SMED30007056
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30007056 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Alignments
SMED30007056 aligns in the following genomic locations:
Homology
BLAST of SMED30007056 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 205.682 bits (522), Expect = 2.811e-62 Identity = 112/129 (86.82%), Postives = 113/129 (87.60%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENS +SERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 259 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENS----------------NSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 371 HSP 2 Score: 182.57 bits (462), Expect = 3.043e-54 Identity = 106/142 (74.65%), Postives = 112/142 (78.87%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNS----------IPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPP---KLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNS IPNENPE+ KL NS FREMQSI LNEN SK NDIINNSHPLINLDSERSILSEIP L PINSS I+I+ LS +SPV NS ++PE Sbjct: 478 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNENPEITYKLSRENSTFREMQSIRLNENPSKINDIINNSHPLINLDSERSILSEIPEIISILSPINSSGIEIETLSEQSPVKNSLLLPE 619 HSP 3 Score: 90.5077 bits (223), Expect = 4.782e-22 Identity = 64/129 (49.61%), Postives = 72/129 (55.81%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIEN NQSNI DS LINKSQ+S R +S+KTFVL N+ +N S + + +REM P KL PINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 380 ISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSII----NEYREM-----------------------------------PSKLSPINSSSIDIQHLSNRSPVDNSSVIPE 469 HSP 4 Score: 67.3958 bits (163), Expect = 7.239e-14 Identity = 69/157 (43.95%), Postives = 91/157 (57.96%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRN---------SIPNENPEMLSKLPHTNSAFREMQSIS-LNENSSKTN----DIIN-------------NSHPLINLD-SERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIEN NQS+IIDS LIN SQ+S R +S+KTFVL N SI NE E+ S L +S+ E+Q++S EN +++N IIN N L N + + +SI++E L I+SS I+I L+ +S VDNSSVIPE Sbjct: 706 ISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAEDKNIADQSIINEISEIPSNLSSIDSSSVEIQALSEFIENRNQSNIDGSVIINEFQLPMECSGSNTNEPGLANHNIANKSIINENQSNLSSIDSSGIEILALTEQSQVDNSSVIPE 862 HSP 5 Score: 66.6254 bits (161), Expect = 1.026e-13 Identity = 76/180 (42.22%), Postives = 96/180 (53.33%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRN----------SIPNENPEMLSKLPHTN----------SAFREMQSIS------LNENSSKTNDIINNSHPLIN-LDSERSI------------------LSEIPPKLPPINSSSIDIQHLS------NRSPVDNSSVIPE 389 ISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N SI NEN +M SKL H N S F E +SIS LN++ + +INNS I L+S+++ +SEIP L I+SSS++IQ LS N+S +D S +I E Sbjct: 622 ISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKNLSNKSQVDNSSVFPE-KSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAEDKNIADQSIINEISEIPSNLSSIDSSSVEIQALSEFIENRNQSNIDGSVIINE 800 HSP 6 Score: 66.6254 bits (161), Expect = 1.098e-13 Identity = 41/65 (63.08%), Postives = 51/65 (78.46%), Query Frame = 3 Query: 201 SLNENSSKTNDIINNSH-PLINLDSERSILSE-IPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 S+ EN S IINN + L +++++SI++E +P KL PINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 186 SILENCSIDKSIINNYYIDLHKINADKSIINENMPSKLHPINSSSIDIQHLSNRSPVDNSSVIPE 250
BLAST of SMED30007056 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 205.682 bits (522), Expect = 2.811e-62 Identity = 112/129 (86.82%), Postives = 113/129 (87.60%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENS +SERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 260 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENS----------------NSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 372 HSP 2 Score: 182.57 bits (462), Expect = 2.899e-54 Identity = 106/142 (74.65%), Postives = 112/142 (78.87%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNS----------IPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPP---KLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNS IPNENPE+ KL NS FREMQSI LNEN SK NDIINNSHPLINLDSERSILSEIP L PINSS I+I+ LS +SPV NS ++PE Sbjct: 479 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNENPEITYKLSRENSTFREMQSIRLNENPSKINDIINNSHPLINLDSERSILSEIPEIISILSPINSSGIEIETLSEQSPVKNSLLLPE 620 HSP 3 Score: 90.5077 bits (223), Expect = 4.645e-22 Identity = 64/129 (49.61%), Postives = 72/129 (55.81%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIEN NQSNI DS LINKSQ+S R +S+KTFVL N+ +N S + + +REM P KL PINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 381 ISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSII----NEYREM-----------------------------------PSKLSPINSSSIDIQHLSNRSPVDNSSVIPE 470 HSP 4 Score: 67.3958 bits (163), Expect = 7.170e-14 Identity = 69/157 (43.95%), Postives = 91/157 (57.96%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRN---------SIPNENPEMLSKLPHTNSAFREMQSIS-LNENSSKTN----DIIN-------------NSHPLINLD-SERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIEN NQS+IIDS LIN SQ+S R +S+KTFVL N SI NE E+ S L +S+ E+Q++S EN +++N IIN N L N + + +SI++E L I+SS I+I L+ +S VDNSSVIPE Sbjct: 707 ISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAEDKNIADQSIINEISEIPSNLSSIDSSSVEIQALSEFIENRNQSNIDGSVIINEFQLPMECSGSNTNEPGLANHNIANKSIINENQSNLSSIDSSGIEILALTEQSQVDNSSVIPE 863 HSP 5 Score: 67.0106 bits (162), Expect = 9.775e-14 Identity = 76/180 (42.22%), Postives = 96/180 (53.33%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRN----------SIPNENPEMLSKLPHTN----------SAFREMQSIS------LNENSSKTNDIINNSHPLIN-LDSERSI------------------LSEIPPKLPPINSSSIDIQHLS------NRSPVDNSSVIPE 389 ISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N SI NEN +M SKL H N S F E +SIS LN++ + +INNS I L+S+++ +SEIP L I+SSS++IQ LS N+S +D S +I E Sbjct: 623 ISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKNLSNKSQVDNSSVFPE-KSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAEDKNIADQSIINEISEIPSNLSSIDSSSVEIQALSEFIENRNQSNIDGSVIINE 801 HSP 6 Score: 66.6254 bits (161), Expect = 1.087e-13 Identity = 41/65 (63.08%), Postives = 51/65 (78.46%), Query Frame = 3 Query: 201 SLNENSSKTNDIINNSH-PLINLDSERSILSE-IPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 S+ EN S IINN + L +++++SI++E +P KL PINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 187 SILENCSIDKSIINNYYIDLHKINADKSIINENMPSKLHPINSSSIDIQHLSNRSPVDNSSVIPE 251
BLAST of SMED30007056 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 205.682 bits (522), Expect = 2.811e-62 Identity = 112/129 (86.82%), Postives = 113/129 (87.60%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENS +SERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 264 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENS----------------NSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 376 HSP 2 Score: 182.57 bits (462), Expect = 3.014e-54 Identity = 106/142 (74.65%), Postives = 112/142 (78.87%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNS----------IPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPP---KLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNS IPNENPE+ KL NS FREMQSI LNEN SK NDIINNSHPLINLDSERSILSEIP L PINSS I+I+ LS +SPV NS ++PE Sbjct: 483 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNENPEITYKLSRENSTFREMQSIRLNENPSKINDIINNSHPLINLDSERSILSEIPEIISILSPINSSGIEIETLSEQSPVKNSLLLPE 624 HSP 3 Score: 90.5077 bits (223), Expect = 4.782e-22 Identity = 64/129 (49.61%), Postives = 72/129 (55.81%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSSKTNDIINNSHPLINLDSERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIEN NQSNI DS LINKSQ+S R +S+KTFVL N+ +N S + + +REM P KL PINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 385 ISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSII----NEYREM-----------------------------------PSKLSPINSSSIDIQHLSNRSPVDNSSVIPE 474 HSP 4 Score: 67.3958 bits (163), Expect = 7.240e-14 Identity = 69/157 (43.95%), Postives = 91/157 (57.96%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRN---------SIPNENPEMLSKLPHTNSAFREMQSIS-LNENSSKTN----DIIN-------------NSHPLINLD-SERSILSEIPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 ISNEFIEN NQS+IIDS LIN SQ+S R +S+KTFVL N SI NE E+ S L +S+ E+Q++S EN +++N IIN N L N + + +SI++E L I+SS I+I L+ +S VDNSSVIPE Sbjct: 711 ISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAEDKNIADQSIINEISEIPSNLSSIDSSSVEIQALSEFIENRNQSNIDGSVIINEFQLPMECSGSNTNEPGLANHNIANKSIINENQSNLSSIDSSGIEILALTEQSQVDNSSVIPE 867 HSP 5 Score: 66.6254 bits (161), Expect = 1.046e-13 Identity = 76/180 (42.22%), Postives = 96/180 (53.33%), Query Frame = 3 Query: 3 ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRN----------SIPNENPEMLSKLPHTN----------SAFREMQSIS------LNENSSKTNDIINNSHPLIN-LDSERSI------------------LSEIPPKLPPINSSSIDIQHLS------NRSPVDNSSVIPE 389 ISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N SI NEN +M SKL H N S F E +SIS LN++ + +INNS I L+S+++ +SEIP L I+SSS++IQ LS N+S +D S +I E Sbjct: 627 ISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKNLSNKSQVDNSSVFPE-KSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAEDKNIADQSIINEISEIPSNLSSIDSSSVEIQALSEFIENRNQSNIDGSVIINE 805 HSP 6 Score: 66.6254 bits (161), Expect = 1.109e-13 Identity = 41/65 (63.08%), Postives = 51/65 (78.46%), Query Frame = 3 Query: 201 SLNENSSKTNDIINNSH-PLINLDSERSILSE-IPPKLPPINSSSIDIQHLSNRSPVDNSSVIPE 389 S+ EN S IINN + L +++++SI++E +P KL PINSSSIDIQHLSNRSPVDNSSVIPE Sbjct: 191 SILENCSIDKSIINNYYIDLHKINADKSIINENMPSKLHPINSSSIDIQHLSNRSPVDNSSVIPE 255 The following BLAST results are available for this feature:
BLAST of SMED30007056 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30007056 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30007056 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30007056 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30007056 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30007056 ID=SMED30007056|Name=SMED30007056|organism=Schmidtea mediterranea sexual|type=transcript|length=390bpback to top protein sequence of SMED30007056-orf-1 >SMED30007056-orf-1 ID=SMED30007056-orf-1|Name=SMED30007056-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp ISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLback to top InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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