Gag-pol fusion protein

Overview
NameGag-pol fusion protein
Smed IDSMED30007028
Length (bp)2766
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Gag-pol fusion protein (SMED30007028) t-SNE clustered cells

Violin plots show distribution of expression levels for Gag-pol fusion protein (SMED30007028) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Gag-pol fusion protein (SMED30007028) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Gag-pol fusion protein (SMED30007028) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30007028

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30007028 SmedASXL_013352SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30007028 SmedASXL_013352SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30007028 dd_Smed_v4_3842_4_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30007028 dd_Smed_v4_3842_4_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30007028 dd_Smed_v4_3842_4_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30007028 dd_Smed_v4_3842_4_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Zebrafish
Match: BX890543.1 (pep chromosome:GRCz11:14:42224893:42231293:-1 gene:ENSDARG00000114583.1 transcript:ENSDART00000185486.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX890543.1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.139e-21
Identity = 46/153 (30.07%), Postives = 75/153 (49.02%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDL-GDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWG--RKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFV 608
            ++ P   +QF+V  +A   G+GAVL QR  L G  +  A+ S  L P  +NY V   E LA+  A+ ++R +L G  +  +V+TDH  LE +      T +  R AL    +   + +     N   DA+SR+ +   +E   +  L + + V
Sbjct:  800 LSIPDPEQQFIVEVDASDVGVGAVLSQRSCLDGKVHPCAFFSHRLNPSERNYDVGNRELLAVRLALGEWRHWLEGAAQPFLVWTDHKNLEYIRSARRLTPRQARWALFFDRFKFTLSFRPGTKNVKPDALSRLFEVPGKEKSVDAILPKEMVV 952

HSP 2 Score: 56.225 bits (134), Expect = 3.139e-21
Identity = 27/52 (51.92%), Postives = 31/52 (59.62%), Query Frame = 1 
Query:   16 YYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            +YR+FIR F  IA PLT L    V F W S+ QEAFD LK   V  P+L  P
Sbjct:  752 FYRRFIRNFGQIAAPLTALTSPKVWFKWNSDAQEAFDELKSRFVSAPVLSIP 803
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.931e-21
Identity = 48/150 (32.00%), Postives = 73/150 (48.67%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDL-GDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFV 608
            P  ++QF+V  +A   G+GA+L QR    G  +  AY S  L    +NY +   E LA+  A+ ++R +L G     IV+TDH  LE +       S+  R AL    +D  I Y   + N   DA+SR+    E+    E  + R +F+
Sbjct:  814 PDPSRQFVVEVDASEVGVGAILSQRSSSDGKMHPCAYFSHRLNNAEQNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIQSAKRLNSRQARWALFFGRFDFSISYRPGSKNVKPDALSRIFDHSERASSPETIVPRRLFI 963

HSP 2 Score: 50.0618 bits (118), Expect = 4.931e-21
Identity = 25/52 (48.08%), Postives = 29/52 (55.77%), Query Frame = 1 
Query:   16 YYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            +YR+FIR FS +A PLT L      F W +  Q AFD LK   V  PIL  P
Sbjct:  763 FYRRFIRNFSQLAAPLTALTSLKTPFRWSNAAQVAFDRLKSCFVSAPILIAP 814
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.326e-19
Identity = 44/130 (33.85%), Postives = 66/130 (50.77%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDL-GDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P  ++QF+V  +A   G+GA+L QR    G  +  AY S  L P  +NY +   E LA+  A+ ++R +L G     IV+TDH  LE +       S+  R AL    ++  I Y   + N   DA+SR+
Sbjct:  788 PDPSRQFVVEVDASEVGVGAILSQRSSSDGKIHPCAYFSHRLSPAERNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIRSAKRLNSRQARWALFFGRFNFTISYRPGSKNIKPDALSRL 917

HSP 2 Score: 49.2914 bits (116), Expect = 1.326e-19
Identity = 24/52 (46.15%), Postives = 29/52 (55.77%), Query Frame = 1 
Query:   16 YYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            +YR+FIR FS +A PLT L    + F W S  + AF  LK   V  PIL  P
Sbjct:  737 FYRRFIRNFSQLAAPLTSLTSSKMPFRWSSAAEAAFSKLKGCFVSAPILIAP 788
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Zebrafish
Match: CR855320.1 (pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENSDARG00000099359.2 transcript:ENSDART00000159655.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR855320.1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.954e-19
Identity = 46/150 (30.67%), Postives = 71/150 (47.33%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDL-GDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFV 608
            P  ++QF+V  +A   G+GA+L QR    G  +  AY S  L     NY +   E LA+  A+ ++R +L G     IV+TDH  LE +       S+  R AL    ++  I Y   + N   DA+SR+    E+    E  + + I +
Sbjct:  816 PDPSRQFVVEVDASEVGVGAILSQRSSSDGKIHPCAYYSHRLSAAESNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIKSAKRLNSRQARWALFFGRFNFTISYRPGSKNIKPDALSRLFDSSERTSSLEPVVPKRIVI 965

HSP 2 Score: 48.9062 bits (115), Expect = 9.954e-19
Identity = 24/52 (46.15%), Postives = 28/52 (53.85%), Query Frame = 1 
Query:   16 YYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            +YR+FIR FS +A PLT L      F W S  + AF  LK   V  PIL  P
Sbjct:  765 FYRRFIRNFSQLAAPLTALTSSKTPFRWSSAAEAAFSKLKGCFVSAPILITP 816
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Zebrafish
Match: CR925755.2 (pep chromosome:GRCz11:17:42486740:42492668:-1 gene:ENSDARG00000116402.1 transcript:ENSDART00000183946.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR925755.2)

HSP 1 Score: 67.0106 bits (162), Expect = 2.242e-18
Identity = 46/150 (30.67%), Postives = 73/150 (48.67%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDL-GDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFV 608
            P  ++QF+V  +    G+GA+L QR  L G  +  AY S  L    +NY +   E LA+  A+ ++R +L G     IV TDH  LE +       S+  R AL    ++  I Y   + N   DA+SR+  + ++    +  L + +FV
Sbjct:  787 PDPSRQFVVEVDVSEVGVGAILSQRSALDGKIHPCAYFSHRLSAAERNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVSTDHKNLEYIKSAKRLNSRQARWALFFGRFNFSISYRPGSKNIKPDALSRLFDRSDRTSSPDPVLPQRVFV 936

HSP 2 Score: 46.2098 bits (108), Expect = 2.242e-18
Identity = 22/52 (42.31%), Postives = 27/52 (51.92%), Query Frame = 1 
Query:   16 YYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            +YR+FIR F  +A PLT L      F W +  + AF  LK   V  PIL  P
Sbjct:  736 FYRRFIRNFRQLAAPLTNLTSSKTPFRWSNAAEAAFSKLKGCFVSAPILIAP 787
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Xenopus
Match: ENSXETT00000024810.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464121.1:1437:3201:1 gene:ENSXETG00000014958.1 transcript:ENSXETT00000024810.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 112.464 bits (280), Expect = 2.026e-30
Identity = 59/131 (45.04%), Postives = 81/131 (61.83%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQD 560
            P F++ F+V T+A T GIGAVL Q D+ G E+ I Y SR L P    YA IE E LAIV+A+ + + YL+G    V TDH PL  L + + D  KL+R +L LQ ++  I + K + + NAD +SR D +D
Sbjct:  178 PDFSRGFIVHTDASTYGIGAVLSQVDEKGGEHPIIYLSRKLLPREVAYATIEKECLAIVWALKKLQPYLFGSAFTVVTDHNPLSWLQRVSGDNGKLLRWSLALQQFNFTIQHRKGSHHGNADGLSRRDGED 308

HSP 2 Score: 41.9726 bits (97), Expect = 2.026e-30
Identity = 25/56 (44.64%), Postives = 28/56 (50.00%), Query Frame = 1 
Query:   10 VGYYRKFIRIFSTIAFPLTQLEGKNVKFV--WGSEQQEAFDTLKKLLVEPPILPFP 171
             GYYR+FI  +S IA PLT L  K    V  W  E   A   LK  LV  P+L  P
Sbjct:  123 AGYYRRFIPNYSAIAKPLTDLTSKRRPRVVTWTPECATAMSALKSALVNAPVLYAP 178
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Xenopus
Match: ENSXETT00000011934.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:51966053:51967075:1 gene:ENSXETG00000005538.1 transcript:ENSXETT00000011934.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.9745 bits (232), Expect = 1.702e-25
Identity = 50/127 (39.37%), Postives = 76/127 (59.84%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P+F K+FLV T+A   G+GAVL Q  + G+E+ +AY SR L P    YA++E E LAI +A+   R YL GR+  + TDH PL+ + ++    +++ R  L LQ +   + +       NADA+SR+
Sbjct:  102 PNFKKEFLVQTDASEVGLGAVLSQVVN-GEEHPVAYLSRKLTPAECRYAIVERECLAIKWALESLRYYLLGRQFKLITDHAPLKWMAQNREKNARVTRWFLSLQNFKFSVEHRPGKLQGNADALSRV 227

HSP 2 Score: 43.8986 bits (102), Expect = 1.702e-25
Identity = 24/58 (41.38%), Postives = 32/58 (55.17%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLE--GKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             +VGYYR+F+  F+++A PLT L    K     W  E +EAF  LK  L   P+L  P
Sbjct:   45 GIVGYYRRFVPNFASLASPLTDLTKGSKAGAITWTPEAEEAFLNLKASLCRYPVLIAP 102
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 114.005 bits (284), Expect = 3.195e-25
Identity = 55/140 (39.29%), Postives = 88/140 (62.86%), Query Frame = 3 
Query:  132 QEIIGGTSNITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMD 551
            ++ +  +  +  P F+++F++ T+A   G+GAVL Q +  G+E+ +AY SR L P    YA IE E LAIV+A+ + + YL+GR+  V TDH PL  L + + D  KL+R +L+LQ Y+  I + K  ++ NAD +SR +
Sbjct:  616 KQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQVNTYGEEHPVAYLSRKLLPREAAYATIEKECLAIVWALQKLQPYLYGREFTVVTDHNPLSWLQRVSGDNGKLLRWSLLLQQYNFTIQHRKGKEHHNADGLSRQE 755
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Xenopus
Match: ENSXETT00000031144.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:8:63282574:63284044:1 gene:ENSXETG00000009810.1 transcript:ENSXETT00000031144.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 3.094e-13
Identity = 48/136 (35.29%), Postives = 68/136 (50.00%), Query Frame = 3 
Query:  180 KQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAIT-QFRTYLWGRKLIVYTDH*PLE-LLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKE 581
            + +++  +A   G+G VL QR   G    +AY SR L P  KNY V +LE LA+ +AI  +   +L+G +  V TD+ PL  +LT   +D +    LA  L  Y   + Y     N  ADA+SR       ED  E
Sbjct:  124 RPYVLHVDASYEGLGGVLHQRYPEGLR-PVAYLSRSLAPSEKNYPVHKLEFLALKWAIVDKLHDFLYGVEFEVRTDNNPLTYILTTAKLDATGHRWLA-ALSNYSFTLKYKPGPRNIGADALSRRPGLPALEDDGE 257

HSP 2 Score: 38.891 bits (89), Expect = 3.094e-13
Identity = 23/69 (33.33%), Postives = 32/69 (46.38%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQL------EGKNVK--------FVWGSEQQEAFDTLKKLLVEPPILPF 168
               GYYR+F+  +S +A PL +L       G+  K          W S  + AF  LKK L E P+L +
Sbjct:   51 GFCGYYRRFVEGYSRVAHPLNELLRLSNVHGEGTKRDAKAPFGDKWTSACEGAFVQLKKRLTEAPVLAY 119
[Close]
BLAST of Gag-pol fusion protein vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.199e-27
Identity = 51/130 (39.23%), Postives = 72/130 (55.38%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            + +P F+K+F + T+A     GAVL Q  + G +  +AYASR       N +  E E  AI +AI  FR Y++G+   V TDH PL  L      +SKL R+ L L+ Y+  + Y K  DN  ADA+SR+
Sbjct:  607 LQYPDFSKEFCITTDASKQACGAVLTQNHN-GHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYLFSMVNPSSKLTRIRLELEEYNFTVEYLKGKDNHVADALSRI 735

HSP 2 Score: 50.0618 bits (118), Expect = 5.199e-27
Identity = 24/56 (42.86%), Postives = 34/56 (60.71%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            A   YYR+FI+ F+  +  +T+L  KNV F W  E Q+AF  LK  L+ P +L +P
Sbjct:  555 AFCNYYRRFIKNFADYSRHITRLCKKNVPFEWTDECQKAFIHLKSQLINPTLLQYP 610
[Close]
BLAST of Gag-pol fusion protein vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 8.108e-25
Identity = 53/127 (41.73%), Postives = 71/127 (55.91%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P F K+F + T+A    +GAVL Q     D + ++Y SR L  H  NY+ IE E LAIV+A   FR YL GR   + +DH PL  L +     SKL R  + L  +D +I Y K  +N  ADA+SR+
Sbjct:  503 PDFTKKFTLTTDASDVALGAVLSQ-----DGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWLYRMKDPNSKLTRWRVKLSEFDFDIKYIKGKENCVADALSRI 624

HSP 2 Score: 46.595 bits (109), Expect = 8.108e-25
Identity = 27/57 (47.37%), Postives = 32/57 (56.14%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKF-VWGSEQQEAFDTLKKLLVEPPILPFP 171
             L GYYRKFI  F+ IA P+T+   KN+K      E   AF  LK L+ E PIL  P
Sbjct:  447 GLTGYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVP 503
[Close]
BLAST of Gag-pol fusion protein vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 5.209e-23
Identity = 51/130 (39.23%), Postives = 73/130 (56.15%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            +  P F K+F++ T+A    +GAVL Q     + + I++ SR L  H  NY+ IE E LAIV+A   FR YL GR+ ++ +DH PL  L       +KL R  + L  Y  +I Y K  +N  ADA+SR+
Sbjct:  499 LQLPDFEKKFVLTTDASNLALGAVLSQ-----NGHPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGRQFLIASDHQPLRWLHNLKEPGAKLERWRVRLSEYQFKIDYIKGKENSVADALSRI 623

HSP 2 Score: 43.8986 bits (102), Expect = 5.209e-23
Identity = 25/57 (43.86%), Postives = 31/57 (54.39%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKF-VWGSEQQEAFDTLKKLLVEPPILPFP 171
             L GYYRKFI  ++ IA P+T    K  K      E  EAF+ LK L++  PIL  P
Sbjct:  446 GLTGYYRKFIPNYADIAKPMTSCLKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLP 502
[Close]
BLAST of Gag-pol fusion protein vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 8.715e-19
Identity = 62/163 (38.04%), Postives = 85/163 (52.15%), Query Frame = 3 
Query:   81 KCEICMGLRATRSVRYTQEIIGGTSNITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLI-VYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQ 566
            K  I +   A +S    + I+  +  + FP F K F + T+A    IGAVL Q DD G +  IAY SR L    +NYA IE E LAI++++   R YL+G   I VYTDH PL     +    +KL R    ++ Y+ E+ Y     N  ADA+SR+  Q  Q
Sbjct:  400 KVPITLDETALQSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQ-DDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGAGTIKVYTDHQPLTFALGNRNFNAKLKRWKARIEEYNCELIYKPGKSNVVADALSRIPPQLNQ 561
[Close]
BLAST of Gag-pol fusion protein vs. UniProt/SwissProt
Match: sp|P10401|POLY_DROME (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.463e-12
Identity = 50/158 (31.65%), Postives = 83/158 (52.53%), Query Frame = 3 
Query:  114 RSVRYTQEIIGGTSNITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWG-RKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMD----QQDEQED 572
            R++  ++++I     + +P F K F + T+A   GIGAVL Q     +   I   SR LK   +NYA  E E LAIV+A+ + + +L+G R++ ++TDH PL          +K+ R    +  ++ ++ Y    +N  ADA+SR +    Q + Q D
Sbjct:  476 RNILASEDVI-----LKYPDFKKPFDLTTDASASGIGAVLSQ-----EGRPITMISRTLKQPEQNYATNERELLAIVWALGKLQNFLYGSREINIFTDHQPLTFAVADRNTNAKIKRWKSYIDQHNAKVFYKPGKENFVADALSRQNLNALQNEPQSD 623
[Close]
BLAST of Gag-pol fusion protein vs. TrEMBL
Match: A0A355ABF2 (Uncharacterized protein OS=Flavobacteriaceae bacterium OX=1871037 GN=DDZ39_05755 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.948e-41
Identity = 73/163 (44.79%), Postives = 106/163 (65.03%), Query Frame = 3 
Query:  165 FPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQ-----------DEQEDVKEGRLERTIFVVLRE 620
            FP   K F++MT+A    IGAVLGQ+D++  ++ IAYASR LK H  NY+VIE EALAIVY++ QF  Y+W RK+I+YTD  PL+ L  H   +S+L+R +L+LQ YDI+I Y +   N NAD +SRMD+            D+ E ++  R ++ +F ++ +
Sbjct: 1638 FPDMNKDFIIMTDASGYAIGAVLGQKDEMLKDHVIAYASRILKSHEVNYSVIEKEALAIVYSVKQFHHYIWSRKIILYTDQRPLQWLMTHKDSSSRLIRWSLLLQEYDIDIKYRQGKANANADFLSRMDEPVQCMVSMLANFDKNELLQAQRADKGLFYIIED 1800

HSP 2 Score: 55.4546 bits (132), Expect = 1.948e-41
Identity = 26/56 (46.43%), Postives = 34/56 (60.71%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             + GYYRKFI  F+ IA PL  L  KN   +W  + QE+F+ LK  L+  P+L FP
Sbjct: 1584 GMAGYYRKFIPNFAKIASPLFDLTKKNDNTLWTEKHQESFEELKNRLIHFPVLRFP 1639
[Close]
BLAST of Gag-pol fusion protein vs. TrEMBL
Match: A0A1X7UNW9 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 8.553e-35
Identity = 57/133 (42.86%), Postives = 83/133 (62.41%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQ 557
            + +P F+  F++ T+A   G+GAVL Q ++ G  + +AYASR +  H KNY + ELEAL +V+A+  FR YLWG K  VYTDH P++ L +    + KL R + ++  YD++I Y     N NADA+SR   Q
Sbjct:  124 LAYPDFSNPFILHTDASGEGLGAVLEQSNENGVCHPVAYASRTVSEHEKNYGITELEALGVVWALKHFRAYLWGHKTTVYTDHSPVKSLLRAKHTSGKLARWSQVVSEYDLDICYRPGRQNSNADALSRAPLQ 256

HSP 2 Score: 58.5362 bits (140), Expect = 8.553e-35
Identity = 29/54 (53.70%), Postives = 33/54 (61.11%), Query Frame = 1 
Query:   10 VGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            V YYRKF+  F+ IA PL QL  KNV F W    Q AF  LK LL  PP+L +P
Sbjct:   74 VDYYRKFVPGFARIASPLHQLLKKNVPFQWTGACQSAFQKLKDLLTSPPVLAYP 127
[Close]
BLAST of Gag-pol fusion protein vs. TrEMBL
Match: A0A2H5TX34 (Gag-pol fusion protein OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=RIR_2940700 PE=4 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 9.820e-35
Identity = 55/130 (42.31%), Postives = 79/130 (60.77%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            + +P+F K F+V T+A T  +GA+L Q+D+  +E  IAYASR L  H +NY + ELE LA++++I  F  YL G+K +V TDH  L+ L K T    KL R  + L  YD+EI       + N D +SR+
Sbjct: 1216 LAYPNFEKPFMVFTDASTYALGAILAQKDENNNECVIAYASRTLNKHERNYGITELECLAVIWSIRHFHHYLHGQKFVVITDHAALKYLLKMTNPVGKLGRWLMTLNGYDLEIINRPGKQHSNVDTLSRI 1345

HSP 2 Score: 67.781 bits (164), Expect = 9.820e-35
Identity = 31/56 (55.36%), Postives = 39/56 (69.64%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
            AL  YYRKF+  FS IA PL +L  KNV ++W S+QQ+AF+ LK  L  PPIL +P
Sbjct: 1164 ALASYYRKFVNNFSKIAEPLHRLLKKNVPYIWASDQQKAFENLKICLTTPPILAYP 1219
[Close]
BLAST of Gag-pol fusion protein vs. TrEMBL
Match: A0A2H5S029 (Retrotransposable element OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=RIR_1049600 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.566e-34
Identity = 56/127 (44.09%), Postives = 76/127 (59.84%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P + K+FL++T+A   G+GAVL Q+D+ G E  IAYASR L P  +NY + ELE L IV+ I  F  YL  RK  V TDH  L+ L    +   K  R  + LQ Y+ E+ +    +N NADA+SR+
Sbjct: 1684 PDWKKEFLLITDASGKGLGAVLSQKDEKGKEVVIAYASRSLLPAEENYPITELECLGIVWGIQHFHKYLIDRKFKVITDHSALKGLMNAKIPKGKRARWVMELQQYNFEVIHRSGKENTNADALSRL 1810

HSP 2 Score: 68.5514 bits (166), Expect = 1.566e-34
Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYRKF++ FS IA P++ L  K V F+WG EQQEAF+ LK+ L++ PIL  P
Sbjct: 1629 GLCSYYRKFVKNFSKIARPISDLRKKGVPFIWGKEQQEAFEKLKEKLIQYPILRHP 1684
[Close]
BLAST of Gag-pol fusion protein vs. TrEMBL
Match: A0A2H5RIZ5 (Retrotransposable element OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=RIR_0590300 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.893e-34
Identity = 56/127 (44.09%), Postives = 76/127 (59.84%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P + K+FL++T+A   G+GAVL Q+D+ G E  IAYASR L P  +NY + ELE L IV+ I  F  YL  RK  V TDH  L+ L    +   K  R  + LQ Y+ E+ +    +N NADA+SR+
Sbjct: 1683 PDWKKEFLLITDASGKGLGAVLSQKDEKGKEVVIAYASRSLLPAEENYPITELECLGIVWGIQHFHKYLIDRKFKVITDHSALKGLMNAKIPKGKRARWVMELQQYNFEVIHRSGKENTNADALSRL 1809

HSP 2 Score: 68.5514 bits (166), Expect = 1.893e-34
Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYRKF++ FS IA P++ L  K V F+WG EQQEAF+ LK+ L++ PIL  P
Sbjct: 1628 GLCSYYRKFVKNFSKIARPISDLRKKGVPFIWGREQQEAFEKLKEKLIQYPILRHP 1683
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Cavefish
Match: ENSAMXT00000041345.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:32334536:32339002:1 gene:ENSAMXG00000029230.1 transcript:ENSAMXT00000041345.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.908 bits (250), Expect = 1.405e-28
Identity = 54/130 (41.54%), Postives = 78/130 (60.00%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            +T P+F++ F + T+A   G+GAVL Q  D G E+ +AYASR L+   +NY+  E E LA+V+A+ ++R YL GR   V TDH  L  +  H   TS+L R A+ LQ +D  + Y K   N   D +SR+
Sbjct:  915 LTPPNFSEPFQIQTDASDQGLGAVLSQGTD-GLEHVVAYASRLLQGAERNYSTAEKECLAVVWAVEKWRVYLEGRHFTVITDHSALSWVFNHPKPTSRLTRWAIRLQTFDFSVQYRKGKCNIVPDTLSRI 1043

HSP 2 Score: 46.2098 bits (108), Expect = 1.405e-28
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             + G+Y +FI  FS  A  L  L+ KN  ++W  E Q+AF+ +K+ L+  P+L  P
Sbjct:  863 GMAGWYHRFITHFSERAAILNALKKKNAPWIWTQECQKAFEDIKQALITAPVLTPP 918
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Cavefish
Match: ENSAMXT00000051014.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001022.1:118636:123375:-1 gene:ENSAMXG00000038578.1 transcript:ENSAMXT00000051014.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.0485 bits (227), Expect = 1.334e-27
Identity = 51/128 (39.84%), Postives = 72/128 (56.25%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRK--LIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSR 545
            P F K FL+  +A   G GAVL Q D  G  +   Y S     H  NY+ IE EALA++ A+  F  Y+ G    ++V+TDH PL  L++      +L+R AL+LQ Y +E+ + K  +N  ADA+SR
Sbjct: 1214 PDFGKPFLLEVDASDVGAGAVLLQEDSDGILHPTCYYSHKFNRHQCNYSTIEKEALALILAVQHFEVYVGGSSFPVVVFTDHNPLVFLSRMFNQNQRLMRWALLLQDYQLEVRHKKGVENVVADALSR 1341

HSP 2 Score: 52.373 bits (124), Expect = 1.334e-27
Identity = 24/56 (42.86%), Postives = 34/56 (60.71%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             + GYYR+F + FS +  PLT+L    V+F+W    + AF ++K LL E PIL  P
Sbjct: 1159 GMAGYYRRFCKNFSDVVEPLTKLLSPKVEFMWSPACEHAFTSVKILLTEAPILLAP 1214
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Cavefish
Match: ENSAMXT00000045638.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000061.1:953511:958244:1 gene:ENSAMXG00000040892.1 transcript:ENSAMXT00000045638.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.2041 bits (230), Expect = 3.488e-27
Identity = 52/138 (37.68%), Postives = 79/138 (57.25%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKL--IVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQ 566
            ++ P F++ FL+  +A   G GAVL Q D  G  + + + S     + +NY+ IE EALA++ AI  F  Y+    L  +V+TDH PL  L++      +L+R AL+LQ Y++EI + K  +N  ADA+SR     EQ
Sbjct: 1440 LSAPDFSRPFLLEVDASAVGAGAVLLQEDADGILHPVCFYSHKFTSYQRNYSTIEKEALALLLAIQHFEVYVGSSSLPVVVFTDHNPLVFLSRMYNKNQRLMRWALLLQDYNLEIRHKKGMENVVADALSRAADSCEQ 1577

HSP 2 Score: 49.6766 bits (117), Expect = 3.488e-27
Identity = 23/56 (41.07%), Postives = 31/56 (55.36%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             +  YYR+F R FS +  PLT L   +V F W S    AF ++K LL + P+L  P
Sbjct: 1388 GMASYYRRFCRNFSAVVQPLTSLLSPSVNFKWSSACDHAFTSVKILLSDAPVLSAP 1443
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Cavefish
Match: ENSAMXT00000039316.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:30933509:30934681:1 gene:ENSAMXG00000029182.1 transcript:ENSAMXT00000039316.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.1889 bits (204), Expect = 1.805e-26
Identity = 49/129 (37.98%), Postives = 71/129 (55.04%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSR 545
            +TFP   + F++ T+A    IGA+L Q+ D G E  +AYASR L    KNYA  + E LA+V+  + FR YL GR+ ++ TDH  L  L   +    +L      L  +D EI +     + NAD++SR
Sbjct:  151 LTFPDPGQTFILDTDASDVAIGAILSQKID-GFEKVVAYASRALSRQEKNYATTKKELLAVVHFTSYFRHYLLGRRFLLRTDHSSLRWLHNFSQPEGQLACWLEQLAQFDYEIEHRPGKKHVNADSLSR 278

HSP 2 Score: 56.9954 bits (136), Expect = 1.805e-26
Identity = 28/56 (50.00%), Postives = 33/56 (58.93%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYR+F+  F+ +A PL +L  K V F W  E Q AF TLK  LV  PIL FP
Sbjct:   99 GLASYYRRFVSGFAELARPLHKLTEKGVSFKWTQECQTAFQTLKDKLVSAPILTFP 154
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Cavefish
Match: ENSAMXT00000039384.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:21146036:21149703:-1 gene:ENSAMXG00000035834.1 transcript:ENSAMXT00000039384.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 2.886e-26
Identity = 50/129 (38.76%), Postives = 72/129 (55.81%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSR 545
            +TFP   + F++ T+A    IGA+L Q+ D G E  +AYASR L    KNYA  + E LA+V+  + FR YL GR+ ++ TDH  L  L   +    +L R    L  +D EI +     + NAD++SR
Sbjct:  139 LTFPDPGQTFILDTDASDVAIGAILSQKID-GFEKVVAYASRALSRQEKNYATTKKELLAVVHFTSYFRHYLLGRRFLLRTDHSSLRWLHNFSQPEGQLARWLEQLAQFDYEIEHRPGKKHVNADSLSR 266

HSP 2 Score: 55.4546 bits (132), Expect = 2.886e-26
Identity = 28/56 (50.00%), Postives = 33/56 (58.93%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYR+F+  F+ +A PL +L  K V F W  E Q AF TLK  LV  PIL FP
Sbjct:   87 GLASYYRRFVSGFAELARPLHKLTEKGVSFKWTQECQTAFQTLKDKLVSAPILTFP 142
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Medaka
Match: ENSORLT00000031051.1 (uncharacterized LOC111947296 [Source:NCBI gene;Acc:111947296])

HSP 1 Score: 96.2857 bits (238), Expect = 3.699e-31
Identity = 50/132 (37.88%), Postives = 82/132 (62.12%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLI---VYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSR 545
            ++ P F+K F +  +A   G+GAVL Q DD G ++ ++Y S+ L  H K Y+ IE EAL+++ A+  F  Y+ G   +   V+TDH PL  L + +    +L+R AL +Q Y++E+H+ + ++N  ADA+SR
Sbjct: 1441 LSAPVFSKPFKLEIDASADGVGAVLLQEDDSGIDHPVSYFSKKLNDHQKRYSTIEKEALSLLLALQHFEVYV-GSSTVPVKVFTDHSPLVFLRRMSNHNQRLMRWALFIQDYNLEMHHKRGSENVIADALSR 1571

HSP 2 Score: 59.6918 bits (143), Expect = 3.699e-31
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFPVL 177
             + GYYR+F R FS++A PL+ L      F+W +E Q AFD LK +L  PP+L  PV 
Sbjct: 1389 GMAGYYRRFCRNFSSVASPLSALTSPLKPFIWSNECQHAFDGLKAMLCCPPVLSAPVF 1446
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Medaka
Match: ENSORLT00000041026.1 (pep primary_assembly:ASM223467v1:6:22718848:22725332:1 gene:ENSORLG00000029874.1 transcript:ENSORLT00000041026.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 96.2857 bits (238), Expect = 4.084e-31
Identity = 50/132 (37.88%), Postives = 82/132 (62.12%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLI---VYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSR 545
            ++ P F+K F +  +A   G+GAVL Q DD G ++ ++Y S+ L  H K Y+ IE EAL+++ A+  F  Y+ G   +   V+TDH PL  L + +    +L+R AL +Q Y++E+H+ + ++N  ADA+SR
Sbjct: 1443 LSAPVFSKPFKLEIDASADGVGAVLLQEDDSGIDHPVSYFSKKLNDHQKRYSTIEKEALSLLLALQHFEVYV-GSSTVPVKVFTDHSPLVFLRRMSNHNQRLMRWALFIQDYNLEMHHKRGSENVIADALSR 1573

HSP 2 Score: 59.6918 bits (143), Expect = 4.084e-31
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFPVL 177
             + GYYR+F R FS++A PL+ L      F+W +E Q AFD LK +L  PP+L  PV 
Sbjct: 1391 GMAGYYRRFCRNFSSVASPLSALTSPLKPFIWSNECQHAFDGLKAMLCCPPVLSAPVF 1448
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Medaka
Match: ENSORLT00000041405.1 (pep primary_assembly:ASM223467v1:5:14130243:14134904:1 gene:ENSORLG00000026873.1 transcript:ENSORLT00000041405.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.8929 bits (224), Expect = 2.898e-29
Identity = 50/129 (38.76%), Postives = 75/129 (58.14%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P+F+K F +  +A     GAVL Q D  G ++ + Y SR    H  NY+ IE E LA++ A+  F  YL      ++VYTDH PL  L+       +L+R AL++Q +++EI + K +DN  ADA+SR+
Sbjct: 1425 PNFSKSFKLEVDASGVAAGAVLLQEDSEGIDHPVCYFSRKFAKHQINYSTIEKETLALLMALQHFEVYLGSSAVPVLVYTDHNPLTFLSSMYNHNQRLMRWALVIQDFNLEIRHKKGSDNVLADALSRV 1553

HSP 2 Score: 58.9214 bits (141), Expect = 2.898e-29
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             + GYYR F R FS + +PLT L   +  F W  E Q AFD+LK LL   P+L  P
Sbjct: 1370 GMAGYYRAFCRNFSAVVYPLTHLLSPSTAFTWTPECQHAFDSLKTLLTHAPVLAAP 1425
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Medaka
Match: ENSORLT00000035259.1 (pep primary_assembly:ASM223467v1:7:7197276:7201937:-1 gene:ENSORLG00000026629.1 transcript:ENSORLT00000035259.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.8929 bits (224), Expect = 2.971e-29
Identity = 50/129 (38.76%), Postives = 75/129 (58.14%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P+F+K F +  +A     GAVL Q D  G ++ + Y SR    H  NY+ IE E LA++ A+  F  YL      ++VYTDH PL  L+       +L+R AL++Q +++EI + K +DN  ADA+SR+
Sbjct: 1425 PNFSKSFKLEVDASGVAAGAVLLQEDSEGIDHPVCYFSRKFAKHQINYSTIEKETLALLMALQHFEVYLGSSAVPVLVYTDHNPLTFLSSMYNHNQRLMRWALVIQDFNLEIRHKKGSDNVLADALSRV 1553

HSP 2 Score: 58.9214 bits (141), Expect = 2.971e-29
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             + GYYR F R FS + +PLT L   +  F W  E Q AFD+LK LL   P+L  P
Sbjct: 1370 GMAGYYRAFCRNFSAVVYPLTHLLSPSTAFTWTPECQHAFDSLKTLLTHAPVLAAP 1425
[Close]
BLAST of Gag-pol fusion protein vs. Ensembl Medaka
Match: ENSORLT00000039038.1 (pep primary_assembly:ASM223467v1:16:12496065:12501960:-1 gene:ENSORLG00000023181.1 transcript:ENSORLT00000039038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.5077 bits (223), Expect = 3.121e-29
Identity = 50/129 (38.76%), Postives = 75/129 (58.14%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGR--KLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRM 548
            P+F+K F +  +A     GAVL Q D  G ++ + Y SR    H  NY+ IE E LA++ A+  F  YL      ++VYTDH PL  L+       +L+R AL++Q +++EI + K +DN  ADA+SR+
Sbjct: 1425 PNFSKSFKLEVDASGVAAGAVLLQEDSEGIDHPVCYFSRKFAKHQINYSTIEKETLALLMALQHFEVYLGSSAVPVLVYTDHNPLTFLSSMYNHNQRLMRWALVIQDFNLEIRHKKGSDNVLADALSRV 1553

HSP 2 Score: 58.9214 bits (141), Expect = 3.121e-29
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             + GYYR F R FS + +PLT L   +  F W  E Q AFD+LK LL   P+L  P
Sbjct: 1370 GMAGYYRAFCRNFSAVVYPLTHLLSPSTAFTWTPECQHAFDSLKTLLTHAPVLAAP 1425
[Close]
BLAST of Gag-pol fusion protein vs. Planmine SMEST
Match: SMESG000036597.1 (SMESG000036597.1)

HSP 1 Score: 351.673 bits (901), Expect = 5.809e-115
Identity = 179/203 (88.18%), Postives = 180/203 (88.67%), Query Frame = 3 
Query: 1443 IRWYIVPLKNKYQVKHEMDVPFGSTVAVPRSPTDSSLHEHPRDVCEQIKLLTVFPEFNQSTVTSYLWIHSINKPYTIFIPGATGPILRQLESTLEVHNKDKPEKNVTIQCNAKNTTVVLKILEPVKIPQLVITQVVISSTVHVTLKKIAKVEKEELLRAHLSKTRHLQNYRKLLREKHLHFRQFSWQMKTNMPHVENKMMILP 2051
            IRWYIVPLKNKYQVKHE DVPFGSTVAVPRSPTDS                    +FNQSTVTSYLWIHSINKPYTIFIPGATGPILRQLESTLEVHNKDKPEKNVTIQCNAKNTTVVLKILEPVKIPQLVITQVVISSTVHVTLKKIAKVEKEELLRAHL KTRH QNYRKLLREKHLHFRQFSWQMKTNMPHVENKMMILP
Sbjct:   57 IRWYIVPLKNKYQVKHERDVPFGSTVAVPRSPTDS--------------------KFNQSTVTSYLWIHSINKPYTIFIPGATGPILRQLESTLEVHNKDKPEKNVTIQCNAKNTTVVLKILEPVKIPQLVITQVVISSTVHVTLKKIAKVEKEELLRAHLPKTRHPQNYRKLLREKHLHFRQFSWQMKTNMPHVENKMMILP 239
[Close]
BLAST of Gag-pol fusion protein vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 153.295 bits (386), Expect = 9.983e-47
Identity = 99/254 (38.98%), Postives = 137/254 (53.94%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFVV----------------------LRENQSTCRCNGSSESRIGDQ---VMLESL**KEVITQIDGKF*RPYEVLRTTETNLILQLVANRKVESIMVHANRCKKCVVSD 854
            P+F   F++MT+A     GAVLGQ+ +   ++ IAYASR LKPH KNY+ IE E LA+VY I  FR YL GRK +V T H PL+ L KH    SKL+R ++ LQ YD EI Y     N NAD +SR+   +E+++  E     TIF+                       LR++  T +   S    + D+    ++     K +  ++   F  PY V  TTETNL L+   N+K  +I+VHANRCKK  V++
Sbjct: 2162 PNFRIPFILMTDASNFAFGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALVYGIATFRQYLVGRKFVVLTYHNPLQWLMKHWDSASKLIRWSIALQEYDFEIKYRTGKSNGNADTLSRIPVNNERKN-NEMNYNNTIFMAIKTANNLQELQKDQENDDELNQLRKHAVTIK-KESDWKHVADEKHKYIIHDEPAKGLSPKLQRPFKGPYIVCDTTETNLKLRPQNNKKPGTIIVHANRCKKVPVAE 2413

HSP 2 Score: 54.299 bits (129), Expect = 9.983e-47
Identity = 27/56 (48.21%), Postives = 35/56 (62.50%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYRKFI+ FS +A  L  L  K ++  WG  QQEAF+ LK+ L++ PIL  P
Sbjct: 2107 GLASYYRKFIKSFSHVAQLLNVLLKKQIQSKWGKAQQEAFELLKESLIKKPILRCP 2162
[Close]
BLAST of Gag-pol fusion protein vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 153.295 bits (386), Expect = 1.149e-46
Identity = 99/254 (38.98%), Postives = 137/254 (53.94%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFVV----------------------LRENQSTCRCNGSSESRIGDQ---VMLESL**KEVITQIDGKF*RPYEVLRTTETNLILQLVANRKVESIMVHANRCKKCVVSD 854
            P+F   F++MT+A     GAVLGQ+ +   ++ IAYASR LKPH KNY+ IE E LA+VY I  FR YL GRK +V T H PL+ L KH    SKL+R ++ LQ YD EI Y     N NAD +SR+   +E+++  E     TIF+                       LR++  T +   S    + D+    ++     K +  ++   F  PY V  TTETNL L+   N+K  +I+VHANRCKK  V++
Sbjct: 2152 PNFRIPFILMTDASNFAFGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALVYGIATFRQYLVGRKFVVLTYHNPLQWLMKHWDSASKLIRWSIALQEYDFEIKYRTGKSNGNADTLSRIPVNNERKN-NEMNYNNTIFMAIKTANNLQELQKDQENDDELNQLRKHAVTIK-KESDWKHVADEKHKYIIHDEPAKGLSPKLQRPFKGPYIVCDTTETNLKLRPQNNKKPGTIIVHANRCKKVPVAE 2403

HSP 2 Score: 54.299 bits (129), Expect = 1.149e-46
Identity = 27/56 (48.21%), Postives = 35/56 (62.50%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYRKFI+ FS +A  L  L  K ++  WG  QQEAF+ LK+ L++ PIL  P
Sbjct: 2097 GLASYYRKFIKSFSHVAQLLNVLLKKQIQSKWGKAQQEAFELLKESLIKKPILRCP 2152
[Close]
BLAST of Gag-pol fusion protein vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 132.88 bits (333), Expect = 1.318e-40
Identity = 70/150 (46.67%), Postives = 94/150 (62.67%), Query Frame = 3 
Query:  168 PSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSRMDQQDEQEDVKEGRLERTIFVVLR 617
            P+F   F++MT+A     GAVLGQ+ +   ++ IAYASR LKPH KNY+ IE E LA+VY I  FR YL GRK +V T H PL+ L KH    SKL+R ++ LQ YD EI Y     N NAD +SR+   +E+++  E     TIF+ ++
Sbjct: 2162 PNFRIPFILMTDASNFAFGAVLGQQVEGEKDHVIAYASRTLKPHEKNYSTIEKETLALVYGIATFRQYLVGRKFVVLTYHNPLQWLMKHWDSASKLIRWSIALQEYDFEIKYRTGKSNGNADTLSRIPVNNERKN-NEMNYNNTIFMAIK 2310

HSP 2 Score: 54.6842 bits (130), Expect = 1.318e-40
Identity = 27/56 (48.21%), Postives = 35/56 (62.50%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L  YYRKFI+ FS +A  L  L  K ++  WG  QQEAF+ LK+ L++ PIL  P
Sbjct: 2107 GLASYYRKFIKSFSHVAQLLNVLLKKQIQSKWGKAQQEAFELLKESLIKKPILRCP 2162
[Close]
BLAST of Gag-pol fusion protein vs. Planmine SMEST
Match: SMESG000010523.1 (SMESG000010523.1)

HSP 1 Score: 110.923 bits (276), Expect = 4.340e-37
Identity = 62/138 (44.93%), Postives = 78/138 (56.52%), Query Frame = 3 
Query:  159 ITFPSFAKQFLVMTEARTPGIGAVLGQRDDLGDEYAIAYASRGLKPH*KNYAVIELEALAIVYAITQFRTYLWGRKLIVYTDH*PLELLTKHTMDTSKLVRLALILQPYDIEIHYHKRNDNENADAVSR------MDQ 554
            +  P F K FL+ T+A     GAVLGQ DD   E  I Y SR  K H KNY+V E EALAI+ AI  F+  LWG ++ + TDH PL  L +H   +S+L+R A+ LQ Y   I +     N NAD +SR      MDQ
Sbjct: 2063 LVHPDFKKPFLLATDASGYASGAVLGQWDDEKRERVIGYYSRTFKKHEKNYSVTEREALAIIQAIKHFKYLLWGHEIYITTDHQPLVWLGQHKEASSRLMRWAMQLQEYSPYIKFKSGKANANADCMSRFVFEELMDQ 2200

HSP 2 Score: 64.6994 bits (156), Expect = 4.340e-37
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = 1 
Query:    4 ALVGYYRKFIRIFSTIAFPLTQLEGKNVKFVWGSEQQEAFDTLKKLLVEPPILPFP 171
             L GYYRKFI+ ++TIA P+ +L  K+  F+W  EQQ AF+TL+  L+  PIL  P
Sbjct: 2011 GLCGYYRKFIKSYATIAKPIQELTKKDTPFIWEEEQQTAFETLRDKLISAPILVHP 2066
The following BLAST results are available for this feature:
BLAST of Gag-pol fusion protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX890543.13.139e-2130.07pep chromosome:GRCz11:14:42224893:42231293:-1 gene... [more]
BX511082.14.931e-2132.00pep chromosome:GRCz11:9:14291932:14297132:1 gene:E... [more]
BX546500.11.326e-1933.85pep chromosome:GRCz11:23:12926092:12931693:-1 gene... [more]
CR855320.19.954e-1930.67pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENS... [more]
CR925755.22.242e-1830.67pep chromosome:GRCz11:17:42486740:42492668:-1 gene... [more]
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BLAST of Gag-pol fusion protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSXETT00000024810.12.026e-3045.04pep primary_assembly:Xenopus_tropicalis_v9.1:KV464... [more]
ENSXETT00000011934.11.702e-2539.37pep primary_assembly:Xenopus_tropicalis_v9.1:3:519... [more]
anxa63.195e-2539.29annexin A6 [Source:Xenbase;Acc:XB-GENE-989741][more]
ENSXETT00000031144.13.094e-1335.29pep primary_assembly:Xenopus_tropicalis_v9.1:8:632... [more]
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BLAST of Gag-pol fusion protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P10394|POL4_DROME5.199e-2739.23Retrovirus-related Pol polyprotein from transposon... [more]
sp|P04323|POL3_DROME8.108e-2541.73Retrovirus-related Pol polyprotein from transposon... [more]
sp|P20825|POL2_DROME5.209e-2339.23Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q8I7P9|POL5_DROME8.715e-1938.04Retrovirus-related Pol polyprotein from transposon... [more]
sp|P10401|POLY_DROME4.463e-1231.65Retrovirus-related Pol polyprotein from transposon... [more]
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BLAST of Gag-pol fusion protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A355ABF21.948e-4144.79Uncharacterized protein OS=Flavobacteriaceae bacte... [more]
A0A1X7UNW98.553e-3542.86Uncharacterized protein OS=Amphimedon queenslandic... [more]
A0A2H5TX349.820e-3542.31Gag-pol fusion protein OS=Rhizophagus irregularis ... [more]
A0A2H5S0291.566e-3444.09Retrotransposable element OS=Rhizophagus irregular... [more]
A0A2H5RIZ51.893e-3444.09Retrotransposable element OS=Rhizophagus irregular... [more]
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BLAST of Gag-pol fusion protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000041345.11.405e-2841.54pep primary_assembly:Astyanax_mexicanus-2.0:25:323... [more]
ENSAMXT00000051014.11.334e-2739.84pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000045638.13.488e-2737.68pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000039316.11.805e-2637.98pep primary_assembly:Astyanax_mexicanus-2.0:15:309... [more]
ENSAMXT00000039384.12.886e-2638.76pep primary_assembly:Astyanax_mexicanus-2.0:25:211... [more]
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BLAST of Gag-pol fusion protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Gag-pol fusion protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000031051.13.699e-3137.88uncharacterized LOC111947296 [Source:NCBI gene;Acc... [more]
ENSORLT00000041026.14.084e-3137.88pep primary_assembly:ASM223467v1:6:22718848:227253... [more]
ENSORLT00000041405.12.898e-2938.76pep primary_assembly:ASM223467v1:5:14130243:141349... [more]
ENSORLT00000035259.12.971e-2938.76pep primary_assembly:ASM223467v1:7:7197276:7201937... [more]
ENSORLT00000039038.13.121e-2938.76pep primary_assembly:ASM223467v1:16:12496065:12501... [more]
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BLAST of Gag-pol fusion protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000036597.15.809e-11588.18SMESG000036597.1[more]
SMESG000057446.19.983e-4738.98SMESG000057446.1[more]
SMESG000057446.11.149e-4638.98SMESG000057446.1[more]
SMESG000057446.11.318e-4046.67SMESG000057446.1[more]
SMESG000010523.14.340e-3744.93SMESG000010523.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30007028 ID=SMED30007028|Name=Gag-pol fusion protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2766bp
AGGGCTTTGGTGGGTTATTATAGGAAGTTCATCCGAATTTTCTCGACCAT
AGCATTTCCTCTCACTCAGTTAGAAGGTAAAAATGTGAAATTTGTATGGG
GCTCAGAGCAACAAGAAGCGTTCGATACACTCAAGAAATTATTGGTGGAA
CCTCCAATATTACCTTTCCCAGTTTTGCCAAGCAATTCTTAGTCATGACA
GAAGCCAGAACTCCGGGTATTGGTGCTGTACTAGGTCAGAGAGATGACCT
AGGCGACGAGTATGCAATTGCATACGCCAGCCGAGGTTTGAAACCTCACT
AGAAAAATTATGCAGTAATCGAATTAGAAGCACTAGCAATTGTGTATGCT
ATAACCCAGTTTAGAACATACCTATGGGGACGAAAGCTAATCGTGTATAC
TGATCATTGACCTTTAGAATTGCTAACCAAACATACTATGGATACATCCA
AGCTGGTGAGATTGGCACTAATTTTGCAGCCATATGATATAGAGATTCAC
TATCATAAAAGGAATGATAACGAAAACGCGGACGCAGTTTCCCGAATGGA
TCAACAAGATGAGCAGGAAGATGTCAAAGAAGGTAGATTGGAAAGAACCA
TATTCGTGGTACTCAGAGAAAACCAATCAACTTGTAGATGTAATGGAAGC
TCAGAAAGCAGAATAGGAGATCAAGTAATGTTAGAGTCACTATAATAAAA
AGAGGTTATTACCCAAATTGATGGTAAATTTTAAAGACCATATGAAGTCT
TACGAACAACCGAAACAAATTTGATTCTACAGCTTGTAGCAAATAGAAAA
GTGGAGTCAATTATGGTTCACGCGAATCGTTGTAAAAAGTGTGTTGTTTC
TGATAAGAAGAATGTATTACCTGGAAAACCGTTCAACGAGAAAACAAAGA
ATGTCATTGAAGAAACTCCGGAGCACCGAAATCCATTACGGTCAAGAGGT
GCTCCAAAATCAGTATCATTTTAAGCTATTTGCTGTTTAATTATGATGTC
AACTCAAAAACAATTGCCAACAATGTTCGAGCCCATTGCATTACAGACCA
ACCATTTACATTTTGCGAGAACGTATCAAGGATTTAGAATCTTTACATCA
TGTAACAATCGGACGAAGTTCTCCATCTATTTCTGTGTGGATTATTGCTC
ATCATTTTAGCTAGACTCAACAAATAGGCAAAAAATACCTTTATAATTTA
AAGACAGAACATAATTATCTGACGGAAATTATGGAACACTTCTATACCTG
TCAGCTTAGTAAAGCGATAAAATTAAGCATAATTAGATATCATAAGACGA
GATTTATGCATGTGTTTTGCCAGTAATGGCTTATCAGTGCATCTGATAGT
GTGCTTAATCATTTATTTTGTTGAAATTAATCACCTAATTTCACAATATC
AATACTGACACAGTTCAATTGAAGTAAATACCTTACTCAAAGATAAGATG
GTATATCGTACCATTGAAAAACAAATATCAAGTGAAGCATGAAATGGACG
TACCGTTTGGTTCTACAGTAGCTGTACCCAGATCCCCAACGGATTCAAGT
TTGCACGAGCACCCAAGAGACGTGTGTGAGCAAATCAAACTATTAACTGT
TTTCCCAGAATTTAACCAATCTACGGTTACTTCCTATTTATGGATTCACT
CTATCAATAAACCTTATACCATTTTTATTCCTGGAGCCACAGGACCAATA
TTGCGCCAACTGGAATCCACTCTCGAAGTTCACAATAAAGACAAACCGGA
AAAGAATGTAACTATTCAGTGCAATGCTAAGAACACCACTGTGGTACTAA
AAATATTAGAGCCGGTAAAAATCCCGCAGCTAGTCATCACGCAAGTAGTC
ATTTCCTCAACAGTTCATGTGACACTGAAGAAAATAGCGAAAGTGGAAAA
AGAAGAGCTATTAAGGGCTCATCTTTCGAAAACCAGACATCTTCAAAATT
ATAGAAAATTGTTAAGGGAGAAACATTTACACTTCCGACAGTTTTCGTGG
CAGATGAAGACGAATATGCCACACGTGGAGAATAAGATGATGATTTTACC
CTAAATATCCATATCCCGAAAGAAGTTGCATCAGTAACCGACATCGTTAA
GAGGTTTACCAATAAATTTTAAGTTAGATCAGTGACAAGAAAAAATAGTA
CCAGCAATTTCTTACACGAAATAAGTCAAGCCTGTAACCGACGAAAGGTT
TATCACAAAAAAATCGAAGCCCTTAAATCAGTGACCAAAGAGAAAATAAT
ACTAGTGGGTTCTTACCCGAAGAAGGAAGTCAAACCATTAGTCGACAAGA
AATTCAAGGACCAGAGGCCTGTGACAAAGAAGAAAAAATAATGCCAGCCT
TGTCAATCCAAGACGAGGATGACGACCTGTTAAAAGTGAACTATAAACCG
CCCAAGCCAGAATTGACACAAGCTTTGAAAGTAAAGGCTGAACCCCAGGA
AGTCACTGAAGGAAATTTTGTTGCGTCGGGATGATGCTAGAAAGATGCTG
TTGACCTAGACAGAGAAGGTCCTAGATGGTGGCAGAGACCAGGAGCCAAC
CGGAAACATTGACTTAAAGAATATGATGGTGAACCGGTGTACAAGATGGC
GGTCTCAAACTTCTCGATTCGATCTGTTATGGGACGTACGTAATATCCTC
CATTATATATCGAGAAGTCACAACAAGAAAGAATCTTGTTAATAGTTATG
GGCCCGATATAATCGTAGAACGAATGGCATTTGAGAGTGCAACAGGATTC
GAGAAAGACGAAAGTA
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protein sequence of SMED30007028-orf-1

>SMED30007028-orf-1 ID=SMED30007028-orf-1|Name=SMED30007028-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=187bp
MDVPFGSTVAVPRSPTDSSLHEHPRDVCEQIKLLTVFPEFNQSTVTSYLW
IHSINKPYTIFIPGATGPILRQLESTLEVHNKDKPEKNVTIQCNAKNTTV
VLKILEPVKIPQLVITQVVISSTVHVTLKKIAKVEKEELLRAHLSKTRHL
QNYRKLLREKHLHFRQFSWQMKTNMPHVENKMMILP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0003676nucleic acid binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR041577RT_RNaseH_2
Vocabulary: biological process
TermDefinition
GO:0015074DNA integration
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 8..67
e-value: 8.6E-9
score: 35.3