HSP 1 Score: 299.286 bits (765), Expect = 1.541e-89
Identity = 180/183 (98.36%), Postives = 183/183 (100.00%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPTTAATTALLIARQQQLGMDPSMGTCGQETEILCLHNMITPEELTDDEEYEEIVEDVKEECSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF
Sbjct:  318 LVKDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPTTAATTALLIARQQQLGMDPSMGTCGQETEILCLHNMITPEELTDDEEYEEIVEDVKEECSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 500

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000018652.1 (SMESG000018652.1)

HSP 1 Score: 221.09 bits (562), Expect = 2.821e-62
Identity = 130/183 (71.04%), Postives = 158/183 (86.34%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGLSKGYAFCEYADHSVTDKAC+GLTGMQLGEKRIVVQRA LGAKN +   T  L++ +     MDP+  +  +ETE+LCLHNM+  ++L DDEEYEEI+EDVKEECSK+G+V S+QIPRPTG+ +D+ +GVGKI+VEF NKSETVKAMKAL+GRTFSG+VVVTSFYD E+YRNGFF
Sbjct:  297 LVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMM--MDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGA-DDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 476

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000018647.1 (SMESG000018647.1)

HSP 1 Score: 221.09 bits (562), Expect = 2.821e-62
Identity = 130/183 (71.04%), Postives = 158/183 (86.34%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKNPXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF 2240
            +++DS+TGLSKGYAFCEYADHSVTDKAC+GLTGMQLGEKRIVVQRA LGAKN +   T  L++ +     MDP+  +  +ETE+LCLHNM+  ++L DDEEYEEI+EDVKEECSK+G+V S+QIPRPTG+ +D+ +GVGKI+VEF NKSETVKAMKAL+GRTFSG+VVVTSFYD E+YRNGFF
Sbjct:  297 LVKDSTTGLSKGYAFCEYADHSVTDKACMGLTGMQLGEKRIVVQRACLGAKNASGIPTGPLMLTQPMM--MDPTKISNVEETEVLCLHNMVMIDDLRDDEEYEEIMEDVKEECSKFGTVLSVQIPRPTGA-DDNVSGVGKIFVEFYNKSETVKAMKALSGRTFSGKVVVTSFYDLEKYRNGFF 476

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000051274.1 (SMESG000051274.1)

HSP 1 Score: 142.51 bits (358), Expect = 8.905e-36
Identity = 91/181 (50.28%), Postives = 122/181 (67.40%), Query Frame = 3

Query: 1692 IIQDSSTGLSKGYAFCEYADHSVTDKACLGLTGMQLGEKRIVVQRASLGAKN---PXXXXXXXXXXXXXXXXGMDPSMGTCGQETEILCLHNMITPXXXXXXXXXXXXXXXXXXXCSKYGSVNSLQIPRPTGSEEDSRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERY 2225
            +++D +TGLSKGYAFCEY D SVTD AC GL GMQLG+K+I+VQRAS+GAK+   P                G    +G     T++LCL NM+  E+L D+EEYE++V+D+  EC KYG+V SL+IPRP  +      GVGKI+VEF++  +  KA+ AL GR F+ RVVVTS+YD ++Y
Sbjct:  272 LVKDGATGLSKGYAFCEYVDQSVTDIACAGLNGMQLGDKKIIVQRASIGAKHGLIPMMMPFVNANFESLIAAG---GVGA----TKVLCLMNMVDEEDLVDEEEYEDVVDDINSECGKYGTVVSLEIPRPNPTM--LVGGVGKIFVEFASVIDCQKAVAALTGRKFNNRVVVTSYYDLDKY 443

[Close]

BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 48.521 bits (114), Expect = 7.528e-10
Identity = 31/75 (41.33%), Postives = 46/75 (61.33%), Query Frame = -3

Query: 3384 QDIKEITNNCKLCKLFIPHFFXXXXXXXXXXXXXXXXXXLDFIGPLSRKSENHFIFVIVYEYSRYPFAFPCSNME 3608
            QDIK +  +C++ K   P F +     L + TR  ERLS+DF+GP+   + N +I V + E+SRY FA+P  +ME
Sbjct: 2677 QDIKNVLADCRIYKEIKPKFIKPLLGILITFTRPWERLSIDFVGPIQSSTPNKYILVAIDEFSRYQFAYPFHDME 2751

HSP 2 Score: 35.4242 bits (80), Expect = 7.528e-10
Identity = 16/31 (51.61%), Postives = 24/31 (77.42%), Query Frame = -1

Query: 3224 RGAQFKSQSFRNLL-SPGVVKSKTTPYHPQA 3313
            RG+QF+S+  ++ L   GV+KS+T PY+PQA
Sbjct: 2776 RGSQFESKIVKDFLHRNGVLKSRTMPYNPQA 2806

The following BLAST results are available for this feature:

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
U2AF2	4.239e-40	54.35	U2 small nuclear RNA auxiliary factor 2 [Source:HG...	[more]
U2AF2	1.747e-38	54.35	U2 small nuclear RNA auxiliary factor 2 [Source:HG...	[more]
U2AF2	5.923e-37	53.68	U2 small nuclear RNA auxiliary factor 2 [Source:HG...	[more]
PUF60	1.040e-11	43.56	poly(U) binding splicing factor 60 [Source:HGNC Sy...	[more]
PUF60	1.046e-11	43.56	poly(U) binding splicing factor 60 [Source:HGNC Sy...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
uaf-1	3.761e-32	50.84	Splicing factor U2AF 65 kDa subunit [Source:UniPr...	[more]
uaf-1	3.842e-32	50.84	Splicing factor U2AF 65 kDa subunit [Source:UniPr...	[more]
uaf-1	5.096e-32	50.84	Splicing factor U2AF 65 kDa subunit [Source:UniPr...	[more]
uaf-1	2.083e-24	49.33	Splicing factor U2AF 65 kDa subunit [Source:UniPr...	[more]
uaf-1	2.083e-24	49.33	Splicing factor U2AF 65 kDa subunit [Source:UniPr...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
U2af50	1.789e-40	52.46	gene:FBgn0005411 transcript:FBtr0307177	[more]
U2af50	7.483e-40	52.46	gene:FBgn0005411 transcript:FBtr0307178	[more]
U2af50	7.483e-40	52.46	gene:FBgn0005411 transcript:FBtr0074234	[more]
U2af50	8.506e-40	52.46	gene:FBgn0005411 transcript:FBtr0307176	[more]
LS2	2.185e-35	48.88	gene:FBgn0034834 transcript:FBtr0072052	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
u2af2b	5.165e-37	53.76	U2 small nuclear RNA auxiliary factor 2b [Source:Z...	[more]
u2af2b	5.365e-37	53.76	U2 small nuclear RNA auxiliary factor 2b [Source:Z...	[more]
u2af2a	8.453e-37	52.43	U2 small nuclear RNA auxiliary factor 2a [Source:Z...	[more]
u2af2a	8.782e-37	52.43	U2 small nuclear RNA auxiliary factor 2a [Source:Z...	[more]
u2af2b	1.728e-25	58.46	U2 small nuclear RNA auxiliary factor 2b [Source:Z...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mbp	1.627e-38	53.26	myelin basic protein [Source:Xenbase;Acc:XB-GENE-9...	[more]
mbp	2.941e-38	53.26	myelin basic protein [Source:Xenbase;Acc:XB-GENE-9...	[more]
mbp	3.957e-38	53.26	myelin basic protein [Source:Xenbase;Acc:XB-GENE-9...	[more]
puf60	6.581e-12	43.56	poly(U) binding splicing factor 60KDa [Source:NCBI...	[more]
puf60	1.213e-11	43.56	poly(U) binding splicing factor 60KDa [Source:NCBI...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
U2af2	2.910e-40	54.35	U2 small nuclear ribonucleoprotein auxiliary facto...	[more]
U2af2	1.199e-38	54.35	U2 small nuclear ribonucleoprotein auxiliary facto...	[more]
U2af2	3.705e-37	53.68	U2 small nuclear ribonucleoprotein auxiliary facto...	[more]
Puf60	1.190e-11	43.56	poly-U binding splicing factor 60 [Source:MGI Symb...	[more]
Puf60	1.306e-11	43.56	poly-U binding splicing factor 60 [Source:MGI Symb...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q24562\|U2AF2_DROME	7.501e-39	52.46	Splicing factor U2AF 50 kDa subunit OS=Drosophila ...	[more]
sp\|P26369\|U2AF2_MOUSE	2.592e-36	53.68	Splicing factor U2AF 65 kDa subunit OS=Mus musculu...	[more]
sp\|P26368\|U2AF2_HUMAN	2.844e-36	53.68	Splicing factor U2AF 65 kDa subunit OS=Homo sapien...	[more]
sp\|P90978\|U2AF2_CAEEL	6.483e-31	50.84	Splicing factor U2AF 65 kDa subunit OS=Caenorhabdi...	[more]
sp\|P90727\|U2AF2_CAEBR	3.931e-30	51.40	Splicing factor U2AF 65 kDa subunit OS=Caenorhabdi...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0B7A573	7.043e-43	57.92	Uncharacterized protein OS=Arion vulgaris OX=10286...	[more]
A0A1V9XTQ2	1.375e-41	52.72	Splicing factor U2AF 50 kDa subunit-like (Fragment...	[more]
A0A2C9JG43	1.635e-40	59.02	U2 snRNP auxiliary factor large subunit OS=Biompha...	[more]
A0A1D2N903	2.968e-40	55.74	U2 snRNP auxiliary factor large subunit OS=Orchese...	[more]
A0A2S2P6Q0	3.140e-40	52.46	Splicing factor U2AF subunit OS=Schizaphis graminu...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
u2af2b	4.494e-38	52.72	splicing factor U2AF 65 kDa subunit [Source:NCBI g...	[more]
u2af2b	6.797e-38	52.72	splicing factor U2AF 65 kDa subunit [Source:NCBI g...	[more]
u2af2a	1.230e-36	52.94	splicing factor U2AF 65 kDa subunit-like [Source:N...	[more]
puf60a	3.093e-10	41.58	poly(U)-binding-splicing factor PUF60 [Source:NCBI...	[more]
puf60a	3.267e-10	41.58	poly(U)-binding-splicing factor PUF60 [Source:NCBI...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
u2af2a	1.637e-37	51.40	U2 small nuclear RNA auxiliary factor 2a [Source:Z...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO48329	3.222e-39	55.08	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46306	1.821e-6	37.88	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
u2af2a	1.634e-36	52.97	U2 small nuclear RNA auxiliary factor 2 [Source:NC...	[more]
u2af2a	3.097e-36	52.97	U2 small nuclear RNA auxiliary factor 2 [Source:NC...	[more]
uhmk1	6.142e-10	42.25	U2AF homology motif kinase 1 [Source:NCBI gene;Acc...	[more]
uhmk1	6.859e-10	42.25	U2AF homology motif kinase 1 [Source:NCBI gene;Acc...	[more]
PUF60	6.226e-9	40.82	poly(U) binding splicing factor 60 [Source:NCBI ge...	[more]

BLAST of U2 snRNP auxiliary factor large subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000078029.1	1.541e-89	98.36	SMESG000078029.1	[more]
SMESG000018652.1	2.821e-62	71.04	SMESG000018652.1	[more]
SMESG000018647.1	2.821e-62	71.04	SMESG000018647.1	[more]
SMESG000051274.1	8.905e-36	50.28	SMESG000051274.1	[more]
SMESG000077977.1	7.528e-10	41.33	SMESG000077977.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30007069 ID=SMED30007069|Name=U2 snRNP auxiliary factor large subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=3712bp
GAGCCCATAAATATCGATAATAATCTCTTAATTTTTAAGAAAACCTTAGA
CCTTACAACGAACATGTTCGTCAAGACTTGATAGAATGCAAAAATGGATT
TCTATACTCTTCATTTGAAATCCATAGCTCCATGAAACTAATTCAAGGCA
TTGCATAACTTTCAAGGATATTCGTTTAATTCTTAATTATCGGTGCTTTT
ATCTTCCCGACACTCTCAATATTTATGGGCTCGAAACTGTATGAATCCAA
TTCTTGCAAAGTCATATCTTCAAATATAGTGTATTAAAAACTTGTCTGGG
TTTTCGACCTGATTTAGTTGGAGCTTTCTAACCATCTTTTTTATGAGAGG
GTAATGATTGTTCTCTGTATAATTATTTCTGGATTTACTCCATTCTGATA
ATGACTTCTTTAGTTTTTCCATTGATTGAACTTCTCTCAATTTTAACTCT
TTCCTTGTTTCTAATTCTTCAATTGAATGCTTTACGAAAGCAACCTTTGT
ATCCAATATAATCTGACTTAATCTCTTCATATTTTTGAATTCCACCGACA
AAAAGGCTTTGATTAACGTTTCATGAAATTCCGCAGGCTGTTGAGTTATT
TCAAAGAATCTTTTTGTAACTCTCCATTCGTTCCGATTGGCTTGATGTTT
GGATAAAATTCAGATGTTCTGACATTGCTGCCATGTTAAAAAGAGACAGG
TGTATAATATTTATTCTCACGAATCTCCAACACATTTTTATATAGCTCTT
CATTGCATAACATCTAAAAAGCGAGTTTATTGGTTTAATAATTATTGATA
CAGATACAAGCCTACCAACATTCAAACACAACCATCAAACATCATAAATA
GCATATGATCCTGAAACTAGAATTTTAACATTATTAAGCAATTGGAACAT
TTAATTACTTGTCATTTATTAAATGGTAACATCCTCTATATGGATATTAA
ACCTTGAAATTTATCGTTTTAAAAATAGAAACTGTTGTATTAATTACTTT
TAGTGATTAAAATTTGCAATTAAACTTCACCGTCATAATTGCCAATTCTT
AAATTTCTTAAATACCAGAGGAAGACACATTTCATCCAATTGAAATTTAG
AGAATAATTTCTATATAATTCAATTAGATCAAGAACTTAAAAACGCCCTG
AAATTATAAGATTTCTTGACATTTTTATAATTATTTTAATTTTTTTTGTC
TAATAATAACTAACTAATAATAACACTTAATTTTGTCAACATTGACAATT
TCCACCAATCTTCATTTGTTTTTAGAAATATATCAACATTTTGGTATATA
TTGATTTTTAATTTTTGACGCTCTGAGTATTTGTAATATAAAAAAAATTA
TTGATTAATAGTCTCAAAAATACATGTGGGTAAAGAAAATCGAAAAAATG
ATAACCATAATAATCAATAATTTCTGTCAACTTGTCTTATCTAGCTTTTT
CCCCGTGAGCCTATTATTTTTTTACAAAATTTCATTACAAATATATGCTG
AAACTTAATTTTTTTTTAATTTCAAGTCGTTTTAAAGTCATATTAAGGTC
AAATAAAGTAGGTCATATATTAAATTTATTTTAAACAAAGGATATCACGC
CCAATATACATTTTTATCATTAATTAATTTTTCTTATTGTTTATTGTGCT
AATGAGATCAATTTATCCAATTATACGACGATTGATAATAAATAATTCAG
GATTCATCAACTGGATTGTCAAAAGGTTATGCATTCTGCGAATATGCTGA
CCACTCTGTAACCGATAAAGCCTGTCTCGGTCTTACTGGTATGCAATTGG
GTGAAAAGCGTATTGTGGTTCAGCGGGCTAGTCTTGGTGCCAAAAATCCT
ACGACAGCAGCAACAACAGCATTATTGATTGCTCGTCAACAACAGCTGGG
AATGGATCCCTCCATGGGCACATGTGGTCAGGAAACGGAAATTCTATGTC
TTCACAATATGATTACGCCGGAAGAGTTAACTGACGATGAGGAATACGAA
GAAATTGTTGAAGACGTCAAAGAAGAGTGCTCAAAATATGGATCGGTAAA
CAGTCTTCAAATACCTCGACCTACCGGCAGTGAGGAGGATTCCAGAAATG
GAGTCGGGAAAATCTACGTGGAATTCTCAAATAAATCAGAAACAGTTAAA
GCTATGAAAGCTTTAGCTGGCAGAACATTCAGCGGTAGAGTTGTTGTCAC
TTCATTTTATGACCCAGAAAGATATCGGAATGGATTTTTTTAATTTTTAA
TTTTAAAAATGTTTTATTCGGGGTAATCAAAAGTGTAAGGCGGCCGTTCG
TCGAATAATATTGATTGTGTAATTATGTTGAATCGTAATTGTAGTTTTTG
TAAAGCAATACATTCTACGAGATGATGAAAGCATTTTATAACTAGTCTAA
ACGGCGAATGATTTCGGAATGAATAATCATTACTCTTTCTAATTTTAAGA
TGTGCTTAAAATTTATATTAAAATTCCGATTTAATTTTCAAATTGGATAT
AATTTAATGCGAAATATTTTAGAGAATCTCTAATGTGTTCTGTTATTTAT
TGATTTCTGTTGAGTATTAAATAGTGGAGAAATAAGATTGGAAATGAAAT
TATTTCCAAAATTACTTCAGTTGTTTTAAAAATAATTTTGTTGTTTGATT
TACGATTCATAGAATATTTAGTCACTGTGGTGGAAATGGCAGGTTTGAAA
ACTTAATTTTCATGGAAATTAAAAAAATTTAATTTTACTGAAATTATTTC
ATGTAATATTTTTTGAAAATGGTCCAAGTGGGTTTTATTGTTGAGTTATA
AAAGACACATGTTTAAAAAGCTTAATTTTTATTATGAAGCGGTATTGAGC
CTGAATTTGATGACCGTACCTTTAGTTTCCGATTATAGTTCATCTAATGG
GCTTGAGGTGGTTGATGGTGTCAGATGACGTGTTGATACGGTGTGTACTT
TCCCATCTTTTTGTTGAATTCGGAAAAAATAAGTTTCCAGCAATTTTACT
TTGCTTACAATTTAAAAGATAAGTAAAATTTCTTGATTATTATTTCGTAG
ATATTCTGAAGGGTAATGGATGTTCTTTGCAAGTTGAACACAGATAAGAG
CTCTGATGGACAAAAATGCAGTGTGTACGAATTTTCCCGGTAGACCTTAT
CTAAATTATATTTCAAGAGTGCTAGGATTATTGTTTTGACGATTGCTAGG
TGCATTCACTCGCGTTGACCATTTGCTTGAGGATGATATGGAGTAGTTTT
ACTTTTTACTACTCCAGGACTTAGTAAGTTTCGAAATGATTGGCTCTTAA
ATTGTGCACCTCTACCGCTGTGAATACAGAATGAGAATATAGAGTGTGAA
AAGTTAAATTAAAAGTGTAATAAGGGTGCTCGTTTCCATATTGCTACAAG
GGAATGCGAATGGGTATCTCGAATATTCATATACGATTACGAAGATGAAG
TGATTTTCTGATTTTCTCGAGAGTGGACCAATGAAGTCTAATGATAATCT
TTCCAATGACCGTGTTGATGACCTTAATGAACTTGGAGGTAGTCTAAAGA
AATGTGGAATAAACAGTTTACAAAGCTTACAGTTATTTGTAATTTCTTTT
ATATCCTGTGTAATGGATCACATTTTTATTGCTTTTTTATTTAAATTTTT
TAAAAATAAAATTTATTCAATTCAAACAAATGTTAAATATCAAAACCTAT
TCATTCAATTCC

protein sequence of SMED30007069-orf-1

>SMED30007069-orf-1 ID=SMED30007069-orf-1|Name=SMED30007069-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp
MQLGEKRIVVQRASLGAKNPTTAATTALLIARQQQLGMDPSMGTCGQETE
ILCLHNMITPEELTDDEEYEEIVEDVKEECSKYGSVNSLQIPRPTGSEED
SRNGVGKIYVEFSNKSETVKAMKALAGRTFSGRVVVTSFYDPERYRNGFF
*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0008380	RNA splicing
GO:0006397	mRNA processing

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR035979	RBD_domain_sf
IPR012337	RNaseH-like_sf
IPR000504	RRM_dom
IPR012677	Nucleotide-bd_a/b_plait_sf

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0000166	nucleotide binding
GO:0003723	RNA binding

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	G3DSA:3.30.70.330		coord: 45..150 e-value: 1.6E-34 score: 120.0
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 74..135 e-value: 4.2E-7 score: 29.7
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 50..142 score: 10.343
None	No IPR available	PANTHER	PTHR23139	RNA-BINDING PROTEIN	coord: 1..147
None	No IPR available	PANTHER	PTHR23139:SF9	SPLICING FACTOR U2AF 65 KDA SUBUNIT	coord: 1..147
None	No IPR available	CDD	cd12232	RRM3_U2AF65	coord: 49..138 e-value: 1.82477E-43 score: 136.532
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	SSF54928	RNA-binding domain, RBD	coord: 48..145

Main menu

U2 snRNP auxiliary factor large subunit

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Owlery

blazegraph-runner